{"id":4983,"date":"2021-01-20T11:45:32","date_gmt":"2021-01-20T10:45:32","guid":{"rendered":"https:\/\/ukazky.euweb.cz\/?page_id=4983"},"modified":"2026-03-14T22:43:38","modified_gmt":"2026-03-14T22:43:38","slug":"publications","status":"publish","type":"page","link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/publications\/","title":{"rendered":"List of publications"},"content":{"rendered":"\n<div class=\"wp-block-columns are-vertically-aligned-center researcher-ID is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n<div class=\"wp-block-uagb-faq uagb-faq__outer-wrap uagb-block-b7754eff uagb-faq-icon-row uagb-faq-layout-accordion uagb-faq-expand-first-true uagb-faq-inactive-other-true uagb-faq__wrap uagb-buttons-layout-wrap uagb-faq-equal-height     \" data-faqtoggle=\"true\" role=\"tablist\"><script type=\"application\/ld+json\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@type\":\"FAQPage\",\"@id\":\"https:\\\/\\\/web.natur.cuni.cz\\\/biochem\\\/structimmuno\\\/publications\\\/\",\"mainEntity\":[{\"@type\":\"Question\",\"name\":\"2026\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Kapisheva M., Junkov\\u00e1 P., Van\\u011bk O., Jalovcov\\u00e1 Z., K\\u0159\\u00ed\\u017eov\\u00e1 I., Dost\\u00e1lkov\\u00e1 A., Rumlov\\u00e1 M. (2026): Protease-mediated maturation of M-PMV reverse transcriptase into a functional heterodimer. Protein Sci 35, e70469. <strong>IF 5.2<\\\/strong> DOI: <a href=\\\"https:\\\/\\\/doi.org\\\/10.1002\\\/pro.70469\\\" data-type=\\\"link\\\" data-id=\\\"https:\\\/\\\/doi.org\\\/10.1002\\\/pro.70469\\\">10.1002\\\/pro.70469<\\\/a> \"}},{\"@type\":\"Question\",\"name\":\"2025\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Di Carluccio C., Nieto-Fabregat F., Cerofolini L., Abreu C., Padilla-Cort\\u00e9s L., Gheorghita G. R., Masi A. A., Buono L., Ali M. G. A., Lamprinaki D., Molinaro A., Juge N., Smaldone G., Van\\u011bk O., Fragai M., Marchetti R., Silipo A. (2025): <em>Fusobacterium nucleatum<\\\/em> LPS O-antigen defines a novel Siglec-7 binding epitope. JACS Au\\u00a05, 5367-5380. <strong>IF 8.7<\\\/strong> DOI: <a href=\\\"https:\\\/\\\/doi.org\\\/10.1021\\\/jacsau.5c00810\\\" data-type=\\\"link\\\" data-id=\\\"https:\\\/\\\/doi.org\\\/10.1021\\\/jacsau.5c00810\\\">10.1021\\\/jacsau.5c00810<\\\/a> <br><br>La Gatta S., Leone L., Sgueglia G., \\u0160imuni\\u0107 L., Liao Y., Van\\u011bk O., Chino M., Nastri F., Chiesa M., Lombardi A. (2025): Engineering a functional histidine brace copper-binding site into a <em>de novo<\\\/em>-designed protein scaffold. JACS Au 5, 4799-4810. <strong>IF 8.7<\\\/strong> DOI: <a href=\\\"https:\\\/\\\/doi.org\\\/10.1021\\\/jacsau.5c00754\\\" data-type=\\\"link\\\" data-id=\\\"https:\\\/\\\/doi.org\\\/10.1021\\\/jacsau.5c00754\\\">10.1021\\\/jacsau.5c00754<\\\/a><br><br>Pachmayr I., Masullo L. A., Reinhardt S. C. M., Kwon J., Llop M., Sko\\u0159epa O., Herter S., Bacac M., Klein C., Jungmann R. (2025): Resolving the structural basis of therapeutic antibody function in cancer immunotherapy with RESI. Nat Commun 16, 6768. <strong>IF 15.7<\\\/strong> DOI: <a href=\\\"https:\\\/\\\/doi.org\\\/10.1038\\\/s41467-025-61893-w\\\" data-type=\\\"link\\\" data-id=\\\"https:\\\/\\\/doi.org\\\/10.1038\\\/s41467-025-61893-w\\\">10.1038\\\/s41467-025-61893-w<\\\/a><br><br>Di Carluccio C., Padilla-Cort\\u00e9s L., Tiemblo-Mart\\u00ecn M., Gheorghita G. R., Oliva R., Cerofolini L., Masi A. A., Abreu C., Tseng H. K., Molinaro A., Del Vecchio P., Van\\u011bk O., Lin C. C., Marchetti R., Fragai M., Silipo A. (2025): Insights into Siglec-7 binding to gangliosides: NMR protein assignment and the impact of ligand flexibility. Adv Sci 12, e2415782. <strong>IF 14.1<\\\/strong> DOI: <a href=\\\"https:\\\/\\\/doi.org\\\/10.1002\\\/advs.202415782\\\">10.1002\\\/advs.202415782<\\\/a><br><br>Di Carluccio C., Gerpe Amor T., Lenza M. P., Masi A. A., Abreu C., Longo V., Albano F., Nieto-Fabregat F., Salvatore P., Falco G., Santana-Medero D., Fragai M., van Kooyk Y., Molinaro A., Valdes-Balbin Y.,\\u00a0Van\\u011bk\\u00a0O., Verez-Bencomo V., Marchetti R., Chiodo F., Silipo A. (2025): Molecular basis of Siglec-7 recognition by\\u00a0<em>Neisseria meningitidis<\\\/em>\\u00a0serogroup Y CPS: Implications for immune evasion.\\u00a0<em>JACS Au<\\\/em>\\u00a05, 2257-2269.\\u00a0<strong>IF 8.7<\\\/strong>\\u00a0DOI:\\u00a0<a href=\\\"https:\\\/\\\/doi.org\\\/10.1021\\\/jacsau.5c00214\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1021\\\/jacsau.5c00214<\\\/a><br><br>Abreu C., Di Carluccio C., Je\\u010dmen T., Sko\\u0159epa O., Bl\\u00e1ha J., Marchetti R., Silipo A., Van\\u011bk O. (2025): Insights into stability, dimerisation, and ligand binding properties of Siglec-7: Isotope labelling in HEK293 cells for protein characterisation by NMR spectroscopy. <em>Int J Biol Macromol <\\\/em>309, 142672. <strong>IF 8.5<\\\/strong> DOI: <a href=\\\"https:\\\/\\\/doi.org\\\/10.1016\\\/j.ijbiomac.2025.142672\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1016\\\/j.ijbiomac.2025.142672<\\\/a><br><br>Nejadebrahim S., Houserov\\u00e1 J., Je\\u010dmen T., Kalouskov\\u00e1 B., Abreu C., Herynek \\u0160., Sko\\u0159epa O., Bl\\u00e1ha J., Van\\u011bk O. (2025): Multiple O- and an N-glycosylation of the stalk region of the NK cell activation receptor NKp46 mediates its interaction with the <em>Candida glabrata<\\\/em> epithelial adhesin 1. <em>Int J Biol Macromol<\\\/em> 310, 143037. <strong>IF 8.5<\\\/strong> DOI: <a class=\\\"\\\" href=\\\"https:\\\/\\\/doi.org\\\/10.1016\\\/j.ijbiomac.2025.143037\\\">10.1016\\\/j.ijbiomac.2025.143037<\\\/a>\"}},{\"@type\":\"Question\",\"name\":\"2024\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Leonard E. K., Tomala J., Gould J. R., Leff M. I., Lin J. X., Li P., Porter M. J., Johansen E. R., Thompson L., Cao S. D., Hou S., Henclov\\u00e1 T., Huli\\u010diak M., Sargunas P. R., Fabilane C. S., Van\\u011bk O., Kov\\u00e1\\u0159 M., Schneider B., Raimondi G., Leonard W. J., Spangler J. B. (2024): Engineered cytokine\\\/antibody fusion proteins improve IL-2 delivery to pro-inflammatory cells and promote antitumor activity. <em>JCI Insight<\\\/em> 9, e173469. <strong>IF 6.3<\\\/strong> DOI: <a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1172\\\/jci.insight.173469\\\">10.1172\\\/jci.insight.173469<\\\/a>\"}},{\"@type\":\"Question\",\"name\":\"2023\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Di Carluccio C., Milanesi F., Civera M., Abreu C., Sattin S., Francesconi O., Molinaro A., Van\\u011bk O., Marchetti R., Silipo A. (2023): Tumor carbohydrate associated antigen analogs as potential binders for Siglec-7. <em>Eur J Org Chem<\\\/em> 26, e202300644. <strong>IF 2.8<\\\/strong> DOI: <a href=\\\"https:\\\/\\\/doi.org\\\/10.1002\\\/ejoc.202300644\\\">10.1002\\\/ejoc.202300644<\\\/a>\"}},{\"@type\":\"Question\",\"name\":\"2022\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Anderluh M., Berti F., Bzducha-Wr\\u00f3bel A., Chiodo F., Colombo C., Compostella F., Durlik K., Ferhati X., Holmdahl R., Jovanovic D., Kaca W., Lay L., Marinovic-Cincovic M., Marradi M., Ozil M., Polito L., Reina-Martin J. J., Reis C. A., Sackstein R., Silipo A., \\u0160vajger U., Van\\u011bk O., Yamamoto F., Richichi B., van Vliet S. J. (2022): Recent advances on smart glycoconjugate vaccines in infections and cancer.\\u00a0<em>FEBS J<\\\/em>\\u00a0289, 4251\\u20134303.\\u00a0<strong>IF 5.622<\\\/strong> DOI:\\u00a0<a rel=\\\"noreferrer noopener\\\" href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1111\\\/febs.15909\\\" target=\\\"_blank\\\">10.1111\\\/febs.15909<\\\/a><br><br>Van\\u011bk O., Kalouskov\\u00e1 B., Abreu C., Nejadebrahim S., Sko\\u0159epa O. (2022): Natural killer cell-based strategies for immunotherapy of cancer.