Nenalezli-li jste zde vaši publikaci, požádejte o její přidání na seznam zde.
2026
5891878
2FM3Z7J9
1
https://raw.githubusercontent.com/Schebique/vmcf-konfmi/refs/heads/main/vmcf-web-style.csl
50
date
desc
4983
https://web.natur.cuni.cz/sekce-bi/VMCF/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22EVZJHY7G%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Sch%5Cu00e4tz%20and%20Sugawara%22%2C%22parsedDate%22%3A%222026-06-24%22%2C%22numChildren%22%3A6%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BSch%26%23xE4%3Btz%2C%20M.%3B%20Sugawara%2C%20K.%20%26lt%3Bb%26gt%3BThe%20Reproducibility%20Gap%20in%20Graph%20Neural%20Network%20Workflows%20for%20Cell%20Dynamics%3A%20A%20Checklist-Driven%20Case%20Study%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BJournal%20of%20Microscopy%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2026%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3Bn%5C%2Fa%26lt%3B%5C%2Fi%26gt%3B%20%28n%5C%2Fa%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fjmi.70139%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fjmi.70139%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20reproducibility%20gap%20in%20graph%20neural%20network%20workflows%20for%20cell%20dynamics%3A%20A%20checklist-driven%20case%20study%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22Sch%5Cu00e4tz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ko%22%2C%22lastName%22%3A%22Sugawara%22%7D%5D%2C%22abstractNote%22%3A%22As%20part%20of%20a%20Global%20BioImage%20Analysts%26%23039%3B%20Society%20%28GloBIAS%29%20initiative%2C%20we%20evaluated%20the%20reproducibility%20of%20a%20Graph%20Neural%20Network%20%28GNN%29%20study%20on%20cell%20dynamics%20using%20structured%2C%20community-developed%20checklists%20from%20the%20Quality%20Assessment%20and%20Reproducibility%20for%20Instruments%20and%20Images%20in%20Light%20Microscopy%20%28QUAREP-LiMi%29%20initiative.%20Notably%2C%20these%20checklists%20were%20published%20after%20the%202022%20Target%20Paper%2C%20meaning%20our%20assessment%20is%20necessarily%20retrospective.%20Our%20assessment%20revealed%20a%20gap%20between%20recent%20reporting%20standards%20and%20practical%20execution.%20Reproduction%20attempts%20across%20multiple%20environments%20confirmed%20deficiencies%2C%20including%20the%20absence%20of%20explicit%20image%20metadata%20%28pixel%20size%20and%20timestamps%29%2C%20which%20limits%20quantitative%20interpretation.%20Furthermore%2C%20the%20absence%20of%20a%20software%20container%2C%20which%20was%20typical%20for%20the%20time%20of%20publication%20and%20the%20incomplete%20dependency%20list%2C%20necessitated%20complex%20manual%20environment%20configuration%2C%20increasing%20the%20barrier%20to%20entry.%20This%20experience%20highlights%20the%20contrast%20between%20the%20reproducibility%20expectations%20at%20the%20time%20and%20newer%20standards%20achieved%20by%20minimal%20compliance%20and%20true%20functional%20access.%20We%20conclude%20by%20presenting%20a%20detailed%20discussion%20of%20the%20broader%20implications%20for%20the%20quantitative%20biology%20community%20and%20propose%20actionable%20recommendations%20including%20robust%20environment%20containerisation%20and%20standardised%20data%20deposition%20to%20ensure%20complex%20computational%20workflows%20become%20scientifically%20sound%20and%20reusable%20resources.%20This%20retrospective%20case%20study%20evaluates%20a%20legacy%20computational%20paper%20against%20modern%20reproducibility%20framework%2C%20not%20to%20critique%20past%20non-compliance%2C%20but%20to%20extract%20actionable%20lessons%20for%20future%20research%20standards%22%2C%22date%22%3A%2224.6.2026%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2Fjmi.70139%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fonlinelibrary.wiley.com%5C%2Fdoi%5C%2Fabs%5C%2F10.1111%5C%2Fjmi.70139%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221365-2818%22%2C%22language%22%3A%22en%22%2C%22collections%22%3A%5B%222FM3Z7J9%22%5D%2C%22dateModified%22%3A%222026-07-27T10%3A32%3A25Z%22%7D%7D%2C%7B%22key%22%3A%22SP4YHBWN%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Corbat%20et%20al.%22%2C%22parsedDate%22%3A%222026-04-22%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BCorbat%2C%20A.%20A.%3B%20Walther%2C%20C.%20G.%3B%20de%20la%20Ballina%2C%20L.%20R.%3B%20Condon%2C%20N.%20D.%3B%20Felder%2C%20A.%20A.%3B%20Sch%26%23xE4%3Btz%2C%20M.%3B%20Schmerl%2C%20B.%3B%20Sugawara%2C%20K.%3B%20Prats%2C%20C.%3B%20Klemm%2C%20A.%3B%20Miura%2C%20K.%3B%20Sampaio%2C%20P.%3B%20Tischer%2C%20C.%3B%20Levet%2C%20F.%3B%20D%26%23x2019%3BAntuono%2C%20R.%3B%20Cimini%2C%20B.%20A.%3B%20Haase%2C%20R.%20%26lt%3Bb%26gt%3BGloBIAS%3A%20Strengthening%20the%20Foundations%20of%20Bioimage%20Analysis%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BNat%20Methods%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2026%26lt%3B%5C%2Fb%26gt%3B%2C%201%26%23x2013%3B2.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41592-026-03060-7%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41592-026-03060-7%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22GloBIAS%3A%20strengthening%20the%20foundations%20of%20bioimage%20analysis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Agustin%20A.%22%2C%22lastName%22%3A%22Corbat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christa%20G.%22%2C%22lastName%22%3A%22Walther%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%20R.%22%2C%22lastName%22%3A%22de%20la%20Ballina%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicholas%20D.%22%2C%22lastName%22%3A%22Condon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alessandro%20A.%22%2C%22lastName%22%3A%22Felder%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22Sch%5Cu00e4tz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bettina%22%2C%22lastName%22%3A%22Schmerl%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ko%22%2C%22lastName%22%3A%22Sugawara%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Clara%22%2C%22lastName%22%3A%22Prats%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anna%22%2C%22lastName%22%3A%22Klemm%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kota%22%2C%22lastName%22%3A%22Miura%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paula%22%2C%22lastName%22%3A%22Sampaio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christian%22%2C%22lastName%22%3A%22Tischer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Florian%22%2C%22lastName%22%3A%22Levet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rocco%22%2C%22lastName%22%3A%22D%5Cu2019Antuono%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Beth%20A.%22%2C%22lastName%22%3A%22Cimini%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%22%2C%22lastName%22%3A%22Haase%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222026-04-22%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41592-026-03060-7%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41592-026-03060-7%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221548-7105%22%2C%22language%22%3A%22en%22%2C%22collections%22%3A%5B%222FM3Z7J9%22%5D%2C%22dateModified%22%3A%222026-05-07T11%3A11%3A40Z%22%7D%7D%2C%7B%22key%22%3A%22XF7WB3W2%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Kasik%20et%20al.%22%2C%22parsedDate%22%3A%222026-03-05%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BKasik%2C%20P.%3B%20Galatik%2C%20F.%3B%20Matous%2C%20P.%3B%20Paral%2C%20P.%3B%20Vasek%2C%20D.%3B%20Horn%26%23xED%3Bkova%2C%20D.%3B%20Elsnicova%2C%20B.%3B%20Zurmanova%2C%20J.%20M.%20%26lt%3Bb%26gt%3BCausal%20Relevance%20of%20the%20JAK%5C%2FSTAT%20Pathway%20for%20Cardioprotection%20via%20Cold%20Acclimation%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BSci%20Rep%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2026%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B16%26lt%3B%5C%2Fi%26gt%3B%20%281%29%2C%2012124.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-026-40532-4%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-026-40532-4%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Causal%20relevance%20of%20the%20JAK%5C%2FSTAT%20pathway%20for%20cardioprotection%20via%20cold%20acclimation%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petr%22%2C%22lastName%22%3A%22Kasik%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Frantisek%22%2C%22lastName%22%3A%22Galatik%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petr%22%2C%22lastName%22%3A%22Matous%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petr%22%2C%22lastName%22%3A%22Paral%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%22%2C%22lastName%22%3A%22Vasek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniela%22%2C%22lastName%22%3A%22Horn%5Cu00edkova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Barbara%22%2C%22lastName%22%3A%22Elsnicova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jitka%20M.%22%2C%22lastName%22%3A%22Zurmanova%22%7D%5D%2C%22abstractNote%22%3A%22Cardioprotective%20effect%20of%20cold%20acclimation%20%285%5Cu00a0weeks%3B%209%5Cu00a0%5Cu00b0C%3B%20CA%29%20and%20the%20same%20effect%20persisting%202%5Cu00a0weeks%20after%20recovery%20from%20CA%20%28CAR%29%20are%20induced%20by%20different%20mechanisms.%20We%20showed%20that%20salvage%20%5Cu03b22-adrenoceptor%5C%2FGi%5C%2FAkt%20pathway%20is%20only%20involved%20in%20the%20mechanism%20of%20cardioprotection%20after%20CAR.%20Since%20the%20mechanism%20of%20CA-elicited%20cardioprotection%20is%20not%20known%2C%20we%20examined%20the%20role%20of%20JAK2%5C%2FSTAT3%20pathway.%20Male%20Wistar%20rats%20exposed%20to%20CA%20and%20controls%20%2824%5Cu00a0%5Cu00b0C%29%20were%20treated%20with%20the%20JAK2%20inhibitor%20AG490%20affecting%20downstream%20STAT3%20signaling%20in%20the%20heart%20%285%5Cu00a0mg%5C%2Fkg%5C%2Fday%29%20for%20three%20days%20prior%20to%20the%20end%20of%20experiment.%20AG490%20administration%20abolished%20CA-elicited%20reduction%20of%20infarct-size%20and%20significant%20improvement%20of%20MPT%20pore%20opening.%20IL-6%2C%20as%20the%20main%20STAT3%20upstream%20effector%2C%20was%20upregulated%20by%20CA%2C%20and%20AG490%20reversed%20its%20level.%20CA%20had%20no%20effect%20on%20IL-1%5Cu03b2%20and%20TNF-%5Cu03b1%2C%20but%20was%20upregulated%20by%20AG490%20in%20controls%2C%20and%20downregulated%20in%20CA-AG490%20group.%20CA%20also%20reduced%20pro-apoptotic%20p38-MAPK%2C%20which%20was%20abolished%20by%20AG490%20administration.%20Spatial%20expression%20analyses%20revealed%20CA-elicited%20translocation%20of%20total-STAT3%20from%20mitochondria%20to%20sarcolemma%20compartment%20which%20was%20eliminated%20by%20JAK2%5C%2FSTAT3%20inhibition.%20However%2C%20CA-induced%20loss%20of%20pSTAT3Y705%20from%20sarcolemma%20compartment%2C%20and%20loss%20of%20pSTAT3S727%20from%20nucleus.%20These%20results%20identify%20non-genomic%2C%20mitochondria-associated%20STAT3%20activity%20as%20a%20confirmed%20mechanism%20of%20CA-elicited%20cardioprotection.%22%2C%22date%22%3A%222026-03-05%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-026-40532-4%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-026-40532-4%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%222045-2322%22%2C%22language%22%3A%22en%22%2C%22collections%22%3A%5B%222FM3Z7J9%22%5D%2C%22dateModified%22%3A%222026-05-05T11%3A33%3A15Z%22%7D%7D%2C%7B%22key%22%3A%22SAXVG2AA%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Pinto%20et%20al.%22%2C%22parsedDate%22%3A%222026-03-01%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BPinto%2C%20A.%3B%20Amodio%2C%20L.%3B%20Kub%26%23x16F%3B%2C%20M.%3B%20Cueto%2C%20J.%3B%20Eli%26%23xE1%3B%26%23x161%3Bov%26%23xE1%3B%2C%20P.%3B%20Pizarro%2C%20P.%3B%20%26%23x10C%3Bejka%2C%20J.%3B%20Serrano%2C%20D.%20P.%20%26lt%3Bb%26gt%3BDechlorination%20and%20Upgrading%20of%20Waste%20Plastics%20Pyrolysis%20Oil%20over%20MWW%20and%20TUN%20Zeolites%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BCatalysis%20Today%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2026%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B463%26lt%3B%5C%2Fi%26gt%3B%2C%20115594.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.cattod.2025.115594%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.cattod.2025.115594%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Dechlorination%20and%20upgrading%20of%20waste%20plastics%20pyrolysis%20oil%20over%20MWW%20and%20TUN%20zeolites%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alberto%22%2C%22lastName%22%3A%22Pinto%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lidia%22%2C%22lastName%22%3A%22Amodio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22Kub%5Cu016f%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jennifer%22%2C%22lastName%22%3A%22Cueto%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pavla%22%2C%22lastName%22%3A%22Eli%5Cu00e1%5Cu0161ov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patricia%22%2C%22lastName%22%3A%22Pizarro%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ji%5Cu0159%5Cu00ed%22%2C%22lastName%22%3A%22%5Cu010cejka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%20P.%22%2C%22lastName%22%3A%22Serrano%22%7D%5D%2C%22abstractNote%22%3A%22Pyrolysis%20is%20a%20promising%20technique%20for%20converting%20waste%20plastics%20into%20oil%2C%20but%20the%20resulting%20liquid%20often%20contains%20halogens%2C%20particularly%20chlorine%2C%20which%20pose%20environmental%20and%20corrosion%20risks%20when%20used%20as%20fuel%20or%20refinery%20feedstock.%20These%20halogens%20primarily%20originate%20from%20materials%20such%20as%20PVC%20and%20other%20Cl-containing%20compounds%20present%20in%20the%20waste.%20This%20study%20evaluates%20the%20performance%20of%20several%20zeolites%20%28TNU-9%2C%20MCM-22%2C%20MCM-49%2C%20MCM-56%2C%20and%20MCM-36%29%20in%20the%20catalytic%20dehalogenation%20and%20upgrading%20of%20a%20real%20pyrolysis%20oil%20derived%20from%20waste%20plastics%20with%20a%20chlorine%20content%20of%20290%5Cu202fppm.%20Experiments%20were%20conducted%20using%20a%20fixed-bed%20catalytic%20reactor%20at%20450%5Cu202f%5Cu00b0C.%20All%20the%20tested%20catalysts%20improved%20the%20oil%20properties%2C%20decreasing%20its%20paraffin%20content%20and%20favouring%20the%20formation%20of%20aromatic%2C%20cyclic%20and%20olefinic%20hydrocarbons.%20This%20is%20positive%20regarding%20the%20possible%20use%20of%20this%20oil%20fraction%20in%20the%20formulation%20of%20transportation%20fuels%20or%20as%20a%20source%20of%20raw%20chemicals.%20The%20deepest%20modification%20was%20observed%20over%20MCM-36%20zeolite%2C%20achieving%20the%20highest%20cracking%20activity%20into%20light%20olefins%20%28mainly%20C3%20and%20C4%29.%20Its%20superior%20performance%20is%20attributed%20to%20its%20high%20external%20surface%20area%2C%20mesopore%20volume%2C%20and%20accessible%20Br%5Cu00f8nsted%20acid%20sites%2C%20resulting%20from%20its%20pillarized%20structure.%20Moreover%2C%20MCM-36%20was%20also%20the%20zeolite%20exhibiting%20the%20best%20oil%20dechlorination%20capability%2C%20which%20remained%20relatively%20stable%20along%20the%20time%20on%20stream%20in%20contrast%20to%20other%20zeolite%20catalysts.%20Analyses%20of%20the%20spent%20catalysts%20indicated%20that%20the%20deposited%20carbonaceous%20matter%20accumulates%20most%20of%20the%20Cl-containing%20species%20removed%20from%20the%20oil%3B%20hence%2C%20the%20overall%20oil%20upgrading%20process%20can%20be%20considered%20a%20combination%20of%20catalytic%20and%20adsorption%20effects.%20This%20material%20could%20be%20effectively%20regenerated%20by%20a%20sequential%20combination%20of%20Soxhlet%20extraction%20and%20calcination%20treatments.%22%2C%22date%22%3A%222026-03-01%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.cattod.2025.115594%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.sciencedirect.com%5C%2Fscience%5C%2Farticle%5C%2Fpii%5C%2FS0920586125004122%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220920-5861%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%222FM3Z7J9%22%5D%2C%22dateModified%22%3A%222026-04-20T06%3A36%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22862T8RFK%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Siahaan%20et%20al.%22%2C%22parsedDate%22%3A%222026-01-27%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BSiahaan%2C%20V.%3B%20Weissova%2C%20R.%3B%20Karhanova%2C%20A.%3B%20Lanska%2C%20E.%3B%20Ruiz-Estrada%2C%20M.%20J.%3B%20Pukajov%26%23xE1%3B%2C%20B.%3B%20Dost%26%23xE1%3Bl%2C%20V.%3B%20Henriot%2C%20V.%3B%20Janke%2C%20C.%3B%20Libusov%26%23xE1%3B%2C%20L.%3B%20Braun%2C%20M.%3B%20Balastik%2C%20M.%3B%20Lansky%2C%20Z.%20%26lt%3Bb%26gt%3BTau%20Phosphorylation%20Impedes%20Functionality%20of%20Protective%20Tau%20Envelopes%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BNat%20Chem%20Biol%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2026%26lt%3B%5C%2Fb%26gt%3B%2C%201%26%23x2013%3B11.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41589-025-02122-9%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41589-025-02122-9%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Tau%20phosphorylation%20impedes%20functionality%20of%20protective%20tau%20envelopes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Valerie%22%2C%22lastName%22%3A%22Siahaan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Romana%22%2C%22lastName%22%3A%22Weissova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adela%22%2C%22lastName%22%3A%22Karhanova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eva%22%2C%22lastName%22%3A%22Lanska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mar%5Cu00eda%20J.%22%2C%22lastName%22%3A%22Ruiz-Estrada%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Barbora%22%2C%22lastName%22%3A%22Pukajov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vojt%5Cu011bch%22%2C%22lastName%22%3A%22Dost%5Cu00e1l%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Veronique%22%2C%22lastName%22%3A%22Henriot%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carsten%22%2C%22lastName%22%3A%22Janke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lenka%22%2C%22lastName%22%3A%22Libusov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marcus%22%2C%22lastName%22%3A%22Braun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22Balastik%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zdenek%22%2C%22lastName%22%3A%22Lansky%22%7D%5D%2C%22abstractNote%22%3A%22Tau%20is%20an%20axonal%20microtubule-associated%20protein.%20Tau%20interaction%20with%20microtubules%20is%20regulated%20by%20phosphorylation.%20Hyperphosphorylation%20of%20tau%20is%20implicated%20in%20microtubule%20destabilization%20related%20to%20neurodegenerative%20disorders.%20However%2C%20how%20tau%20phosphorylation%20leads%20to%20microtubule%20destabilization%20is%20unknown.%20Recently%2C%20it%20was%20shown%20that%20tau%20molecules%20on%20microtubules%20cooperatively%20assemble%20into%20cohesive%20layers%20termed%20envelopes.%20Tau%20envelopes%20protect%20microtubules%20against%20degradation%20by%20microtubule-severing%20enzymes%2C%20suggesting%20a%20functional%20link%20between%20envelopes%20and%20microtubule%20stability.%20Here%20we%20show%20that%20tau%20phosphorylation%20has%20deleterious%20effects%20on%20the%20microtubule-protective%20function%20of%20tau%20envelopes.%20Using%20reconstitution%20and%20live-cell%20experiments%2C%20we%20found%20that%20tau%20phosphorylation%20destabilizes%20tau%20envelopes%20and%20decreases%20their%20integrity%2C%20leading%20to%20reduced%20microtubule%20protection%20against%20microtubule-severing%20enzymes.%20Our%20data%20suggest%20that%20a%20perturbation%20of%20microtubule%20homeostasis%20linked%20to%20tau%20hyperphosphorylation%20in%20neurodegeneration%20can%20be%20explained%20by%20the%20disassembly%20and%20impaired%20functionality%20of%20the%20tau%20envelopes.%22%2C%22date%22%3A%222026-01-27%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41589-025-02122-9%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41589-025-02122-9%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221552-4469%22%2C%22language%22%3A%22en%22%2C%22collections%22%3A%5B%222FM3Z7J9%22%5D%2C%22dateModified%22%3A%222026-02-06T14%3A22%3A08Z%22%7D%7D%2C%7B%22key%22%3A%22R4SV68QC%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22B%5Cu0159ezina%20et%20al.%22%2C%22parsedDate%22%3A%222026-01-02%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BB%26%23x159%3Bezina%2C%20J.%3B%20Brabec%2C%20T.%3B%20Macha%26%23x10D%3B%2C%20D.%3B%20Vobo%26%23x159%3Bil%2C%20M.%3B%20Ballek%2C%20O.%3B%20Pa%26%23x10D%3Bes%2C%20J.%3B%20S%26%23xFD%3Bkora%2C%20V.%3B%20Jan%26%23x10D%3Bovi%26%23x10D%3Bov%26%23xE1%3B%2C%20K.%3B%20Valter%2C%20E.%3B%20Kov%26%23xE1%3B%26%23x10D%3Bov%26%23xE1%3B%2C%20K.%3B%20Manning%2C%20J.%3B%20Tahtahov%26%23xE1%3B%2C%20V.%3B%20%26%23x10C%3Bepkov%26%23xE1%3B%2C%20A.%3B%20Dobe%26%23x161%3Bov%26%23xE1%3B%2C%20M.%3B%20Dobe%26%23x161%3B%2C%20J.%3B%20Kubov%26%23x10D%3Biak%2C%20J.%3B%20Kol%26%23xE1%3B%26%23x159%3B%2C%20M.%3B%20Ka%26%23x161%3Bp%26%23xE1%3Brek%2C%20P.%3B%20Sedlacek%2C%20R.%3B%20%26%23x160%3Btep%26%23xE1%3Bnek%2C%20O.%3B%20%26%23x10C%3Bern%26%23xFD%3B%2C%20J.%3B%20Tsukita%2C%20S.%3B%20Malissen%2C%20B.%3B%20Anderson%2C%20G.%3B%20Filipp%2C%20D.%20%26lt%3Bb%26gt%3BClaudin%201%26%23x2013%3BMediated%20Positioning%20of%20DC1%20to%20mTECs%20Is%20Essential%20for%20Maintenance%20of%20Central%20Tolerance%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BJ%20Exp%20Med%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2026%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B223%26lt%3B%5C%2Fi%26gt%3B%20%283%29%2C%20e20250970.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-ItemURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1084%5C%2Fjem.20250970%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1084%5C%2Fjem.20250970%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Claudin%201%5Cu2013mediated%20positioning%20of%20DC1%20to%20mTECs%20is%20essential%20for%20maintenance%20of%20central%20tolerance%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ji%5Cu0159%5Cu00ed%22%2C%22lastName%22%3A%22B%5Cu0159ezina%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tom%5Cu00e1%5Cu0161%22%2C%22lastName%22%3A%22Brabec%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Macha%5Cu010d%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matou%5Cu0161%22%2C%22lastName%22%3A%22Vobo%5Cu0159il%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ond%5Cu0159ej%22%2C%22lastName%22%3A%22Ballek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jan%22%2C%22lastName%22%3A%22Pa%5Cu010des%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vojt%5Cu011bch%22%2C%22lastName%22%3A%22S%5Cu00fdkora%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Krist%5Cu00edna%22%2C%22lastName%22%3A%22Jan%5Cu010dovi%5Cu010dov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Evgeny%22%2C%22lastName%22%3A%22Valter%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katar%5Cu00edna%22%2C%22lastName%22%3A%22Kov%5Cu00e1%5Cu010dov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jasper%22%2C%22lastName%22%3A%22Manning%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Valerie%22%2C%22lastName%22%3A%22Tahtahov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ad%5Cu00e9la%22%2C%22lastName%22%3A%22%5Cu010cepkov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martina%22%2C%22lastName%22%3A%22Dobe%5Cu0161ov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jan%22%2C%22lastName%22%3A%22Dobe%5Cu0161%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jan%22%2C%22lastName%22%3A%22Kubov%5Cu010diak%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michal%22%2C%22lastName%22%3A%22Kol%5Cu00e1%5Cu0159%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petr%22%2C%22lastName%22%3A%22Ka%5Cu0161p%5Cu00e1rek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Radislav%22%2C%22lastName%22%3A%22Sedlacek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ond%5Cu0159ej%22%2C%22lastName%22%3A%22%5Cu0160tep%5Cu00e1nek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jan%22%2C%22lastName%22%3A%22%5Cu010cern%5Cu00fd%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sachiko%22%2C%22lastName%22%3A%22Tsukita%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bernard%22%2C%22lastName%22%3A%22Malissen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Graham%22%2C%22lastName%22%3A%22Anderson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dominik%22%2C%22lastName%22%3A%22Filipp%22%7D%5D%2C%22abstractNote%22%3A%22Central%20tolerance%2C%20which%20relies%20on%20the%20presentation%20of%20self-antigens%20by%20mTECs%20and%20DCs%2C%20prevents%20autoimmunity%20by%20eliminating%20self-reactive%20T%20cells.%20While%20mTECs%20produce%20self-antigens%20autonomously%2C%20DCs%20acquire%20them%20from%20mTECs%20via%20cooperative%20antigen%20transfer%20%28CAT%29.%20We%20previously%20showed%20that%20mTEC%20and%20DC%20subsets%20exhibit%20preferential%20pairing%20in%20CAT%2C%20providing%20a%20rationale%20for%20the%20existence%20of%20molecular%20determinants%20underpinning%20this%20pairing%20and%20its%20outcome.%20Here%2C%20we%20compared%20the%20transcriptomes%20of%20CAT-experienced%20and%20CAT-inexperienced%20DCs%20and%20identified%20Claudin%201%20as%20a%20molecule%20involved%20in%20CAT%20and%20type%201%20DC%20%28DC1%29%20maturation.%20DC1-specific%20ablation%20of%20Claudin%201%20resulted%20in%20decreased%20CAT%20to%20late%20mature%20DC1s%20and%20dramatically%20diminished%20DC1%20maturation.%20These%20phenotypes%20correlated%20with%20the%20displacement%20of%20DC1s%20from%20mTECs%20and%20their%20decreased%20expression%20of%20MHCII%20pathway%20genes.%20This%20translated%20into%20impaired%20Treg%20selection%20and%20clonal%20deletion%2C%20ultimately%20manifesting%20in%20symptoms%20of%20multiorgan%20autoimmunity%20and%20shortened%20lifespan.%20Collectively%2C%20our%20results%20identify%20thymic%20DC1-derived%20Claudin%201%20as%20a%20regulator%20of%20immune%20tolerance.%22%2C%22date%22%3A%222026-01-02%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1084%5C%2Fjem.20250970%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1084%5C%2Fjem.20250970%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220022-1007%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%222FM3Z7J9%22%5D%2C%22dateModified%22%3A%222026-01-03T20%3A46%3A25Z%22%7D%7D%2C%7B%22key%22%3A%22U56WV2GN%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Garc%5Cu00eda-Gonz%5Cu00e1lez%20et%20al.%22%2C%22parsedDate%22%3A%222026%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BGarc%26%23xED%3Ba-Gonz%26%23xE1%3Blez%2C%20J.%3B%20Havelkov%26%23xE1%3B%2C%20L.%3B%20Bellinvia%2C%20E.%3B%20Corrales%2C%20I.%3B%20Pejchar%2C%20P.%3B%20Potock%26%23xFD%3B%2C%20M.%3B%20Schwarzerov%26%23xE1%3B%2C%20K.%20%26lt%3Bb%26gt%3BIndependent%20Roles%20of%20Arp2%5C%2F3%20Complex%20and%20RIC4%20Protein%20in%20the%20Control%20of%20Epidermal%20Cell%20Shape%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BPlanta%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2026%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B263%26lt%3B%5C%2Fi%26gt%3B%20%284%29%2C%20110.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs00425-026-04976-2%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs00425-026-04976-2%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Independent%20roles%20of%20Arp2%5C%2F3%20complex%20and%20RIC4%20protein%20in%20the%20control%20of%20epidermal%20cell%20shape%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Judith%22%2C%22lastName%22%3A%22Garc%5Cu00eda-Gonz%5Cu00e1lez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lenka%22%2C%22lastName%22%3A%22Havelkov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Erica%22%2C%22lastName%22%3A%22Bellinvia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Israel%22%2C%22lastName%22%3A%22Corrales%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P%5Cu0159emysl%22%2C%22lastName%22%3A%22Pejchar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22Potock%5Cu00fd%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kate%5Cu0159ina%22%2C%22lastName%22%3A%22Schwarzerov%5Cu00e1%22%7D%5D%2C%22abstractNote%22%3A%22Main%20conclusion%5CnOur%20results%20show%20that%20RIC4%20and%20the%20Arp2%5C%2F3%20complex%20function%20largely%20independently%20in%20the%20control%20of%20pavement%20cell%20shape.%20Genetic%20analyses%20indicate%20that%20they%20act%20in%20parallel%20rather%20than%20in%20one%20signaling%20cascade.%20We%20further%20demonstrate%20that%20RIC4%20functions%20as%20a%20negative%20regulator%20of%20lobe%20formation%2C%20as%20its%20loss%20increases%20cell%20shape%20complexity%20whereas%20its%20overexpression%20increases%20cell%20circularity.%20RIC4-induced%20cell%20shape%20changes%20occur%20even%20in%20the%20absence%20of%20a%20functional%20Arp2%5C%2F3%20complex%2C%20excluding%20Arp2%5C%2F3%20as%20a%20downstream%20effector%20of%20RIC4%20or%20ROP%20signaling.%20Finally%2C%2022%20no%20correlation%20between%20cortical%20actin%20dynamics%20and%20cell%20shape%20phenotypes%20was%20detected%2C%20which%20suggests%20that%20global%20actin%20dynamics%20alone%20cannot%20explain%20pavement%20cell%20morphogenesis.%5Cn%5CnAbstract%5CnThe%20aim%20of%20this%20study%20was%20to%20determine%20whether%20a%20Cdc42%5C%2FRac%20interactive%20binding%20%28CRIB%29%20domain-containing%20protein%204%20%28RIC4%29%20that%20functions%20as%20an%20effector%20of%20ROP%20GTPases%2C%20and%20the%20Arp2%5C%2F3%20complex%2C%20an%20actin%20nucleator%2C%20functionally%20cooperate%20in%20controlling%20the%20shape%20of%20Arabidopsis%20cotyledon%20epidermal%20cells.%20The%20combination%20of%20knock-out%20mutants%20demonstrated%20that%20loss%20of%20RIC4%20and%20loss%20of%20the%20Arp2%5C%2F3%20complex%20results%20in%20completely%20opposite%20epidermal%20cell%20shape%20phenotypes.%20The%20double%20knock-out%20%28KO%29%20mutation%20phenotype%20is%20similar%20to%20the%20Arp2%5C%2F3%20mutation%2C%20and%20the%20effect%20of%20RIC4%20loss%20is%20completely%20eliminated.%20Analysis%20of%20overexpression%20revealed%20that%20excess%20RIC4%20significantly%20suppresses%20the%20formation%20of%20pavement%20cell%20lobes.%20However%2C%20RIC4%20does%20not%20require%20an%20active%20Arp2%5C%2F3%20complex%20for%20this%20effect.%20Our%20data%20further%20show%20that%20overexpression%20of%20RIC4%20has%20a%20specific%20actin%20stabilization%20effect%20in%20cotyledon%20epidermal%20cells.%20Interestingly%2C%20while%20RIC4%20overexpression%20induced%20actin%20stabilization%20and%20reduced%20cell-shape%20complexity%2C%20the%20loss%20of%20Arp2%5C%2F3%20with%20a%20similar%20cell-shape%20phenotype%20did%20not%20show%20reduced%20actin%20dynamics.%20In%20conclusion%2C%20RIC4%20and%20the%20Arp2%5C%2F3%20complex%20do%20not%20share%20the%20same%20signaling%20pathway%20in%20the%20control%20of%20cotyledon%20epidermal%20cell%20shape.%5Cn%5CnSupplementary%20Information%5CnThe%20online%20version%20contains%20supplementary%20material%20available%20at%2010.1007%5C%2Fs00425-026-04976-2.%22%2C%22date%22%3A%222026%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1007%5C%2Fs00425-026-04976-2%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fpmc.ncbi.nlm.nih.gov%5C%2Farticles%5C%2FPMC12995933%5C%2F%22%2C%22PMID%22%3A%2241845129%22%2C%22PMCID%22%3A%22PMC12995933%22%2C%22ISSN%22%3A%220032-0935%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%222FM3Z7J9%22%5D%2C%22dateModified%22%3A%222026-03-31T13%3A19%3A24Z%22%7D%7D%5D%7D
1.
Schätz, M.; Sugawara, K. The Reproducibility Gap in Graph Neural Network Workflows for Cell Dynamics: A Checklist-Driven Case Study. Journal of Microscopy 2026, n/a (n/a). https://doi.org/10.1111/jmi.70139.
1.
Corbat, A. A.; Walther, C. G.; de la Ballina, L. R.; Condon, N. D.; Felder, A. A.; Schätz, M.; Schmerl, B.; Sugawara, K.; Prats, C.; Klemm, A.; Miura, K.; Sampaio, P.; Tischer, C.; Levet, F.; D’Antuono, R.; Cimini, B. A.; Haase, R. GloBIAS: Strengthening the Foundations of Bioimage Analysis. Nat Methods 2026, 1–2. https://doi.org/10.1038/s41592-026-03060-7.
1.
Kasik, P.; Galatik, F.; Matous, P.; Paral, P.; Vasek, D.; Horníkova, D.; Elsnicova, B.; Zurmanova, J. M. Causal Relevance of the JAK/STAT Pathway for Cardioprotection via Cold Acclimation. Sci Rep 2026, 16 (1), 12124. https://doi.org/10.1038/s41598-026-40532-4.
1.
Pinto, A.; Amodio, L.; Kubů, M.; Cueto, J.; Eliášová, P.; Pizarro, P.; Čejka, J.; Serrano, D. P. Dechlorination and Upgrading of Waste Plastics Pyrolysis Oil over MWW and TUN Zeolites. Catalysis Today 2026, 463, 115594. https://doi.org/10.1016/j.cattod.2025.115594.
1.
Siahaan, V.; Weissova, R.; Karhanova, A.; Lanska, E.; Ruiz-Estrada, M. J.; Pukajová, B.; Dostál, V.; Henriot, V.; Janke, C.; Libusová, L.; Braun, M.; Balastik, M.; Lansky, Z. Tau Phosphorylation Impedes Functionality of Protective Tau Envelopes. Nat Chem Biol 2026, 1–11. https://doi.org/10.1038/s41589-025-02122-9.
1.
Březina, J.; Brabec, T.; Machač, D.; Vobořil, M.; Ballek, O.; Pačes, J.; Sýkora, V.; Jančovičová, K.; Valter, E.; Kováčová, K.; Manning, J.; Tahtahová, V.; Čepková, A.; Dobešová, M.; Dobeš, J.; Kubovčiak, J.; Kolář, M.; Kašpárek, P.; Sedlacek, R.; Štepánek, O.; Černý, J.; Tsukita, S.; Malissen, B.; Anderson, G.; Filipp, D. Claudin 1–Mediated Positioning of DC1 to mTECs Is Essential for Maintenance of Central Tolerance. J Exp Med 2026, 223 (3), e20250970. https://doi.org/10.1084/jem.20250970.
1.
García-González, J.; Havelková, L.; Bellinvia, E.; Corrales, I.; Pejchar, P.; Potocký, M.; Schwarzerová, K. Independent Roles of Arp2/3 Complex and RIC4 Protein in the Control of Epidermal Cell Shape. Planta 2026, 263 (4), 110. https://doi.org/10.1007/s00425-026-04976-2.
2025
5891878
JRFQ2MKI
1
https://raw.githubusercontent.com/Schebique/vmcf-konfmi/refs/heads/main/vmcf-web-style.csl
50
date
desc
4983
https://web.natur.cuni.cz/sekce-bi/VMCF/wp-content/plugins/zotpress/
statussuccessupdateneededfalseinstancefalsemetarequest_last0request_next0used_cachetruedatakeyZ3ZUCGHJlibraryid5891878metacreatorSummaryKuu010derovu00e1etal.parsedDate2025-12-01numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKux10DerovxE1J.ZdrhaA.RozbeskxFDD.ShindeA.P.NebesxE1x159ovxE1J.NarayanasamyR.K.SmutnxE1T.HrdxFDI.TachezyJ.ltbgtCharacterizationofthesTimMIAPathwayinMetamonadaRevealsDifferentEvolutionaryAdaptationstoAnaerobiosisltbgt.ltigtCurrentBiologyltigtltbgt2025ltbgtltigt35ltigt235734-5749.e6.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.cub.2025.10.027039gthttpsdoi.org10.1016j.cub.2025.10.027ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleCharacterizationofthesTimMIApathwayinMetamonadarevealsdifferentevolutionaryadaptationstoanaerobiosiscreatorscreatorTypeauthorfirstNameJitkalastNameKuu010derovu00e1creatorTypeauthorfirstNameAloislastNameZdrhacreatorTypeauthorfirstNameDaniellastNameRozbesku00fdcreatorTypeauthorfirstNameAbhishekPrakashlastNameShindecreatorTypeauthorfirstNameJanalastNameNebesu00e1u0159ovu00e1creatorTypeauthorfirstNameRaviKumarlastNameNarayanasamycreatorTypeauthorfirstNameTamaralastNameSmutnu00e1creatorTypeauthorfirstNameIvanlastNameHrdu00fdcreatorTypeauthorfirstNameJanlastNameTachezyabstractNoteAdaptationofeukaryoticcellstooxygen-poorenvironmentshasdrivenmetabolicchangesinmitochondrianotablyshiftingfromoxygen-dependenttoanaerobicenergymetabolism.HoweverhowthemitochondrialproteinimportmachineryadaptsinanaerobesremainspoorlyunderstoodalthoughoxygeniscrucialforthisprocessparticularlyforoxidativefoldingofsmallTimsTimchaperones.sTimheterohexamericcomplexesguideimportedproteinswithinthemitochondrialintermembranespaceIMS.TheirfunctiondependsonconservedtwincysteinesoxidizedbythemitochondrialimportandassemblyMIApathwaytostabilizetheirstructureviadisulfidebridges.ThefoldingrequiresmolecularoxygenorcytochromecaselectronacceptorslinkingsTimfoldingtorespiration.ThisstudyelucidateshowthesTimMIApathwayisreshapedinanaerobictypesofmitochondriasuchashydrogenosomes.ThroughstructuralandhomologyanalysesacrossanaerobiceukaryotesthreemodificationsofthesTimMIAsystemwereidentified1adisulfiderelay-independentsystemwithsTimslackingtwincysteinessTimu2212cys2absenceofsTimMIAcomponentsand3aconventionalsTimMIAsystemlinkedtofumaratereduction.ThesTimu2212cyssystemfoundinMetamonadawasstudiedinTrichomonasvaginalishydrogenosomes.StructuralmodelinginvitroandinsituanalysesrevealedthatdespitelackingcanonicalcysteinessTimu2212cysproteinsmaintainthehelix-loop-helixarchitecturewiththecentralloopinvolvedintargetingtotheIMSandassembleintocomplexesstabilizedbyelectrostaticinteractions.Single-particleanalysisconfirmedtheir6-foldsymmetrysimilartoconventionalsTimheterohexamers.ThesefindingsprovideinsightsintotheevolutionaryshapingofsTimMIApathwaysinanoxicenvironmentscontributingtoourunderstandingofmitochondrialbiogenesisacrossdiverseeukaryotes.date2025-12-01sectionpartNumberpartTitleDOI10.1016j.cub.2025.10.027citationKeyurlhttpswww.sciencedirect.comsciencearticlepiiS0960982225013351PMIDPMCIDISSN0960-9822languagecollectionsJRFQ2MKIdateModified2025-12-29T132130ZkeyH6N6RHRNlibraryid5891878metacreatorSummaryBourlandetal.parsedDate2025-12-01numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtBourlandW.Pomahax10DO.MxE9ndez-SxE1nchezD.BeinartR.A.BernhardJ.M.x10Cepix10DkaI.RotterovxE1J.ltbgtTheEndofaWindingPathTheAnaerobicCiliateltigtSpirorhynchusltigtIsaMemberoftheClassMuranotrichealtbgt.ltigtProtistltigtltbgt2025ltbgtltigt179ltigt126129.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.protis.2025.126129039gthttpsdoi.org10.1016j.protis.2025.126129ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTheendofawindingpathTheanaerobicciliateiSpirorhynchusiisamemberoftheclassMuranotricheacreatorscreatorTypeauthorfirstNameWilliamlastNameBourlandcreatorTypeauthorfirstNameOndu0159ejlastNamePomahau010dcreatorTypeauthorfirstNameDaniellastNameMu00e9ndez-Su00e1nchezcreatorTypeauthorfirstNameRoxanneA.lastNameBeinartcreatorTypeauthorfirstNameJoanM.lastNameBernhardcreatorTypeauthorfirstNameIvanlastNameu010cepiu010dkacreatorTypeauthorfirstNameJohanalastNameRotterovu00e1abstractNoteCiliatedprotistsCiliophoraareimportantmembersoffreshwaterbrackishmarineandhypersalinebenthicmicrobialcommunities.AspartofourbroaderstudiesofanaerobicprotistsweencounteredaciliateinhypoxicsedimentsamplesfromthreegeographicallydistantsalinehabitatsandidentifieditasSpirorhynchusverrucosusCunha1915.ThishighlyunusualciliatehasacomplicatednomenclaturalandtaxonomichistoryandhasbeenassignedtothearmophoreanfamilyMetopidae.Thedistinctivecellshapeanduniquearrangementofectosymbiontsallowsidentificationofthisspeciesbyinvivoobservation.Twopopulationsofanotherciliatefoundindeep-seasedimentswererecognizedasamorphologicallyandgeneticallydistinctbutnotyetformallydescribedSpirorhynchusspecies.MuranotricheaRotterovu00e1etal.2020isarecentlyestablishedclassofobligatelyanaerobicmarineciliatesthatbearprokaryoticectosymbiontsandincludesonefamilywithtwogeneraandthreespecies.Phylogeneticanalysesbasedonthefirst18SrRNAgenesequencesfromthegenusplaceSpirorhynchusinClassMuranotrichea.Insingle-genetreesSpirorhynchusismonophyleticbranchingastheclosestrelativeofamarineenvironmentalsequenceandthemuranotricheangenusThigmothrixwithwhichSpirorhynchusalsosharessomemorphologicsimilarities.HerewealsoprovidethefirstprotargolimpregnationsandscanningelectronmicroscopyimagesfromSpirorhynchus.date2025-12-01sectionpartNumberpartTitleDOI10.1016j.protis.2025.126129citationKeyurlhttpswww.sciencedirect.comsciencearticlepiiS1434461025000458PMIDPMCIDISSN1434-4610languagecollectionsJRFQ2MKIdateModified2025-10-25T082255ZkeyWM9VSTP2libraryid5891878metacreatorSummaryu0160kaloudetal.parsedDate2025-12numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtx160kaloudP.Tux10DkovxE1K.x10CablovxE1R.JadrnxE1I.x10CernajovxE1I.ltbgtHighx2010frequencySamplingUnveilsBioticandAbioticDriversofRapidPhytoplanktonMorphologicalChangesltbgt.ltigtNewPhytolltigtltbgt2025ltbgtltigt248ltigt52528x20132541.ltaclass039zp-DOIURL039href039httpsdoi.org10.1111nph.70534039gthttpsdoi.org10.1111nph.70534ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleHighu2010frequencysamplingunveilsbioticandabioticdriversofrapidphytoplanktonmorphologicalchangescreatorscreatorTypeauthorfirstNamePavellastNameu0160kaloudcreatorTypeauthorfirstNameKateu0159inalastNameTuu010dkovu00e1creatorTypeauthorfirstNameRadkalastNameu010cablovu00e1creatorTypeauthorfirstNameIvalastNameJadrnu00e1creatorTypeauthorfirstNameIvanalastNameu010cernajovu00e1abstractNotePhytoplanktonasprimaryproducersplayakeyroleinaquaticecosystems.Theircommunityturnoverisshapedbymorphologicaltraitsthatenableadaptationtodiverseabioticandbioticfactors.Yetthetemporalscaleofthesedynamicsremainspoorlyunderstoodduetolimitedhighu2010frequencysamplingstudies.EmployingDNAmetabarcodingweassessedthecommunitycompositionofthephytoplanktonlineageSynuralesChrysophyceaeat3u2010dintervalsduring70u2009datashallowpeatboglakeintheCzechRepublic.Theselectedgrouppossessesavarietyofspeciesu2010specifickeymorphologicaltraitssuchascellsizecolonialityandbristleformation.Usingacustomreferencedatabaseofculturedspeciesweassigned99.93ofeDNAreadsto74speciesu2010levellineageswithknownmorphologicaltraits.Communitychangesincolonialspecieswereinfluencedbyabioticdriverssuchassilicaconcentrationandwindspeed.BycontrastshiftsinunicellularspeciescommunitiesweremainlydrivenbyCladocerapredatorsinfluencingtheoccurrenceofbristleu2010bearingspecies.Changesinspeciescompositionandmorphologicaltraitsoccurredwithindaysmirroringenvironmentalvariability.Achievingsuchfineu2010scaleresolutionespeciallyforsmallorraretaxawouldbeextremelydifficultusingmicroscopyalone.eDNAenabledhighu2010resolutioncommunityprofilingandabundanceestimationdemonstratingitskeyroleandtheimportanceofcomprehensivereferencedatabases.date2025-12sectionpartNumberpartTitleDOI10.1111nph.70534citationKeyurlhttpspmc.ncbi.nlm.nih.govarticlesPMC12589706PMID40908506PMCIDPMC12589706ISSN0028-646XlanguagecollectionsJRFQ2MKIdateModified2026-04-20T064446Zkey68XNWBPAlibraryid5891878metacreatorSummaryHu00e1u010deketal.parsedDate2025-11-24numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHxE1x10DekJ.VlkovxE1A.KrsekD.Kolax159xEDkL.SpxE1lenkovxE1A.SychraT.Tesax159ovxE1T.GayibovE.VxE1clavxEDkovxE1R.ZiegJ.BielaszewskaM.ltbgtEnterohemorrhagicEscherichiaColiO157OuterMembraneVesiclesAdministeredbyOralGavageCauseRenalTubularInjuryandAcuteKidneyFailureinMiceltbgt.ltigtFront.Cell.Infect.Microbiol.ltigtltbgt2025ltbgtltigt15ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.3389fcimb.2025.1704731039gthttpsdoi.org10.3389fcimb.2025.1704731ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleEnterohemorrhagicEscherichiacoliO157outermembranevesiclesadministeredbyoralgavagecauserenaltubularinjuryandacutekidneyfailureinmicecreatorscreatorTypeauthorfirstNameJaromu00edrlastNameHu00e1u010dekcreatorTypeauthorfirstNameAlenalastNameVlkovu00e1creatorTypeauthorfirstNameDaniellastNameKrsekcreatorTypeauthorfirstNameLuku00e1u0161lastNameKolau0159u00edkcreatorTypeauthorfirstNameAlu017ebu011btalastNameSpu00e1lenkovu00e1creatorTypeauthorfirstNameTomu00e1u0161lastNameSychracreatorTypeauthorfirstNameTerezalastNameTesau0159ovu00e1creatorTypeauthorfirstNameEminlastNameGayibovcreatorTypeauthorfirstNameRadkalastNameVu00e1clavu00edkovu00e1creatorTypeauthorfirstNameJakublastNameZiegcreatorTypeauthorfirstNameMartinalastNameBielaszewskaabstractNoteBackgroundOutermembranevesiclesOMVssecretedbyenterohemorrhagicEscherichiacoliEHECO157containShigatoxin2Stx2themajorvirulencefactorinvolvedinthepathogenesisofEHEC-associatedhemolyticuremicsyndromeEHEC-HUS.HoweveritremainsunclearwhetherEHECOMVsproducedinthehumanintestineduringinfectionplayaroleinEHEC-HUSdevelopment.UsingamousemodelweinvestigatedwhetherEHECO157OMVsadministeredbyoralgavagetranslocatefromthegastrointestinaltracttothebloodstreamenterthekidneysandinducesignsofEHEC-HUS.BecausemiceunlikehumansexpresstheStx2receptorGb3ontherenaltubularepitheliumbutnotontheglomerularendotheliumwefocusedontheabilityofEHECO157OMVstocausetubulardamagewhichrepresentsamechanismthatalongsideglomerularthromboticmicroangiopathyTMAcontributestoacutekidneyfailureinEHEC-HUS.MethodsTheseraandkidneysofBALBcmiceorallyadministeredEHECO157OMVswereexaminedforOMVsbyimmunoelectronandconfocalimmunofluorescencemicroscopy.Histopathologicalevaluationofthekidneyswasperformedbylightandelectronmicroscopyandbloodanalyseswereconductedusingstandardmethods.ThecytotoxicityofEHECO157OMVstowardhumanrenalglomerularendothelialcellsHRGECsandtubularepithelialcellsHK-2wasdeterminedbyCellDeathELISA.InadditionserafrompatientswithEHECO157-associatedHUSwereexaminedforO157OMVsbyimmunoelectronmicroscopy.ResultsEHECO157OMVsweredetectedintheseraandkidneysofmiceorallyadministered100u2013400u00b5gofOMVs.Themiceexhibitedrenaltubularepithelialdamageandhadsignificantlyincreasedserumcreatinineandbloodureanitrogenlevelsindicatingacutekidneyfailure.EHECO157OMVsinducedapoptosisinHRGECsandHK-2cellstheprimarytargetsinEHEC-HUS.MoreoverEHECO157OMVswerefoundintheseraofpatientswithEHECO157-associatedHUS.ConclusionOrallyadministeredEHECO157OMVstranslocatedfromthegastrointestinaltracttothekidneyswheretheycausedtubularepithelialinjuryfollowedbyacutekidneyfailure.CombinedwiththeircytotoxicitytowardHRGECsandHK-2cellsanddetectioninpatientserathesefindingsindicatethatEHECO157OMVscontributetothepathogenesisofEHEC-HUS.date2025-11-24sectionpartNumberpartTitleDOI10.3389fcimb.2025.1704731citationKeyurlhttpswww.frontiersin.orgjournalscellular-and-infection-microbiologyarticles10.3389fcimb.2025.1704731fullPMIDPMCIDISSN2235-2988languageEnglishcollectionsJRFQ2MKIdateModified2025-11-27T112729ZkeyKLH55NH4libraryid5891878metacreatorSummaryu0160kaloudetal.parsedDate2025-10-16numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtx160kaloudP.x160kaloudovxE1M.KnotekP.JadrnxE1I.PusztaiM.ltbgtMallomonasIntermediaACaseStudyofSpeciationandEvolutionaryDynamicsofProtistsltbgt.ltigtFottealtigtltbgt2025ltbgtltigt25ltigt2169x2013182.ltaclass039zp-ItemURL039href039httpsdoi.org10.5507fot.2025.008039gthttpsdoi.org10.5507fot.2025.008ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMallomonasintermediaAcasestudyofspeciationandevolutionarydynamicsofprotistscreatorscreatorTypeauthorfirstNamePavellastNameu0160kaloudcreatorTypeauthorfirstNameMagdalastNameu0160kaloudovu00e1creatorTypeauthorfirstNamePetrlastNameKnotekcreatorTypeauthorfirstNameIvalastNameJadrnu00e1creatorTypeauthorfirstNameMartinlastNamePusztaiabstractNotePavelu0160kaloudMagdau0160kaloudovu00e1PetrKnotekIvaJadrnu00e1MartinPusztaidate20251016sectionpartNumberpartTitleDOI10.5507fot.2025.008citationKeyurlhttpsdoi.org10.5507fot.2025.008PMIDPMCIDISSN1802543918054927languageencollectionsJRFQ2MKIdateModified2025-10-30T083738ZkeyR6TZJNHZlibraryid5891878metacreatorSummaryCorbatetal.parsedDate2025-07-08numChildren3bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtCorbatA.A.WaltherC.G.BallinaL.R.delaCondonN.D.FelderA.A.SchxE4tzM.SchmerlB.SugawaraK.PratsC.KlemmA.LevetF.MiuraK.SampaioP.TischerC.Dx2019AntuonoR.CiminiB.A.HaaseR.ltbgtGloBIASStrengtheningtheFoundationsofBioImageAnalysisltbgt.arXivJuly82025.ltaclass039zp-DOIURL039href039httpsdoi.org10.48550arXiv.2507.06407039gthttpsdoi.org10.48550arXiv.2507.06407ltagt.ltdivgtnltdivgtnltdivgtdataitemTypepreprinttitleGloBIASstrengtheningthefoundationsofBioImageAnalysiscreatorscreatorTypeauthorfirstNameA.A.lastNameCorbatcreatorTypeauthorfirstNameC.G.lastNameWalthercreatorTypeauthorfirstNameL.R.delalastNameBallinacreatorTypeauthorfirstNameN.D.lastNameCondoncreatorTypeauthorfirstNameA.A.lastNameFeldercreatorTypeauthorfirstNameM.lastNameSchu00e4tzcreatorTypeauthorfirstNameB.lastNameSchmerlcreatorTypeauthorfirstNameK.lastNameSugawaracreatorTypeauthorfirstNameC.lastNamePratscreatorTypeauthorfirstNameA.lastNameKlemmcreatorTypeauthorfirstNameF.lastNameLevetcreatorTypeauthorfirstNameK.lastNameMiuracreatorTypeauthorfirstNameP.lastNameSampaiocreatorTypeauthorfirstNameC.lastNameTischercreatorTypeauthorfirstNameR.lastNameDAntuonocreatorTypeauthorfirstNameB.A.lastNameCiminicreatorTypeauthorfirstNameR.lastNameHaaseabstractNoteThereisaglobalneedforBioImageAnalysisBIAasadvancesinlifesciencesincreasinglyrelyoncutting-edgeimagingsystemsthathavedramaticallyexpandedthecomplexityanddimensionalityofbiologicalimages.Turningthesedataintoscientificdiscoveriesrequirespeoplewitheffectivedatamanagementskillsandknowledgeofstate-of-the-artimageprocessinganddataanalysisinotherwordsBioImageAnalysts.TheGlobalBioImageAnalysts039SocietyGloBIASaimstoenhancetheprofileofBioImageAnalystsasakeyroleinscienceandresearch.ItsvisionencompassesfosteringaglobalnetworkdemocratisingaccesstoBIAbyprovidingeducationalresourcestailoredtovariousproficiencylevelsanddisciplineswhilealsoestablishingguidelinesforBIAcourses.BycollaborativelyshapingtheeducationofBioImageAnalystsGloBIASaimstounlockthefullpotentialofBIAinadvancinglifescienceresearchandtoconsolidateBIAasacareerpath.TobetterunderstandtheneedsandgeographicalrepresentationoftheBIAcommunityaworldwidesurveywasconductedand291responseswerecollectedacrosspeoplefromallcareerstagesandcontinents.ThisworkdiscusseshowGloBIASaimstoaddresscommunity-identifiedshortcomingsinworkenvironmentfundingandscientificactivities.ThesurveyunderscoresastronginterestfromtheBIAcommunityinactivitiesproposedbyGloBIASandtheirinteresttoactivelycontribute.With72ofrespondentswillingtopayformembershipthecommunity039senthusiasmforbothonlineandin-personeventsissettodrivethegrowthandsustainabilityofGloBIAS.genrerepositoryarXivarchiveIDarXiv2507.06407date2025-07-08DOI10.48550arXiv.2507.06407citationKeyurlhttparxiv.orgabs2507.06407languagecollectionsJRFQ2MKIdateModified2025-07-17T084852ZkeyWPDXHKMRlibraryid5891878metacreatorSummaryHiu0159manetal.parsedDate2025-07-01numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHix159manM.KaramanI.x160tundlovxE1J.JustP.x160x165xE1hlavskxFDF.ltbgtEvolutionaryDynamicsoftheChromosomalChangesintheGenusCyphophthalmusArachnidaOpilionesontheBalkanPeninsulaltbgt.ltigtZoolJLinnSocltigtltbgt2025ltbgtltigt204ltigt3zlaf068.ltaclass039zp-ItemURL039href039httpsdoi.org10.1093zoolinneanzlaf068039gthttpsdoi.org10.1093zoolinneanzlaf068ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleEvolutionarydynamicsofthechromosomalchangesinthegenusCyphophthalmusArachnidaOpilionesontheBalkanPeninsulacreatorscreatorTypeauthorfirstNameMatyu00e1u0161lastNameHiu0159mancreatorTypeauthorfirstNameIvolastNameKaramancreatorTypeauthorfirstNameJanalastNameu0160tundlovu00e1creatorTypeauthorfirstNamePavellastNameJustcreatorTypeauthorfirstNameFrantiu0161eklastNameu0160u0165u00e1hlavsku00fdabstractNoteWeinvestigatedthegenusCyphophthalmuswithinthesuborderCyphophthalmiArachnidaOpilionesfocusingonitscytogeneticevolutionintheBalkanPeninsula.Ourcomprehensiveanalysisof21speciesrevealedanotablerangeindiploidchromosomenumbers2nu2005u200524u201330challengingpreviousassumptionsofgeneticuniformitywithinthisgroup.UsingfluorescentinsituhybridizationFISHwithan18SrDNAprobewedetectedvariabilityinthenumberoflociwithevidenceofindependentincreasesinthisclusteruptofivepairs.ThisstudycombinestheresultsofphylogeneticreconstructionbasedonanalysisofmitochondrialgenecytochromecoxidasesubunitICOIincludingkaryotypedindividualswithstandardandmolecularcytogenetictechniquesmakingitthefirstofitskindinharvestmanresearch.ThefindingsrevealasignificantlyhigherlevelofbiodiversityintheBalkanPeninsulathanpreviouslyrecognizedsuggestingcomplexdifferentiationwithinthegenusCyphophthalmus.Additionallyourresultshighlighttheefficacyofcytogeneticsasatoolforspeciesu2019delineationenrichingourunderstandingoftheevolutionaryhistoryofthegenusandemphasizingtheintricategeneticdiversityshapedbytheuniquegeologicalandenvironmentalhistoryoftheBalkanPeninsula.date2025-07-01sectionpartNumberpartTitleDOI10.1093zoolinneanzlaf068citationKeyurlhttpsdoi.org10.1093zoolinneanzlaf068PMIDPMCIDISSN0024-4082languagecollectionsJRFQ2MKIdateModified2025-09-06T085821ZkeyT46XPQ63libraryid5891878metacreatorSummaryKurtoviu0107etal.parsedDate2025-06-03numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKurtovix107K.Vosolsobx11BS.Nedvx11BdD.MxFCllerK.DobrevP.I.SchmidtV.PiszczekP.KuhnA.SmoljanA.FisherT.J.WeijersD.FrimlJ.BowmanJ.L.PetrxE1x161ekJ.ltbgtTheRoleofIndole-3-AceticAcidandCharacterizationofPINTransportersinComplexStreptophyteAlgaCharaBrauniiltbgt.ltigtNewPhytologistltigtltbgt2025ltbgtltigtnaltigtna.ltaclass039zp-DOIURL039href039httpsdoi.org10.1111nph.70019039gthttpsdoi.org10.1111nph.70019ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTheroleofindole-3-aceticacidandcharacterizationofPINtransportersincomplexstreptophytealgaCharabrauniicreatorscreatorTypeauthorfirstNameKatarinalastNameKurtoviu0107creatorTypeauthorfirstNameStanislavlastNameVosolsobu011bcreatorTypeauthorfirstNameDaniellastNameNedvu011bdcreatorTypeauthorfirstNameKarellastNameMu00fcllercreatorTypeauthorfirstNamePetreIvanovlastNameDobrevcreatorTypeauthorfirstNameVojtu011bchlastNameSchmidtcreatorTypeauthorfirstNamePiotrlastNamePiszczekcreatorTypeauthorfirstNameAndrelastNameKuhncreatorTypeauthorfirstNameAdrijanalastNameSmoljancreatorTypeauthorfirstNameTomJ.lastNameFishercreatorTypeauthorfirstNameDolflastNameWeijerscreatorTypeauthorfirstNameJiu0159u00edlastNameFrimlcreatorTypeauthorfirstNameJohnL.lastNameBowmancreatorTypeauthorfirstNameJanlastNamePetru00e1u0161ekabstractNoteAuxinindole-3-aceticacidIAAisakeyphytohormonewithdiversemorphogenicrolesinlandplantsbutitsfunctionandtransportmechanismsinalgaeremainpoorlyunderstood.WethereforeaimedtoexploretheroleofIAAinacomplexstreptophytealgaeCharabraunii.HerewedescribednovelresponsesofC.brauniitoIAAandcharacterizedtwohomologsofPINauxineffluxcarriersCbPINaandCbPINc.WedeterminedtheirlocalizationinC.brauniiusingepitope-specificantibodiesandtestedtheirfunctioninheterologouslandplantmodels.FurtherusingphosphoproteomicanalysisweidentifiedIAA-inducedphosphorylationevents.ThethallusregenerationassayshowedthatIAApromotesthalluselongationandsidebranchdevelopment.ImmunolocalizationofCbPINaandCbPINcconfirmedtheirpresenceontheplasmamembraneofvegetativeandgenerativecellsofC.braunii.HoweverfunctionalassaysintobaccoBY-2cellsdemonstratedthatCbPINaaffectsauxintransportwhereasCbPINcdoesnot.TheIAAiseffectiveintheaccelerationofcytoplasmicstreamingandthephosphorylationofevolutionaryconservedtargetssuchashomologofRAF-likekinase.ThesefindingssuggestthatalthoughcanonicalPIN-mediatedauxintransportmechanismsmightnotbefullyconservedinCharaIAAisinvolvedinmorphogenesisandfastsignalingprocesses.date6.3.2025sectionpartNumberpartTitleDOI10.1111nph.70019citationKeyurlhttpsonlinelibrary.wiley.comdoiabs10.1111nph.70019PMIDPMCIDISSN1469-8137languageencollectionsJRFQ2MKIdateModified2025-03-07T070657Zkey2FU5RKZDlibraryid5891878metacreatorSummaryKampovu00e1etal.parsedDate2025-05-20numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKampovxE1A.NowackM.K.FendrychM.Vosolsobx11BS.ltbgtHygrometricallyControlledProgrammedCellDeathDrivesAntherOpeningandPollenReleaseltbgt.ltigtProceedingsoftheNationalAcademyofSciencesltigtltbgt2025ltbgtltigt122ltigt20e2420132122.ltaclass039zp-DOIURL039href039httpsdoi.org10.1073pnas.2420132122039gthttpsdoi.org10.1073pnas.2420132122ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleHygrometricallycontrolledprogrammedcelldeathdrivesantheropeningandpollenreleasecreatorscreatorTypeauthorfirstNameAnnalastNameKampovu00e1creatorTypeauthorfirstNameMoritzK.lastNameNowackcreatorTypeauthorfirstNameMatyu00e1u0161lastNameFendrychcreatorTypeauthorfirstNameStanislavlastNameVosolsobu011babstractNoteAntherdehiscenceistheprocessthatfacilitatespollenreleasefrommatureanthersninfloweringplants.Despiteitscrucialimportancetoreprodu...date20250520sectionpartNumberpartTitleDOI10.1073pnas.2420132122citationKeyurlhttpswww.pnas.orgdoiabs10.1073pnas.2420132122PMIDPMCIDISSNlanguageENcollectionsJRFQ2MKIdateModified2025-06-05T210150ZkeyWHQ8MH2Wlibraryid5891878metacreatorSummaryPoorsharbafGhavietal.parsedDate2025-04-01numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtPoorsharbafGhaviF.Kubx16FM.PetrovO.RemzovxE1M.SamantaS.Px159echJ.OpanasenkoM.ltbgtTheSupportAffectstheCatalyticConversioninThymolHydrogenationReactionltbgt.ltigtMicroporousandMesoporousMaterialsltigtltbgt2025ltbgtltigt387ltigt113507.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.micromeso.2025.113507039gthttpsdoi.org10.1016j.micromeso.2025.113507ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleThesupportaffectsthecatalyticconversioninthymolhydrogenationreactioncreatorscreatorTypeauthorfirstNameFatemelastNamePoorsharbafGhavicreatorTypeauthorfirstNameMartinlastNameKubu016fcreatorTypeauthorfirstNameOleglastNamePetrovcreatorTypeauthorfirstNameMonikalastNameRemzovu00e1creatorTypeauthorfirstNameSubhajyotilastNameSamantacreatorTypeauthorfirstNameJanlastNamePu0159echcreatorTypeauthorfirstNameMaksymlastNameOpanasenkoabstractNoteHydrogenationofthearomaticringinphenoliccompoundsisanimportantreactioninindustryandinthesynthesisoffinechemicals.Pd-supportedcatalystshaveprovedtobehighlyactiveandefficientindeephydrogenationofaromaticring.Previousstudiesconfirmedthattheacidityandtopologypropertiesofthesupportplayanimportantroleintheefficienttransformationofthephenolicreactant.InthisstudywehaveinvestigatedtheeffectofthesetwoparametersbydesigningadvancedlayeredMFIandMWWzeolites.TounderstandtheeffectofaciditywecomparedthecatalyticperformanceofaseriesofPdMFIcatalyststhatsignificantlydifferedintheacidityofthesupport.ThenwecomparedthecatalyticresultsfromPdonaluminosilicateMFIandPdMCM-56toseetheeffectofthesupport039stopology.ThymolwasusedasamodelphenolicreactantandtheresultsconfirmedthatnotonlythePdonnon-acidicMFIsupportwasinactiveconversionsbelow25u00a0butalsotheacidityoftheMFIsupportledtohigherthymolconversion47u00a0whilemoreweakacidcentersandsilanolgroupsindealuminatedMFItoppeduptheoutcome100u00a0conversion.DataalsoshowedthatbetweentwoaluminosilicatesupportstheMWWoutperformedMFI100u00a0vs.47u00a0duetoitstopologyandmorphologyforbetteraccommodatingthymolandinteractingwithit.date2025-04-01sectionpartNumberpartTitleDOI10.1016j.micromeso.2025.113507citationKeyurlhttpswww.sciencedirect.comsciencearticlepiiS1387181125000216PMIDPMCIDISSN1387-1811languagecollectionsJRFQ2MKIdateModified2025-09-06T093631ZkeyZY6FN226libraryid5891878metacreatorSummaryKollu00e1rovu00e1etal.parsedDate2025-03-01numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKollxE1rovxE1E.BaqueroForeroA.YildizA.B.Kox10DovxE1H.x17DxE1rskxFDV.Cvrx10DkovxE1F.ltbgtTheArabidopsisClassIForminAtFH5ContributestoSeedlingResistancetoSaltStressltbgt.ltigtPlantStressltigtltbgt2025ltbgtltigt15ltigt100770.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.stress.2025.100770039gthttpsdoi.org10.1016j.stress.2025.100770ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTheArabidopsisClassIforminAtFH5contributestoseedlingresistancetosaltstresscreatorscreatorTypeauthorfirstNameEvalastNameKollu00e1rovu00e1creatorTypeauthorfirstNameAneu017ekalastNameBaqueroForerocreatorTypeauthorfirstNameAliBuraklastNameYildizcreatorTypeauthorfirstNameHelenalastNameKou010dovu00e1creatorTypeauthorfirstNameViktorlastNameu017du00e1rsku00fdcreatorTypeauthorfirstNameFatimalastNameCvru010dkovu00e1abstractNoteThefamilyofforminsevolutionarilyconservedmultidomainproteinsengagedinthecontrolofactinandmicrotubulecytoskeletonorganizationexhibitsconsiderablediversityinplants.AngiospermshavetwoformincladesconsistingofmultipleparalogsClassIandClassIItheformerbeingoftentransmembraneproteinslocatedattheplasmalemmaorendomembranes.AccordingtoavailabletranscriptomedatatheArabidopsisthalianaClassItransmembraneforminAtFH5At5g54650exhibitsadistinctpatternoftranscriptabundanceinvariousseedlingroottissueswithmassiveincreaseoftranscriptleveluponsalinitystress.ToexamineapossibleroleofAtFH5inNaClstressresponsewegeneratedtransgenicplantsexpressinggreenfluorescentproteinGFP-taggedAtFH5underitsnativepromoterandcharacterizeditstissueandintracellularlocalizationunderstandardcultureconditionsandunderNaClstress.WhileweconfirmedtheinductionofAtFH5expressionbysalttreatmentthedistributionoftaggedproteinwithmaximaintheborder-likecellsoftherootcapinthephloemandatlateralrootemergencesitesdidnotreflectpreviouslyreportedtranscriptabundancesuggestingposttranscriptionalregulationofgeneexpression.SubcellularlocalizationstudiesemployingalsomembranetraffickinginhibitorssuggestedthatAtFH5proteinlevelmaybemodulatedbyendocytosisandautophagy.Notablyloss-of-functionatfh5mutantsexhibitedincreasedsensitivitytoNaClstressindicatingthatAtFH5contributestothedevelopmentofseedlingsalttolerance.ThesefindingshighlightthefunctionalimportanceofAtFH5inabioticstressresponses.date2025-03-01sectionpartNumberpartTitleDOI10.1016j.stress.2025.100770citationKeyurlhttpswww.sciencedirect.comsciencearticlepiiS2667064X25000351PMIDPMCIDISSN2667-064XlanguagecollectionsJRFQ2MKIdateModified2025-03-10T074018ZkeyW6SNS4GTlibraryid5891878metacreatorSummaryKoneu010dnu00fdetal.parsedDate2025-02-24numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKonex10DnxFDL.Jedlix10DkovxE1L.IbnahatenZ.RobertsA.CrosnierC.Dvox159xE1kJ.ltbgtEggs-PosedRevisionofSchistosomaMansoniVenomAllergen-likeProteinsUnveilsNewGenesandOffersNewInsightsintoEgg-HostInteractionsltbgt.ltigtBMCGenomicsltigtltbgt2025ltbgtltigt26ltigt1189.ltaclass039zp-ItemURL039href039httpsdoi.org10.1186s12864-025-11369-4039gthttpsdoi.org10.1186s12864-025-11369-4ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleEggs-posedrevisionofSchistosomamansonivenomallergen-likeproteinsunveilsnewgenesandoffersnewinsightsintoegg-hostinteractionscreatorscreatorTypeauthorfirstNameLuku00e1u0161lastNameKoneu010dnu00fdcreatorTypeauthorfirstNameLucielastNameJedliu010dkovu00e1creatorTypeauthorfirstNameZainalastNameIbnahatencreatorTypeauthorfirstNameAdamlastNameRobertscreatorTypeauthorfirstNameCecilelastNameCrosniercreatorTypeauthorfirstNameJanlastNameDvou0159u00e1kabstractNoteVenomallergen-likeproteinsVALsareabundantintheexcretory-secretoryproductsESPsofnumerousparasitichelminthsandhavebeenextensivelystudiedforover30u00a0yearsbecauseoftheirpotentialtointeractwithhostsystems.Despitesubstantialresearchhowevertheprecisefunctionsoftheseproteinsremainlargelyunresolved.Schistosomesparasitesofthecirculatorysystemarenoexceptionwith29SmVALgenesidentifiedinthegenomeofu00a0Schistosomamansonitodate.Theeggsoftheseparasitesasprimarypathogenicagentsinteractdirectlywithhosttissuesandreleaseexcretory-secretoryproductsthataidtheiregressfromthehost.AlthoughSmVALshavebeendetectedintheeggsecretomeinthepastdirectevidenceoftheirsecretionandfunctionalinteractionwithhostmoleculeshasneverbeendemonstrated.Thesefindingsfueltheongoingdebateastowhetheregg-expressedSmVALsinteractwiththemammalianhostorarerathermiracidialproteinssynthesizedwithintheeggduringlarvaldevelopment.date2025-02-24sectionpartNumberpartTitleDOI10.1186s12864-025-11369-4citationKeyurlhttpsdoi.org10.1186s12864-025-11369-4PMIDPMCIDISSN1471-2164languagecollectionsJRFQ2MKIdateModified2025-07-17T085412ZkeyBTDLJU8Glibraryid5891878metacreatorSummaryu0160tu011bpu00e1neketal.parsedDate2025-02-05numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtx160tx11BpxE1nekO.PariggerM.ProchxE1zkovxE1E.x10CmokovxE1A.Kolax159xEDkM.Drax10DxEDnskxE1H.x10CernxE1V.KalxEDkovxE1K.GrobxE1rovxE1V.x10CernxFDJ.SchelerJ.SchweigerG.BinderU.Baszczyx148skiO.ltbgtProdruggingFungicidalAmphotericinBSignificantlyDecreasesItsToxicEffectsltbgt.ltigtEuropeanJournalofMedicinalChemistryltigtltbgt2025ltbgtltigt283ltigt117157.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.ejmech.2024.117157039gthttpsdoi.org10.1016j.ejmech.2024.117157ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleProdruggingfungicidalamphotericinBsignificantlydecreasesitstoxiceffectscreatorscreatorTypeauthorfirstNameOndu0159ejlastNameu0160tu011bpu00e1nekcreatorTypeauthorfirstNameMarielastNamePariggercreatorTypeauthorfirstNameEliu0161kalastNameProchu00e1zkovu00e1creatorTypeauthorfirstNameAdu00e9lalastNameu010cmokovu00e1creatorTypeauthorfirstNameMiroslavlastNameKolau0159u00edkcreatorTypeauthorfirstNameHelenalastNameDrau010du00ednsku00e1creatorTypeauthorfirstNameVu011bralastNameu010cernu00e1creatorTypeauthorfirstNameKvu011btalastNameKalu00edkovu00e1creatorTypeauthorfirstNameValu00e9rialastNameGrobu00e1rovu00e1creatorTypeauthorfirstNameJanlastNameu010cernu00fdcreatorTypeauthorfirstNameJakoblastNameSchelercreatorTypeauthorfirstNameGottfriedlastNameSchweigercreatorTypeauthorfirstNameUlrikelastNameBindercreatorTypeauthorfirstNameOndu0159ejlastNameBaszczyu0148skiabstractNoteAmphotericinBAmBisoneofthemosteffectiveantifungaldrugswithastrongdose-dependentactivityagainstmostCandidaandAspergillusspeciesresponsibleforlife-threateninginfections.HoweverAmBisseverelytoxicwhichhindersitsbroaduse.Inthisproof-of-conceptstudywedemonstratethatprodruggingAmBconsiderablydecreasesAmBtoxicitywithoutaffectingitsfungicidalactivity.ForthispurposewemodifiedtheAmBstructurebyattachingadesignerphosphatepromoietytherebyswitchingoffitsmodeofactionandpreventingitstoxiceffects.TheoriginalfungicidalactivityofAmBwasthenrestoreduponprodrugactivationbyhostplasmaenzymes.TheseAmBprodrugsshowedasafertoxicityprofilethancommercialAmBdeoxycholateinCandidaandAspergillusspeciesandsignificantlyprolongedlarvalsurvivalofinfectedGalleriamellonellalarvae.Basedonthesefindingsprodruggingtoxicantifungalsmaybeaviablestrategyforbroadeningtheantifungalarsenalopeningupopportunitiesfortargetedprodrugdesign.date2025-02-05sectionpartNumberpartTitleDOI10.1016j.ejmech.2024.117157citationKeyurlhttpswww.sciencedirect.comsciencearticlepiiS0223523424010390PMIDPMCIDISSN0223-5234languagecollectionsJRFQ2MKIdateModified2025-09-06T093021ZkeyMS2G9A5Tlibraryid5891878metacreatorSummaryAkdenizetal.parsedDate2025-02-01numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtAkdenizZ.HavelkaM.StoklasaM.JimxE9nez-GonzxE1lezA.x17DxE1rskxFDV.XuF.StairsC.W.JerlstrxF6m-HultqvistJ.KolxEDskoM.ProvaznxEDkJ.SvxE4rdS.AnderssonJ.O.TachezyJ.ltbgtTheExpandedGenomeofHexamitaInflataaFree-LivingDiplomonadltbgt.ltigtSciDataltigtltbgt2025ltbgtltigt12ltigt1192.ltaclass039zp-DOIURL039href039httpsdoi.org10.1038s41597-025-04514-x039gthttpsdoi.org10.1038s41597-025-04514-xltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTheexpandedgenomeofHexamitainflataafree-livingdiplomonadcreatorscreatorTypeauthorfirstNameZeyneplastNameAkdenizcreatorTypeauthorfirstNameMichallastNameHavelkacreatorTypeauthorfirstNameMichallastNameStoklasacreatorTypeauthorfirstNameAlejandrolastNameJimu00e9nez-Gonzu00e1lezcreatorTypeauthorfirstNameVojtu011bchlastNameu017du00e1rsku00fdcreatorTypeauthorfirstNameFeifeilastNameXucreatorTypeauthorfirstNameCourtneyW.lastNameStairscreatorTypeauthorfirstNameJonlastNameJerlstru00f6m-HultqvistcreatorTypeauthorfirstNameMartinlastNameKolu00edskocreatorTypeauthorfirstNameJanlastNameProvaznu00edkcreatorTypeauthorfirstNameStaffanlastNameSvu00e4rdcreatorTypeauthorfirstNameJanO.lastNameAnderssoncreatorTypeauthorfirstNameJanlastNameTachezyabstractNoteDiplomonadsareanaerobicflagellatedprotistsbeingpartoftheMetamonadagroupofEukaryotes.Diplomonadseitherliveasendobiontsparasitesandcommensalsofanimalsorfree-livinginlow-oxygenenvironments.GenomicinformationisavailableforparasiticdiplomonadslikeGiardiaintestinalisandSpironucleussalmonicidawhilelittleisknownaboutthegenomicarrangementsoffree-livingdiplomonads.Wehavegeneratedthefirstreferencegenomeofafree-livingdiplomonadHexamitainflata.Thefinalversionofthegenomeassemblyisfragmented1241contigsbutsubstantiallylarger142Mbpthantheparasiticdiplomonadgenomes9.8u201314.7Mbp.Itencodes79341proteins29874havefunctionalannotationsand49467arehypotheticalproteins.Interspersedrepeatscomprise34ofthegenome9617Retroelements2676DNAtransposons.Thelargeexpansionofprotein-encodingcapacityandtheinterspersedrepeatsarethemajorreasonsforthelargegenomesize.Thisgenomefromafree-livingdiplomonadwillbethebasisforfurtherstudiesoftheDiplomonadidalineageandtheevolutionofparasitism-freelivingstyletransitions.date2025-02-01sectionpartNumberpartTitleDOI10.1038s41597-025-04514-xcitationKeyurlhttpswww.nature.comarticless41597-025-04514-xPMIDPMCIDISSN2052-4463languageencollectionsJRFQ2MKIdateModified2025-09-06T091802ZkeyIYCRJSCClibraryid5891878metacreatorSummaryKraletal.parsedDate2025-02numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKralJ.SemberA.DivisovaK.KorinkovaT.ReyesLermaA.C.avilaHerreraI.M.FormanM.StahlavskyF.MusilovaJ.TorresKalmeS.PalaciosVargasJ.G.ZrzavaM.VrbovaI.Moreno-GonzalezJ.A.CushingP.E.GromovA.V.SebestianovaS.SlechtovaV.B.PrendiniL.BirdT.L.ltbgtAdvancesinUnderstandingtheKaryotypeEvolutionofTetrapulmonataandTwoOtherArachnidTaxaRicinuleiandSolifugaeltbgt.ltigtGenesltigtltbgt2025ltbgtltigt16ltigt2207.ltaclass039zp-DOIURL039href039httpsdoi.org10.3390genes16020207039gthttpsdoi.org10.3390genes16020207ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleAdvancesinUnderstandingtheKaryotypeEvolutionofTetrapulmonataandTwoOtherArachnidTaxaRicinuleiandSolifugaecreatorscreatorTypeauthorfirstNameJirilastNameKralcreatorTypeauthorfirstNameAlexandrlastNameSembercreatorTypeauthorfirstNameKlaralastNameDivisovacreatorTypeauthorfirstNameTerezalastNameKorinkovacreatorTypeauthorfirstNameAzucenaC.lastNameReyesLermacreatorTypeauthorfirstNameIvaluM.lastNameavilaHerreracreatorTypeauthorfirstNameMartinlastNameFormancreatorTypeauthorfirstNameFrantiseklastNameStahlavskycreatorTypeauthorfirstNameJanalastNameMusilovacreatorTypeauthorfirstNameSabrinalastNameTorresKalmecreatorTypeauthorfirstNameJoseG.lastNamePalaciosVargascreatorTypeauthorfirstNameMagdalastNameZrzavacreatorTypeauthorfirstNameIvalastNameVrbovacreatorTypeauthorfirstNameJairoA.lastNameMoreno-GonzalezcreatorTypeauthorfirstNamePaulaE.lastNameCushingcreatorTypeauthorfirstNameAlexanderV.lastNameGromovcreatorTypeauthorfirstNameStepankalastNameSebestianovacreatorTypeauthorfirstNameVendulaBohlenlastNameSlechtovacreatorTypeauthorfirstNameLorenzolastNamePrendinicreatorTypeauthorfirstNameTharinaL.lastNameBirdabstractNoteBackgroundObjectivesArachnidsareamegadiversearthropodgroup.ThepresentstudyinvestigatedthechromosomesofpedipalpidtetrapulmonatesordersAmblypygiThelyphonidaSchizomidaandtwoarachnidordersofuncertainphylogeneticplacementRicinuleiandSolifugaetoreconstructtheirkaryotypeevolution.Exceptforamblypygidsthecytogeneticsofthesearachnidorderswasalmostunknownpriortothepresentstudy.MethodsChromosomeswereinvestigatedusingmethodsofstandardGiemsa-stainedpreparationsbandingtechniquesandmolecularcytogeneticsfluorescenceinsituhybridizationcomparativegenomichybridization.ResultsandConclusionsNewdatafor38speciescombinedwithpreviouslypublisheddatasuggestthatancestralarachnidspossessedlowtomoderate2n22-40monocentricchromosomesonenucleolusorganizerregionNORlowlevelsofheterochromatinandrecombinationsandnoorhomomorphicsexchromosomes.KaryotypesofPedipalpiandSolifugaediversifiedviacentricfusionspericentricinversionsandchangesinthepatternofNORsandinsolifugesalsothroughtandemfusions.SomesolifugesdisplayanenormousamountofconstitutiveheterochromatinandhighNORnumber.ItishypothesizedthatthecommonancestorofamblypygidsthelyphonidsandspidersexhibitedahomomorphicXYsystemandthattelomericheterochromatinandNORswereinvolvedintheevolutionofamblypygidsexchromosomes.ThenewfindingssupporttheCephalosomatacladeacariformspalpigradesandsolifuges.Hypothesesconcerningtheoriginofacariformholocentricchromosomesarepresented.UnlikecurrentphylogenetichypothesestheresultssuggestasisterrelationshipbetweenSchizomidaandacladecomprisingothertetrapulmonatesaswellasapolyploidizationinthecommonancestorofthecladecomprisingAraneaeAmblypygiandThelyphonida.dateFEB2025sectionpartNumberpartTitleDOI10.3390genes16020207citationKeyurlPMIDPMCIDISSN2073-4425languageEnglishcollectionsJRFQ2MKIdateModified2025-03-19T115834ZkeyBVLSQE5Elibraryid5891878metacreatorSummaryHudacovaetal.parsedDate2025-01-01numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHudacovaE.AbaffyP.KaplanM.M.KrausovaM.KubistaM.MachonO.ltbgtSingle-CellTranscriptomicResolutionofOsteogenesisduringCraniofacialMorphogenesisltbgt.ltigtBoneltigtltbgt2025ltbgtltigt190ltigt117297.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.bone.2024.117297039gthttpsdoi.org10.1016j.bone.2024.117297ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleSingle-celltranscriptomicresolutionofosteogenesisduringcraniofacialmorphogenesiscreatorscreatorTypeauthorfirstNameErikalastNameHudacovacreatorTypeauthorfirstNamePavellastNameAbaffycreatorTypeauthorfirstNameMehmetMahsumlastNameKaplancreatorTypeauthorfirstNameMichaelalastNameKrausovacreatorTypeauthorfirstNameMikaellastNameKubistacreatorTypeauthorfirstNameOndrejlastNameMachonabstractNoteCraniofacialmorphogenesisdependsoncomplexcellfatedecisionsduringthedifferentiationofpost-migratorycranialneuralcrestcells.Molecularmechanismsofcelldifferentiationofmesenchymalcellstodevelopingbonescartilageteethtongueandothercraniofacialtissuesarestillpoorlyunderstood.Weperformedsingle-celltranscriptomicanalysisofcraniofacialmesenchymalcellsderivedfromcranialNCCsinmouseembryo.UsingFACSsortingofWnt1-Cre2progenywecarefullymappedthecellheterogeneityinthecraniofacialregionduringtheinitialstagesofcartilageandboneformation.Transcriptomicdataandinvivovalidationsidentifiedmoleculardeterminantsofmajorcellpopulationsinvolvedinthedevelopmentoflowerandupperjawteethtonguedermisorperiocularmesenchyme.Single-celltranscriptomicanalysisofMeis2-deficientmicerevealedcriticalgeneexpressiondifferencesincludingincreasedosteogenicandcelladhesionmarkers.Thisleadstoaffectedmesenchymalcelldifferentiationandincreasedossificationresultinginimpairedbonecartilageandtongueformation.date2025-01-01sectionpartNumberpartTitleDOI10.1016j.bone.2024.117297citationKeyurlhttpswww.sciencedirect.comsciencearticlepiiS8756328224002862PMIDPMCIDISSN8756-3282languagecollectionsJRFQ2MKIdateModified2025-09-06T093557Zkey7CGYDYP3libraryid5891878metacreatorSummaryKhromykhetal.parsedDate2025numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKhromykhN.O.DidurO.O.SklyarT.ProchazkovaL.NedbalovaL.NebesarovaJ.BalalaievO.K.KuraginaN.ltbgtCharacterizationofSorbusAria-MediatedSilverNanoparticlesandEvaluationofAntibacterialActivityagainstStaphylococcusEpidermidisClinicalStrainsltbgt.ltigtRegul.Mech.Biosyst.ltigtltbgt2025ltbgtltigt16ltigt2e25056.ltaclass039zp-DOIURL039href039httpsdoi.org10.154210225056039gthttpsdoi.org10.154210225056ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleCharacterizationofSorbusaria-mediatedsilvernanoparticlesandevaluationofantibacterialactivityagainstStaphylococcusepidermidisclinicalstrainscreatorscreatorTypeauthorfirstNameN.O.lastNameKhromykhcreatorTypeauthorfirstNameO.O.lastNameDidurcreatorTypeauthorfirstNameT.lastNameSklyarcreatorTypeauthorfirstNameL.lastNameProchazkovacreatorTypeauthorfirstNameL.lastNameNedbalovacreatorTypeauthorfirstNameJ.lastNameNebesarovacreatorTypeauthorfirstNameO.K.lastNameBalalaievcreatorTypeauthorfirstNameN.lastNameKuraginaabstractNoteBiosynthesisofsilvernanoparticlesAgNPsusingplantextractsservesasanattractiveenvironmentallyfriendlyandcosteffectivealternativetothetraditionalmethodsofproducingnanomaterialswithantimicrobialproperties.ThisarticlecharacterizesAgNPsderivedfromanaqueousleafextractofSorbusariaplantsSa-AgNPsandpresentstheresultsoftheirtestingagainstantibiotic-resistantclinicalbacterialstrains.TheformationofsilvernanoparticleswasobservedvisuallyaccordingtothechangeinthecolorofsolutionandwasconfirmedbytheinducedplasmonresonancepeakrecordedbyUV-Visspectroscopyat450nm.AccordingtotheTEMimagingthebiosynthesizedSa-AgNPsweresphericalwithanaveragesizeof47.5nmandexhibitedamoderatepolydispersitywiththePDIcalculatedas0.138.TheSEMimagesconfirmedthesphericalshapeoftheSa-AgNPsandtheabsenceoftheiragglomeration.ThephytochemicalsfromtheplantmatrixthatservedasreducingstabilizingandcappingagentsforSa-AgNPsbiosynthesiswereidentifiedusingFouriertransforminfraredspectroscopyasphenolicsalcoholsterpenesandproteinswithhydroxylcarbonylcarboxylandaminesbeingtheresponsiblefunctionalgroups.TheantibacterialactivityofthebiosynthesizedsilvernanoparticleswasexaminedusingthediscdiffusionmethodagainsttwoclinicallyisolatedStaphylococcusepidermidisstrainsthatdifferedinsensitivitytosomeantibioticsfromseveralclasses.ThegrowthinhibitionofbothS.epidermidisstrainsbySa-AgNPswasdose-dependentataconcentrationrangeof15.625-1000mugmL.TheS.epidermidisstrainthatdisplayedastrongerresistancetoseveralfluoroquinolonescephalosporinesandaminoglycosidesshowedhighersusceptibilitytotheantibacterialactionofSa-AgNPsthanthelessdrug-resistantstrain.ThehemolyticassayrevealedagoodbiologicalcompatibilityofthebiosynthesizedSa-AgNPsatconcentrationsofupto25mugmL.ThestudyresultsconfirmedthepotentialabilityofphytosynthesizedsilvernanoparticlestoachieveconsiderablesuccessinthefightagainstantibioticresistanceofS.epidermidis.FurtherresearchisneededtotestalargenumberofresistantclinicalstrainstoclarifythesuitabilityofSa-AgNPsfordevelopmentofnewantibacterialdrugs.date2025sectionpartNumberpartTitleDOI10.154210225056citationKeyurlPMIDPMCIDISSN2519-85212520-2588languageEnglishcollectionsJRFQ2MKIdateModified2026-04-20T064143ZkeyLBUE5NKVlibraryid5891878metacreatorSummaryVosu00e1laetal.parsedDate2025numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtVosxE1laO.x160mxEDdovxE1B.NovxE1kJ.SvobodaJ.PetrxE1sekT.Vojtx11BchovxE1I.MachxE1x10DekT.ltbgtNoEvidenceofAlzheimerx2019sDiseasePathologyinMiceInfectedwithToxocaraCanisltbgt.ltigtParasiteltigtltbgt2025ltbgtltigt32ltigt24.ltaclass039zp-DOIURL039href039httpsdoi.org10.1051parasite2025019039gthttpsdoi.org10.1051parasite2025019ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleNoevidenceofAlzheimeru2019sdiseasepathologyinmiceinfectedwithToxocaracaniscreatorscreatorTypeauthorfirstNameOndu0159ejlastNameVosu00e1lacreatorTypeauthorfirstNameBarboralastNameu0160mu00eddovu00e1creatorTypeauthorfirstNameJanlastNameNovu00e1kcreatorTypeauthorfirstNameJanlastNameSvobodacreatorTypeauthorfirstNameTomu00e1u0161lastNamePetru00e1sekcreatorTypeauthorfirstNameIvetalastNameVojtu011bchovu00e1creatorTypeauthorfirstNameTomu00e1u0161lastNameMachu00e1u010dekabstractNoteThepotentiallinkbetweentheinfectionsandthedevelopmentofAlzheimeru2019sdiseaseADhasledtospeculationsabouttheroleofvariouspathogensintriggeringamyloid-u03b2Au03b2overproductionpossiblyleadingtoADonset.ThegloballydistributeddogroundwormltigtToxocaracanisltigtwassuggestedtobeasuitablecandidateduetoneurotropismofthelarvaeandinfectionchronicity.ThisstudyinvestigatedwhetherchronicltigtT.canisltigtinfectioninducesAD-likepathologyinmiceandwhetherAu03b2istoxictoltigtT.canisltigt.BALBcandAPPPS1transgenicmicewhichoverproduceAu03b2wereinfectedwithltigtT.canisltigtL3larvaeandmonitoredforlarvalburdenAu03b2accumulationandbehavioralchanges.ltigtInvitroltigttestsofrecombinantAu03b2toxicityagainstthelarvaewerealsoperformed.DespitethepresenceofltigtT.canisltigtlarvaeinthecentralnervoussystem8and16weekspost-infectionnosignificantincreaseinAu03b2concentrationorAD-relatedbehavioralalterationswereobserved.Au03b2wasdetectedonthesurfaceandwithintheintestinesofltigtT.canisltigtlarvaebutltigtinvitroltigtexposuretorecombinantAu03b2didnotaffectlarvalviabilityormorphology.OurfindingssuggestthatltigtT.canisltigtinfectiondoesnottriggerAD-likepathologyinmiceandAu03b2doesnotactasanantiparasiticagent.ThischallengestheemerginghypothesisthatchronicneurotoxocarosisinfectionsmaycontributetoADdevelopment.date2025sectionpartNumberpartTitleDOI10.1051parasite2025019citationKeyurlhttpswww.parasite-journal.orgarticlesparasiteabs202501parasite250018parasite250018.htmlPMIDPMCIDISSN1776-1042languageencollectionsJRFQ2MKIdateModified2025-04-24T140417ZkeyZNK2QD6Xlibraryid5891878metacreatorSummaryu0158ezu00e1u010detal.parsedDate2025numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtx158ezxE1x10DM.KrxE1lJ.xC1vilaHerreraI.M.FormanM.x158ezxE1x10DovxE1V.GlorxEDkovxE1N.HenebergP.ltbgtDysderaParthenogeneticaSp.Nov.AraneaeDysderidaeAUniqueCaseofParthenogenesisinSpidersltbgt.ltigtJournalofZoologicalSystematicsandEvolutionaryResearchltigtltbgt2025ltbgtltigt2025ltigt19266860.ltaclass039zp-DOIURL039href039httpsdoi.org10.1155jzs9266860039gthttpsdoi.org10.1155jzs9266860ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDysderaparthenogeneticasp.nov.AraneaeDysderidaeAUniqueCaseofParthenogenesisinSpiderscreatorscreatorTypeauthorfirstNameMilanlastNameu0158ezu00e1u010dcreatorTypeauthorfirstNameJiu0159u00edlastNameKru00e1lcreatorTypeauthorfirstNameIvalu00faMacarenalastNameu00c1vilaHerreracreatorTypeauthorfirstNameMartinlastNameFormancreatorTypeauthorfirstNameVeronikalastNameu0158ezu00e1u010dovu00e1creatorTypeauthorfirstNameNelalastNameGloru00edkovu00e1creatorTypeauthorfirstNamePetrlastNameHenebergabstractNoteWestudiedtheparthenogeneticlineagesofthespiderDysderahungaricaAraneaeDysderidae.BasedonourdataweconsiderthemtoconstituteaseparatetaxonDysderaparthenogeneticasp.nov.Morphologicallythenewspeciesdiffersmainlybyslightlyreducedfemalecopulatoryorgans.Theovariescontainmeioticcellssuggestingthatautomicticthelytokyoccursinthisspecies.D.parthenogeneticasp.nov.colonisedareaswestoftheancestralsexualspeciesD.hungaricaespeciallythePannonianregionthedistributionareasofthesespeciesshowminimaloverlap.ThedistributionpatternofD.parthenogeneticasp.nov.suggeststhattheobligatethelytokyinthisspeciesoriginatedthroughgeographicthelytoky.D.parthenogeneticasp.nov.hasbeenfoundinasignificantlylargervarietyofhabitatsthanD.hungaricaincludingagroecosystems.ThereforetheparthenogenesisofD.parthenogeneticasp.nov.isassociatedwiththeabilitytopopulateevenhabitatswithouttreeorbushcoveroftendisturbedwhichisunfavourableforotherDysderaspecies.AccordingtotheanalysisofselectednuclearITS2andmitochondrialmarkersCOID.parthenogeneticasp.nov.showedlowgeneticdiversitysingleCOIhaplotypeandtwocloselyrelatedITS2haplotypesincontrasttotheancestralD.hungarica.ByseparationofD.parthenogeneticasp.nov.D.hungaricabecomesaparaphyleticspecies.D.hungaricaisthusoneofthefirstdocumentedcasesofparaspeciesamongspiders.AlthoughD.parthenogeneticasp.nov.exhibitsminimumgeneticvariationattheanalysedmolecularmarkersitdisplaysconsiderablekaryotypediversity.Thetransitiontoparthenogenesiswasaccompaniedbyadecreaseindiploidnumberthroughchromosomefusions.KaryotypesofD.parthenogeneticasp.nov.divergedconsiderablyfromthoseofD.hungarica.Potentialhybridsbetweenthesespecieswouldlikelyproducegameteswithdefectivegenomes.Thereisalsoabehaviouralbarrierbetweenthesetwotaxa.FemalesofD.parthenogeneticasp.nov.refusetomate.date2025sectionpartNumberpartTitleDOI10.1155jzs9266860citationKeyurlhttpsonlinelibrary.wiley.comdoiabs10.1155jzs9266860PMIDPMCIDISSN1439-0469languageencollectionsJRFQ2MKIdateModified2025-11-07T091437Zkey5TCMMJT3libraryid5891878metacreatorSummaryLastovickovu00e1etal.parsedDate2025numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtLastovickovxE1A.RainerD.N.MazurM.ltbgtSheddingElectronsonADORZeoliteStructures-StructureDeterminationby3DEDltbgt.ltigtMICROPOROUSANDMESOPOROUSMATERIALSltigtltbgt2025ltbgtltigt387ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.micromeso.2025.113514039gthttpsdoi.org10.1016j.micromeso.2025.113514ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleSheddingelectronsonADORzeolitestructures-Structuredeterminationby3DEDcreatorscreatorTypeauthorfirstNameA.lastNameLastovickovu00e1creatorTypeauthorfirstNameD.N.lastNameRainercreatorTypeauthorfirstNameM.lastNameMazurabstractNoteThree-dimensionalelectrondiffraction3DEDoffersapowerfulalternativetosingle-crystalX-raydiffractionSCXRDforthestructuredeterminationofcrystallinematerialswithsmall-sizedcrystalsusuallynanometresizedorcrystalswithintergrowthsoftenproducedduringzeolitesynthesis.ADORisasynthesisstrategyofnoveldaughterzeolitesfromwell-knownparentgermanosilicates.StructuredeterminationofsuchADORzeolitesischallengingduetothepresenceofaggregatedintergrowthsandoftensmall-sizedcrystals.ThisworkdemonstratestheapplicationofcontinuousrotationelectrondiffractioncREDatypeof3DEDfortherapidstructuralcharacterisationofzeolitesproducedbyADORapproach.cREDcanbeperformedinatransmissionelectronmicroscopeandthestructureofastudiedmaterialcanbeobtainedrapidly.WeproposeaunifiedworkflowforthestructuredeterminationofADORdaughtermaterials.Thisworkflowinvolvestheinitialstructuralinvestigationanddeterminationoftheparentmaterialfollowedbytheutilizationofthisknowledgetofacilitatethesubsequentstructuredeterminationofthedaughtermaterial.InvestigationstrategywasshowntobeuniformaspresentedforADORtransformationoftwodifferentparentgermanosilicates.ThisapproachisatoolallowingpromptrecognitionofnewADORzeolitesattheearlystageoftheirsynthesisandcanbeusedforafeedback-basedoptimisationofsynthesisconditions.date2025sectionpartNumberpartTitleDOI10.1016j.micromeso.2025.113514citationKeyurlPMIDPMCIDISSN1387-1811languagecollectionsJRFQ2MKIdateModified2025-03-19T115834ZkeyPP8PRXRYlibraryid5891878metacreatorSummaryBoguschetal.parsedDate2025numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtBoguschP.Petx159xEDkO.HlavxE1x10DekA.x160ebestaO.x160xEDpekP.ltbgtThermalMelanisminPachnodaIskuulkaColeopteraScarabaeidaeCetoniinaeltbgt.ltigtInsectsltigtltbgt2025ltbgtltigt16ltigt1.ltaclass039zp-DOIURL039href039httpsdoi.org10.3390insects16010061039gthttpsdoi.org10.3390insects16010061ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleThermalMelanisminPachnodaiskuulkaColeopteraScarabaeidaeCetoniinaecreatorscreatorTypeauthorfirstNamePetrlastNameBoguschcreatorTypeauthorfirstNameOtolastNamePetu0159u00edkcreatorTypeauthorfirstNameAntonu00ednlastNameHlavu00e1u010dekcreatorTypeauthorfirstNameOndu0159ejlastNameu0160ebestacreatorTypeauthorfirstNamePetrlastNameu0160u00edpekabstractNoteThermalpolymorphismusuallyrepresentedbythermalmelanismdarkercolorationincoolerhabitatsisawell-knownphenomenoninanimals.InCetoniinaeseveralspeciesincaptivitytendtobecomedarkerafterseveralgenerationsofbreedingwhichisprobablycausedbyalowertemperaturethanistypicalfortheirnativehabitats.PachnodaiskuulkaisabeetlespeciesoccurringinSomaliland.Thisspeciesiseasytobreedincaptivityanditiscolorfulandvariableintheproportionsofyellowredandblackcoloration.Wekeptthisspeciesfromthefirstinstarlarvatotheadultstageatthreedifferenttemperatures.Elytraandpronotumoftheadultswerephotographedandproportionsofthethreemaincolorsweremeasured.Theproportionofblackcolorationsignificantlyincreasedwithsizeanddecreasedwithtemperaturewhiletheproportionofyellowcolorincreased.Thisspeciesiscertainlythermallypolymorphicwhichcanbeanadaptationforactivationevenatlowertemperatures.ThepossiblemimicrywithbeetlesofthegenusHycleusisdiscussed.ItisthefirstconfirmationofthermalpolymorphisminCetoniinaeandoneofafewinColeoptera.date2025sectionpartNumberpartTitleDOI10.3390insects16010061citationKeyurlhttpswww.mdpi.com2075-445016161PMIDPMCIDISSN2075-4450languagecollectionsJRFQ2MKIdateModified2025-03-19T115834Z
1.
Kučerová, J.; Zdrha, A.; Rozbeský, D.; Shinde, A. P.; Nebesářová, J.; Narayanasamy, R. K.; Smutná, T.; Hrdý, I.; Tachezy, J. Characterization of the sTim/MIA Pathway in Metamonada Reveals Different Evolutionary Adaptations to Anaerobiosis. Current Biology 2025, 35 (23), 5734-5749.e6. https://doi.org/10.1016/j.cub.2025.10.027.
1.
Bourland, W.; Pomahač, O.; Méndez-Sánchez, D.; Beinart, R. A.; Bernhard, J. M.; Čepička, I.; Rotterová, J. The End of a Winding Path: The Anaerobic Ciliate Spirorhynchus Is a Member of the Class Muranotrichea. Protist 2025, 179, 126129. https://doi.org/10.1016/j.protis.2025.126129.
1.
Škaloud, P.; Tučková, K.; Čablová, R.; Jadrná, I.; Černajová, I. High‐frequency Sampling Unveils Biotic and Abiotic Drivers of Rapid Phytoplankton Morphological Changes. New Phytol 2025, 248 (5), 2528–2541. https://doi.org/10.1111/nph.70534.
1.
Háček, J.; Vlková, A.; Krsek, D.; Kolařík, L.; Spálenková, A.; Sychra, T.; Tesařová, T.; Gayibov, E.; Václavíková, R.; Zieg, J.; Bielaszewska, M. Enterohemorrhagic Escherichia Coli O157 Outer Membrane Vesicles Administered by Oral Gavage Cause Renal Tubular Injury and Acute Kidney Failure in Mice. Front. Cell. Infect. Microbiol. 2025, 15. https://doi.org/10.3389/fcimb.2025.1704731.
1.
Škaloud, P.; Škaloudová, M.; Knotek, P.; Jadrná, I.; Pusztai, M. Mallomonas Intermedia: A Case Study of Speciation and Evolutionary Dynamics of Protists. Fottea 2025, 25 (2), 169–182. https://doi.org/10.5507/fot.2025.008.
1.
Corbat, A. A.; Walther, C. G.; Ballina, L. R. de la; Condon, N. D.; Felder, A. A.; Schätz, M.; Schmerl, B.; Sugawara, K.; Prats, C.; Klemm, A.; Levet, F.; Miura, K.; Sampaio, P.; Tischer, C.; D’Antuono, R.; Cimini, B. A.; Haase, R. GloBIAS: Strengthening the Foundations of BioImage Analysis. arXiv July 8, 2025. https://doi.org/10.48550/arXiv.2507.06407.
