{"id":7859,"date":"2025-11-04T09:49:23","date_gmt":"2025-11-04T09:49:23","guid":{"rendered":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/?p=7859"},"modified":"2025-11-25T05:19:40","modified_gmt":"2025-11-25T05:19:40","slug":"our-work-featured-on-the-cover-of-jacs-au","status":"publish","type":"post","link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/our-work-featured-on-the-cover-of-jacs-au\/","title":{"rendered":"Our Work Featured on the Cover of JACS Au"},"content":{"rendered":"\n<div class=\"wp-block-media-text is-stacked-on-mobile\" style=\"grid-template-columns:42% auto\"><figure class=\"wp-block-media-text__media\"><img decoding=\"async\" src=\"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/jaaucr.2025.5.issue-10.xlargecover-3-770x1024.jpg\" alt=\"\" class=\"wp-image-7861 size-full\"\/><\/figure><div class=\"wp-block-media-text__content\">\n<p class=\"wp-block-paragraph\">We are pleased to share that a new collaborative publication \u201c<strong><a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/jacsau.5c00754\" target=\"_blank\" rel=\"noreferrer noopener\">Engineering a Functional Histidine Brace Copper\u2011Binding Site into a De\u202fNovo\u2011Designed Protein Scaffold<\/a><\/strong>\u201d has been selected for the <strong><a href=\"https:\/\/pubs.acs.org\/toc\/jaaucr\/5\/10\">cover of JACS Au<\/a><\/strong> (Journal of the American Chemical Society \u2013 Au). The cover illustration highlights the main concept of our study and represents a significant recognition from the journal.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">This success has also been shared on <a href=\"https:\/\/www.linkedin.com\/posts\/angela-lombardi-30194916_myacscover-activity-7389440886834757632-dXky\/\">LinkedIn <\/a>by Angela Lombardi, Professor of Inorganic and Bioinorganic Chemistry at the University of Naples Federico II, and the corresponding author of the study.<\/p>\n<\/div><\/div>\n\n\n\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-uagb-separator uagb-block-a04e1b12 is-style-default\"><div class=\"wp-block-uagb-separator__inner\" style=\"--my-background-image:\"><\/div><\/div>\n\n\n\n<h4 class=\"wp-block-heading\">About the study<\/h4>\n\n\n\n<p class=\"wp-block-paragraph\">In this work, we investigated <strong>the design of a de\u202fnovo protein scaffold (named \u201cminiLPMO\u201d)<\/strong> incorporating a histidine\u2011brace copper\u2011binding site. <\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"500\" height=\"266\" src=\"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/au5c00754_0008.webp\" alt=\"\" class=\"wp-image-7863\" style=\"width:469px;height:auto\" srcset=\"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/au5c00754_0008.webp 500w, https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/au5c00754_0008-300x160.webp 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/figure>\n<\/div>\n\n\n<div style=\"height:26px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">Our results show <strong>that the designed protein replicates the spectroscopic features of natural histidine\u2011brace sites and catalyzes cleavage of glycosidic bonds upon hydrogen peroxide activation<\/strong>. This was supported by <strong>circular dichroism, analytical ultracentrifugation, UV\u2011Vis spectroscopy, EPR, pulsed EPR studies, and catalytic assays<\/strong>.<\/p>\n\n\n\n<div class=\"wp-block-uagb-separator uagb-block-bf470e97 is-style-default\"><div class=\"wp-block-uagb-separator__inner\" style=\"--my-background-image:\"><\/div><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">This work is the result of collaboration between the Department of Chemical Sciences, University of Naples\u202fFederico\u202fII, Italy; the Department of Chemistry, University of Torino, Italy; and the Department of Biochemistry, Faculty of Science, Charles University, Czechia. We thank all co-authors and the funding agencies. We&#8217;re also grateful to <a href=\"https:\/\/www.linkedin.com\/in\/alessandra-esposito3\/\">Alessandra Esposito<\/a> for creating this beautiful cover art!<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/doi.org\/10.1021\/jacsau.5c00754\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">La Gatta S., Leone L., Sgueglia G., \u0160imuni\u0107 L., Liao Y., Van\u011bk O., Chino M., Nastri F., Chiesa M., Lombardi A. (2025): Engineering a functional histidine brace copper-binding site into a\u00a0<em>de novo<\/em>-designed protein scaffold. JACS Au 5, 4799-4810.\u00a0<strong>IF 8.7<\/strong>\u00a0DOI:\u00a010.1021\/jacsau.5c00754<\/mark><\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>We are pleased to share that a new collaborative publication \u201cEngineering a Functional Histidine Brace Copper\u2011Binding Site into a De\u202fNovo\u2011Designed Protein Scaffold\u201d has been selected for the cover of JACS Au (Journal of the American Chemical Society \u2013 Au). The cover illustration highlights the main concept of our study and represents a significant recognition from [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":7861,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[16],"tags":[],"class_list":["post-7859","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications"],"uagb_featured_image_src":{"full":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/jaaucr.2025.5.issue-10.xlargecover-3-scaled-e1762245651906.jpg",1924,1357,false],"thumbnail":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/jaaucr.2025.5.issue-10.xlargecover-3-scaled-e1762245651906-150x150.jpg",150,150,true],"medium":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/jaaucr.2025.5.issue-10.xlargecover-3-scaled-e1762245651906-300x212.jpg",300,212,true],"medium_large":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/jaaucr.2025.5.issue-10.xlargecover-3-scaled-e1762245651906-768x542.jpg",640,452,true],"large":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/jaaucr.2025.5.issue-10.xlargecover-3-scaled-e1762245651906-1024x722.jpg",640,451,true],"1536x1536":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/jaaucr.2025.5.issue-10.xlargecover-3-scaled-e1762245651906-1536x1083.jpg",1536,1083,true],"2048x2048":["https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-content\/uploads\/2025\/11\/jaaucr.2025.5.issue-10.xlargecover-3-1540x2048.jpg",1540,2048,true]},"uagb_author_info":{"display_name":"structimmuno","author_link":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/author\/structimmuno\/"},"uagb_comment_info":0,"uagb_excerpt":"We are pleased to share that a new collaborative publication \u201cEngineering a Functional Histidine Brace Copper\u2011Binding Site into a De\u202fNovo\u2011Designed Protein Scaffold\u201d has been selected for the cover of JACS Au (Journal of the American Chemical Society \u2013 Au). The cover illustration highlights the main concept of our study and represents a significant recognition from&hellip;","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts\/7859","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/comments?post=7859"}],"version-history":[{"count":15,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts\/7859\/revisions"}],"predecessor-version":[{"id":7904,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/posts\/7859\/revisions\/7904"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media\/7861"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/media?parent=7859"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/categories?post=7859"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/biochem\/structimmuno\/wp-json\/wp\/v2\/tags?post=7859"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}