\\u00a0<em>Adv Protein Chem Struct Biol<\\\/em>\\u00a0129, 91-133.\\u00a0<strong>IF 5.447<\\\/strong> DOI:\\u00a0<a rel=\\\"noreferrer noopener\\\" href=\\\"https:\\\/\\\/doi.org\\\/10.1016\\\/bs.apcsb.2022.02.001\\\" target=\\\"_blank\\\">10.1016\\\/bs.apcsb.2022.02.001<\\\/a><br><br>Remans K., Lebendiker M., Abreu C., Maffei M., Sellathurai S., May M. M., Van\\u011bk O., de Marco A. (2022): Protein purification strategies must consider downstream applications and individual biological characteristics.\\u00a0<em>Microb Cell Fact<\\\/em>\\u00a021, 52.\\u00a0<strong>IF 6.352<\\\/strong> DOI:\\u00a0<a rel=\\\"noreferrer noopener\\\" href=\\\"https:\\\/\\\/doi.org\\\/10.1186\\\/s12934-022-01778-5\\\" target=\\\"_blank\\\">10.1186\\\/s12934-022-01778-5<\\\/a><br><br>Bl\\u00e1ha J., Sk\\u00e1lov\\u00e1 T., Kalouskov\\u00e1 B., Sko\\u0159epa O., Cmunt D., Grob\\u00e1rov\\u00e1 V., Pazicky S., Pol\\u00e1chov\\u00e1 E., Abreu C., Str\\u00e1nsk\\u00fd J., Kova\\u013e T., Du\\u0161kov\\u00e1 J., Zhao Y., Harlos K., Ha\\u0161ek J., Dohn\\u00e1lek J., Van\\u011bk O. (2022): Structure of the human NK cell NKR-P1:LLT1 receptor:ligand complex reveals clustering in the immune synapse.\\u00a0<em>Nat Commun<\\\/em>\\u00a013, 5022.\\u00a0<strong>IF 17.694<\\\/strong> DOI:\\u00a0<a rel=\\\"noreferrer noopener\\\" href=\\\"https:\\\/\\\/doi.org\\\/10.1038\\\/s41467-022-32577-6\\\" target=\\\"_blank\\\">10.1038\\\/s41467-022-32577-6<\\\/a>\"}},{\"@type\":\"Question\",\"name\":\"2021\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Stehl\\u00edk \\u0160., Henych J., \\u0160tenclov\\u00e1 P., Kr\\u00e1l R., Zemenov\\u00e1 P., Pangr\\u00e1c J., Van\\u011bk O., Kromka A., Rezek B. (2021): Size and nitrogen inhomogeneity in detonation and laser synthesized primary nanodiamond particles revealed via salt-assisted deaggregation.\\u00a0<em>Carbon<\\\/em>\\u00a0171, 230-239.\\u00a0<strong>IF 11.307<\\\/strong> DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1016\\\/j.carbon.2020.09.026\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1016\\\/j.carbon.2020.09.026<\\\/a><br><br>Zouharov\\u00e1 M., Vym\\u011btal J., Bedn\\u00e1rov\\u00e1 L., Van\\u011bk O., Herman P., Vet\\u00fd\\u0161kov\\u00e1 V., Po\\u0161tulkov\\u00e1 K., Lingstaadas P. S., Vondr\\u00e1\\u0161ek J., Bou\\u0161ov\\u00e1 K. (2021): Intrinsically disordered protein domain of human ameloblastin in synthetic fusion with calmodulin increases calmodulin stability and modulates its function.\\u00a0<em>Int J Biol Macromol<\\\/em>\\u00a0168, 1-12.\\u00a0<strong>IF 8.025<\\\/strong> DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1016\\\/j.ijbiomac.2020.11.216\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1016\\\/j.ijbiomac.2020.11.216<\\\/a><br><br>Stehl\\u00edk \\u0160., Mermoux M., Schummer B., Van\\u011bk O., Kol\\u00e1\\u0159ov\\u00e1 K., \\u0160tenclov\\u00e1 P., Vlk A., Ledinsk\\u00fd M., Pfeifer R., Romanyuk O., Gordeev I., Roussel-Dherbey F., N\\u011bme\\u010dkov\\u00e1 Z., Henych J., Bezdi\\u010dka P., Kromka A., Rezek B. (2021): Size effects on surface chemistry and Raman spectra of sub-5 nm oxidized high-pressure high-temperature and detonation nanodiamonds.\\u00a0<em>J Phys Chem C<\\\/em>\\u00a0125, 5647-5669.\\u00a0<strong>IF 4.189<\\\/strong> DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1021\\\/acs.jpcc.0c09190\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1021\\\/acs.jpcc.0c09190<\\\/a><br><br>Anderluh M., Berti F., Bzducha-Wr\\u00f3bel A., Chiodo F., Colombo C., Compostella F., Durlik K., Ferhati X., Holmdahl R., Jovanovic D., Kaca W., Lay L., Marinovic-Cincovic M., Marradi M., Ozil M., Polito L., Reina-Martin J. J., Reis C. A., Sackstein R., Silipo A., \\u0160vajger U., Van\\u011bk O., Yamamoto F., Richichi B., van Vliet S. J. (2021): Emerging glyco-based strategies to steer immune responses.\\u00a0<em>FEBS J<\\\/em>\\u00a0288, 4746-4772.\\u00a0<strong>IF 5.542<\\\/strong> DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1111\\\/febs.15830\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1111\\\/febs.15830<\\\/a><br><br>Peleg Y., Vincentelli R., Collins B. M., Chen K., Livingstone E. K., Weeratunga S., Leneva N., Guo Q., Remans K., Perez K., Bjerga G. E. K., Larsen \\u00d8., Van\\u011bk O., Sko\\u0159epa O., Jacquemin S., Poterszman A., Kjaer S., Christodoulou E., Albeck S., Dym O., Ainbinder E., Unger T., Schuetz A., Matthes S., Bader M., de Marco A., Storici P., Semrau M. S., Stolt-Bergner P., Aigner C., Suppmann S., Goldenzweig A., Fleishman S. J. (2021): Community-wide experimental evaluation of the PROSS stability-design method.\\u00a0<em>J Mol Biol<\\\/em>\\u00a0433, 166964.\\u00a0<strong>IF 6.151<\\\/strong> DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1016\\\/j.jmb.2021.166964\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1016\\\/j.jmb.2021.166964<\\\/a><br><br>L\\u00f3pez-M\\u00e9ndez B., Baron B., Brautigam C. A., Jowitt T. A., Knauer S. H., Uebel S., Williams M. A., Sedivy A., Abian O., Abreu C., Adamczyk M., Bal W., Berger S., Buell A. K., Carolis C., Daviter T., Fish A., Garcia-Alai M., Guenther C., Hamacek J., Holkov\\u00e1 J., Houser J., Johnson C., Kelly S., Leech A., Mas C., Matulis D., McLaughlin S. H., Montserret R., Nasreddine R., Nehm\\u00e9 R., Nguyen Q., Ortega-Alarc\\u00f3n D., Perez K., Pirc K., Piszczek G., Podobnik M., Rodrigo N., Rokov-Plavec J., Schaefer S., Sharpe T., Southall J., Staunton D., Tavares P., Van\\u011bk O., Weyand M., Wu D. (2021): Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study.\\u00a0<em>Eur Biophys J<\\\/em>\\u00a050, 411-427.\\u00a0<strong>IF 2.095<\\\/strong> DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1007\\\/s00249-021-01532-6\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1016\\\/10.1007\\\/s00249-021-01532-6<\\\/a><br><br>Bou\\u0161ov\\u00e1 K., Bedn\\u00e1rov\\u00e1 L., Zouharov\\u00e1 M., Vet\\u00fd\\u0161kov\\u00e1 V., Po\\u0161tulkov\\u00e1 K., Hofbauerov\\u00e1 K., Petrvalsk\\u00e1 O., Van\\u011bk O., Tripsianes K., Vondr\\u00e1\\u0161ek J. (2021): The order of PDZ3 and TrpCage in fusion chimeras determines their properties \\u2013 a biophysical characterization.\\u00a0<em>Protein Sci<\\\/em>\\u00a030, 1653-1666.\\u00a0<strong>IF 6.993<\\\/strong> DOI:\\u00a0<a href=\\\"https:\\\/\\\/doi.org\\\/10.1002\\\/pro.4107\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1002\\\/pro.4107<\\\/a><br><br>Kalouskov\\u00e1 B., Sko\\u0159epa O., Cmunt D., Abreu C., Krej\\u010dov\\u00e1 K., Bl\\u00e1ha J., Sieglov\\u00e1 I., Kr\\u00e1l V., F\\u00e1bry M., Pola R., Pechar M., Van\\u011bk O. (2021): Tumor marker B7-H6 bound to the coiled coil peptide-polymer conjugate enables targeted therapy by activating human natural killer cells.\\u00a0<em>Biomedicines<\\\/em>\\u00a09, 1597.\\u00a0<strong>IF 6.081<\\\/strong> DOI:\\u00a0<a href=\\\"https:\\\/\\\/doi.org\\\/10.3390\\\/biomedicines9111597\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.3390\\\/biomedicines9111597<\\\/a>\"}},{\"@type\":\"Question\",\"name\":\"2020\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Janisova L., Gruzinov A., Zaborova O. V., Velychkivska N., Van\\u011bk O., Chytil P., Etrych T., Janou\\u0161kov\\u00e1 O., Zhang X., Blanchet C., Papadakis C. M., Svergun D. I., Filippov S. K. (2020): Molecular mechanisms of the interactions of N-(2-hydroxypropyl)methacrylamide copolymers designed for cancer therapy with the blood plasma proteins.\\u00a0<em>Pharmaceutics<\\\/em>\\u00a012, 106.