{"id":6413,"date":"2025-03-21T17:48:07","date_gmt":"2025-03-21T15:48:07","guid":{"rendered":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/?page_id=6413"},"modified":"2026-02-25T10:13:03","modified_gmt":"2026-02-25T08:13:03","slug":"leica-tcs-sp8","status":"publish","type":"page","link":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/konfmi\/leica-tcs-sp8\/","title":{"rendered":"Leica TCS SP8"},"content":{"rendered":"\n<div class=\"wp-block-media-text is-stacked-on-mobile\"><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"898\" height=\"897\" src=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg\" alt=\"\" class=\"wp-image-6414 size-full\" srcset=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg 898w, https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8-300x300.jpg 300w, https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8-150x150.jpg 150w, https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8-768x767.jpg 768w, https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8-12x12.jpg 12w\" sizes=\"auto, (max-width: 898px) 100vw, 898px\" \/><\/figure><div class=\"wp-block-media-text__content\">\n<p class=\"wp-block-paragraph\">The Leica TCS SP8 is a high-end inverted confocal microscope designed for advanced fluorescence imaging of fixed and live biological samples. It offers outstanding sensitivity, resolution, and versatility suitable for various applications, including cell biology, developmental biology, neurobiology, plant sciences, and more.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Manufacturer:<\/strong> <a href=\"https:\/\/www.leica-microsystems.com\/\" data-type=\"link\" data-id=\"https:\/\/www.leica-microsystems.com\/\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">Leica Microsystems<\/a><br><strong>Model:<\/strong> Leica TCS SP8 (inverted)<br><strong>Location:<\/strong> room S39<br><strong>Phone: <\/strong>+420 22195 1943<\/p>\n<\/div><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Technical Specifications<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Imaging Technology<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Microscope Type:<\/strong> Inverted laser scanning confocal microscope<\/li>\n\n\n\n<li><strong>Microscope Stand: <\/strong>Leica DMi-8<\/li>\n\n\n\n<li><strong>Lasers Available:<\/strong>\n<ul class=\"wp-block-list\">\n<li>Multiple excitation lasers (405 nm, Argon laser 458, 488, 514 nm, 561 nm, 633 nm)<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Detectors:<\/strong>\n<ul class=\"wp-block-list\">\n<li>One sensitive Hybrid (HyD) detector for high-quality imaging<\/li>\n\n\n\n<li>One standard Photomultiplier Tube (PMT) detector<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Objectives Available:<\/strong>\n<ul class=\"wp-block-list\">\n<li>10x\/0.3 WD 11000 \u03bcm (air)<\/li>\n\n\n\n<li>20x\/0.75 WD 660 \u03bcm (multi-immersion options: Oil, Glycerol, Water)<\/li>\n\n\n\n<li>63x\/1.2 WD 140 \u03bcm (high NA water immersion)<\/li>\n\n\n\n<li>63x\/1.4 WD 300 \u03bcm (high NA Oil immersion)<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Autofocus:<\/strong>\n<ul class=\"wp-block-list\">\n<li>HW &#8211; Adaptive Focus Control (AFC)<\/li>\n\n\n\n<li>SF &#8211; Best Focus<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Advanced Imaging Capabilities<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Lambda scanning (spectral imaging)<\/li>\n\n\n\n<li>Multi-channel simultaneous imaging<\/li>\n\n\n\n<li>Z-stacks and 3D reconstruction<\/li>\n\n\n\n<li>Time-lapse imaging and real-time observations<\/li>\n\n\n\n<li>Multiposition,  Tile, and Regions scanning<\/li>\n\n\n\n<li>FRAP (Fluorescence Recovery After Photobleaching)<\/li>\n\n\n\n<li>FRET (F\u00f6rster Resonance Energy Transfer)<\/li>\n\n\n\n<li>MATRIX software module for automated object tracking (e.g., growing roots, oocytes)<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Live-Cell Imaging Capabilities<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>PECON environmental chamber:\n<ul class=\"wp-block-list\">\n<li>Temperature control<\/li>\n\n\n\n<li>CO\u2082 concentration control for maintaining physiological conditions<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li>OKO-LAB Bold Line environmental chamber (optionally):\n<ul class=\"wp-block-list\">\n<li>Temperature control (-5\u00b0C &#8211; 60\u00b0C)<\/li>\n\n\n\n<li>O<sub>2<\/sub> (0-20%) \/ CO\u2082 (1-95%) concentration control for maintaining physiological conditions<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">User Information<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Access Requirements<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Mandatory <a href=\"\/sekce-bi\/VMCF\/vmcf-user-registration-guide\/\">user registration<\/a><\/li>\n\n\n\n<li>Facility-provided <a href=\"https:\/\/calpendo.natur.cuni.cz\/program.html#availableTrainings\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">training<\/a> is required before independent use<\/li>\n\n\n\n<li>Users must understand and demonstrate the correct setup of <a href=\"https:\/\/www.microscopyu.com\/tutorials\/kohler\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">K\u00f6hler illumination<\/a> for independent microscope operation<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Booking and Usage Policies<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Reservation via the <a href=\"https:\/\/calpendo.natur.cuni.cz\/\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">online booking system<\/a> after completing registration and training<\/li>\n\n\n\n<li>Booking guidelines and facility usage rules were provided during training<\/li>\n<\/ul>\n\n\n\n<iframe loading=\"lazy\" \n  src=\"https:\/\/calpendo.natur.cuni.cz\/anon\/WeeklyCalendar.html?title=Confocals%20Weekly&#038;pks=20231003.090934.2.326\" \n  width=\"100%\" \n  height=\"600\" \n  style=\"border:0;\"\n  title=\"Confocals Weekly Calendar\"\n  allowfullscreen>\n<\/iframe>\n\n\n\n<h3 class=\"wp-block-heading\">Price<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Pricing for individual services is determined by the current <a href=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/konfmi\/price-list\/\" data-type=\"page\" data-id=\"6484\">price list<\/a>. Should you have specific questions regarding the pricing of your project, please feel free to contact us.