1.
Hiřman, M.; Karaman, I.; Štundlová, J.; Just, P.; Šťáhlavský, F. Evolutionary Dynamics of the Chromosomal Changes in the Genus Cyphophthalmus (Arachnida: Opiliones) on the Balkan Peninsula. Zool J Linn Soc 2025, 204 (3), zlaf068. https://doi.org/10.1093/zoolinnean/zlaf068.
1.
Kurtović, K.; Vosolsobě, S.; Nedvěd, D.; Müller, K.; Dobrev, P. I.; Schmidt, V.; Piszczek, P.; Kuhn, A.; Smoljan, A.; Fisher, T. J.; Weijers, D.; Friml, J.; Bowman, J. L.; Petrášek, J. The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii. New Phytologist 2025, n/a (n/a). https://doi.org/10.1111/nph.70019.
1.
Kampová, A.; Nowack, M. K.; Fendrych, M.; Vosolsobě, S. Hygrometrically Controlled Programmed Cell Death Drives Anther Opening and Pollen Release. Proceedings of the National Academy of Sciences 2025, 122 (20), e2420132122. https://doi.org/10.1073/pnas.2420132122.
1.
Poorsharbaf Ghavi, F.; Kubů, M.; Petrov, O.; Remzová, M.; Samanta, S.; Přech, J.; Opanasenko, M. The Support Affects the Catalytic Conversion in Thymol Hydrogenation Reaction. Microporous and Mesoporous Materials 2025, 387, 113507. https://doi.org/10.1016/j.micromeso.2025.113507.
1.
Kollárová, E.; Baquero Forero, A.; Yildiz, A. B.; Kočová, H.; Žárský, V.; Cvrčková, F. The Arabidopsis Class I Formin AtFH5 Contributes to Seedling Resistance to Salt Stress. Plant Stress 2025, 15, 100770. https://doi.org/10.1016/j.stress.2025.100770.
1.
Konečný, L.; Jedličková, L.; Ibnahaten, Z.; Roberts, A.; Crosnier, C.; Dvořák, J. Eggs-Posed: Revision of Schistosoma Mansoni Venom Allergen-like Proteins Unveils New Genes and Offers New Insights into Egg-Host Interactions. BMC Genomics 2025, 26 (1), 189. https://doi.org/10.1186/s12864-025-11369-4.
1.
Štěpánek, O.; Parigger, M.; Procházková, E.; Čmoková, A.; Kolařík, M.; Dračínská, H.; Černá, V.; Kalíková, K.; Grobárová, V.; Černý, J.; Scheler, J.; Schweiger, G.; Binder, U.; Baszczyňski, O. Prodrugging Fungicidal Amphotericin B Significantly Decreases Its Toxic Effects. European Journal of Medicinal Chemistry 2025, 283, 117157. https://doi.org/10.1016/j.ejmech.2024.117157.
1.
Akdeniz, Z.; Havelka, M.; Stoklasa, M.; Jiménez-González, A.; Žárský, V.; Xu, F.; Stairs, C. W.; Jerlström-Hultqvist, J.; Kolísko, M.; Provazník, J.; Svärd, S.; Andersson, J. O.; Tachezy, J. The Expanded Genome of Hexamita Inflata, a Free-Living Diplomonad. Sci Data 2025, 12 (1), 192. https://doi.org/10.1038/s41597-025-04514-x.
1.
Kral, J.; Sember, A.; Divisova, K.; Korinkova, T.; Reyes Lerma, A. C.; avila Herrera, I. M.; Forman, M.; Stahlavsky, F.; Musilova, J.; Torres Kalme, S.; Palacios Vargas, J. G.; Zrzava, M.; Vrbova, I.; Moreno-Gonzalez, J. A.; Cushing, P. E.; Gromov, A. V.; Sebestianova, S.; Slechtova, V. B.; Prendini, L.; Bird, T. L. Advances in Understanding the Karyotype Evolution of Tetrapulmonata and Two Other Arachnid Taxa, Ricinulei and Solifugae. Genes 2025, 16 (2), 207. https://doi.org/10.3390/genes16020207.
1.
Hudacova, E.; Abaffy, P.; Kaplan, M. M.; Krausova, M.; Kubista, M.; Machon, O. Single-Cell Transcriptomic Resolution of Osteogenesis during Craniofacial Morphogenesis. Bone 2025, 190, 117297. https://doi.org/10.1016/j.bone.2024.117297.
1.
Khromykh, N. O.; Didur, O. O.; Sklyar, T.; Prochazkova, L.; Nedbalova, L.; Nebesarova, J.; Balalaiev, O. K.; Kuragina, N. Characterization of Sorbus Aria-Mediated Silver Nanoparticles and Evaluation of Antibacterial Activity against Staphylococcus Epidermidis Clinical Strains. Regul. Mech. Biosyst. 2025, 16 (2), e25056. https://doi.org/10.15421/0225056.
1.
Vosála, O.; Šmídová, B.; Novák, J.; Svoboda, J.; Petrásek, T.; Vojtěchová, I.; Macháček, T. No Evidence of Alzheimer’s Disease Pathology in Mice Infected with Toxocara Canis. Parasite 2025, 32, 24. https://doi.org/10.1051/parasite/2025019.
1.
Řezáč, M.; Král, J.; Ávila Herrera, I. M.; Forman, M.; Řezáčová, V.; Gloríková, N.; Heneberg, P. Dysdera Parthenogenetica Sp. Nov. (Araneae, Dysderidae): A Unique Case of Parthenogenesis in Spiders. Journal of Zoological Systematics and Evolutionary Research 2025, 2025 (1), 9266860. https://doi.org/10.1155/jzs/9266860.
1.
Lastovicková, A.; Rainer, D. N.; Mazur, M. Shedding Electrons on ADOR Zeolite Structures - Structure Determination by 3DED. MICROPOROUS AND MESOPOROUS MATERIALS 2025, 387. https://doi.org/10.1016/j.micromeso.2025.113514.
1.
Bogusch, P.; Petřík, O.; Hlaváček, A.; Šebesta, O.; Šípek, P. Thermal Melanism in Pachnoda Iskuulka (Coleoptera: Scarabaeidae: Cetoniinae). Insects 2025, 16 (1). https://doi.org/10.3390/insects16010061.
2024
5891878
Q65THHXG
1
https://raw.githubusercontent.com/Schebique/vmcf-konfmi/refs/heads/main/vmcf-web-style.csl
50
date
desc
4983
https://web.natur.cuni.cz/sekce-bi/VMCF/wp-content/plugins/zotpress/
statussuccessupdateneededfalseinstancefalsemetarequest_last0request_next0used_cachetruedatakey9E48LKZZlibraryid5891878metacreatorSummaryZahradnu00edu010dkovu00e1etal.parsedDate2024-12numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtZahradnxEDx10DkovxE1V.Hovox159xE1kovxE1M.TuckerA.S.Bartox161M.RehxE1kI.ZahradnxEDx10DekO.ltbgtPostnatalDentitionChangesintheCubanFalseChameleonsAdaptationtoaDietaryShiftltbgt.ltigtfozo.1ltigtltbgt2024ltbgtltigt73ltigt2406324063.1-20.ltaclass039zp-DOIURL039href039httpsdoi.org10.25225jvb.24063039gthttpsdoi.org10.25225jvb.24063ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitlePostnataldentitionchangesintheCubanfalsechameleonsadaptationtoadietaryshiftcreatorscreatorTypeauthorfirstNameVeronikalastNameZahradnu00edu010dkovu00e1creatorTypeauthorfirstNameMu00e1rialastNameHovou0159u00e1kovu00e1creatorTypeauthorfirstNameAbigailS.lastNameTuckercreatorTypeauthorfirstNameMartinlastNameBartou0161creatorTypeauthorfirstNameIvanlastNameRehu00e1kcreatorTypeauthorfirstNameOldu0159ichlastNameZahradnu00edu010dekabstractNoteThecapacitytoproducemultiplegenerationsofteethallowsforchangesindentitionmorphologythroughoutananimal039slife.Thisadaptationcanaccommodatedifferentdietarystrategiesatdifferentlifestagesmostnotablybetweenjuvenileandadultstages.Commonchangesbetweentoothgenerationsincludevariationsintoothnumberandmorphologicalparameters.InthisstudywecomparethedentitionmorphologyofjuvenileandadultspecimensfromfourspeciesofCubanfalsechameleonsinthegenusAnolisChamaeleolisgroupwithintheIguaniacladeofsquamatereptiles.Unlikemostanolelizardswhichareprimarilyinsectivorousorsaurivorousthesespeciesarespecialisedfeedersonshelledsnailsandpossessdistinctivebroadmolariformteethatthebackoftheirjaws.Theexactdietarypreferencesofthesespeciesremainunclear.Ourfindingsrevealthatjuvenilesdifferfromadultsbythepresenceoftricuspidteethatthebackofthejawandthattheexaminedspeciesexhibitdifferencesindentitionsuchastoothnumbershapearrangementofcuspsandcrestspresenceofmolariformteethandinternaltoothstructure.Basedontheseobservationswediscusshowinterspecificandage-relatedchangesindentitionmayreflectdifferencesindiet.date202412sectionpartNumberpartTitleDOI10.25225jvb.24063citationKeyurlhttpsbioone.orgjournalsjournal-of-vertebrate-biologyvolume-73issue-24063jvb.24063Postnatal-dentition-changes-in-the-Cuban-false-chameleons--adaptation10.25225jvb.24063.fullPMIDPMCIDISSN2694-76841573-1189languagecollectionsQ65THHXGdateModified2025-11-07T092025ZkeyAT597N6Blibraryid5891878metacreatorSummaryVau0161eketal.parsedDate2024-05-24numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtVax161ekD.FikarovxE1N.MarkovxE1V.N.HoncO.PacxE1kovxE1L.PorubskxE1B.SomovaV.NovotnxFDJ.MelkesB.KrulovxE1M.ltbgtLipopolysaccharidePretreatmentIncreasestheSensitivityoftheTRPV1ChannelandPromotesanAnti-InflammatoryPhenotypeofCapsaicin-ActivatedMacrophagesltbgt.ltigtJournalofInflammationltigtltbgt2024ltbgtltigt21ltigt117.ltaclass039zp-ItemURL039href039httpsdoi.org10.1186s12950-024-00391-0039gthttpsdoi.org10.1186s12950-024-00391-0ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleLipopolysaccharidepretreatmentincreasesthesensitivityoftheTRPV1channelandpromotesananti-inflammatoryphenotypeofcapsaicin-activatedmacrophagescreatorscreatorTypeauthorfirstNameDaniellastNameVau0161ekcreatorTypeauthorfirstNameNatu00e1lielastNameFikarovu00e1creatorTypeauthorfirstNameVendulaNagylastNameMarkovu00e1creatorTypeauthorfirstNameOndu0159ejlastNameHonccreatorTypeauthorfirstNameLenkalastNamePacu00e1kovu00e1creatorTypeauthorfirstNameBiankalastNamePorubsku00e1creatorTypeauthorfirstNameVeronikalastNameSomovacreatorTypeauthorfirstNameJiu0159u00edlastNameNovotnu00fdcreatorTypeauthorfirstNameBarboralastNameMelkescreatorTypeauthorfirstNameMagdalu00e9nalastNameKrulovu00e1abstractNoteThetransientreceptorpotentialvanilloid1TRPV1iswell-establishedinneuronalfunctionyetitsroleinimmunereactionsremainsenigmatic.Theconflictingdataonitsinflammatoryrolesuggestingbothpro-inflammatoryandanti-inflammatoryeffectsuponTRPV1stimulationinimmunecellsaddscomplexity.TounravelTRPV1immunomodulatorymechanismsweinvestigatedhowtheTRPV1agonistcapsaicininfluenceslipopolysaccharideLPS-inducedpro-inflammatorymacrophagephenotypes.date2024-05-24sectionpartNumberpartTitleDOI10.1186s12950-024-00391-0citationKeyurlhttpsdoi.org10.1186s12950-024-00391-0PMIDPMCIDISSN1476-9255languagecollectionsQ65THHXGdateModified2025-03-07T113828ZkeyBNDTYV99libraryid5891878metacreatorSummaryEliu00e1u0161ovu00e1etal.parsedDate2024-04-18numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtElixE1x161ovxE1P.x160mxEDdB.VejpravovxE1J.LiS.BrivioF.MazurM.RainerD.N.MohideenM.I.H.MorrisR.E.NachtigallP.ltbgtThePreparationRouteandFinalFormofV-MXenesOverridetheEffectoftheOFRatioonTheirMagneticPropertiesltbgt.ltigtJ.Mater.Chem.Cltigtltbgt2024ltbgtltigt12ltigt155431x20135441.ltaclass039zp-DOIURL039href039httpsdoi.org10.1039D4TC00132J039gthttpsdoi.org10.1039D4TC00132Jltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleThepreparationrouteandfinalformofV-MXenesoverridetheeffectoftheOFratioontheirmagneticpropertiescreatorscreatorTypeauthorfirstNamePavlalastNameEliu00e1u0161ovu00e1creatorTypeauthorfirstNameBu0159etislavlastNameu0160mu00eddcreatorTypeauthorfirstNameJanalastNameVejpravovu00e1creatorTypeauthorfirstNameShuolastNameLicreatorTypeauthorfirstNameFedericolastNameBriviocreatorTypeauthorfirstNameMichallastNameMazurcreatorTypeauthorfirstNameDanielN.lastNameRainercreatorTypeauthorfirstNameM.InfasH.lastNameMohideencreatorTypeauthorfirstNameRussellE.lastNameMorriscreatorTypeauthorfirstNamePetrlastNameNachtigallabstractNoteTransitionmetalcarbidesandnitridesMXenesshowahighpotentialforelectrochemicalenergystorageinbatteriesandsupercapacitorsandforelectrocatalysis.Theirexcellentelectronicandmagneticcharacteristicshavebeenhighlightedinseveraltheoreticalstudies.HoweverexperimentalresearchonMXenesisyettoconfirmtheirpredictedpropertiesascandidatesforcontrollablemagnetic2Dmaterials.HerewereportourtheoreticalandexperimentalstudyofV2CTxMXenesTOOHFprovidingkeyinsightsintotheirmagnetism.BasedonourdensityfunctionaltheoryDFTanalysiswepredictedferromagneticFMandantiferromagneticAFMstatesofV2CTxwhicharedeterminedbytheOFratioofsurfacefunctionalgroups.AccordinglywepreparedV2CTxMXenesintheformofmultilayeredpowdersandthinfilmswithdifferentOFratios.NoexperimentalevidenceofFMorAFMpropertieswasfoundinanymaterial.NeverthelesspowdersandfilmswithalmostidenticalchemicalcompositionsintermsofOFratiodisplayeddifferentmagneticpropertieswhereasfilmswithdisparatechemicalcompositionsrevealedasimilarmagneticcharacter.ThereforethepreparationrouteandformofthefinalV2CTxmaterialoverridetheeffectoftheOFratiowhichisoftenoverestimatedintheoreticalstudies.MoreoverthesefindingsunderscoretheimportanceofpreparingMXenematerialstoexperimentallyconfirmtheirtheoreticallypredictedproperties.date2024-04-18sectionpartNumberpartTitleDOI10.1039D4TC00132JcitationKeyurlhttpspubs.rsc.orgencontentarticlelanding2024tcd4tc00132jPMIDPMCIDISSN2050-7534languageencollectionsQ65THHXGdateModified2025-09-06T094713ZkeyUD28CSL2libraryid5891878metacreatorSummaryZumretal.parsedDate2024-03numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtZumrV.NakladalO.RemesJ.ltbgtDeadwood-DwellingBeetlesColeopteraEucnemidaeinaBeechReserveACaseStudyfromtheCzechRepublicltbgt.ltigtForestsltigtltbgt2024ltbgtltigt15ltigt3469.ltaclass039zp-DOIURL039href039httpsdoi.org10.3390f15030469039gthttpsdoi.org10.3390f15030469ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDeadwood-DwellingBeetlesColeopteraEucnemidaeinaBeechReserveACaseStudyfromtheCzechRepubliccreatorscreatorTypeauthorfirstNameVaclavlastNameZumrcreatorTypeauthorfirstNameOtolastNameNakladalcreatorTypeauthorfirstNameJirilastNameRemesabstractNoteThesaproxylicbeetlesdeadwood-dependentbelongtofrequentlystudiedgroupsofforestinsects.Eucnemidaeisarareandpoorlystudiedsaproxylicfamilywithahiddenlifestrictlyrelatedtodeadwood.WestudiedthefamilyEucnemidaeinabeechreserveusing59windowtrapsplacedonstandingdeadwoodsnagsandlyinglogs.Atotalof348specimensineightspecieswererecordedintwoseasons.TheidentifiedspeciesincludedonecriticallyendangeredspeciesCRHyliscarinicepsfiveendangeredspeciesENH.olexaiH.foveicollisIsorhipismelasoidesEucnemiscapucinaandMicrorhaguslepidusonenewspeciesfoundinBohemiaaregionoftheCzechRepublicClypeorhagusclypeatusandonecommonspeciesMelasisbuprestoides.MostspeciespreferredlyinglogsbutE.capucinaandM.buprestoidespreferredsnags.Speciesrichnessq0washigheronlyinglogsthanonsnagsandsimilarlyShannondiversityq1wassignificantlyhigheronlyinglogscomparedtosnags.ThespeciesC.clypeorghagusH.foveicollisH.carinicepsandM.lepidespreferredmoistlyinglogswhileM.buprestoidesandE.capucinapreferreddriersnagswithcavities.TheresultssuggestthatinbeechforestslyinglogsserveasafundamentalhabitatfortheexistenceofEucnemids.Thiscouldbeduetothemorestablemicroclimaticconditionsinsidethelyingdeadwood.FromthisperspectiveourstudymayhelpbetterunderstandthebiologyofhiddenandunderstudiedraresaproxylicEucnemids.dateMAR2024sectionpartNumberpartTitleDOI10.3390f15030469citationKeyurlPMIDPMCIDISSN1999-4907languageEnglishcollectionsQ65THHXGdateModified2025-11-07T092105Zkey3FEMFIJFlibraryid5891878metacreatorSummaryThakuretal.parsedDate2024-03numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtThakurH.AgarwalS.BucekA.HradeckyJ.SehadovaH.MathurV.TogaevU.vandeKampT.HamannE.LiuR.-H.VermaK.S.LiH.-F.Sillam-DussD.EngelM.S.SobotnikJ.ltbgtDefensiveGlandsinStylotermitidaeBlattodeaIsopteraltbgt.ltigtArthropodStruct.Dev.ltigtltbgt2024ltbgtltigt79ltigt101346.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.asd.2024.101346039gthttpsdoi.org10.1016j.asd.2024.101346ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDefensiveglandsinStylotermitidaeBlattodeaIsopteracreatorscreatorTypeauthorfirstNameHimanshulastNameThakurcreatorTypeauthorfirstNameSurbhilastNameAgarwalcreatorTypeauthorfirstNameAleslastNameBucekcreatorTypeauthorfirstNameJaromirlastNameHradeckycreatorTypeauthorfirstNameHanalastNameSehadovacreatorTypeauthorfirstNameVartikalastNameMathurcreatorTypeauthorfirstNameUlugbeklastNameTogaevcreatorTypeauthorfirstNameThomaslastNamevandeKampcreatorTypeauthorfirstNameEliaslastNameHamanncreatorTypeauthorfirstNameRen-HanlastNameLiucreatorTypeauthorfirstNameKuldeepS.lastNameVermacreatorTypeauthorfirstNameHou-FenglastNameLicreatorTypeauthorfirstNameDavidlastNameSillam-DusscreatorTypeauthorfirstNameMichaelS.lastNameEngelcreatorTypeauthorfirstNameJanlastNameSobotnikabstractNoteThelargeabundanceoftermitesispartiallyachievedbytheirdefensiveabilities.StylotermitidaerepresentedbyasingleextantgenusStylotermesisamemberofatermitegroupNeoisopterathatencompasses83oftermitespeciesand94oftermitegeneraandischaracterizedbythepresenceofthefrontalgland.WithinNeoisopteraStylotermitidaerepresentsaspecies-poorsisterlineageofallothergroups.WestudiedthestructureofthefrontallabralandlabialglandsinsoldiersandworkersofStylotermesfaveolusandthecompositionofthefrontalglandsecretioninS.faveolusandStylotermeshalumicus.Weshowthatthefrontalglandisasmallactivesecretoryorganinsoldiersandworkers.Itproducesacocktailofmonoterpenesinsoldiersandsomeofthesemonoterpenesandunidentifiedproteinsinworkers.Thelabralandlabialglandsaredevelopedsimilarlytoothertermitespeciesandcontributetodefensiveactivitieslabralinbothcasteslabialinsoldiersortotheproductionofdigestiveenzymeslabialinworkers.OurresultssupporttheimportanceofthefrontalglandintheevolutionofNeoisoptera.Toxicirritatinganddetectablemonoterpenesplaydefensiveandpheromonalfunctionsandarelikelycriticalnoveltiescontributingtotheecologicalsuccessofthesetermites.c2024ElsevierLtd.Allrightsreserved.dateMAR2024sectionpartNumberpartTitleDOI10.1016j.asd.2024.101346citationKeyurlPMIDPMCIDISSN1467-80391873-5495languageEnglishcollectionsQ65THHXGdateModified2025-11-07T092105Zkey4G4X7TE9libraryid5891878metacreatorSummaryMusteretal.parsedDate2024-03numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMusterC.KorbaJ.BoguschP.HenebergP.x160x165xE1hlavskxFDF.ltbgtAndYetTheyDifferReconsiderationsofDiversitywithinDactylocheliferLatreilliiArachnidaPseudoscorpionesltbgt.ltigtDiversityltigtltbgt2024ltbgtltigt16ltigt3137.ltaclass039zp-DOIURL039href039httpsdoi.org10.3390d16030137039gthttpsdoi.org10.3390d16030137ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleAndYetTheyDifferReconsiderationsofDiversitywithinDactylocheliferlatreilliiArachnidaPseudoscorpionescreatorscreatorTypeauthorfirstNameChristophlastNameMustercreatorTypeauthorfirstNameJanlastNameKorbacreatorTypeauthorfirstNamePetrlastNameBoguschcreatorTypeauthorfirstNamePetrlastNameHenebergcreatorTypeauthorfirstNameFrantiu0161eklastNameu0160u0165u00e1hlavsku00fdabstractNoteMorphologicalstasisisawidespreadcharacteristicofpseudoscorpionssuggestingthatmuchcrypticdiversityremainsunexplored.HerewerevisethepolytypicspeciesDactylocheliferlatreilliiintheframeworkofanintegrativetaxonomicapproachusingDNAbarcodingmultivariateratioanalysisgeometricmorphometryofthemaleforetarsusandgenitalicmorphology.Thepatternofmitochondrialvariationsuggeststhreespecies-levelentitiesincentralEuropewhichwidelyoverlapinmorphospacebutdifferentiateinthestructureofthefemalegenitaliaandbytheirecology.DactylocheliferlatreilliiLeachisahalobiontspeciesoccurringexclusivelyincoastalhabitatsandinPannoniansaltsteppesandD.l.septentrionalisBeiersyn.nov.isajuniorsynonymofthenominatespecies.DactylocheliferdegeeriiC.L.Kochstat.rev.istheoldestavailablenameforaninlandspeciesthathaslongbeenmistakenforthenominotypicalsubspeciesofD.latreillii.Newhabitatinformationsuggestsapreferenceforhighershrubvegetation.DactylocheliferninniiCanestriniistat.rev.isahalophilicMediterraneanspeciesthatextendstothenorthernlimitsofthePannonianbasin.ThedistinctivenessoftheMediterraneanu201cformu201dwasrecognizedbyearlynaturalistsinthe19thcenturybutwasignoredbylaterauthoritiesinthefield.date20243sectionpartNumberpartTitleDOI10.3390d16030137citationKeyurlhttpswww.mdpi.com1424-2818163137PMIDPMCIDISSN1424-2818languageencollectionsQ65THHXGdateModified2025-09-06T094412ZkeyVFWRFG45libraryid5891878metacreatorSummaryFouckovu00e1etal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtFouckovxE1M.UhrovxE1K.KubxE1nkovxE1A.PxE1nekT.CepickaI.ltbgtLightingLanternabovePsalteriomonadidaeUnveilingNovelDiversitywithintheGenusltigtPsalteriomonasltigtDiscobaHeterolobosealtbgt.ltigtEUROPEANJOURNALOFPROTISTOLOGYltigtltbgt2024ltbgtltigt93ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.ejop.2024.126052039gthttpsdoi.org10.1016j.ejop.2024.126052ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleLightinglanternabovePsalteriomonadidaeUnveilingnoveldiversitywithinthegenusiPsalteriomonasiDiscobaHeteroloboseacreatorscreatorTypeauthorfirstNameM.lastNameFouckovu00e1creatorTypeauthorfirstNameK.lastNameUhrovu00e1creatorTypeauthorfirstNameA.lastNameKubu00e1nkovu00e1creatorTypeauthorfirstNameT.lastNamePu00e1nekcreatorTypeauthorfirstNameI.lastNameCepickaabstractNotePsalteriomonadidaeareasmallfamilyofanaerobicfree-livingprotistsbelongingtoHeteroloboseaDiscoba.Wecultured74newstrainsofmostlyamoeboidPsalteriomonadidaeobtainedfrommainlyfreshwaterhabitatsandsequencedtheir18SrRNAgene.Basedonthephylogeneticanalysisandgeneticdistanceswereportmultiplenovelspeciesfourofwhichweformallydescribebasedonthelight-microscopicmorphologyPsalteriomonasminutaP.australisP.fimbriataandP.parva.WealsoexaminedtheultrastructureoftwoPsalteriomonasspeciesusingtransmissionelectronmicroscopy.WetransferSawyeriamarylandensisintothegenusPsalteriomonasandsynonymizeSawyeriawithPsalteriomonas.InadditionwestudiedtheflagellatestageofP.marylandensiscomb.nov.forthefirsttimeusinglightandscanningelectronmicroscopy.date2024sectionpartNumberpartTitleDOI10.1016j.ejop.2024.126052citationKeyurlPMIDPMCIDISSN0932-4739languagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeySU37EXXPlibraryid5891878metacreatorSummaryHobothetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHobothP.SztachoM.HozxE1kP.ltbgtNuclearPatternsofPhosphatidylinositol45-and34-BisphosphateRevealedbySuper-ResolutionMicroscopyDifferbetweentheConsecutiveStagesofRNAPolymeraseIITranscriptionltbgt.ltigtFEBSJOURNALltigtltbgt2024ltbgtltigt291ltigt194240x20134264.ltaclass039zp-DOIURL039href039httpsdoi.org10.1111febs.17136039gthttpsdoi.org10.1111febs.17136ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleNuclearpatternsofphosphatidylinositol45-and34-bisphosphaterevealedbysuper-resolutionmicroscopydifferbetweentheconsecutivestagesofRNApolymeraseIItranscriptioncreatorscreatorTypeauthorfirstNameP.lastNameHobothcreatorTypeauthorfirstNameM.lastNameSztachocreatorTypeauthorfirstNameP.lastNameHozu00e1kabstractNotePhosphatidylinositolphosphatesarepowerfulsignalingmoleculesthatorchestratesignalinganddirectmembranetraffickinginthecytosol.Interestinglyphosphatidylinositolphosphatesalsolocalizewithinthemembrane-lesscompartmentsofthecellnucleuswheretheyparticipateintheregulationofgeneexpression.Neverthelesscurrentmodelsofgeneexpressionwhichincludecondensatesofproteinsandnucleicacidsdonotincludenuclearphosphatidylinositolphosphates.Thisgapispartlyaresultofthemissingdetailedanalysisofthesubnucleardistributionofphosphatidylinositolphosphatesandtheirrelationshipswithgeneexpression.Hereweusedquantitativedual-colordirectstochasticopticalreconstructionmicroscopytoanalyzethenanoscaleco-patterningbetweenRNApolymeraseIItranscriptioninitiationandelongationmarkerswithrespecttophosphatidylinositol45-or34-bisphosphateinthenucleoplasmandnuclearspecklesandcompareditwithrandomizeddataandcellswithinhibitedtranscription.Wefoundspecificco-patterningofthetranscriptioninitiationmarkerP-S5withphosphatidylinositol45-bisphosphateinthenucleoplasmandwithphosphatidylinositol34-bisphosphateattheperipheryofnuclearspeckles.WeshowedthespecificaccumulationofthetranscriptionelongationmarkerPS-2andofnascentRNAintheproximityofphosphatidylinositol34-bisphosphateassociatedwithnuclearspeckles.TakentogetherthisshowsthatthedistinctspatialassociationsbetweentheconsecutivestagesofRNApolymeraseIItranscriptionandnuclearphosphatidylinositolphosphatesexhibitspecificitywithinthegeneexpressioncompartments.ThusinanalogytothecellularmembraneswherephospholipidcompositionorchestratessignalingpathwaysanddirectsmembranetraffickingweproposeamodelinwhichthephospholipididentityofgeneexpressioncompartmentsorchestratesRNApolymeraseIItranscription.date2024sectionpartNumberpartTitleDOI10.1111febs.17136citationKeyurlPMIDPMCIDISSN1742-464XlanguagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeyNN4YVNJ8libraryid5891878metacreatorSummarySoukupetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtSoukupJ.ZelenxE1M.WeiszF.KostelanskxE1M.NohynkovxE1E.TumovxE1P.ltbgtImagingGiardiaIntestinalisCellularOrganisationUsingExpansionMicroscopyRevealsAtypicalCentrinLocalisationltbgt.ltigtEXPERIMENTALPARASITOLOGYltigtltbgt2024ltbgtltigt266ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.exppara.2024.108831039gthttpsdoi.org10.1016j.exppara.2024.108831ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleImagingGiardiaintestinaliscellularorganisationusingexpansionmicroscopyrevealsatypicalcentrinlocalisationcreatorscreatorTypeauthorfirstNameJ.lastNameSoukupcreatorTypeauthorfirstNameM.lastNameZelenu00e1creatorTypeauthorfirstNameF.lastNameWeiszcreatorTypeauthorfirstNameM.lastNameKostelansku00e1creatorTypeauthorfirstNameE.lastNameNohynkovu00e1creatorTypeauthorfirstNameP.lastNameTumovu00e1abstractNoteAdvancedimagingofmicroorganismsincludingprotistsischallengingduetotheirsmallsize.Specimenexpansionpriortoimagingisthusbeneficialtoincreaseresolutionandcellulardetails.Herewepresentasamplepreparationworkflowforimprovedobservationsofthesingle-celledeukaryoticpathogenGiardiaintestinalisExcavataMetamonada.Thebinucleatedtrophozoitescolonizethesmallintestineofhumansandanimalsandcauseadiarrhoealdisease.Theirremarkablemorphologyincludestwonucleiandapronouncedmicrotubularcytoskeletonenablingcellmotilityattachmentandproliferation.Byuseofexpansionandconfocalmicroscopyweresolvedinagreatdetailsubcellularstructuresandorganellesoftheparasitecell.TheacquiredspatialresolutionenablednovelobservationsofcentrinlocalizationatGiardiabasalbodies.Interestinglynon-luminalcentrinlocalizationbetweentheGiardiabasalbodieswasobservedwhichisanatypicaleukaryoticarrangement.Ourprotocolincludesantibodystainingandcanbeusedforthelocalizationofepitope-taggedproteinsaswellasfordifferentialorganellelabellingbyaminoreactiveesters.Thisfastandsimpletechniqueissuitableforroutineusewithoutasuperresolutionmicroscopyequipment.date2024sectionpartNumberpartTitleDOI10.1016j.exppara.2024.108831citationKeyurlPMIDPMCIDISSN0014-4894languagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeyHYT3FIG4libraryid5891878metacreatorSummaryAlietal.parsedDate2024numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtAliE.x10CervenkovxE1L.PxE1lekR.AmbrozkiewiczF.Hox161ekP.DaumO.Lix161kaV.HemminkiK.TrailinA.ltbgtPrognosticRoleofMacrophagesandMastCellsintheMicroenvironmentofHepatocellularCarcinomaafterResectionltbgt.ltigtBMCCancerltigtltbgt2024ltbgtltigt24ltigt1142.ltaclass039zp-ItemURL039href039httpsdoi.org10.1186s12885-024-11904-8039gthttpsdoi.org10.1186s12885-024-11904-8ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitlePrognosticroleofmacrophagesandmastcellsinthemicroenvironmentofhepatocellularcarcinomaafterresectioncreatorscreatorTypeauthorfirstNameEsraalastNameAlicreatorTypeauthorfirstNameLenkalastNameu010cervenkovu00e1creatorTypeauthorfirstNameRichardlastNamePu00e1lekcreatorTypeauthorfirstNameFiliplastNameAmbrozkiewiczcreatorTypeauthorfirstNamePetrlastNameHou0161ekcreatorTypeauthorfirstNameOndrejlastNameDaumcreatorTypeauthorfirstNameVu00e1clavlastNameLiu0161kacreatorTypeauthorfirstNameKarilastNameHemminkicreatorTypeauthorfirstNameAndriylastNameTrailinabstractNoteTheprognosticsignificanceofmastcellsanddifferentphenotypesofmacrophagesinthemicroenvironmentofhepatocellularcarcinomaHCCfollowingresectionisunclear.WeaimedinthisstudytoassessthelocaldistributionofinfiltratingmacrophagesandmastcellsofspecificphenotypesintissuesofHCCandtoevaluatetheirprognosticvaluesforsurvivalofpost-surgicalpatients.date2024sectionpartNumberpartTitleDOI10.1186s12885-024-11904-8citationKeyurlhttpsdoi.org10.1186s12885-024-11904-8PMIDPMCIDISSN1471-2407languagecollectionsQ65THHXGdateModified2025-03-19T115835Zkey5I96ZSJFlibraryid5891878metacreatorSummaryBourlandetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtBourlandW.PomahacO.CepickaI.ltbgtRedescriptionandMolecularPhylogenyoftheFreshwaterMetopidCastulaStrelkowiJankowski1964fromtheCzechRepublicandSynonymizationofPileometopuswithCastulaltbgt.ltigtPROTISTltigtltbgt2024ltbgtltigt175ltigt3.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.protis.2024.126034039gthttpsdoi.org10.1016j.protis.2024.126034ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleRedescriptionandmolecularphylogenyofthefreshwatermetopidCastulastrelkowiJankowski1964fromtheCzechRepublicandsynonymizationofPileometopuswithCastulacreatorscreatorTypeauthorfirstNameW.lastNameBourlandcreatorTypeauthorfirstNameO.lastNamePomahaccreatorTypeauthorfirstNameI.lastNameCepickaabstractNoteTherelationshipsofthemainlyfreelivingobligatelyanaerobicciliatedprotistsbelongingtoorderMetopidacontinuetobeclarifiedandnowcomprisethreefamiliesMetopidaeTropidoatractidaeandApometopidae.Themostspecies-richgenusoftheMetopidaeMetopushasundergoneconsiderablesubdivisionintonewgenerainrecentyearsasmoretaxaarecharacterizedbymodernmorphologicandmolecularmethods.ThegenusCastulawasestablishedtoaccommodatesetae-bearingspeciespreviouslyassignedtoMetopusC.setosaandC.fuscaandonenewspeciesC.flexibilis.AnothernewspeciesC.specialishasbeenaddedsince.HereweredescribeanotherspeciespreviouslyincludedinMetopususingmorphologicandmolecularmethodsandtransferittoCastulaasC.strelkowin.comb.originalcombinationMetopusstrelkowi.WealsoreassessthemonotypicgenusPileometopuswhichnestswithinthestronglysupportedCastulacladein18SrRNAgenetreesandconcludethatitrepresentsamorphologicallydivergentspeciesofCastula.date2024sectionpartNumberpartTitleDOI10.1016j.protis.2024.126034citationKeyurlPMIDPMCIDISSN1434-4610languagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeyTYYYYK6Ilibraryid5891878metacreatorSummarySaboetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtSaboJ.ZdimalovaM.D.SlaterP.G.DostalV.HerynekS.LibusovaL.LoweryL.A.BraunM.LanskyZ.ltbgtCKAP5EnablesFormationofPersistentActinBundlesTemplatedbyDynamicallyInstableMicrotubulesltbgt.ltigtCURRENTBIOLOGYltigtltbgt2024ltbgtltigt34ltigt2.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.cub.2023.11.031039gthttpsdoi.org10.1016j.cub.2023.11.031ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleCKAP5enablesformationofpersistentactinbundlestemplatedbydynamicallyinstablemicrotubulescreatorscreatorTypeauthorfirstNameJ.lastNameSabocreatorTypeauthorfirstNameM.D.lastNameZdimalovacreatorTypeauthorfirstNameP.G.lastNameSlatercreatorTypeauthorfirstNameV.lastNameDostalcreatorTypeauthorfirstNameS.lastNameHerynekcreatorTypeauthorfirstNameL.lastNameLibusovacreatorTypeauthorfirstNameL.A.lastNameLowerycreatorTypeauthorfirstNameM.lastNameBrauncreatorTypeauthorfirstNameZ.lastNameLanskyabstractNoteCytoskeletalrearrangementsandcrosstalkbetweenmicrotubulesandactinfilamentsarevitalforlivingorganisms.RecentlyanabundantlypresentmicrotubulepolymeraseCKAP5XMAP215homologhasbeenreportedtoplayaroleinmediatingcrosstalkbetweenmicrotubulesandactinfilamentsintheneuronalgrowthcones.Howeverthemolecularmechanismofthisprocessisunknown.HerewedemonstrateinareconstitutedsystemthatCKAP5enablestheformationofpersistentactinbundlestemplatedbydynamicallyinstablemicrotubules.WeexplainthetemplatingbythedifferenceinCKAP5bindingtomicrotubulesandactinfilaments.BindingtothemicrotubulelatticewithhigheraffinityCKAP5enablestheformationofactinbundlesexclusivelyonthemicrotubulelatticeatCKAP5concentrationsinsufficienttosupportanyactinbundlingintheabsenceofmicrotubules.Strikinglywhenthemicrotubulesdepolymerizeactinbundlesprevailatthepositionspredeterminedbythemicrotubules.WeproposethatthelocalabundanceofavailableCKAP5-bindingsitesinactinbundlesallowstheretentionofCKAP5resultinginpersistingactinbundles.InlinewithourobservationswefoundthatreducingCKAP5levelsinvivoresultsinadecreaseinactin-microtubuleco-localizationingrowthconesandspecificallydecreasesactinintensityatmicrotubuleplusends.Thisreadilysuggestsamechanismexplaininghowexploratorymicrotubulessetthepositionsofactinbundlesforexampleincytoskeleton-richneuronalgrowthcones.date2024sectionpartNumberpartTitleDOI10.1016j.cub.2023.11.031citationKeyurlPMIDPMCIDISSN0960-9822languagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeyALHIE4B4libraryid5891878metacreatorSummaryFleischhacker-Daffertetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtFleischhacker-DaffertC.ZerobinA.HummelF.SlaninovaE.KroupovaZ.ObrucaS.MrazovaK.HrubanovaK.KrzyzanekV.NebesarovaJ.LudwigK.FritzI.ltbgtAComparisonoftheEffectsofContinuousIlluminationandDayNightRegimesonPHBAccumulationinltigtSynechocystisltigtCellsltbgt.ltigtLIFE-BASELltigtltbgt2024ltbgtltigt14ltigt7.ltaclass039zp-DOIURL039href039httpsdoi.org10.3390life14070907039gthttpsdoi.org10.3390life14070907ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleAComparisonoftheEffectsofContinuousIlluminationandDayNightRegimesonPHBAccumulationiniSynechocystisiCellscreatorscreatorTypeauthorfirstNameC.lastNameFleischhacker-DaffertcreatorTypeauthorfirstNameA.lastNameZerobincreatorTypeauthorfirstNameF.lastNameHummelcreatorTypeauthorfirstNameE.lastNameSlaninovacreatorTypeauthorfirstNameZ.lastNameKroupovacreatorTypeauthorfirstNameS.lastNameObrucacreatorTypeauthorfirstNameK.lastNameMrazovacreatorTypeauthorfirstNameK.lastNameHrubanovacreatorTypeauthorfirstNameV.lastNameKrzyzanekcreatorTypeauthorfirstNameJ.lastNameNebesarovacreatorTypeauthorfirstNameK.lastNameLudwigcreatorTypeauthorfirstNameI.lastNameFritzabstractNotePoly3-hydroxybutyratePHBisabiobasedandbiodegradablepolymerwithpropertiescomparabletopolypropyleneandthereforehasthepotentialtoreplaceconventionalplastics.PHBisintracellularlyaccumulatedbyprokaryoticorganisms.ForthecellsPHBfunctionsmanlyascarbonandenergysourcebutallpossiblefunctionsofPHBarestillnotknown.SynechocystiscyanobacteriaaccumulatesPHBusinglightasenergyandCO2ascarbonsource.ThemaintriggerforPHBaccumulationincyanobacteriaisnitrogenandphosphorousdepletionwithsimultaneoussurplusofcarbonandenergy.FortheabovereasonsobtainingknowledgeaboutexternalfactorsinfluencingPHBaccumulationisofhighestinterest.Thisstudycomparestheeffectofcontinuouslightexposureanddaynight168hcyclesonselectedphysiologyparametersofthreeSynechocystisstrains.WeshowthatcontinuousilluminationatmoderatelightintensitiesleadstoanincreasedPHBaccumulationinSynechocystissalinaCCALA192max.14.2CDW-celldryweightcomparedtodaynightcycles3.7CDW.InadditiontoPHBcontentglycogenandcellsizeincreasedwhilecelldensityandcellviabilitydecreased.TheresultsoffernewapproachesforfurtherstudiestogaindeeperinsightsintotheroleofPHBincyanobacteriatoobtainbioplasticsinamoresustainableandenvironmentallyfriendlyway.date2024sectionpartNumberpartTitleDOI10.3390life14070907citationKeyurlPMIDPMCIDISSN2075-1729languagecollectionsQ65THHXGdateModified2025-03-19T115834ZkeyFXX8QAIQlibraryid5891878metacreatorSummaryAlietal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtAliE.CervenkovxE1L.PxE1lekR.AmbrozkiewiczF.PavlovS.YeW.J.HosekP.DaumO.LiskaV.HemminkiK.TrailinA.ltbgtMastCellsintheMicroenvironmentofHepatocellularCarcinomaConferFavorablePrognosisARetrospectiveStudyUsingQuPathImageAnalysisSoftwareltbgt.ltigtJOVE-JOURNALOFVISUALIZEDEXPERIMENTSltigtltbgt2024ltbgtNo.206.ltaclass039zp-DOIURL039href039httpsdoi.org10.379166743039gthttpsdoi.org10.379166743ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMastCellsintheMicroenvironmentofHepatocellularCarcinomaConferFavorablePrognosisARetrospectiveStudyusingQuPathImageAnalysisSoftwarecreatorscreatorTypeauthorfirstNameE.lastNameAlicreatorTypeauthorfirstNameL.lastNameCervenkovu00e1creatorTypeauthorfirstNameR.lastNamePu00e1lekcreatorTypeauthorfirstNameF.lastNameAmbrozkiewiczcreatorTypeauthorfirstNameS.lastNamePavlovcreatorTypeauthorfirstNameW.J.lastNameYecreatorTypeauthorfirstNameP.lastNameHosekcreatorTypeauthorfirstNameO.lastNameDaumcreatorTypeauthorfirstNameV.lastNameLiskacreatorTypeauthorfirstNameK.lastNameHemminkicreatorTypeauthorfirstNameA.lastNameTrailinabstractNoteTheinsightsprovidedbyin-situdetectionofimmunecellswithinhepatocellularcarcinomaHCCmightpresentinformationonpatientoutcomes.Studiesinvestigatingtheexpressionandlocalizationofimmunecellswithintumortissuesareassociatedwithseveralchallengesincludingalackofpreciseannotationfortumorregionsandrandomselectionofmicroscopicfieldsofview.QuPathisanopen-sourceuser-friendlysoftwarethatcouldmeetthegrowingneedfordigitalpathologyinwholeslideimageWSIanalysis.TheinfiltrationofHCCandadjacenttissuesbyCD1aimmaturedendriticcellsiDCsCD117mastcellsandNKp46naturalkillercellsNKscellswasassessedimmunohistochemicallyinrepresentativespecimensof67patientswithHCCwhounderwentcurativeresection.TheareafractionAFofpositivelystainedcellswasassessedautomaticallyinWSIsusingQuPathinthetumorcenterTCinnermarginIMoutermarginOMandperitumorPTarea.TheprognosticsignificanceofimmunecellswasevaluatedfortimetorecurrenceTTRdisease-freesurvivalDFSandoverallsurvivalOS.TheAFofmastcellswassignificantlygreaterthantheAFofNKsandtheAFofiDCswassignificantlylowercomparedtoNKsineachregionofinterest.HighAFsofmastcellsintheIMandPTareaswereassociatedwithlongerDFS.InadditionhighAFofmastcellsinIMwasassociatedwithlongerOS.Computer-assistedanalysisusingthissoftwareisasuitabletoolforobtainingprognosticinformationfortumor-infiltratingimmunecellsiDCsmastcellsandNKsindifferentregionsofHCCafterresection.MastcellsdisplayedthegreatestAFinallregionsofinterestROIs.MastcellsintheperitumorregionandIMshowedapositiveprognosticsignificance.date2024sectionpartNumberpartTitleDOI10.379166743citationKeyurlPMIDPMCIDISSN1940-087XlanguagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeyNJ4N97LYlibraryid5891878metacreatorSummaryZhangetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtZhangJ.YueQ.D.ShammaE.AbdiS.PetrovO.CejkaJ.MintovaS.OpanasenkoM.ShamzhyM.ltbgtBalancingGeDe-IntercalationandSiRe-InsertionRatesStabilizesHydrolyticallyLabileGermanosilicateZeolitesltbgt.ltigtJOURNALOFMATERIALSCHEMISTRYAltigtltbgt2024ltbgtltigt12ltigt4531195x201331203.ltaclass039zp-DOIURL039href039httpsdoi.org10.1039d4ta05539j039gthttpsdoi.org10.1039d4ta05539jltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleBalancingGede-intercalationandSire-insertionratesstabilizeshydrolyticallylabilegermanosilicatezeolitescreatorscreatorTypeauthorfirstNameJ.lastNameZhangcreatorTypeauthorfirstNameQ.D.lastNameYuecreatorTypeauthorfirstNameE.lastNameShammacreatorTypeauthorfirstNameS.lastNameAbdicreatorTypeauthorfirstNameO.lastNamePetrovcreatorTypeauthorfirstNameJ.lastNameCejkacreatorTypeauthorfirstNameS.lastNameMintovacreatorTypeauthorfirstNameM.lastNameOpanasenkocreatorTypeauthorfirstNameM.lastNameShamzhyabstractNoteGermanosilicatezeolitesareattractiveadsorbentsandcatalyststhankstotheirdiversestructuresandversatiletexturalproperties.Howeverhydrolyticinstabilityofsuchzeolitesevenunderambientconditionsrestrictstheirpracticalapplications.InthisstudywereportdynamicchangesinthestateofthezeoliteframeworkatomsduetoGede-intercalationandSire-insertioninaqueousmediumandproposeastrategyforstabilizingzeoliteswithplanarandorthogonallocationsofGe-richdomainsbymanagingbothprocesses.AdjustingtheacidityortemperatureoftheaqueousenvironmentenabledGeandSiatomstoreachbalancedmobilityallowingGeatomstobeleachedandSiatomstobeinsertedintothereleasedpositionstherebystabilizingthezeoliteframework.ThedevelopedapproachoffersapracticalandcontrollablemethodforstructuralstabilizationofanylabilegermanosilicatematerialwithpotentialapplicationsincatalysisasdemonstratedaftertheincorporationofAl-associatedacidcenters.date2024sectionpartNumberpartTitleDOI10.1039d4ta05539jcitationKeyurlPMIDPMCIDISSN2050-7488languagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeyK9VZZ7IIlibraryid5891878metacreatorSummarySchrecengostetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtSchrecengostA.RotterovxE1J.PolxE1kovxE1K.CepickaI.BeinartR.A.ltbgtDivergentMarineAnaerobicCiliatesHarborCloselyRelatedMethanocorpusculumEndosymbiontsltbgt.ltigtISMEJOURNALltigtltbgt2024ltbgtltigt18ltigt1.ltaclass039zp-DOIURL039href039httpsdoi.org10.1093ismejowrae125039gthttpsdoi.org10.1093ismejowrae125ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDivergentmarineanaerobicciliatesharborcloselyrelatedMethanocorpusculumendosymbiontscreatorscreatorTypeauthorfirstNameA.lastNameSchrecengostcreatorTypeauthorfirstNameJ.lastNameRotterovu00e1creatorTypeauthorfirstNameK.lastNamePolu00e1kovu00e1creatorTypeauthorfirstNameI.lastNameCepickacreatorTypeauthorfirstNameR.A.lastNameBeinartabstractNoteCiliatesareadiversegroupofprotistsknownfortheirabilitytoestablishvariouspartnershipsandthriveinawidevarietyofoxygen-depletedenvironments.Mostanaerobicciliatesharbormethanogensoneofthefewknownarchaealivingintracellularly.Thesemethanogensincreasethemetabolicefficiencyofhostfermentationviasyntrophicuseofhostend-productinmethanogenesis.Despitetheubiquityofthesesymbiosesinanoxichabitatspatternsofsymbiontspecificityandfidelityarenotwellknown.WesurveyedtwounrelatedcommonlyfoundgroupsofanaerobicciliatesthePlagiopyleaandMetopidaisolatedfromanoxicmarinesediments.Wesequencedhost18SrRNAandsymbiont16SrRNAmarkergenesaswellasthesymbiontinternaltranscribedspacerregionfromourculturedciliatestoidentifyhostsandtheirassociatedmethanogenicsymbionts.Wefoundthatmarineciliatesfrombothoftheseco-occurringdivergentgroupsharborcloselyrelatedyetdistinctintracellulararchaeawithintheMethanocorpusculumgenus.Thesymbiontsappeartobestableatthehostspecieslevelbutathighertaxonomiclevelsthereisevidencethatsymbiontreplacementshaveoccurred.Gaininginsightintothisuniqueassociationwilldeepenourunderstandingofthecomplextransmissionmodesofmarinemicrobialsymbiontsandthemutualisticmicrobialinteractionsoccurringacrossdomainsoflife.date2024sectionpartNumberpartTitleDOI10.1093ismejowrae125citationKeyurlPMIDPMCIDISSN1751-7362languagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeyD8JMNHR4libraryid5891878metacreatorSummaryRoucovu00e1etal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtRoucovxE1K.VopxE1lenskyV.MasekT.LlanoE.D.ProvaznxEDkJ.LandryJ.J.M.AzevedoN.EhlerE.BenesV.PospxEDsekM.ltbgtLossofADAR1ProteinInducesChangesinSmallRNALandscapeinHepatocytesltbgt.ltigtRNAltigtltbgt2024