<strong>\\u00a0IF 6.321<\\\/strong> DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.3390\\\/pharmaceutics12020106\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.3390\\\/pharmaceutics12020106<\\\/a><br><br>Sko\\u0159epa O., Pa\\u017eick\\u00fd S., Kalouskov\\u00e1 B., Bl\\u00e1ha J., Abreu C., Je\\u010dmen T., Ros\\u016flek M., Fish A., Sedivy A., Harlos K., Dohn\\u00e1lek J., Sk\\u00e1lov\\u00e1 T., Van\\u011bk O. (2020): Natural killer cell activation receptor NKp30 oligomerization depends on its N-glycosylation.\\u00a0<em>Cancers<\\\/em>\\u00a012, 1998.\\u00a0<strong>IF 6.639<\\\/strong> DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.3390\\\/cancers12071998\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.3390\\\/cancers12071998<\\\/a><br><br>Sumov\\u00e1 P., Polansk\\u00e1 N., Le\\u0161tinov\\u00e1 T., Spitzov\\u00e1 T., Kalouskov\\u00e1 B., Van\\u011bk O., Volf P., Rohou\\u0161ov\\u00e1 I. (2020):\\u00a0<em>Phlebotomus perniciosus<\\\/em>\\u00a0recombinant salivary proteins polarize murine macrophages towards the anti-inflammatory phenotype.\\u00a0<em>Front Cell Infect Microbiol<\\\/em>\\u00a010, 427.\\u00a0<strong>IF 5.293\\u00a0 <\\\/strong>DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.3389\\\/fcimb.2020.00427\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.3389\\\/fcimb.2020.00427<\\\/a><br><br>Vet\\u00fd\\u0161kov\\u00e1 V., Zouharov\\u00e1 M., Bedn\\u00e1rov\\u00e1 L., Van\\u011bk O., S\\u00e1zelov\\u00e1 P., Ka\\u0161i\\u010dka V., Vym\\u011btal J., Srp J., Rumlov\\u00e1 M., Charnavets T., Po\\u0161tulkov\\u00e1 K., Reseland J. E., Bou\\u0161ov\\u00e1 K., Vondr\\u00e1\\u0161ek J. (2020): Characterization of AMBN I and II isoforms and study of their Ca2+-binding properties.\\u00a0<em>Int J Mol Sci<\\\/em>\\u00a021, 9293.\\u00a0<strong>IF 5.924 <\\\/strong>\\u00a0DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.3390\\\/ijms21239293\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.3390\\\/ijms21239293<\\\/a><br><br>Sk\\u00e1lov\\u00e1 T., Leng\\u00e1lov\\u00e1 A., Dohn\\u00e1lek J., Harlos K., Mihal\\u010din P., Kolenko P., Str\\u00e1\\u0148ava M., Bl\\u00e1ha J., Shimizu T., Mart\\u00ednkov\\u00e1 M. (2020): Disruption of the dimerization interface of the sensing domain in the dimeric heme-based oxygen sensor\\u00a0<em>Af<\\\/em>GcHK abolishes bacterial signal transduction.\\u00a0<em>J Biol Chem<\\\/em>\\u00a0295, 1587-1597.\\u00a0<strong>IF 5.157<\\\/strong>\\u00a0DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1074\\\/jbc.RA119.011574\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1074\\\/jbc.RA119.011574<\\\/a>\"}},{\"@type\":\"Question\",\"name\":\"2019\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Sumov\\u00e1 P., \\u0160\\u00edma M., Kalouskov\\u00e1 B., Polansk\\u00e1 N., Van\\u011bk O., Oliveira F., Valenzuela J. G., Volf P. (2019): Amine-binding properties of salivary yellow-related proteins in phlebotomine sand flies.\\u00a0<em>Insect Biochem Mol Biol<\\\/em>\\u00a0115, 103245.\\u00a0<strong>IF 3.827<\\\/strong>\\u00a0DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1016\\\/j.ibmb.2019.103245\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1016\\\/j.ibmb.2019.103245<\\\/a><br><br>Willen L., Le\\u0161tinov\\u00e1 T., Kalouskov\\u00e1 B., Sumov\\u00e1 P., Spitzov\\u00e1 T., Velez R., Domenech E., Van\\u011bk O., G\\u00e1llego M., Mertens P., Volf P. (2019): Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis.\\u00a0<em>PLoS Negl Trop Dis<\\\/em>\\u00a013, e0007832.\\u00a0<strong>IF 4.487<\\\/strong> DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1371\\\/journal.pntd.0007832\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1371\\\/journal.pntd.0007832<\\\/a><br><br>Van\\u011bk O., Celadov\\u00e1 P., Sko\\u0159epa O., Bl\\u00e1ha J., Kalouskov\\u00e1 B., Dvorsk\\u00e1 A., Pol\\u00e1chov\\u00e1 E., Pucholtov\\u00e1 H., Kavan D., Pompach P., Hofbauerov\\u00e1 K., Kopeck\\u00fd V. Jr., Mesci A., Voigt S., Carlyle J. R. (2019): Production of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells.\\u00a0<em>Sci Rep<\\\/em>\\u00a09, 17836.\\u00a0<strong>IF 4.011<\\\/strong>\\u00a0 DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1038\\\/s41598-019-52114-8\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1038\\\/s41598-019-52114-8<\\\/a>\"}},{\"@type\":\"Question\",\"name\":\"2018\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"\\u0160kerlov\\u00e1 J., Bl\\u00e1ha J., Pachl P., Hofbauerov\\u00e1 K., Kuka\\u010dka Z., Man P., Pompach P., Nov\\u00e1k P., Otwinowski Z., Brynda J., Van\\u011bk O., \\u0158ez\\u00e1\\u010dov\\u00e1 P. (2018): Crystal structure of native \\u03b2-N-acetylhexosaminidase isolated from\\u00a0<em>Aspergillus oryzae<\\\/em>\\u00a0sheds light onto its substrate specificity, high stability, and regulation by propeptide.\\u00a0<em>FEBS J<\\\/em>\\u00a0285, 580-598.\\u00a0<strong>IF 4.739<\\\/strong>\\u00a0DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1111\\\/febs.14360\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1111\\\/febs.14360<\\\/a><br><br>Mal\\u00fd M., Sk\\u00e1lov\\u00e1 T., \\u0160vecov\\u00e1 L., Dohn\\u00e1lek J., Bl\\u00e1ha J., Van\\u011bk O., Harlos K., Kolenko P. (2018): Paired refinement: Impact of reintegration and rescaling. In: Dragounov\\u00e1 K., ed.\\u00a0<em>Sborn\\u00edk 8. Studentsk\\u00e9 v\\u011bdeck\\u00e9 konference fyziky pevn\\u00fdch l\\u00e1tek a materi\\u00e1l\\u016f FJFI \\u010cVUT.<\\\/em>\\u00a0Praha: \\u010cesk\\u00e9 vysok\\u00e9 u\\u010den\\u00ed technick\\u00e9 v Praze, s. 8-10. ISBN 978-80-01-06511-2\"}},{\"@type\":\"Question\",\"name\":\"2017\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Bl\\u00e1ha J., Kalouskov\\u00e1 B., Sko\\u0159epa O., Pa\\u017eick\\u00fd S., Nov\\u00e1k P., Van\\u011bk O. (2017): High-level expression and purification of soluble form of human natural killer cell receptor NKR-P1 in HEK293S GnTI<sup>-<\\\/sup>\\u00a0cells.\\u00a0<em>Protein Expr Purif<\\\/em>\\u00a0140, 36-43.\\u00a0<strong>IF 1.351<\\\/strong>\\u00a0DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1016\\\/j.pep.2017.07.016\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1016\\\/j.pep.2017.07.016<\\\/a><br><br>Stehl\\u00edk \\u0160., Varga M., \\u0160tenclov\\u00e1 P., Ondi\\u010d L., Ledinsk\\u00fd M., Pangr\\u00e1c J., Van\\u011bk O., Lipov J., Kromka A., Rezek B. (2017): Ultrathin Nanocrystalline Diamond Films with Silicon Vacancy Color Centers via Seeding by 2 nm Detonation Nanodiamonds.\\u00a0<em>ACS Appl Mater Interfaces<\\\/em>\\u00a09, 38842-38853.\\u00a0<strong>IF 8.097<\\\/strong>\\u00a0 DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1021\\\/acsami.7b14436\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1021\\\/acsami.7b14436<\\\/a><br><br>Str\\u00e1\\u0148ava M., Man P., Sk\\u00e1lov\\u00e1 T., Kolenko P., Bl\\u00e1ha J., Fojt\\u00edkov\\u00e1 V., Mart\\u00ednek V., Dohn\\u00e1lek J., Leng\\u00e1lov\\u00e1 A., Ros\\u016flek M., Shimizu T., Mart\\u00ednkov\\u00e1 M. (2017): Coordination and redox state-dependent structural changes of the heme-based oxygen sensor\\u00a0<em>Af<\\\/em>GcHK associated with intraprotein signal transduction.\\u00a0<em>J Biol Chem<\\\/em>\\u00a0292, 20921-20935.\\u00a0<strong>IF 4.011<\\\/strong> DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1074\\\/jbc.M117.817023\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1074\\\/jbc.M117.817023<\\\/a>\"}},{\"@type\":\"Question\",\"name\":\"2016\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Dole\\u017eal M., Z\\u00e1bransk\\u00fd A., Dost\\u00e1l J., Van\\u011bk O., Brynda J., Lep\\u0161\\u00edk M., Hadravov\\u00e1 R., Pichov\\u00e1 I. (2016): Myristoylation drives dimerization of matrix protein from mouse mammary tumor virus.