{"id":6939,"date":"2023-02-13T11:54:41","date_gmt":"2023-02-13T11:54:41","guid":{"rendered":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/?page_id=6939"},"modified":"2026-06-06T16:06:28","modified_gmt":"2026-06-06T16:06:28","slug":"cv-jan-blaha","status":"publish","type":"page","link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/cv-jan-blaha\/","title":{"rendered":"Curriculum vitae – RNDr. Jan Bl\u00e1ha, Ph.D."},"content":{"rendered":"\n

Postdoctoral fellow<\/em><\/p>\n\n\n\n

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Research profiles<\/h5>\n\n\n\n
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<\/a>\"\"\/<\/span>e-mail: jan.blaha@natur.cuni.cz<\/a><\/span><\/div>\n<\/div><\/div>\n\n\n\n
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Education<\/strong><\/h3>
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2026 \u2013 present<\/div>

Senior Postdoctoral Fellow<\/strong><\/h4>

Department of Biochemistry, Faculty of Science, Charles University
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2026 \u2013 present<\/div><\/article>\n\n\n\n
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2019 \u2013 2025<\/div>

EIPOD4 Postdoctoral fellow, EMBL-Hamburg, Matthias Wilmanns group <\/strong><\/h4>

Integrative structural biology (MX, SSX, SAXS, CEM, uED, AF3), in cellulo<\/em> crystallography, image stream cell sorting, fluorescence microscopy, crystal direct harvester, generation of sybodies, cluster-based data processing (CrystFEL), protein biophysical characterization (MP, BLI, MALLS, DSF), heterologous expression in prokaryotic, insect, and mammalian systems, science public outreach, training, and mentoring students
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2019 \u2013 2025<\/div><\/article>\n\n\n\n
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2017 \u2013 2019<\/div>

Research assistant at the Department of Biochemistry, Faculty of Science, Charles University<\/strong><\/h4>

Research and pedagogical activities with a focus on the study of the structure and interaction of natural killer cell receptors and their usage in translational medicine. Preparation of scientific articles and grant applications.\u00a0<\/em>
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2017 \u2013 2019<\/div><\/article>\n\n\n\n
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2017<\/div>

Ph.D. in Biochemistry<\/strong><\/h4>

Department of Biochemistry, Faculty of Science, Charles University; doctoral thesis \u201cStudy of structure and interaction of human lymphocyte receptors\u201d
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2017<\/div><\/article>\n\n\n\n
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2012 \u2013 2017<\/div>

Researcher in the Laboratory of Structural Biochemistry of Immune Recognition<\/strong>
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LIC cloning techniques, heterologous expression in prokaryotic and eukaryotic systems, protein purification (IMAC, IEX, HIC, SEC), automation, biophysical characterization (AUC, SPR, MST, CD), MX and SAXS diffraction data processing, super-resolution microscopy (dSTORM, PALM), Voronoi tessellation cluster analysis
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2012 \u2013 2017<\/div><\/article>\n\n\n\n
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2012<\/div>

Master’s degree in Biochemistry<\/strong><\/h4>

Department of Biochemistry, Faculty of Science, Charles University; master’s thesis \u201cPreparation and study of human lymphocyte receptor LLT1\u201d
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2012<\/div><\/article>\n\n\n\n
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2010 \u2013 2011<\/div>

Researcher in the Laboratory of Protein Architecture, IMB CAS CZ<\/strong><\/h4>

DNA cloning, suspension\/adherent cultivation of eukaryotic cells, heterologous expression in HEK293, enzyme kinetics, IMAC<\/p>

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2010 \u2013 2011<\/div><\/article>\n\n\n\n
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2010<\/div>

Bachelor’s degree in Biochemistry <\/strong><\/h4>

Department of Biochemistry, Faculty of Science, Charles University; bachelor’s thesis \u201cOptimization of recombinant protein expression in HEK293 cell line\u201d<\/p>

