The Zeiss Elyra PS.1 microscope utilizes technologies of structured illumination (SIM) and precise localization (PALM/STORM) to overcome the so-called diffraction barrier, which limits conventional light microscopy to a resolution of around 200 nm. Nanoscopy allows us to observe structures with 2-3x higher resolution with SIM and up to 5 nm with localization microscopy. The microscope utilizes TIRF (Total Internal Reflection Fluorescence) technology to enhance the signal-to-noise ratio in the space immediately adjacent to the cover glass. A set of high-quality lasers and cameras enables us to create exceptional super-resolution images.
Manufacturer: Carl Zeiss Microscopy
Model: Zeiss Elyra PS.1 (inverted)
Location: room S47
Phone: +420 221 951 060
Technical Specifications
Imaging Technology
- Microscope Type: Super-resolution multimodal microscope (SIM, PALM, dSTORM)
- Microscope Stand: Zeiss Axio Observer.Z1
- Lasers Available:
- 405 nm HR Diode Laser (50 mW)
- 488 nm HR Diode Laser (300 mW)
- 561 nm HR DPSS Laser (200 mW)
- 640 nm HR Diode Laser (150 mW)
- Detectors:
- EM-CCD Andor iXon DU 897 camera
- Active pixels 512 x 512; Pixel size 16 μm x 16 μm; Frame rates up to 56 fps (full frame); QE 95%
- sCMOS PCO Edge 5.5 camera
- Active pixels 1280 x 1280; Pixel size 6.5 μm x 6.5 μm; Camera Link; Frame rates up to 100 fps (full frame); QE >60%
- EM-CCD Andor iXon DU 897 camera
- Objectives Available:
- 10x/0.45 Plan-Apochromat (air) WD 2 mm
- 20x/0.8 Plan-Apochromat (air) WD 0.55 mm
- 63x/1.2 C-Apochromat (water) WD 0.28 mm Corr (CG=0.14-0.19 mm)
- 63x/1.46 alpha PlanApo (oil) WD 0.10 mm DIC, Corr (CG=0.15-0.19mm)
- 100x/1.46 alpha PlanApo (oil) WD 0.11 mm DIC
- 100x/1.57* alpha PlanApo (oil) WD 0.11 mm DIC, Corr (CG=0.165-0.175mm)
- Immersion oil with a high refractive index (1.66) and HI cover glasses 0.17mm +/-0.003 are recommended for imaging
- Autofocus:
- HW – Definite Focus (DF) for long-term stability
- Filter Turrets
- Filter set “2” (turret with 6 positions)
- MBS 405/561 + EF BP 420-480/BP 570-630/LP 740
- Laser excitation: 405, 561, 640
- Position: Fixed
- MBS 405/488/642 + EF BP 420-480/BP 495-560/LP 650
- Laser excitation: 405, 488, 640
- Position: Fixed
- MBS 405/488/561/642 + LBF -561/642
- Laser excitation: 561, 640
- Position: Fixed
- MBS 405/488/561 + LBF -488/561
- Laser excitation: 488, 561
- Position: Fixed
- Free position
- Free position
- MBS 405/561 + EF BP 420-480/BP 570-630/LP 740
- Filter set “1” (turret with 6 positions)
- MBS 405 + EF BP 420-480 / LP 750
- Tailored to laser line: 405
- Position: Fixed
- MBS 488 + EF BP 495-550 / LP 750
- Tailored to laser line: 488
- Position: Fixed
- MBS 561 + EF BP 570-620 / LP 750
- Tailored to laser line: 561
- Position: Fixed
- MBS 642 + EF LP 655
- Tailored to laser line: 642
- Position: Fixed
- Tripleband Set DAPI/FITC/TRITC FSet77 HE
- Light source: Epifluorescence lamp
- Position: Exchangeable
- MBS 405 + EF BP 420-480 / LP 750
- Filter set “2” (turret with 6 positions)
Advanced Imaging Capabilities
- Structured Illumination Microscopy (SIM) – resolution ~120 nm (XY)
- Single Molecule Localization (PALM/dSTORM) – resolution ~20-50 nm
- Motorized TIRF (Total Internal Reflection Fluorescence)
- HILO (Highly Inclined and Laminated Optical sheet) imaging
- 3D localization using astigmatism (PRISM)
- Multichannel super-resolution imaging
- Z-stacks and 3D reconstruction of super-resolved data
Live-Cell Imaging Capabilities
- Zeiss Incubation System:
- CO2 Module (CO2 concentration stable between 1 and 8% +/- 0.1%, adjustable) with humidity module, Heating Insert P, and Heating Unit XL
User Information
Access Requirements
- Mandatory user registration
- Specialized super-resolution training is required before independent use
- Users must consult the sample preparation (fluorophore photoswitching) with facility staff
Booking and Usage Policies
- Reservation via the online booking system (Calpendo)
Price
- Pricing for individual services is determined by the current price list. For super-resolution projects, please contact the facility for a specific quote.