\\u00a0<em>Retrovirology<\\\/em>\\u00a013, 2.\\u00a0<strong>IF 4.185<\\\/strong>\\u00a0DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1186\\\/s12977-015-0235-8\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1186\\\/s12977-015-0235-8<\\\/a><br><br>Str\\u00e1\\u0148ava M., Mart\\u00ednek V., Man P., Fojt\\u00edkov\\u00e1 V., Kavan D., Van\\u011bk O., Shimizu T., Mart\\u00ednkov\\u00e1 M. (2016): Structural Characterization of the Heme-based Oxygen Sensor, AfGcHK, its Interactions with the Cognate Response Regulator, and their Combined Mechanism of Action in a Bacterial Two-component Signaling System.\\u00a0<em>Proteins<\\\/em>\\u00a084, 1375-1389.\\u00a0<strong>IF 2.499<\\\/strong>\\u00a0DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1002\\\/prot.25083\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1002\\\/prot.25083<\\\/a>\"}},{\"@type\":\"Question\",\"name\":\"2015\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Bl\\u00e1ha J., Pachl P., Nov\\u00e1k P., Van\\u011bk O. (2015): Expression and purification of soluble and stable ectodomain of natural killer cell receptor LLT1 through high-density transfection of suspension adapted HEK293S GnTI<sup>-<\\\/sup>\\u00a0cells.\\u00a0<em>Protein Expr Purif<\\\/em>\\u00a0109, 7-13.\\u00a0<strong>IF 1.695<\\\/strong>\\u00a0DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1016\\\/j.pep.2015.01.006\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1016\\\/j.pep.2015.01.006<\\\/a><br><br>Sk\\u00e1lov\\u00e1 T., Bl\\u00e1ha J., Harlos K., Du\\u0161kov\\u00e1 J., Kova\\u013e T., Str\\u00e1nsk\\u00fd J., Ha\\u0161ek J., Van\\u011bk O., Dohn\\u00e1lek J. (2015): Four crystal structures of human LLT1, a ligand for human NKR-P1, in varied glycosylation and oligomerization states.\\u00a0<em>Acta Crystallogr Sect D Biol Crystallogr<\\\/em>\\u00a071, 578-591.\\u00a0<strong>IF 2.680<\\\/strong> DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1107\\\/S1399004714027928\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1107\\\/S1399004714027928<\\\/a><br><br>Machov\\u00e1 I., Sn\\u00e1\\u0161el J., Dost\\u00e1l J., Brynda J., Fanfrl\\u00edk J., Singh M., Tar\\u00e1bek J., Van\\u011bk O., Bedn\\u00e1rov\\u00e1 L., Pichov\\u00e1 I. (2015): Structural and functional studies of phosphoenolpyruvate carboxykinase from Mycobacterium tuberculosis.\\u00a0<em>PLoS ONE<\\\/em>\\u00a010, e0120682.\\u00a0<strong>IF 3.534<\\\/strong>\\u00a0DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1371\\\/journal.pone.0120682\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1371\\\/journal.pone.0120682<\\\/a><br><br>Lynn G. M., Laga R., Darrah P. A., Ishizuka A. S., Balaci A. J., Dulcey A. E., Pechar M., Pola R., Gerner M. Y., Yamamoto A., Buechler C. R., Quinn K. M., Smelkinson M. G., Van\\u011bk O., Cawood R., Hills T., Vasalatiy O., Kastenm\\u00fcller K., Francica J. R., Stutts L., Tom J. K., Ryu K. A., Esser-Kahn A. P., Etrych T., Fisher K. D., Seymour L. W., Seder R. A. (2015):\\u00a0<em>In vivo<\\\/em>\\u00a0characterization of the physicochemical properties of polymer-linked TLR agonists that enhance vaccine immunogenicity.\\u00a0<em>Nat Biotechnol<\\\/em>\\u00a033, 1201-1210.\\u00a0<strong>IF 43.113<\\\/strong> \\u00a0DOI:\\u00a0<a href=\\\"http:\\\/\\\/dx.doi.org\\\/10.1038\\\/nbt.3371\\\" target=\\\"_blank\\\" rel=\\\"noreferrer noopener\\\">10.1038\\\/nbt.3371<\\\/a>\"}}]}<\/script><div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-6ec144a0 \" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\">\t\t\t<span class=\"uagb-icon uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M192 384c-8.188 0-16.38-3.125-22.62-9.375l-160-160c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0L192 306.8l137.4-137.4c12.5-12.5 32.75-12.5 45.25 0s12.5 32.75 0 45.25l-160 160C208.4 380.9 200.2 384 192 384z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"uagb-icon-active uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M352 352c-8.188 0-16.38-3.125-22.62-9.375L192 205.3l-137.4 137.4c-12.5 12.5-32.75 12.5-45.25 0s-12.5-32.75 0-45.25l160-160c12.5-12.5 32.75-12.5 45.25 0l160 160c12.5 12.5 12.5 32.75 0 45.25C368.4 348.9 360.2 352 352 352z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t<h4 class=\"uagb-question\">2026<\/h4><\/div><div class=\"uagb-faq-content\"><p>Kapisheva M., Junkov\u00e1 P., Van\u011bk O., Jalovcov\u00e1 Z., K\u0159\u00ed\u017eov\u00e1 I., Dost\u00e1lkov\u00e1 A., Rumlov\u00e1 M. (2026): Protease-mediated maturation of M-PMV reverse transcriptase into a functional heterodimer. Protein Sci 35, e70469. <strong>IF 5.2<\/strong> DOI: <a href=\"https:\/\/doi.org\/10.1002\/pro.70469\" data-type=\"link\" data-id=\"https:\/\/doi.org\/10.1002\/pro.70469\">10.1002\/pro.70469<\/a> <\/p><\/div><\/div><div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-a61a1106 \" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\">\t\t\t<span class=\"uagb-icon uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M192 384c-8.188 0-16.38-3.125-22.62-9.375l-160-160c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0L192 306.8l137.4-137.4c12.5-12.5 32.75-12.5 45.25 0s12.5 32.75 0 45.25l-160 160C208.4 380.9 200.2 384 192 384z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"uagb-icon-active uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M352 352c-8.188 0-16.38-3.125-22.62-9.375L192 205.3l-137.4 137.4c-12.5 12.5-32.75 12.5-45.25 0s-12.5-32.75 0-45.25l160-160c12.5-12.5 32.75-12.5 45.25 0l160 160c12.5 12.5 12.5 32.75 0 45.25C368.4 348.9 360.2 352 352 352z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t<h4 class=\"uagb-question\">2025<\/h4><\/div><div class=\"uagb-faq-content\"><p>Di Carluccio C., Nieto-Fabregat F., Cerofolini L., Abreu C., Padilla-Cort\u00e9s L., Gheorghita G. R., Masi A. A., Buono L., Ali M. G. A., Lamprinaki D., Molinaro A., Juge N., Smaldone G., Van\u011bk O., Fragai M., Marchetti R., Silipo A. (2025): <em>Fusobacterium nucleatum<\/em> LPS O-antigen defines a novel Siglec-7 binding epitope. JACS Au\u00a05, 5367-5380. <strong>IF 8.7<\/strong> DOI: <a href=\"https:\/\/doi.org\/10.1021\/jacsau.5c00810\" data-type=\"link\" data-id=\"https:\/\/doi.org\/10.1021\/jacsau.5c00810\">10.1021\/jacsau.5c00810<\/a> <br><br>La Gatta S., Leone L., Sgueglia G., \u0160imuni\u0107 L., Liao Y., Van\u011bk O., Chino M., Nastri F., Chiesa M., Lombardi A. (2025): Engineering a functional histidine brace copper-binding site into a <em>de novo<\/em>-designed protein scaffold. JACS Au 5, 4799-4810. <strong>IF 8.7<\/strong> DOI: <a href=\"https:\/\/doi.org\/10.1021\/jacsau.5c00754\" data-type=\"link\" data-id=\"https:\/\/doi.org\/10.1021\/jacsau.5c00754\">10.1021\/jacsau.5c00754<\/a><br><br>Pachmayr I., Masullo L. A., Reinhardt S. C. M., Kwon J., Llop M., Sko\u0159epa O., Herter S., Bacac M., Klein C., Jungmann R. (2025): Resolving the structural basis of therapeutic antibody function in cancer immunotherapy with RESI. Nat Commun 16, 6768. <strong>IF 15.7<\/strong> DOI: <a href=\"https:\/\/doi.org\/10.1038\/s41467-025-61893-w\" data-type=\"link\" data-id=\"https:\/\/doi.org\/10.1038\/s41467-025-61893-w\">10.1038\/s41467-025-61893-w<\/a><br><br>Di Carluccio C., Padilla-Cort\u00e9s L., Tiemblo-Mart\u00ecn M., Gheorghita G. R., Oliva R., Cerofolini L., Masi A. A., Abreu C., Tseng H. K., Molinaro A., Del Vecchio P., Van\u011bk O., Lin C. C., Marchetti R., Fragai M., Silipo A. (2025): Insights into Siglec-7 binding to gangliosides: NMR protein assignment and the impact of ligand flexibility. Adv Sci 12, e2415782. <strong>IF 14.1<\/strong> DOI: <a href=\"https:\/\/doi.org\/10.1002\/advs.202415782\">10.1002\/advs.202415782<\/a><br><br>Di Carluccio C., Gerpe Amor T., Lenza M. P., Masi A. A., Abreu C., Longo V., Albano F., Nieto-Fabregat F., Salvatore P., Falco G., Santana-Medero D., Fragai M., van Kooyk Y., Molinaro A., Valdes-Balbin Y.,\u00a0Van\u011bk\u00a0O., Verez-Bencomo V., Marchetti R., Chiodo F., Silipo A. (2025): Molecular basis of Siglec-7 recognition by\u00a0<em>Neisseria meningitidis<\/em>\u00a0serogroup Y CPS: Implications for immune evasion.