The following lectures are taught by members of the Laboratory of Cancer Cell Invasion at Faculty of Science, Charles University.
Molecular Biology of Cancer II
Jan Brábek, PhD
Daniel Rősel, PhD
Ondřej Tolde, PhD
The lecture provides a more thorough overview of some selected areas of tumor biology, with special emphasis on tumor cell metabolism, tumor microenvironment, migration and invasiveness of tumor cells, including molecular mechanisms. Most lectures contain information on modern methodological approaches to the study of relevant issues.
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The course provides a working knowledge of biochemistry, focusing on practical aspects of the field. It covers basic biochemical techniques used in modern experimental biology, e.g. chromatography, spectrophotometry, and electrophoresis applied to the analyses of lipids, carbohydrates, proteins and enzymes in various biological materials. Students are given the opportunity to perform simple biological experiments independently. Examples of experiments: 1) Determination of glycogen levels in the mouse liver after starvation. 2) Isolation of recombinant protein, SDS-PAGE electrophoresis. 3) Quantitative analysis of erythrocytes phospholipids in ruminant and non-ruminant mammals.
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The first part aims to introduce the various groups of biologically important molecules whose knowledge is needed to study cellular metabolism. The second part is focused on protein structures and their functions with emphasis on enzymology. The third part presents the key pathways of catabolism and cell anabolism with emphasis on the animal cell. Metabolism is discussed along with its regulatory circuits.
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Physiology of the Cell
Daniel Rősel, PhD
The course is focused on general properties of eukaryotic cells, their structure and functional compartmentalisation. The first part is dedicated to bioenergetics : the general principles of energy transduction in living cells, the sources of energy, the main energy producing a consuming pathways, their localization within the framework of a single cell, and, finally, the interrelation of different pathways and their regulation. The second part is dedicated to important cellular structures and their roles in keeping the cell in fully functional state : biomembranes (structure, role, membrane transport), cellular organelles (endoplasmatic reticulum, Golgi apparatus, peroxisomes, semiautonomic organelles and others) and cytoskeleton.