ltbgtltigt30ltigt91164x20131183.ltaclass039zp-DOIURL039href039httpsdoi.org10.1261rna.080097.124039gthttpsdoi.org10.1261rna.080097.124ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleLossofADAR1proteininduceschangesinsmallRNAlandscapeinhepatocytescreatorscreatorTypeauthorfirstNameK.lastNameRoucovu00e1creatorTypeauthorfirstNameV.lastNameVopu00e1lenskycreatorTypeauthorfirstNameT.lastNameMasekcreatorTypeauthorfirstNameE.DellastNameLlanocreatorTypeauthorfirstNameJ.lastNameProvaznu00edkcreatorTypeauthorfirstNameJ.J.M.lastNameLandrycreatorTypeauthorfirstNameN.lastNameAzevedocreatorTypeauthorfirstNameE.lastNameEhlercreatorTypeauthorfirstNameV.lastNameBenescreatorTypeauthorfirstNameM.lastNamePospu00edsekabstractNoteInrecentyearsnumerousevidencehasbeenaccumulatedabouttheextentofA-to-IeditinginhumanRNAsandthekeyroleADAR1playsinthecellulareditingmachinery.IthasbeenshownthatA-to-Ieditingoccurrenceandfrequencyaretissue-specificandessentialforsometissuedevelopmentsuchastheliver.TostudytheeffectofADAR1functioninhepatocyteswehavecreatedHuh7.5ADAR1KOcelllines.UponIFNtreatmenttheHuh7.5ADAR1KOcellsshowrapidarrestofgrowthandtranslationfromwhichtheydonotrecover.WeanalyzedtranslatomechangesbyusingamethodbasedonsequencingofseparatepolysomeprofileRNAfractions.WefoundsignificantchangesinthetranscriptomeandtranslatomeoftheHuh7.5ADAR1KOcells.ThemostprominentchangesincludenegativelyaffectedtranscriptionbyRNApolymeraseIIIandthederegulationofsnoRNAandYRNAlevels.FurthermoreweobservedthatADAR1KOpolysomesareenrichedinmRNAscodingforproteinspivotalinawiderangeofbiologicalprocessessuchasRNAlocalizationandRNAprocessingwhereastheunboundfractionisenrichedmainlyinmRNAscodingforribosomalproteinsandtranslationalfactors.ThisindicatesthatADAR1playsamorerelevantroleinsmallRNAmetabolismandribosomebiogenesis.date2024sectionpartNumberpartTitleDOI10.1261rna.080097.124citationKeyurlPMIDPMCIDISSN1355-8382languagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeyH9H3VTG7libraryid5891878metacreatorSummaryMaseketal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMasekJ.FilipovicI.HulN.V.BelicovaL.JirouskovxE1M.OliveiraD.FrontinoA.M.HankeovaS.HeJ.Y.TurettiF.IqbalA.CervenkaI.SarnovaL.VerbovenE.BrabecT.BjoerkstrxF6mN.K.GregorM.DobesJ.AnderssonE.R.ltbgtJag1InsufficiencyAltersLiverFibrosisviaTCellandHepatocyteDifferentiationDefectsltbgt.ltigtEMBOMOLECULARMEDICINEltigtltbgt2024ltbgtltigt16ltigt112946x20132975.ltaclass039zp-DOIURL039href039httpsdoi.org10.1038s44321-024-00145-8039gthttpsdoi.org10.1038s44321-024-00145-8ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleJag1insufficiencyaltersliverfibrosisviaTcellandhepatocytedifferentiationdefectscreatorscreatorTypeauthorfirstNameJ.lastNameMasekcreatorTypeauthorfirstNameI.lastNameFilipoviccreatorTypeauthorfirstNameN.VanlastNameHulcreatorTypeauthorfirstNameL.lastNameBelicovacreatorTypeauthorfirstNameM.lastNameJirouskovu00e1creatorTypeauthorfirstNameD.lastNameOliveiracreatorTypeauthorfirstNameA.M.lastNameFrontinocreatorTypeauthorfirstNameS.lastNameHankeovacreatorTypeauthorfirstNameJ.Y.lastNameHecreatorTypeauthorfirstNameF.lastNameTuretticreatorTypeauthorfirstNameA.lastNameIqbalcreatorTypeauthorfirstNameI.lastNameCervenkacreatorTypeauthorfirstNameL.lastNameSarnovacreatorTypeauthorfirstNameE.lastNameVerbovencreatorTypeauthorfirstNameT.lastNameBrabeccreatorTypeauthorfirstNameN.K.lastNameBjoerkstru00f6mcreatorTypeauthorfirstNameM.lastNameGregorcreatorTypeauthorfirstNameJ.lastNameDobescreatorTypeauthorfirstNameE.R.lastNameAnderssonabstractNoteFibrosiscontributestotissuerepairbutexcessivefibrosisdisruptsorganfunction.AlagillesyndromeALGScausedbymutationsinJAGGED1resultsinliverdiseaseandcharacteristicfibrosis.HereweshowthatJag1NdrNdrmiceamodelforALGSrecapitulateALGS-likefibrosis.Single-cellRNA-seqandmulti-colorflowcytometryoftheliverrevealedimmaturehepatocytesandparadoxicallylowintrahepaticTcellinfiltrationdespitecholestasisinJag1NdrNdrmice.ThymicandsplenicregulatoryTcellsTregswereenrichedandJag1NdrNdrlymphocyteimmuneandfibroticcapacitywastestedwithadoptivetransferintoRag1--micechallengedwithdextransulfatesodiumDSSorbileductligationBDL.TransplantedJag1NdrNdrlymphocyteswerelessinflammatorywithfeweractivatedTcellsthanJag1lymphocytesinresponsetoDSS.CholestasisinducedbyBDLinRag1--micewithJag1NdrNdrlymphocytesresultedinperiportalTregaccumulationandthree-foldlessperiportalfibrosisthaninRag1--micewithJag1lymphocytes.FinallytheJag1NdrNdrhepatocyteexpressionprofileandTregoverrepresentationwerecorroboratedinpatients039liversamples.Jag1-dependenthepaticandimmunedefectsthusinteracttodeterminethefibroticprocessinALGS.DespiteseverecholestaticliverdiseaseduetobileductpaucityintrahepaticfibrosisinAlagillesyndromeALGSdiffersfromothercholestaticliverdiseases.ThewaycellpopulationsareaffectedbyALGSandinteracttoinfluencediseaseprogressionwasinvestigatedinanALGSmousemodel.IntrahepaticALGS-likepericellularfibrosisisrecapitulatedbymice.Single-celltranscriptomicsandflowcytometryidentifieddysregulationofmaturinghepatocytesandTcellsduringfibrosisonsetandpropagation.andALGShepatocytesexpressahepatoblast-likesignaturesuggestingdisruptedhepatocytematurationandcompromisedactivation.RegulatoryTcellsareenrichedinmiceandcanlimitperiportalfibrosisasdemonstratedbycelltransplantationsintoimmunodeficientmicefollowedbysurgicallyinducedcholestasisDespiteseverecholestaticliverdiseaseduetobileductpaucityintrahepaticfibrosisinAlagillesyndromeALGSdiffersfromothercholestaticliverdiseases.ThewaycellpopulationsareaffectedbyALGSandinteracttoinfluencediseaseprogressionwasinvestigatedinanALGSmousemodel.date2024sectionpartNumberpartTitleDOI10.1038s44321-024-00145-8citationKeyurlPMIDPMCIDISSN1757-4676languagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeyXPVVLBEAlibraryid5891878metacreatorSummaryHuberetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHuberB.A.MengG.L.DederichsT.M.MichalikP.FormanM.KrxE1lJ.ltbgtCastawaysTheLeewardAntillesEndemicSpiderGenusltigtPapiamentaltigtAraneaePholcidaeltbgt.ltigtINVERTEBRATESYSTEMATICSltigtltbgt2024ltbgtltigt38ltigt2.ltaclass039zp-DOIURL039href039httpsdoi.org10.1071IS23052039gthttpsdoi.org10.1071IS23052ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleCastawaystheLeewardAntillesendemicspidergenusiPapiamentaiAraneaePholcidaecreatorscreatorTypeauthorfirstNameB.A.lastNameHubercreatorTypeauthorfirstNameG.L.lastNameMengcreatorTypeauthorfirstNameT.M.lastNameDederichscreatorTypeauthorfirstNameP.lastNameMichalikcreatorTypeauthorfirstNameM.lastNameFormancreatorTypeauthorfirstNameJ.lastNameKru00e1labstractNoteNinetinaeisagroupofsmalltotinyshort-leggedspiderslargelyrestrictedtoaridhabitats.Amongdaddy-long-legsspidersPholcidaethisisbyfartheleastdiversesubfamilybutthismaypartlybearesultofinadequatecollectingpoorrepresentationincollectionsorscientificneglect.WebuildonalargerecentcollectionoftheninetinegenusPapiamentaHuber2000fromtheLeewardAntillesandusecytochromeoxidase1COIsequencesextensivescanningelectronmicroscopydatatransmissionelectronmicroscopydataandkaryotypingtoanalysethisgeographicallyisolatedandpoorlyknownislandgenus.COIsequencessupportthesplitbetweenthetwomorphologicallydistinctspeciesonCuraampccedilaobutgeneticdistancesbetweenthesearesurprisinglylow7.4-9.8mean8.6.ThetypespeciesP.leviiGertsch1982mayincludemorethanonespeciesbutCOIandmorphologysuggestconflictingcladelimits.AthirdspeciesP.bonayHubersp.nov.isnewlydescribedfromBonaire.OurdataonspermultrastructureandkaryologyarepuzzlingasthesesuggestdifferentphylogeneticaffinitiesofPapiamentatoothergenera.MalestransferspermasindividualspermcleistospermagreeingwiththeputativeclosestrelativesassuggestedbymoleculardatatheNorthAmericangeneraPholcophoraandTolteca.ThesexchromosomesystemX1X2X3YofP.leviihoweverisasintheSouthAmericanNinetinaegeneraGertschiolaandNerudiabutdifferentfromtheputativeclosestrelatives.date2024sectionpartNumberpartTitleDOI10.1071IS23052citationKeyurlPMIDPMCIDISSN1445-5226languagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeyV6FD49UJlibraryid5891878metacreatorSummaryHuebbersetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHuebbersJ.W.CaldarescuG.A.KubxE1tovxE1Z.SabolP.LevecqueS.C.J.KuhnH.KulichI.ReinstxE4dlerA.BxFCttgenK.Manga-RoblesA.MxE9lidaH.PaulyM.PanstrugaR.ZxE1rskyV.ltbgtInterplayofEXO70andMLOProteinsModulatesTrichomeCellWallCompositionandSusceptibilitytoPowderyMildewltbgt.ltigtPLANTCELLltigtltbgt2024ltbgt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1093plcellkoad319039gthttpsdoi.org10.1093plcellkoad319ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleInterplayofEXO70andMLOproteinsmodulatestrichomecellwallcompositionandsusceptibilitytopowderymildewcreatorscreatorTypeauthorfirstNameJ.W.lastNameHuebberscreatorTypeauthorfirstNameG.A.lastNameCaldarescucreatorTypeauthorfirstNameZ.lastNameKubu00e1tovu00e1creatorTypeauthorfirstNameP.lastNameSabolcreatorTypeauthorfirstNameS.C.J.lastNameLevecquecreatorTypeauthorfirstNameH.lastNameKuhncreatorTypeauthorfirstNameI.lastNameKulichcreatorTypeauthorfirstNameA.lastNameReinstu00e4dlercreatorTypeauthorfirstNameK.lastNameBu00fcttgencreatorTypeauthorfirstNameA.lastNameManga-RoblescreatorTypeauthorfirstNameH.lastNameMu00e9lidacreatorTypeauthorfirstNameM.lastNamePaulycreatorTypeauthorfirstNameR.lastNamePanstrugacreatorTypeauthorfirstNameV.lastNameZu00e1rskyabstractNoteExocystcomponentof70-kDaEXO70proteinsareconstituentsoftheexocystcompleximplicatedinvesicletetheringduringexocytosis.MILDEWRESISTANCELOCUSOMLOproteinsareplant-specificcalciumchannelsandsomeMLOisoformsenablefungalpowderymildewpathogenesis.WeheredetectedanunexpectedphenotypicoverlapofArabidopsisthalianaexo70H4andmlo2mlo6mlo12triplemutantplantsregardingthebiogenesisofleaftrichomesecondarycellwalls.BiochemicalandFouriertransforminfraredspectroscopicanalysescorroborateddeficienciesinthecompositionoftrichomecellwallsinthesemutants.Transgeniclinesexpressingfluorophore-taggedEXO70H4andMLOexhibitedextensivecolocalizationoftheseproteins.FurthermoremCherry-EXO70H4mislocalizedintrichomesofthemlotriplemutantandviceversaMLO6-GFPmislocalizedintrichomesoftheexo70H4mutant.ExpressionofGFP-markedPMR4callosesynthaseaknowncargoofEXO70H4-dependentexocytosisrevealedreducedcellwalldeliveryofGFP-PMR4intrichomesofmlotriplemutantplants.Invivoprotein-proteininteractionassaysinplantandyeastcellsuncoveredisoform-preferentialinteractionsbetweenEXO70.2subfamilymembersandMLOproteins.Finallyexo70H4andmlo6mutantswhencombinedshowedsynergisticallyenhancedresistancetopowderymildewattack.Takentogetherourdatapointtoanisoform-specificinterplayofEXO70andMLOproteinsinthemodulationoftrichomecellwallbiogenesisandpowderymildewsusceptibility.Sharedtrichome-anddefense-relatedmutantphenotypessubcellularcolocalizationandisoform-preferentialprotein-proteininteractionindicateinterplayofEXO70andMLOproteinsinfocalsecretion.date2024sectionpartNumberpartTitleDOI10.1093plcellkoad319citationKeyurlPMIDPMCIDISSN1040-4651languagecollectionsQ65THHXGdateModified2025-03-19T115835Zkey4W3WF5NKlibraryid5891878metacreatorSummarySu00e1dlovu00e1etal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtSxE1dlovxE1J.YeoM.T.MateusD.S.PhelanJ.HaiL.A.BhattacharyyaT.KurtevS.SebestaO.MyskovaJ.SeblovaV.AnderssonB.SessionsP.F.deVolfP.MilesM.A.ltbgtComparativeGenomicsofltigtLeishmaniaDonovaniltigtProgenyfromGeneticCrossesinTwoSandFlySpeciesandImpactontheDiversityofDiagnosticandVaccineCandidatesltbgt.ltigtPLOSNEGLECTEDTROPICALDISEASESltigtltbgt2024ltbgtltigt18ltigt1.ltaclass039zp-DOIURL039href039httpsdoi.org10.1371journal.pntd.0011920039gthttpsdoi.org10.1371journal.pntd.0011920ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleComparativegenomicsofiLeishmaniadonovaniiprogenyfromgeneticcrossesintwosandflyspeciesandimpactonthediversityofdiagnosticandvaccinecandidatescreatorscreatorTypeauthorfirstNameJ.lastNameSu00e1dlovu00e1creatorTypeauthorfirstNameM.T.lastNameYeocreatorTypeauthorfirstNameD.S.lastNameMateuscreatorTypeauthorfirstNameJ.lastNamePhelancreatorTypeauthorfirstNameL.A.lastNameHaicreatorTypeauthorfirstNameT.lastNameBhattacharyyacreatorTypeauthorfirstNameS.lastNameKurtevcreatorTypeauthorfirstNameO.lastNameSebestacreatorTypeauthorfirstNameJ.lastNameMyskovacreatorTypeauthorfirstNameV.lastNameSeblovacreatorTypeauthorfirstNameB.lastNameAnderssoncreatorTypeauthorfirstNameP.F.delastNameSessionscreatorTypeauthorfirstNameP.lastNameVolfcreatorTypeauthorfirstNameM.A.lastNameMilesabstractNoteSandflytransmittedLeishmaniaspeciesareresponsibleforseverewiderangingvisceralandcutaneousleishmaniases.GeneticexchangecanoccuramongnaturalLeishmaniapopulationsandhybridscannowbeproducedexperimentallywithlimitations.FeedingPhlebotomusorientalisorPhlebotomusargentipesontwostrainsofLeishmaniadonovaniyieldedhybridprogenyselectedusingdoubledrugresistanceandfluorescencemarkers.Fluorescenceactivatedcellsortingofculturedclonesderivedfromthesehybridsindicateddiploidprogeny.Multilocussequencetypingoftheclonesshowedhybridisationandnuclearheterozygosityalthoughwithinheritanceofsinglehaplotypesinakinetoplastidtarget.Comparativegenomicsshoweddiversityofclonalprogenybetweensinglechromosomesandextraordinaryheterozygosityacrossall36chromosomes.DiversitybetweenprogenywasseenfortheHASPBantigenwhichhasbeennotedpreviouslyashavingimplicationsfordesignofatherapeuticvaccine.GenomicdiversityseenamongLeishmaniastrainsandhybridprogenyisofgreatimportanceinunderstandingtheepidemiologyandcontrolofleishmaniasis.AsanoutcomeofthisstudywestronglyrecommendthatwiderbiologicalarchivesofdifferentLeishmaniaspeciesfromendemicregionsshouldbeestablishedandmadeavailableforcomparativegenomics.Howeverinparallelperformanceofgeneticcrossesandgenomiccomparisonsshouldgivefundamentalinsightintothespecificitydiversityandlimitationsofcandidatediagnosticsvaccinesanddrugsfortargetedcontrolofleishmaniasis.date2024sectionpartNumberpartTitleDOI10.1371journal.pntd.0011920citationKeyurlPMIDPMCIDISSN1935-2735languagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeyHXAVKZGWlibraryid5891878metacreatorSummaryPanskaetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtPanskaL.NedvedovaS.VacekV.KrivskaD.KonecnyL.KnopF.KutilZ.SkultetyovaL.LeontovycA.UlrychovaL.SakanariJ.AsahinaM.BarinkaC.MacurkovaM.DvorakJ.ltbgtUncoveringtheEssentialRolesofGlutamateCarboxypeptidase2OrthologsinCaenorhabditisElegansltbgt.ltigtBIOSCIENCEREPORTSltigtltbgt2024ltbgtltigt44ltigt1.ltaclass039zp-DOIURL039href039httpsdoi.org10.1042BSR20230502039gthttpsdoi.org10.1042BSR20230502ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleUncoveringtheessentialrolesofglutamatecarboxypeptidase2orthologsinCaenorhabditiseleganscreatorscreatorTypeauthorfirstNameL.lastNamePanskacreatorTypeauthorfirstNameS.lastNameNedvedovacreatorTypeauthorfirstNameV.lastNameVacekcreatorTypeauthorfirstNameD.lastNameKrivskacreatorTypeauthorfirstNameL.lastNameKonecnycreatorTypeauthorfirstNameF.lastNameKnopcreatorTypeauthorfirstNameZ.lastNameKutilcreatorTypeauthorfirstNameL.lastNameSkultetyovacreatorTypeauthorfirstNameA.lastNameLeontovyccreatorTypeauthorfirstNameL.lastNameUlrychovacreatorTypeauthorfirstNameJ.lastNameSakanaricreatorTypeauthorfirstNameM.lastNameAsahinacreatorTypeauthorfirstNameC.lastNameBarinkacreatorTypeauthorfirstNameM.lastNameMacurkovacreatorTypeauthorfirstNameJ.lastNameDvorakabstractNoteHumanglutamatecarboxypeptidase2GCP2fromtheM28Bmetalloproteasegroupisanimportanttargetfortherapyinneurologicaldisordersandanestablishedtumormarker.Howeveritsphysiologicalfunctionsremainunclear.TobetterunderstandgeneralrolesweusedthemodelorganismCaenorhabditiseleganstogeneticallymanipulateitsthreeexistingorthologousgenesandevaluatetheimpactonwormphysiology.TheresultsofgeneknockoutstudiesshowedthatC.elegansGCP2orthologsaffectthepharyngealphysiologyreproductionandstructuralintegrityoftheorganism.Promoter-drivenGFPexpressionrevealeddistinctlocalizationforeachofthethreegeneparalogswithgcp-2.1beingmostabundantinmusclesintestineandpharyngealinterneuronsgcp-2.2restrictedtothephasmidneuronsandgcp-2.3locatedintheexcretorycell.ThepresentstudyprovidesnewinsightintotheuniquephenotypiceffectsofGCP2geneknockoutsinC.elegansandthespecifictissuelocalizations.Webelievethatelucidationofparticularrolesinanon-mammalianorganismcanhelptoexplainimportantquestionslinkedtophysiologyofthisproteasegroupandinextensiontohumanGCP2involvementinpathophysiologicalprocesses.date2024sectionpartNumberpartTitleDOI10.1042BSR20230502citationKeyurlPMIDPMCIDISSN0144-8463languagecollectionsQ65THHXGdateModified2025-03-19T115835ZkeyD4MQ5RFSlibraryid5891878metacreatorSummaryKubalovu00e1etal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKubalovxE1M.MxFCllerK.DobrevP.I.RizzaA.JonesA.M.FendrychM.ltbgtAuxinCo-ReceptorIAA17AXR3ControlsCellElongationinltigtArabidopsisThalianaltigtRootSolelybyModulationofNuclearAuxinPathwayltbgt.ltigtNEWPHYTOLOGISTltigtltbgt2024ltbgtltigt241ltigt62448x20132463.ltaclass039zp-DOIURL039href039httpsdoi.org10.1111nph.19557039gthttpsdoi.org10.1111nph.19557ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleAuxinco-receptorIAA17AXR3controlscellelongationiniArabidopsisthalianairootsolelybymodulationofnuclearauxinpathwaycreatorscreatorTypeauthorfirstNameM.lastNameKubalovu00e1creatorTypeauthorfirstNameK.lastNameMu00fcllercreatorTypeauthorfirstNameP.I.lastNameDobrevcreatorTypeauthorfirstNameA.lastNameRizzacreatorTypeauthorfirstNameA.M.lastNameJonescreatorTypeauthorfirstNameM.lastNameFendrychabstractNotecenterdotThenuclearTIR1AFB-AuxIAAauxinpathwayplaysacrucialroleinregulatingplantgrowthanddevelopment.SpecificallytheIAA17AXR3proteinparticipatesinArabidopsisthalianarootdevelopmentresponsetoauxinandgravitropism.HoweverthemechanismbywhichAXR3regulatescellelongationisnotfullyunderstood.centerdotWecombinedgeneticalandcellbiologicaltoolswithtranscriptomicsanddeterminationofauxinlevelsandemployedlivecellimagingandimageanalysistoaddresshowtheauxinresponsepathwaysinfluencethedynamicsofrootgrowth.centerdotWerevealedthatmanipulationsoftheTIR1AFB-AuxIAApathwayrapidlymodulaterootcellelongation.WhileinducibleoverexpressionoftheAXR3-1transcriptionalinhibitoracceleratedgrowthoverexpressionofthedominantactivatorformofARF5MONOPTEROSinhibitedgrowth.InparallelAXR3-1expressioncausedlossofauxinsensitivityleadingtotranscriptionalreprogrammingphytohormonesignalingimbalanceandincreasedlevelsofauxin.FurthermorewedemonstratedthatAXR3-1specificallyperturbsnuclearauxinsignalingwhiletherapidauxinresponseremainsfunctional.centerdotOurresultsshedlightontheinterplaybetweenthenuclearandcytoplasmicauxinpathwaysinrootsrevealingtheirpartialindependencebutalsothedominantroleofthenuclearauxinpathwayduringthegravitropicresponseofArabidopsisthalianaroots.date2024sectionpartNumberpartTitleDOI10.1111nph.19557citationKeyurlPMIDPMCIDISSN0028-646XlanguagecollectionsQ65THHXGdateModified2025-03-19T115834ZkeyVPBBNWGJlibraryid5891878metacreatorSummaryFrontinoetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtFrontinoA.M.OliveiraD.V.TrampotovxE1E.TavakoliM.HulN.K.M.V.GozlanO.AnderssonE.PavlinkovxE1G.SprinzakD.MasekJ.ltbgtPronouncedVasculaturePhenotypesinNewMouseModelswithALGSandBA-AssociatedMutationsinJag1ltbgt.ltigtJOURNALOFHEPATOLOGYltigtltbgt2024ltbgtltigt80ltigtEuropean-Association-for-the-Study-of-the-LiverCongressEASLS697x2013S698.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitlePronouncedvasculaturephenotypesinnewmousemodelswithALGSandBA-associatedmutationsinJag1creatorscreatorTypeauthorfirstNameA.M.lastNameFrontinocreatorTypeauthorfirstNameD.V.lastNameOliveiracreatorTypeauthorfirstNameE.lastNameTrampotovu00e1creatorTypeauthorfirstNameM.lastNameTavakolicreatorTypeauthorfirstNameN.K.M.VanlastNameHulcreatorTypeauthorfirstNameO.lastNameGozlancreatorTypeauthorfirstNameE.lastNameAnderssoncreatorTypeauthorfirstNameG.lastNamePavlinkovu00e1creatorTypeauthorfirstNameD.lastNameSprinzakcreatorTypeauthorfirstNameJ.lastNameMasekabstractNotedate2024sectionpartNumberpartTitleDOIcitationKeyurlPMIDPMCIDISSN0168-8278languagecollectionsQ65THHXGdateModified2025-03-19T115834ZkeyE8IFRCYBlibraryid5891878metacreatorSummaryPeterkovu00e1etal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtPeterkovxE1K.KonecnyL.MachxE1cekT.JedlickovxE1L.WinkelmannF.SombetzkiM.DvorxE1kJ.ltbgtWinnersvs.LosersltigtSchistosomaMansoniltigtIntestinalandLiverEggsExhibitStrikingDifferencesinGeneExpressionandImmunogenicityltbgt.ltigtPLOSPATHOGENSltigtltbgt2024ltbgtltigt20ltigt5.ltaclass039zp-DOIURL039href039httpsdoi.org10.1371journal.ppat.1012268039gthttpsdoi.org10.1371journal.ppat.1012268ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleWinnersvs.losersiSchistosomamansoniiintestinalandlivereggsexhibitstrikingdifferencesingeneexpressionandimmunogenicitycreatorscreatorTypeauthorfirstNameK.lastNamePeterkovu00e1creatorTypeauthorfirstNameL.lastNameKonecnycreatorTypeauthorfirstNameT.lastNameMachu00e1cekcreatorTypeauthorfirstNameL.lastNameJedlickovu00e1creatorTypeauthorfirstNameF.lastNameWinkelmanncreatorTypeauthorfirstNameM.lastNameSombetzkicreatorTypeauthorfirstNameJ.lastNameDvoru00e1kabstractNoteTheeggsofthebloodflukeSchistosomamansoniarethemaincauseoftheclinicalmanifestationsofchronicschistosomiasis.Afterlayingtheeggquotwinnersquotattachtotheendotheliumofthemesentericveinandafteraperiodofdevelopmentinducethegrowthofasmallgranulomawhichfacilitatestheirpassagetotheintestinallumen.Eggquotlosersquotcarriedbythebloodstreamtonon-specifictissuesalsoundergofulldevelopmentandinducelargegranulomaformationbuttheirlifeendsthere.AlthoughthesetrappedeggsrepresentadeadendintheparasitelifecyclethevastmajorityofstudiesattemptingtodescribethebiologyoftheS.mansonieggshavestudiedtheseliver-trappedquotlosersquotinsteadofmigratingintestinalquotwinnersquot.Thisraisesthefundamentalquestionofhowtheseeggsdiffer.WithrobustcomparativetranscriptomicanalysisperformedonS.mansonieggsisolated7weekspostinfectionweshowthatgeneexpressioniscriticallydependentontissuelocalizationbothintheearlyandlatestagesofdevelopment.Whilemitochondrialgenesandvenomallergen-likeproteinsaresignificantlyupregulatedinmatureintestinaleggswell-describedeggimmunomodulatorsIPSEalpha-1andomega-1togetherwithmicro-exongenesarepredominantlyexpressedinlivereggs.Inadditionseveralproteasesandproteaseinhibitorspreviouslyimplicatedinegg-hostinteractionsdisplaycleartissue-specificgeneexpressionpatterns.Thesemajordifferencesingeneexpressioncouldbethenreflectedintheobserveddifferentabilityofliverandintestinalsolubleeggantigenstoelicithostimmuneresponsesandintheshorterviabilityofmiracidiahatchedfromlivereggs.Ourcomparativeanalysisprovidesanewperspectiveonthebiologyofparasite039seggsinthecontextoftheirdevelopmentandtissuelocalization.Thesefindingscouldcontributetoabroaderandmoreaccurateunderstandingofparasiteeggsinteractionswiththehostwhichhavehistoricallybeenoftenrestrictedtolivereggsandsometimesinaccuratelygeneralized.date2024sectionpartNumberpartTitleDOI10.1371journal.ppat.1012268citationKeyurlPMIDPMCIDISSN1553-7366languagecollectionsQ65THHXGdateModified2025-03-19T115834ZkeyRWUUL5FTlibraryid5891878metacreatorSummaryGhavietal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtGhaviF.P.GolisP.KubuM.PrechJ.OpanasenkoM.ltbgtAcidityandPorosityPropertiesofZeolitesAffectTheirCatalyticPerformanceinThymolSynthesisltbgt.ltigtMICROPOROUSANDMESOPOROUSMATERIALSltigtltbgt2024ltbgtltigt376ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.micromeso.2024.113198039gthttpsdoi.org10.1016j.micromeso.2024.113198ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleAcidityandporositypropertiesofzeolitesaffecttheircatalyticperformanceinthymolsynthesiscreatorscreatorTypeauthorfirstNameF.P.lastNameGhavicreatorTypeauthorfirstNameP.lastNameGoliscreatorTypeauthorfirstNameM.lastNameKubucreatorTypeauthorfirstNameJ.lastNamePrechcreatorTypeauthorfirstNameM.lastNameOpanasenkoabstractNoteThymolissynthesizedfromm-cresolalkylationreactionwith2-propanol.Afewcommercialzeoliteshavebeentestedinthisreactionbuttheinfluenceofacidityandporosityofazeoliteonthem-cresolconversionandthymolyieldisscarcelystudied.Isomorphously-substitutedAl-Ga-Fe-B-andIn-UTLwithdifferentheteroelementsandthusdifferentratiobetweenstrongandweaksitesgiveinsightintotheeffectofacidity.IsoreticularzeolitesAl-UTLAl-IPC-7andAl-IPC-2withdifferentporesizesrevealtheeffectofporosity.InthisstudymcresolconversionwasfarfromcomparableoverAl-UTLorGa-UTLX18-19andFe-UTLB-UTLorIn-UTLX2-3havingdifferentconcentrationofstrongBrphinstedacidsites.ThymolyieldwasalsohigheroverAlandGa-UTLY11-13thanoverFe-B-andIn-UTLY1-2.TheeasieraccessibilitytothestrongBrphinstedacidsitesinAl-UTLprovidedbybiggerporesizesresultedinhigherm-cresolconversionandthymolyieldcomparedtoAl-IPC-7X15andY8andAl-IPC-2X6andY2.InadditionthestrongerBrphinstedacidityandlargeandextra-largeporosityinAl-UTLandGa-UTLfacilitatedtherearrangementofisopropyl-3-methylphenylether-theproductoftheO-alkylationpathway-tothymol.ThusAl-UTLandGaUTLalongwithAl-FAUreferencematerialconfirmedtheoptimumstructuralpropertiesforahighselectivitytothymol.date2024sectionpartNumberpartTitleDOI10.1016j.micromeso.2024.113198citationKeyurlPMIDPMCIDISSN1387-1811languagecollectionsQ65THHXGdateModified2025-03-19T115834ZkeyJJH2ZFVLlibraryid5891878metacreatorSummaryKurbanovaetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKurbanovaA.ZxE1kutnxE1D.GolabekK.HranxEDcekJ.DugulanA.I.DiddamsP.HsiehM.F.BatsN.PrechJ.ltbgtFe-ZSM-5OutperformsAl-ZSM-5inParaffinCrackingbyIncreasingtheOlefinicityofCltsubgt3ltsubgt-Cltsubgt4ltsubgtProductsltbgt.ltigtCHEMICALENGINEERINGJOURNALltigtltbgt2024ltbgtltigt499ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.cej.2024.156032039gthttpsdoi.org10.1016j.cej.2024.156032ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleFe-ZSM-5outperformsAl-ZSM-5inparaffincrackingbyincreasingtheolefinicityofCsub3sub-Csub4subproductscreatorscreatorTypeauthorfirstNameA.lastNameKurbanovacreatorTypeauthorfirstNameD.lastNameZu00e1kutnu00e1creatorTypeauthorfirstNameK.lastNameGolabekcreatorTypeauthorfirstNameJ.lastNameHranu00edcekcreatorTypeauthorfirstNameA.I.lastNameDugulancreatorTypeauthorfirstNameP.lastNameDiddamscreatorTypeauthorfirstNameM.F.lastNameHsiehcreatorTypeauthorfirstNameN.lastNameBatscreatorTypeauthorfirstNameJ.lastNamePrechabstractNoteIron-modifiedAl-ZSM-5increasesselectivitytopropeneakeypetrochemicalresultingfromfluidcatalyticcrackingFCC.HoweverthetypeandroleofactiveironspeciesremainunclearhinderingeffortstostreamlinethedesignofselectiveFCCadditives.HereweinvestigatedAl-freeFe-ZSM-5catalystscontainingironspeciesintheformofframeworkFe3extra-frameworkFe3oxidicclustersandoxidemicroaggregatesinn-octanecrackingFCCmodeltoassesstheireffectoncatalyticcracking.DR-UV-VisspectroscopyFe-57MampoumlssbauerSpectroscopyFTIRstudiesofpyridineadsorptionandn-octanecrackingtestsat500degreesCrevealedthatframework-associatedcoordinativelyunsaturatedFe3specieswhichinducestrongLewisacidityareresponsibleforparaffincrackinginitiationwhereasbulkironoxidesonthezeolitesurfaceareinactive.IncomparisonwithAl-ZSM-5Fe-ZSM-5increasestheolefinicityofthevaluableC-3-C-4fractionsselectivitytopropeneandbutenesandpromotesaromatizationreactionsduetothelowerrelativestrengthofFe-inducedBramposlashnstedacidsitesanddehydrogenationproperties.AsshownbyourFe-57Mampoumlssbauerstudyperformedat-269degreesCofthecatalystincalcinedspentandregeneratedstatesFe-ZSM-5deactivationisassociatedwiththelossoftetrahedrallycoordinatedFe3species.ThereforetuningFe-ZSM-5C-3-C-4selectiveFCCadditivesrequiresstabilizingframeworkBramposlashnstedandframework-associatedLewisacidsiteswhiledecreasingtheconcentrationofironoxidespecies.UltimatelythesefindingsmayenableustomeetthedemandforpropenederivedfromFCCcrackingwhichisexpectedtogrowintheforeseeablefuture.date2024sectionpartNumberpartTitleDOI10.1016j.cej.2024.156032citationKeyurlPMIDPMCIDISSN1385-8947languagecollectionsQ65THHXGdateModified2025-03-19T115834Zkey3I4SMCCHlibraryid5891878metacreatorSummaryKnopetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKnopF.ZounarovxE1A.SabataV.MiddelkoopT.C.MacurkovxE1M.ltbgtCaenorhabditisElegansSEL-5AAK1RegulatesCellMigrationandCellOutgrowthIndependentlyofItsKinaseActivityltbgt.ltigtELIFEltigtltbgt2024ltbgtltigt13ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.7554eLife.91054039gthttpsdoi.org10.7554eLife.91054ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleCaenorhabditiselegansSEL-5AAK1regulatescellmigrationandcelloutgrowthindependentlyofitskinaseactivitycreatorscreatorTypeauthorfirstNameF.lastNameKnopcreatorTypeauthorfirstNameA.lastNameZounarovu00e1creatorTypeauthorfirstNameV.lastNameSabatacreatorTypeauthorfirstNameT.C.lastNameMiddelkoopcreatorTypeauthorfirstNameM.lastNameMacurkovu00e1abstractNoteDuringCaenorhabditiselegansdevelopmentmultiplecellsmigratelongdistancesorextendprocessestoreachtheirfinalpositionandorattainpropershape.TheWntsignallingpathwaystandsoutasoneofthemajorcoordinatorsofcellmigrationorcelloutgrowthalongtheanterior-posteriorbodyaxis.TheoutcomeofWntsignallingisfine-tunedbyvariousmechanismsincludingendocytosis.InthisstudyweshowthatSEL-5theC.elegansorthologueofmammalianAP2-associatedkinaseAAK1actstogetherwiththeretromercomplexasapositiveregulatorofEGL-20WntsignallingduringthemigrationofQLneuroblastdaughtercells.AtthesametimeSEL-5incooperationwiththeretromercomplexisalsorequiredduringexcretorycanalcelloutgrowth.ImportantlySEL-5kinaseactivityisnotrequiredforitsroleinneuronalmigrationorexcretorycelloutgrowthandneitheroftheseprocessesisdependentonDPY-23AP2M1phosphorylation.WefurtherestablishthattheWntproteinsCWN-1andCWN-2togetherwiththeFrizzledreceptorCFZ-2positivelyregulateexcretorycelloutgrowthwhileLIN-44WntandLIN-17Frizzledtogethergenerateastopsignalinhibitingitsextension.date2024sectionpartNumberpartTitleDOI10.7554eLife.91054citationKeyurlPMIDPMCIDISSN2050-084XlanguagecollectionsQ65THHXGdateModified2025-03-19T115834ZkeyWY4VZ63Wlibraryid5891878metacreatorSummaryLiskaetal.parsedDate2024numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtLiskaV.WillimetzR.KubxE1tP.KrtenovxE1P.GyepesR.MosingerJ.ltbgtSynergisticPhotogenerationofNitricOxideandSingletOxygenbyNanofiberMembranesviaBlueandorRed-LightIrradiationStrongAntibacterialActionltbgt.ltigtJOURNALOFPHOTOCHEMISTRYANDPHOTOBIOLOGYB-BIOLOGYltigtltbgt2024ltbgtltigt255ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.jphotobiol.2024.112906039gthttpsdoi.org10.1016j.jphotobiol.2024.112906ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleSynergisticphotogenerationofnitricoxideandsingletoxygenbynanofibermembranesviablueandorred-lightirradiationStrongantibacterialactioncreatorscreatorTypeauthorfirstNameV.lastNameLiskacreatorTypeauthorfirstNameR.lastNameWillimetzcreatorTypeauthorfirstNameP.lastNameKubu00e1tcreatorTypeauthorfirstNameP.lastNameKrtenovu00e1creatorTypeauthorfirstNameR.lastNameGyepescreatorTypeauthorfirstNameJ.lastNameMosingerabstractNoteNewfunctionalitieswereaddedtobiocompatiblepolycaprolactonenanofibermaterialsthroughthecoencapsulationofchlorine6trimethylesterCe6photogeneratingsingletoxygenandabsorbinglightbothintheblueandredregionsandusing4-N-aminopropyl-3-trifluoromethyl-4-nitrobenzenamine-7-nitrobenzofurazanNO-photodonorNOPabsorbinglightintheblueregionofvisiblelight.Time-resolvedandsteady-stateluminescenceaswellasabsorptionspectroscopywereusedtomonitorbothphotoactivecompounds.Thenanofibermaterialexhibitedphotogenerationofantibacterialspeciesspecificallynitricoxideandsingletoxygenuponvisiblelightexcitation.ThisprocessresultedintheefficientphotodynamicinactivationofE.colinotonlyclosetonanofibermaterialsurfacesduetoshort-livedsingletoxygenbutevenatlongerdistancesduetodiffusionoflonger-livednitricoxide.InterestinglynitricoxidewasalsoformedbyprocessesinvolvingphotosensitizationofCe6duringirradiationbyredlight.ThisispromisingfornumerousapplicationsespeciallyinthebiomedicalfieldwherestrictlylocalphotogenerationofNOanditstherapeuticbenefitscanbeappliedusingexcitationinthequothumanbodyphototherapeuticwindowquot600-850nm.GenerallyduetothehighpermeabilityofredlightthephotogenerationofNOcanbeachievedinanyaqueousenvironmentwheredirectexcitationofNOPtoitsabsorbanceintheblueregionislimited.date2024sectionpartNumberpartTitleDOI10.1016j.jphotobiol.2024.112906citationKeyurlPMIDPMCIDISSN1011-1344languagecollectionsQ65THHXGdateModified2025-03-19T115834Z
1.
Zahradníčková, V.; Hovořáková, M.; Tucker, A. S.; Bartoš, M.; Rehák, I.; Zahradníček, O. Postnatal Dentition Changes in the Cuban False Chameleons: Adaptation to a Dietary Shift. fozo.1 2024, 73 (24063), 24063.1-20. https://doi.org/10.25225/jvb.24063.
1.
Vašek, D.; Fikarová, N.; Marková, V. N.; Honc, O.; Pacáková, L.; Porubská, B.; Somova, V.; Novotný, J.; Melkes, B.; Krulová, M. Lipopolysaccharide Pretreatment Increases the Sensitivity of the TRPV1 Channel and Promotes an Anti-Inflammatory Phenotype of Capsaicin-Activated Macrophages. Journal of Inflammation 2024, 21 (1), 17. https://doi.org/10.1186/s12950-024-00391-0.
1.
Eliášová, P.; Šmíd, B.; Vejpravová, J.; Li, S.; Brivio, F.; Mazur, M.; Rainer, D. N.; Mohideen, M. I. H.; Morris, R. E.; Nachtigall, P. The Preparation Route and Final Form of V-MXenes Override the Effect of the O/F Ratio on Their Magnetic Properties. J. Mater. Chem. C 2024, 12 (15), 5431–5441. https://doi.org/10.1039/D4TC00132J.
1.
Zumr, V.; Nakladal, O.; Remes, J. Deadwood-Dwelling Beetles (Coleoptera: Eucnemidae) in a Beech Reserve: A Case Study from the Czech Republic. Forests 2024, 15 (3), 469. https://doi.org/10.3390/f15030469.
1.
Thakur, H.; Agarwal, S.; Bucek, A.; Hradecky, J.; Sehadova, H.; Mathur, V.; Togaev, U.; van de Kamp, T.; Hamann, E.; Liu, R.-H.; Verma, K. S.; Li, H.-F.; Sillam-Duss, D.; Engel, M. S.; Sobotnik, J. Defensive Glands in Stylotermitidae (Blattodea, Isoptera). Arthropod Struct. Dev. 2024, 79, 101346. https://doi.org/10.1016/j.asd.2024.101346.
1.
Muster, C.; Korba, J.; Bogusch, P.; Heneberg, P.; Šťáhlavský, F. And Yet They Differ: Reconsiderations of Diversity within Dactylochelifer Latreillii (Arachnida: Pseudoscorpiones). Diversity 2024, 16 (3), 137. https://doi.org/10.3390/d16030137.
1.
Foucková, M.; Uhrová, K.; Kubánková, A.; Pánek, T.; Cepicka, I. Lighting Lantern above Psalteriomonadidae: Unveiling Novel Diversity within the Genus Psalteriomonas (Discoba: Heterolobosea). EUROPEAN JOURNAL OF PROTISTOLOGY 2024, 93. https://doi.org/10.1016/j.ejop.2024.126052.
1.
Hoboth, P.; Sztacho, M.; Hozák, P. Nuclear Patterns of Phosphatidylinositol 4,5-and 3,4-Bisphosphate Revealed by Super-Resolution Microscopy Differ between the Consecutive Stages of RNA Polymerase II Transcription. FEBS JOURNAL 2024, 291 (19), 4240–4264. https://doi.org/10.1111/febs.17136.
1.
Soukup, J.; Zelená, M.; Weisz, F.; Kostelanská, M.; Nohynková, E.; Tumová, P. Imaging Giardia Intestinalis Cellular Organisation Using Expansion Microscopy Reveals Atypical Centrin Localisation. EXPERIMENTAL PARASITOLOGY 2024, 266. https://doi.org/10.1016/j.exppara.2024.108831.
1.
Ali, E.; Červenková, L.; Pálek, R.; Ambrozkiewicz, F.; Hošek, P.; Daum, O.; Liška, V.; Hemminki, K.; Trailin, A. Prognostic Role of Macrophages and Mast Cells in the Microenvironment of Hepatocellular Carcinoma after Resection. BMC Cancer 2024, 24 (1), 142. https://doi.org/10.1186/s12885-024-11904-8.
1.
Bourland, W.; Pomahac, O.; Cepicka, I. Redescription and Molecular Phylogeny of the Freshwater Metopid, Castula Strelkowi (Jankowski, 1964) from the Czech Republic and Synonymization of Pileometopus with Castula. PROTIST 2024, 175 (3). https://doi.org/10.1016/j.protis.2024.126034.
1.
Sabo, J.; Zdimalova, M. D.; Slater, P. G.; Dostal, V.; Herynek, S.; Libusova, L.; Lowery, L. A.; Braun, M.; Lansky, Z. CKAP5 Enables Formation of Persistent Actin Bundles Templated by Dynamically Instable Microtubules. CURRENT BIOLOGY 2024, 34 (2). https://doi.org/10.1016/j.cub.2023.11.031.
1.
Fleischhacker-Daffert, C.; Zerobin, A.; Hummel, F.; Slaninova, E.; Kroupova, Z.; Obruca, S.; Mrazova, K.; Hrubanova, K.; Krzyzanek, V.; Nebesarova, J.; Ludwig, K.; Fritz, I. A Comparison of the Effects of Continuous Illumination and Day/Night Regimes on PHB Accumulation in Synechocystis Cells. LIFE-BASEL 2024, 14 (7). https://doi.org/10.3390/life14070907.
1.
Ali, E.; Cervenková, L.; Pálek, R.; Ambrozkiewicz, F.; Pavlov, S.; Ye, W. J.; Hosek, P.; Daum, O.; Liska, V.; Hemminki, K.; Trailin, A. Mast Cells in the Microenvironment of Hepatocellular Carcinoma Confer Favorable Prognosis: A Retrospective Study Using QuPath Image Analysis Software. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS 2024, No. 206. https://doi.org/10.3791/66743.
1.
Zhang, J.; Yue, Q. D.; Shamma, E.; Abdi, S.; Petrov, O.; Cejka, J.; Mintova, S.; Opanasenko, M.; Shamzhy, M. Balancing Ge De-Intercalation and Si Re-Insertion Rates Stabilizes Hydrolytically Labile Germanosilicate Zeolites. JOURNAL OF MATERIALS CHEMISTRY A 2024, 12 (45), 31195–31203. https://doi.org/10.1039/d4ta05539j.
1.
Schrecengost, A.; Rotterová, J.; Poláková, K.; Cepicka, I.; Beinart, R. A. Divergent Marine Anaerobic Ciliates Harbor Closely Related Methanocorpusculum Endosymbionts. ISME JOURNAL 2024, 18 (1). https://doi.org/10.1093/ismejo/wrae125.
1.
Roucová, K.; Vopálensky, V.; Masek, T.; Llano, E. D.; Provazník, J.; Landry, J. J. M.; Azevedo, N.; Ehler, E.; Benes, V.; Pospísek, M. Loss of ADAR1 Protein Induces Changes in Small RNA Landscape in Hepatocytes. RNA 2024, 30 (9), 1164–1183. https://doi.org/10.1261/rna.080097.124.
1.
Masek, J.; Filipovic, I.; Hul, N. V.; Belicova, L.; Jirousková, M.; Oliveira, D.; Frontino, A. M.; Hankeova, S.; He, J. Y.; Turetti, F.; Iqbal, A.; Cervenka, I.; Sarnova, L.; Verboven, E.; Brabec, T.; Bjoerkström, N. K.; Gregor, M.; Dobes, J.; Andersson, E. R. Jag1 Insufficiency Alters Liver Fibrosis via T Cell and Hepatocyte Differentiation Defects. EMBO MOLECULAR MEDICINE 2024, 16 (11), 2946–2975. https://doi.org/10.1038/s44321-024-00145-8.
1.
Huber, B. A.; Meng, G. L.; Dederichs, T. M.; Michalik, P.; Forman, M.; Král, J. Castaways: The Leeward Antilles Endemic Spider Genus Papiamenta (Araneae: Pholcidae). INVERTEBRATE SYSTEMATICS 2024, 38 (2). https://doi.org/10.1071/IS23052.
1.
Huebbers, J. W.; Caldarescu, G. A.; Kubátová, Z.; Sabol, P.; Levecque, S. C. J.; Kuhn, H.; Kulich, I.; Reinstädler, A.; Büttgen, K.; Manga-Robles, A.; Mélida, H.; Pauly, M.; Panstruga, R.; Zársky, V. Interplay of EXO70 and MLO Proteins Modulates Trichome Cell Wall Composition and Susceptibility to Powdery Mildew. PLANT CELL 2024. https://doi.org/10.1093/plcell/koad319.
1.
Sádlová, J.; Yeo, M. T.; Mateus, D. S.; Phelan, J.; Hai, L. A.; Bhattacharyya, T.; Kurtev, S.; Sebesta, O.; Myskova, J.; Seblova, V.; Andersson, B.; Sessions, P. F. de; Volf, P.; Miles, M. A. Comparative Genomics of Leishmania Donovani Progeny from Genetic Crosses in Two Sand Fly Species and Impact on the Diversity of Diagnostic and Vaccine Candidates. PLOS NEGLECTED TROPICAL DISEASES 2024, 18 (1). https://doi.org/10.1371/journal.pntd.0011920.
1.
Panska, L.; Nedvedova, S.; Vacek, V.; Krivska, D.; Konecny, L.; Knop, F.; Kutil, Z.; Skultetyova, L.; Leontovyc, A.; Ulrychova, L.; Sakanari, J.; Asahina, M.; Barinka, C.; Macurkova, M.; Dvorak, J. Uncovering the Essential Roles of Glutamate Carboxypeptidase 2 Orthologs in Caenorhabditis Elegans. BIOSCIENCE REPORTS 2024, 44 (1). https://doi.org/10.1042/BSR20230502.
1.
Kubalová, M.; Müller, K.; Dobrev, P. I.; Rizza, A.; Jones, A. M.; Fendrych, M. Auxin Co-Receptor IAA17/AXR3 Controls Cell Elongation in Arabidopsis Thaliana Root Solely by Modulation of Nuclear Auxin Pathway. NEW PHYTOLOGIST 2024, 241 (6), 2448–2463. https://doi.org/10.1111/nph.19557.
1.
Frontino, A. M.; Oliveira, D. V.; Trampotová, E.; Tavakoli, M.; Hul, N. K. M. V.; Gozlan, O.; Andersson, E.; Pavlinková, G.; Sprinzak, D.; Masek, J. Pronounced Vasculature Phenotypes in New Mouse Models with ALGS and BA-Associated Mutations in Jag1. JOURNAL OF HEPATOLOGY 2024, 80 (European-Association-for-the-Study-of-the-Liver Congress (EASL)), S697–S698.
1.
Peterková, K.; Konecny, L.; Machácek, T.; Jedlicková, L.; Winkelmann, F.; Sombetzki, M.; Dvorák, J. Winners vs. Losers: Schistosoma Mansoni Intestinal and Liver Eggs Exhibit Striking Differences in Gene Expression and Immunogenicity. PLOS PATHOGENS 2024, 20 (5). https://doi.org/10.1371/journal.ppat.1012268.
1.
Ghavi, F. P.; Golis, P.; Kubu, M.; Prech, J.; Opanasenko, M. Acidity and Porosity Properties of Zeolites Affect Their Catalytic Performance in Thymol Synthesis. MICROPOROUS AND MESOPOROUS MATERIALS 2024, 376. https://doi.org/10.1016/j.micromeso.2024.113198.
1.
Kurbanova, A.; Zákutná, D.; Golabek, K.; Hranícek, J.; Dugulan, A. I.; Diddams, P.; Hsieh, M. F.; Bats, N.; Prech, J. Fe-ZSM-5 Outperforms Al-ZSM-5 in Paraffin Cracking by Increasing the Olefinicity of C3-C4 Products. CHEMICAL ENGINEERING JOURNAL 2024, 499. https://doi.org/10.1016/j.cej.2024.156032.
1.
Knop, F.; Zounarová, A.; Sabata, V.; Middelkoop, T. C.; Macurková, M. Caenorhabditis Elegans SEL-5/AAK1 Regulates Cell Migration and Cell Outgrowth Independently of Its Kinase Activity. ELIFE 2024, 13. https://doi.org/10.7554/eLife.91054.
1.
Liska, V.; Willimetz, R.; Kubát, P.; Krtenová, P.; Gyepes, R.; Mosinger, J. Synergistic Photogeneration of Nitric Oxide and Singlet Oxygen by Nanofiber Membranes via Blue and/or Red-Light Irradiation: Strong Antibacterial Action. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2024, 255. https://doi.org/10.1016/j.jphotobiol.2024.112906.
