Methods used in research<\/h2>\n\n\n\n![\"\"](\"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2022\/09\/Figure_11_CD3zeta-A2.png\")
<\/figure>\nStructural research of complexes of NK cell receptors and their ligands<\/strong><\/h3>\n\n\n\nRecombinant protein expression within the HEK293 human cell line<\/p>\n\n\n\n
Biophysical characterization of proteins and their interactions (AUC, ITC, MST, DLS, DSF, SPR)<\/p>\n\n\n\n
Protein crystallography, structural mass spectrometry, SAXS, cryoEM<\/p>\n<\/div><\/div>\n\n\n\n
<\/div>\n\n\n\n\nResearch of NK cell receptors’ arrangement on the surface of a living cell<\/strong><\/h3>\n\n\n\nSuperresolution microscopy, fluorescence microscopy, flow cytometry, single-molecule localization microscopy (SMLM)<\/p>\n<\/div>![\"\"](\"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2022\/09\/Fig_3-1024x1024.jpg\")
<\/figure><\/div>\n\n\n\n<\/div>\n\n\n\n![\"\"](\"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2022\/09\/IL-stuf_2-1024x974.png\")
<\/figure>\nProduction of fusion constructs with IL-2 or growth factors<\/strong><\/h3>\n\n\n\nExpression, purification, and study of properties of fusion proteins containing protein hormones or cytokines<\/p>\n<\/div><\/div>\n\n\n\n
<\/div>\n\n\n\n\nProduction of fusion constructs of nanobodies with ligands of activation receptors of NK cells and testing their biological activity<\/strong><\/h3>\n\n\n\nIsolation of NK cells from the blood of healthy donors<\/p>\n\n\n\n
Cytotoxicity tests of NK cells against tumour cell lines<\/p>\n<\/div>![\"\"](\"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2022\/03\/IMG_0331-1024x768.jpg\")
<\/figure><\/div>\n\n\n\n\n\n<\/p>\n\n\n\n
<\/p>\n","protected":false},"excerpt":{"rendered":"
We are a well-functioning research team pursuing exciting projects bringing health and prosperity! Our research interest Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-4903","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false},"uagb_author_info":{"display_name":"structimmuno","author_link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/author\/structimmuno\/"},"uagb_comment_info":0,"uagb_excerpt":"We are a well-functioning research team pursuing exciting projects bringing health and prosperity! Our research interest Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor,…","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/comments?post=4903"}],"version-history":[{"count":2,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903\/revisions"}],"predecessor-version":[{"id":7384,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903\/revisions\/7384"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media?parent=4903"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/figure>Structural research of complexes of NK cell receptors and their ligands<\/strong><\/h3>\n\n\n\nRecombinant protein expression within the HEK293 human cell line<\/p>\n\n\n\n
Biophysical characterization of proteins and their interactions (AUC, ITC, MST, DLS, DSF, SPR)<\/p>\n\n\n\n
Protein crystallography, structural mass spectrometry, SAXS, cryoEM<\/p>\n<\/div><\/div>\n\n\n\n
<\/div>\n\n\n\n\nResearch of NK cell receptors’ arrangement on the surface of a living cell<\/strong><\/h3>\n\n\n\nSuperresolution microscopy, fluorescence microscopy, flow cytometry, single-molecule localization microscopy (SMLM)<\/p>\n<\/div><\/figure><\/div>\n\n\n\n<\/div>\n\n\n\n<\/figure>\nProduction of fusion constructs with IL-2 or growth factors<\/strong><\/h3>\n\n\n\nExpression, purification, and study of properties of fusion proteins containing protein hormones or cytokines<\/p>\n<\/div><\/div>\n\n\n\n
<\/div>\n\n\n\n\nProduction of fusion constructs of nanobodies with ligands of activation receptors of NK cells and testing their biological activity<\/strong><\/h3>\n\n\n\nIsolation of NK cells from the blood of healthy donors<\/p>\n\n\n\n
Cytotoxicity tests of NK cells against tumour cell lines<\/p>\n<\/div><\/figure><\/div>\n\n\n\n\n\n<\/p>\n\n\n\n
<\/p>\n","protected":false},"excerpt":{"rendered":"
We are a well-functioning research team pursuing exciting projects bringing health and prosperity! Our research interest Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-4903","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false},"uagb_author_info":{"display_name":"structimmuno","author_link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/author\/structimmuno\/"},"uagb_comment_info":0,"uagb_excerpt":"We are a well-functioning research team pursuing exciting projects bringing health and prosperity! Our research interest Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor,…","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/comments?post=4903"}],"version-history":[{"count":2,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903\/revisions"}],"predecessor-version":[{"id":7384,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903\/revisions\/7384"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media?parent=4903"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Research of NK cell receptors’ arrangement on the surface of a living cell<\/strong><\/h3>\n\n\n\nSuperresolution microscopy, fluorescence microscopy, flow cytometry, single-molecule localization microscopy (SMLM)<\/p>\n<\/div><\/figure><\/div>\n\n\n\n<\/div>\n\n\n\n<\/figure>\nProduction of fusion constructs with IL-2 or growth factors<\/strong><\/h3>\n\n\n\nExpression, purification, and study of properties of fusion proteins containing protein hormones or cytokines<\/p>\n<\/div><\/div>\n\n\n\n