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2010<\/div><\/article>\n
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Language skills<\/strong><\/h3>
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<\/a>\"\"\/<\/span>Czech – mother language<\/span><\/div>\n\n\n\n
<\/a>\"\"\/<\/span>English – CAE, (C2, 2013)<\/span><\/div>\n\n\n\n
<\/a>\"\"\/<\/span>French – DELF (B1, 2006)\"\"<\/span><\/div>\n\n\n\n
<\/a>\"\"\/<\/span>German – Elementary proficiency<\/span><\/div>\n\n\n\n
<\/a>\"\"\/<\/span>Japanese – Elementary proficiency<\/span><\/div>\n<\/div><\/div>\n\n\n\n
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Publication<\/strong>s<\/h3>
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2025<\/td>Nejadebrahim S, Houserov\u00e1 J, Je\u010dmen T, Kalouskov\u00e1 B, Abreu C, Herynek \u0160, Sko\u0159epa O, Bl\u00e1ha J, Van\u011bk O. Multiple O- and an N-glycosylation of the stalk region of the NK cell activation receptor NKp46 mediates its interaction with the Candida glabrata<\/em> epithelial adhesin 1.<\/strong> International Journal of Biological Macromolecules.<\/em> 2025;310:143037. https:\/\/doi.org\/10.1016\/j.ijbiomac.2025.143037<\/a><\/td><\/tr>
2025<\/td>Abreu C, Di Carluccio C, Je\u010dmen T, Sko\u0159epa O, Bl\u00e1ha J, Marchetti R, Silipo A, Van\u011bk O. Insights into stability, dimerisation, and ligand binding properties of Siglec-7: Isotope labelling in HEK293 cells for protein characterisation by NMR spectroscopy.<\/strong> International Journal of Biological Macromolecules.<\/em> 2025;309(Pt 1):142672. https:\/\/doi.org\/10.1016\/j.ijbiomac.2025.142672<\/a><\/td><\/tr>
2022<\/td>Bl\u00e1ha J, Sk\u00e1lov\u00e1 T, Kalouskov\u00e1 B, Sko\u0159epa O, Cmunt D, Grob\u00e1rov\u00e1 V, Pazicky S, Pol\u00e1chov\u00e1 E, Str\u00e1nsk\u00fd J, Kova\u013e T, Du\u0161kov\u00e1 J, Zhao Y, Harlos K, Ha\u0161ek J, Dohn\u00e1lek J, Van\u011bk O. Structure of the human NK cell NKR-P1:LLT1 receptor:ligand complex reveals clustering in the immune synapse.<\/strong> Nature Communications.<\/em> 2022;13(1):5022. https:\/\/doi.org\/10.1038\/s41467-022-32577-6<\/a><\/td><\/tr>
2021<\/td>Kalouskov\u00e1 B, Sko\u0159epa O, Cmunt D, Abreu C, Krej\u010dov\u00e1 K, Bl\u00e1ha J, Sieglov\u00e1 I, Kr\u00e1l V, F\u00e1bry M, Pola R, Pechar M, Van\u011bk O. Tumor Marker B7-H6 Bound to the Coiled Coil Peptide-Polymer Conjugate Enables Targeted Therapy by Activating Human Natural Killer Cells.<\/strong> Biomedicines.<\/em> 2021;9(11):1597. https:\/\/doi.org\/10.3390\/biomedicines9111597<\/a><\/td><\/tr>
2020<\/td>Sko\u0159epa O, Pa\u017eick\u00fd S, Kalouskov\u00e1 B, Bl\u00e1ha J, Abreu C, Je\u010dmen T, Ros\u016flek M, Fish A, Sediv\u00fd A, Harlos K, Dohn\u00e1lek J, Sk\u00e1lov\u00e1 T, Van\u011bk O. Natural Killer Cell Activation Receptor NKp30 Oligomerization Depends on Its N-Glycosylation.<\/strong> Cancers.<\/em> 2020;12(7):1998. https:\/\/doi.org\/10.3390\/cancers12071998<\/a><\/td><\/tr>
2020<\/td>Sk\u00e1lov\u00e1 T, Leng\u00e1lov\u00e1 A, Dohn\u00e1lek J, Harlos K, Mihal\u010din P, Kolenko P, Stranava M, Bl\u00e1ha J, Shimizu T, Mart\u00ednkov\u00e1 M. Disruption of the dimerization interface of the sensing domain in the dimeric heme-based oxygen sensor AfGcHK abolishes bacterial signal transduction.<\/strong> Journal of Biological Chemistry.<\/em> 2020;295(6):1587\u20131597. https:\/\/doi.org\/10.1074\/jbc.RA119.011574<\/a><\/td><\/tr>
2019<\/td>Van\u011bk O, \u010celadov\u00e1 P, Sko\u0159epa O, Bl\u00e1ha J, Kalouskov\u00e1 B, Dvorsk\u00e1 A, Pol\u00e1chov\u00e1 E, Pucholtov\u00e1 H, Kavan D, Pompach P, Hofbauerov\u00e1 K, Kopeck\u00fd V, Mesci A, Voigt S, Carlyle JR. Production of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells.<\/strong> Scientific Reports.<\/em> 2019;9(1):17836. https:\/\/doi.org\/10.1038\/s41598-019-52114-8<\/a><\/td><\/tr>
2018<\/td>\u0160kerlov\u00e1 J, Bl\u00e1ha J, Pachl P, Hofbauerov\u00e1 K, Kuka\u010dka Z, Man P, Pompach P, Nov\u00e1k P, Otwinowski Z, Brynda J, Van\u011bk O, \u0158ez\u00e1\u010dov\u00e1 P. Crystal structure of native \u03b2-N-acetylhexosaminidase isolated from Aspergillus oryzae<\/em> sheds light onto its substrate specificity, high stability, and regulation by propeptide.<\/strong> The FEBS Journal.<\/em> 2018;285(3):580\u2013598. https:\/\/doi.org\/10.1111\/febs.14360<\/a><\/td><\/tr>
2017<\/td>Stranava M, Man P, Sk\u00e1lov\u00e1 T, Kolenko P, Bl\u00e1ha J, Fojt\u00edkov\u00e1 V, Mart\u00ednek V, Dohn\u00e1lek J, Leng\u00e1lov\u00e1 A, Ros\u016flek M, Shimizu T, Mart\u00ednkov\u00e1 M. Coordination and redox state-dependent structural changes of the heme-based oxygen sensor AfGcHK associated with intraprotein signal transduction.<\/strong> Journal of Biological Chemistry.<\/em> 2017;292(51):20921\u201320935. https:\/\/doi.org\/10.1074\/jbc.M117.817023<\/a><\/td><\/tr>
2017<\/td>Bl\u00e1ha J, Kalouskov\u00e1 B, Sko\u0159epa O, Pa\u017eick\u00fd S, Nov\u00e1k P, Van\u011bk O. High-level expression and purification of soluble form of human natural killer cell receptor NKR-P1 in HEK293S GnTI\u2212 cells.<\/strong> Protein Expression and Purification.<\/em> 2017;140:36\u201343. https:\/\/doi.org\/10.1016\/j.pep.2017.07.016<\/a><\/td><\/tr>
2015<\/td>Bl\u00e1ha J, Pachl P, Nov\u00e1k P, Van\u011bk O. Expression and purification of soluble and stable ectodomain of natural killer cell receptor LLT1 through high-density transfection of suspension adapted HEK293S GnTI(-) cells.<\/strong> Protein Expression and Purification.<\/em> 2015;109:7\u201313. https:\/\/doi.org\/10.1016\/j.pep.2015.01.006<\/a><\/td><\/tr>
2015<\/td>Sk\u00e1lov\u00e1 T, Bl\u00e1ha J, Harlos K, Du\u0161kov\u00e1 J, Kova\u013e T, Str\u00e1nsk\u00fd J, Ha\u0161ek J, Van\u011bk O, Dohn\u00e1lek J. Four crystal structures of human LLT1, a ligand of human NKR-P1, in varied glycosylation and oligomerization states.<\/strong> Acta Crystallographica Section D: Biological Crystallography.<\/em> 2015;71(Pt 3):578\u2013591. https:\/\/doi.org\/10.1107\/S1399004714027928<\/a>
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Research projects since obtaining the Ph.D.<\/strong><\/h3>
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1.<\/td>Ambient ion soft landing as reproducible sample deposition on grids for cryo-electron microscopy. MERIT fellowship (Marie Sklodowska-Curie cofund fellowship, 2025-2027, rejected), principal investigator<\/strong><\/td><\/tr>
2.<\/td>Efficient in-cellulo X-ray data acquisition using advanced sample enrichment and delivery tools (Life Science Alliance fund, Stanford University, 2022-2024) principal investigator<\/strong><\/td><\/tr>
3.<\/td>Living cells: Crystallization chambers for serial synchrotron crystallography. EIPOD4 fellowship (Marie Sklodowska-Curie 847543), principal investigator<\/strong><\/td><\/tr>
4.<\/td>Expanding the domain of serial crystallography: Membrane protein & IN CELLULO<\/em> crystallozation (InCellSX). Rontgen-Angstrom-Cluster, 2019-2023, member of research team<\/strong><\/td><\/tr>
5.<\/td>New ligands for old receptors of human natural killer cells: structure, assembly within the immune synapse and potential for therapy. Czech Science Foundation (18-10687S), 2018-2020, member of research team<\/strong><\/td><\/tr>
6.<\/td>INSTRUCT R&D Pilot project award, 2017, member of the research team<\/strong><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Selected Special Courses and Workshops<\/strong><\/h3>
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2024<\/td>BioXFEL spring symposium at ASU<\/td><\/tr>
2023<\/td>Train the trainers \u2013 FAIR policies in data management<\/td><\/tr>
