{"id":5507,"date":"2023-01-11T15:20:45","date_gmt":"2023-01-11T15:20:45","guid":{"rendered":"http:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/?page_id=5507"},"modified":"2026-05-07T11:45:16","modified_gmt":"2026-05-07T09:45:16","slug":"konfmi","status":"publish","type":"page","link":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/konfmi\/","title":{"rendered":"Light Microscopy"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">Services<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">The VMCF Light Microscopy Unit (KONFMI) provides advanced microscopy equipment, expert guidance, and training for users aiming to perform high-quality imaging and analysis of biological samples. Our facility is dedicated to supporting research through state-of-the-art technology and personalized assistance. Our services are open to everybody.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Access to advanced light microscopy systems (confocal, widefield, lightsheet, fluorescence microscopy, super-resolution microscopy, stereo-microscopy, high-end environmental chamber, injector)<\/li>\n\n\n\n<li>Access to advanced microscopy modalities (High Throughput Imaging, FRAP, FRET, FLIM, FCS &amp; FCCS, TIRF, PALM, STORM, SIM, IRM, Two-Photon, SHG)<\/li>\n\n\n\n<li>Personalized user training and technical assistance<\/li>\n\n\n\n<li>Sample preparation and experiment design consultations<\/li>\n\n\n\n<li>Image processing and data analysis support<\/li>\n\n\n\n<li>Workshops and courses on microscopy techniques<\/li>\n\n\n\n<li>Preparation of paraffin-embedded samples<\/li>\n\n\n\n<li>3D printing<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Equipment<\/h2>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h3 class=\"wp-block-heading\">Wide-Field Fluorescence Microscopes:<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/konfmi\/nikon-tie2\/\" data-type=\"page\" data-id=\"6449\">Nikon TiE2<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/konfmi\/zeiss-axioscan-z1\/\" data-type=\"page\" data-id=\"6739\">Zeiss Axioscan Z1<\/a><\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h3 class=\"wp-block-heading\">Stereo Microscopes:<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Zeiss Axiozoom V1 with apotome<\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h3 class=\"wp-block-heading\">Confocal Microscopes:<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/konfmi\/leica-tcs-sp8\/\" data-type=\"page\" data-id=\"6413\">Leica TCS SP8<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/konfmi\/zeiss-lsm-880-nlo\/\" data-type=\"page\" data-id=\"6424\">Zeiss LSM880 NLO<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/konfmi\/raman\/\" data-type=\"page\" data-id=\"6947\">WITec Alpha300 Ri (Raman)<\/a><\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h3 class=\"wp-block-heading\">Light Sheet Microscopes:<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Zeiss Lightsheet Z.1<\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h3 class=\"wp-block-heading\">Super-Resolution Microscopes:<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/konfmi\/elyra-ps1\/\" data-type=\"page\" data-id=\"6994\">Zeiss Elyra PS.1<\/a><\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h3 class=\"wp-block-heading\">3D printers:<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Prusa MKII<\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h3 class=\"wp-block-heading\">Environmental Chambers:<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>OKO-LAB High-end environmental chamber with cooling and O<sub>2<\/sub> level control<\/li>\n\n\n\n<li>OKO-LAB<\/li>\n\n\n\n<li>Zeiss<\/li>\n\n\n\n<li>Pecon<\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading\">Price<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">The <a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/konfmi\/price-list\/\" data-type=\"page\" data-id=\"6484\">current price list <\/a>determines pricing for individual services. If you have specific questions regarding the pricing of your project, please don&#8217;t hesitate to contact us.<\/p>\n<\/div><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Reservations<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">To access equipment at the KONFMI facility, users must first complete a registration process and obtain training from our staff. Independent use of microscopes requires users to be familiar with and able to correctly set up <a href=\"https:\/\/www.microscopyu.com\/tutorials\/kohler\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">K\u00f6hler illumination<\/a>. Once registered and trained, users can reserve microscopes and other equipment via the <a href=\"http:\/\/calpendo.natur.cuni.cz\" data-type=\"link\" data-id=\"calpendo.natur.cuni.cz\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">online booking system<\/a>. For assistance with registration, training, or reservations, please contact the <a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/contacts\/#KONFMI-admins\" data-type=\"page\" data-id=\"5002\">facility administrators<\/a>.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">User&#8217;s workflow<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/general-terms-of-use\/\" data-type=\"page\" data-id=\"5776\">Read the VMCF&#8217;s Terms of Use<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/contacts\/#KONFMI-admins\">Let us know your interest<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/vmcf-user-registration-guide\/\" data-type=\"page\" data-id=\"6283\">Register as a facility user<\/a><\/li>\n\n\n\n<li>Sign in to the <a href=\"http:\/\/calpendo.natur.cuni.cz\" data-type=\"link\" data-id=\"calpendo.natur.cuni.cz\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">booking system<\/a><\/li>\n\n\n\n<li>Enroll in &#8220;<a href=\"https:\/\/calpendo.natur.cuni.cz\/program.html#availableTrainings\">General Welcome Meeting for new users<\/a>&#8220;<\/li>\n\n\n\n<li>Enroll in specific equipment <a href=\"https:\/\/calpendo.natur.cuni.cz\/program.html#availableTrainings\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">training<\/a><\/li>\n\n\n\n<li>Do your research<\/li>\n\n\n\n<li><a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/publications\/how-to-cite-vmcf\/\" data-type=\"page\" data-id=\"5850\">Cite VMCF in your publication<\/a><\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Services The VMCF Light Microscopy Unit (KONFMI) provides advanced microscopy equipment, expert guidance, and training for users aiming to perform high-quality imaging and analysis of biological samples. Our facility is dedicated to supporting research through state-of-the-art technology and personalized assistance. Our services are open to everybody. Equipment Wide-Field Fluorescence Microscopes: Stereo Microscopes: Confocal Microscopes: Light [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-5507","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"wpsso-schema-1x1":false,"wpsso-schema-4x3":false,"wpsso-schema-16x9":false,"wpsso-thumbnail":false,"wpsso-opengraph":false,"wpsso-tc-summary":false,"wpsso-tc-lrgimg":false},"uagb_author_info":{"display_name":"admin","author_link":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/author\/admin\/"},"uagb_comment_info":0,"uagb_excerpt":"Services The VMCF Light Microscopy Unit (KONFMI) provides advanced microscopy equipment, expert guidance, and training for users aiming to perform high-quality imaging and analysis of biological samples. Our facility is dedicated to supporting research through state-of-the-art technology and personalized assistance. Our services are open to everybody. Equipment Wide-Field Fluorescence Microscopes: Stereo Microscopes: Confocal Microscopes: Light&hellip;","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/5507","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/comments?post=5507"}],"version-history":[{"count":12,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/5507\/revisions"}],"predecessor-version":[{"id":7066,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/5507\/revisions\/7066"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/media?parent=5507"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}