Dvořák J., Vaňáčová Š., Hampl V., Flegr J., Horák P. (2002): Comparison of European Trichobilharzia species based on ITS1 and ITS2 sequences. Parasitology 124: 307-313.
ISSN: 0031-1820
IF 2.114
Abstract:
Schistosomes are parasites of considerable medical and veterinary importance
and, therefore, all aspects of their biology have been intensively studied.
In contrast, our knowledge of species of the largest genus, Trichobilharzia,
is insufficient. Because morphological characterization of Trichobilharzia species
provides a limited number of criteria for species determination, molecular data
are required. In the present paper, we sequenced internal transcribed spacers
ITS1 and ITS2, and 5.8S ribosomal RNA (rRNA) genes of 3 European Trichobilharzia
species (T. regenti, T. szidati and T. franki). We showed that ITS1 and ITS2
sequences can be used in species identification. Repetitive elements were found
in ITS I of all 3 Trichobilharzia species; their number and length varied depending
on the species. Phylogenetic analysis showed that the visceral T. franki is
more related to the nasal T. regenti, than to the visceral T. szidati. The newly
designed primer, which is specific for T. regenti, might be used as a tool for
diagnosis of this potential pathogen.
Author Keywords:
Trichobilharzia; schistosomes; ribosomal DNA; repeats; recombinational hot-spot
site; chi-like site
KeyWords Plus:
INTERNAL TRANSCRIBED SPACER; REPETITIVE SEQUENCES; RIBOSOMAL DNA; LIFE-CYCLE;
SCHISTOSOMA; DIGENEA; REGENTI