{"id":6193,"date":"2024-12-13T15:44:18","date_gmt":"2024-12-13T15:44:18","guid":{"rendered":"http:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/?p=6193"},"modified":"2025-02-26T08:50:28","modified_gmt":"2025-02-26T08:50:28","slug":"single-cell-rna-sequencing-seminar","status":"publish","type":"post","link":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/single-cell-rna-sequencing-seminar\/","title":{"rendered":"Single cell RNA-sequencing seminar"},"content":{"rendered":"\n<p>Several of our lab members participated in a recent seminar held on our faculty termed &#8220;Introduction to the 10x Chromium Platform and Hands-On Experience&#8221;. The seminar opened with presentations from representatives of Genetica and 10x Genomics, who covered the methodologies available on the Chromium X platform and the latest advancements, including updates on the newly released chemistry for single-cell, multiome, and other related applications. This part was followed by a session consisting of short talks focused on hands-on experience. <\/p>\n\n\n\n<p>Zuzana Konvi\u010dkov\u00e1 and Pau Balart-Garc\u00eda, both heading from the Zuzana Musilov\u00e1 Laboratory, gave interesting presentations regarding their experience with acquisition and processing of scRNAseq data from fish eyes and the challenges of working with rare specimens. Janek Ma\u0161ek, the head of the Laboratory of Intercellular Communication, shared his insight and experience regarding the deconvolution of bulk RNA-seq data with scRNA-seq data to gain the most out of both. Finally from our lab, Honza V. gave a presentation about the advantages and disadvantages of integration of multiple scRNAseq datasets. Hel\u010da shared her experience with the isolation of rare cell populations and their enrichment for the purposes of scRNA-seq. <\/p>\n\n\n\n<p>Overall, it was a great opportunity for discussion and sharing knowledge regarding different approaches from sample acquisition to data processing when it comes to scRNA-sequencing. <\/p>\n\n\n\n<div style=\"height:17px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"http:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/24-scrnaseq-seminar-1024x768.jpeg\" alt=\"\" class=\"wp-image-6196\" style=\"width:646px;height:auto\" srcset=\"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/24-scrnaseq-seminar-1024x768.jpeg 1024w, https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/24-scrnaseq-seminar-300x225.jpeg 300w, https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/24-scrnaseq-seminar-768x576.jpeg 768w, https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/24-scrnaseq-seminar-1536x1152.jpeg 1536w, https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/24-scrnaseq-seminar-2048x1536.jpeg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n\n\n<div style=\"height:17px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Several of our lab members participated in a recent seminar held on our faculty termed &#8220;Introduction to the 10x Chromium Platform and Hands-On Experience&#8221;. The seminar opened with presentations from representatives of Genetica and 10x Genomics, who covered the methodologies available on the Chromium X platform and the latest advancements, including updates on the newly [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":6198,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[14],"tags":[],"class_list":["post-6193","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"uagb_featured_image_src":{"full":["https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/scRNAseq-seminar-header.png",1423,798,false],"thumbnail":["https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/scRNAseq-seminar-header-150x150.png",150,150,true],"medium":["https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/scRNAseq-seminar-header-300x168.png",300,168,true],"medium_large":["https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/scRNAseq-seminar-header-768x431.png",640,359,true],"large":["https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/scRNAseq-seminar-header-1024x574.png",640,359,true],"1536x1536":["https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/scRNAseq-seminar-header.png",1423,798,false],"2048x2048":["https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-content\/uploads\/2024\/12\/scRNAseq-seminar-header.png",1423,798,false]},"uagb_author_info":{"display_name":"admin","author_link":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/author\/admin\/"},"uagb_comment_info":0,"uagb_excerpt":"Several of our lab members participated in a recent seminar held on our faculty termed &#8220;Introduction to the 10x Chromium Platform and Hands-On Experience&#8221;. The seminar opened with presentations from representatives of Genetica and 10x Genomics, who covered the methodologies available on the Chromium X platform and the latest advancements, including updates on the newly&hellip;","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-json\/wp\/v2\/posts\/6193","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-json\/wp\/v2\/comments?post=6193"}],"version-history":[{"count":5,"href":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-json\/wp\/v2\/posts\/6193\/revisions"}],"predecessor-version":[{"id":6200,"href":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-json\/wp\/v2\/posts\/6193\/revisions\/6200"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-json\/wp\/v2\/media\/6198"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-json\/wp\/v2\/media?parent=6193"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-json\/wp\/v2\/categories?post=6193"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/cellbiol\/dobeslab\/wp-json\/wp\/v2\/tags?post=6193"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}