Practical Course

Dates: Nov 18-21, 2025

BIOCEV, Vestec

Correlative light and electron microscopy (CLEM) leverages on combination of two complementary microscopy approaches. The 3D-CLEM then allows for detection, identification and spatial distribution of features of interest within 3D volume of a sample and for downstream characterization of the features ultrastructure, orientation and co-localization with other structures in the volume. Besides covering principles of the method, this 3D-CLEM workshop will discuss and present a wide range of methodical approaches and protocols to assist users with selection of the optimal experiment workflow, sample preparation, Light & Fluorescence 3D imaging and subsequent sample processing for ultrastructural analysis by 3D volume Electron Microscopy. Concepts of 3D-CLEM imaging data post-processing, correlation, evaluation and troubleshooting will also be included. The demo and practical sessions will be conducted using the state-of-the-art equipment available at the Imaging Methods Core Facility (IMCF-BIOCEV), including Super-resolution fluorescence microscopy, Cryo-fluorescence, Focussed-ion beam SEM and Electron tomography and dedicated software for 3D-imaging data processing and analysis. The workshop is focussed on participants with previous experience in microscopy who are interested in learning advanced correlative imaging workflows and in their application across various experimental models in life sciences.

The course is supported by the National Infrastructure for Biological and Medical Imaging (Czech-BioImaging, Ministry of Education, Youth and Sports – Large Research Infrastructure, LM2023050).