<\/div>\n\n\n\n\nProduction of fusion constructs of nanobodies with ligands of activation receptors of NK cells and testing their biological activity<\/strong><\/h3>\n\n\n\nIsolation of NK cells from the blood of healthy donors<\/p>\n\n\n\n
Cytotoxicity tests of NK cells against tumour cell lines<\/p>\n<\/div><\/figure><\/div>\n\n\n\n\n\n<\/p>\n\n\n\n
<\/p>\n","protected":false},"excerpt":{"rendered":"
We are a well-functioning research team pursuing exciting projects bringing health and prosperity! Our research interest Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-4903","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false},"uagb_author_info":{"display_name":"structimmuno","author_link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/author\/structimmuno\/"},"uagb_comment_info":0,"uagb_excerpt":"We are a well-functioning research team pursuing exciting projects bringing health and prosperity! Our research interest Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor,…","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/comments?post=4903"}],"version-history":[{"count":2,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903\/revisions"}],"predecessor-version":[{"id":7384,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903\/revisions\/7384"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media?parent=4903"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/figure><\/div>\n\n\n\n<\/figure>Production of fusion constructs with IL-2 or growth factors<\/strong><\/h3>\n\n\n\nExpression, purification, and study of properties of fusion proteins containing protein hormones or cytokines<\/p>\n<\/div><\/div>\n\n\n\n
<\/div>\n\n\n\n\nProduction of fusion constructs of nanobodies with ligands of activation receptors of NK cells and testing their biological activity<\/strong><\/h3>\n\n\n\nIsolation of NK cells from the blood of healthy donors<\/p>\n\n\n\n
Cytotoxicity tests of NK cells against tumour cell lines<\/p>\n<\/div><\/figure><\/div>\n\n\n\n\n\n<\/p>\n\n\n\n
<\/p>\n","protected":false},"excerpt":{"rendered":"
We are a well-functioning research team pursuing exciting projects bringing health and prosperity! Our research interest Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-4903","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false},"uagb_author_info":{"display_name":"structimmuno","author_link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/author\/structimmuno\/"},"uagb_comment_info":0,"uagb_excerpt":"We are a well-functioning research team pursuing exciting projects bringing health and prosperity! Our research interest Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor,…","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/comments?post=4903"}],"version-history":[{"count":2,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903\/revisions"}],"predecessor-version":[{"id":7384,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903\/revisions\/7384"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media?parent=4903"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Production of fusion constructs of nanobodies with ligands of activation receptors of NK cells and testing their biological activity<\/strong><\/h3>\n\n\n\nIsolation of NK cells from the blood of healthy donors<\/p>\n\n\n\n
Cytotoxicity tests of NK cells against tumour cell lines<\/p>\n<\/div><\/figure><\/div>\n\n\n\n\n\n<\/p>\n\n\n\n
<\/p>\n","protected":false},"excerpt":{"rendered":"
We are a well-functioning research team pursuing exciting projects bringing health and prosperity! Our research interest Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-4903","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false},"uagb_author_info":{"display_name":"structimmuno","author_link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/author\/structimmuno\/"},"uagb_comment_info":0,"uagb_excerpt":"We are a well-functioning research team pursuing exciting projects bringing health and prosperity! Our research interest Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor,…","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/comments?post=4903"}],"version-history":[{"count":2,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903\/revisions"}],"predecessor-version":[{"id":7384,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903\/revisions\/7384"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media?parent=4903"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/figure><\/div>\n\n\n\n\n\n<\/p>\n\n\n\n
<\/p>\n","protected":false},"excerpt":{"rendered":"
We are a well-functioning research team pursuing exciting projects bringing health and prosperity! Our research interest Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-4903","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false},"uagb_author_info":{"display_name":"structimmuno","author_link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/author\/structimmuno\/"},"uagb_comment_info":0,"uagb_excerpt":"We are a well-functioning research team pursuing exciting projects bringing health and prosperity! Our research interest Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor,…","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/comments?post=4903"}],"version-history":[{"count":2,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903\/revisions"}],"predecessor-version":[{"id":7384,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4903\/revisions\/7384"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media?parent=4903"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}