Laboratory of Structural Biochemistry of Immune Recognition

Faculty of Science, Charles University

Curriculum vitae – MSc. Ondřej Skořepa, Ph.D.

Postdoctoral fellow at Max Planck Institute of Biochemistry, Martinsried, Germany

Research profiles
ResearchGate
Google Scholar
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e-mail: skorepao@natur.cuni.cz
Teaching

Advanced Practical Course in Biochemistry I (CZ/EN)

Introduction to Knowledge and Technology Transfer

Education

2024 – now

Post-doctoral internship
Molecular Imaging and Bionanotechnology

Max Planck Institute of Biochemistry, Martinsried, Germany
Ralf Jungmann Group

2024 – now
2021 – 2023

Post-doctoral internship
Biochemistry

Charles University, Faculty of Science

2021 – 2023
2016 – 2021

Doctoral programme
Biochemistry

Charles University, Faculty of Science

2016 – 2021
2014 – 2016

Master programme
Clinical and Toxicological Analysis

Charles University, Faculty of Science

2014 – 2016
2010 – 2014

Bachelor programme
Clinical and Toxicological Analysis

Charles University, Faculty of Science

2010 – 2014

Language skills

Czech – mother tongue
English – C1, CAE certificate

Publication

2022Bláha J., Skálová T., Kalousková B., Skořepa O., Cmunt D., Grobárová V., Pazicky S., Poláchová E., Abreu C., Stránský J., Kovaľ T., Dušková J., Zhao Y., Harlos K., Hašek J., Dohnálek J., Vaněk O. (2022): Structure of the human NK cell NKR-P1:LLT1 receptor:ligand complex reveals clustering in the immune synapse. Nat Commun 13, 5022. IF 17.694 DOI: 10.1038/s41467-022-32577-6
2022Vaněk O., Kalousková B., Abreu C., Nejadebrahim S., Skořepa O. (2022): Natural killer cell-based strategies for immunotherapy of cancer. Adv Protein Chem Struct Biol 129, 91-133. IF 5.447 DOI: 10.1016/bs.apcsb.2022.02.001
2021Peleg Y., Vincentelli R., Collins B. M., Chen K., Livingstone E. K., Weeratunga S., Leneva N., Guo Q., Remans K., Perez K., Bjerga G. E. K., Larsen Ø., Vaněk O., Skořepa O., Jacquemin S., Poterszman A., Kjaer S., Christodoulou E., Albeck S., Dym O., Ainbinder E., Unger T., Schuetz A., Matthes S., Bader M., de Marco A., Storici P., Semrau M. S., Stolt-Bergner P., Aigner C., Suppmann S., Goldenzweig A., Fleishman S. J. (2021): Community-wide experimental evaluation of the PROSS stability-design method. J Mol Biol 433, 166964. IF 6.151 DOI: 10.1016/j.jmb.2021.166964
2021Kalousková B., Skořepa O., Cmunt D., Abreu C., Krejčová K., Bláha J., Sieglová I., Král V., Fábry M., Pola R., Pechar M., Vaněk O. (2021): Tumor marker B7-H6 bound to the coiled coil peptide-polymer conjugate enables targeted therapy by activating human natural killer cells. Biomedicines 9, 1597. IF 4.757 DOI: 10.3390/biomedicines9111597
2020Skořepa O., Pažický S., Kalousková B., Bláha J., Abreu C., Ječmen T., Rosůlek M., Fish A., Sedivy A., Harlos K., Dohnálek J., Skálová T., Vaněk O.* (2020): Natural killer cell activation receptor NKp30 oligomerization depends on its N-glycosylation. Cancers 12, 1998. IF 6.639 DOI: 10.3390/cancers12071998
2019Vaněk O., Celadová P., Skořepa O., Bláha J., Kalousková B., Dvorská A., Poláchová E., Pucholtová H., Kavan D., Pompach P., Hofbauerová K., Kopecký V. Jr., Mesci A., Voigt S., Carlyle J. R. (2019): Production of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells. Sci Rep 9, 17836. IF 3.998  DOI: 10.1038/s41598-019-52114-8
2017Bláha J., Kalousková B., Skořepa O., Pažický S., Novák P., Vaněk O. (2017): High-level expression and purification of soluble form of human natural killer cell receptor NKR-P1 in HEK293S GnTI– cells. Protein Expr Purif 140, 36-43. IF 1.338 DOI: 10.1016/j.pep.2017.07.016

Presentations at scientific conferences

2021EBSA 13TH European Biophysics Conference, Vienna, Austria
2020FEBS/INSTRUCT/ARBRE-MOBIEU HYTHABIO Practical Course, Grenoble, France
2020ARBRE-MOBIEU 4th Plenary meeting, Prague, Czech Republic, Co-organiser
2019British Society for Immunology Congress 2019, Liverpool, GB
2019ARBRE-MOBIEU Plenary Meeting COST action CA15126, Zagreb, Croatia
2018EMBO Practical Course Membrane PEPC1, Hamburg, Germany
2018FEBS Course on Hydrodynamic and Thermodynamic Analysis of Biological Macromolecules and Their Interactions, Co-organiser
2017Structural Mass Spectrometry Workshop, Weizmann institute of science, Rehovot, Israel
2017Instruct Biennial Structural Biology Meeting 2017, Brno, Czech Republic
20172nd iNEXT Annual User Meeting in Brno
2017ARBRE-MOBIEU Porto plenary meeting, Porto, Portugal
2016Helmholtz Training Course on Integrative Structural Biology Braunschweig, Germany
2016FEBS practical course “Advanced Methods in Macromolecular Crystallization VII”, Nové Hrady, Czech Republic
2016XIV Discussions in Structural Molecular Biology in Nové Hrady, Czech Republic
2015XVII. Meeting of Biochemists and Molecular Biologists, Brno, Czech Republic

Selected research projects

2023A New Vision: Innovative Imaging Approaches for Investigating the Spatial Organization of Immune Receptors and Ligands. Czech Science Foundation (24-12553O), postdoc individual fellowship outgoing project, 2024 – 2027, principal investigator
2017SEC-SAXS measurements, Diamond synchrotron, Harwell, UK
2017COST Short Term Scientific Mission, Impact of NKp30 glycosylation and oligomerization on its complex formation and ligand binding – the key features for NK cells activation, Netherlands Cancer Institute, Amsterdam, Netherlands
2016Impact of NKp30 glycosylation and oligomerization on its complex formation – the key features for NK cells activation. Grant Agency of the Charles University (927916), 2016 – 2018, principal investigator

Last updated 10. 1. 2024