\u00a0<em>JACS Au<\/em>\u00a05, 2257-2269.\u00a0<strong>IF 8.7<\/strong>\u00a0DOI:\u00a0<a href=\"https:\/\/doi.org\/10.1021\/jacsau.5c00214\" target=\"_blank\" rel=\"noreferrer noopener\">10.1021\/jacsau.5c00214<\/a><br><br>Abreu C., Di Carluccio C., Je\u010dmen T., Sko\u0159epa O., Bl\u00e1ha J., Marchetti R., Silipo A., Van\u011bk O. (2025): Insights into stability, dimerisation, and ligand binding properties of Siglec-7: Isotope labelling in HEK293 cells for protein characterisation by NMR spectroscopy. <em>Int J Biol Macromol <\/em>309, 142672. <strong>IF 8.5<\/strong> DOI: <a href=\"https:\/\/doi.org\/10.1016\/j.ijbiomac.2025.142672\" target=\"_blank\" rel=\"noreferrer noopener\">10.1016\/j.ijbiomac.2025.142672<\/a><br><br>Nejadebrahim S., Houserov\u00e1 J., Je\u010dmen T., Kalouskov\u00e1 B., Abreu C., Herynek \u0160., Sko\u0159epa O., Bl\u00e1ha J., Van\u011bk O. (2025): Multiple O- and an N-glycosylation of the stalk region of the NK cell activation receptor NKp46 mediates its interaction with the <em>Candida glabrata<\/em> epithelial adhesin 1. <em>Int J Biol Macromol<\/em> 310, 143037. <strong>IF 8.5<\/strong> DOI: <a class=\"\" href=\"https:\/\/doi.org\/10.1016\/j.ijbiomac.2025.143037\">10.1016\/j.ijbiomac.2025.143037<\/a><\/p><\/div><\/div><div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-2734ac86 \" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\">\t\t\t<span class=\"uagb-icon uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M192 384c-8.188 0-16.38-3.125-22.62-9.375l-160-160c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0L192 306.8l137.4-137.4c12.5-12.5 32.75-12.5 45.25 0s12.5 32.75 0 45.25l-160 160C208.4 380.9 200.2 384 192 384z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"uagb-icon-active uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M352 352c-8.188 0-16.38-3.125-22.62-9.375L192 205.3l-137.4 137.4c-12.5 12.5-32.75 12.5-45.25 0s-12.5-32.75 0-45.25l160-160c12.5-12.5 32.75-12.5 45.25 0l160 160c12.5 12.5 12.5 32.75 0 45.25C368.4 348.9 360.2 352 352 352z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t<h4 class=\"uagb-question\">2024<\/h4><\/div><div class=\"uagb-faq-content\"><p>Leonard E. K., Tomala J., Gould J. R., Leff M. I., Lin J. X., Li P., Porter M. J., Johansen E. R., Thompson L., Cao S. D., Hou S., Henclov\u00e1 T., Huli\u010diak M., Sargunas P. R., Fabilane C. S., Van\u011bk O., Kov\u00e1\u0159 M., Schneider B., Raimondi G., Leonard W. J., Spangler J. B. (2024): Engineered cytokine\/antibody fusion proteins improve IL-2 delivery to pro-inflammatory cells and promote antitumor activity. <em>JCI Insight<\/em> 9, e173469. <strong>IF 6.3<\/strong> DOI: <a href=\"http:\/\/dx.doi.org\/10.1172\/jci.insight.173469\">10.1172\/jci.insight.173469<\/a><\/p><\/div><\/div><div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-ab253c25 \" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\">\t\t\t<span class=\"uagb-icon uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M192 384c-8.188 0-16.38-3.125-22.62-9.375l-160-160c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0L192 306.8l137.4-137.4c12.5-12.5 32.75-12.5 45.25 0s12.5 32.75 0 45.25l-160 160C208.4 380.9 200.2 384 192 384z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"uagb-icon-active uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M352 352c-8.188 0-16.38-3.125-22.62-9.375L192 205.3l-137.4 137.4c-12.5 12.5-32.75 12.5-45.25 0s-12.5-32.75 0-45.25l160-160c12.5-12.5 32.75-12.5 45.25 0l160 160c12.5 12.5 12.5 32.75 0 45.25C368.4 348.9 360.2 352 352 352z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t<h4 class=\"uagb-question\">2023<\/h4><\/div><div class=\"uagb-faq-content\"><p>Di Carluccio C., Milanesi F., Civera M., Abreu C., Sattin S., Francesconi O., Molinaro A., Van\u011bk O., Marchetti R., Silipo A. (2023): Tumor carbohydrate associated antigen analogs as potential binders for Siglec-7. <em>Eur J Org Chem<\/em> 26, e202300644. <strong>IF 2.8<\/strong> DOI: <a href=\"https:\/\/doi.org\/10.1002\/ejoc.202300644\">10.1002\/ejoc.202300644<\/a><\/p><\/div><\/div><div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-d47a7172 \" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\">\t\t\t<span class=\"uagb-icon uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M192 384c-8.188 0-16.38-3.125-22.62-9.375l-160-160c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0L192 306.8l137.4-137.4c12.5-12.5 32.75-12.5 45.25 0s12.5 32.75 0 45.25l-160 160C208.4 380.9 200.2 384 192 384z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"uagb-icon-active uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M352 352c-8.188 0-16.38-3.125-22.62-9.375L192 205.3l-137.4 137.4c-12.5 12.5-32.75 12.5-45.25 0s-12.5-32.75 0-45.25l160-160c12.5-12.5 32.75-12.5 45.25 0l160 160c12.5 12.5 12.5 32.75 0 45.25C368.4 348.9 360.2 352 352 352z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t<h4 class=\"uagb-question\">2022<\/h4><\/div><div class=\"uagb-faq-content\"><p>Anderluh M., Berti F., Bzducha-Wr\u00f3bel A., Chiodo F., Colombo C., Compostella F., Durlik K., Ferhati X., Holmdahl R., Jovanovic D., Kaca W., Lay L., Marinovic-Cincovic M., Marradi M., Ozil M., Polito L., Reina-Martin J. J., Reis C. A., Sackstein R., Silipo A., \u0160vajger U., Van\u011bk O., Yamamoto F., Richichi B., van Vliet S. J. (2022): Recent advances on smart glycoconjugate vaccines in infections and cancer.\u00a0<em>FEBS J<\/em>\u00a0289, 4251\u20134303.\u00a0<strong>IF 5.622<\/strong> DOI:\u00a0<a rel=\"noreferrer noopener\" href=\"http:\/\/dx.doi.org\/10.1111\/febs.15909\" target=\"_blank\">10.1111\/febs.15909<\/a><br><br>Van\u011bk O., Kalouskov\u00e1 B., Abreu C., Nejadebrahim S., Sko\u0159epa O. (2022): Natural killer cell-based strategies for immunotherapy of cancer.\u00a0<em>Adv Protein Chem Struct Biol<\/em>\u00a0129, 91-133.\u00a0<strong>IF 5.447<\/strong> DOI:\u00a0<a rel=\"noreferrer noopener\" href=\"https:\/\/doi.org\/10.1016\/bs.apcsb.2022.02.001\" target=\"_blank\">10.1016\/bs.apcsb.2022.02.001<\/a><br><br>Remans K., Lebendiker M., Abreu C., Maffei M., Sellathurai S., May M. M., Van\u011bk O., de Marco A. (2022): Protein purification strategies must consider downstream applications and individual biological characteristics.\u00a0<em>Microb Cell Fact<\/em>\u00a021, 52.\u00a0<strong>IF 6.352<\/strong> DOI:\u00a0<a rel=\"noreferrer noopener\" href=\"https:\/\/doi.org\/10.1186\/s12934-022-01778-5\" target=\"_blank\">10.1186\/s12934-022-01778-5<\/a><br><br>Bl\u00e1ha J., Sk\u00e1lov\u00e1 T., Kalouskov\u00e1 B., Sko\u0159epa O., Cmunt D., Grob\u00e1rov\u00e1 V., Pazicky S., Pol\u00e1chov\u00e1 E., Abreu C., Str\u00e1nsk\u00fd J., Kova\u013e T., Du\u0161kov\u00e1 J., Zhao Y., Harlos K., Ha\u0161ek J., Dohn\u00e1lek J., Van\u011bk O. (2022): Structure of the human NK cell NKR-P1:LLT1 receptor:ligand complex reveals clustering in the immune synapse.\u00a0<em>Nat Commun<\/em>\u00a013, 5022.