{"id":5574,"date":"2023-08-10T18:26:46","date_gmt":"2023-08-10T18:26:46","guid":{"rendered":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/?p=5574"},"modified":"2025-03-14T20:59:27","modified_gmt":"2025-03-14T18:59:27","slug":"advanced-methods-of-scanning-electron-microscopy","status":"publish","type":"post","link":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/advanced-methods-of-scanning-electron-microscopy\/","title":{"rendered":"Advanced Methods of Scanning Electron Microscopy"},"content":{"rendered":"\n
\"\"<\/figure>\n\n\n

This course<\/strong> is focused<\/strong> on a theoretical<\/strong> and practical<\/strong> introduction to scanning electron microscopy<\/strong> and its biological applications. The programme covers fundamental principles<\/strong> of SEM<\/strong>, current methods of specimen preparation, including cryo approaches<\/strong>, methods of volume SEM like FIB SEM<\/strong>, SBF SEM<\/strong> or MAT<\/strong>, and SEM applications in immunolocalization<\/strong> or correlative microscopy. The main goal of the practical part is to familiarise participants with microscope operation, image acquisition<\/strong>, data processing<\/strong> and useful tricks and tips<\/strong> in sample preparation. The course is limited to 15 participants<\/strong> including both begining and intermediate SEM users. This course is supported by the program for large research infrastructures of the Ministry of Education, Youth and Sports within the project \u201cNational Infrastructure for Biological and Medical Imaging (Czech-BioImaging)\u201c.<\/p>\n\n\n

SCHEDULE OF THE WORKSHOP<\/span><\/h2>\n\n\n

Day 1<\/strong><\/h2>\n

(Location: VMCF Vini\u010dn\u00e1, Microscopy Center, Vini\u010dn\u00e1 7, Praha 2)<\/p>\n

\u00a0<\/p>\n

10:00 – 10:10\u00a0 <\/strong>Welcome Address (Miroslav Hyli\u0161, Faculty of Science, Charles University, Praha)<\/p>\n

10:00 – 10:55\u00a0 <\/strong>Principles of Scanning Electron Microscopy (Vladislav Krzy\u017e\u00e1nek, Institute of Scientific Instruments, CAS, Brno)<\/p>\n

10:55 – 12:00\u00a0 <\/strong>Overview of SEM Sample Preparation Methods at RT and Cryo- conditions (Jana Nebes\u00e1\u0159ov\u00e1, Faculty of Science, Charles University, Praha)<\/p>\n

12:00 – 13:00\u00a0 <\/strong>Lunch<\/p>\n

13:00 –\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong>Practicals<\/p>\n

\u00a0<\/p>\n

Day 2<\/strong><\/h2>\n

(Location: VMCF Vini\u010dn\u00e1, Microscopy Center, Vini\u010dn\u00e1 7, Praha 2)<\/p>\n

\u00a0<\/p>\n

09:00 – 10:15<\/strong>\u00a0 SBF SEM and Micro Array Tomography (Ji\u0159\u00ed T\u00fd\u010d, Biology Centre, CAS, \u010cesk\u00e9 Bud\u011bjovice)<\/p>\n

10:15 – 11:00\u00a0 <\/strong>FIB SEM in Biology Research (Mark\u00e9ta Daleck\u00e1, BIOCEV, Vestec)<\/p>\n

11:00 – 11:30\u00a0 <\/strong>Coffee break<\/p>\n

11:30 – 12:00\u00a0 <\/strong>The Use of SEM in correlative microscopy (Marie Vancov\u00e1, Biology Centre, CAS, \u010cesk\u00e9 Bud\u011bjovice)<\/p>\n

12:00 – 13:00<\/strong>\u00a0 Lunch<\/p>\n

13:00 – 18:30\u00a0 <\/strong>Practicals<\/p>\n

18:30 –\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong>Course Dinner<\/p>\n

\u00a0<\/p>\n

Day 3<\/strong><\/h2>\n

(Location: VMCF Vini\u010dn\u00e1, Microscopy Center, Vini\u010dn\u00e1 7, Praha 2)<\/p>\n

