{"id":6449,"date":"2025-03-24T16:51:49","date_gmt":"2025-03-24T14:51:49","guid":{"rendered":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/?page_id=6449"},"modified":"2026-02-23T14:44:53","modified_gmt":"2026-02-23T12:44:53","slug":"nikon-tie2","status":"publish","type":"page","link":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/konfmi\/nikon-tie2\/","title":{"rendered":"Nikon TiE2"},"content":{"rendered":"\n<div class=\"wp-block-media-text is-stacked-on-mobile\"><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"990\" src=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Nikon-TiE2-1024x990.jpg\" alt=\"\" class=\"wp-image-6450 size-full\" srcset=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Nikon-TiE2-1024x990.jpg 1024w, https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Nikon-TiE2-300x290.jpg 300w, https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Nikon-TiE2-768x742.jpg 768w, https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Nikon-TiE2-12x12.jpg 12w, https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2025\/03\/Nikon-TiE2.jpg 1200w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure><div class=\"wp-block-media-text__content\">\n<p class=\"wp-block-paragraph\">The <strong>Nikon ECLIPSE Ti2<\/strong> is an advanced inverted research microscope renowned for its exceptional optical performance, expansive 25mm field of view (FOV), and versatility in accommodating a wide range of imaging applications, from routine cell culture observations to complex live-cell imaging studies using advanced JOBs module.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Manufacturer:<\/strong> <a href=\"https:\/\/www.microscope.healthcare.nikon.com\" data-type=\"link\" data-id=\"https:\/\/www.leica-microsystems.com\/\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">Nikon Instruments Inc.<\/a><br><strong>Model:<\/strong> Nikon ECLIPSE Ti2-E<br><strong>Location:<\/strong> room p108 (floor)<br><strong>Phone: <\/strong>+420 22195 1033<\/p>\n<\/div><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Technical Specifications<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Imaging Technology<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Microscope Type:<\/strong> Inverted laser scanning confocal microscope<\/li>\n\n\n\n<li><strong>Illumination:<\/strong>\n<ul class=\"wp-block-list\">\n<li>LED illumination for both diascopic imaging<\/li>\n\n\n\n<li><a href=\"https:\/\/www.coolled.com\/products\/pe-4000\/\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">CoolLED pe-4000<\/a> illumination for epi-fluorescence imaging<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Detectors:<\/strong>\n<ul class=\"wp-block-list\">\n<li>Monochromatic <a href=\"https:\/\/www.hamamatsu.com\/eu\/en\/product\/cameras\/cmos-cameras\/C13440-20CU.html\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">Hamamatsu ORCA Flash 4.0 V3<\/a> (C13440-20CU)<\/li>\n\n\n\n<li>Color camera TIS<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Objectives Available:<\/strong>\n<ul class=\"wp-block-list\">\n<li>4x\/0.13 WD 16500 \u03bcm (air)<\/li>\n\n\n\n<li>10x\/0.3 WD 16000 \u03bcm (air)<\/li>\n\n\n\n<li>20x\/0.5 WD 2100 \u03bcm (air)<\/li>\n\n\n\n<li>40x\/0.75 WD 660 \u03bcm (air)<\/li>\n\n\n\n<li>60x\/1.4 WD 130 \u03bcm (high NA Oil immersion)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Advanced Imaging Capabilities<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Multi-channel imaging<\/li>\n\n\n\n<li>Z-stacks and 3D reconstruction<\/li>\n\n\n\n<li>Fast Time-lapse imaging (up to 100 fps full-frame)<\/li>\n\n\n\n<li>Multiposition and Tile acquisition<\/li>\n\n\n\n<li>High Throughput Imaging with JOBs module<\/li>\n\n\n\n<li>IRM (Inverted Reflectance Microscopy) <\/li>\n\n\n\n<li>Highly customizable workflow<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Live-Cell Imaging Capabilities<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>OKO-LAB environmental chamber:\n<ul class=\"wp-block-list\">\n<li>Temperature control (from 3\u00b0C above ambient to 60\u00b0C)<\/li>\n\n\n\n<li>CO\u2082 (0-18%) concentration control for maintaining physiological conditions<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">User Information<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Access Requirements<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Mandatory <a href=\"\/sekce-bi\/VMCF\/vmcf-user-registration-guide\/\">user registration<\/a><\/li>\n\n\n\n<li>Facility-provided <a href=\"https:\/\/calpendo.natur.cuni.cz\/program.html#availableTrainings\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">training<\/a> is required before independent use<\/li>\n\n\n\n<li>Users must understand