{"id":7647,"date":"2025-04-16T13:08:10","date_gmt":"2025-04-16T13:08:10","guid":{"rendered":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/?p=7647"},"modified":"2025-06-20T17:24:03","modified_gmt":"2025-06-20T17:24:03","slug":"a-new-paper-has-been-published-in-cooperation-with-an-italian-laboratory-of-prof-alba-silipo","status":"publish","type":"post","link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/a-new-paper-has-been-published-in-cooperation-with-an-italian-laboratory-of-prof-alba-silipo\/","title":{"rendered":"A new paper has been published in cooperation with an Italian laboratory of prof. Alba Silipo!"},"content":{"rendered":"\n

<\/p>\n\n\n\n

Our PhD student Celeste Abreu<\/strong> is the first author of a new paper published in the International Journal of Biological Macromolecules<\/em> titled:
“Insights into stability, dimerisation, and ligand binding properties of Siglec-7: Isotope labelling in HEK293 cells for protein characterisation by NMR spectroscopy”<\/strong><\/p>\n\n\n\n

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3D model of disialylated pentaose bound to Siglec-7_CRD (PDB 2HRL)<\/em><\/p>\n\n\n\n

<\/p>\n<\/div><\/div>\n\n\n\n

The study reports that the extracellular domain of Siglec-7<\/strong> forms a noncovalent dimer<\/strong>, which may impact ligand binding and signalling. It also describes a method for producing double isotopically labelled Siglec-7 in HEK293 cells, suitable for NMR-based structural studies<\/strong>. This work provides new insights into Siglec-7 biology and a protocol applicable to other glycoproteins.<\/p>\n\n\n\n

<\/p>\n\n\n\n

Abreu, C., Di Carluccio, C., Je\u010dmen, T., Sko\u0159epa, O., Bl\u00e1ha, J., Marchetti, R., Silipo, A., Van\u011bk, O. (2025): Insights into stability, dimerisation, and ligand binding properties of Siglec-7: Isotope labelling in HEK293 cells for protein characterisation by NMR spectroscopy. Int. J. Biol. Macromol. <\/em>309, 142672. IF 8.5<\/strong> DOI: 10.1016\/j.ijbiomac.2025.142672<\/a><\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

Our PhD student Celeste Abreu is the first author of a new paper published in the International Journal of Biological Macromolecules titled:“Insights into stability, dimerisation, and ligand binding properties of Siglec-7: Isotope labelling in HEK293 cells for protein characterisation by NMR spectroscopy” 3D model of disialylated pentaose bound to Siglec-7_CRD (PDB 2HRL) The study reports […]<\/p>\n","protected":false},"author":1,"featured_media":7649,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[16],"tags":[],"class_list":["post-7647","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications"],"uagb_featured_image_src":{"full":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/Celeste-c.jpg",1260,886,false],"thumbnail":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/Celeste-c-150x150.jpg",150,150,true],"medium":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/Celeste-c-300x211.jpg",300,211,true],"medium_large":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/Celeste-c-768x540.jpg",640,450,true],"large":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/Celeste-c-1024x720.jpg",640,450,true],"1536x1536":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/Celeste-c.jpg",1260,886,false],"2048x2048":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/Celeste-c.jpg",1260,886,false]},"uagb_author_info":{"display_name":"structimmuno","author_link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/author\/structimmuno\/"},"uagb_comment_info":0,"uagb_excerpt":"Our PhD student Celeste Abreu is the first author of a new paper published in the International Journal of Biological Macromolecules titled:“Insights into stability, dimerisation, and ligand binding properties of Siglec-7: Isotope labelling in HEK293 cells for protein characterisation by NMR spectroscopy” 3D model of disialylated pentaose bound to Siglec-7_CRD (PDB 2HRL) The study reports…","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts\/7647","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/comments?post=7647"}],"version-history":[{"count":10,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts\/7647\/revisions"}],"predecessor-version":[{"id":7750,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts\/7647\/revisions\/7750"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media\/7649"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media?parent=7647"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/categories?post=7647"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/tags?post=7647"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}