\u00a0<strong>IF 17.694<\/strong> DOI:\u00a0<a rel=\"noreferrer noopener\" href=\"https:\/\/doi.org\/10.1038\/s41467-022-32577-6\" target=\"_blank\">10.1038\/s41467-022-32577-6<\/a><\/p><\/div><\/div><div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-849c71d1 \" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\">\t\t\t<span class=\"uagb-icon uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M192 384c-8.188 0-16.38-3.125-22.62-9.375l-160-160c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0L192 306.8l137.4-137.4c12.5-12.5 32.75-12.5 45.25 0s12.5 32.75 0 45.25l-160 160C208.4 380.9 200.2 384 192 384z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"uagb-icon-active uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M352 352c-8.188 0-16.38-3.125-22.62-9.375L192 205.3l-137.4 137.4c-12.5 12.5-32.75 12.5-45.25 0s-12.5-32.75 0-45.25l160-160c12.5-12.5 32.75-12.5 45.25 0l160 160c12.5 12.5 12.5 32.75 0 45.25C368.4 348.9 360.2 352 352 352z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t<h4 class=\"uagb-question\">2021<\/h4><\/div><div class=\"uagb-faq-content\"><p>Stehl\u00edk \u0160., Henych J., \u0160tenclov\u00e1 P., Kr\u00e1l R., Zemenov\u00e1 P., Pangr\u00e1c J., Van\u011bk O., Kromka A., Rezek B. (2021): Size and nitrogen inhomogeneity in detonation and laser synthesized primary nanodiamond particles revealed via salt-assisted deaggregation.\u00a0<em>Carbon<\/em>\u00a0171, 230-239.\u00a0<strong>IF 11.307<\/strong> DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1016\/j.carbon.2020.09.026\" target=\"_blank\" rel=\"noreferrer noopener\">10.1016\/j.carbon.2020.09.026<\/a><br><br>Zouharov\u00e1 M., Vym\u011btal J., Bedn\u00e1rov\u00e1 L., Van\u011bk O., Herman P., Vet\u00fd\u0161kov\u00e1 V., Po\u0161tulkov\u00e1 K., Lingstaadas P. S., Vondr\u00e1\u0161ek J., Bou\u0161ov\u00e1 K. (2021): Intrinsically disordered protein domain of human ameloblastin in synthetic fusion with calmodulin increases calmodulin stability and modulates its function.\u00a0<em>Int J Biol Macromol<\/em>\u00a0168, 1-12.\u00a0<strong>IF 8.025<\/strong> DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1016\/j.ijbiomac.2020.11.216\" target=\"_blank\" rel=\"noreferrer noopener\">10.1016\/j.ijbiomac.2020.11.216<\/a><br><br>Stehl\u00edk \u0160., Mermoux M., Schummer B., Van\u011bk O., Kol\u00e1\u0159ov\u00e1 K., \u0160tenclov\u00e1 P., Vlk A., Ledinsk\u00fd M., Pfeifer R., Romanyuk O., Gordeev I., Roussel-Dherbey F., N\u011bme\u010dkov\u00e1 Z., Henych J., Bezdi\u010dka P., Kromka A., Rezek B. (2021): Size effects on surface chemistry and Raman spectra of sub-5 nm oxidized high-pressure high-temperature and detonation nanodiamonds.\u00a0<em>J Phys Chem C<\/em>\u00a0125, 5647-5669.\u00a0<strong>IF 4.189<\/strong> DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1021\/acs.jpcc.0c09190\" target=\"_blank\" rel=\"noreferrer noopener\">10.1021\/acs.jpcc.0c09190<\/a><br><br>Anderluh M., Berti F., Bzducha-Wr\u00f3bel A., Chiodo F., Colombo C., Compostella F., Durlik K., Ferhati X., Holmdahl R., Jovanovic D., Kaca W., Lay L., Marinovic-Cincovic M., Marradi M., Ozil M., Polito L., Reina-Martin J. J., Reis C. A., Sackstein R., Silipo A., \u0160vajger U., Van\u011bk O., Yamamoto F., Richichi B., van Vliet S. J. (2021): Emerging glyco-based strategies to steer immune responses.\u00a0<em>FEBS J<\/em>\u00a0288, 4746-4772.\u00a0<strong>IF 5.542<\/strong> DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1111\/febs.15830\" target=\"_blank\" rel=\"noreferrer noopener\">10.1111\/febs.15830<\/a><br><br>Peleg Y., Vincentelli R., Collins B. M., Chen K., Livingstone E. K., Weeratunga S., Leneva N., Guo Q., Remans K., Perez K., Bjerga G. E. K., Larsen \u00d8., Van\u011bk O., Sko\u0159epa O., Jacquemin S., Poterszman A., Kjaer S., Christodoulou E., Albeck S., Dym O., Ainbinder E., Unger T., Schuetz A., Matthes S., Bader M., de Marco A., Storici P., Semrau M. S., Stolt-Bergner P., Aigner C., Suppmann S., Goldenzweig A., Fleishman S. J. (2021): Community-wide experimental evaluation of the PROSS stability-design method.\u00a0<em>J Mol Biol<\/em>\u00a0433, 166964.\u00a0<strong>IF 6.151<\/strong> DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1016\/j.jmb.2021.166964\" target=\"_blank\" rel=\"noreferrer noopener\">10.1016\/j.jmb.2021.166964<\/a><br><br>L\u00f3pez-M\u00e9ndez B., Baron B., Brautigam C. A., Jowitt T. A., Knauer S. H., Uebel S., Williams M. A., Sedivy A., Abian O., Abreu C., Adamczyk M., Bal W., Berger S., Buell A. K., Carolis C., Daviter T., Fish A., Garcia-Alai M., Guenther C., Hamacek J., Holkov\u00e1 J., Houser J., Johnson C., Kelly S., Leech A., Mas C., Matulis D., McLaughlin S. H., Montserret R., Nasreddine R., Nehm\u00e9 R., Nguyen Q., Ortega-Alarc\u00f3n D., Perez K., Pirc K., Piszczek G., Podobnik M., Rodrigo N., Rokov-Plavec J., Schaefer S., Sharpe T., Southall J., Staunton D., Tavares P., Van\u011bk O., Weyand M., Wu D. (2021): Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study.\u00a0<em>Eur Biophys J<\/em>\u00a050, 411-427.\u00a0<strong>IF 2.095<\/strong> DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1007\/s00249-021-01532-6\" target=\"_blank\" rel=\"noreferrer noopener\">10.1016\/10.1007\/s00249-021-01532-6<\/a><br><br>Bou\u0161ov\u00e1 K., Bedn\u00e1rov\u00e1 L., Zouharov\u00e1 M., Vet\u00fd\u0161kov\u00e1 V., Po\u0161tulkov\u00e1 K., Hofbauerov\u00e1 K., Petrvalsk\u00e1 O., Van\u011bk O., Tripsianes K., Vondr\u00e1\u0161ek J. (2021): The order of PDZ3 and TrpCage in fusion chimeras determines their properties \u2013 a biophysical characterization.\u00a0<em>Protein Sci<\/em>\u00a030, 1653-1666.\u00a0<strong>IF 6.993<\/strong> DOI:\u00a0<a href=\"https:\/\/doi.org\/10.1002\/pro.4107\" target=\"_blank\" rel=\"noreferrer noopener\">10.1002\/pro.4107<\/a><br><br>Kalouskov\u00e1 B., Sko\u0159epa O., Cmunt D., Abreu C., Krej\u010dov\u00e1 K., Bl\u00e1ha J., Sieglov\u00e1 I., Kr\u00e1l V., F\u00e1bry M., Pola R., Pechar M., Van\u011bk O. (2021): Tumor marker B7-H6 bound to the coiled coil peptide-polymer conjugate enables targeted therapy by activating human natural killer cells.\u00a0<em>Biomedicines<\/em>\u00a09, 1597.\u00a0<strong>IF 6.081<\/strong> DOI:\u00a0<a href=\"https:\/\/doi.org\/10.3390\/biomedicines9111597\" target=\"_blank\" rel=\"noreferrer noopener\">10.3390\/biomedicines9111597<\/a><\/p><\/div><\/div><div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-61664fd5 \" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\">\t\t\t<span class=\"uagb-icon uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M192 384c-8.188 0-16.38-3.125-22.62-9.375l-160-160c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0L192 306.8l137.4-137.4c12.5-12.5 32.75-12.5 45.25 0s12.5 32.75 0 45.25l-160 160C208.4 380.9 200.2 384 192 384z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"uagb-icon-active uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M352 352c-8.188 0-16.38-3.125-22.62-9.375L192 205.3l-137.4 137.4c-12.5 12.5-32.75 12.5-45.25 0s-12.5-32.75 0-45.25l160-160c12.5-12.5 32.75-12.5 45.25 0l160 160c12.5 12.5 12.5 32.75 0 45.25C368.4 348.9 360.2 352 352 352z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t<h4 class=\"uagb-question\">2020<\/h4><\/div><div class=\"uagb-faq-content\"><p>Janisova L., Gruzinov A., Zaborova O. V., Velychkivska N., Van\u011bk O., Chytil P., Etrych T., Janou\u0161kov\u00e1 O., Zhang X., Blanchet C., Papadakis C. M., Svergun D. I., Filippov S. K. (2020): Molecular mechanisms of the interactions of N-(2-hydroxypropyl)methacrylamide copolymers designed for cancer therapy with the blood plasma proteins.\u00a0<em>Pharmaceutics<\/em>\u00a012, 106.<strong>\u00a0IF 6.321<\/strong> DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.3390\/pharmaceutics12020106\" target=\"_blank\" rel=\"noreferrer noopener\">10.3390\/pharmaceutics12020106<\/a><br><br>Sko\u0159epa O., Pa\u017eick\u00fd S., Kalouskov\u00e1 B., Bl\u00e1ha J., Abreu C., Je\u010dmen T., Ros\u016flek M., Fish A., Sedivy A., Harlos K., Dohn\u00e1lek J., Sk\u00e1lov\u00e1 T., Van\u011bk O. (2020): Natural killer cell activation receptor NKp30 oligomerization depends on its N-glycosylation.