and demonstrate the correct setup of <a href=\"https:\/\/www.microscopyu.com\/tutorials\/kohler\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">K\u00f6hler illumination<\/a> for independent microscope operation<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Booking and Usage Policies<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Reservation via <a href=\"https:\/\/calpendo.natur.cuni.cz\/\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">online booking system<\/a> after completing registration and training<\/li>\n\n\n\n<li>Booking guidelines and facility usage rules provided during training<\/li>\n<\/ul>\n\n\n\n<iframe loading=\"lazy\" \n  src=\"https:\/\/calpendo.natur.cuni.cz\/anon\/WeeklyCalendar.html?title=Confocals%20Weekly&#038;pks=20231003.104213.0.326\" \n  width=\"100%\" \n  height=\"600\" \n  style=\"border:0;\"\n  title=\"Confocals Weekly Calendar\"\n  allowfullscreen>\n<\/iframe>\n\n\n\n<h3 class=\"wp-block-heading\">Price<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Pricing for individual services is determined by the <a href=\"\/sekce-bi\/VMCF\/konfmi\/price-list\/\">current price list<\/a>. Should you have specific questions regarding the pricing of your project, please feel free to <a href=\"\/sekce-bi\/VMCF\/konfmi\/konfmi-our-team\/\">contact<\/a> us.<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<div class=\"wp-block-uagb-container uagb-block-3656525b alignfull uagb-is-root-container\"><div class=\"uagb-container-inner-blocks-wrap\">\n<h2 class=\"wp-block-heading\">Troubleshooting<\/h2>\n\n\n\n<div class=\"wp-block-uagb-faq uagb-faq__outer-wrap uagb-block-3cb66ab7 uagb-faq-icon-row uagb-faq-layout-accordion uagb-faq-expand-first-false uagb-faq-inactive-other-true uagb-faq__wrap uagb-buttons-layout-wrap uagb-faq-equal-height\" data-faqtoggle=\"false\" role=\"tablist\">\n<div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-9d29c028\" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\"><span class=\"uagb-icon uagb-faq-icon-wrap\"><svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\"><path d=\"M432 256c0 17.69-14.33 32.01-32 32.01H256v144c0 17.69-14.33 31.99-32 31.99s-32-14.3-32-31.99v-144H48c-17.67 0-32-14.32-32-32.01s14.33-31.99 32-31.99H192v-144c0-17.69 14.33-32.01 32-32.01s32 14.32 32 32.01v144h144C417.7 224 432 238.3 432 256z\"><\/path><\/svg><\/span><span class=\"uagb-icon-active uagb-faq-icon-wrap\"><svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\"><path d=\"M400 288h-352c-17.69 0-32-14.32-32-32.01s14.31-31.99 32-31.99h352c17.69 0 32 14.3 32 31.99S417.7 288 400 288z\"><\/path><\/svg><\/span><span class=\"uagb-question\">Problem<\/span><\/div><div class=\"uagb-faq-content\"><p>Troubleshooting workflow.<\/p><\/div><\/div>\n<\/div>\n<\/div><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Nikon ECLIPSE Ti2 is an advanced inverted research microscope renowned for its exceptional optical performance, expansive 25mm field of view (FOV), and versatility in accommodating a wide range of imaging applications, from routine cell culture observations to complex live-cell imaging studies using advanced JOBs module. Manufacturer: Nikon Instruments Inc.Model: Nikon ECLIPSE Ti2-ELocation: room p108 [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":5507,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-6449","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"wpsso-schema-1x1":false,"wpsso-schema-4x3":false,"wpsso-schema-16x9":false,"wpsso-thumbnail":false,"wpsso-opengraph":false,"wpsso-tc-summary":false,"wpsso-tc-lrgimg":false},"uagb_author_info":{"display_name":"Ond\u0159ej \u0160ebesta","author_link":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/author\/schebique\/"},"uagb_comment_info":0,"uagb_excerpt":"The Nikon ECLIPSE Ti2 is an advanced inverted research microscope renowned for its exceptional optical performance, expansive 25mm field of view (FOV), and versatility in accommodating a wide range of imaging applications, from routine cell culture observations to complex live-cell imaging studies using advanced JOBs module. Manufacturer: Nikon Instruments Inc.Model: Nikon ECLIPSE Ti2-ELocation: room p108&hellip;","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/6449","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/comments?post=6449"}],"version-history":[{"count":12,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/6449\/revisions"}],"predecessor-version":[{"id":6962,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/6449\/revisions\/6962"}],"up":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/5507"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/media?parent=6449"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}