Selected Papers
- 2007
- Hlouchová, K., Bařinka, C., Klusák, V., Šácha, P., Mlčochová,
P., Majer, P., Rulíšek, L. and Konvalinka, J. (2007)
Biochemical characterisation of human glutamate carboxypeptidase III.
J. Neurochem. , in press.
- Šácha, P., Zámečník, J., Bařinka, C., Hlouchová, K., Vícha, A.,
Mlčochová, P., Hilgert, I., Eckschlager, T. and Konvalinka, J. (2007)
Expression of Glutamate Carboxypeptidase II in Human Brain.
Neuroscience , in press.
- 2006
- Koval, D., Jirásková, J., Stříšovský, K., Konvalinka, J.
and Kašička, V. (2006)
Capillary electrophoresis method for the determination of
d?serine and its application for monitoring of serine
racemase activity.
Electrophoresis 27, 2558-2566.
- Majerová-Uhlíková, T., Dantuma, N.P., Lindsten, K., Masucci,
M.G. and Konvalinka, J. (2006)
Noninfectious Fluorimetric Assay for Phenotyping of
Drug-Resistant HIV Proteinase Mutants.
J. Clin. Virol. 36, 50-59.
- Mesters, J.R., Bařinka, C., Li, W., Tsukamoto, T., Majer, P.,
Slusher, B., Konvalinka, J. and Hilgenfeld, R. (2006)
Structure of Glutamate Carboxypeptidase II, a Drug Target in
Neuronal Damage and Prostate Cancer.
EMBO J. 25, 1375-1384.
- 2005
- Cígler, P., Kožíšek, M., Řezáčová, P., Otwinowski, Z.,
Pokorná, J., Plešek, J., Grüner, B., Dolečková-Marešová,
L., Máša, M., Bodem, J., Kraeusslich, H.-G., Král, V.
and Konvalinka, J. (2005)
From non-peptide towards non-carbon protease inhibitors:
metallacarboranes as specific and potent inhibitors of HIV
protease.
Proc. Natl. Acad. Sci. USA 102, 15394-15399.
- Stříšovský, K., Jirásková, J., Mikulová, A., Rulíšek, L.
and Konvalinka, J. (2005)
Dual substrate and reaction specificity in mouse serine racemase:
Identification of high-affinity dicarboxylate substrate and
inhibitors and analysis of the ?-eliminase activity.
Biochemistry 44, 13091-13100.
- Václavíková, J., Machala, L., Staňková, M., Linka, M.,
Brůčková, M., Vandasová, J. and Konvalinka, J. (2005)
Response of HIV positive patients to the long-term
salvage therapy by lopinavir/ritonavir.
J. Clin. Virol. 33, 319-323.
- 2004
- Švec, M., Bauerová, H., Pichová, I., Konvalinka, J.
and Stříšovský, K. (2004)
Proteinases of betaretroviruses bind nucleic acids
through a novel interaction module, the G-patch.
FEBS Lett. 576, 271-276.
- Prejdová, J., Souček, M. and Konvalinka, J. (2004)
Determining and Overcoming Resistance to HIV Protease
Inhibitors.
Current Drug Targets -Infect. Disord. 4, 137-152.
- Bařinka, C., Mlčochová, P., Šácha, P., Hilgert, I.,
Majer, P., Slusher, B.S., Hořejší, V. and Konvalinka,
J. (2004)
Amino Acids at N- and C-termini of Human Glutamate
Carboxypeptidase II Are Required for the Enzymatic
Activity and Proper Folding.
Eur. J. Biochem. 271, 2782-2790.
- Bařinka, C., Šácha, P., Sklenář, J., Man, P., Bezouška,
K., Slusher, B.S. and Konvalinka, J. (2004)
Identification of the N-glycosylation Sites on Glutamate
Carboxypeptidase II Necessary for Proteolytic Activity.
Protein Sci. 13, 1627-1635.
- 2003
- Ingr, M., Uhlíková, T., Stříšovský, K., Majerová, E.
and Konvalinka, J. (2003)
Kinetics of the dimerization of retroviral proteases: the
"fireman's grip" and dimerization.
Protein Sci. 12, 2173-2182.