2023
5891878
MPJ5STUG
1
https://raw.githubusercontent.com/Schebique/vmcf-konfmi/refs/heads/main/vmcf-web-style.csl
50
date
desc
4983
https://web.natur.cuni.cz/sekce-bi/VMCF/wp-content/plugins/zotpress/
statussuccessupdateneededfalseinstancefalsemetarequest_last0request_next0used_cachetruedatakeyWHNL6WFZlibraryid5891878metacreatorSummaryThakuretal.parsedDate2023-12-01numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtThakurH.AgarwalS.HradeckxFDJ.SharmaG.LiH.-F.YangS.-E.SehadovxE1H.ChandelR.S.Hylix161M.MathurV.x160obotnxEDkJ.Sillam-DussxE8sD.ltbgtTheTrail-FollowingCommunicationinStylotermesFaveolusandS.HalumicusBlattodeaIsopteraStylotermitidaeltbgt.ltigtJChemEcolltigtltbgt2023ltbgtltigt49ltigt11642x2013651.ltaclass039zp-ItemURL039href039httpsdoi.org10.1007s10886-023-01447-w039gthttpsdoi.org10.1007s10886-023-01447-wltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTheTrail-FollowingCommunicationinStylotermesfaveolusandS.halumicusBlattodeaIsopteraStylotermitidaecreatorscreatorTypeauthorfirstNameHimanshulastNameThakurcreatorTypeauthorfirstNameSurbhilastNameAgarwalcreatorTypeauthorfirstNameJaromu00edrlastNameHradecku00fdcreatorTypeauthorfirstNameGarimalastNameSharmacreatorTypeauthorfirstNameHou-FenglastNameLicreatorTypeauthorfirstNameShang-EnlastNameYangcreatorTypeauthorfirstNameHanalastNameSehadovu00e1creatorTypeauthorfirstNameRavinderS.lastNameChandelcreatorTypeauthorfirstNameMireklastNameHyliu0161creatorTypeauthorfirstNameVartikalastNameMathurcreatorTypeauthorfirstNameJanlastNameu0160obotnu00edkcreatorTypeauthorfirstNameDavidlastNameSillam-Dussu00e8sabstractNoteStylotermitidaeappearpeculiaramongalltermitesfeedingintrunksoflivingtreesinSouthAsiaonly.Thedifficultytocollectthemlimitstheabilitytostudythemandtheythusstillbelongtocriticallyunknowngroupsinrespecttotheirbiology.Weusedacombinationofmicroscopicobservationschemicalanalysisandbehaviouralteststodeterminethesourceandchemicalnatureofthetrail-followingpheromoneofStylotermesfaveolusfromIndiaandS.halumicusfromTaiwan.Thesternalglandlocatedatthe5thabdominalsegmentwastheexclusivesourceofthetrail-followingpheromoneinbothS.faveolusandS.halumicusanditismadeupofclassIIIandIIIsecretorycells.Usinggaschromatographycoupledmassspectrometry3Z-dodec-3-en-1-olDOEwasidentifiedasthetrail-followingpheromonewhichelicitsstrongbehaviouralresponsesinworkersatathresholdaround10u2212u20094ngcmand0.1nggland.Ourresultsconfirmtheswitchfromcomplexaldehydetrail-followingpheromonesoccurringinthebasalgroupstosimplerlinearalcoholsintheancestorofKalotermitidaeandNeoisoptera.date2023-12-01sectionpartNumberpartTitleDOI10.1007s10886-023-01447-wcitationKeyurlhttpsdoi.org10.1007s10886-023-01447-wPMIDPMCIDISSN1573-1561languageencollectionsMPJ5STUGdateModified2025-11-07T090024Zkey5SQJIFU8libraryid5891878metacreatorSummaryNemcovaandDiaz-PulidoparsedDate2023-11-19numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtNemcovaY.Diaz-PulidoG.ltbgtFloristicandEcologicalInsightsintoSilica-ScaledChrysophytesinSoutheasternQueenslandAustralialtbgt.ltigtPlantSystEvolltigtltbgt2023ltbgtltigt309ltigt643.ltaclass039zp-ItemURL039href039httpsdoi.org10.1007s00606-023-01881-z039gthttpsdoi.org10.1007s00606-023-01881-zltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleFloristicandecologicalinsightsintosilica-scaledchrysophytesinsoutheasternQueenslandAustraliacreatorscreatorTypeauthorfirstNameYvonnelastNameNemcovacreatorTypeauthorfirstNameGuillermolastNameDiaz-PulidoabstractNoteSilica-scaledchrysophytesincludingseverallineagesoftheclassChrysophyceaepossessmorphologicallydiversesilicascales.Despitetheirecologicalsignificancefewdataontheirdistributionareavailablefromthisregion.Inthisstudyweaimedtoexplorethediversityanddistributionofsilica-scaledchrysophytesinsoutheasternQueensland.Sampleswerecollectedfromdifferentwaterbodiesandscalemorphologywasexaminedusingtransmissionandscanningelectronmicroscopy.Waterchemicalanalyseswereconductedtorevealtheecologicalrequirementsofthespecies.Atotalof35taxawereidentifiedincludingspeciesfromtheordersSynuralesChromulinalesandParaphysomonadales.MallomonaspseudocoronataoriginallythoughttobeanendemicofNorthAmericaandlaterrecordedfromEuropewasfoundinQueensland.Ourfindingsprovidevaluablefloristicdataandcontributetotheunderstandingofsilica-scaledchrysophytesdistributioninAustralia.Molecularcharacterizationofthesespeciesiscrucialtoassessendemismandfurtheradvancechrysophyteresearchintheregion.date2023-11-19sectionpartNumberpartTitleDOI10.1007s00606-023-01881-zcitationKeyurlhttpsdoi.org10.1007s00606-023-01881-zPMIDPMCIDISSN1615-6110languageencollectionsMPJ5STUGdateModified2025-11-07T085202ZkeyS2Q645TGlibraryid5891878metacreatorSummaryPau010desetal.parsedDate2023-11numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtPax10DesJ.GrobxE1rovxE1V.Zadrax17EilZ.KnxEDx17EkovxE1K.MalinskxE1N.Tux161kovxE1L.BoesM.x10CernxFDJ.ltbgtMHCIIx2013EGFPKnock-inMouseModelltbgt.ltigtCurrentProtocolsltigtltbgt2023ltbgtltigt3ltigt11e925.httpsdoi.orgltaclass039zp-ItemURL039href039httpsdoi.org10.1002cpz1.925039gthttpsdoi.org10.1002cpz1.925ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMHCIIu2013EGFPKnock-inMouseModelcreatorscreatorTypeauthorfirstNameJanlastNamePau010descreatorTypeauthorfirstNameValu00e9rialastNameGrobu00e1rovu00e1creatorTypeauthorfirstNameZdenu011bklastNameZadrau017eilcreatorTypeauthorfirstNameKarolinalastNameKnu00edu017ekovu00e1creatorTypeauthorfirstNameNikolalastNameMalinsku00e1creatorTypeauthorfirstNameLilianalastNameTuu0161kovu00e1creatorTypeauthorfirstNameMariannelastNameBoescreatorTypeauthorfirstNameJanlastNameu010cernu00fdabstractNoteAbstractTheMHCIIEGFPknock-inmousemodelenablesustovisualizeandtrackMHC-II-expressingcellsinvivobyexpressingenhancedgreenfluorescentproteinEGFPfusedtotheMHCclassIImoleculeundertheMHCIIbetachainpromoter.UsingthismodelwecaneasilyidentifyMHC-II-expressingcellsincludingdendriticcellsBcellsmacrophagesandILC3swhichplayakeyroleasantigen-presentingcellsAPCsforCD4Tcells.InadditionwecanalsopreciselyidentifyandanalyzeAPC-containingtissuesandorgans.EvenafterfixationEGFPretainsitsfluorescencesothismodelissuitableforimmunofluorescencestudiesfacilitatinganunbiasedcharacterizationofthehistologicalcontextespeciallywithtechniquessuchaslight-sheetfluorescencemicroscopy.FurthermoretheMHCIIEGFPknock-inmousemodelisvaluableforstudyingthemolecularmechanismsofMHCIIgeneregulationandexpressionbymakingitpossibletocorrelateMHCIIexpressionMHCIIEGFPwithsurfacefractionthroughantibodydetectiontherebysheddinglightontheintricateregulationofMHCIIexpression.OverallthismodelisanessentialassetforquantitativeandsystemsimmunologicalresearchprovidinginsightsintoimmunecelldynamicsandlocalizationwithatoolforprecisecellidentificationandwiththeabilitytostudyMHCIIgeneregulationthusfurtheringtheunderstandingofimmuneresponsesandunderlyingmechanisms2023TheAuthors.CurrentProtocolspublishedbyWileyPeriodicalsLLC.BasicProtocol1Characterizationofantigen-specificMHCIIloadingcompartmenttubulationtowardtheimmunologicalsynapseBasicProtocol2CharacterizationofoverallversussurfaceMHCIIexpressionBasicProtocol3IdentificationandpreparationofthelymphoidorgansBasicProtocol4QuantificationofAPCcontentinlymphoidorgansbyfluorescencestereomicroscopyBasicProtocol5Quantificationandmeasurementofintestinallymphoidtissuebylight-sheetfluorescencestereomicroscopyBasicProtocol6VisualizationofcornealAPCsBasicProtocol7QuantificationofMHCIIcellsinmaternalmilkbyflowcytometrySupportProtocol1Cellsurfacestainingandflowcytometryanalysisofspleenmononuclearcellsdate2023-11sectionpartNumberpartTitleDOIhttpsdoi.org10.1002cpz1.925citationKeyurlhttpsdoi.org10.1002cpz1.925PMIDPMCIDISSNlanguagecollectionsMPJ5STUGdateModified2025-03-19T115833ZkeyXJR724QJlibraryid5891878metacreatorSummaryNemcovaetal.parsedDate2023-10-25numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtNemcovaY.FaturovaJ.x160kaloudP.ltbgtComparingContinentalandLocalDistributionPatternsofProtistsACaseStudyofSilica-ScaledChrysophytesltbgt.ltigtFottealtigtltbgt2023ltbgtltigt23ltigt2177x2013189.ltaclass039zp-ItemURL039href039httpsdoi.org10.5507fot.2022.022039gthttpsdoi.org10.5507fot.2022.022ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleComparingcontinentalandlocaldistributionpatternsofprotistsAcasestudyofsilica-scaledchrysophytescreatorscreatorTypeauthorfirstNameYvonnelastNameNemcovacreatorTypeauthorfirstNameJanalastNameFaturovacreatorTypeauthorfirstNamePavellastNameu0160kaloudabstractNoteYvonneNemcovaJanaFaturovaPavelu0160kalouddate20231025sectionpartNumberpartTitleDOI10.5507fot.2022.022citationKeyurlhttpsdoi.org10.5507fot.2022.022PMIDPMCIDISSN1802543918054927languageencollectionsMPJ5STUGdateModified2025-11-07T085306Zkey2VEUTX8Clibraryid5891878metacreatorSummaryPusztaietal.parsedDate2023-10-25numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtPusztaiM.JadrnxE1I.x160kaloudP.ltbgtElucidatingthePhylogenyandTaxonomicPositionoftheGenusSpiniferomonasTakahashiChrysophyceaeltbgt.ltigtFottealtigtltbgt2023ltbgtltigt23ltigt2217x2013222.ltaclass039zp-ItemURL039href039httpsdoi.org10.5507fot.2023.006039gthttpsdoi.org10.5507fot.2023.006ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleElucidatingthephylogenyandtaxonomicpositionofthegenusSpiniferomonasTakahashiChrysophyceaecreatorscreatorTypeauthorfirstNameMartinlastNamePusztaicreatorTypeauthorfirstNameIvalastNameJadrnu00e1creatorTypeauthorfirstNamePavellastNameu0160kaloudabstractNoteMartinPusztaiIvaJadrnu00e1Pavelu0160kalouddate20231025sectionpartNumberpartTitleDOI10.5507fot.2023.006citationKeyurlhttpsdoi.org10.5507fot.2023.006PMIDPMCIDISSN1802543918054927languageencollectionsMPJ5STUGdateModified2025-11-07T090122ZkeyBB3A7DM9libraryid5891878metacreatorSummaryKnotekandu0160kaloudparsedDate2023-10-25numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKnotekP.x160kaloudP.ltbgtTheEffectofPatternedStructuresontheMechanicalResistanceofMicroscopicSilicaScalesltbgt.ltigtFottealtigtltbgt2023ltbgtltigt23ltigt2190x2013200.ltaclass039zp-ItemURL039href039httpsdoi.org10.5507fot.2023.007039gthttpsdoi.org10.5507fot.2023.007ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTheeffectofpatternedstructuresonthemechanicalresistanceofmicroscopicsilicascalescreatorscreatorTypeauthorfirstNamePetrlastNameKnotekcreatorTypeauthorfirstNamePavellastNameu0160kaloudabstractNotePetrKnotekPavelu0160kalouddate20231025sectionpartNumberpartTitleDOI10.5507fot.2023.007citationKeyurlhttpsdoi.org10.5507fot.2023.007PMIDPMCIDISSN1802543918054927languageencollectionsMPJ5STUGdateModified2025-11-07T090225Zkey9IDV3D33libraryid5891878metacreatorSummaryPomahacetal.parsedDate2023-05-12numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtPomahacO.Mendez-SanchezD.PolakovaK.MuellerM.SolitoM.-M.BourlandW.A.CepickaI.ltbgtRediscoveryofRemarkablyRareAnaerobicTentaculiferousCiliateGeneraLegendreaandDactylochlamysCiliophoraLitostomatealtbgt.ltigtBiology-Baselltigtltbgt2023ltbgtltigt12ltigt5707.ltaclass039zp-DOIURL039href039httpsdoi.org10.3390biology12050707039gthttpsdoi.org10.3390biology12050707ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleRediscoveryofRemarkablyRareAnaerobicTentaculiferousCiliateGeneraLegendreaandDactylochlamysCiliophoraLitostomateacreatorscreatorTypeauthorfirstNameOndrejlastNamePomahaccreatorTypeauthorfirstNameDaniellastNameMendez-SanchezcreatorTypeauthorfirstNameKaterinalastNamePolakovacreatorTypeauthorfirstNameMichaellastNameMuellercreatorTypeauthorfirstNameMichel-MarielastNameSolitocreatorTypeauthorfirstNameWilliamA.lastNameBourlandcreatorTypeauthorfirstNameIvanlastNameCepickaabstractNoteFree-livinganaerobicciliatesareofconsiderableinterestfromanecologicalandanevolutionarystandpoint.Extraordinarytentacle-bearingpredatorylineageshaveevolvedindependentlyseveraltimeswithinthephylumCiliophoraincludingtworarelyencounteredanaerobiclitostomateangeneraLegendreaandDactylochlamys.Inthisstudywesignificantlyextendthemorphologicalandphylogeneticcharacterizationofthesetwopoorlyknowngroupsofpredatoryciliates.WeprovidethefirstphylogeneticanalysisofthemonotypicgenusDactylochlamysandthethreevalidspeciesofLegendreabasedonthe18SrRNAgeneandITS-28SrRNAgenesequences.Priortothisstudyneithergrouphadbeenstudiedusingsilverimpregnationmethods.Weprovidethefirstprotargol-stainedmaterialandalsoauniquevideomaterialincludingdocumentationforthefirsttimeofthehuntingandfeedingbehaviorofaLegendreaspecies.Webrieflydiscusstheidentityofmethanogenicarchaealandbacterialendosymbiontsofbothgenerabasedon16SrRNAgenesequencesandtheimportanceofcitizenscienceforciliatologyfromahistoricalandcontemporaryperspective.dateMAY122023sectionpartNumberpartTitleDOI10.3390biology12050707citationKeyurlhttpswww.webofscience.comapigatewayGWVersion2SrcAuthGetFTRSrcAppWOSDestURLhttps3A2F2Fct.prod.getft.io2FY2xhcml2YXRlLHVuZGVmaW5lZCxodHRwczovL2RvaS5vcmcvMTAuMzM5MC9iaW9sb2d5MTIwNTA3MDc.MBpZemqshsnbGMyqI6Y7m6dma3AKK9aIwIDFncJ0KVsDestAppGetFTRSrcItemIdWOS000995675500001SrcAppSIDEUW1ED0AD5l35ynCpYiKbmzlvxhzSHMACnH2B9zypD0gkILy3b6M2FDkHeT02BCCTr2B01HeMa47wTe03DPMIDPMCIDISSN2079-7737languageEnglishcollectionsMPJ5STUGdateModified2025-11-07T091659Zkey4RBEBZMTlibraryid5891878metacreatorSummaryDostu00e1letal.parsedDate2023-05numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtDostxE1lV.HumhalovxE1T.BerxE1nkovxE1P.PxE1caltO.LibusovxE1L.ltbgtSWIPMediatesRetromer-IndependentMembraneRecruitmentoftheWASHComplexltbgt.ltigtTrafficltigtltbgt2023ltbgtltigt24ltigt5216x2013230.httpsdoi.orgltaclass039zp-ItemURL039href039httpsdoi.org10.1111tra.12884039gthttpsdoi.org10.1111tra.12884ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleSWIPmediatesretromer-independentmembranerecruitmentoftheWASHcomplexcreatorscreatorTypeauthorfirstNameVojtu011bchlastNameDostu00e1lcreatorTypeauthorfirstNameTerezalastNameHumhalovu00e1creatorTypeauthorfirstNamePavlalastNameBeru00e1nkovu00e1creatorTypeauthorfirstNameOndu0159ejlastNamePu00e1caltcreatorTypeauthorfirstNameLenkalastNameLibusovu00e1abstractNoteAbstractThepentamericWASHcomplexfacilitatesendosomalproteinsortingbyactivatingArp23whichinturnleadstotheformationofF-actinpatchesspecificallyontheendosomalsurface.ItisgenerallyacceptedthatWASHcomplexattachestotheendosomalmembraneviatheinteractionofitssubunitFAM21withtheretromersubunitVPS35.HoweverweobservetheWASHcomplexandF-actinpresentonendosomesevenintheabsenceofVPS35.WeshowthattheWASHcomplexbindstotheendosomalsurfaceinbotharetromer-dependentandaretromer-independentmanner.Theretromer-independentmembraneanchorisdirectlymediatedbythesubunitSWIP.FurthermoreSWIPcaninteractwithanumberofphosphoinositidespecies.Ofthoseourdatasuggestthattheinteractionwithphosphatidylinositol-35-bisphosphatePI35P2iscrucialtotheendosomalbindingofSWIP.OverallthisstudyrevealsanewroleoftheWASHcomplexsubunitSWIPandhighlightstheWASHcomplexasanindependentself-sufficienttraffickingregulator.date2023-05sectionpartNumberpartTitleDOIhttpsdoi.org10.1111tra.12884citationKeyurlhttpsdoi.org10.1111tra.12884PMIDPMCIDISSN1398-9219languagecollectionsMPJ5STUGdateModified2025-03-19T115833ZkeyMH29VXC9libraryid5891878metacreatorSummaryAumontetal.parsedDate2023-03-01numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtAumontC.BerxE1nkovxE1T.McMahonD.P.RadekR.AkamaP.D.Sillam-DussxE8sD.x160obotnxEDkJ.ltbgtTheUltrastructureoftheRostralGlandinSoldiersofltigtVerrucositermesTuberosusltigtBlattodeaTermitidaeNasutitermitinaeltbgt.ltigtArthropodStructureampDevelopmentltigtltbgt2023ltbgtltigt73ltigt101238.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.asd.2023.101238039gthttpsdoi.org10.1016j.asd.2023.101238ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTheultrastructureoftherostralglandinsoldiersofiVerrucositermestuberosusiBlattodeaTermitidaeNasutitermitinaecreatorscreatorTypeauthorfirstNameCu00e9driclastNameAumontcreatorTypeauthorfirstNameTerezalastNameBeru00e1nkovu00e1creatorTypeauthorfirstNameDinoP.lastNameMcMahoncreatorTypeauthorfirstNameRenatelastNameRadekcreatorTypeauthorfirstNamePierreD.lastNameAkamacreatorTypeauthorfirstNameDavidlastNameSillam-Dussu00e8screatorTypeauthorfirstNameJanlastNameu0160obotnu00edkabstractNoteThesoil-feedinghabitisanevolutionarynoveltyfoundinsomeadvancedgroupsoftermites.Thestudyofsuchgroupsisimportanttorevealinginterestingadaptationstothisway-of-life.ThegenusVerrucositermesisonesuchexamplecharacterizedbypeculiaroutgrowthsontheheadcapsuleantennaeandmaxillarypalpswhicharenotfoundinanyothertermite.Thesestructureshavebeenhypothesizedtobelinkedtothepresenceofanewexocrineorgantherostralglandwhosestructurehasremainedunexplored.WehavethusstudiedtheultrastructureoftheepidermallayeroftheheadcapsuleofVerrucositermestuberosussoldiers.Wedescribetheultrastructureoftherostralglandwhichconsistsofclass3secretorycellsonly.ThedominantsecretoryorganellescompriseroughendoplasmicreticulumandGolgiapparatuswhichprovidesecretionsdeliveredtothesurfaceoftheheadlikelymadeofpeptide-derivedcomponentsofunclearfunction.Wediscussapossibleroleoftherostralglandofsoldiersasanadaptationtothefrequentencounterwithsoilpathogensduringsearchfornewfoodresources.date2023-03-01sectionpartNumberpartTitleDOI10.1016j.asd.2023.101238citationKeyurlhttpswww.sciencedirect.comsciencearticlepiiS1467803923000051PMIDPMCIDISSN1467-8039languagecollectionsMPJ5STUGdateModified2025-11-07T091404ZkeyG6KT4PDFlibraryid5891878metacreatorSummaryRu016fu017eiu010dkaetal.parsedDate2023-02-23numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtRx16Fx17Eix10DkaJ.JakubecP.MahlerovxE1K.x160xEDpkovxE1H.NishikawaM.ltbgtIntegrativeTaxonomyandSpeciesDistributionModelsoftheGenusDiamesusHope1840ColeopteraStaphylinidaeSilphinaeltbgt.ltigtSciRepltigtltbgt2023ltbgtltigt13ltigt13192.ltaclass039zp-DOIURL039href039httpsdoi.org10.1038s41598-023-30019-x039gthttpsdoi.org10.1038s41598-023-30019-xltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleIntegrativetaxonomyandspeciesdistributionmodelsofthegenusDiamesusHope1840ColeopteraStaphylinidaeSilphinaecreatorscreatorTypeauthorfirstNameJanlastNameRu016fu017eiu010dkacreatorTypeauthorfirstNamePavellastNameJakubeccreatorTypeauthorfirstNameKarolinalastNameMahlerovu00e1creatorTypeauthorfirstNameHanalastNameu0160u00edpkovu00e1creatorTypeauthorfirstNameMasaakilastNameNishikawaabstractNoteIntegrativetaxonomyofDiamesusHope1840ColeopteraSilphinaeispresented.AdultsofD.bimaculatusPortevin1914endemictoTaiwanandD.osculansVigors1825widelydistributedfromnorthernIndiatoAustraliaareredescribedkeyedandfiguredincludingcharactersofthemaleandfemalegenitaliaofbothspecies.VariationinelytralmaculationinD.osculansisdiscussedandillustrated.TheabsenceofdiagnosticdifferencesofD.osculansvar.reductusPic1917fromD.osculansisdiscussedandtheformernameisconfirmedasajuniorsubjectivesynonymofD.osculans.TypesofallthreenamesavailablewerestudiedalectotypeandparalectotypesaredesignatedforthenameD.osculansvar.bimaculatusPortevin1914.MolecularphylogeneticanalysisconfirmsthegenusDiamesusissistergrouptothegenusNecrodesLeach1815andD.osculansandD.bimaculatusaretwowellsupportedclades.DetaileddataonthedistributionofD.bimaculatusandD.osculansarepresentedandmapped.Speciesdistributionmodelsforbothspecieswerecreatedandinterpreted.DiamesusosculansisreportedforthefirsttimefromIndiaUttarakhandChinaAnhuiHainanHunanJiangxiShaanxiandZhejiangProvincesandAustraliaVictoriaitisalsorecentlyconfirmedfromTaiwanbeingsympatricindistributiontherewithD.bimaculatus.AvailabledataontheecologyandseasonalityofbothspeciesofDiamesusarealsodiscussed.date2023-02-23sectionpartNumberpartTitleDOI10.1038s41598-023-30019-xcitationKeyurlhttpswww.nature.comarticless41598-023-30019-xPMIDPMCIDISSN2045-2322languageencollectionsMPJ5STUGdateModified2025-11-07T085414ZkeyZ8WENF3Zlibraryid5891878metacreatorSummaryShewaleetal.parsedDate2023numChildren3bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtShewaleM.K.NebesxE1x159ovxE1J.Grosse-WildeE.KalinovxE1B.ltbgtMicroscopicMorphologyandDistributionoftheAntennalSensillaintheDouble-SpinedBarkBeetleIpsDuplicatusColeopteraCurculionidaeltbgt.ltigtMicroscopyResearchandTechniqueltigtltbgt2023ltbgtltigt86ltigt121610x20131625.ltaclass039zp-DOIURL039href039httpsdoi.org10.1002jemt.24397039gthttpsdoi.org10.1002jemt.24397ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMicroscopicmorphologyanddistributionoftheantennalsensillainthedouble-spinedbarkbeetleIpsduplicatusColeopteraCurculionidaecreatorscreatorTypeauthorfirstNameMayuriKashinathlastNameShewalecreatorTypeauthorfirstNameJanalastNameNebesu00e1u0159ovu00e1creatorTypeauthorfirstNameEwaldlastNameGrosse-WildecreatorTypeauthorfirstNameBlankalastNameKalinovu00e1abstractNoteThedouble-spinedsprucebarkbeetleIpsduplicatushasbecomeaninfamoussecondarypestofNorwaysprucecausingextensiveecologicalandeconomicdestructioninmanyCentralEuropeancountries.Antennaearetheprimaryolfactoryorgansthatplayafundamentalroleininsect-hostchemicalcommunicationthereforeunderstandingmorphologyiscrucialbeforeconductingelectrophysiologicalinvestigations.HerewepresentouranalysisofsensillatypesontheantennalsurfaceofI.duplicatusforthefirsttimeusinghigh-resolution-scanningelectronmicroscopy.Westudiedtheexternalmorphologicalcharacteristicsofantennaeandthetypesnumbersanddistributionoftheantennalsensillainmalesandfemales.OurresultsrevealedthepresenceoffivedifferenttypesofmorphologicallydistinctsensillasensillachaeticasensillabasiconicasensillatrichodeasensillacoeloconicaandBu00f6hm039ssensilla.WeobservedtwosubtypesofsensillachaeticaSChIandSChIIfoursubtypesofsensillabasiconicaSBISBIISBIIIandSBIVthreesubtypesofsensillatrichodeaSTrIISTrIIIandSTrIVandtwosubtypesofsensillacoeloconicaSCoIandSCoIIrespectivelyinI.duplicatusmalesandfemales.Minordifferencesinlengthandnumbersbetweenthesexesforsomesensillatypeswerefound.Distributionmapsfordifferentsensillartypeswereconstructedandspecificareasfortherespectivesensillawerefound.Possiblefunctionsofobservedsensillatypesarediscussed.ThepresentstudyprovidesabasisforfutureelectrophysiologicalstudiestounderstandhowI.duplicatusdetectsecologicallyimportantolfactorycues.ResearchHighlightsu2022ThefirstreportofmorphologyanddistributionpatternoftheantennalsensillainIpsduplicatusisdiscussed.u2022Atotalof6maintypesand11antennalsensillasubtypeswereobservedinmaleandfemaleIpsduplicatus.u2022Minorsex-specificdifferenceswereseeninthelengthandnumbersinseveralsensillatypes.date2023sectionpartNumberpartTitleDOI10.1002jemt.24397citationKeyurlhttpsonlinelibrary.wiley.comdoiabs10.1002jemt.24397PMIDPMCIDISSN1097-0029languageencollectionsMPJ5STUGdateModified2026-04-20T065426ZkeyMC8V9QI8libraryid5891878metacreatorSummaryMu00e9ndez-Su00e1nchezetal.parsedDate2023numChildren3bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMxE9ndez-SxE1nchezD.Pomahax10DO.RotterovxE1J.BourlandW.A.x10Cepix10DkaI.ltbgtMorphologyandPhylogeneticPositionofThreeAnaerobicCiliatesfromtheClassesOdontostomateaandMuranotricheaCiliophoraltbgt.ltigtJournalofEukaryoticMicrobiologyltigtltbgt2023ltbgtltigt70ltigt3e12965.ltaclass039zp-DOIURL039href039httpsdoi.org10.1111jeu.12965039gthttpsdoi.org10.1111jeu.12965ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMorphologyandphylogeneticpositionofthreeanaerobicciliatesfromtheclassesOdontostomateaandMuranotricheaCiliophoracreatorscreatorTypeauthorfirstNameDaniellastNameMu00e9ndez-Su00e1nchezcreatorTypeauthorfirstNameOndu0159ejlastNamePomahau010dcreatorTypeauthorfirstNameJohanalastNameRotterovu00e1creatorTypeauthorfirstNameWilliamA.lastNameBourlandcreatorTypeauthorfirstNameIvanlastNameu010cepiu010dkaabstractNoteThediversityoftheclassesOdontostomateaandMuranotricheawhichcontainsolelyobligateanaerobesispoorlyunderstood.WestudiedtwopopulationsofMylestomasp.oneofSaprodiniumdentatumOdontostomateatwoofMuranothrixfelixsp.nov.andoneofMuranothrixsp.Muranotricheaemployingliveobservationprotargolimpregnationscanningelectronmicroscopyand18SrRNAgenesequencing.ConspecificityofMylestomasp.describedherewithapreviouslydescribedspeciesofthisgenuscannotbeexcludedsincenospecieshavebeenstudiedwithmodernmethods.PhylogeneticallythegenusMylestomaiscloselyrelatedtotheodontostomatidDiscomorphellapedroeneasialthoughthephylogeneticpositionofclassOdontostomateaitselfremainsunresolved.ThenewlydescribedmuranotricheanspeciesMuranothrixfelixsp.nov.ismorphologicallysimilartoM.gubernatabutcanbedistinguishedbyitsfewermacronuclearnodulesandfeweradoralmembranellesmoreoveritisclearlydistinguishedfromM.gubernatabyits18SrRNAgenesequence.AnotherpopulationdesignatedhereasMuranothrixsp.mostlikelyrepresentsaseparatespecies.date2023sectionpartNumberpartTitleDOI10.1111jeu.12965citationKeyurlhttpsonlinelibrary.wiley.comdoiabs10.1111jeu.12965PMIDPMCIDISSN1550-7408languageencollectionsMPJ5STUGdateModified2025-11-07T085611Zkey62FZ9J3Vlibraryid5891878metacreatorSummaryStencetal.parsedDate2023numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtStencJ.JanosxEDkL.MatouskovxE1E.HadravaJ.MikxE1tM.JanovskyZ.ltbgtPollinatorVisitationCloselyTracksDiurnalPatternsinPollenReleaseltbgt.ltigtAMERICANJOURNALOFBOTANYltigtltbgt2023ltbgtltigt110ltigt6.ltaclass039zp-DOIURL039href039httpsdoi.org10.1002ajb2.16179039gthttpsdoi.org10.1002ajb2.16179ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitlePollinatorvisitationcloselytracksdiurnalpatternsinpollenreleasecreatorscreatorTypeauthorfirstNameJ.lastNameStenccreatorTypeauthorfirstNameL.lastNameJanosu00edkcreatorTypeauthorfirstNameE.lastNameMatouskovu00e1creatorTypeauthorfirstNameJ.lastNameHadravacreatorTypeauthorfirstNameM.lastNameMiku00e1tcreatorTypeauthorfirstNameZ.lastNameJanovskyabstractNotePremiseAnimal-pollinatedplantsfaceahighriskofpollenlossduringitstransfer.Tolimitthenegativeeffectofpollenlossesbypollenconsumptionandheterospecifictransferplantspeciesmayadjustandstratifytheirpollenavailabilityduringthedayi.e.quotschedulequottheirpollenpresentationandattractpollinatorsinspecifictimeframes.MethodsWeinvestigateddiurnalpatternsofpollenavailabilityandpollinatorvisitationinthreecofloweringplantspeciesSuccisapratensiswithopenflowersandaccessiblepollenpollinatedmainlybypollen-feedinghoverfliesCentaureajaceawithopenflowersandlessaccessiblepollenpollinatedmainlybypollen-collectingbeesandTrifoliumhybridumwithclosedflowersandpollenaccessibleonlyaftertheactiveopeningoftheflowerpollinatedexclusivelybybees.ResultsThethreeplantspeciesdifferedinthepeakpollenavailabilitytrackedbythevisitationactivityoftheirpollinators.Succisapratensisreleasedallpolleninthemorningwhilepollinatoractivitywasstilllowandpeakedwithaslightdelay.IncontrastC.jaceaandT.hybridumhaddistinctpollenpresentationschedulespeakingintheearlyafternoon.Thepollinatorvisitationtobothofthesespeciescloselymatchedtheirpollenavailability.ConclusionsStratifyingpollenavailabilitytopollinatorsduringthedaymaybeoneofseveralmechanismsthatallowcofloweringplantstosharetheirpollinatorsanddecreasetheprobabilityofheterospecificpollentransfer.date2023sectionpartNumberpartTitleDOI10.1002ajb2.16179citationKeyurlPMIDPMCIDISSN0002-9122languagecollectionsMPJ5STUGdateModified2025-03-19T115836ZkeySIZZX3XVlibraryid5891878metacreatorSummaryPolu00e1kovu00e1etal.parsedDate2023numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtPolxE1kovxE1K.BourlandW.A.CepickaI.ltbgtAnaerocyclidiidaeFam.Nov.OligohymenophoreaScuticociliatiaANewlyRecognizedMajorLineageofAnaerobicCiliatesHostingProkaryoticSymbiontsltbgt.ltigtEUROPEANJOURNALOFPROTISTOLOGYltigtltbgt2023ltbgtltigt90ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.ejop.2023.126009039gthttpsdoi.org10.1016j.ejop.2023.126009ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleAnaerocyclidiidaefam.nov.OligohymenophoreaScuticociliatiaAnewlyrecognizedmajorlineageofanaerobicciliateshostingprokaryoticsymbiontscreatorscreatorTypeauthorfirstNameK.lastNamePolu00e1kovu00e1creatorTypeauthorfirstNameW.A.lastNameBourlandcreatorTypeauthorfirstNameI.lastNameCepickaabstractNoteTheresearchonanaerobicciliatestodatehasmainlybeenfocusedonrepresentativesofobligatelyanaerobicclassessuchasArmophoreaorPlagiopylea.InthisstudywefocusontheanaerobicrepresentativesofthesubclassScuticociliatiamembersoftheclassOligohymenophoreawhichismainlycomposedofaerobicciliates.UntilnowonlyasingleanaerobicspeciesCyclidiumporcatumheretransferredtothegenusAnaerocyclidiumgen.nov.hasbeendescribedbothmolecularlyandmorphologically.Ourbroadsamplingofanoxicsedimentstogetherwithcultivationandsinglecellsequencingapproacheshaveshownthatscuticociliatesarecommonanddiversifiedinanoxicenvironments.OurresultsshowthatanaerobicscuticociliatesrepresentadistinctiveevolutionarylineagenotcloselyrelatedtothefamilyCyclidiidaeorderPleuronematidaaspreviouslysuggested.HoweverthephylogeneticpositionofthenewlyrecognizedlineagewithinthesubclassScuticociliatiaremainsunresolved.BasedonmolecularandmorphologicaldataweestablishthefamilyAnaerocyclidiidaefam.nov.toaccommodatemembersofthisclade.Wefurtherprovidedetailedmorphologicaldescriptionsand18SrRNAgenesequencesforsixnewAnaerocyclidiumspeciesandsignificantlybroadenthedescribeddiversityofanaerobicscuticociliates.date2023sectionpartNumberpartTitleDOI10.1016j.ejop.2023.126009citationKeyurlPMIDPMCIDISSN0932-4739languagecollectionsMPJ5STUGdateModified2025-03-19T115836ZkeyFNU7CPLXlibraryid5891878metacreatorSummaryBudiletal.parsedDate2023numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtBudilJ.StenclovxE1P.KromkaA.LiskovxE1P.ltbgtDevelopmentoftheltigtPseudomonasSyringaeltigtPv.ltigtMorsprunorumltigtBiofilmMonitoredinRealTimeUsingAttenuatedTotalReflectionFourierTransformInfraredMeasurementsinaFlowCellChamberltbgt.ltigtAPPLIEDSPECTROSCOPYltigtltbgt2023ltbgtltigt77ltigt5500x2013512.ltaclass039zp-DOIURL039href039httpsdoi.org10.117700037028231165057039gthttpsdoi.org10.117700037028231165057ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDevelopmentoftheiPseudomonassyringaeipv.imorsprunorumiBiofilmMonitoredinRealTimeUsingAttenuatedTotalReflectionFourierTransformInfraredMeasurementsinaFlowCellChambercreatorscreatorTypeauthorfirstNameJ.lastNameBudilcreatorTypeauthorfirstNameP.lastNameStenclovu00e1creatorTypeauthorfirstNameA.lastNameKromkacreatorTypeauthorfirstNameP.lastNameLiskovu00e1abstractNoteBiofilmsofsessilePseudomonassyringaecellsformedontopofplanthost039sleavesorfruitsallowsurvivingharshenvironmentalconditionsdesiccationandimprovetheirresistancetoantibacterialtreatmentsofcrops.Abetterunderstandingofthesebiofilmscanhelpminimizetheireffectonharvests.InthepresentstudyinfraredattenuatedtotalreflectionspectroscopycoupledwithopticalandconfocallaserscanningmicroscopyhasbeenappliedforthefirsttimetoanalyzePseudomonassyringaepathovarmorsprunorumbiofilmdevelopmentinrealtime.Thebiofilmdevelopmentwasobservedwithinaspectralwindow4000-800cm-1underconstantflowconditionsfor72h.Thekineticsofrepresentativeintegratedbandareasnucleicacidswithpolysaccharidesat1141-1006cm-1aminoacidsidechainswithfreefattyacidsat1420-1380cm-1proteinsat1580-1490cm-1andlipidswithproteinsat2935-2915cm-1wereanalyzedwithregardtotheobservedbiofilmstructureandthefollowingP.syringaebiofilmdevelopmentalstageswereattributedTheinoculationphasewashingofweaklyattachedbacteriacloselyfollowedbyrecolonizationofthevacatedsurfacetherestructurationphaseandfinallythematurationphase.date2023sectionpartNumberpartTitleDOI10.117700037028231165057citationKeyurlPMIDPMCIDISSN0003-7028languagecollectionsMPJ5STUGdateModified2025-03-19T115836ZkeyWV4MSL3Elibraryid5891878metacreatorSummaryHuberetal.parsedDate2023numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHuberB.A.MengG.L.KrxE1lJ.HerreraI.M.A.IzquierdoM.A.CarvalhoL.S.ltbgtHighandDryIntegrativeTaxonomyoftheAndeanSpiderGenusNerudiaAraneaePholcidaeltbgt.ltigtZOOLOGICALJOURNALOFTHELINNEANSOCIETYltigtltbgt2023ltbgtltigt198ltigt2534x2013591.ltaclass039zp-DOIURL039href039httpsdoi.org10.1093zoolinneanzlac100039gthttpsdoi.org10.1093zoolinneanzlac100ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleHighanddryintegrativetaxonomyoftheAndeanspidergenusNerudiaAraneaePholcidaecreatorscreatorTypeauthorfirstNameB.A.lastNameHubercreatorTypeauthorfirstNameG.L.lastNameMengcreatorTypeauthorfirstNameJ.lastNameKru00e1lcreatorTypeauthorfirstNameI.M.A.lastNameHerreracreatorTypeauthorfirstNameM.A.lastNameIzquierdocreatorTypeauthorfirstNameL.S.lastNameCarvalhoabstractNoteNinetinaeareagroupofpoorlyknownspidersthatdonotfittheimageof039daddylong-legsspiders039Pholcidaethefamilytowhichtheybelong.Theyaremostlyshort-leggedtinyandliveinaridenvironments.ThepreviouslymonotypicAndeangenusNerudiaexemplifiesourpoorknowledgeofNinetinaeonlysevenadultspecimensfromtwolocalitiesinChileandArgentinahavebeenreportedintheliterature.WefoundrepresentativesofNerudiaat24of52localitiesvisitedin2019mostlyunderrocksinaridhabitatsupto4450ma.s.l.thehighestknownrecordforPholcidae.Withnowmorethan400adultspecimenswerevisethegenusdescribingtennewspeciesbasedonmorphologyincludingSEMandCOIbarcodes.WepresentthefirstkaryotypedataforNerudiaandforitsputativesister-genusGertschiola.ThesetwosouthernSouthAmericangenerashareaX1X2X3Ysexchromosomesystem.WemodelthedistributionofNerudiashowingthatthegenusisexpectedtooccurintheAtacamabiogeographicprovincenorecordsofarandthatitsenvironmentalnicheisphylogeneticallyconserved.ThisisthefirstcomprehensiverevisionofanyNinetinaegenus.Itsuggeststhatfocusedcollectingmayuncoveraconsiderablediversityoftheseenigmaticspiders.date2023sectionpartNumberpartTitleDOI10.1093zoolinneanzlac100citationKeyurlPMIDPMCIDISSN0024-4082languagecollectionsMPJ5STUGdateModified2025-03-19T115835ZkeyDPRBZJ28libraryid5891878metacreatorSummaryMajeretal.parsedDate2023numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMajerJ.KindermannM.PinkasD.ChvatilD.CiglerP.LibusovaL.ltbgtCellularUptakeandFateofCationicPolymer-CoatedNanodiamondsDeliveringsiRNAAMechanisticStudyltbgt.ltigtNANOSCALEltigtltbgt2023ltbgt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1039d3nr05738k039gthttpsdoi.org10.1039d3nr05738kltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleCellularuptakeandfateofcationicpolymer-coatednanodiamondsdeliveringsiRNAamechanisticstudycreatorscreatorTypeauthorfirstNameJ.lastNameMajercreatorTypeauthorfirstNameM.lastNameKindermanncreatorTypeauthorfirstNameD.lastNamePinkascreatorTypeauthorfirstNameD.lastNameChvatilcreatorTypeauthorfirstNameP.lastNameCiglercreatorTypeauthorfirstNameL.lastNameLibusovaabstractNoteGenesilencingusingsmallinterferingRNAssiRNAsisaselectiveandpromisingapproachfortreatmentofnumerousdiseases.HoweverbroadapplicationsofsiRNAsarecompromisedbytheirlowstabilityinabiologicalenvironmentandlimitedabilitytopenetratecells.NanodiamondsNDscoatedwithcationicpolymerscanenablecellulardeliveryofsiRNAs.RecentlywedevelopedanewtypeofNDcoatingbasedonarandomcopolymerconsistingof2-dimethylaminoethylmethacrylateDMAEMAandN-2-hydroxypropylmethacrylamideHPMAmonomers.ThesehybridND-polymerparticlesCop-FNDprovidenear-infraredfluorescenceformstablecomplexeswithsiRNAinserumshowlowtoxicityandeffectivelydeliversiRNAintocellsinvitroandinvivo.HerewepresentdataonthemechanismofcellularuptakeandcelltraffickingofCop-FNDsiRNAcomplexesandtheirabilitytoselectivelysuppressmRNAlevelsaswellastheircytotoxicityviabilityandcolloidalstability.Weidentifiedclathrin-mediatedendocytosisasthepredominantentrymechanismforCop-FNDsiRNAintoU-2OShumanboneosteosarcomacellswithasubstantialfractionofCop-FNDsiRNAfollowingthelysosomepathway.Cop-FNDsiRNApotentlyinhibitedthetargetGAPDHgenewithnegligibletoxicityandsufficientcolloidalstability.BasedonourresultswesuggestthatCop-FNDsiRNAcanserveasasuitableinvivodeliverysystemforsiRNA.Nanodiamondscoatedwitharandomcationiccopolymerbasedon2-dimethylaminoethylmethacrylateDMAEMAandN-2-hydroxypropylmethacrylamideHPMAenablehighlyeffectivecellulardeliveryofsiRNAs.Clathrin-mediatedendocytosisisthepredominantentrymechanism.date2023sectionpartNumberpartTitleDOI10.1039d3nr05738kcitationKeyurlPMIDPMCIDISSN2040-3364languagecollectionsMPJ5STUGdateModified2025-03-19T115835ZkeyZ398AA9Wlibraryid5891878metacreatorSummaryHuberetal.parsedDate2023numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHuberB.A.MengG.L.KralJ.HerreraI.M.A.IzquierdoM.A.ltbgtRevisionoftheSouthAmericanNinetinaeGenusltigtGuaranitaltigtAraneaePholcidaeltbgt.ltigtEUROPEANJOURNALOFTAXONOMYltigtltbgt2023ltbgtltigt900ltigt32x201380.ltaclass039zp-DOIURL039href039httpsdoi.org10.5852ejt.2023.900.2301039gthttpsdoi.org10.5852ejt.2023.900.2301ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleRevisionoftheSouthAmericanNinetinaegenusiGuaranitaiAraneaePholcidaecreatorscreatorTypeauthorfirstNameB.A.lastNameHubercreatorTypeauthorfirstNameG.L.lastNameMengcreatorTypeauthorfirstNameJ.lastNameKralcreatorTypeauthorfirstNameI.M.AvilalastNameHerreracreatorTypeauthorfirstNameM.A.lastNameIzquierdoabstractNoteThesouthernSouthAmericangenusGuaranitaincludestinyspidersbodylengthsimilarto1mmthatleadreclusivelivesunderground-objectsandrunrapidlywhendisturbed.Asaresulttheyhavebeenpoorlycollectedandstudied.HerewereportonarecentcollectionofGuaranitaspidersfromArgentinadescribingonenewspeciesG.auadaeHubersp.nov.andthepreviouslyunknownfemaleofG.dobbyTorresetal.2016.InadditionweprovideCO1barcodesforallnowfiveknownspeciesfirstSEMdataandfirstchromosomedataforthegenus.ThediploidnumberofGuaranitagoloboffiHuber20002nmale11isamongthelowestinaraneomorphspiderswithmonocentricchromosomestructure.date2023sectionpartNumberpartTitleDOI10.5852ejt.2023.900.2301citationKeyurlPMIDPMCIDISSN2118-9773languagecollectionsMPJ5STUGdateModified2025-03-19T115833Zkey6WWGUMAFlibraryid5891878metacreatorSummaryMazancovu00e1etal.parsedDate2023numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMazancovxE1E.ZadrobilkovxE1E.YubukiN.CepickaI.ltbgtPhylogeneticandMorphologicalDiversityofFree-LivingDiplomonadsltbgt.ltigtEUROPEANJOURNALOFPROTISTOLOGYltigtltbgt2023ltbgtltigt91ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.ejop.2023.126024039gthttpsdoi.org10.1016j.ejop.2023.126024ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitlePhylogeneticandmorphologicaldiversityoffree-livingdiplomonadscreatorscreatorTypeauthorfirstNameE.lastNameMazancovu00e1creatorTypeauthorfirstNameE.lastNameZadrobilkovu00e1creatorTypeauthorfirstNameN.lastNameYubukicreatorTypeauthorfirstNameI.lastNameCepickaabstractNoteDiplomonadidaisalineageofanaerobicprotistsbelongingtoFornicataMetamonada.MostdiplomonadsareendobioticorparasiticsuchasGiardiaintestinaliswhichisafamoushumanpathogenbutseveralfree-livingspeciesexistaswell.Althoughithasbeenproposedthatthefree-livingdiplomonadsaredescendantsofendobioticorganismsandthusinterestingfromtheevolutionarypointofviewtheyhavebeenlargelyneglected.Weobtained58culturesoffree-livingdiplomonadsbelongingtofourgeneraHexamitaTrepomonasGyromonasandTrimitusandsixstrainsofendobioticdiplomonadsandanalyzedtheirSSUrRNAgenesequences.Wealsostudiedlight-microscopicmorphologyofselectedstrainsandtheultrastructureofTrepomonasrotansforthefirsttime.OurphylogeneticanalysisshowedthatthegenusHexamitaandpossiblyalsothegenusTrepomonasarepolyphyletic.TrepomonasrotanswhichmayrepresentanovelgenusisuniqueamongDiplomonadidabyhavingthecellcoveredinscales.Ourresultssuggestthattheevolutionoftheendobioticlifestyleandcellorganizationindiplomonadsismorecomplicatedthanpreviouslythought.date2023sectionpartNumberpartTitleDOI10.1016j.ejop.2023.126024citationKeyurlPMIDPMCIDISSN0932-4739languagecollectionsMPJ5STUGdateModified2025-03-19T115833ZkeyI9R58636libraryid5891878metacreatorSummaryHobothetal.parsedDate2023numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHobothP.SztachoM.QuaasA.AkgxFClB.HozxE1kP.ltbgtQuantitativeSuper-ResolutionMicroscopyRevealstheDifferencesintheNanoscaleDistributionofNuclearPhosphatidylinositol45-BisphosphateinHumanHealthySkinandSkinWartsltbgt.ltigtFRONTIERSINCELLANDDEVELOPMENTALBIOLOGYltigtltbgt2023ltbgtltigt11ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.3389fcell.2023.1217637039gthttpsdoi.org10.3389fcell.2023.1217637ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleQuantitativesuper-resolutionmicroscopyrevealsthedifferencesinthenanoscaledistributionofnuclearphosphatidylinositol45-bisphosphateinhumanhealthyskinandskinwartscreatorscreatorTypeauthorfirstNameP.lastNameHobothcreatorTypeauthorfirstNameM.lastNameSztachocreatorTypeauthorfirstNameA.lastNameQuaascreatorTypeauthorfirstNameB.lastNameAkgu00fclcreatorTypeauthorfirstNameP.lastNameHozu00e1kabstractNoteIntroductionImagingofhumanclinicalformalin-fixedparaffin-embeddedFFPEtissuesectionsprovidesinsightsintohealthyanddiseasedstatesandthereforerepresentsavaluableresourceforbasicresearchaswellasfordiagnosticandclinicalpurposes.Howeverconventionallightmicroscopydoesnotallowtoobservethemoleculardetailsoftissueandcellarchitectureduetothediffractionlimitoflight.Super-resolutionmicroscopyovercomesthislimitationandprovidesaccesstothenanoscaledetailsoftissueandcellorganization.MethodsHereweusedquantitativemulticolorstimulatedemissiondepletionSTEDnanoscopytostudythenanoscaledistributionofthenuclearphosphatidylinositol45-bisphosphatenPI45P2withrespecttothenuclearspecklesNSmarkerSON.ResultsIncreasednPI45P2signalswerepreviouslylinkedtohumanpapillomavirusHPV-mediatedcarcinogenesiswhileNS-associatedPI45P2representsthelargestpoolofnPI45P2visualizedbystainingandmicroscopy.TheimplementationofmulticolorSTEDnanoscopyinhumanclinicalFFPEskinandwartsectionsallowedustoprovideherethequantitativeevidenceforhigherlevelsofNS-associatedPI45P2inHPV-inducedwartscomparedtocontrolskin.DiscussionThesedataexpandthepreviousreportsofHPV-inducedincreaseofnPI45P2levelsandrevealforthefirsttimethefunctionaltissue-specificlocalizationofnPI45P2withinNSinclinicallyrelevantsamples.MoreoverourapproachiswidelyapplicabletootherhumanclinicalFFPEtissuesasaninformativeadditiontotheclassicalhistochemistry.date2023sectionpartNumberpartTitleDOI10.3389fcell.2023.1217637citationKeyurlPMIDPMCIDISSN2296-634XlanguagecollectionsMPJ5STUGdateModified2025-03-19T115833ZkeyYB98T7AAlibraryid5891878metacreatorSummaryMarvanovaetal.parsedDate2023numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMarvanovaA.KasikP.ElsnicovaB.TibenskaV.GalatikF.HornikovaD.ZvolskaV.VebrP.VodickaP.HejnovaL.MatousP.BacovaB.S.SykoraM.NovotnyJ.NeuzilJ.KolarF.NovakovaO.ZurmanovaJ.M.ltbgtContinuousShort-TermAcclimationtoModerateColdElicitsCardioprotectioninRatsandAltersx3B2-AdrenergicSignalingandImmuneStatusltbgt.ltigtScientificReportsltigtltbgt2023ltbgtltigt13ltigt118287.ltaclass039zp-ItemURL039href039httpsdoi.org10.1038s41598-023-44205-4039gthttpsdoi.org10.1038s41598-023-44205-4ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleContinuousshort-termacclimationtomoderatecoldelicitscardioprotectioninratsandaltersu03b2-adrenergicsignalingandimmunestatuscreatorscreatorTypeauthorfirstNameAnetalastNameMarvanovacreatorTypeauthorfirstNamePetrlastNameKasikcreatorTypeauthorfirstNameBarbaralastNameElsnicovacreatorTypeauthorfirstNameVeronikalastNameTibenskacreatorTypeauthorfirstNameFrantiu0161eklastNameGalatikcreatorTypeauthorfirstNameDanielalastNameHornikovacreatorTypeauthorfirstNameVeronikalastNameZvolskacreatorTypeauthorfirstNamePavellastNameVebrcreatorTypeauthorfirstNamePetrlastNameVodickacreatorTypeauthorfirstNameLucielastNameHejnovacreatorTypeauthorfirstNamePetrlastNameMatouscreatorTypeauthorfirstNameBarbaraSzeifflastNameBacovacreatorTypeauthorfirstNameMatuslastNameSykoracreatorTypeauthorfirstNameJirilastNameNovotnycreatorTypeauthorfirstNameJirilastNameNeuzilcreatorTypeauthorfirstNameFrantiseklastNameKolarcreatorTypeauthorfirstNameOlgalastNameNovakovacreatorTypeauthorfirstNameJitkaM.lastNameZurmanovaabstractNoteModeratecoldacclimationMCAisanon-invasiveinterventionmitigatingeffectsofvariouspathologicalconditionsincludingmyocardialinfarction.WeaimtodeterminetheshortestcardioprotectiveregimenofMCAandtheresponseofu03b2123-adrenoceptorsu03b2-ARitsdownstreamsignalingandinflammatorystatuswhichplayaroleincell-survivalduringmyocardialinfarction.AdultmaleWistarratswereacclimated9u00b0C1u20133-10days.InfarctsizeechocardiographywesternblottingELISAmitochondrialrespirometryreceptorbindingassayandquantitativeimmunofluorescencemicroscopywerecarriedoutonleftventricularmyocardiumandbrownadiposetissueBAT.MultiPlexanalysisofcytokinesandchemokinesinserumwasaccomplished.Wefoundthatshort-termMCAreducedmyocardialinfarctionimprovedresistanceofmitochondriatoCa2-overloadanddownregulatedu03b21-ARs.Theu03b22-ARsproteinkinaseBAktwereattenuatedwhileu03b23-ARstranslocatedontheT-tubularsystemsuggestingitsactivation.ProteinkinaseGPKGtranslocatedtosarcoplasmicreticulumandphosphorylationofAMPKThr172increasedafter10days.Principalcomponentanalysisrevealedasignificantshiftincytokinechemokineserumlevelsonday10ofacclimationwhichcorrespondstomaturationofBAT.Inconclusionshort-termMCAincreasesheartresiliencetoischemiawithoutanynegativesideeffectssuchashypertensionorhypertrophy.Cold-elicitedcardioprotectionisaccompaniedbyu03b212-ARdesensitizationactivationoftheu03b23-ARPKGAMPKpathwaysandanimmunomodulatoryeffect.date2023sectionpartNumberpartTitleDOI10.1038s41598-023-44205-4citationKeyurlhttpsdoi.org10.1038s41598-023-44205-4PMIDPMCIDISSN2045-2322languagecollectionsMPJ5STUGdateModified2025-03-19T115833ZkeyN5QIINJJlibraryid5891878metacreatorSummaryMartineketal.parsedDate2023numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMartinekJ.CifrovxE1P.Vosolsobx11BS.GarcxEDa-GonzxE1lezJ.MalxEDnskxE1K.MauerovxE1Z.JelxEDnkovxE1B.KrtkovxE1J.SikorovxE1L.LeavesI.SparkesI.SchwarzerovxE1K.ltbgtARP23ComplexAssociateswithPeroxisomestoParticipateinPexophagyinPlantsltbgt.ltigtNaturePlantsltigtltbgt2023ltbgtltigt9ltigt111874x20131889.ltaclass039zp-ItemURL039href039httpsdoi.org10.1038s41477-023-01542-6039gthttpsdoi.org10.1038s41477-023-01542-6ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleARP23complexassociateswithperoxisomestoparticipateinpexophagyinplantscreatorscreatorTypeauthorfirstNameJanlastNameMartinekcreatorTypeauthorfirstNamePetralastNameCifrovu00e1creatorTypeauthorfirstNameStanislavlastNameVosolsobu011bcreatorTypeauthorfirstNameJudithlastNameGarcu00eda-Gonzu00e1lezcreatorTypeauthorfirstNameKateu0159inalastNameMalu00ednsku00e1creatorTypeauthorfirstNameZdeu0148kalastNameMauerovu00e1creatorTypeauthorfirstNameBarboralastNameJelu00ednkovu00e1creatorTypeauthorfirstNameJanalastNameKrtkovu00e1creatorTypeauthorfirstNameLenkalastNameSikorovu00e1creatorTypeauthorfirstNameIanlastNameLeavescreatorTypeauthorfirstNameImogenlastNameSparkescreatorTypeauthorfirstNameKateu0159inalastNameSchwarzerovu00e1abstractNoteActin-relatedproteinARP23complexisaheteroheptamericproteincomplexevolutionaryconservedinalleukaryoticorganisms.Itsconservedroleisbasedontheinductionofactinpolymerizationattheinterfacebetweenmembranesandthecytoplasm.PlantARP23hasbeenreportedtoparticipateinactinreorganizationattheplasmamembraneduringpolarizedgrowthoftrichomesandattheplasmamembraneu2013endoplasmicreticulumcontactsites.HerewedemonstratethatindividualplantsubunitsofARP23fusedtofluorescentproteinsformmotilespot-likestructuresinthecytoplasmthatareassociatedwithperoxisomesinArabidopsisandtobacco.ARP23isfoundattheperoxisomeperipheryandcontainstheassembledARP23complexandtheWAVESCARcomplexsubunitNAP1.ThisARP23-positiveperoxisomaldomaincolocalizeswiththeautophagosomeandunderconditionsthataffecttheautophagycolocalizationbetweenARP23andtheautophagosomeincreases.ARP23subunitsco-immunoprecipitatewithATG8fandperoxisome-associatedARP23interactinvivowiththeATG8fmarker.SincemutantslackingfunctionalARP23complexhavemoreperoxisomesthanwildtypewesuggestthatARP23hasanovelroleintheprocessofperoxisomedegradationbyautophagycalledpexophagy.date2023sectionpartNumberpartTitleDOI10.1038s41477-023-01542-6citationKeyurlhttpsdoi.org10.1038s41477-023-01542-6PMIDPMCIDISSN2055-0278languagecollectionsMPJ5STUGdateModified2025-03-19T115833Z
1.