2022<\/td>Approaches for in cellulo structural biology with X-rays<\/a> (organizer<\/strong>)<\/td><\/tr>
2021<\/td>Recent advances in structural biology of membrane proteins<\/td><\/tr>
2020<\/td>EMBO Laboratory leadership <\/strong><\/td><\/tr>
2019<\/td>EMBO Practical Course: The Fundamentals of High-End Cell Sorting, EMBL Heidelberg<\/td><\/tr>
2018<\/td>FEBS & Instruct Practical Course Hydrodynamic and thermodynamic analysis of biological macromolecules and their interactions<\/a> (co-organizer<\/strong>)<\/td><\/tr>
2017<\/td>Proteins for Life<\/a> (co-organizer) <\/strong>international conferences<\/td><\/tr>
2015<\/td>Protein crystallography summer school, University of St. Andrews<\/td><\/tr>
2014<\/td>Advanced methods to study complexes and difficult targets, INSTRUCT, IGBMC, Strasbourg<\/td><\/tr>
2013<\/td>Time management training course, Flowenta<\/td><\/tr>
2012<\/td>Advanced methods in macromolecular crystallization V, FEBS, Nov\u00e9 Hrady<\/td><\/tr>
2011<\/td>Mammalian expression workshop, P-CUBE, STRUBI, Oxford<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Internships and visits abroad<\/strong><\/strong><\/h3>
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2017<\/td>SPR platform (INSTRUCT access, 1 week), IBS, Grenoble, France<\/td><\/tr>
2016<\/td>Two-month-long Instruct internship in David Stuart\u2019s group, STRUBI,
University of Oxford, UK; (Dr. Yuguang Zhao as supervisor)<\/td><\/tr>
2012 \u2013 2013<\/td>HTP prokaryotic and baculoviral expression platform (2\u00d7 INSTRUCT access,
2 weeks total), OPPF, Oxford, UK<\/td><\/tr>
2011 \u2013 2015<\/td>HTP mammalian expression and protein crystallization platform
(4\u00d7 INSTRUCT access, 4 weeks total), STRUBI, University of Oxford, UK <\/strong><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div><\/div>\n\n\n\n
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Awards<\/strong><\/strong><\/strong><\/h3>
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2015<\/td>3rd<\/sup> prize<\/strong> for the best oral presentation in the English language, PGS conference at XVII. Meeting of Biochemists and Molecular Biologists<\/td><\/tr>
2012<\/td>2nd<\/sup> prize<\/strong> for the best oral presentation in the English language, Young section of XXIII. Biochemical meeting (2012), Brno, Czech Republic<\/td><\/tr>
2005<\/td>Best Video<\/strong> of Physics Experiment as Part of the World Year of Physics<\/td><\/tr>
2005<\/td>4th<\/sup> prize<\/strong> in SPUSA English writing competition <\/strong><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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TEACHING ACTIVITIES AND PUBLIC OUTREACH<\/strong><\/strong><\/strong><\/strong><\/h3>
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2022 \u2013 2022<\/td>Public Science Outreach at the Natural History Museum in London, UK<\/td><\/tr>
2019 \u2013 2024<\/td>Supervision of short-term visiting students and one master student<\/td><\/tr>
2017 \u2013 2019<\/td>Coordinator for ERASMUS+ at the Department of Biochemistry, Charles University<\/td><\/tr>
2017 \u2013 2017<\/td>3rd Journey into the depths of chemistry for high schools<\/td><\/tr>
2012 \u2013 2019<\/td>Advanced practical course in Biochemistry (MC250C17N), Charles University<\/td><\/tr>
2012 \u2013 2019<\/td>Advanced practical course in Biochemistry for KATA (MC250C03), Charles University<\/td><\/tr>
2007 \u2013 2010 <\/td>Lecturer of chemistry at the Natural Sciences Station
competition\u00a0<\/strong><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Other abilities<\/strong><\/h3>
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Batch scripting, Python-oriented programming. Woodworking, cross-stitching, knitting, acrylic painting, 3D printing, chess, Rubik\u2019s puzzles. Active runner, passionate cook, avid reader.<\/p><\/div>\n\n\n\n