- Skálová, T., Hašek, J., Dohnálek, J., Petroková, H.,
Buchtelová, E., Dušková, J., Souček, M., Majer, P.,
Uhlíková, T. and Konvalinka, J. (2003)
Complex of HIV-1 Protease with Peptidomimetic Inhibitor.
Structural and Energy Analysis of Binding Adaptation
to Mutant and Wild Type Protease.
J. Med. Chem. 46, 1636-1644.
- Stříšovský, K., Jirásková, J., Bařinka, C., Majer, P.,
Rojas, C., Slusher, B.S. and Konvalinka, J. (2003)
Mouse brain serine racemase catalyzes specific
elimination of L-Ser to pyruvate.
FEBS Lett. 535, 44-48.
- 2002
- Weber, J., Mesters, J.R., Lepšík, M., Prejdová, J., Švec, M.,
Šponarová, J., Mlčochová, P., Stříšovský, K., Uhlíková, T.,
Souček, M., Machala, M., Staňková, M., Vondrášek, J., Klimkait, T.,
Kraeusslich, H.-G., Hilgenfeld, R. and Konvalinka, J. (2002)
Unusual binding mode of an HIV-1 protease inhibitor explains its
potency against multi-drug-resistant virus strains.
J. Mol. Biol. 324, 739-754.
- Dohnálek, J., Hašek, J., Dušková, J.,
Petroková, H., Hradilek, M., Souček, M.,
Konvalinka, J., Brynda, J., Sedláček, J. and
Fábry, M. (2002). Hydroxyethylamine Isostere of an
HIV-1 Protease Inhibitor Prefers Its Amine to the Hydroxy
Group in Binding to Catalytic Aspartates. A Synchrotron
Study of HIV-1 Protease in Complex with a Peptidomimetic
Inhibitor.
J. Med. Chem. 45, 1432-1438.
- Stříšovský, K., Smrž, D.,
Fehrmann, F., Kräusslich, H.-G. and Konvalinka, J.
(2002). Murine endogenous retrovirus MIA-14 encodes an
active aspartic proteinase which is functionaly similar to
proteinases from D-type retroviruses.
Arch. Biochem. Biophys. 398, 261-268.
- Bařinka, C., Rinnová, M., Šácha,
P., Rojas, C., Majer, P., Slusher, B.S. and Konvalinka,
J. (2002). Substrate Specificity, Inhibition and
Enzymological Analysis of Recombinant Human Glutamate
Carboxypeptidase II.
J. Neurochem. 80(3), 477-487.
- 2001
- Dohnálek, J., Hašek, J., Dušková,
J., Petroková, H., Hradilek, M., Souček, M.,
Konvalinka, J., Brynda, J., Sedláček, J and
Fábry, M. (2001). A Distinct Binding Mode of a
Hydroxyethylamine Isostere Inhibitor of HIV-1 Protease.
Acta Crystallographica D., D57, 472-476.
- Lindsten, K., Uhlíková, T., Konvalinka, J.,
Masucci, M.G. and Dantuma, N.P. (2001). A cell-based
fluorescence assay for Human Immunodeficiency Virus
(HIV)-1 protease activity.
Antimicrobial Agents Chemotherapy 45,
2616-2622.
- 2000
- Ingr, M. and Konvalinka, J. (2000). Theoretical
description of the direct exponential amplification a
sequencing (DEXAS) method.
Biol. Chem. 381, 439-445.
- Stříšovský, K. Tessmer, U.,
Langner, J., Konvalinka, J. and Kräusslich, H.-G.
(2000). Systematic mutational analysis of the active-site
threonine of HIV-1 proteinase: re-thinking the 'fireman´s
grip' hypothesis.
Protein Sci. 9, 1631-1641.
- Rinnová, M. Hradilek, M., Bařinka, C., Weber,
J., Souček, M., Vondrášek, J., Klimkait,
T. and Konvalinka, J. (2000). A picomolar inhibitor of
resistant strains of HIV PR identified by a combinatorial
approach.
Arch. Biochem. Biophys. 382, 22-30.
