{"id":6947,"date":"2026-02-23T14:29:30","date_gmt":"2026-02-23T12:29:30","guid":{"rendered":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/?page_id=6947"},"modified":"2026-03-10T16:01:42","modified_gmt":"2026-03-10T14:01:42","slug":"raman","status":"publish","type":"page","link":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/konfmi\/raman\/","title":{"rendered":"WITec Alpha300 Ri (Raman)"},"content":{"rendered":"\n<div class=\"wp-block-media-text is-stacked-on-mobile is-vertically-aligned-top\"><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"454\" height=\"605\" src=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png\" alt=\"\" class=\"wp-image-6948 size-full\" srcset=\"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png 454w, https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope-9x12.png 9w, https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope-225x300.png 225w\" sizes=\"auto, (max-width: 454px) 100vw, 454px\" \/><\/figure><div class=\"wp-block-media-text__content\">\n<p class=\"wp-block-paragraph\"><br>The <strong>inverted confocal Raman microscope WITec Alpha300 Ri<\/strong> is equipped with a<br><strong>532 nm laser<\/strong> and a highly sensitive Andor detection system, designed for confocal micro-Raman spectroscopy in the visible spectral range. Signal detection is based on a cooled CCD camera optimized for the<br><strong>UV\u2013VIS<\/strong> region, providing very high detection efficiency already around 400 nm, which enables reliable acquisition of even very weak Raman signals.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>The microscope was co-financed as part of the project &#8220;Development of Infrastructure for Doctoral Programs at Charles University&#8221;<\/strong> with registration number CZ.02.01.01\/00\/22_012\/0005514 <strong>and is intended for teaching and student training in modern optical and spectroscopic methods only!<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Manufacturer:<\/strong> <a href=\"https:\/\/raman.oxinst.de\/\" data-type=\"link\" data-id=\"https:\/\/www.leica-microsystems.com\/\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">OXFORD Instruments<\/a><br><strong>Model:<\/strong> <strong>WITec Alpha300 Ri<\/strong> (inverted)<br><strong>Location:<\/strong> room S47<br><strong>Phone: <\/strong>+420 22195 1061<\/p>\n<\/div><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Technical Specifications<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Imaging Technology<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Microscope Type:<\/strong> Inverted confocal Raman microscope<\/li>\n\n\n\n<li><strong>Microscope Stand: <\/strong>Nikon ECLIPSE Ti2 (Manufacturer: <a href=\"https:\/\/www.microscope.healthcare.nikon.com\/en_EU\/products\/inverted-microscopes\/eclipse-ti2-series\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">Nikon Instruments Inc.<\/a>)<\/li>\n\n\n\n<li><strong>Lasers Available:<\/strong>\n<ul class=\"wp-block-list\">\n<li>532 nm \/ 30 mW laser for confocal micro-Raman spectroscopy in the visible spectral range<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Detectors:<\/strong>\n<ul class=\"wp-block-list\">\n<li>Cooled <a href=\"https:\/\/www.theimagingsource.com\/en-us\/product\/industrial\/33g\/dfk33gx178\/\">ImagingSource WOI1-33GX178 CMOS<\/a> camera optimized for the UV\u2013VIS region (high efficiency from ~400 nm).<\/li>\n\n\n\n<li>The <strong><a href=\"https:\/\/www.google.com\/url?sa=t&amp;source=web&amp;rct=j&amp;opi=89978449&amp;url=https:\/\/raman.oxinst.de\/assets\/uploads\/raman\/materials\/WITec-Spectroscopy-Solutions-Brochure.pdf&amp;ved=2ahUKEwjiy4fRsu-SAxUcg_0HHeB2GvoQFnoECAoQAQ&amp;usg=AOvVaw2EdHi6TgmVlEB9czySnN6B\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">WITec UHTS 300 VIS<\/a><\/strong> is a high-throughput (f\/4 aperture) lens-based spectrometer with a 300 mm focal length, designed for ultra-fast, high-sensitivity Raman imaging and spectroscopy paired with an <strong><a href=\"https:\/\/andor.oxinst.com\/products\/idus-spectroscopy-cameras\/idus-401\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">Andor iDus CCD camera<\/a><\/strong><\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Objectives Available:<\/strong>\n<ul class=\"wp-block-list\">\n<li>TU