\u00a0<em>Cancers<\/em>\u00a012, 1998.\u00a0<strong>IF 6.639<\/strong> DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.3390\/cancers12071998\" target=\"_blank\" rel=\"noreferrer noopener\">10.3390\/cancers12071998<\/a><br><br>Sumov\u00e1 P., Polansk\u00e1 N., Le\u0161tinov\u00e1 T., Spitzov\u00e1 T., Kalouskov\u00e1 B., Van\u011bk O., Volf P., Rohou\u0161ov\u00e1 I. (2020):\u00a0<em>Phlebotomus perniciosus<\/em>\u00a0recombinant salivary proteins polarize murine macrophages towards the anti-inflammatory phenotype.\u00a0<em>Front Cell Infect Microbiol<\/em>\u00a010, 427.\u00a0<strong>IF 5.293\u00a0 <\/strong>DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.3389\/fcimb.2020.00427\" target=\"_blank\" rel=\"noreferrer noopener\">10.3389\/fcimb.2020.00427<\/a><br><br>Vet\u00fd\u0161kov\u00e1 V., Zouharov\u00e1 M., Bedn\u00e1rov\u00e1 L., Van\u011bk O., S\u00e1zelov\u00e1 P., Ka\u0161i\u010dka V., Vym\u011btal J., Srp J., Rumlov\u00e1 M., Charnavets T., Po\u0161tulkov\u00e1 K., Reseland J. E., Bou\u0161ov\u00e1 K., Vondr\u00e1\u0161ek J. (2020): Characterization of AMBN I and II isoforms and study of their Ca2+-binding properties.\u00a0<em>Int J Mol Sci<\/em>\u00a021, 9293.\u00a0<strong>IF 5.924 <\/strong>\u00a0DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.3390\/ijms21239293\" target=\"_blank\" rel=\"noreferrer noopener\">10.3390\/ijms21239293<\/a><br><br>Sk\u00e1lov\u00e1 T., Leng\u00e1lov\u00e1 A., Dohn\u00e1lek J., Harlos K., Mihal\u010din P., Kolenko P., Str\u00e1\u0148ava M., Bl\u00e1ha J., Shimizu T., Mart\u00ednkov\u00e1 M. (2020): Disruption of the dimerization interface of the sensing domain in the dimeric heme-based oxygen sensor\u00a0<em>Af<\/em>GcHK abolishes bacterial signal transduction.\u00a0<em>J Biol Chem<\/em>\u00a0295, 1587-1597.\u00a0<strong>IF 5.157<\/strong>\u00a0DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1074\/jbc.RA119.011574\" target=\"_blank\" rel=\"noreferrer noopener\">10.1074\/jbc.RA119.011574<\/a><\/p><\/div><\/div><div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-be3aeb02 \" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\">\t\t\t<span class=\"uagb-icon uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M192 384c-8.188 0-16.38-3.125-22.62-9.375l-160-160c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0L192 306.8l137.4-137.4c12.5-12.5 32.75-12.5 45.25 0s12.5 32.75 0 45.25l-160 160C208.4 380.9 200.2 384 192 384z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"uagb-icon-active uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M352 352c-8.188 0-16.38-3.125-22.62-9.375L192 205.3l-137.4 137.4c-12.5 12.5-32.75 12.5-45.25 0s-12.5-32.75 0-45.25l160-160c12.5-12.5 32.75-12.5 45.25 0l160 160c12.5 12.5 12.5 32.75 0 45.25C368.4 348.9 360.2 352 352 352z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t<h4 class=\"uagb-question\">2019<\/h4><\/div><div class=\"uagb-faq-content\"><p>Sumov\u00e1 P., \u0160\u00edma M., Kalouskov\u00e1 B., Polansk\u00e1 N., Van\u011bk O., Oliveira F., Valenzuela J. G., Volf P. (2019): Amine-binding properties of salivary yellow-related proteins in phlebotomine sand flies.\u00a0<em>Insect Biochem Mol Biol<\/em>\u00a0115, 103245.\u00a0<strong>IF 3.827<\/strong>\u00a0DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1016\/j.ibmb.2019.103245\" target=\"_blank\" rel=\"noreferrer noopener\">10.1016\/j.ibmb.2019.103245<\/a><br><br>Willen L., Le\u0161tinov\u00e1 T., Kalouskov\u00e1 B., Sumov\u00e1 P., Spitzov\u00e1 T., Velez R., Domenech E., Van\u011bk O., G\u00e1llego M., Mertens P., Volf P. (2019): Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis.\u00a0<em>PLoS Negl Trop Dis<\/em>\u00a013, e0007832.\u00a0<strong>IF 4.487<\/strong> DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1371\/journal.pntd.0007832\" target=\"_blank\" rel=\"noreferrer noopener\">10.1371\/journal.pntd.0007832<\/a><br><br>Van\u011bk O., Celadov\u00e1 P., Sko\u0159epa O., Bl\u00e1ha J., Kalouskov\u00e1 B., Dvorsk\u00e1 A., Pol\u00e1chov\u00e1 E., Pucholtov\u00e1 H., Kavan D., Pompach P., Hofbauerov\u00e1 K., Kopeck\u00fd V. Jr., Mesci A., Voigt S., Carlyle J. R. (2019): Production of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells.\u00a0<em>Sci Rep<\/em>\u00a09, 17836.\u00a0<strong>IF 4.011<\/strong>\u00a0 DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1038\/s41598-019-52114-8\" target=\"_blank\" rel=\"noreferrer noopener\">10.1038\/s41598-019-52114-8<\/a><\/p><\/div><\/div><div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-78cc7a67 \" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\">\t\t\t<span class=\"uagb-icon uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M192 384c-8.188 0-16.38-3.125-22.62-9.375l-160-160c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0L192 306.8l137.4-137.4c12.5-12.5 32.75-12.5 45.25 0s12.5 32.75 0 45.25l-160 160C208.4 380.9 200.2 384 192 384z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"uagb-icon-active uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M352 352c-8.188 0-16.38-3.125-22.62-9.375L192 205.3l-137.4 137.4c-12.5 12.5-32.75 12.5-45.25 0s-12.5-32.75 0-45.25l160-160c12.5-12.5 32.75-12.5 45.25 0l160 160c12.5 12.5 12.5 32.75 0 45.25C368.4 348.9 360.2 352 352 352z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t<h4 class=\"uagb-question\">2018<\/h4><\/div><div class=\"uagb-faq-content\"><p>\u0160kerlov\u00e1 J., Bl\u00e1ha J., Pachl P., Hofbauerov\u00e1 K., Kuka\u010dka Z., Man P., Pompach P., Nov\u00e1k P., Otwinowski Z., Brynda J., Van\u011bk O., \u0158ez\u00e1\u010dov\u00e1 P. (2018): Crystal structure of native \u03b2-N-acetylhexosaminidase isolated from\u00a0<em>Aspergillus oryzae<\/em>\u00a0sheds light onto its substrate specificity, high stability, and regulation by propeptide.\u00a0<em>FEBS J<\/em>\u00a0285, 580-598.\u00a0<strong>IF 4.739<\/strong>\u00a0DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1111\/febs.14360\" target=\"_blank\" rel=\"noreferrer noopener\">10.1111\/febs.14360<\/a><br><br>Mal\u00fd M., Sk\u00e1lov\u00e1 T., \u0160vecov\u00e1 L., Dohn\u00e1lek J., Bl\u00e1ha J., Van\u011bk O., Harlos K., Kolenko P. (2018): Paired refinement: Impact of reintegration and rescaling. In: Dragounov\u00e1 K., ed.\u00a0<em>Sborn\u00edk 8. Studentsk\u00e9 v\u011bdeck\u00e9 konference fyziky pevn\u00fdch l\u00e1tek a materi\u00e1l\u016f FJFI \u010cVUT.<\/em>\u00a0Praha: \u010cesk\u00e9 vysok\u00e9 u\u010den\u00ed technick\u00e9 v Praze, s. 8-10. ISBN 978-80-01-06511-2<\/p><\/div><\/div><div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-e34a6d1b \" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\">\t\t\t<span class=\"uagb-icon uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M192 384c-8.188 0-16.38-3.125-22.62-9.375l-160-160c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0L192 306.8l137.4-137.4c12.5-12.5 32.75-12.5 45.25 0s12.5 32.75 0 45.25l-160 160C208.4 380.9 200.2 384 192 384z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"uagb-icon-active uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M352 352c-8.188 0-16.38-3.125-22.62-9.375L192 205.3l-137.4 137.4c-12.5 12.5-32.75 12.5-45.25 0s-12.5-32.75 0-45.25l160-160c12.5-12.5 32.75-12.5 45.25 0l160 160c12.5 12.5 12.5 32.75 0 45.25C368.4 348.9 360.2 352 352 352z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t<h4 class=\"uagb-question\">2017<\/h4><\/div><div class=\"uagb-faq-content\"><p>Bl\u00e1ha J., Kalouskov\u00e1 B., Sko\u0159epa O., Pa\u017eick\u00fd S., Nov\u00e1k P., Van\u011bk O. (2017): High-level expression and purification of soluble form of human natural killer cell receptor NKR-P1 in HEK293S GnTI<sup>&#8211;<\/sup>\u00a0cells.