Thakur, H.; Agarwal, S.; Hradecký, J.; Sharma, G.; Li, H.-F.; Yang, S.-E.; Sehadová, H.; Chandel, R. S.; Hyliš, M.; Mathur, V.; Šobotník, J.; Sillam-Dussès, D. The Trail-Following Communication in Stylotermes Faveolus and S. Halumicus (Blattodea, Isoptera, Stylotermitidae). J Chem Ecol 2023, 49 (11), 642–651. https://doi.org/10.1007/s10886-023-01447-w.
1.
Nemcova, Y.; Diaz-Pulido, G. Floristic and Ecological Insights into Silica-Scaled Chrysophytes in Southeastern Queensland, Australia. Plant Syst Evol 2023, 309 (6), 43. https://doi.org/10.1007/s00606-023-01881-z.
1.
Pačes, J.; Grobárová, V.; Zadražil, Z.; Knížková, K.; Malinská, N.; Tušková, L.; Boes, M.; Černý, J. MHC II–EGFP Knock-in Mouse Model. Current Protocols 2023, 3 (11), e925. https://doi.org/https://doi.org/10.1002/cpz1.925.
1.
Nemcova, Y.; Faturova, J.; Škaloud, P. Comparing Continental and Local Distribution Patterns of Protists: A Case Study of Silica-Scaled Chrysophytes. Fottea 2023, 23 (2), 177–189. https://doi.org/10.5507/fot.2022.022.
1.
Pusztai, M.; Jadrná, I.; Škaloud, P. Elucidating the Phylogeny and Taxonomic Position of the Genus Spiniferomonas Takahashi (Chrysophyceae). Fottea 2023, 23 (2), 217–222. https://doi.org/10.5507/fot.2023.006.
1.
Knotek, P.; Škaloud, P. The Effect of Patterned Structures on the Mechanical Resistance of Microscopic Silica Scales. Fottea 2023, 23 (2), 190–200. https://doi.org/10.5507/fot.2023.007.
1.
Pomahac, O.; Mendez-Sanchez, D.; Polakova, K.; Mueller, M.; Solito, M.-M.; Bourland, W. A.; Cepicka, I. Rediscovery of Remarkably Rare Anaerobic Tentaculiferous Ciliate Genera Legendrea and Dactylochlamys (Ciliophora: Litostomatea). Biology-Basel 2023, 12 (5), 707. https://doi.org/10.3390/biology12050707.
1.
Dostál, V.; Humhalová, T.; Beránková, P.; Pácalt, O.; Libusová, L. SWIP Mediates Retromer-Independent Membrane Recruitment of the WASH Complex. Traffic 2023, 24 (5), 216–230. https://doi.org/https://doi.org/10.1111/tra.12884.
1.
Aumont, C.; Beránková, T.; McMahon, D. P.; Radek, R.; Akama, P. D.; Sillam-Dussès, D.; Šobotník, J. The Ultrastructure of the Rostral Gland in Soldiers of Verrucositermes Tuberosus (Blattodea: Termitidae: Nasutitermitinae). Arthropod Structure & Development 2023, 73, 101238. https://doi.org/10.1016/j.asd.2023.101238.
1.
Růžička, J.; Jakubec, P.; Mahlerová, K.; Šípková, H.; Nishikawa, M. Integrative Taxonomy and Species Distribution Models of the Genus Diamesus Hope, 1840 (Coleoptera: Staphylinidae: Silphinae). Sci Rep 2023, 13 (1), 3192. https://doi.org/10.1038/s41598-023-30019-x.
1.
Shewale, M. K.; Nebesářová, J.; Grosse-Wilde, E.; Kalinová, B. Microscopic Morphology and Distribution of the Antennal Sensilla in the Double-Spined Bark Beetle, Ips Duplicatus (Coleoptera: Curculionidae). Microscopy Research and Technique 2023, 86 (12), 1610–1625. https://doi.org/10.1002/jemt.24397.
1.
Méndez-Sánchez, D.; Pomahač, O.; Rotterová, J.; Bourland, W. A.; Čepička, I. Morphology and Phylogenetic Position of Three Anaerobic Ciliates from the Classes Odontostomatea and Muranotrichea (Ciliophora). Journal of Eukaryotic Microbiology 2023, 70 (3), e12965. https://doi.org/10.1111/jeu.12965.
1.
Stenc, J.; Janosík, L.; Matousková, E.; Hadrava, J.; Mikát, M.; Janovsky, Z. Pollinator Visitation Closely Tracks Diurnal Patterns in Pollen Release. AMERICAN JOURNAL OF BOTANY 2023, 110 (6). https://doi.org/10.1002/ajb2.16179.
1.
Poláková, K.; Bourland, W. A.; Cepicka, I. Anaerocyclidiidae Fam. Nov. (Oligohymenophorea, Scuticociliatia): A Newly Recognized Major Lineage of Anaerobic Ciliates Hosting Prokaryotic Symbionts. EUROPEAN JOURNAL OF PROTISTOLOGY 2023, 90. https://doi.org/10.1016/j.ejop.2023.126009.
1.
Budil, J.; Stenclová, P.; Kromka, A.; Lisková, P. Development of the Pseudomonas Syringae Pv. Morsprunorum Biofilm Monitored in Real Time Using Attenuated Total Reflection Fourier Transform Infrared Measurements in a Flow Cell Chamber. APPLIED SPECTROSCOPY 2023, 77 (5), 500–512. https://doi.org/10.1177/00037028231165057.
1.
Huber, B. A.; Meng, G. L.; Král, J.; Herrera, I. M. A.; Izquierdo, M. A.; Carvalho, L. S. High and Dry: Integrative Taxonomy of the Andean Spider Genus Nerudia (Araneae: Pholcidae). ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY 2023, 198 (2), 534–591. https://doi.org/10.1093/zoolinnean/zlac100.
1.
Majer, J.; Kindermann, M.; Pinkas, D.; Chvatil, D.; Cigler, P.; Libusova, L. Cellular Uptake and Fate of Cationic Polymer-Coated Nanodiamonds Delivering siRNA: A Mechanistic Study. NANOSCALE 2023. https://doi.org/10.1039/d3nr05738k.
1.
Huber, B. A.; Meng, G. L.; Kral, J.; Herrera, I. M. A.; Izquierdo, M. A. Revision of the South American Ninetinae Genus Guaranita (Araneae, Pholcidae). EUROPEAN JOURNAL OF TAXONOMY 2023, 900, 32–80. https://doi.org/10.5852/ejt.2023.900.2301.
1.
Mazancová, E.; Zadrobilková, E.; Yubuki, N.; Cepicka, I. Phylogenetic and Morphological Diversity of Free-Living Diplomonads. EUROPEAN JOURNAL OF PROTISTOLOGY 2023, 91. https://doi.org/10.1016/j.ejop.2023.126024.
1.
Hoboth, P.; Sztacho, M.; Quaas, A.; Akgül, B.; Hozák, P. Quantitative Super-Resolution Microscopy Reveals the Differences in the Nanoscale Distribution of Nuclear Phosphatidylinositol 4,5-Bisphosphate in Human Healthy Skin and Skin Warts. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 2023, 11. https://doi.org/10.3389/fcell.2023.1217637.
1.
Marvanova, A.; Kasik, P.; Elsnicova, B.; Tibenska, V.; Galatik, F.; Hornikova, D.; Zvolska, V.; Vebr, P.; Vodicka, P.; Hejnova, L.; Matous, P.; Bacova, B. S.; Sykora, M.; Novotny, J.; Neuzil, J.; Kolar, F.; Novakova, O.; Zurmanova, J. M. Continuous Short-Term Acclimation to Moderate Cold Elicits Cardioprotection in Rats, and Alters β-Adrenergic Signaling and Immune Status. Scientific Reports 2023, 13 (1), 18287. https://doi.org/10.1038/s41598-023-44205-4.
1.
Martinek, J.; Cifrová, P.; Vosolsobě, S.; García-González, J.; Malínská, K.; Mauerová, Z.; Jelínková, B.; Krtková, J.; Sikorová, L.; Leaves, I.; Sparkes, I.; Schwarzerová, K. ARP2/3 Complex Associates with Peroxisomes to Participate in Pexophagy in Plants. Nature Plants 2023, 9 (11), 1874–1889. https://doi.org/10.1038/s41477-023-01542-6.
2022
5891878
6T3MEUGG
1
https://raw.githubusercontent.com/Schebique/vmcf-konfmi/refs/heads/main/vmcf-web-style.csl
50
date
desc
4983
https://web.natur.cuni.cz/sekce-bi/VMCF/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22J3BXL7UZ%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Batelka%20et%20al.%22%2C%22parsedDate%22%3A%222022-12%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BBatelka%2C%20J.%3B%20Prokop%2C%20J.%3B%20Beutel%2C%20R.%20G.%20%26lt%3Bb%26gt%3BSystematic%20Position%20of%20Ptilophorus%20Dufourii%20Inferred%20from%20Its%20Primary%20Larva%2C%20with%20Notes%20on%20Ptilophorinae%20%28Coleoptera%3A%20Ripiphoridae%29%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BAnn.%20Zool.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2022%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B72%26lt%3B%5C%2Fi%26gt%3B%20%284%29%2C%20805%26%23x2013%3B826.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3161%5C%2F00034541ANZ2022.72.4.004%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3161%5C%2F00034541ANZ2022.72.4.004%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Systematic%20Position%20of%20Ptilophorus%20Dufourii%20Inferred%20from%20Its%20Primary%20Larva%2C%20with%20Notes%20on%20Ptilophorinae%20%28coleoptera%3A%20Ripiphoridae%29%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jan%22%2C%22lastName%22%3A%22Batelka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jakub%22%2C%22lastName%22%3A%22Prokop%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rolf%20G.%22%2C%22lastName%22%3A%22Beutel%22%7D%5D%2C%22abstractNote%22%3A%22Since%20the%20description%20of%20Ptilophorus%20dufourii%20%28Latreille%2C%201818%29%2C%20the%20unknown%20immature%20stages%20and%20biology%20of%20Ptilophorinae%20is%20one%20of%20the%20longest%20persisting%20gap%20in%20the%20research%20of%20the%20wedge-shaped%20beetles%20%28Ripiphoridae%29.%20Here%20we%20describe%20the%20primary%20larva%20of%20P.%20dufourii%20based%20on%20SEM%20and%20CLSM%20images.%20Its%20modified%20mouthparts%20unsuitable%20to%20grasp%20prey%20rule%20out%20predaceous%20habits%20and%20also%20processing%20any%20solid%20substrates%20%28e.g.%2C%20wood%20or%20other%20fresh%20plant%20tissues%29.%20In%20contrast%2C%20the%20weakly%20sclerotized%20and%20very%20small%20body%2C%20the%20enlargement%20of%20the%20sensorial%20appendage%20of%20the%20antennae%2C%20and%20simplified%20mouthparts%20strongly%20suggest%20parasitism%20and%20consumption%20of%20liquid%20food.%20We%20provide%20a%20comparison%20with%20the%20only%20known%20larva%20of%20Pelecotominae%2C%20another%20possible%20basal%20lineage%20of%20Ripiphoridae%2C%20and%20discuss%20potential%20synapomorphies%20and%20differences%20between%20larvae%20of%20both%20subfamilies.%20A%20sistergroup%20relationship%20between%20Ptilophorinae%20and%20Pelecotominae%20is%20suggested%20by%20the%20presence%20of%20only%20two%20stemmata%2C%20spatulate%20femoral%20setae%2C%20a%20scale-like%20lateral%20cranial%20surface%2C%20and%20an%20enlarged%20antennal%20sensorial%20appendage%2C%20and%20a%20clade%20Ripidiini%20%2B%20Ripiphorinae%20by%20a%20characteristic%20boat-shape%20of%20the%20primary%20larvae.%20The%20host%20of%20P.%20dufourii%20remains%20unknown.%20We%20reviewed%20published%20host%20records%2C%20but%20none%20of%20them%20is%20based%20on%20traceable%20evidence%20and%20none%20of%20them%20appears%20credible.%20Observations%20of%20adult%20behaviour%20of%20P.%20dufourii%20are%20documented.%20Taxonomy%20and%20systematics%20of%20Ptilophorus%20and%20Ptilophorinae%20are%20discussed.%20The%20Cretaceous%20Spinotoma%20ruicheni%20Hsiao%20et%20Huang%2C%202017%20is%20transferred%20from%20Pelecotominae%20to%20Ptilophorinae%20in%20accordance%20with%20diagnostic%20characters%20given%20in%20the%20original%20description%20and%20current%20definitions%20of%20both%20subfamilies.%22%2C%22date%22%3A%22DEC%202022%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3161%5C%2F00034541ANZ2022.72.4.004%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220003-4541%2C%201734-1833%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%226T3MEUGG%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A23%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22RYEPAWEY%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Pil%5Cu00e1tov%5Cu00e1%20et%20al.%22%2C%22parsedDate%22%3A%222022-09-01%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BPil%26%23xE1%3Btov%26%23xE1%3B%2C%20J.%3B%20P%26%23xE1%3Bnek%2C%20T.%3B%20Oborn%26%23xED%3Bk%2C%20M.%3B%20%26%23x10C%3Bepi%26%23x10D%3Bka%2C%20I.%3B%20Mojze%26%23x161%3B%2C%20P.%20%26lt%3Bb%26gt%3BRevisiting%20Biocrystallization%3A%20Purine%20Crystalline%20Inclusions%20Are%20Widespread%20in%20Eukaryotes%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BISME%20J%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2022%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B16%26lt%3B%5C%2Fi%26gt%3B%20%289%29%2C%202290%26%23x2013%3B2294.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-ItemURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41396-022-01264-1%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41396-022-01264-1%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Revisiting%20biocrystallization%3A%20purine%20crystalline%20inclusions%20are%20widespread%20in%20eukaryotes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jana%22%2C%22lastName%22%3A%22Pil%5Cu00e1tov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tom%5Cu00e1%5Cu0161%22%2C%22lastName%22%3A%22P%5Cu00e1nek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Miroslav%22%2C%22lastName%22%3A%22Oborn%5Cu00edk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ivan%22%2C%22lastName%22%3A%22%5Cu010cepi%5Cu010dka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Mojze%5Cu0161%22%7D%5D%2C%22abstractNote%22%3A%22Despite%20the%20widespread%20occurrence%20of%20intracellular%20crystalline%20inclusions%20in%20unicellular%20eukaryotes%2C%20scant%20attention%20has%20been%20paid%20to%20their%20composition%2C%20functions%2C%20and%20evolutionary%20origins.%20Using%20Raman%20microscopy%2C%20we%20examined%20%26gt%3B200%20species%20from%20all%20major%20eukaryotic%20supergroups.%20We%20detected%20cellular%20crystalline%20inclusions%20in%2077%25%20species%20out%20of%20which%2080%25%20is%20composed%20of%20purines%2C%20such%20as%20anhydrous%20guanine%20%2862%25%29%2C%20guanine%20monohydrate%20%282%25%29%2C%20uric%20acid%20%2812%25%29%20and%20xanthine%20%284%25%29.%20Our%20findings%20shifts%20the%20paradigm%20assuming%20predominance%20of%20calcite%20and%20oxalates.%20Purine%20crystals%20emerge%20in%20microorganisms%20in%20all%20habitats%2C%20e.g.%2C%20in%20freshwater%20algae%2C%20endosymbionts%20of%20reef-building%20corals%2C%20deadly%20parasites%2C%20anaerobes%20in%20termite%20guts%2C%20or%20slime%20molds.%20Hence%2C%20purine%20biocrystallization%20is%20a%20general%20and%20ancestral%20eukaryotic%20process%20likely%20present%20in%20the%20last%20eukaryotic%20common%20ancestor%20%28LECA%29%20and%20here%20we%20propose%20two%20proteins%20omnipresent%20in%20eukaryotes%20that%20are%20likely%20in%20charge%20of%20their%20metabolism%3A%20hypoxanthine-guanine%20phosphoribosyl%20transferase%20and%20equilibrative%20nucleoside%20transporter.%20Purine%20crystalline%20inclusions%20are%20multifunctional%20structures%20representing%20high-capacity%20and%20rapid-turnover%20reserves%20of%20nitrogen%20and%20optically%20active%20elements%2C%20e.g.%2C%20used%20in%20light%20sensing.%20Thus%2C%20we%20anticipate%20our%20work%20to%20be%20a%20starting%20point%20for%20further%20studies%20spanning%20from%20cell%20biology%20to%20global%20ecology%2C%20with%20potential%20applications%20in%20biotechnologies%2C%20bio-optics%2C%20or%20in%20human%20medicine.%22%2C%22date%22%3A%222022-09-01%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41396-022-01264-1%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41396-022-01264-1%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221751-7362%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%226T3MEUGG%22%5D%2C%22dateModified%22%3A%222025-09-06T09%3A13%3A28Z%22%7D%7D%2C%7B%22key%22%3A%227MWQXL6C%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Mendez-Sanchez%20et%20al.%22%2C%22parsedDate%22%3A%222022-08%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BMendez-Sanchez%2C%20D.%3B%20Pomahac%2C%20O.%3B%20Rotterova%2C%20J.%3B%20Bourland%2C%20W.%3B%20Cepicka%2C%20I.%20%26lt%3Bb%26gt%3BDiversity%20and%20Phylogenetic%20Position%20of%20Bothrostoma%20Stokes%2C%201887%20%28Ciliophora%3A%20Metopida%29%2C%20with%20Description%20of%20Four%20New%20Species%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BProtist%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2022%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B173%26lt%3B%5C%2Fi%26gt%3B%20%284%29%2C%20125887.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.protis.2022.125887%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.protis.2022.125887%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Diversity%20and%20Phylogenetic%20Position%20of%20Bothrostoma%20Stokes%2C%201887%20%28Ciliophora%3A%20Metopida%29%2C%20with%20Description%20of%20Four%20New%20Species%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%22%2C%22lastName%22%3A%22Mendez-Sanchez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ondrej%22%2C%22lastName%22%3A%22Pomahac%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Johana%22%2C%22lastName%22%3A%22Rotterova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22William%22%2C%22lastName%22%3A%22Bourland%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ivan%22%2C%22lastName%22%3A%22Cepicka%22%7D%5D%2C%22abstractNote%22%3A%22Bothrostoma%20is%20a%20genus%20of%20anaerobic%20ciliates%20in%20family%20Metopidae%20comprising%20four%20species%2C%20all%20described%20based%20solely%20on%20the%20morphology%20of%20living%20and%20fixed%20cells.%20Unlike%20other%20metopids%2C%20cells%20of%20Bothrostoma%20are%20not%20twisted%20anteriorly%2C%20have%20a%20flattened%20preoral%20dome%2C%20a%20very%20prominent%20sail-like%20paroral%20membrane%2C%20and%20an%20adoral%20zone%20of%20distinctive%2C%20very%20narrow%2C%20curved%20membranelles%20confined%20to%20a%20wide%2C%20non-spiraling%20peristome%20on%20the%20ventral%20side.%20We%20examined%2020%20populations%20of%20Bothrostoma%20from%20hypoxic%20freshwater%20sediments.%20We%20provide%20morphological%20characterization%20and%2018S%20rRNA%20gene%20sequences%20of%20four%20new%20species%2C%20namely%20B.%20bimicronucleatum%20sp.%20nov.%2C%20B.%20boreale%20sp.%20nov.%2C%20B.%20kovalyovi%20sp.%20nov.%2C%20and%20B.%20robustum%20sp.%20nov.%2C%20as%20well%20as%20B.%20undulans%20%28type%20species%29%2C%20B.%20nasutum%2C%20and%20B.%20ovale%20comb.%20nov.%20%28original%20combination%20Metopus%20undulans%20var.%20ovalis%20Kahl%2C%201932%29.%20Except%20for%20B.%20nasutum%2C%20Bothrostoma%20species%20show%20low%20genetic%20variability%20among%20geographically%20distant%20populations.%20Intraspecific%20phenotypic%20variability%20might%20be%20driven%20by%20environmental%20conditions.%20In%20phylogenetic%20analyses%2C%20Bothrostoma%20is%20not%20closely%20related%20to%20Metopus%20sensu%20stricto%20and%20forms%20a%20moderately%20supported%20clade%20with%20Planometopus%2C%20here%20referred%20to%20as%20BoPl%20clade.%20The%20anterior%20axial%20torsion%20of%20the%20body%2C%20typical%20of%20other%20Metopidae%2C%20appears%20to%20have%20been%20lost%20in%20the%20last%20common%20ancestor%20of%20the%20BoPl%20clade.%20%28c%29%202022%20Elsevier%20GmbH.%20All%20rights%20reserved.%22%2C%22date%22%3A%22AUG%202022%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.protis.2022.125887%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221434-4610%2C%201618-0941%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%226T3MEUGG%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A23%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22AY7WU2JQ%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Glassnerova%20et%20al.%22%2C%22parsedDate%22%3A%222022-06%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BGlassnerova%2C%20K.%3B%20Sklenar%2C%20F.%3B%20Jurjevic%2C%20Z.%3B%20Houbraken%2C%20J.%3B%20Yaguchi%2C%20T.%3B%20Visagie%2C%20C.%20M.%3B%20Gene%2C%20J.%3B%20Siqueira%2C%20J.%20P.%20Z.%3B%20Kubatova%2C%20A.%3B%20Kolarik%2C%20M.%3B%20Hubka%2C%20V.%20%26lt%3Bb%26gt%3BA%20Monograph%20of%20Aspergillus%20Section%20Candidi%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BStud.%20Mycol.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2022%26lt%3B%5C%2Fb%26gt%3B%2C%20No.%20102%2C%201%26%23x2013%3B51.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3114%5C%2Fsim.2022.102.01%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3114%5C%2Fsim.2022.102.01%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20monograph%20of%20Aspergillus%20section%20Candidi%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Glassnerova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22Sklenar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Z.%22%2C%22lastName%22%3A%22Jurjevic%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Houbraken%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%22%2C%22lastName%22%3A%22Yaguchi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20M.%22%2C%22lastName%22%3A%22Visagie%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Gene%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20P.%20Z.%22%2C%22lastName%22%3A%22Siqueira%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Kubatova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Kolarik%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Hubka%22%7D%5D%2C%22abstractNote%22%3A%22Aspergillus%20section%20Candidi%20encompasses%20white-%20or%20yellow-sporulating%20species%20mostly%20isolated%20from%20indoor%20and%20cave%20environments%2C%20food%2C%20feed%2C%20clinical%20material%2C%20soil%20and%20dung.%20Their%20identification%20is%20non-trivial%20due%20to%20largely%20uniform%20morphology.%20This%20study%20aims%20to%20re-evaluate%20the%20species%20boundaries%20in%20the%20section%20Candidi%20and%20present%20an%20overview%20of%20all%20existing%20species%20along%20with%20information%20on%20their%20ecology.%20For%20the%20analyses%2C%20we%20assembled%20a%20set%20of%20113%20strains%20with%20diverse%20origin.%20For%20the%20molecular%20analyses%2C%20we%20used%20DNA%20sequences%20of%20three%20house-keeping%20genes%20%28benA%2C%20CaM%20and%20RPB2%29%20and%20employed%20species%20delimitation%20methods%20based%20on%20a%20multispecies%20coalescent%20model.%20Classical%20phylogenetic%20methods%20and%20genealogical%20concordance%20phylogenetic%20species%20recognition%20%28GCPSR%29%20approaches%20were%20used%20for%20comparison.%20Phenotypic%20studies%20involved%20comparisons%20of%20macromorphology%20on%20four%20cultivation%20media%2C%20seven%20micromorphological%20characters%20and%20growth%20at%20temperatures%20ranging%20from%2010%20to%2045%20degrees%20C.%20Based%20on%20the%20integrative%20approach%20comprising%20four%20criteria%20%28phylogenetic%20and%20phenotypic%29%2C%20all%20currently%20accepted%20species%20gained%20support%2C%20while%20two%20new%20species%20are%20proposed%20%28A.%20magnus%20and%20A.%20tenebricus%29.%20In%20addition%2C%20we%20proposed%20the%20new%20name%20A.%20neotritici%20to%20replace%20an%20invalidly%20described%20A.%20tritici.%20The%20revised%20section%20Candidi%20now%20encompasses%20nine%20species%2C%20some%20of%20which%20manifest%20a%20high%20level%20of%20intraspecific%20genetic%20and%5C%2For%20phenotypic%20variability%20%28e.g.%2C%20A.%20subalbidus%20and%20A.%20campestris%29%20while%20others%20are%20more%20uniform%20%28e.g.%2C%20A.%20candidus%20or%20A.%20pragensis%29.%20The%20growth%20rates%20on%20different%20media%20and%20at%20different%20temperatures%2C%20colony%20colours%2C%20production%20of%20soluble%20pigments%2C%20stipe%20dimensions%20and%20vesicle%20diameters%20contributed%20the%20most%20to%20the%20phenotypic%20species%20differentiation.%22%2C%22date%22%3A%22JUN%202022%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3114%5C%2Fsim.2022.102.01%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220166-0616%2C%201872-9797%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%226T3MEUGG%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A23%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22J4T6DBL6%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Berankova%20et%20al.%22%2C%22parsedDate%22%3A%222022-03%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BBerankova%2C%20T.%3B%20Bucek%2C%20A.%3B%20Bourguignon%2C%20T.%3B%20Arias%2C%20J.%20R.%3B%20Akama%2C%20P.%20D.%3B%20Sillam-Dusses%2C%20D.%3B%20Sobotnik%2C%20J.%20%26lt%3Bb%26gt%3BThe%20Ultrastructure%20of%20the%20Intramandibular%20Gland%20in%20Soldiers%20of%20the%20Termite%20Machadotermes%20Rigidus%20%28Blattodea%3A%20Termitidae%3A%20Apicotermitinae%29%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BArthropod%20Struct.%20Dev.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2022%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B67%26lt%3B%5C%2Fi%26gt%3B%2C%20101136.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.asd.2021.101136%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.asd.2021.101136%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20ultrastructure%20of%20the%20intramandibular%20gland%20in%20soldiers%20of%20the%20termite%20Machadotermes%20rigidus%20%28Blattodea%3A%20Termitidae%3A%20Apicotermitinae%29%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tereza%22%2C%22lastName%22%3A%22Berankova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ales%22%2C%22lastName%22%3A%22Bucek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%22%2C%22lastName%22%3A%22Bourguignon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Johanna%20Romero%22%2C%22lastName%22%3A%22Arias%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pierre%20D.%22%2C%22lastName%22%3A%22Akama%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Sillam-Dusses%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jan%22%2C%22lastName%22%3A%22Sobotnik%22%7D%5D%2C%22abstractNote%22%3A%22Machadotermes%20is%20one%20of%20the%20basal%20Apicotermitinae%20genera%2C%20living%20in%20tropical%20West%20Africa.%20Old%20observations%20suggested%20the%20presence%20of%20a%20new%20gland%2C%20the%20intramandibular%20gland%2C%20in%20Machadotermes%20soldiers.%20Here%2C%20by%20combining%20micro-computed%20tomography%2C%20optical%20and%20electron%20microscopy%2C%20we%20showed%20that%20the%20gland%20exists%20in%20Machadotermes%20soldiers%20only%20as%20an%20active%20exocrine%20organ%2C%20consisting%20of%20numerous%20class%20III%20cells%20%28bicellular%20units%20made%20of%20secretory%20and%20canal%20cells%29%2C%20within%20which%20the%20secretion%20is%20produced%20in%20rough%20endoplasmic%20reticulum%2C%20and%20modified%20and%20stored%20in%20Golgi%20apparatus.%20The%20final%20secretion%20is%20released%20out%20from%20the%20body%20through%20epicuticular%20canals%20running%20through%20the%20mandible%20cuticle%20to%20the%20exterior.%20We%20also%20studied%20three%20other%20Apicotermitinae%2C%20Indotermes%2C%20Duplidentitermes%2C%20and%20Jugositermes%2C%20in%20which%20this%20gland%20is%20absent.%20We%20speculate%20that%20the%20secretion%20of%20this%20gland%20may%20be%20used%20as%20a%20general%20protectant%20or%20antimicrobial%20agent.%20In%20addition%2C%20we%20observed%20that%20the%20frontal%20gland%2C%20a%20specific%20defensive%20organ%20in%20termites%2C%20is%20absent%20in%20Machadotermes%20soldiers%20while%20it%20is%20tiny%20in%20Indotermes%20soldiers%20and%20small%20in%20Duplidentitermes%20and%20Jugositermes%20soldiers.%20At%20last%2C%20we%20could%20also%20observe%20in%20all%20these%20species%20the%20labral%2C%20mandibular%20and%20labial%20glands%2C%20other%20exocrine%20glands%20present%20in%20all%20termite%20species%20studied%20so%20far.%28c%29%202021%20Published%20by%20Elsevier%20Ltd.%22%2C%22date%22%3A%22MAR%202022%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.asd.2021.101136%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221467-8039%2C%201873-5495%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%226T3MEUGG%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A23%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22B883UGJW%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Nemcova%20et%20al.%22%2C%22parsedDate%22%3A%222022-02%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BNemcova%2C%20Y.%3B%20Faturova%2C%20J.%3B%20Kreidlova%2C%20J.%20%26lt%3Bb%26gt%3BThe%20New%20Species%20of%20Mallomonas%20%28Stramenopiles%2C%20Chrysophyceae%2C%20Synurales%29%3A%20Mallomonas%20Decora%20Sp.%20Nov.%26lt%3B%5C%2Fb%26gt%3B%20%26lt%3Bi%26gt%3BNova%20Hedwigia%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2022%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B114%26lt%3B%5C%2Fi%26gt%3B%20%281%26%23x2013%3B2%29%2C%201%26%23x2013%3B8.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1127%5C%2Fnova_hedwigia%5C%2F2022%5C%2F0677%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1127%5C%2Fnova_hedwigia%5C%2F2022%5C%2F0677%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20new%20species%20of%20Mallomonas%20%28Stramenopiles%2C%20Chrysophyceae%2C%20Synurales%29%3A%20Mallomonas%20decora%20sp.%20nov.%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yvonne%22%2C%22lastName%22%3A%22Nemcova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jana%22%2C%22lastName%22%3A%22Faturova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jana%22%2C%22lastName%22%3A%22Kreidlova%22%7D%5D%2C%22abstractNote%22%3A%22Here%2C%20we%20describe%20the%20new%20species%20Mallomonas%20decora%20that%20was%20first%20detected%20in%20two%20small%20ponds%20in%20the%20Czech%20Republic.%20The%20description%20is%20based%20on%20silica-scale%20morphology%2C%20employing%20both%20transmission%20%28TEM%29%20and%20scanning%20electron%20microscopy%20%28SEM%29.%20Possessing%20only%20apical%20bristles%20attached%20to%20the%20most%20anterior%20ring%20od%20collar%20scales%2C%20Mallomonas%20decora%20belongs%20to%20the%20section%20Torquatae.%20The%20shield%20of%20the%20collar%20scale%20is%20covered%20with%20ribs%20that%20are%20transversely%20oriented%3B%20towards%20the%20central%20part%20of%20the%20shield%2C%20the%20ribs%20are%20branched%20and%5C%2For%20form%20a%20reticulate%20honeycomb%20structure.%20The%20anterior%20submarginal%20rib%20is%20flattened%2C%20supporting%20the%20anterior%20submarginal%20flange%2C%20that%20is%20covered%20with%20conspicuous%20ribs.%20The%20taxa%20that%20are%20the%20most%20similar%20to%20M.%20decora%20are%20M.%20dickii%20and%20M.%20ocalensis.%20Mallomonas%20dickii%20differs%20from%20M.%20decora%20by%20the%20presence%20of%20large%20pores%20located%20between%20pairs%20of%20ribs%20on%20the%20anterior%20flange.%20The%20ribs%20on%20the%20shield%20of%20M.%20dickii%20are%20also%20spaced%20more%20closely.%20Mallomonas%20ocalensis%20differs%20from%20M.%20decora%20by%20having%20the%20protruding%20anterior%20submarginal%20rib%2C%20ornamented%20with%20the%20small%20papillae%20and%20short%20struts%20connecting%20it%20to%20the%20base%20plate.%22%2C%22date%22%3A%22FEB%202022%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1127%5C%2Fnova_hedwigia%5C%2F2022%5C%2F0677%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220029-5035%2C%202363-7188%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%226T3MEUGG%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A23%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22JP6UWZJW%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Bellinvia%20et%20al.%22%2C%22parsedDate%22%3A%222022%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BBellinvia%2C%20E.%3B%20Garc%26%23xED%3Ba-Gonz%26%23xE1%3Blez%2C%20J.%3B%20Cifrov%26%23xE1%3B%2C%20P.%3B%20Martinek%2C%20J.%3B%20Sikorov%26%23xE1%3B%2C%20L.%3B%20Havelkov%26%23xE1%3B%2C%20L.%3B%20Schwarzerov%26%23xE1%3B%2C%20K.%20%26lt%3Bb%26gt%3BCRISPR-Cas9%20Arabidopsis%20Mutants%20of%20Genes%20for%20ARPC1%20and%20ARPC3%20Subunits%20of%20ARP2%5C%2F3%20Complex%20Reveal%20Differential%20Roles%20of%20Complex%20Subunits%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BSCIENTIFIC%20REPORTS%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2022%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B12%26lt%3B%5C%2Fi%26gt%3B%20%281%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-022-22982-8%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-022-22982-8%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22CRISPR-Cas9%20Arabidopsis%20mutants%20of%20genes%20for%20ARPC1%20and%20ARPC3%20subunits%20of%20ARP2%5C%2F3%20complex%20reveal%20differential%20roles%20of%20complex%20subunits%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Bellinvia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Garc%5Cu00eda-Gonz%5Cu00e1lez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Cifrov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Martinek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Sikorov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Havelkov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Schwarzerov%5Cu00e1%22%7D%5D%2C%22abstractNote%22%3A%22Protein%20complex%20Arp2%5C%2F3%20has%20a%20conserved%20role%20in%20the%20nucleation%20of%20branched%20actin%20filaments.%20It%20is%20constituted%20of%20seven%20subunits%2C%20including%20actin-like%20subunits%20ARP2%20and%20ARP3%20plus%20five%20other%20subunits%20called%20Arp2%5C%2F3%20Complex%20Component%201%20to%205%2C%20which%20are%20not%20related%20to%20actin.%20Knock-out%20plant%20mutants%20lacking%20individual%20plant%20ARP2%5C%2F3%20subunits%20have%20a%20typical%20phenotype%20of%20distorted%20trichomes%2C%20altered%20pavement%20cells%20shape%20and%20defects%20in%20cell%20adhesion.%20While%20knock-out%20mutant%20Arabidopsis%20plants%20for%20most%20ARP2%5C%2F3%20subunits%20have%20been%20characterized%20before%2C%20Arabidopsis%20plant%20mutants%20missing%20ARPC1%20and%20ARPC3%20subunits%20have%20not%20yet%20been%20described.%20Using%20CRISPR%5C%2FCas9%2C%20we%20generated%20knock-out%20mutants%20lacking%20ARPC1%20and%20ARPC3%20subunits.%20We%20confirmed%20that%20the%20loss%20of%20ARPC1%20subunits%20results%20in%20the%20typical%20ARP2%5C%2F3%20mutant%20phenotype.%20However%2C%20the%20mutants%20lacking%20ARPC3%20subunits%20resulted%20in%20plants%20with%20surprisingly%20different%20phenotypes.%20Our%20results%20suggest%20that%20plant%20ARP2%5C%2F3%20complex%20function%20in%20trichome%20shaping%20does%20not%20require%20ARPC3%20subunit%2C%20while%20the%20fully%20assembled%20complex%20is%20necessary%20for%20the%20establishment%20of%20correct%20cell%20adhesion%20in%20the%20epidermis.%22%2C%22date%22%3A%222022%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-022-22982-8%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%222045-2322%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%226T3MEUGG%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%22UVGKNREK%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Paces%20et%20al.%22%2C%22parsedDate%22%3A%222022%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BPaces%2C%20J.%3B%20Kn%26%23xED%3Bzkov%26%23xE1%3B%2C%20K.%3B%20Tuskov%26%23xE1%3B%2C%20L.%3B%20Grob%26%23xE1%3Brov%26%23xE1%3B%2C%20V.%3B%20Zadrazil%2C%20Z.%3B%20Boes%2C%20M.%3B%20Cerny%2C%20J.%20%26lt%3Bb%26gt%3BMHC%20II-EGFP%20Knock-in%20Mouse%20Model%20Is%20a%20Suitable%20Tool%20for%20Systems%20and%20Quantitative%20Immunology%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BIMMUNOLOGY%20LETTERS%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2022%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B251%26%23x2013%3B252%26lt%3B%5C%2Fi%26gt%3B%2C%2075%26%23x2013%3B85.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.imlet.2022.10.007%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.imlet.2022.10.007%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22MHC%20II-EGFP%20knock-in%20mouse%20model%20is%20a%20suitable%20tool%20for%20systems%20and%20quantitative%20immunology%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Paces%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Kn%5Cu00edzkov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Tuskov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Grob%5Cu00e1rov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Z.%22%2C%22lastName%22%3A%22Zadrazil%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Boes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Cerny%22%7D%5D%2C%22abstractNote%22%3A%22Immunology%20is%20a%20rapidly%20evolving%20field%20of%20research%20with%20sophisticated%20models%20and%20methods.%20However%2C%20detailed%20data%20on%20total%20immune%20cell%20counts%20and%20population%20distributions%20remain%20surprisingly%20scarce.%20Nevertheless%2C%20recently%20established%20quantitative%20approaches%20could%20help%20us%20understand%20the%20overall%20complexity%20of%20the%20immune%20system.%20Here%2C%20we%20studied%20a%20major%20histocompatibility%20complexclass%20II%20-%20enhanced%20green%20fluorescent%20protein%20knock-in%20mouse%20model%20to%20precisely%20identify%20and%20manipulate%20lymphoid%20structures.%20By%20combining%20flow%20cytometry%20with%20light%20sheet%20microscopy%2C%20we%20quantified%20MHC%20II%2B%20populations%20of%20the%20small%20intestine%20and%20associated%20individual%20mesenteric%20lymph%20nodes%2C%20with%2036.7%20x%20106%20cells%20in%20lamina%20propria%2C%203.0%20x%20105%20cells%20in%20scattered%20lymphoid%20tissue%20and%201.1%20x%20106%20cells%20in%20Peyer%26%23039%3Bs%20patches.%20In%20addition%20to%20these%20whole-organ%20cell%20counts%2C%20we%20assessed%20approximately%201%20x%20106%20total%20villi%20in%20the%20small%20intestine%20and%20450%20scattered%20lymphoid%20tissue%20follicles.%20By%20direct%20noninvasive%20microscopic%20observation%20of%20a%20naturally%20fully%20translucent%20mouse%20organ%2C%20the%20cornea%2C%20we%20quantified%2012%20%2B%5C%2F-%204%20and%2035%20%2B%5C%2F-%207%20cells%5C%2Fmm2%20Langerhans-and%20macrophage-like%20populations%2C%20respectively.%20Ultimately%2C%20our%20findings%20show%20that%20flow%20cytometry%20with%20quantitative%20imaging%20data%20analysis%20enables%20us%20to%20avoid%20methodological%20discrepancies%20while%20gaining%20new%20insights%20into%20the%20relevance%20of%20organ-specific%20quantitative%20approaches%20for%20immunology.%22%2C%22date%22%3A%222022%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.imlet.2022.10.007%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220165-2478%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%226T3MEUGG%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%22GLFWSSJF%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Just%20et%20al.%22%2C%22parsedDate%22%3A%222022%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BJust%2C%20P.%3B%20St%26%23xE1%3Bhlavsky%2C%20F.%3B%20Kovar%26%23xED%3Bk%2C%20F.%3B%20Stundlov%26%23xE1%3B%2C%20J.%20%26lt%3Bb%26gt%3BTracking%20the%20Trends%20of%20Karyotype%20Differentiation%20in%20the%20Phylogenetic%20Context%20of%20Gint%2C%20a%20Scorpion%20Genus%20Endemic%20to%20the%20Horn%20of%20Africa%20%28Scorpiones%3A%20Buthidae%29%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BZOOLOGICAL%20JOURNAL%20OF%20THE%20LINNEAN%20SOCIETY%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2022%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B196%26lt%3B%5C%2Fi%26gt%3B%20%282%29%2C%20885%26%23x2013%3B901.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fzoolinnean%5C%2Fzlac049%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fzoolinnean%5C%2Fzlac049%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Tracking%20the%20trends%20of%20karyotype%20differentiation%20in%20the%20phylogenetic%20context%20of%20Gint%2C%20a%20scorpion%20genus%20endemic%20to%20the%20Horn%20of%20Africa%20%28Scorpiones%3A%20Buthidae%29%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Just%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22St%5Cu00e1hlavsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22Kovar%5Cu00edk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Stundlov%5Cu00e1%22%7D%5D%2C%22abstractNote%22%3A%22To%20determine%20the%20mechanisms%20of%20karyotype%20differentiation%20in%20scorpions%20of%20the%20genus%20Gint%2C%20we%20employed%20an%20integrative%20approach%2C%20combining%20cytogenetic%20data%20and%20sequence-based%20phylogeny.%20We%20cytogenetically%20examined%20six%20species%20with%20emphasis%20on%20multivalent%20meiotic%20configurations%2C%2018S%20rDNA%20and%20%28TTAGG%29n%20distribution%20and%20compared%20chromosomal%20data%20with%20genetic%20divergence%20based%20on%20analysis%20of%2016S%20rRNA%20and%20COI%20gene%20markers.%20Our%20results%20show%20that%20Gint%20species%20exhibit%20substantial%20karyotype%20diversity%20%282n%20%3D%2018-45%29%20and%20a%20high%20incidence%20of%20chromosome%20heterozygosity.%20Meiotic%20chromosome%20chains%20formed%20by%20up%20to%20six%20elements%20were%20found%20in%2085%25%20of%20analysed%20individuals%2C%20causing%20intraspecific%20chromosome%20variation%20in%20three%20species.%20Fluorescence%20in%20situ%20hybridization%20revealed%20that%20the%2018S%20rDNA%20distribution%20pattern%20differed%20in%20Gint%20species%2C%20including%20at%20the%20intrapopulation%20level%2C%20but%20the%20chromosomal%20localization%20of%20%28TTAGG%29n%20motif%20was%20stable%20across%20species.%20Conspicuous%20interspecific%20differences%20in%20chromosome%20counts%20broadly%20corresponded%20with%20genetic%20divergence%20among%20Gint%20species.%20Our%20findings%20indicate%20that%20Gint%20karyotypes%20have%20undergone%20dynamic%20reorganization%20through%20independent%20fusions%2C%20fissions%20and%20reciprocal%20translocations.%20Owing%20to%20present%20chromosomal%20polymorphism%2C%20such%20structural%20changes%20shaping%20the%20genome%20architecture%20appear%20to%20be%20still%20ongoing%20in%20the%20populations%20of%20some%20Gint%20species.%22%2C%22date%22%3A%222022%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fzoolinnean%5C%2Fzlac049%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220024-4082%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%226T3MEUGG%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%222XYUF4FF%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Kr%5Cu00e1l%20et%20al.%22%2C%22parsedDate%22%3A%222022%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BKr%26%23xE1%3Bl%2C%20J.%3B%20Herrera%2C%20I.%20M.%20A.%3B%20St%26%23xE1%3Bhlavsky%2C%20F.%3B%20Sad%26%23xED%3Blek%2C%20D.%3B%20Pavelka%2C%20J.%3B%20Chatzaki%2C%20M.%3B%20Huber%2C%20B.%20A.%20%26lt%3Bb%26gt%3BKaryotype%20Differentiation%20and%20Male%20Meiosis%20in%20European%20Clades%20of%20the%20Spider%20Genus%26lt%3Bi%26gt%3B%20Pholcus%26lt%3B%5C%2Fi%26gt%3B%20%28Araneae%2C%20Pholcidae%29%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BCOMPARATIVE%20CYTOGENETICS%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2022%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B16%26lt%3B%5C%2Fi%26gt%3B%20%284%29%2C%20185%26%23x2013%3B209.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3897%5C%2Fcompcytogen.v16.i4.85059%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3897%5C%2Fcompcytogen.v16.i4.85059%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Karyotype%20differentiation%20and%20male%20meiosis%20in%20European%20clades%20of%20the%20spider%20genus%3Ci%3E%20Pholcus%3C%5C%2Fi%3E%20%28Araneae%2C%20Pholcidae%29%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Kr%5Cu00e1l%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22I.%20M.%20A.%22%2C%22lastName%22%3A%22Herrera%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22St%5Cu00e1hlavsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Sad%5Cu00edlek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Pavelka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Chatzaki%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20A.%22%2C%22lastName%22%3A%22Huber%22%7D%5D%2C%22abstractNote%22%3A%22Haplogyne%20araneomorphs%20are%20a%20diverse%20spider%20clade.%20Their%20karyotypes%20are%20usually%20predominated%20by%20biarmed%20%28i.e.%2C%20metacentric%20and%20submetacentric%29%20chromosomes%20and%20have%20a%20specific%20sex%20chromosome%20system%2C%20X1X2Y.%20These%20features%20are%20probably%20ancestral%20for%20haplogynes.%20Nucleolus%20organizer%20regions%20%28NORs%29%20spread%20fre-quently%20from%20autosomes%20to%20sex%20chromosomes%20in%20these%20spiders.%20This%20study%20focuses%20on%20pholcids%20%28Pholcidae%29%2C%20a%20highly%20diverse%20haplogyne%20family.%20Despite%20considerable%20recent%20progress%20in%20pholcid%20cytogenetics%2C%20knowledge%20on%20many%20clades%20remains%20insufficient%20including%20the%20most%20species-rich%20pholcid%20genus%2C%20Pholcus%20Walckenaer%2C%201805.%20To%20characterize%20the%20karyotype%20differentiation%20of%20Pholcus%20in%20Europe%2C%20we%20compared%20karyotypes%2C%20sex%20chromosomes%2C%20NORs%2C%20and%20male%20meiosis%20of%20seven%20species%20%5BP.%20alticeps%20Spassky%2C%201932%3B%20P.%20creticus%20Senglet%2C%201971%3B%20P.%20dentatus%20Wunderlich%2C%201995%3B%20P.%20fuerteventurensis%20Wunderlich%2C%201992%3B%20P.%20phalangioides%20%28Fuesslin%2C%201775%29%3B%20P.%20opilionoides%20%28Schrank%2C%201781%29%3B%20P.%20silvai%20Wunderlich%2C%201995%5D%20representing%20the%20dominant%20species%20groups%20in%20this%20region.%20The%20species%20studied%20show%20several%20features%20ancestral%20for%20Pholcus%2C%20namely%20the%202n%20male%20%3D%2025%2C%20the%20X1X2Y%20system%2C%20and%20a%20karyotype%20predominated%20by%20biarmed%20chromosomes.%20Most%20taxa%20have%20a%20large%20acro-centric%20NOR-bearing%20pair%2C%20which%20evolved%20from%20a%20biarmed%20pair%20by%20a%20pericentric%20inversion.%20In%20some%20lineages%2C%20the%20acrocentric%20pair%20reverted%20to%20biarmed.%20Closely%20related%20species%20often%20differ%20in%20the%20morphology%20of%20some%20chromosome%20pairs%2C%20probably%20resulting%20from%20pericentric%20inversions%20and%5C%2For%20translocations.%20Such%20rearrange-ments%20have%20been%20implicated%20in%20the%20formation%20of%20reproductive%20barriers.%20While%20the%20X1%20and%20Y%20chromosomes%20retain%20their%20ancestral%20metacentric%20morphology%2C%20the%20X2%20chromosome%20shows%20a%20derived%20%28acrocentric%20or%20sub-telocentric%29%20morphology.%20Pairing%20of%20this%20element%20is%20usually%20modified%20during%20male%20meiosis.%20NOR%20patterns%20are%20very%20diverse.%20The%20ancestral%20karyotype%20of%20Pholcus%20contained%20five%20or%20six%20terminal%20NORs%20including%20three%20X%20chromosome-linked%20loci.%20The%20number%20of%20NORs%20has%20been%20frequently%20reduced%20during%20evolution.%20In%20the%20Macaronesian%20clade%2C%20there%20is%20only%20a%20single%20NOR-bearing%20pair.%20Sex%20chromosome-linked%20NORs%20are%20lost%20in%20Madeiran%20species%20and%20in%20P.%20creticus.%20Our%20study%20revealed%20two%20cytotypes%20in%20the%20synanthropic%20species%20P.%20phalan-gioides%20%28Madeiran%20and%20Czech%29%2C%20which%20differ%20by%20their%20NOR%20pattern%20and%20chromosome%20morphology.%20In%20the%20Czech%20cytotype%2C%20the%20large%20acrocentric%20pair%20was%20transformed%20into%20a%20biarmed%20pair%20by%20pericentric%20inversion.%22%2C%22date%22%3A%222022%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3897%5C%2Fcompcytogen.v16.i4.85059%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221993-0771%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%226T3MEUGG%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%22FH8RUNT7%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Bl%5Cu00e1ha%20et%20al.%22%2C%22parsedDate%22%3A%222022%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BBl%26%23xE1%3Bha%2C%20J.%3B%20Sk%26%23xE1%3Blov%26%23xE1%3B%2C%20T.%3B%20Kalouskov%26%23xE1%3B%2C%20B.%3B%20Skorepa%2C%20O.%3B%20Cmunt%2C%20D.%3B%20Grob%26%23xE1%3Brov%26%23xE1%3B%2C%20V.%3B%20Pazicky%2C%20S.%3B%20Pol%26%23xE1%3Bchov%26%23xE1%3B%2C%20E.%3B%20Abreu%2C%20C.%3B%20Str%26%23xE1%3Bnsky%2C%20J.%3B%20Koval%2C%20T.%3B%20Duskov%26%23xE1%3B%2C%20J.%3B%20Zhao%2C%20Y.%20G.%3B%20Harlos%2C%20K.%3B%20Hasek%2C%20J.%3B%20Dohn%26%23xE1%3Blek%2C%20J.%3B%20Vanek%2C%20O.%20%26lt%3Bb%26gt%3BStructure%20of%20the%20Human%20NK%20Cell%20NKR-P1%3ALLT1%20Receptor%3ALigand%20Complex%20Reveals%20Clustering%20in%20the%20Immune%20Synapse%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BNATURE%20COMMUNICATIONS%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2022%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B13%26lt%3B%5C%2Fi%26gt%3B%20%281%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-022-32577-6%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-022-32577-6%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Structure%20of%20the%20human%20NK%20cell%20NKR-P1%3ALLT1%20receptor%3Aligand%20complex%20reveals%20clustering%20in%20the%20immune%20synapse%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Bl%5Cu00e1ha%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%22%2C%22lastName%22%3A%22Sk%5Cu00e1lov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%22%2C%22lastName%22%3A%22Kalouskov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Skorepa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Cmunt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Grob%5Cu00e1rov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Pazicky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Pol%5Cu00e1chov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%22%2C%22lastName%22%3A%22Abreu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Str%5Cu00e1nsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%22%2C%22lastName%22%3A%22Koval%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Duskov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Y.%20G.%22%2C%22lastName%22%3A%22Zhao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Harlos%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Hasek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Dohn%5Cu00e1lek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Vanek%22%7D%5D%2C%22abstractNote%22%3A%22NKR-P1%20is%20an%20inhibitory%20receptor%20on%20the%20surface%20of%20natural%20killer%20cells%2C%20and%20its%20engagement%20with%20the%20ligand%20LLT1%20on%20activated%20monocytes%20and%20B%20cells%20triggers%20NK%20cell%20self-tolerance%20and%20other%20immunological%20processes.%20Here%20authors%20set%20up%20a%20comprehensive%2C%20structure-based%20model%20of%20NKR-P1-LLT1%20interaction%20that%20involves%20NKR-P1%20homodimer%20formation%20and%20subsequent%20bridging%20of%20two%20LLT1%20molecules.%20Signaling%20by%20the%20human%20C-type%20lectin-like%20receptor%2C%20natural%20killer%20%28NK%29%20cell%20inhibitory%20receptor%20NKR-P1%2C%20has%20a%20critical%20role%20in%20many%20immune-related%20diseases%20and%20cancer.%20C-type%20lectin-like%20receptors%20have%20weak%20affinities%20to%20their%20ligands%3B%20therefore%2C%20setting%20up%20a%20comprehensive%20model%20of%20NKR-P1-LLT1%20interactions%20that%20considers%20the%20natural%20state%20of%20the%20receptor%20on%20the%20cell%20surface%20is%20necessary%20to%20understand%20its%20functions.%20Here%20we%20report%20the%20crystal%20structures%20of%20the%20NKR-P1%20and%20NKR-P1%3ALLT1%20complexes%2C%20which%20provides%20evidence%20that%20NKR-P1%20forms%20homodimers%20in%20an%20unexpected%20arrangement%20to%20enable%20LLT1%20binding%20in%20two%20modes%2C%20bridging%20two%20LLT1%20molecules.%20These%20interaction%20clusters%20are%20suggestive%20of%20an%20inhibitory%20immune%20synapse.%20By%20observing%20the%20formation%20of%20these%20clusters%20in%20solution%20using%20SEC-SAXS%20analysis%2C%20by%20dSTORM%20super-resolution%20microscopy%20on%20the%20cell%20surface%2C%20and%20by%20following%20their%20role%20in%20receptor%20signaling%20with%20freshly%20isolated%20NK%20cells%2C%20we%20show%20that%20only%20the%20ligation%20of%20both%20LLT1%20binding%20interfaces%20leads%20to%20effective%20NKR-P1%20inhibitory%20signaling.%20In%20summary%2C%20our%20findings%20collectively%20support%20a%20model%20of%20NKR-P1%3ALLT1%20clustering%2C%20which%20allows%20the%20interacting%20proteins%20to%20overcome%20weak%20ligand-receptor%20affinity%20and%20to%20trigger%20signal%20transduction%20upon%20cellular%20contact%20in%20the%20immune%20synapse.%22%2C%22date%22%3A%222022%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-022-32577-6%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%222041-1723%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%226T3MEUGG%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%22AGS6NPRA%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Elsnicova%20et%20al.%22%2C%22parsedDate%22%3A%222022%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BElsnicova%2C%20B.%3B%20Hornikova%2C%20D.%3B%20Tibenska%2C%20V.%3B%20Kolar%2C%20D.%3B%20Tlapakova%2C%20T.%3B%20Schmid%2C%20B.%3B%20Mallek%2C%20M.%3B%20Eggers%2C%20B.%3B%20Schl%26%23xF6%3Btzer-Schrehardt%2C%20U.%3B%20Peeva%2C%20V.%3B%20Berwanger%2C%20C.%3B%20Eberhard%2C%20B.%3B%20Durmus%2C%20H.%3B%20Schultheis%2C%20D.%3B%20Holtzhausen%2C%20C.%3B%20Schork%2C%20K.%3B%20Marcus%2C%20K.%3B%20Jordan%2C%20J.%3B%20L%26%23xFC%3Bcke%2C%20T.%3B%20Ven%2C%20P.%20F.%20M.%20van%20der%3B%20Schr%26%23xF6%3Bder%2C%20R.%3B%20Clemen%2C%20C.%20S.%3B%20Zurmanova%2C%20J.%20M.%20%26lt%3Bb%26gt%3BDesmin%20Knock-Out%20Cardiomyopathy%3A%20A%20Heart%20on%20the%20Verge%20of%20Metabolic%20Crisis%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BINTERNATIONAL%20JOURNAL%20OF%20MOLECULAR%20SCIENCES%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2022%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B23%26lt%3B%5C%2Fi%26gt%3B%20%2819%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms231912020%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms231912020%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Desmin%20Knock-Out%20Cardiomyopathy%3A%20A%20Heart%20on%20the%20Verge%20of%20Metabolic%20Crisis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%22%2C%22lastName%22%3A%22Elsnicova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Hornikova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Tibenska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Kolar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%22%2C%22lastName%22%3A%22Tlapakova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%22%2C%22lastName%22%3A%22Schmid%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Mallek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%22%2C%22lastName%22%3A%22Eggers%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22U.%22%2C%22lastName%22%3A%22Schl%5Cu00f6tzer-Schrehardt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Peeva%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%22%2C%22lastName%22%3A%22Berwanger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%22%2C%22lastName%22%3A%22Eberhard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22H.%22%2C%22lastName%22%3A%22Durmus%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Schultheis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%22%2C%22lastName%22%3A%22Holtzhausen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Schork%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Marcus%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Jordan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%22%2C%22lastName%22%3A%22L%5Cu00fccke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%20F.%20M.%20van%20der%22%2C%22lastName%22%3A%22Ven%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Schr%5Cu00f6der%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20S.%22%2C%22lastName%22%3A%22Clemen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20M.%22%2C%22lastName%22%3A%22Zurmanova%22%7D%5D%2C%22abstractNote%22%3A%22Desmin%20mutations%20cause%20familial%20and%20sporadic%20cardiomyopathies.%20In%20addition%20to%20perturbing%20the%20contractile%20apparatus%2C%20both%20desmin%20deficiency%20and%20mutated%20desmin%20negatively%20impact%20mitochondria.%20Impaired%20myocardial%20metabolism%20secondary%20to%20mitochondrial%20defects%20could%20conceivably%20exacerbate%20cardiac%20contractile%20dysfunction.%20We%20performed%20metabolic%20myocardial%20phenotyping%20in%20left%20ventricular%20cardiac%20muscle%20tissue%20in%20desmin%20knock-out%20mice.%20Our%20analyses%20revealed%20decreased%20mitochondrial%20number%2C%20ultrastructural%20mitochondrial%20defects%2C%20and%20impaired%20mitochondria-related%20metabolic%20pathways%20including%20fatty%20acid%20transport%2C%20activation%2C%20and%20catabolism.%20Glucose%20transporter%201%20and%20hexokinase-1%20expression%20and%20hexokinase%20activity%20were%20increased.%20While%20mitochondrial%20creatine%20kinase%20expression%20was%20reduced%2C%20fetal%20creatine%20kinase%20expression%20was%20increased.%20Proteomic%20analysis%20revealed%20reduced%20expression%20of%20proteins%20involved%20in%20electron%20transport%20mainly%20of%20complexes%20I%20and%20II%2C%20oxidative%20phosphorylation%2C%20citrate%20cycle%2C%20beta-oxidation%20including%20auxiliary%20pathways%2C%20amino%20acid%20catabolism%2C%20and%20redox%20reactions%20and%20oxidative%20stress.%20Thus%2C%20desmin%20deficiency%20elicits%20a%20secondary%20cardiac%20mitochondriopathy%20with%20severely%20impaired%20oxidative%20phosphorylation%20and%20fatty%20and%20amino%20acid%20metabolism.%20Increased%20glucose%20utilization%20and%20fetal%20creatine%20kinase%20upregulation%20likely%20portray%20attempts%20to%20maintain%20myocardial%20energy%20supply.%20It%20may%20be%20prudent%20to%20avoid%20medications%20worsening%20mitochondrial%20function%20and%20other%20metabolic%20stressors.%20Therapeutic%20interventions%20for%20mitochondriopathies%20might%20also%20improve%20the%20metabolic%20condition%20in%20desmin%20deficient%20hearts.%22%2C%22date%22%3A%222022%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3390%5C%2Fijms231912020%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221661-6596%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%226T3MEUGG%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%5D%7D
1.