PLAN FLUOR 5x\/0.15 WD 2350 \u03bcm (air)<\/li>\n\n\n\n<li>PLAN FLUOR 40x\/0.75 WD 660 \u03bcm (air)<\/li>\n\n\n\n<li>TU PLAN FLUOR 50x\/0.8 WD 1000 \u03bcm (air)<\/li>\n\n\n\n<li>TU PLAN FLUOR 100x\/0.9 WD 1000 \u03bcm (air)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Advanced Imaging Capabilities<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li> <strong>Raman Mapping:<\/strong> Rapid determination of chemical composition for each pixel in the image<\/li>\n\n\n\n<li> <strong>Spectral Imaging:<\/strong> Pixel-wise acquisition of fluorescence spectra<\/li>\n\n\n\n<li> <strong>Multi-modal Correlation:<\/strong> Direct correlation of reflectance and absorbance measurements with Raman chemical maps<\/li>\n\n\n\n<li> <strong>High-Resolution Analysis:<\/strong> Label-free chemical analysis with spatial resolution at the diffraction limit of light microscopy<\/li>\n\n\n\n<li> <strong>Confocal Z-sampling:<\/strong> High-quality confocal design allows Raman signal collection from a precisely defined sample volume only<\/li>\n\n\n\n<li> <strong>Sample Protection:<\/strong> Optimized for low laser power to reduce local heating, photobleaching, or damage to biological samples<\/li>\n\n\n\n<li> <strong>Wide-field Navigation:<\/strong> Wide field of view (25 mm FOV) and overview imaging for efficient sample navigation and selection of measurement regions<\/li>\n\n\n\n<li><strong>Fluorescence:<\/strong> Basic fluorescence for DAPI, FITC, TRITC imaging<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Sample preparation<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">under construction<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Live-Cell Imaging Capabilities <em>(optionally, not tested)<\/em><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>OKO-LAB Bold Line environmental chamber (optionally, not tested):\n<ul class=\"wp-block-list\">\n<li>Temperature control (-5\u00b0C &#8211; 60\u00b0C)<\/li>\n\n\n\n<li>O<sub>2<\/sub> (0-20%) \/ CO\u2082 (1-95%) concentration control for maintaining physiological conditions<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">User Information<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Access Requirements<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Mandatory <a href=\"\/sekce-bi\/VMCF\/vmcf-user-registration-guide\/\">user registration<\/a><\/li>\n\n\n\n<li>Facility-provided <a href=\"https:\/\/calpendo.natur.cuni.cz\/program.html#availableTrainings\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">training<\/a> is required before independent use<\/li>\n\n\n\n<li>Users must understand and demonstrate the correct setup of <a href=\"https:\/\/www.microscopyu.com\/tutorials\/kohler\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">K\u00f6hler illumination<\/a> for independent microscope operation<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Booking and Usage Policies<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Reservation via the <a href=\"https:\/\/calpendo.natur.cuni.cz\/\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">online booking system<\/a> after completing registration<\/li>\n\n\n\n<li>The system can be used only under the operator&#8217;s supervision.<\/li>\n\n\n\n<li>Booking guidelines and facility usage rules were provided during training<\/li>\n<\/ul>\n\n\n\n<iframe loading=\"lazy\" \n  src=\"https:\/\/calpendo.natur.cuni.cz\/anon\/WeeklyCalendar.html?title=Confocals%20Weekly&#038;pks=20251103.122139.2.326\" \n  width=\"100%\" \n  height=\"600\" \n  style=\"border:0;\"\n  title=\"Confocals Weekly Calendar\"\n  allowfullscreen>\n<\/iframe>\n\n\n\n<h3 class=\"wp-block-heading\">Price<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Microscope is available free of charge for students (coursework, thesis work) and academic staff (teaching purposes only). Contact us for more details.