- 1997
- Konvalinka, J., Litera, J., Weber, J.,
Vondrášek, J., Hradilek, M., Souček, M.,
Pichová, I., Majer, P., Štrop, P.,
Sedláček, J., Heuser, A.-M., Kottler, H. and
Kräeusslich, H.-G. (1997). Configurations of
diastereomeric hydroxyethylene isosteres strongly afect
biological activities of a series of specific inhibitors
of human-immunodeficiency-virus proteinase.
Eur. J. Biochem. 250, 559-566.
- 1996 and older
- Uhlíková, T., Konvalinka, J.,
Pichová, I., Souček, M., Kraeusslich, H.-G.
and Vondrášek, J. (1996). A modular approach
to HIV-1 proteinase inhibitor design.
Biochem. Biophys. Res. Commun. 222, 38-43.
- Konvalinka, J., Heuser, A.-M.,
Hrušková-Heidingsfeldová, O., Vogt,
V.M., Sedláček, J., Štrop, P. and
Kraeusslich, H.-G. (1995). Proteolytic Processing of
Particle Associated Retroviral Polyproteins by Homologous
and Heterologous Viral Proteinases.
Eur. J. Biochem. 228, 191-198.
- Konvalinka, J., Litterst, M.A., Welker, R., Rippman, F.,
Heuser, A.-M. and Kräusslich, H.-G. (1995). An Active
Site Mutation in the HIV-1 Proteinase (PR) Causes
Reduced PR Activity and Loss of PR mediated Cytotoxicity
without Apparent Effect on Virus Maturation and
Infectivity.
J. Virol. 69, 7180-7186.
- Konvalinka, J., Löchelt, M., Zentgraf, H., Fluegel,
R., and Kräusslich, H.-G. (1995) Active Foamy Virus
Proteinase is Essential for Virus Infectivity but Not
for Formation of a Pol Polyprotein.
J. Virol. 69, 7264-7268.
- Freund, J., Kellner, R., Konvalinka, J., Wolber, V.,
Kräusslich, H.-G. and Kalbitzer, H.R. (1994). A
possible regulation of negative factor (Nef) activity of
human immunodeficiency virus type 1 by the viral
proteinase.
Eur. J. Biochem. 223, 589-593.
- Majer, P., Urban, J., Gregorová, E., Konvalinka,
J., Novek, P., Stehlíková, J.,
Andreánsky, M., Sedláček, J. and
Štrop, P. (1993). Specificity mapping of HIV-1
protease by reduced bond inhibitors.
Arch. Biochem. Biophys. 304, 1-8.
- Konvalinka, J., Hořejší, M.,
Andreánsky, M., Novek, P., Pichová, I.,
Bláha, I., Fábry, M., Sedláček,
J., Foundling, S. and Štrop, P. (1992). A retroviral
proteinase from myeloblastosis associated virus acquires
pH dependence and substrate specificity of the HIV-1
proteinase.
EMBO J. 11, 1141-1144.
- Urban, J., Konvalinka, J.,
Stehlíková, J., Gregorová, E., Majer,
P., Souček, M., Andreánsky, M., Fábry,
M. and Štrop, P. (1992). Reduced-bond tight-binding
inhibitors of HIV-1 protease: fine tuning of the enzyme
subsite specificity.
FEBS. Lett. 298, 9-13.
- Konvalinka, J., Bláha, I., Škrabana, R.,
Sedláček, J., Pichová, I.,
Kaprálek, F., Kostka, V. and Štrop, P. (1991)
Subsite specificity of the proteinase from myeloblastosis
associated virus.
FEBS Lett. 282, 73-76.
- Richards, A.D., Phylip, L.H., Farmerie, W.G., Scarborough, P.E., Alvarez, A., Dunn, B.M., Herve-Hirel, Ph.,
Konvalinka, J., Štrop, P., Pavlíčkova,
L., Kostka, V. and Kay, J. (1990). Sensitive, soluble
chromogenic substrates for HIV-1 proteinase.
J.Biol. Chem. 265, 7733-7736.
- Konvalinka, J., Štrop, P., Velek, J.,
Černá, V., Kostka, V., Phylip, L.-H.,
Richards, A.-D., Dunn, B.-M. and Kay, J. (1990). Sub-site
preferences of the aspartic proteinase from the human
immunodeficiency virus, HIV-1.
FEBS Lett. 268, 35-38.