The Zeiss Axio Scan.Z1 is a fully automated high-throughput slide scanner designed for rapid, high-quality, and reliable digitization of large numbers of histological, cytological slides, and other samples. The system is ideal for high-volume data projects, high-content screening, and routine image digitization for sharing and analysis.
Manufacturer: Carl Zeiss
Model: Axio Scan.Z1 (Automated Upright Slide Scanner)
Location: room p108 (floor)
Phone: +420 22195 1033
Technical Specifications
Imaging Technology
- Microscope Type: Automated Widefield Scanner
- Imaging Methods: Brightfield and Multi-channel Fluorescence.
- Capacity: Automated scanning of up to 100 standard slides (26 mm x 76 mm) or larger slides (52 mm x 76 mm) with various holder types.
Light Sources and Filters
- Brightfield (Transmitted Light): LED.
- Ring Aperture Contrast – RAC (Transmitted light)
- Fluorescence (Excitation): Fast and gentle Colibri 7 LED unit with high output and rapid wavelength switching among 385 nm, 430 nm, 475 nm, 555 nm, 590 nm, and 630 nm LED sources.
- Alternative Fluorescence Excitation: 735 nm (Colibri must be replaced).
- Filter turret 1: Default configuration for multi-channel acquisition
- 62 HE BFP/GFP/HcRed
- BP 350-390 BS 395 BP 402-448
- BP 460-488 BS 495 BP 500-557
- BP 567-602 BS 610 LP 615
- 70 HE Alexa Fluor 430
- BP 400-460 BS 500 BP 500-600
- 38 HE Green Fluorescent Protein
- BP 450-490 BS 495 BP 500-550
- 64 HE mPlum
- BP 574-599 BS 605 BP 612-682
- 90 HE DAPI/GFP/Cy3/Cy5
- BP 370-400 BS 405 BP 410-440
- BP 450-488 BS 493 BP 499-529
- BP 540-570 BS 575 BP 579-604
- BP 614-647 BS 653 BP659-759
- 62 HE BFP/GFP/HcRed
- Filter turret 2: SEDAT for fast multi-channel acquisition
- Dichroic mirrors
- DM
- Emission filters
- filter
- Dichroic mirrors
Available Objectives (Dry Objectives)
The system utilizes high numerical aperture objectives for maximum resolution and speed without the need for immersion.
- 2.5x/0.12 Fluar
- 5x/0.25 Fluar (WD: 12.5 mm)
- 10x/0.45 Plan-Apochromat (WD: 2.1 mm)
- 20x/0.8 Plan-Apochromat (WD: 0.55 mm)
- 40x/0.95 Plan-Apochromat (WD: 0.25 mm)
- 20x/0.45 N-Achoplan pol
Cameras
- Monochromatic Hamamatsu ORCA Flash 4.0 V3 (C13440-20CU)
- Color camera Hitachi_FD 202 SCL. A high-resolution 3CCD color camera utilizing a prism and a 1.8-inch progressive scan sensor to deliver excellent color fidelity for brightfield virtual microscopy.
Analysis Software
- ZEN lite: a lightweight version of native software for easy viewing and export. Windows only.
- FIJI (FIJI Is Just ImageJ): ImageJ bundled with BioFormats Importer, capable of opening Axioscan data. There is a fundamental constraint in Java that allows opening images only up to approximately 40K x 40K pixels. However, the pyramid structure of files allows for opening a lower resolution or reading cropped regions only.
- QuPath: An open-source software for image processing and analysis.
User Information
Access Requirements
- Mandatory user registration
- Facility-provided training is required before independent use.
Booking and Usage Policies
- Reservation via the online booking system after completing registration and training
- Booking guidelines and facility usage rules were provided during training
Price
- Pricing for individual services is determined by the current price list. Should you have specific questions regarding the pricing of your project, please feel free to contact us.