\u00a0<em>Protein Expr Purif<\/em>\u00a0140, 36-43.\u00a0<strong>IF 1.351<\/strong>\u00a0DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1016\/j.pep.2017.07.016\" target=\"_blank\" rel=\"noreferrer noopener\">10.1016\/j.pep.2017.07.016<\/a><br><br>Stehl\u00edk \u0160., Varga M., \u0160tenclov\u00e1 P., Ondi\u010d L., Ledinsk\u00fd M., Pangr\u00e1c J., Van\u011bk O., Lipov J., Kromka A., Rezek B. (2017): Ultrathin Nanocrystalline Diamond Films with Silicon Vacancy Color Centers via Seeding by 2 nm Detonation Nanodiamonds.\u00a0<em>ACS Appl Mater Interfaces<\/em>\u00a09, 38842-38853.\u00a0<strong>IF 8.097<\/strong>\u00a0 DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1021\/acsami.7b14436\" target=\"_blank\" rel=\"noreferrer noopener\">10.1021\/acsami.7b14436<\/a><br><br>Str\u00e1\u0148ava M., Man P., Sk\u00e1lov\u00e1 T., Kolenko P., Bl\u00e1ha J., Fojt\u00edkov\u00e1 V., Mart\u00ednek V., Dohn\u00e1lek J., Leng\u00e1lov\u00e1 A., Ros\u016flek M., Shimizu T., Mart\u00ednkov\u00e1 M. (2017): Coordination and redox state-dependent structural changes of the heme-based oxygen sensor\u00a0<em>Af<\/em>GcHK associated with intraprotein signal transduction.\u00a0<em>J Biol Chem<\/em>\u00a0292, 20921-20935.\u00a0<strong>IF 4.011<\/strong> DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1074\/jbc.M117.817023\" target=\"_blank\" rel=\"noreferrer noopener\">10.1074\/jbc.M117.817023<\/a><\/p><\/div><\/div><div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-daad9095 \" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\">\t\t\t<span class=\"uagb-icon uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M192 384c-8.188 0-16.38-3.125-22.62-9.375l-160-160c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0L192 306.8l137.4-137.4c12.5-12.5 32.75-12.5 45.25 0s12.5 32.75 0 45.25l-160 160C208.4 380.9 200.2 384 192 384z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"uagb-icon-active uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M352 352c-8.188 0-16.38-3.125-22.62-9.375L192 205.3l-137.4 137.4c-12.5 12.5-32.75 12.5-45.25 0s-12.5-32.75 0-45.25l160-160c12.5-12.5 32.75-12.5 45.25 0l160 160c12.5 12.5 12.5 32.75 0 45.25C368.4 348.9 360.2 352 352 352z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t<h4 class=\"uagb-question\">2016<\/h4><\/div><div class=\"uagb-faq-content\"><p>Dole\u017eal M., Z\u00e1bransk\u00fd A., Dost\u00e1l J., Van\u011bk O., Brynda J., Lep\u0161\u00edk M., Hadravov\u00e1 R., Pichov\u00e1 I. (2016): Myristoylation drives dimerization of matrix protein from mouse mammary tumor virus.\u00a0<em>Retrovirology<\/em>\u00a013, 2.\u00a0<strong>IF 4.185<\/strong>\u00a0DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1186\/s12977-015-0235-8\" target=\"_blank\" rel=\"noreferrer noopener\">10.1186\/s12977-015-0235-8<\/a><br><br>Str\u00e1\u0148ava M., Mart\u00ednek V., Man P., Fojt\u00edkov\u00e1 V., Kavan D., Van\u011bk O., Shimizu T., Mart\u00ednkov\u00e1 M. (2016): Structural Characterization of the Heme-based Oxygen Sensor, AfGcHK, its Interactions with the Cognate Response Regulator, and their Combined Mechanism of Action in a Bacterial Two-component Signaling System.\u00a0<em>Proteins<\/em>\u00a084, 1375-1389.\u00a0<strong>IF 2.499<\/strong>\u00a0DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1002\/prot.25083\" target=\"_blank\" rel=\"noreferrer noopener\">10.1002\/prot.25083<\/a><\/p><\/div><\/div><div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-0c227421 \" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\">\t\t\t<span class=\"uagb-icon uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M192 384c-8.188 0-16.38-3.125-22.62-9.375l-160-160c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0L192 306.8l137.4-137.4c12.5-12.5 32.75-12.5 45.25 0s12.5 32.75 0 45.25l-160 160C208.4 380.9 200.2 384 192 384z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"uagb-icon-active uagb-faq-icon-wrap\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox= \"0 0 384 512\"><path d=\"M352 352c-8.188 0-16.38-3.125-22.62-9.375L192 205.3l-137.4 137.4c-12.5 12.5-32.75 12.5-45.25 0s-12.5-32.75 0-45.25l160-160c12.5-12.5 32.75-12.5 45.25 0l160 160c12.5 12.5 12.5 32.75 0 45.25C368.4 348.9 360.2 352 352 352z\"><\/path><\/svg>\n\t\t\t\t\t\t\t<\/span>\n\t\t\t<h4 class=\"uagb-question\">2015<\/h4><\/div><div class=\"uagb-faq-content\"><p>Bl\u00e1ha J., Pachl P., Nov\u00e1k P., Van\u011bk O. (2015): Expression and purification of soluble and stable ectodomain of natural killer cell receptor LLT1 through high-density transfection of suspension adapted HEK293S GnTI<sup>&#8211;<\/sup>\u00a0cells.\u00a0<em>Protein Expr Purif<\/em>\u00a0109, 7-13.\u00a0<strong>IF 1.695<\/strong>\u00a0DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1016\/j.pep.2015.01.006\" target=\"_blank\" rel=\"noreferrer noopener\">10.1016\/j.pep.2015.01.006<\/a><br><br>Sk\u00e1lov\u00e1 T., Bl\u00e1ha J., Harlos K., Du\u0161kov\u00e1 J., Kova\u013e T., Str\u00e1nsk\u00fd J., Ha\u0161ek J., Van\u011bk O., Dohn\u00e1lek J. (2015): Four crystal structures of human LLT1, a ligand for human NKR-P1, in varied glycosylation and oligomerization states.\u00a0<em>Acta Crystallogr Sect D Biol Crystallogr<\/em>\u00a071, 578-591.\u00a0<strong>IF 2.680<\/strong> DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1107\/S1399004714027928\" target=\"_blank\" rel=\"noreferrer noopener\">10.1107\/S1399004714027928<\/a><br><br>Machov\u00e1 I., Sn\u00e1\u0161el J., Dost\u00e1l J., Brynda J., Fanfrl\u00edk J., Singh M., Tar\u00e1bek J., Van\u011bk O., Bedn\u00e1rov\u00e1 L., Pichov\u00e1 I. (2015): Structural and functional studies of phosphoenolpyruvate carboxykinase from Mycobacterium tuberculosis.\u00a0<em>PLoS ONE<\/em>\u00a010, e0120682.\u00a0<strong>IF 3.534<\/strong>\u00a0DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1371\/journal.pone.0120682\" target=\"_blank\" rel=\"noreferrer noopener\">10.1371\/journal.pone.0120682<\/a><br><br>Lynn G. M., Laga R., Darrah P. A., Ishizuka A. S., Balaci A. J., Dulcey A. E., Pechar M., Pola R., Gerner M. Y., Yamamoto A., Buechler C. R., Quinn K. M., Smelkinson M. G., Van\u011bk O., Cawood R., Hills T., Vasalatiy O., Kastenm\u00fcller K., Francica J. R., Stutts L., Tom J. K., Ryu K. A., Esser-Kahn A. P., Etrych T., Fisher K. D., Seymour L. W., Seder R. A. (2015):\u00a0<em>In vivo<\/em>\u00a0characterization of the physicochemical properties of polymer-linked TLR agonists that enhance vaccine immunogenicity.\u00a0<em>Nat Biotechnol<\/em>\u00a033, 1201-1210.\u00a0<strong>IF 43.113<\/strong> \u00a0DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1038\/nbt.3371\" target=\"_blank\" rel=\"noreferrer noopener\">10.1038\/nbt.3371<\/a><\/p><\/div><\/div><\/div>\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-4983","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false},"uagb_author_info":{"display_name":"structimmuno","author_link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/author\/structimmuno\/"},"uagb_comment_info":0,"uagb_excerpt":null,"_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4983","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/comments?post=4983"}],"version-history":[{"count":24,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4983\/revisions"}],"predecessor-version":[{"id":7932,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/4983\/revisions\/7932"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media?parent=4983"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}