\u00a0<\/p>\n

09:00 – 10:00<\/strong>\u00a0 Introduction in Image Processing (Martin Sch\u00e4tz, Faculty of Science, Charles Univers\u00edty , Praha)<\/p>\n

10:00 – 11:00<\/strong>\u00a0 How to do 3D reconstruction from SEM images? (Ji\u0159\u00ed T\u00fd\u010d, Franti\u0161ek Kitzberger, Biology Centre, CAS, \u010cesk\u00e9 Bud\u011bjovice)<\/p>\n

11:00 – 11:30\u00a0 <\/strong>Coffee break<\/p>\n

11:30 – 12:00\u00a0 <\/strong>Methods of immunolocalization in SEM (Jana Nebes\u00e1\u0159ov\u00e1, Faculty of Science, Charles Univers\u00edty, Praha)<\/p>\n

12:00 – 13:00\u00a0 <\/strong>Lunch<\/p>\n

13:00 – 16:30\u00a0 <\/strong>Practicals<\/p>\n

16:30<\/strong> \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 The end of the course<\/p>\n

\u00a0<\/p>\n\n\n

\n\n\n

Registration fees:<\/h2>\n

CSMS member: 4000 CZK<\/p>\n

CSMS non-member: 5000 CZK<\/p>\n

Industry: 8000 CZK<\/p>\n

The course fee includes admission, course materials, lunches and coffee breaks<\/p>\n\n\n

\n
REGISTRATION<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

This course is focused on a theoretical and practical introduction to scanning electron microscopy and its biological applications. The programme covers fundamental principles of SEM, current methods of specimen preparation, including cryo approaches, methods of volume SEM like FIB SEM, SBF SEM or MAT, and SEM applications in immunolocalization or correlative microscopy. The main goal […]<\/p>\n","protected":false},"author":3,"featured_media":5580,"comment_status":"open","ping_status":"open","sticky":true,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[24,22],"tags":[],"class_list":["post-5574","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-courses","category-workshops"],"uagb_featured_image_src":{"full":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1.png",1684,836,false],"thumbnail":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1-150x150.png",150,150,true],"medium":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1-300x149.png",300,149,true],"medium_large":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1-768x381.png",640,318,true],"large":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1-1024x508.png",640,318,true],"1536x1536":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1-1536x763.png",1536,763,true],"2048x2048":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1.png",1684,836,false],"trp-custom-language-flag":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1.png",18,9,false],"wpsso-schema-1x1":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1.png",1684,836,false],"wpsso-schema-4x3":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1-1200x836.png",1200,836,true],"wpsso-schema-16x9":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1-1200x675-cropped.png",1200,675,true],"wpsso-thumbnail":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1-1200x628-cropped.png",1200,628,true],"wpsso-opengraph":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1-1200x628-cropped.png",1200,628,true],"wpsso-tc-summary":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1.png",1684,836,false],"wpsso-tc-lrgimg":["https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-content\/uploads\/2023\/08\/AMoSEM-1-1200x628-cropped.png",1200,628,true]},"uagb_author_info":{"display_name":"Petra H\u016fle","author_link":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/author\/hulep\/"},"uagb_comment_info":0,"uagb_excerpt":"This course is focused on a theoretical and practical introduction to scanning electron microscopy and its biological applications. The programme covers fundamental principles of SEM, current methods of specimen preparation, including cryo approaches, methods of volume SEM like FIB SEM, SBF SEM or MAT, and SEM applications in immunolocalization or correlative microscopy. The main goal…","_links":{"self":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/posts\/5574","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/comments?post=5574"}],"version-history":[{"count":2,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/posts\/5574\/revisions"}],"predecessor-version":[{"id":5579,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/posts\/5574\/revisions\/5579"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/media\/5580"}],"wp:attachment":[{"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/media?parent=5574"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/categories?post=5574"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.natur.cuni.cz\/sekce-bi\/VMCF\/wp-json\/wp\/v2\/tags?post=5574"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}