<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<div class=\"wp-block-uagb-container uagb-block-3656525b alignfull uagb-is-root-container\"><div class=\"uagb-container-inner-blocks-wrap\">\n<h2 class=\"wp-block-heading\">Troubleshooting<\/h2>\n\n\n\n<div class=\"wp-block-uagb-faq uagb-faq__outer-wrap uagb-block-3cb66ab7 uagb-faq-icon-row uagb-faq-layout-accordion uagb-faq-expand-first-false uagb-faq-inactive-other-true uagb-faq__wrap uagb-buttons-layout-wrap uagb-faq-equal-height\" data-faqtoggle=\"false\" role=\"tablist\">\n<div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-9d29c028\" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\"><span class=\"uagb-icon uagb-faq-icon-wrap\"><svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\"><path d=\"M432 256c0 17.69-14.33 32.01-32 32.01H256v144c0 17.69-14.33 31.99-32 31.99s-32-14.3-32-31.99v-144H48c-17.67 0-32-14.32-32-32.01s14.33-31.99 32-31.99H192v-144c0-17.69 14.33-32.01 32-32.01s32 14.32 32 32.01v144h144C417.7 224 432 238.3 432 256z\"><\/path><\/svg><\/span><span class=\"uagb-icon-active uagb-faq-icon-wrap\"><svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\"><path d=\"M400 288h-352c-17.69 0-32-14.32-32-32.01s14.31-31.99 32-31.99h352c17.69 0 32 14.3 32 31.99S417.7 288 400 288z\"><\/path><\/svg><\/span><span class=\"uagb-question\">Problem<\/span><\/div><div class=\"uagb-faq-content\"><p>Troubleshooting workflow.<\/p><\/div><\/div>\n<\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Leica TCS SP8 is a high-end inverted confocal microscope designed for advanced fluorescence imaging of fixed and live biological samples. It offers outstanding sensitivity, resolution, and versatility suitable for various applications, including cell biology, developmental biology, neurobiology, plant sciences, and more. Manufacturer: Leica MicrosystemsModel: Leica TCS SP8 (inverted)Location: room S39Phone: +420 22195 1943 Technical [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":6414,"parent":5507,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-6413","page","type-page","status-publish","has-post-thumbnail","hentry"],"uagb_featured_image_src":{"full":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg",898,897,false],"thumbnail":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8-150x150.jpg",150,150,true],"medium":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8-300x300.jpg",300,300,true],"medium_large":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8-768x767.jpg",640,639,true],"large":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg",640,639,false],"1536x1536":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg",898,897,false],"2048x2048":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg",898,897,false],"trp-custom-language-flag":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8-12x12.jpg",12,12,true],"wpsso-schema-1x1":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg",898,897,false],"wpsso-schema-4x3":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg",898,897,false],"wpsso-schema-16x9":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg",898,897,false],"wpsso-thumbnail":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg",898,897,false],"wpsso-opengraph":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg",898,897,false],"wpsso-tc-summary":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg",898,897,false],"wpsso-tc-lrgimg":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Confocal-SP8.jpg",898,897,false]},"uagb_author_info":{"display_name":"Ond\u0159ej \u0160ebesta","author_link":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/author\/schebique\/"},"uagb_comment_info":0,"uagb_excerpt":"The Leica TCS SP8 is a high-end inverted confocal microscope designed for advanced fluorescence imaging of fixed and live biological samples. It offers outstanding sensitivity, resolution, and versatility suitable for various applications, including cell biology, developmental biology, neurobiology, plant sciences, and more. Manufacturer: Leica MicrosystemsModel: Leica TCS SP8 (inverted)Location: room S39Phone: +420 22195 1943 Technical&hellip;","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/6413","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/comments?post=6413"}],"version-history":[{"count":17,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/6413\/revisions"}],"predecessor-version":[{"id":7023,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/6413\/revisions\/7023"}],"up":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/5507"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/media\/6414"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/media?parent=6413"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}