{"id":7849,"date":"2025-11-04T08:01:38","date_gmt":"2025-11-04T08:01:38","guid":{"rendered":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/?p=7849"},"modified":"2025-12-08T23:20:17","modified_gmt":"2025-12-08T23:20:17","slug":"siglec-7-and-immune-evasion-our-research-featured-on-the-faculty-website","status":"publish","type":"post","link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/siglec-7-and-immune-evasion-our-research-featured-on-the-faculty-website\/","title":{"rendered":"Siglec-7 and Immune Evasion \u2013 Our Research Featured on the Faculty Website"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">Our team&#8217;s research has been highlighted in the news section of the Faculty of Science, Charles University! <\/p>\n\n\n\n<p class=\"wp-block-paragraph\">In the article entitled <strong>&#8220;<a href=\"https:\/\/natur.cuni.cz\/en\/news\/9189-how-unwanted-visitors-tricked-the-immune-system\">How Unwanted Visitors Tricked the Immune System<\/a>&#8220;<\/strong>, the study describes how tumour cells and pathogens evade immune surveillance by mimicking host surface glycan structures.<\/p>\n\n\n\n<div class=\"wp-block-media-text is-stacked-on-mobile\" style=\"grid-template-columns:52% auto\"><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"858\" src=\"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-1024x858.jpg\" alt=\"\" class=\"wp-image-7851 size-full\" srcset=\"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-1024x858.jpg 1024w, https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-300x251.jpg 300w, https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-768x644.jpg 768w, https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-1536x1288.jpg 1536w, https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-2048x1717.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure><div class=\"wp-block-media-text__content\">\n<p class=\"wp-block-paragraph\">These invaders exploit sialic acid\u2013decorated \u201csugar ID cards\u201d to engage the inhibitory receptor Siglec-7 on natural killer (NK) cells. When Siglec-7 is engaged, it sends a \u201c<strong>do not attack<\/strong>\u201d signal, allowing the disguised cells to evade immune elimination.<\/p>\n<\/div><\/div>\n\n\n\n<div style=\"height:26px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">In our lab, we characterised the extracellular domain of Siglec-7, demonstrated the presence of a stable dimeric form, and developed a method for producing doubly \u00b9\u00b3C\/\u00b9\u2075N-labelled protein in mammalian cells\u2014enabling detailed NMR structural studies. Thanks to these studies, it was identified that the tumour-associated ganglioside GD3 binds directly to Siglec-7 and that <strong>arginine 124<\/strong> is a key residue mediating this interaction.<\/p>\n\n\n\n<div class=\"wp-block-uagb-separator uagb-block-a3c46bb1\"><div class=\"wp-block-uagb-separator__inner\" style=\"--my-background-image:\"><\/div><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/doi.org\/10.1016\/j.ijbiomac.2025.142672\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Abreu, Celeste,&nbsp;<em>et al<\/em>. \u201cInsights into Stability, Dimerisation, and Ligand Binding Properties of&nbsp;Siglec-7: Isotope Labelling in&nbsp;HEK293 Cells for Protein Characterisation by&nbsp;NMR Spectroscopy.\u201d&nbsp;<em>International Journal of&nbsp;Biological Macromolecules<\/em>&nbsp;309, 142672 (2025). https:\/\/doi.org\/10.1016\/j.ijbiomac.2025.142672<\/mark><\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/doi.org\/10.1002\/advs.202415782\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Di Carluccio, Cristina,&nbsp;<em>et al<\/em>. \u201cInsights into Siglec\u20107 Binding to&nbsp;Gangliosides: NMR Protein Assignment and the Impact of&nbsp;Ligand Flexibility.\u201d&nbsp;<em>Advanced Science<\/em>&nbsp;12, e2415782 (2025). https:\/\/doi.org\/10.1002\/advs.202415782<\/mark><\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/doi.org\/10.1021\/jacsau.5c00214\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Di Carluccio, Cristina,&nbsp;<em>et al<\/em>. \u201cMolecular Basis of&nbsp;Siglec-7 Recognition by&nbsp;<em>Neisseria meningitidis<\/em>&nbsp;Serogroup Y&nbsp;CPS: Implications for Immune Evasion.\u201d&nbsp;<em>JACS Au<\/em>&nbsp;5, 2257\u20132269 (2025). https:\/\/doi.org\/10.1021\/jacsau.5c00214<\/mark><\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/doi.org\/10.1021\/jacsau.5c00810\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Di Carluccio, Cristina,\u00a0et al. \u201cFusobacterium nucleatum\u00a0Lipopolysaccharides O-Antigen Defines a\u00a0Novel Siglec-7 Binding Epitope.\u201d\u00a0<em>JACS Au<\/em> 5, 5367-5380. https:\/\/doi.org\/10.1021\/jacsau.5c00810<\/mark><\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Our team&#8217;s research has been highlighted in the news section of the Faculty of Science, Charles University! In the article entitled &#8220;How Unwanted Visitors Tricked the Immune System&#8220;, the study describes how tumour cells and pathogens evade immune surveillance by mimicking host surface glycan structures. These invaders exploit sialic acid\u2013decorated \u201csugar ID cards\u201d to engage [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":7851,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[16],"tags":[],"class_list":["post-7849","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications"],"uagb_featured_image_src":{"full":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-scaled.jpg",2560,2146,false],"thumbnail":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-150x150.jpg",150,150,true],"medium":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-300x251.jpg",300,251,true],"medium_large":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-768x644.jpg",640,537,true],"large":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-1024x858.jpg",640,536,true],"1536x1536":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-1536x1288.jpg",1536,1288,true],"2048x2048":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/unnamed_2-2048x1717.jpg",2048,1717,true]},"uagb_author_info":{"display_name":"structimmuno","author_link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/author\/structimmuno\/"},"uagb_comment_info":0,"uagb_excerpt":"Our team&#8217;s research has been highlighted in the news section of the Faculty of Science, Charles University! In the article entitled &#8220;How Unwanted Visitors Tricked the Immune System&#8220;, the study describes how tumour cells and pathogens evade immune surveillance by mimicking host surface glycan structures. These invaders exploit sialic acid\u2013decorated \u201csugar ID cards\u201d to engage&hellip;","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts\/7849","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/comments?post=7849"}],"version-history":[{"count":11,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts\/7849\/revisions"}],"predecessor-version":[{"id":7925,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts\/7849\/revisions\/7925"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media\/7851"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media?parent=7849"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/categories?post=7849"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/tags?post=7849"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}