Southern blot analysis of DNA fragments encapsidated by VP1 pseudocapsids in yeast (A) and insect (B) cells. a: Ethidium bromide-stained agarose gel; b: Southern blot. Hybridisation performed with in vitro 32P-labelled DNA isolated from VP1 pseudocapsids produced in yeast (A) and insect (B) cells. A: Restriction fragments of yeast chromosomal DNA (1–4) and pWYGL7L-VP1 plasmid (6–8). Non-digested yeast chromosomal DNA (5); DNAs digested with EcoRI (2, 7), PstI (3), XhoI (4), BamHI, NotI (6) and HindIII (8). B: Restriction fragments of insect cell chromosomal DNA (1–3) and baculovirus DNA (4–6). DNAs digested with EcoRI (1, 4), HindIII (2, 5) and PstI (3, 6).