Last updated 6. 6. 2026<\/em><\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

Postdoctoral fellow Education Language skills Publications 2025 Nejadebrahim S, Houserov\u00e1 J, Je\u010dmen T, Kalouskov\u00e1 B, Abreu C, Herynek \u0160, Sko\u0159epa O, Bl\u00e1ha J, Van\u011bk O. Multiple O- and an N-glycosylation of the stalk region of the NK cell activation receptor NKp46 mediates its interaction with the Candida glabrata epithelial adhesin 1. International Journal of Biological […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-6939","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false},"uagb_author_info":{"display_name":"structimmuno","author_link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/author\/structimmuno\/"},"uagb_comment_info":0,"uagb_excerpt":"Postdoctoral fellow Education Language skills Publications 2025 Nejadebrahim S, Houserov\u00e1 J, Je\u010dmen T, Kalouskov\u00e1 B, Abreu C, Herynek \u0160, Sko\u0159epa O, Bl\u00e1ha J, Van\u011bk O. Multiple O- and an N-glycosylation of the stalk region of the NK cell activation receptor NKp46 mediates its interaction with the Candida glabrata epithelial adhesin 1. International Journal of Biological…","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/6939","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/comments?post=6939"}],"version-history":[{"count":32,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/6939\/revisions"}],"predecessor-version":[{"id":8074,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/pages\/6939\/revisions\/8074"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media?parent=6939"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}