Yeast Colony Group

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  • NH3 production measurement :

Ammonia released by growing colonies is absorbed into acidic traps (CitA, 10% citric acid) in particular intervals of colony development. Nitrogen content in CitA traps is measured using the Nessler reagent. 

NH3 Production Measurement


 
 

  • Induction of colonies by ammonia released from artificial source :

Ammonia is generated in a small vessel containing NH4Cl (70 mg) and 1 M NaOH (70 ml). The vessel is placed on the plate as indicated on the figure, in particular times of colony development.

Induction of colonies