Batelka, J.; Prokop, J.; Beutel, R. G. Systematic Position of Ptilophorus Dufourii Inferred from Its Primary Larva, with Notes on Ptilophorinae (Coleoptera: Ripiphoridae). Ann. Zool. 2022, 72 (4), 805–826. https://doi.org/10.3161/00034541ANZ2022.72.4.004.
1.
Pilátová, J.; Pánek, T.; Oborník, M.; Čepička, I.; Mojzeš, P. Revisiting Biocrystallization: Purine Crystalline Inclusions Are Widespread in Eukaryotes. ISME J 2022, 16 (9), 2290–2294. https://doi.org/10.1038/s41396-022-01264-1.
1.
Mendez-Sanchez, D.; Pomahac, O.; Rotterova, J.; Bourland, W.; Cepicka, I. Diversity and Phylogenetic Position of Bothrostoma Stokes, 1887 (Ciliophora: Metopida), with Description of Four New Species. Protist 2022, 173 (4), 125887. https://doi.org/10.1016/j.protis.2022.125887.
1.
Glassnerova, K.; Sklenar, F.; Jurjevic, Z.; Houbraken, J.; Yaguchi, T.; Visagie, C. M.; Gene, J.; Siqueira, J. P. Z.; Kubatova, A.; Kolarik, M.; Hubka, V. A Monograph of Aspergillus Section Candidi. Stud. Mycol. 2022, No. 102, 1–51. https://doi.org/10.3114/sim.2022.102.01.
1.
Berankova, T.; Bucek, A.; Bourguignon, T.; Arias, J. R.; Akama, P. D.; Sillam-Dusses, D.; Sobotnik, J. The Ultrastructure of the Intramandibular Gland in Soldiers of the Termite Machadotermes Rigidus (Blattodea: Termitidae: Apicotermitinae). Arthropod Struct. Dev. 2022, 67, 101136. https://doi.org/10.1016/j.asd.2021.101136.
1.
Nemcova, Y.; Faturova, J.; Kreidlova, J. The New Species of Mallomonas (Stramenopiles, Chrysophyceae, Synurales): Mallomonas Decora Sp. Nov. Nova Hedwigia 2022, 114 (1–2), 1–8. https://doi.org/10.1127/nova_hedwigia/2022/0677.
1.
Bellinvia, E.; García-González, J.; Cifrová, P.; Martinek, J.; Sikorová, L.; Havelková, L.; Schwarzerová, K. CRISPR-Cas9 Arabidopsis Mutants of Genes for ARPC1 and ARPC3 Subunits of ARP2/3 Complex Reveal Differential Roles of Complex Subunits. SCIENTIFIC REPORTS 2022, 12 (1). https://doi.org/10.1038/s41598-022-22982-8.
1.
Paces, J.; Knízková, K.; Tusková, L.; Grobárová, V.; Zadrazil, Z.; Boes, M.; Cerny, J. MHC II-EGFP Knock-in Mouse Model Is a Suitable Tool for Systems and Quantitative Immunology. IMMUNOLOGY LETTERS 2022, 251–252, 75–85. https://doi.org/10.1016/j.imlet.2022.10.007.
1.
Just, P.; Stáhlavsky, F.; Kovarík, F.; Stundlová, J. Tracking the Trends of Karyotype Differentiation in the Phylogenetic Context of Gint, a Scorpion Genus Endemic to the Horn of Africa (Scorpiones: Buthidae). ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY 2022, 196 (2), 885–901. https://doi.org/10.1093/zoolinnean/zlac049.
1.
Král, J.; Herrera, I. M. A.; Stáhlavsky, F.; Sadílek, D.; Pavelka, J.; Chatzaki, M.; Huber, B. A. Karyotype Differentiation and Male Meiosis in European Clades of the Spider Genus Pholcus (Araneae, Pholcidae). COMPARATIVE CYTOGENETICS 2022, 16 (4), 185–209. https://doi.org/10.3897/compcytogen.v16.i4.85059.
1.
Bláha, J.; Skálová, T.; Kalousková, B.; Skorepa, O.; Cmunt, D.; Grobárová, V.; Pazicky, S.; Poláchová, E.; Abreu, C.; Stránsky, J.; Koval, T.; Dusková, J.; Zhao, Y. G.; Harlos, K.; Hasek, J.; Dohnálek, J.; Vanek, O. Structure of the Human NK Cell NKR-P1:LLT1 Receptor:Ligand Complex Reveals Clustering in the Immune Synapse. NATURE COMMUNICATIONS 2022, 13 (1). https://doi.org/10.1038/s41467-022-32577-6.
1.
Elsnicova, B.; Hornikova, D.; Tibenska, V.; Kolar, D.; Tlapakova, T.; Schmid, B.; Mallek, M.; Eggers, B.; Schlötzer-Schrehardt, U.; Peeva, V.; Berwanger, C.; Eberhard, B.; Durmus, H.; Schultheis, D.; Holtzhausen, C.; Schork, K.; Marcus, K.; Jordan, J.; Lücke, T.; Ven, P. F. M. van der; Schröder, R.; Clemen, C. S.; Zurmanova, J. M. Desmin Knock-Out Cardiomyopathy: A Heart on the Verge of Metabolic Crisis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2022, 23 (19). https://doi.org/10.3390/ijms231912020.
2021
5891878
54H5F7AF
1
https://raw.githubusercontent.com/Schebique/vmcf-konfmi/refs/heads/main/vmcf-web-style.csl
50
date
desc
4983
https://web.natur.cuni.cz/sekce-bi/VMCF/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22SIRRN89H%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Markova%20et%20al.%22%2C%22parsedDate%22%3A%222021-11-01%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BMarkova%2C%20V.%3B%20Hejnova%2C%20L.%3B%20Benda%2C%20A.%3B%20Novotny%2C%20J.%3B%20Melkes%2C%20B.%20%26lt%3Bb%26gt%3B%26%23x3B2%3B-Arrestin%201%20and%202%20Similarly%20Influence%20%26%23x3BC%3B-Opioid%20Receptor%20Mobility%20and%20Distinctly%20Modulate%20Adenylyl%20Cyclase%20Activity%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BCellular%20Signalling%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B87%26lt%3B%5C%2Fi%26gt%3B%2C%20110124.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.cellsig.2021.110124%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.cellsig.2021.110124%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22%5Cu03b2-Arrestin%201%20and%202%20similarly%20influence%20%5Cu03bc-opioid%20receptor%20mobility%20and%20distinctly%20modulate%20adenylyl%20cyclase%20activity%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vendula%22%2C%22lastName%22%3A%22Markova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucie%22%2C%22lastName%22%3A%22Hejnova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ales%22%2C%22lastName%22%3A%22Benda%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jiri%22%2C%22lastName%22%3A%22Novotny%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Barbora%22%2C%22lastName%22%3A%22Melkes%22%7D%5D%2C%22abstractNote%22%3A%22%5Cu03b2-Arrestins%20are%20known%20to%20play%20a%20crucial%20role%20in%20GPCR-mediated%20transmembrane%20signaling%20processes.%20However%2C%20there%20are%20still%20many%20unanswered%20questions%2C%20especially%20those%20concerning%20the%20presumed%20similarities%20and%20differences%20of%20%5Cu03b2-arrestin%20isoforms.%20Here%2C%20we%20examined%20the%20roles%20of%20%5Cu03b2-arrestin%201%20and%20%5Cu03b2-arrestin%202%20at%20different%20levels%20of%20%5Cu03bc-opioid%20receptor%20%28MOR%29-regulated%20signaling%2C%20including%20MOR%20mobility%2C%20internalization%20of%20MORs%2C%20and%20adenylyl%20cyclase%20%28AC%29%20activity.%20For%20this%20purpose%2C%20na%5Cu00efve%20HEK293%20cells%20or%20HEK293%20cells%20stably%20expressing%20YFP-tagged%20MOR%20were%20transfected%20with%20appropriate%20siRNAs%20to%20block%20in%20a%20specific%20way%20the%20expression%20of%20%5Cu03b2-arrestin%201%20or%20%5Cu03b2-arrestin%202.%20We%20did%20not%20find%20any%20significant%20differences%20in%20the%20ability%20of%20%5Cu03b2-arrestin%20isoforms%20to%20influence%20the%20lateral%20mobility%20of%20MORs%20in%20the%20plasma%20membrane.%20Using%20FRAP%20and%20line-scan%20FCS%2C%20we%20observed%20that%20knockdown%20of%20both%20%5Cu03b2-arrestins%20similarly%20increased%20MOR%20lateral%20mobility%20and%20diminished%20the%20ability%20of%20DAMGO%20and%20endomorphin-2%2C%20respectively%2C%20to%20enhance%20and%20slow%20down%20receptor%20diffusion%20kinetics.%20However%2C%20%5Cu03b2-arrestin%201%20and%20%5Cu03b2-arrestin%202%20diversely%20affected%20the%20process%20of%20agonist-induced%20MOR%20endocytosis%20and%20exhibited%20distinct%20modulatory%20effects%20on%20AC%20function.%20Knockdown%20of%20%5Cu03b2-arrestin%201%2C%20in%20contrast%20to%20%5Cu03b2-arrestin%202%2C%20more%20effectively%20suppressed%20forskolin-stimulated%20AC%20activity%20and%20prevented%20the%20ability%20of%20activated-MORs%20to%20inhibit%20the%20enzyme%20activity.%20Moreover%2C%20we%20have%20demonstrated%20for%20the%20first%20time%20that%20%5Cu03b2-arrestin%201%2C%20and%20partially%20%5Cu03b2-arrestin%202%2C%20may%20somehow%20interact%20with%20AC%20and%20that%20this%20interaction%20is%20strongly%20supported%20by%20the%20enzyme%20activation.%20These%20data%20provide%20new%20insights%20into%20the%20functioning%20of%20%5Cu03b2-arrestin%20isoforms%20and%20their%20distinct%20roles%20in%20GPCR-mediated%20signaling.%22%2C%22date%22%3A%222021-11-01%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.cellsig.2021.110124%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.sciencedirect.com%5C%2Fscience%5C%2Farticle%5C%2Fpii%5C%2FS0898656821002138%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220898-6568%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-03-07T10%3A11%3A24Z%22%7D%7D%2C%7B%22key%22%3A%22MCTRWJD2%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hoboth%20et%20al.%22%2C%22parsedDate%22%3A%222021-07%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BHoboth%2C%20P.%3B%20Sztacho%2C%20M.%3B%20Sebesta%2C%20O.%3B%20Hozak%2C%20P.%20%26lt%3Bb%26gt%3BUnraveling%20the%20Phospholipid%20Identity%20of%20the%20Gene%20Expression%20Compartments%20by%20Single%20Molecule%20Localization%20Microscopy%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BEur.%20Biophys.%20J.%20Biophys.%20Lett.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B50%26lt%3B%5C%2Fi%26gt%3B%20%28SUPPL%201%29%2C%20183%26%23x2013%3B183.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Unraveling%20the%20phospholipid%20identity%20of%20the%20gene%20expression%20compartments%20by%20single%20molecule%20localization%20microscopy%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Hoboth%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22Sztacho%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ondrej%22%2C%22lastName%22%3A%22Sebesta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pavel%22%2C%22lastName%22%3A%22Hozak%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%22JUL%202021%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%22%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220175-7571%2C%201432-1017%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A34Z%22%7D%7D%2C%7B%22key%22%3A%2297DZ7GEK%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Olenici%20and%20Fodor%22%2C%22parsedDate%22%3A%222021%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BOlenici%2C%20N.%3B%20Fodor%2C%20E.%20%26lt%3Bb%26gt%3BThe%20Diversity%20of%20Saproxylic%20Beetles%26%23x2019%3B%20Community%20from%20the%20Natural%20Reserve%20Voievodeasa%20Forest%2C%20North-Eastern%20Romania%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BAnn.%20For.%20Res.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B64%26lt%3B%5C%2Fi%26gt%3B%20%281%29%2C%2031%26%23x2013%3B60.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.15287%5C%2Fafr.2021.2144%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.15287%5C%2Fafr.2021.2144%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20diversity%20of%20saproxylic%20beetles%27%20community%20from%20the%20Natural%20Reserve%20Voievodeasa%20Forest%2C%20North-Eastern%20Romania%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicolai%22%2C%22lastName%22%3A%22Olenici%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ecaterina%22%2C%22lastName%22%3A%22Fodor%22%7D%5D%2C%22abstractNote%22%3A%22Nature%20reserves%20harbour%20considerable%20richness%20and%20diversity%20of%20saproxylic%20organisms%20since%20dead%20wood%20is%20preserved%20in%20situ%2C%20this%20being%20also%20the%20case%20of%20Voivodeasa%20beech-%20spruce-fir%20forest%20in%20North-Eastern%20Romania%2C%20the%20area%20investigated%20under%20the%20present%20research.%20Flight%20interception%20traps%20were%20employed%20to%20capture%20insects%20during%20a%20vegetation%20season%20with%20the%20goal%20to%20characterize%20saproxylic%20Coleoptera%20community%20in%20terms%20of%20diversity%20and%20several%20other%20structural%20features.%20Among%20the%20captured%20insects%2C%20the%20majority%20pertained%20to%20obligate%20saproxylic%20species%20%28217%20species%29.%20However%2C%20the%20unexpected%20high%20species%20richness%20corresponded%20to%20an%20area%20with%20modest%20representation%20of%20deadwood%20due%20to%20previous%20status%20of%20commercial%20forest.%20The%20identified%20beetles%20were%20members%20of%20different%20habitat-guilds%20depending%20on%20what%20type%20of%20substrate%20they%20colonized%3A%20recently%20dead%20wood%20%28%2023%25%29%2C%20decomposed%20dead%20wood%20%2841%25%29%2C%20wood%20inhabiting%20fungi%20%2834%25%29%20and%20treehollow%20detritus%20%28%202%25%29.%20According%20to%20their%20trophic%20position%2C%20the%20identified%20saproxylic%20beetles%20pertained%20to%20the%20following%20guilds%3A%20xylophagous%20%28%2040%25%29%2C%20mycetophagous%20%2839%25%29%2C%20predatory%20%2814%25%29%2C%20and%20species%20relying%20on%20other%20food%20resources.%20The%20observed%20richness%20corresponded%20to%20the%20case%20of%20hyperdiverse%20communities%20where%20sampling%20never%20leads%20to%20the%20stabilization%20of%20species%20richness%20under%20a%20realistic%20sampling%20scheme.%20The%20diversity%20profiles%20constructed%20on%20Shannon%2C%20Gini-Simpson%2C%20Berger-Parker%20and%20evenness%20indices%20for%20the%20pooled%20inventory%20and%20for%20separate%20samples%20across%20the%20vegetation%20season%20indicated%20the%20aggregated%20saproxylic%20community%20as%20highly%20diverse%20and%20highly%20uneven%2C%20with%20rich%20representation%20of%20rare%20species%2C%20dominated%20by%20few%20abundant%20species.%20We%20assembled%20four%20bipartite%2C%20unweighted%2C%20and%20undirected%20networks%20to%20approach%20the%20temporal%20changes%20across%20the%20sampling%20period%20extended%20over%20one%20vegetation%20season.%20The%20topology%20of%20beetles%26%23039%3B%20community%20and%20of%20the%20three%20main%20trophic%20guilds%20%28%20xylophagous%2C%20mycetophagous%20and%20predatory%29%20networks%20linked%20to%20time%20sequences%20are%20characterized%20by%20high%20connectance%2C%20high%20nestedness%20and%20modularity%2C%20with%20the%20exception%20of%20the%20mycetophagous%20sub-network%20not%20displaying%20significant%20modularity.%20Among%20the%20identified%20species%2C%2013%25%20indicate%20high%20degree%20of%20naturalness%20of%20the%20Voievodeasa%20forest.%2062%20of%20the%20identified%20species%20are%20included%20in%20the%20Red%20List%20of%20European%20Saproxylic%20Beetles%20of%20which%20five%20are%20near%20threatened%20%28Protaetia%20fieberi%2C%20Cucujus%20cinnaberinus%2C%20Crepidophorus%20mutilatus%2C%20Ceruchus%20chrysomelinus%2C%20Prostomis%20mandibularis%29%2C%20Ischnodes%20sanguinolentus%20is%20vulnerable%20and%20Rhysodes%20sulcatus%20is%20an%20endangered%20species.%20During%20the%20study%2C%20two%20Coleoptera%20species%2C%20new%20for%20Romanian%20insect%20fauna%20were%20identified%3A%20Denticollis%20interpositus%20Roubal%2C%201941%20and%20Hylis%20procerulus%20%28Mannerheim%201823%29.%22%2C%22date%22%3A%222021%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.15287%5C%2Fafr.2021.2144%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221844-8135%2C%202065-2445%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A24%3A41Z%22%7D%7D%2C%7B%22key%22%3A%223DWKWKI6%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22St%5Cu00e1hlavsky%20et%20al.%22%2C%22parsedDate%22%3A%222021%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BSt%26%23xE1%3Bhlavsky%2C%20F.%3B%20Kovar%26%23xED%3Bk%2C%20F.%3B%20Stockmann%2C%20M.%3B%20Opatova%2C%20V.%20%26lt%3Bb%26gt%3BKaryotype%20Evolution%20and%20Preliminary%20Molecular%20Assessment%20of%20Genera%20in%20the%20Family%20Scorpiopidae%20%28Arachnida%3A%20Scorpiones%29%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BZOOLOGY%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B144%26lt%3B%5C%2Fi%26gt%3B.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.zool.2020.125882%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.zool.2020.125882%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Karyotype%20evolution%20and%20preliminary%20molecular%20assessment%20of%20genera%20in%20the%20family%20Scorpiopidae%20%28Arachnida%3A%20Scorpiones%29%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22St%5Cu00e1hlavsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22Kovar%5Cu00edk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Stockmann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Opatova%22%7D%5D%2C%22abstractNote%22%3A%22The%20scorpions%20represent%20an%20ancient%20and%20morphologically%20conserved%20order%20of%20arachnids.%20Despite%20that%2C%20their%20karyotypes%20may%20differ%20considerably%20even%20among%20closely%20related%20species.%20In%20this%20study%2C%20we%20identify%20the%20trends%20of%20the%20karyotype%20evolution%20in%20the%20family%20Scorpiopidae%20based%20on%20integrating%20cytogenetic%20data%20and%20multi-locus%20molecular%20phylogenetic%20approaches.%20We%20detected%20considerable%20variability%20in%20diploid%20numbers%20of%20chromosomes%20%28from%2048%20to%20147%29%2C%2018S%20rRNA%20gene%20cluster%20positions%20%28from%20terminal%20to%20pericentromeric%29%20at%20the%20interspecific%20level.%20Moreover%2C%20we%20identified%20independent%20fusions%2C%20fissions%20and%20inversions%20in%20the%20evolution%20of%20the%20family%20Scorpiopidae%2C%20leading%20to%20a%20remarkable%20diversification%20of%20the%20karyotypes.%20The%20dynamic%20system%20of%20the%20karyotype%20changes%20in%20this%20group%20is%20further%20documented%20by%20the%20presence%20of%20interstitial%20telomeric%20sequences%20%28ITS%29%20in%20two%20species.%20The%20cytogenetic%20differences%20observed%20among%20the%20analyzed%20species%20highlight%20the%20potential%20of%20this%20type%20of%20data%20for%20species-level%20taxonomy%20in%20scorpion%20lineages%20with%20monocentric%20chromosomes.%20Additionally%2C%20the%20results%20of%20our%20phylogenetic%20analyses%20support%20the%20monophyly%20of%20the%20family%20Scorpiopidae%2C%20but%20rendered%20several%20genera%20para-%20or%20polyphyletic.%22%2C%22date%22%3A%222021%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.zool.2020.125882%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220944-2006%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%22URFVNFF8%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hoboth%20et%20al.%22%2C%22parsedDate%22%3A%222021%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BHoboth%2C%20P.%3B%20Sebesta%2C%20O.%3B%20Sztacho%2C%20M.%3B%20Castano%2C%20E.%3B%20Hoz%26%23xE1%3Bk%2C%20P.%20%26lt%3Bb%26gt%3BDual-Color%20dSTORM%20Imaging%20and%20ThunderSTORM%20Image%20Reconstruction%20and%20Analysis%20to%20Study%20the%20Spatial%20Organization%20of%20the%20Nuclear%20Phosphatidylinositol%20Phosphates%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BMETHODSX%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B8%26lt%3B%5C%2Fi%26gt%3B.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.mex.2021.101372%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.mex.2021.101372%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Dual-color%20dSTORM%20imaging%20and%20ThunderSTORM%20image%20reconstruction%20and%20analysis%20to%20study%20the%20spatial%20organization%20of%20the%20nuclear%20phosphatidylinositol%20phosphates%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Hoboth%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Sebesta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Sztacho%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Castano%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Hoz%5Cu00e1k%22%7D%5D%2C%22abstractNote%22%3A%22Single%20molecule%20localization%20microscopy%20%28SMLM%29%20provided%20an%20unprecedented%20insight%20into%20the%20sub-nuclear%20organization%20of%20proteins%20and%20nucleic%20acids%20but%20apart%20from%20the%20nuclear%20envelope%20the%20role%20of%20the%20nuclear%20lipids%20in%20the%20functional%20organization%20of%20the%20cell%20nucleus%20was%20less%20studied.%20Nevertheless%2C%20nuclear%20lipids%20and%20specifically%20phosphatidylinositol%20phosphates%20%28PIPs%29%20play%20increasingly%20evident%20roles%20in%20gene%20expression.%20Therefore%2C%20here%20we%20provide%20the%20SMLM-based%20approach%20for%20the%20quantitative%20evaluation%20of%20the%20nuclear%20PIPs%20distribution%20while%20preserving%20the%20context%20of%20nuclear%20architecture.%20Specifically%2C%20on%20the%20example%20of%20phosphatidylinositol%204%2C5-bisphosphate%20%28PIP2%29%20we%20have%3A%20Implemented%20and%20optimized%20the%20dual-color%20dSTORM%20imaging%20of%20nuclear%20PIP2.%20Customized%20the%20Nearest%20Neighbor%20Distance%20analysis%20using%20ImageJ2%20plug-in%20ThunderSTORM%20to%20quantitatively%20evaluate%20the%20spatial%20distribution%20of%20nuclear%20PIP2.%20Developed%20an%20ImageJ2%20tool%20for%20the%20visualization%20of%20the%20Nearest%20Neighbor%20Distance%20analysis%20results%20in%20cellulo.%20Our%20customization%20of%20the%20dual-color%20dSTORM%20imaging%20and%20quantitative%20analysis%20provide%20a%20tool%20that%20is%20independent%20of%20but%20complementary%20to%20the%20biochemical%20and%20lipidomic%20analyses%20of%20the%20nuclear%20PIPs.%20Contrary%20to%20the%20biochemical%20and%20lipidomic%20analyses%2C%20the%20advantage%20of%20our%20analysis%20is%20that%20it%20preserves%20the%20spatial%20context%20of%20the%20nuclear%20PIP%20distribution.%20%28C%29%202021%20The%20Authors.%20Published%20by%20Elsevier%20B.V.%22%2C%22date%22%3A%222021%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.mex.2021.101372%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%222215-0161%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%227JZW9ARZ%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Morgan%20et%20al.%22%2C%22parsedDate%22%3A%222021%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BMorgan%2C%20E.%20J.%3B%20Certner%2C%20M.%3B%20Lucanov%26%23xE1%3B%2C%20M.%3B%20Deniz%2C%20U.%3B%20Kub%26%23xED%3Bkov%26%23xE1%3B%2C%20K.%3B%20Venon%2C%20A.%3B%20Kovar%26%23xED%3Bk%2C%20O.%3B%20Placette%2C%20C.%20L.%3B%20Kol%26%23xE1%3Br%2C%20F.%20%26lt%3Bb%26gt%3BDisentangling%20the%20Components%20of%20Triploid%20Block%20and%20Its%20Fitness%20Consequences%20in%20Natural%20Diploid-Tetraploid%20Contact%20Zones%20of%20%26lt%3Bi%26gt%3BArabidopsis%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bi%26gt%3BArenosa%26lt%3B%5C%2Fi%26gt%3B%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BNEW%20PHYTOLOGIST%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B232%26lt%3B%5C%2Fi%26gt%3B%20%283%29%2C%201449%26%23x2013%3B1462.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fnph.17357%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fnph.17357%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Disentangling%20the%20components%20of%20triploid%20block%20and%20its%20fitness%20consequences%20in%20natural%20diploid-tetraploid%20contact%20zones%20of%20%3Ci%3EArabidopsis%3C%5C%2Fi%3E%20%3Ci%3Earenosa%3C%5C%2Fi%3E%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20J.%22%2C%22lastName%22%3A%22Morgan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Certner%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Lucanov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22U.%22%2C%22lastName%22%3A%22Deniz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Kub%5Cu00edkov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Venon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Kovar%5Cu00edk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20L.%22%2C%22lastName%22%3A%22Placette%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22Kol%5Cu00e1r%22%7D%5D%2C%22abstractNote%22%3A%22Hybrid%20seed%20inviability%20%28HSI%29%20is%20an%20important%20mechanism%20of%20reproductive%20isolation%20and%20speciation.%20HSI%20varies%20in%20strength%20among%20populations%20of%20diploid%20species%20but%20it%20remains%20to%20be%20tested%20whether%20similar%20processes%20affect%20natural%20variation%20in%20HSI%20within%20ploidy-variable%20species%20%28triploid%20block%29.%20Here%20we%20used%20extensive%20endosperm%2C%20seed%20and%20F-1-hybrid%20phenotyping%20to%20explore%20HSI%20variation%20within%20a%20diploid-autotetraploid%20species.%20By%20leveraging%2012%20population%20pairs%20from%20three%20ploidy%20contact%20zones%2C%20we%20tested%20for%20the%20effect%20of%20interploidy%20crossing%20direction%20%28parent%20of%20origin%29%2C%20ploidy%20divergence%20and%20spatial%20arrangement%20in%20shaping%20reproductive%20barriers%20in%20a%20naturally%20relevant%20context.%20We%20detected%20strong%20parent-of-origin%20effects%20on%20endosperm%20development%2C%20F-1%20germination%20and%20survival%2C%20which%20was%20also%20reflected%20in%20the%20rates%20of%20triploid%20formation%20in%20the%20field.%20Endosperm%20cellularization%20failure%20was%20least%20severe%20and%20F-1-hybrid%20performance%20was%20slightly%20better%20in%20the%20primary%20contact%20zone%2C%20with%20genetically%20closest%20diploid%20and%20tetraploid%20lineages.%20We%20demonstrated%20overall%20strong%20parent-of-origin%20effects%20on%20HSI%20in%20a%20ploidy%20variable%20species%2C%20which%20translate%20to%20fitness%20effects%20and%20contribute%20to%20interploidy%20reproductive%20isolation%20in%20a%20natural%20context.%20Subtle%20intraspecific%20variation%20in%20these%20traits%20suggests%20the%20fitness%20consequences%20of%20HSI%20are%20predominantly%20a%20constitutive%20property%20of%20the%20species%20regardless%20of%20the%20evolutionary%20background%20of%20its%20populations.%22%2C%22date%22%3A%222021%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2Fnph.17357%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220028-646X%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%22GLCVLGWU%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Herrera%20et%20al.%22%2C%22parsedDate%22%3A%222021%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BHerrera%2C%20I.%20M.%20A.%3B%20Kr%26%23xE1%3Bl%2C%20J.%3B%20Pastuchov%26%23xE1%3B%2C%20M.%3B%20Forman%2C%20M.%3B%20Musilov%26%23xE1%3B%2C%20J.%3B%20Kor%26%23xED%3Bnkov%26%23xE1%3B%2C%20T.%3B%20St%26%23xE1%3Bhlavsky%2C%20F.%3B%20Zrzav%26%23xE1%3B%2C%20M.%3B%20Nguyen%2C%20P.%3B%20Just%2C%20P.%3B%20Haddad%2C%20C.%20R.%3B%20Hirman%2C%20M.%3B%20Koubov%26%23xE1%3B%2C%20M.%3B%20Sad%26%23xED%3Blek%2C%20D.%3B%20Huber%2C%20B.%20A.%20%26lt%3Bb%26gt%3BEvolutionary%20Pattern%20of%20Karyotypes%20and%20Meiosis%20in%20Pholcid%20Spiders%20%28Araneae%3A%20Pholcidae%29%3A%20Implications%20for%20Reconstructing%20Chromosome%20Evolution%20of%20Araneomorph%20Spiders%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BBMC%20ECOLOGY%20AND%20EVOLUTION%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B21%26lt%3B%5C%2Fi%26gt%3B%20%281%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs12862-021-01750-8%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs12862-021-01750-8%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Evolutionary%20pattern%20of%20karyotypes%20and%20meiosis%20in%20pholcid%20spiders%20%28Araneae%3A%20Pholcidae%29%3A%20implications%20for%20reconstructing%20chromosome%20evolution%20of%20araneomorph%20spiders%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22I.%20M.%20A.%22%2C%22lastName%22%3A%22Herrera%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Kr%5Cu00e1l%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Pastuchov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Forman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Musilov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%22%2C%22lastName%22%3A%22Kor%5Cu00ednkov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22St%5Cu00e1hlavsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Zrzav%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Nguyen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Just%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20R.%22%2C%22lastName%22%3A%22Haddad%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Hirman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Koubov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Sad%5Cu00edlek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20A.%22%2C%22lastName%22%3A%22Huber%22%7D%5D%2C%22abstractNote%22%3A%22Background%20Despite%20progress%20in%20genomic%20analysis%20of%20spiders%2C%20their%20chromosome%20evolution%20is%20not%20satisfactorily%20understood.%20Most%20information%20on%20spider%20chromosomes%20concerns%20the%20most%20diversified%20clade%2C%20entelegyne%20araneomorphs.%20Other%20clades%20are%20far%20less%20studied.%20Our%20study%20focused%20on%20haplogyne%20araneomorphs%2C%20which%20are%20remarkable%20for%20their%20unusual%20sex%20chromosome%20systems%20and%20for%20the%20co-evolution%20of%20sex%20chromosomes%20and%20nucleolus%20organizer%20regions%20%28NORs%29%3B%20some%20haplogynes%20exhibit%20holokinetic%20chromosomes.%20To%20trace%20the%20karyotype%20evolution%20of%20haplogynes%20on%20the%20family%20level%2C%20we%20analysed%20the%20number%20and%20morphology%20of%20chromosomes%2C%20sex%20chromosomes%2C%20NORs%2C%20and%20meiosis%20in%20pholcids%2C%20which%20are%20among%20the%20most%20diverse%20haplogyne%20families.%20The%20evolution%20of%20spider%20NORs%20is%20largely%20unknown.%20Results%20Our%20study%20is%20based%20on%20an%20extensive%20set%20of%20species%20representing%20all%20major%20pholcid%20clades.%20Pholcids%20exhibit%20a%20low%202n%20and%20predominance%20of%20biarmed%20chromosomes%2C%20which%20are%20typical%20haplogyne%20features.%20Sex%20chromosomes%20and%20NOR%20patterns%20of%20pholcids%20are%20diversified.%20We%20revealed%20six%20sex%20chromosome%20systems%20in%20pholcids%20%28X0%2C%20XY%2C%20X%281%29X%282%290%2C%20X%281%29X%282%29X%283%290%2C%20X1X2Y%2C%20and%20X1X2X3X4Y%29.%20The%20number%20of%20NOR%20loci%20ranges%20from%20one%20to%20nine.%20In%20some%20clades%2C%20NORs%20are%20also%20found%20on%20sex%20chromosomes.%20Conclusions%20The%20evolution%20of%20cytogenetic%20characters%20was%20largely%20derived%20from%20character%20mapping%20on%20a%20recently%20published%20molecular%20phylogeny%20of%20the%20family.%20Based%20on%20an%20extensive%20set%20of%20species%20and%20mapping%20of%20their%20characters%2C%20numerous%20conclusions%20regarding%20the%20karyotype%20evolution%20of%20pholcids%20and%20spiders%20can%20be%20drawn.%20Our%20results%20suggest%20frequent%20autosome-autosome%20and%20autosome-sex%20chromosome%20rearrangements%20during%20pholcid%20evolution.%20Such%20events%20have%20previously%20been%20attributed%20to%20the%20reproductive%20isolation%20of%20species.%20The%20peculiar%20X1X2Y%20system%20is%20probably%20ancestral%20for%20haplogynes.%20Chromosomes%20of%20the%20X1X2Y%20system%20differ%20considerably%20in%20their%20pattern%20of%20evolution.%20In%20some%20pholcid%20clades%2C%20the%20X1X2Y%20system%20has%20transformed%20into%20the%20X%281%29X%282%290%20or%20XY%20systems%2C%20and%20subsequently%20into%20the%20X0%20system.%20The%20X%281%29X%282%29X%283%290%20system%20of%20Smeringopus%20pallidus%20probably%20arose%20from%20the%20X%281%29X%282%290%20system%20by%20an%20X%20chromosome%20fission.%20The%20X1X2X3X4Y%20system%20of%20Kambiwa%20probably%20evolved%20from%20the%20X1X2Y%20system%20by%20integration%20of%20a%20chromosome%20pair.%20Nucleolus%20organizer%20regions%20have%20frequently%20expanded%20on%20sex%20chromosomes%2C%20most%20probably%20by%20ectopic%20recombination.%20Our%20data%20suggest%20the%20involvement%20of%20sex%20chromosome-linked%20NORs%20in%20achiasmatic%20pairing.%22%2C%22date%22%3A%222021%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1186%5C%2Fs12862-021-01750-8%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%222730-7182%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%22YHJVUP2X%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hoboth%20et%20al.%22%2C%22parsedDate%22%3A%222021%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BHoboth%2C%20P.%3B%20Sztacho%2C%20M.%3B%20Sebesta%2C%20O.%3B%20Sch%26%23xE4%3Btz%2C%20M.%3B%20Castano%2C%20E.%3B%20Hoz%26%23xE1%3Bk%2C%20P.%20%26lt%3Bb%26gt%3BNanoscale%20Mapping%20of%20Nuclear%20Phosphatidylinositol%20Phosphate%20Landscape%20by%20Dual-Color%20dSTORM%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BBIOCHIMICA%20ET%20BIOPHYSICA%20ACTA-MOLECULAR%20AND%20CELL%20BIOLOGY%20OF%20LIPIDS%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B1866%26lt%3B%5C%2Fi%26gt%3B%20%285%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.bbalip.2021.158890%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.bbalip.2021.158890%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Nanoscale%20mapping%20of%20nuclear%20phosphatidylinositol%20phosphate%20landscape%20by%20dual-color%20dSTORM%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Hoboth%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Sztacho%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Sebesta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Sch%5Cu00e4tz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Castano%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Hoz%5Cu00e1k%22%7D%5D%2C%22abstractNote%22%3A%22Current%20models%20of%20gene%20expression%2C%20which%20are%20based%20on%20single-molecule%20localization%20microscopy%2C%20acknowledge%20protein%20clustering%20and%20the%20formation%20of%20transcriptional%20condensates%20as%20a%20driving%20force%20of%20gene%20expression.%20However%2C%20these%20models%20largely%20omit%20the%20role%20of%20nuclear%20lipids%20and%20amongst%20them%20nuclear%20phosphatidylinositol%20phosphates%20%28PIPs%29%20in%20particular.%20Moreover%2C%20the%20precise%20distribution%20of%20nuclear%20PIPs%20in%20the%20functional%20sub-nuclear%20domains%20remains%20elusive.%20The%20direct%20stochastic%20optical%20reconstruction%20microscopy%20%28dSTORM%29%20provides%20an%20unprecedented%20resolution%20in%20biological%20imaging.%20Therefore%2C%20its%20use%20for%20imaging%20in%20the%20densely%20crowded%20cell%20nucleus%20is%20desired%20but%20also%20challenging.%20Here%20we%20present%20a%20dual-color%20dSTORM%20imaging%20and%20image%20analysis%20of%20nuclear%20PI%20%284%2C5%29P2%2C%20PI%283%2C4%29P2%20and%20PI%284%29P%20distribution%20while%20preserving%20the%20context%20of%20nuclear%20architecture.%20In%20the%20nucleoplasm%2C%20PI%284%2C5%29P2%20and%20PI%283%2C4%29P2%20co-pattern%20in%20close%20proximity%20with%20the%20subset%20of%20RNA%20polymerase%20II%20foci.%20PI%284%2C5%29P2%20is%20surrounded%20by%20fibrillarin%20in%20the%20nucleoli%20and%20all%20three%20PIPs%20are%20dispersed%20within%20the%20matrix%20formed%20by%20the%20nuclear%20speckle%20protein%20SON.%20PI%284%2C5%29P2%20is%20the%20most%20abundant%20nuclear%20PIP%2C%20while%20PI%284%29P%20is%20a%20precursor%20for%20the%20biosynthesis%20of%20PI%284%2C5%29P2%20and%20PI%283%2C4%29P2.%20Therefore%2C%20our%20data%20are%20relevant%20for%20the%20understanding%20the%20roles%20of%20nuclear%20PIPs%20and%20provide%20further%20evidence%20for%20the%20model%20in%20which%20nuclear%20PIPs%20represent%20a%20localization%20signal%20for%20the%20formation%20of%20lipo-ribonucleoprotein%20hubs%20in%20the%20nucleus.%20The%20discussed%20experimental%20pipeline%20is%20applicable%20for%20further%20functional%20studies%20on%20the%20role%20of%20other%20nuclear%20PIPs%20in%20the%20regulation%20of%20gene%20expression%20and%20beyond.%22%2C%22date%22%3A%222021%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.bbalip.2021.158890%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221388-1981%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%22CYQK3WWI%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Koll%5Cu00e1rov%5Cu00e1%20et%20al.%22%2C%22parsedDate%22%3A%222021%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BKoll%26%23xE1%3Brov%26%23xE1%3B%2C%20E.%3B%20Forero%2C%20A.%20B.%3B%20Cvrckov%26%23xE1%3B%2C%20F.%20%26lt%3Bb%26gt%3BThe%20%26lt%3Bi%26gt%3BArabidopsis%20Thaliana%26lt%3B%5C%2Fi%26gt%3B%20Class%20II%20Formin%20FH13%20Modulates%20Pollen%20Tube%20Growth%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BFRONTIERS%20IN%20PLANT%20SCIENCE%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B12%26lt%3B%5C%2Fi%26gt%3B.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffpls.2021.599961%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffpls.2021.599961%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20%3Ci%3EArabidopsis%20thaliana%3C%5C%2Fi%3E%20Class%20II%20Formin%20FH13%20Modulates%20Pollen%20Tube%20Growth%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Koll%5Cu00e1rov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20B.%22%2C%22lastName%22%3A%22Forero%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22Cvrckov%5Cu00e1%22%7D%5D%2C%22abstractNote%22%3A%22Formins%20are%20a%20large%2C%20evolutionarily%20conserved%20family%20of%20actin-nucleating%20proteins%20with%20additional%20roles%20in%20regulating%20microfilament%2C%20microtubule%2C%20and%20membrane%20dynamics.%20Angiosperm%20formins%2C%20expressed%20in%20both%20sporophytic%20and%20gametophytic%20tissues%2C%20can%20be%20divided%20into%20two%20subfamilies%2C%20Class%20I%20and%20Class%20II%2C%20each%20often%20exhibiting%20characteristic%20domain%20organization.%20Gametophytically%20expressed%20Class%20I%20formins%20have%20been%20documented%20to%20mediate%20plasma%20membrane-based%20actin%20assembly%20in%20pollen%20grains%20and%20pollen%20tubes%2C%20contributing%20to%20proper%20pollen%20germination%20and%20pollen%20tube%20tip%20growth%2C%20and%20a%20rice%20Class%20II%20formin%2C%20FH5%5C%2FRMD%2C%20has%20been%20proposed%20to%20act%20as%20a%20positive%20regulator%20of%20pollen%20tube%20growth%20based%20on%20mutant%20phenotype%20and%20overexpression%20data.%20Here%20we%20report%20functional%20characterization%20of%20the%20Arabidopsis%20thaliana%20pollen-expressed%20typical%20Class%20II%20formin%20FH13%20%28At5g58160%29.%20Consistent%20with%20published%20transcriptome%20data%2C%20live-cell%20imaging%20in%20transgenic%20plants%20expressing%20fluorescent%20protein-tagged%20FH13%20under%20the%20control%20of%20the%20FH13%20promoter%20revealed%20expression%20in%20pollen%20and%20pollen%20tubes%20with%20non-homogeneous%20signal%20distribution%20in%20pollen%20tube%20cytoplasm%2C%20suggesting%20that%20this%20formin%20functions%20in%20the%20male%20gametophyte.%20Surprisingly%2C%20fh13%20loss%20of%20function%20mutations%20do%20not%20affect%20plant%20fertility%20but%20result%20in%20stimulation%20of%20in%20vitro%20pollen%20tube%20growth%2C%20while%20tagged%20FH13%20overexpression%20inhibits%20pollen%20tube%20elongation.%20Pollen%20tubes%20of%20mutants%20expressing%20a%20fluorescent%20actin%20marker%20exhibited%20possible%20minor%20alterations%20of%20actin%20organization.%20Our%20results%20thus%20indicate%20that%20FH13%20controls%20or%20limits%20pollen%20tube%20growth%2C%20or%2C%20more%20generally%2C%20that%20typical%20Class%20II%20formins%20should%20be%20understood%20as%20modulators%20of%20pollen%20tube%20elongation%20rather%20than%20merely%20components%20of%20the%20molecular%20apparatus%20executing%20tip%20growth.%22%2C%22date%22%3A%222021%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3389%5C%2Ffpls.2021.599961%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221664-462X%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A33Z%22%7D%7D%2C%7B%22key%22%3A%228A4WUZ5L%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Tibenska%20et%20al.%22%2C%22parsedDate%22%3A%222021%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BTibenska%2C%20V.%3B%20Marvanova%2C%20A.%3B%20Elsnicova%2C%20B.%3B%20Hejnova%2C%20L.%3B%20Vebr%2C%20P.%3B%20Novotny%2C%20J.%3B%20Kolar%2C%20F.%3B%20Novakova%2C%20O.%3B%20Zurmanova%2C%20J.%20M.%20%26lt%3Bb%26gt%3BThe%20Cardioprotective%20Effect%20Persisting%20during%20Recovery%20from%20Cold%20Acclimation%20Is%20Mediated%20by%20the%20%26%23x3B2%3B%26lt%3Bsub%26gt%3B2%26lt%3B%5C%2Fsub%26gt%3B-Adrenoceptor%20Pathway%20and%20Akt%20Activation%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BJOURNAL%20OF%20APPLIED%20PHYSIOLOGY%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B130%26lt%3B%5C%2Fi%26gt%3B%20%283%29%2C%20746%26%23x2013%3B755.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1152%5C%2Fjapplphysiol.00756.2020%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1152%5C%2Fjapplphysiol.00756.2020%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20cardioprotective%20effect%20persisting%20during%20recovery%20from%20cold%20acclimation%20is%20mediated%20by%20the%20%5Cu03b2%3Csub%3E2%3C%5C%2Fsub%3E-adrenoceptor%20pathway%20and%20Akt%20activation%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Tibenska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Marvanova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%22%2C%22lastName%22%3A%22Elsnicova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Hejnova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Vebr%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Novotny%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22Kolar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Novakova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20M.