{"id":7686,"date":"2025-04-25T08:37:46","date_gmt":"2025-04-25T08:37:46","guid":{"rendered":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/?p=7686"},"modified":"2025-06-20T17:22:22","modified_gmt":"2025-06-20T17:22:22","slug":"new-paper-is-out-find-out-about-nkp46-glycosylation-and-candida-glabrata-interaction","status":"publish","type":"post","link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/new-paper-is-out-find-out-about-nkp46-glycosylation-and-candida-glabrata-interaction\/","title":{"rendered":"New paper is out! Find about NKp46 glycosylation and Candida glabrata interaction"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">We are proud to share the latest publication from our lab member, <strong>Shiva Nejadebrahim<\/strong>, titled <strong>&#8220;Multiple O- and an N-glycosylation of the stalk region of the NK cell activation receptor NKp46 mediates its interaction with the <em>Candida glabrata<\/em> epithelial adhesin 1&#8243;<\/strong>.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">This study provides, for the first time, a comprehensive glycosylation profile of the NK cell activation receptor NKp46, with a focus on its stalk region. By employing mass spectrometry techniques and recombinant protein production, the research uncovers how both <strong>O- and N-glycosylation<\/strong> events contribute to the receptor&#8217;s ability to recognise and bind <strong>Epa1<\/strong>, a key epithelial adhesin from the fungal pathogen <em>Candida glabrata<\/em>.<\/p>\n\n\n\n<div class=\"wp-block-media-text is-stacked-on-mobile\" style=\"grid-template-columns:61% auto\"><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"690\" src=\"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/News-Shiva-1024x690.jpg\" alt=\"\" class=\"wp-image-7687 size-full\" srcset=\"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/News-Shiva-1024x690.jpg 1024w, https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/News-Shiva-300x202.jpg 300w, https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/News-Shiva-768x517.jpg 768w, https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/News-Shiva.jpg 1333w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure><div class=\"wp-block-media-text__content\">\n<p class=\"wp-block-paragraph\"><em>Schematic overview of the interaction between NKp46 on NK cells and Epa1 from Candida glabrata, mediated through complex O- and N-glycosylation patterns on the receptor\u2019s stalk region.<\/em><\/p>\n<\/div><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">The findings demonstrate that multiple glycosylation sites, including novel O-glycosylation patterns and an essential N-glycan, are critical for NKp46\u2019s interaction with Epa1. These insights highlight a previously underappreciated role of glycosylation in modulating innate immune recognition, with implications for fungal pathogenesis and immune evasion.<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"has-vivid-cyan-blue-color has-text-color has-link-color wp-elements-cb66c04a7a919a411c13ce615e75a23e wp-block-paragraph\">Nejadebrahim S., Houserov\u00e1 J., Je\u010dmen T., Kalouskov\u00e1 B., Abreu C., Herynek \u0160., Sko\u0159epa O., Bl\u00e1ha J., Van\u011bk O. (2025): Multiple O- and an N-glycosylation of the stalk region of the NK cell activation receptor NKp46 mediates its interaction with the <em>Candida glabrata<\/em> epithelial adhesin 1. <em>Int. J. Biol. Macromol<\/em>. 310, 143037.\u00a0<strong>IF 8.5<\/strong> DOI:\u00a0<a class=\"\" href=\"https:\/\/doi.org\/10.1016\/j.ijbiomac.2025.143037\">10.1016\/j.ijbiomac.2025.143037<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>We are proud to share the latest publication from our lab member, Shiva Nejadebrahim, titled &#8220;Multiple O- and an N-glycosylation of the stalk region of the NK cell activation receptor NKp46 mediates its interaction with the Candida glabrata epithelial adhesin 1&#8243;. This study provides, for the first time, a comprehensive glycosylation profile of the NK [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":7687,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[16],"tags":[],"class_list":["post-7686","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications"],"uagb_featured_image_src":{"full":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/News-Shiva.jpg",1333,898,false],"thumbnail":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/News-Shiva-150x150.jpg",150,150,true],"medium":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/News-Shiva-300x202.jpg",300,202,true],"medium_large":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/News-Shiva-768x517.jpg",640,431,true],"large":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/News-Shiva-1024x690.jpg",640,431,true],"1536x1536":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/News-Shiva.jpg",1333,898,false],"2048x2048":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/04\/News-Shiva.jpg",1333,898,false]},"uagb_author_info":{"display_name":"structimmuno","author_link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/author\/structimmuno\/"},"uagb_comment_info":0,"uagb_excerpt":"We are proud to share the latest publication from our lab member, Shiva Nejadebrahim, titled &#8220;Multiple O- and an N-glycosylation of the stalk region of the NK cell activation receptor NKp46 mediates its interaction with the Candida glabrata epithelial adhesin 1&#8243;. This study provides, for the first time, a comprehensive glycosylation profile of the NK&hellip;","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts\/7686","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/comments?post=7686"}],"version-history":[{"count":9,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts\/7686\/revisions"}],"predecessor-version":[{"id":7749,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts\/7686\/revisions\/7749"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media\/7687"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media?parent=7686"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/categories?post=7686"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/tags?post=7686"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}