<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<div class=\"wp-block-uagb-container uagb-block-3656525b alignfull uagb-is-root-container\"><div class=\"uagb-container-inner-blocks-wrap\">\n<h2 class=\"wp-block-heading\">Troubleshooting<\/h2>\n\n\n\n<div class=\"wp-block-uagb-faq uagb-faq__outer-wrap uagb-block-3cb66ab7 uagb-faq-icon-row uagb-faq-layout-accordion uagb-faq-expand-first-false uagb-faq-inactive-other-true uagb-faq__wrap uagb-buttons-layout-wrap uagb-faq-equal-height\" data-faqtoggle=\"false\" role=\"tablist\">\n<div class=\"wp-block-uagb-faq-child uagb-faq-child__outer-wrap uagb-faq-item uagb-block-9d29c028\" role=\"tab\" tabindex=\"0\"><div class=\"uagb-faq-questions-button uagb-faq-questions\"><span class=\"uagb-icon uagb-faq-icon-wrap\"><svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\"><path d=\"M432 256c0 17.69-14.33 32.01-32 32.01H256v144c0 17.69-14.33 31.99-32 31.99s-32-14.3-32-31.99v-144H48c-17.67 0-32-14.32-32-32.01s14.33-31.99 32-31.99H192v-144c0-17.69 14.33-32.01 32-32.01s32 14.32 32 32.01v144h144C417.7 224 432 238.3 432 256z\"><\/path><\/svg><\/span><span class=\"uagb-icon-active uagb-faq-icon-wrap\"><svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\"><path d=\"M400 288h-352c-17.69 0-32-14.32-32-32.01s14.31-31.99 32-31.99h352c17.69 0 32 14.3 32 31.99S417.7 288 400 288z\"><\/path><\/svg><\/span><span class=\"uagb-question\">Problem<\/span><\/div><div class=\"uagb-faq-content\"><p>Troubleshooting workflow.<\/p><\/div><\/div>\n<\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The inverted confocal Raman microscope WITec Alpha300 Ri is equipped with a532 nm laser and a highly sensitive Andor detection system, designed for confocal micro-Raman spectroscopy in the visible spectral range. Signal detection is based on a cooled CCD camera optimized for theUV\u2013VIS region, providing very high detection efficiency already around 400 nm, which enables [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":6948,"parent":5507,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-6947","page","type-page","status-publish","has-post-thumbnail","hentry"],"uagb_featured_image_src":{"full":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png",454,605,false],"thumbnail":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope-150x150.png",150,150,true],"medium":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope-225x300.png",225,300,true],"medium_large":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png",454,605,false],"large":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png",454,605,false],"1536x1536":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png",454,605,false],"2048x2048":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png",454,605,false],"trp-custom-language-flag":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope-9x12.png",9,12,true],"wpsso-schema-1x1":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png",454,605,false],"wpsso-schema-4x3":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png",454,605,false],"wpsso-schema-16x9":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png",454,605,false],"wpsso-thumbnail":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png",454,605,false],"wpsso-opengraph":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png",454,605,false],"wpsso-tc-summary":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png",454,605,false],"wpsso-tc-lrgimg":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2026\/02\/Raman_microscope.png",454,605,false]},"uagb_author_info":{"display_name":"Ond\u0159ej \u0160ebesta","author_link":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/author\/schebique\/"},"uagb_comment_info":0,"uagb_excerpt":"The inverted confocal Raman microscope WITec Alpha300 Ri is equipped with a532 nm laser and a highly sensitive Andor detection system, designed for confocal micro-Raman spectroscopy in the visible spectral range. Signal detection is based on a cooled CCD camera optimized for theUV\u2013VIS region, providing very high detection efficiency already around 400 nm, which enables&hellip;","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/6947","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/comments?post=6947"}],"version-history":[{"count":7,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/6947\/revisions"}],"predecessor-version":[{"id":7032,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/6947\/revisions\/7032"}],"up":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/pages\/5507"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/media\/6948"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/media?parent=6947"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}