Šedinová J., Flegr J., Ey P., Kulda J. 2002: Use of random polymorphic DNA (RAPD) analysis for the identification of Giardia intestinalis subtypes. Abstrakt, J.Eukaryotic Microbiology, 49, 9A.

ISSN 1066-5234,

IF 1,739

ABSTRACT. The random amplified polymorphic DNA (RAPD) method was used to investigate genetic polymorphisms among 25 isolates of Giardia intestinalis and to assess the utility of RAPD for subtype detection and genealogical analysis. Using data obtained for 6 human and 19 animal-derived isolates in polymerase chain reactions with 13 different primers, phylogenetic trees were constructed and bootstrap values computed by the program FreeTree. Three major clades were distinguished, corresponding to previously defined genetic assemblages A, B and E. The purported specificity of assemblage E genotypes for artiodactyl hosts was supported. Assemblages A and B showed wide host spectra, including human and animal hosts. The identity of RAPD patterns obtained from 14 clones derived from one G. intestinalis isolate (P15) indicated that the isolate was genetically homogenous. No correlation was found between the genotype of analysed isolates and the presence or absence of the double-stranded RNA Giardiavirus. The results indicate that RAPD banding data provide reliable genetic information that can be used for both 'fingerprinting' and genealogical purposes.