%22%2C%22lastName%22%3A%22Zurmanova%22%7D%5D%2C%22abstractNote%22%3A%22The%20infarct%20size-limiting%20effect%20elicited%20by%20cold%20acclimation%20%28CA%29%20is%20accompanied%20by%20increased%20mitochondrial%20resistance%20and%20unaltered%20beta%281%29-adrenergic%20receptor%20%28AR%29%20signaling%20persisting%20for%202%20wk%20at%20room%20temperature.%20As%20the%20mechanism%20of%20CA-elicited%20cardioprotection%20is%20not%20fully%20understood%2C%20we%20examined%20the%20role%20of%20the%20salvage%20beta%282%29-AR%5C%2FG%28i%29%5C%2FAkt%20pathway.%20Male%20Wistar%20rats%20were%20exposed%20to%20CA%20%288%20degrees%20C%2C%205%20wk%29%2C%20whereas%20the%20recovery%20group%20%28CAR%29%20was%20kept%20at%2024%20degrees%20C%20for%20additional%202%20wk.%20We%20show%20that%20the%20total%20number%20of%20myocardial%20beta-ARs%20in%20the%20left%20ventricular%20myocardium%20did%20not%20change%20after%20CA%20but%20decreased%20after%20CAR.%20We%20confirmed%20the%20infarct%20size-limiting%20effect%20in%20both%20CA%20and%20CAR%20groups.%20Acute%20administration%20of%20beta%282%29-AR%20inhibitor%20ICI-118551%20abolished%20the%20protective%20effect%20in%20the%20CAR%20group%20but%20had%20no%20effect%20in%20the%20control%20and%20CA%20groups.%20The%20inhibitory%20G%28i%29alpha%281%5C%2F2%29%20and%20G%28i%29alpha%283%29%20proteins%20increased%20in%20the%20membrane%20fraction%20of%20the%20CAR%20group%2C%20and%20the%20phospho-Akt%20%28Ser%28473%29%29-to-Akt%20ratio%20also%20increased.%20Expression%2C%20phosphorylation%2C%20and%20mitochondrial%20location%20of%20the%20Akt%20target%20glycogen%20synthase%20kinase%20%28GSK-3%20beta%29%20were%20affected%20neither%20by%20CA%20nor%20by%20CAR.%20However%2C%20GSK-3%20beta%20translocated%20from%20the%20Z-disk%20to%20the%20H-zone%20after%20CA%2C%20and%20acquired%20its%20original%20location%20after%20CAR.%20Our%20data%20indicate%20that%20the%20cardioprotection%20observed%20after%20CAR%20is%20mediated%20by%20the%20beta%202-AR%5C%2FG%28i%29%20pathway%20and%20Akt%20activation.%20Further%20studies%20are%20needed%20to%20unravel%20downstream%20targets%20of%20the%20central%20regulators%20of%20the%20CA%20process%20and%20the%20downstream%20targets%20of%20the%20Akt%20protein%20after%20CAR.%20NEW%20%26amp%3B%20NOTEWORTHY%20Cardioprotective%20effect%20of%20cold%20acclimation%20and%20that%20persisting%20for%202%20wk%20after%20recovery%20engage%20in%20different%20mechanisms.%20The%20beta%282%29-adrenoceptor%5C%2FG%28i%29%20pathway%20and%20Akt%20are%20involved%20only%20in%20the%20mechanism%20of%20infarct%20size-limiting%20effect%20occurring%20during%20the%20recovery%20phase.%20GSK-3%20beta%20translocated%20from%20the%20Z-line%20to%20the%20H-zone%20of%20sarcomeres%20by%20cold%20acclimation%20returns%20back%20to%20the%20original%20position%20after%20the%20recovery%20phase.%20The%20results%20provide%20new%20insights%20potentially%20useful%20for%20the%20development%20of%20cardiac%20therapies.%22%2C%22date%22%3A%222021%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1152%5C%2Fjapplphysiol.00756.2020%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%228750-7587%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A33Z%22%7D%7D%2C%7B%22key%22%3A%22UQNNHQBD%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hoboth%20et%20al.%22%2C%22parsedDate%22%3A%222021%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BHoboth%2C%20P.%3B%20Sebesta%2C%20O.%3B%20Hoz%26%23xE1%3Bk%2C%20P.%20%26lt%3Bb%26gt%3BHow%20Single-Molecule%20Localization%20Microscopy%20Expanded%20Our%20Mechanistic%20Understanding%20of%20RNA%20Polymerase%20II%20Transcription%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BINTERNATIONAL%20JOURNAL%20OF%20MOLECULAR%20SCIENCES%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B22%26lt%3B%5C%2Fi%26gt%3B%20%2813%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms22136694%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms22136694%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22How%20Single-Molecule%20Localization%20Microscopy%20Expanded%20Our%20Mechanistic%20Understanding%20of%20RNA%20Polymerase%20II%20Transcription%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Hoboth%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Sebesta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Hoz%5Cu00e1k%22%7D%5D%2C%22abstractNote%22%3A%22Classical%20models%20of%20gene%20expression%20were%20built%20using%20genetics%20and%20biochemistry.%20Although%20these%20approaches%20are%20powerful%2C%20they%20have%20very%20limited%20consideration%20of%20the%20spatial%20and%20temporal%20organization%20of%20gene%20expression.%20Although%20the%20spatial%20organization%20and%20dynamics%20of%20RNA%20polymerase%20II%20%28RNAPII%29%20transcription%20machinery%20have%20fundamental%20functional%20consequences%20for%20gene%20expression%2C%20its%20detailed%20studies%20have%20been%20abrogated%20by%20the%20limits%20of%20classical%20light%20microscopy%20for%20a%20long%20time.%20The%20advent%20of%20super-resolution%20microscopy%20%28SRM%29%20techniques%20allowed%20for%20the%20visualization%20of%20the%20RNAPII%20transcription%20machinery%20with%20nanometer%20resolution%20and%20millisecond%20precision.%20In%20this%20review%2C%20we%20summarize%20the%20recent%20methodological%20advances%20in%20SRM%2C%20focus%20on%20its%20application%20for%20studies%20of%20the%20nanoscale%20organization%20in%20space%20and%20time%20of%20RNAPII%20transcription%2C%20and%20discuss%20its%20consequences%20for%20the%20mechanistic%20understanding%20of%20gene%20expression.%22%2C%22date%22%3A%222021%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3390%5C%2Fijms22136694%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221422-0067%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A33Z%22%7D%7D%2C%7B%22key%22%3A%22L7NT6SU3%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Palenikova%20et%20al.%22%2C%22parsedDate%22%3A%222021%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BPalenikova%2C%20V.%3B%20Frolikova%2C%20M.%3B%20Valaskova%2C%20E.%3B%20Postlerova%2C%20P.%3B%20Komrskova%2C%20K.%20%26lt%3Bb%26gt%3B%26%23x3B1%3BV%20Integrin%20Expression%20and%20Localization%20in%20Male%20Germ%20Cells%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BINTERNATIONAL%20JOURNAL%20OF%20MOLECULAR%20SCIENCES%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B22%26lt%3B%5C%2Fi%26gt%3B%20%2817%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms22179525%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms22179525%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22%5Cu03b1V%20Integrin%20Expression%20and%20Localization%20in%20Male%20Germ%20Cells%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Palenikova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Frolikova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Valaskova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Postlerova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Komrskova%22%7D%5D%2C%22abstractNote%22%3A%22Integrins%20are%20transmembrane%20receptors%20that%20facilitate%20cell%20adhesion%20and%20cell-extracellular%20matrix%20communication.%20They%20are%20involved%20in%20the%20sperm%20maturation%20including%20capacitation%20and%20gamete%20interaction%2C%20resulting%20in%20successful%20fertilization.%20alpha%20V%20integrin%20belongs%20to%20the%20integrin%20glycoprotein%20superfamily%2C%20and%20it%20is%20indispensable%20for%20physiological%20spermiogenesis%20and%20testosterone%20production.%20We%20targeted%20the%20gene%20and%20protein%20expression%20of%20the%20alpha%20V%20integrin%20subunit%20and%20described%20its%20membrane%20localization%20in%20sperm.%20Firstly%2C%20in%20mouse%2C%20we%20traced%20alpha%20V%20integrin%20gene%20expression%20during%20spermatogenesis%20in%20testicular%20fraction%20separated%20by%20elutriation%2C%20and%20we%20detected%20gene%20activity%20in%20spermatogonia%2C%20spermatocytes%2C%20and%20round%20spermatids.%20Secondly%2C%20we%20specified%20alpha%20V%20integrin%20membrane%20localization%20in%20acrosome-intact%20and%20acrosome-reacted%20sperm%20and%20compared%20its%20pattern%20between%20mouse%2C%20pig%2C%20and%20human.%20Using%20immunodetection%20and%20structured%20illumination%20microscopy%20%28SIM%29%2C%20the%20alpha%20V%20integrin%20localization%20was%20confined%20to%20the%20plasma%20membrane%20covering%20the%20acrosomal%20cap%20area%20and%20also%20to%20the%20inner%20acrosomal%20membrane%20of%20acrosome-intact%20sperm%20of%20all%20selected%20species.%20During%20the%20acrosome%20reaction%2C%20which%20was%20induced%20on%20capacitated%20sperm%2C%20the%20alpha%20V%20integrin%20relocated%20and%20was%20detected%20over%20the%20whole%20sperm%20head.%20Knowledge%20of%20the%20integrin%20pattern%20in%20mature%20sperm%20prepares%20the%20ground%20for%20further%20investigation%20into%20the%20pathologies%20and%20related%20fertility%20issues%20in%20human%20medicine%20and%20veterinary%20science.%22%2C%22date%22%3A%222021%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3390%5C%2Fijms22179525%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221422-0067%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A33Z%22%7D%7D%2C%7B%22key%22%3A%22S8S6S57C%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Stelate%20et%20al.%22%2C%22parsedDate%22%3A%222021%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BStelate%2C%20A.%3B%20Tihlarikova%2C%20E.%3B%20Schwarzerova%2C%20K.%3B%20Nedela%2C%20V.%3B%20Petrasek%2C%20J.%20%26lt%3Bb%26gt%3BCorrelative%20Light-Environmental%20Scanning%20Electron%20Microscopy%20of%20Plasma%20Membrane%20Efflux%20Carriers%20of%20Plant%20Hormone%20Auxin%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BBIOMOLECULES%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2021%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B11%26lt%3B%5C%2Fi%26gt%3B%20%2810%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fbiom11101407%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fbiom11101407%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Correlative%20Light-Environmental%20Scanning%20Electron%20Microscopy%20of%20Plasma%20Membrane%20Efflux%20Carriers%20of%20Plant%20Hormone%20Auxin%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Stelate%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Tihlarikova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Schwarzerova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Nedela%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Petrasek%22%7D%5D%2C%22abstractNote%22%3A%22Fluorescence%20light%20microscopy%20provided%20convincing%20evidence%20for%20the%20domain%20organization%20of%20plant%20plasma%20membrane%20%28PM%29%20proteins.%20Both%20peripheral%20and%20integral%20PM%20proteins%20show%20an%20inhomogeneous%20distribution%20within%20the%20PM.%20However%2C%20the%20size%20of%20PM%20nanodomains%20and%20protein%20clusters%20is%20too%20small%20to%20accurately%20determine%20their%20dimensions%20and%20nano-organization%20using%20routine%20confocal%20fluorescence%20microscopy%20and%20super-resolution%20methods.%20To%20overcome%20this%20limitation%2C%20we%20have%20developed%20a%20novel%20correlative%20light%20electron%20microscopy%20method%20%28CLEM%29%20using%20total%20internal%20reflection%20fluorescence%20microscopy%20%28TIRFM%29%20and%20advanced%20environmental%20scanning%20electron%20microscopy%20%28A-ESEM%29.%20Using%20this%20technique%2C%20we%20determined%20the%20number%20of%20auxin%20efflux%20carriers%20from%20the%20PINFORMED%20%28PIN%29%20family%20%28NtPIN3b-GFP%29%20within%20PM%20nanodomains%20of%20tobacco%20cell%20PM%20ghosts.%20Protoplasts%20were%20attached%20to%20coverslips%20and%20immunostained%20with%20anti-GFP%20primary%20antibody%20and%20secondary%20antibody%20conjugated%20to%20fluorochrome%20and%20gold%20nanoparticles.%20After%20imaging%20the%20nanodomains%20within%20the%20PM%20with%20TIRFM%2C%20the%20samples%20were%20imaged%20with%20A-ESEM%20without%20further%20processing%2C%20and%20quantification%20of%20the%20average%20number%20of%20molecules%20within%20the%20nanodomain%20was%20performed.%20Without%20requiring%20any%20post-fixation%20and%20coating%20procedures%2C%20this%20method%20allows%20to%20study%20details%20of%20the%20organization%20of%20auxin%20carriers%20and%20other%20plant%20PM%20proteins.%26lt%3B%5C%2Fp%26gt%3B%22%2C%22date%22%3A%222021%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3390%5C%2Fbiom11101407%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%222218-273X%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%2254H5F7AF%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A33Z%22%7D%7D%5D%7D
1.
Markova, V.; Hejnova, L.; Benda, A.; Novotny, J.; Melkes, B. β-Arrestin 1 and 2 Similarly Influence μ-Opioid Receptor Mobility and Distinctly Modulate Adenylyl Cyclase Activity. Cellular Signalling 2021, 87, 110124. https://doi.org/10.1016/j.cellsig.2021.110124.
1.
Hoboth, P.; Sztacho, M.; Sebesta, O.; Hozak, P. Unraveling the Phospholipid Identity of the Gene Expression Compartments by Single Molecule Localization Microscopy. Eur. Biophys. J. Biophys. Lett. 2021, 50 (SUPPL 1), 183–183.
1.
Olenici, N.; Fodor, E. The Diversity of Saproxylic Beetles’ Community from the Natural Reserve Voievodeasa Forest, North-Eastern Romania. Ann. For. Res. 2021, 64 (1), 31–60. https://doi.org/10.15287/afr.2021.2144.
1.
Stáhlavsky, F.; Kovarík, F.; Stockmann, M.; Opatova, V. Karyotype Evolution and Preliminary Molecular Assessment of Genera in the Family Scorpiopidae (Arachnida: Scorpiones). ZOOLOGY 2021, 144. https://doi.org/10.1016/j.zool.2020.125882.
1.
Hoboth, P.; Sebesta, O.; Sztacho, M.; Castano, E.; Hozák, P. Dual-Color dSTORM Imaging and ThunderSTORM Image Reconstruction and Analysis to Study the Spatial Organization of the Nuclear Phosphatidylinositol Phosphates. METHODSX 2021, 8. https://doi.org/10.1016/j.mex.2021.101372.
1.
Morgan, E. J.; Certner, M.; Lucanová, M.; Deniz, U.; Kubíková, K.; Venon, A.; Kovarík, O.; Placette, C. L.; Kolár, F. Disentangling the Components of Triploid Block and Its Fitness Consequences in Natural Diploid-Tetraploid Contact Zones of Arabidopsis Arenosa. NEW PHYTOLOGIST 2021, 232 (3), 1449–1462. https://doi.org/10.1111/nph.17357.
1.
Herrera, I. M. A.; Král, J.; Pastuchová, M.; Forman, M.; Musilová, J.; Korínková, T.; Stáhlavsky, F.; Zrzavá, M.; Nguyen, P.; Just, P.; Haddad, C. R.; Hirman, M.; Koubová, M.; Sadílek, D.; Huber, B. A. Evolutionary Pattern of Karyotypes and Meiosis in Pholcid Spiders (Araneae: Pholcidae): Implications for Reconstructing Chromosome Evolution of Araneomorph Spiders. BMC ECOLOGY AND EVOLUTION 2021, 21 (1). https://doi.org/10.1186/s12862-021-01750-8.
1.
Hoboth, P.; Sztacho, M.; Sebesta, O.; Schätz, M.; Castano, E.; Hozák, P. Nanoscale Mapping of Nuclear Phosphatidylinositol Phosphate Landscape by Dual-Color dSTORM. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 2021, 1866 (5). https://doi.org/10.1016/j.bbalip.2021.158890.
1.
Kollárová, E.; Forero, A. B.; Cvrcková, F. The Arabidopsis Thaliana Class II Formin FH13 Modulates Pollen Tube Growth. FRONTIERS IN PLANT SCIENCE 2021, 12. https://doi.org/10.3389/fpls.2021.599961.
1.
Tibenska, V.; Marvanova, A.; Elsnicova, B.; Hejnova, L.; Vebr, P.; Novotny, J.; Kolar, F.; Novakova, O.; Zurmanova, J. M. The Cardioprotective Effect Persisting during Recovery from Cold Acclimation Is Mediated by the β2-Adrenoceptor Pathway and Akt Activation. JOURNAL OF APPLIED PHYSIOLOGY 2021, 130 (3), 746–755. https://doi.org/10.1152/japplphysiol.00756.2020.
1.
Hoboth, P.; Sebesta, O.; Hozák, P. How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2021, 22 (13). https://doi.org/10.3390/ijms22136694.
1.
Palenikova, V.; Frolikova, M.; Valaskova, E.; Postlerova, P.; Komrskova, K. αV Integrin Expression and Localization in Male Germ Cells. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2021, 22 (17). https://doi.org/10.3390/ijms22179525.
1.
Stelate, A.; Tihlarikova, E.; Schwarzerova, K.; Nedela, V.; Petrasek, J. Correlative Light-Environmental Scanning Electron Microscopy of Plasma Membrane Efflux Carriers of Plant Hormone Auxin. BIOMOLECULES 2021, 11 (10). https://doi.org/10.3390/biom11101407.
2020
5891878
DKRJQ5CP
1
https://raw.githubusercontent.com/Schebique/vmcf-konfmi/refs/heads/main/vmcf-web-style.csl
50
date
desc
4983
https://web.natur.cuni.cz/sekce-bi/VMCF/wp-content/plugins/zotpress/
statussuccessupdateneededfalseinstancefalsemetarequest_last0request_next0used_cachetruedatakeyMVZ8PPGZlibraryid5891878metacreatorSummaryRotterovaetal.parsedDate2020-06-08numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtRotterovaJ.SalomakiE.PanekT.BourlandW.ZihalaD.TaborskyP.EdgcombV.P.BeinartR.A.KoliskoM.CepickaI.ltbgtGenomicsofNewCiliateLineagesProvidesInsightintotheEvolutionofObligateAnaerobiosisltbgt.ltigtCurr.Biol.ltigtltbgt2020ltbgtltigt30ltigt112037-.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.cub.2020.03.064039gthttpsdoi.org10.1016j.cub.2020.03.064ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleGenomicsofNewCiliateLineagesProvidesInsightintotheEvolutionofObligateAnaerobiosiscreatorscreatorTypeauthorfirstNameJohanalastNameRotterovacreatorTypeauthorfirstNameEriclastNameSalomakicreatorTypeauthorfirstNameTomaslastNamePanekcreatorTypeauthorfirstNameWilliamlastNameBourlandcreatorTypeauthorfirstNameDavidlastNameZihalacreatorTypeauthorfirstNamePetrlastNameTaborskycreatorTypeauthorfirstNameVirginiaP.lastNameEdgcombcreatorTypeauthorfirstNameRoxanneA.lastNameBeinartcreatorTypeauthorfirstNameMartinlastNameKoliskocreatorTypeauthorfirstNameIvanlastNameCepickaabstractNoteOxygenplaysacrucialroleinenergeticmetabolismofmosteukaryotes.Yetadaptationstolow-oxygenconcentrationsleadingtoanaerobiosishaveindependentlyariseninmanyeukaryoticlineagesresultinginabroadspectrumofreducedandmodifiedmitochondrion-relatedorganellesMROs.Inthisstudywepresentthediscoveryoftwonewclass-levellineagesoffree-livingmarineanaerobicciliatesMuranotricheacl.nov.andParablepharismeacl.nov.thattogetherwiththeclassArmophoreaformamajorcladeofobligateanaerobesAPMciliateswithintheSpirotricheaArmophoreaandLitostomateaSALgroup.TodeepenourunderstandingoftheevolutionofanaerobiosisinciliateswepredictedthemitochondrialmetabolismofculturedrepresentativesfromallthreeclassesintheAPMcladebyusingtranscriptomicandmetagenomicdataandperformedphylogenomicanalysestoassesstheirevolutionaryrelationships.ThepredictedmitochondrialmetabolismofrepresentativesfromtheAPMciliatesrevealsfunctionaladaptationsofmetabolicpathwaysthatwerepresentintheirlastcommonancestorandlikelyledtothesuccessfulcolonizationanddiversificationofthegroupinvariousanoxicenvironments.FurthermorewediscussthepossiblerelationshipofParablepharismeatotheuncultureddeep-seaclassCariacotricheaonthebasisofsingle-geneanalyses.LikemostanaerobicciliatesallstudiedspeciesoftheAPMcladehostsymbiontswhichweproposetobeasignificantacceleratingfactorinthetransitionstoanobligatelyanaerobiclifestyle.Ourresultsprovideaninsightintotheevolutionarymechanismsofearlytransitionstoanaerobiosisandshedlightonfine-scaleadaptationsinMROsoverarelativelyshortevolutionarytimeframe.dateJUN82020sectionpartNumberpartTitleDOI10.1016j.cub.2020.03.064citationKeyurlPMIDPMCIDISSN0960-98221879-0445languageEnglishcollectionsDKRJQ5CPdateModified2025-11-07T092524ZkeyHYV87KPClibraryid5891878metacreatorSummarySipkovaandRuzickaparsedDate2020-02-25numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtSipkovaH.RuzickaJ.ltbgtLarvalMorphologyofNicrophorusNicrophorusNepalensisHopeColeopteraSilphidaeNicrophorinaeltbgt.ltigtZootaxaltigtltbgt2020ltbgtltigt4743ltigt2167x2013180.ltaclass039zp-DOIURL039href039httpsdoi.org10.11646zootaxa.4743.2.2039gthttpsdoi.org10.11646zootaxa.4743.2.2ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleLarvalmorphologyofNicrophorusNicrophorusnepalensisHopeColeopteraSilphidaeNicrophorinaecreatorscreatorTypeauthorfirstNameHanalastNameSipkovacreatorTypeauthorfirstNameJanlastNameRuzickaabstractNoteLarvalmorphologyofallthreeinstarsofNicrophorusNicrophorusnepalensisHope1831ColeopteraSilphidaeNicrophorinaeisdescribedandillustratedbasedonrearedlarvae.TheeasternPalaearcticandOrientalN.N.nepalensisissimilartothewesternPalaearcticN.N.humatorGleditsch1767inanumberoflarvalcharacters.ThisiscongruentwithrecentclassificationofthegenusNicrophorusFabricius1775bySikeswhosuggestedaclosephylogeneticaffinityoftheN.nepalensisspeciesgroupwith16specieswiththeN.humatorspeciesgroup2species.ThegenericdescriptionoflarvaeofNicrophorusFabricius1775isexpandedbasedondetailedSEMobservation.dateFEB252020sectionpartNumberpartTitleDOI10.11646zootaxa.4743.2.2citationKeyurlPMIDPMCIDISSN1175-53261175-5334languageEnglishcollectionsDKRJQ5CPdateModified2025-11-07T092524ZkeySC4MT8UWlibraryid5891878metacreatorSummaryMelkesetal.parsedDate2020-01numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMelkesB.MarkovaV.HejnovaL.NovotnyJ.ltbgtx3B2-Arrestin2andERK12AreImportantMediatorsEngagedinCloseCooperationbetweenTRPV1andxB5-OpioidReceptorsinthePlasmaMembraneltbgt.ltigtInternationalJournalofMolecularSciencesltigtltbgt2020ltbgtltigt21ltigt134626.ltaclass039zp-DOIURL039href039httpsdoi.org10.3390ijms21134626039gthttpsdoi.org10.3390ijms21134626ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleu03b2-Arrestin2andERK12AreImportantMediatorsEngagedinCloseCooperationbetweenTRPV1andu00b5-OpioidReceptorsinthePlasmaMembranecreatorscreatorTypeauthorfirstNameBarboralastNameMelkescreatorTypeauthorfirstNameVendulalastNameMarkovacreatorTypeauthorfirstNameLucielastNameHejnovacreatorTypeauthorfirstNameJirilastNameNovotnyabstractNoteTheinteractionsbetweenTRPV1andu00b5-opioidreceptorsMORhaverecentlyattractedmuchattentionbecausethesetworeceptorsplayimportantrolesinpainpathwaysandcanapparentlymodulateeachotheru2019sfunctioning.Howevertheknowledgeaboutsignalinginteractionsandcrosstalkbetweenthesetworeceptorsisstilllimited.InthisstudyweinvestigatedthemutualinteractionsbetweenMORandTRPV1shortlyaftertheiractivationinHEK293cellsexpressingthesetworeceptors.Afteractivationofonereceptorweobservedsignificantchangesintheotherreceptoru2019slateralmobilityandviceversa.Howeverthechangesinreceptormovementwithintheplasmamembranewerenotconnectedwithactivationoftheotherreceptor.Wealsoobservedthatplasmamembrane-arrestin2levelswerealteredaftertreatmentwithagonistsofboththesereceptors.Knockdownof-arrestin2blockedallchangesinthelateralmobilityofbothreceptors.Furthermorewefoundthat-arrestin2canplayanimportantroleinmodulatingtheeffectivenessofERK12phosphorylationafteractivationofMORinthepresenceofTRPV1.Thesedatasuggestthat-arrestin2andERK12areimportantmediatorsbetweenthesetworeceptorsandtheirsignalingpathways.CollectivelyMORandTRPV1canmutuallyaffecteachotheru2019sbehaviorand-arrestin2apparentlyplaysakeyroleinthebidirectionalcrosstalkbetweenthesetworeceptorsintheplasmamembrane.date20201sectionpartNumberpartTitleDOI10.3390ijms21134626citationKeyurlhttpswww.mdpi.com1422-006721134626PMIDPMCIDISSN1422-0067languageencollectionsDKRJQ5CPdateModified2025-03-07T101224ZkeyZKKBCB4Ulibraryid5891878metacreatorSummarySku00e1laetal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtSkxE1laV.WalkerA.J.HorxE1kP.ltbgtSnailDefenceResponsestoParasiteInfectionTheltigtLymnaeaStagnalisltigt-ltigtTrichobilharziaSzidatiltigtModelltbgt.ltigtDEVELOPMENTALANDCOMPARATIVEIMMUNOLOGYltigtltbgt2020ltbgtltigt102ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.dci.2019.103464039gthttpsdoi.org10.1016j.dci.2019.103464ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleSnaildefenceresponsestoparasiteinfectionTheiLymnaeastagnalisi-iTrichobilharziaszidatiimodelcreatorscreatorTypeauthorfirstNameV.lastNameSku00e1lacreatorTypeauthorfirstNameA.J.lastNameWalkercreatorTypeauthorfirstNameP.lastNameHoru00e1kabstractNoteLymnaeastagnalisisacommonfreshwatergastropod.ImportantlythesnailservesastheintermediatehostformorethanonehundredspeciesofdigenetictrematodesincludingtheavianschistosomeTrichobilharziaszidatiacausativeagentofcercarialdermatitisinhumans.InfectionofL.stagnalisbyT.szidatiinitiatesadynamicconfrontationbetweenthehostandtheparasitethatculminatesinimmunocompatibilityensuringsurvivalanddevelopmentoflarvae.Unfortunatelythemolecularmechanismsdeterminingthisimmunocompatibilityremainpoorlycharacterised.ByemployingavarietyofimmuneelicitorsincludingchemicalcompoundsPAMPsandbacteriaresearchinthelasttwodecadeshaselucidatedsomeofthemolecularprocessesthatregulatethesnailinternaldefenceresponsesuchashaemocytesignallingpathways.ThesediscoveriesprovideaframeworkforfuturestudiesofmolecularinteractionsbetweenT.szidatiandL.stagnalistohelpelucidatefactorsandmechanismsenablingtransmissionofschistosomeparasites.MoreoversupportfromrecentlyavailablenextgenerationsequencedataandCRISPR-enabledfunctionalgenomicsshouldfurtherenableL.stagnalisasanimportantmodelforcomparativeimmunologyandcontributetoamorecomprehensiveunderstandingofimmunefunctionsingastropodmolluscs.date2020sectionpartNumberpartTitleDOI10.1016j.dci.2019.103464citationKeyurlPMIDPMCIDISSN0145-305XlanguagecollectionsDKRJQ5CPdateModified2025-03-19T115835ZkeyITYIDAVNlibraryid5891878metacreatorSummaryGarcu00eda-Gonzu00e1lezetal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtGarcxEDa-GonzxE1lezJ.KebrlovxE1S.SemerxE1kM.LacekJ.BabyI.K.PetrxE1sekJ.SchwarzerovxE1K.ltbgtArp23ComplexIsRequiredforAuxin-DrivenCellExpansionThroughRegulationofAuxinTransporterHomeostasisltbgt.ltigtFRONTIERSINPLANTSCIENCEltigtltbgt2020ltbgtltigt11ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.3389fpls.2020.00486039gthttpsdoi.org10.3389fpls.2020.00486ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleArp23ComplexIsRequiredforAuxin-DrivenCellExpansionThroughRegulationofAuxinTransporterHomeostasiscreatorscreatorTypeauthorfirstNameJ.lastNameGarcu00eda-Gonzu00e1lezcreatorTypeauthorfirstNameS.lastNameKebrlovu00e1creatorTypeauthorfirstNameM.lastNameSemeru00e1kcreatorTypeauthorfirstNameJ.lastNameLacekcreatorTypeauthorfirstNameI.K.lastNameBabycreatorTypeauthorfirstNameJ.lastNamePetru00e1sekcreatorTypeauthorfirstNameK.lastNameSchwarzerovu00e1abstractNoteTheArp23complexisanactinnucleatorshowntoberequiredthroughoutplantmorphogenesiscontributingtoprocessessuchascellexpansiontissuedifferentiationorcellwallassembly.ArecentpublicationdemonstratedthatplantslackingfunctionalArp23complexalsopresentdefectsinauxindistributionandtransport.ThisworkshowsthatArp23complexsubunitsarepredominantlyexpressedintheprovasculaturealthoughotherplanttissuesalsoshowpromoteractivitye.g.cotyledonsapicalmeristemsorroottip.Moreoverauxincantriggersubunitexpressionindicatingaroleofthisphytohormoneinmediatingthecomplexactivity.FurtherinvestigationofthefunctionalinteractionbetweenArp23complexandauxinsignalingalsorevealstheircooperationindeterminingpavementcellshapepresumablythroughtheroleofArp23complexinthecorrectauxincarriertrafficking.Youngseedlingsofarpc5mutantsshowincreasedauxin-triggeredproteasomaldegradationofDII-VENUSandalteredPIN3distributionwithhigherlevelsoftheproteininthevacuole.CloserobservationofvacuolarmorphologyrevealedthepresenceofamorefragmentedvacuolarcompartmentwhenArp23functionisabolishedhintingageneralizedroleofArp23complexinendomembranefunctionandproteintrafficking.date2020sectionpartNumberpartTitleDOI10.3389fpls.2020.00486citationKeyurlPMIDPMCIDISSN1664-462XlanguagecollectionsDKRJQ5CPdateModified2025-03-19T115835Zkey4VSEU77Nlibraryid5891878metacreatorSummaryHirmanetal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHirmanM.MohaganA.StxE1xE1hlavskyF.ltbgtUnexpectedlyHighNumberof18SrRNAGeneClustersinltigtMiopsalisDillyiltigtOpilionesCyphophthalmiStylocellidaefromMindanaoPhilippinesltbgt.ltigtJOURNALOFARACHNOLOGYltigtltbgt2020ltbgtltigt48ltigt3322x2013328.ltaclass039zp-DOIURL039href039httpsdoi.org10.1636JoA-S-20-028039gthttpsdoi.org10.1636JoA-S-20-028ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleUnexpectedlyhighnumberof18SrRNAgeneclustersiniMiopsalisdillyiiOpilionesCyphophthalmiStylocellidaefromMindanaoPhilippinescreatorscreatorTypeauthorfirstNameM.lastNameHirmancreatorTypeauthorfirstNameA.lastNameMohagancreatorTypeauthorfirstNameF.lastNameStu00e1u00e1hlavskyabstractNoteThesuborderCyphophthalmiArachnidaOpilionesisthesistergrouptoallremainingharvestmen.Thegrouptypicallyshowslimiteddispersalabilitiescrypticdiversityandlong-isolatedpopulations.Thesefactsmakethegroupinterestingforbiogeographicphylogeneticandcytogeneticstudies.Thesuborderisdividedintosixfamiliesallofthemwithaspecificandlongevolutionaryhistory.Howevermanyspeciesarestillundescribedandtheircytogeneticdataareonlyfragmentary.Thisfactcomplicatesthereconstructionofthemainmechanismsofkaryotypeevolutioninthisharvestmensuborderandutilizationofthecytogeneticmarkersinthetaxonomyofthismorphologicallyuniformgroupofarachnids.HerewepresentacytogeneticstudyofonespeciesofMiopsalisThorell1890ofthefamilyStylocellidaefromMindanaoPhilippines.Itskaryotypeconsistedofmainlybiarmedchromosomes2n28.Interestinglywefoundamultiplicationof18SrRNAgeneclustersinuptosevenpairswhichisoneofthehighestnumbersinknownharvestmen.TheseresultssupportthelikelypresenceofdistinctivekaryotypevariabilityinanadditionalcyphophthalmidfamilyStylocellidae2n28-30.date2020sectionpartNumberpartTitleDOI10.1636JoA-S-20-028citationKeyurlPMIDPMCIDISSN0161-8202languagecollectionsDKRJQ5CPdateModified2025-03-19T115835Zkey97UYC4D9libraryid5891878metacreatorSummaryNovaketal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtNovakJ.ZamostnaB.VopalenskyV.BuryskovaM.BurysekL.DoleckovaD.PospisekM.ltbgtInterleukin-1x3B1AssociateswiththeTumorSuppressorP53FollowingDNADamageltbgt.ltigtSCIENTIFICREPORTSltigtltbgt2020ltbgtltigt10ltigt1.ltaclass039zp-DOIURL039href039httpsdoi.org10.1038s41598-020-63779-x039gthttpsdoi.org10.1038s41598-020-63779-xltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleInterleukin-1u03b1associateswiththetumorsuppressorp53followingDNAdamagecreatorscreatorTypeauthorfirstNameJ.lastNameNovakcreatorTypeauthorfirstNameB.lastNameZamostnacreatorTypeauthorfirstNameV.lastNameVopalenskycreatorTypeauthorfirstNameM.lastNameBuryskovacreatorTypeauthorfirstNameL.lastNameBurysekcreatorTypeauthorfirstNameD.lastNameDoleckovacreatorTypeauthorfirstNameM.lastNamePospisekabstractNoteInterleukin-1alphaIL-1alphaisadual-functionproinflammatorymediator.InadditiontoitsroleinthecanonicalIL-1signalingpathwaywhichemploysmembrane-boundreceptorsagrowingbodyofevidenceshowsthatIL-1alphahassomeadditionalintracellularfunctions.WeidentifiedtheinteractionofIL-1alphawiththetumorsuppressorp53inthenucleiandcytoplasmofbothmalignantandnoncancerousmammaliancelllinesusingimmunoprecipitationandtheinsituproximityligationassayPLA.ThisinteractionwasenhancedbytreatmentwiththeantineoplasticdrugetoposidewhichsuggestsarolefortheIL-1alphacenterdotp53interactioningenotoxicstress.date2020sectionpartNumberpartTitleDOI10.1038s41598-020-63779-xcitationKeyurlPMIDPMCIDISSN2045-2322languagecollectionsDKRJQ5CPdateModified2025-03-19T115835Zkey3UJB46D3libraryid5891878metacreatorSummaryNovu00e1ketal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtNovxE1kJ.VopxE1lenskyV.PospxEDsekM.VedelerA.ltbgtCo-LocalizationofInterleukin-1x3B1andAnnexinA2atthePlasmaMembraneinResponsetoOxidativeStressltbgt.ltigtCYTOKINEltigtltbgt2020ltbgtltigt133ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.cyto.2020.155141039gthttpsdoi.org10.1016j.cyto.2020.155141ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleCo-localizationofInterleukin-1u03b1andAnnexinA2attheplasmamembraneinresponsetooxidativestresscreatorscreatorTypeauthorfirstNameJ.lastNameNovu00e1kcreatorTypeauthorfirstNameV.lastNameVopu00e1lenskycreatorTypeauthorfirstNameM.lastNamePospu00edsekcreatorTypeauthorfirstNameA.lastNameVedelerabstractNoteInterleukin-1alphaIL-1alphaandAnnexinA2AnxA2arepleiotropicmoleculeswithbothintracellularandextracellularroles.TheyshareseveralcharacteristicsincludingunconventionalsecretionaidedbyS100proteinsanchoringoftheexternalizedproteinsattheoutersurfaceoftheplasmamembraneandresponsetooxidativestress.AlthoughIL-1alphaandAnxA2havebeenimplicatedinavarietyofbiologicalprocessesincludingcancerlittleisknownaboutthemechanismsoftheircellularrelease.Inthepresentstudyemployingthenon-cancerousbreastepithelialMCF10AcellswedemonstratethatIL-1alphaandAnxA2establishacloseassociationinresponsetooxidativestress.Stressconditionsleadtotranslocationofbothproteinstowardslamellipodiarichinvimentinandassociationoffull-lengthIL-1alphaandTyr23phosphorylatedAnxA2withtheplasmamembraneatperipheralsitesdepletedofF-actin.Notablymembrane-associatedIL-1alphaandAnxA2preferentiallylocalizetotheouteredgesoftheMCF10Acellislandssuggestingthatthetwoproteinsparticipateinthecommunicationoftheseepithelialcellswiththeirneighboringcells.SimilarlyinU2OSosteosarcomacelllinebothendogenousIL-1alphaandtransientlyproducedIL-1alphaEGFPassociatewiththeplasmamembrane.WhilebenignMFC10Acellspresentmembrane-associatedIL-1alphaandAnxA2attheedgesoftheircellislandstheaggressivecancerousU2OScellscommunicateinsuchmanneralsowithdistantcells.date2020sectionpartNumberpartTitleDOI10.1016j.cyto.2020.155141citationKeyurlPMIDPMCIDISSN1043-4666languagecollectionsDKRJQ5CPdateModified2025-03-19T115836Zkey53ZCMCMNlibraryid5891878metacreatorSummaryDoubravsku00e1etal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtDoubravskxE1L.DostxE1lV.KnopF.LibusovxE1L.MacurkovxE1M.ltbgtHumanMyotubularin-RelatedProtein9RegulatesER-to-GolgiTraffickingandModulatesWNT3ASecretionltbgt.ltigtEXPERIMENTALCELLRESEARCHltigtltbgt2020ltbgtltigt386ltigt1.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.yexcr.2019.111709039gthttpsdoi.org10.1016j.yexcr.2019.111709ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleHumanmyotubularin-relatedprotein9regulatesER-to-GolgitraffickingandmodulatesWNT3AsecretioncreatorscreatorTypeauthorfirstNameL.lastNameDoubravsku00e1creatorTypeauthorfirstNameV.lastNameDostu00e1lcreatorTypeauthorfirstNameF.lastNameKnopcreatorTypeauthorfirstNameL.lastNameLibusovu00e1creatorTypeauthorfirstNameM.lastNameMacurkovu00e1abstractNoteRegulationofphosphatidylinositolphosphatesplaysacrucialroleinsignaltransductionmembranetraffickingorautophagy.Membersofthemyotubularinfamilyoflipidphosphatasescontributetophosphoinositidemetabolismbycounteractingtheactivityofphosphoinositidekinases.Themechanismsdeterminingtheirsubcellularlocalizationandtargetingtospecificmembranecompartmentsarestillpoorlyunderstood.WeshowherethattheinactivephosphataseMTMR9localizestotheintermediatecompartmentandtotheGolgiapparatusandisabletorecruititsactivephosphatasepartnersMTMR6andMTMR8totheselocations.FurthermoreMTMR8andMTMR9co-localizewiththesmallGTPaseRAB1Aandregulateitslocalization.LossofMTMR9expressioncompromisestheintegrityoftheGolgiapparatusandresultsinaltereddistributionofRAB1Aandactinnucleation-promotingfactorWHAMM.LossoroverexpressionofMTMR9leadstodecreasedrateofproteinsecretion.WedemonstratethatsecretionofphysiologicallyrelevantcargoexemplifiedbytheWNT3AproteinisaffectedafterperturbationofMTMR9levels.date2020sectionpartNumberpartTitleDOI10.1016j.yexcr.2019.111709citationKeyurlPMIDPMCIDISSN0014-4827languagecollectionsDKRJQ5CPdateModified2025-03-19T115835ZkeyD7JT887Xlibraryid5891878metacreatorSummaryJindrovu00e1etal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtJindrovxE1H.HirmanM.SadxEDlekD.BezdeckaP.StxE1hlavskyF.ltbgtDistributionof18SrDNAClustersinCentralEuropeanHarvestmenoftheSuborderEupnoiArachnidaOpilionesltbgt.ltigtEUROPEANJOURNALOFENTOMOLOGYltigtltbgt2020ltbgtltigt117ltigt282x2013288.ltaclass039zp-DOIURL039href039httpsdoi.org10.14411eje.2020.032039gthttpsdoi.org10.14411eje.2020.032ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDistributionof18SrDNAclustersinCentralEuropeanharvestmenofthesuborderEupnoiArachnidaOpilionescreatorscreatorTypeauthorfirstNameH.lastNameJindrovu00e1creatorTypeauthorfirstNameM.lastNameHirmancreatorTypeauthorfirstNameD.lastNameSadu00edlekcreatorTypeauthorfirstNameP.lastNameBezdeckacreatorTypeauthorfirstNameF.lastNameStu00e1hlavskyabstractNoterDNAclustersareanimportantcytogeneticmarkerforstudyingkaryotypeevolutionandchromosomalchanges.ThevariabilityofthiscytogeneticcharacteristicishoweverstillalmostunknowninthekaryotypesoftheentireclassArachnidaArthropodaChelicerata.ThissituationisparticularlyevidentinharvestmenArachnidaOpilioneswith97speciesstudiedcytogeneticallyforwhichthereisinformationonthenumberandpositionofrDNAclustersforonly13.MoreoverpreviousstudiesindicatethatthenumberofrDNAlociishighlyvariableinthespeciesanalysedrangingfromonetofivepairsofrDNAclusters.Basedonthisfragmentaryinformationwhichisforrarespeciesmainlyfromthelimitsofthedistributionoftheirfamiliesitisstillnotpossibletoreconstructtheancestralstateforthisimportantcytogeneticfeatureinthisorder.BuildinguponrecentresearchinCentralEuropeweanalysedthenumberandpositionof18SrDNAin13speciesbelongingtothesuborderEupnoi.Thisrevealedthattheirkaryotypeswerevariableintermsofthediploidnumber2n16-36andnumberof18SrDNAclustersfromonetosevenpairs.Forthefirsttimean18SrDNAclusterwasdetectedonBchromosomesinharvestmen.Ourstudyshedsnewlightonthekaryotypeevolutionand18SrDNAdistributioninharvestmenandprovidesanimprovedunderstandingoftheancestralstateofkaryotypesintheorderOpiliones.date2020sectionpartNumberpartTitleDOI10.14411eje.2020.032citationKeyurlPMIDPMCIDISSN1802-8829languagecollectionsDKRJQ5CPdateModified2025-03-19T115835ZkeyVDDSNU28libraryid5891878metacreatorSummaryPramaniketal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtPramanikG.KeprovaA.ValentaJ.BocanV.KvakovxE1K.LibusovaL.CiglerP.ltbgtSynthesisofNear-InfraredEmittingGoldNanoclustersforBiologicalApplicationsltbgt.ltigtJOVE-JOURNALOFVISUALIZEDEXPERIMENTSltigtltbgt2020ltbgtNo.157.ltaclass039zp-DOIURL039href039httpsdoi.org10.379160388039gthttpsdoi.org10.379160388ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleSynthesisofNear-InfraredEmittingGoldNanoclustersforBiologicalApplicationscreatorscreatorTypeauthorfirstNameG.lastNamePramanikcreatorTypeauthorfirstNameA.lastNameKeprovacreatorTypeauthorfirstNameJ.lastNameValentacreatorTypeauthorfirstNameV.lastNameBocancreatorTypeauthorfirstNameK.lastNameKvakovu00e1creatorTypeauthorfirstNameL.lastNameLibusovacreatorTypeauthorfirstNameP.lastNameCiglerabstractNoteOverthepastdecadefluorescentgoldnanoclustersAuNCshavewitnessedgrowingpopularityinbiologicalapplicationsandenormouseffortshavebeendevotedtotheirdevelopment.Inthisprotocolarecentlydevelopedfacilemethodforpreparationofwatersolublebiocompatibleandcolloidallystablenear-infraredemittingAuNCshavebeendescribedindetail.Thisroom-temperaturebottom-upchemicalsynthesisprovideseasilyfunctionalizableAuNCscappedwiththiocticacidandthiol-modifiedpolyethyleneglycolinaqueoussolution.Thesyntheticapproachrequiresneitherorganicsolventsoradditionalligandexchangenorextensiveknowledgeofsyntheticchemistrytoreproduce.TheresultingAuNCsofferfreesurfacecarboxylicacidswhichcanbefunctionalizedwithvariousbiologicalmoleculesbearingafreeaminegroupwithoutadverselyaffectingthephotoluminescentpropertiesoftheAuNCs.AquickreliableprocedureforflowcytometricquantificationandconfocalmicroscopicimagingofAuNCuptakebyHeLacellsalsobeendescribed.DuetothelargeStokesshiftpropersettingoffiltersinflowcytometryandconfocalmicroscopyisnecessaryforefficientdetectionofnear-infraredphotoluminescenceofAuNCs.date2020sectionpartNumberpartTitleDOI10.379160388citationKeyurlPMIDPMCIDISSN1940-087XlanguagecollectionsDKRJQ5CPdateModified2025-03-19T115835ZkeyAFXN7FPIlibraryid5891878metacreatorSummarySemberetal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtSemberA.PappovxE1M.FormanM.NguyenP.MarecF.DalxEDkovxE1M.DivisovxE1K.DolezxE1lkovxE1-KastxE1nkovxE1M.ZrzavxE1M.SadxEDlekD.HrubxE1B.KrxE1lJ.ltbgtPatternsofSexChromosomeDifferentiationinSpidersInsightsfromComparativeGenomicHybridisationltbgt.ltigtGENESltigtltbgt2020ltbgtltigt11ltigt8.ltaclass039zp-DOIURL039href039httpsdoi.org10.3390genes11080849039gthttpsdoi.org10.3390genes11080849ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitlePatternsofSexChromosomeDifferentiationinSpidersInsightsfromComparativeGenomicHybridisationcreatorscreatorTypeauthorfirstNameA.lastNameSembercreatorTypeauthorfirstNameM.lastNamePappovu00e1creatorTypeauthorfirstNameM.lastNameFormancreatorTypeauthorfirstNameP.lastNameNguyencreatorTypeauthorfirstNameF.lastNameMareccreatorTypeauthorfirstNameM.lastNameDalu00edkovu00e1creatorTypeauthorfirstNameK.lastNameDivisovu00e1creatorTypeauthorfirstNameM.lastNameDolezu00e1lkovu00e1-Kastu00e1nkovu00e1creatorTypeauthorfirstNameM.lastNameZrzavu00e1creatorTypeauthorfirstNameD.lastNameSadu00edlekcreatorTypeauthorfirstNameB.lastNameHrubu00e1creatorTypeauthorfirstNameJ.lastNameKru00e1labstractNoteSpidersareanintriguingmodeltoanalysesexchromosomeevolutionbecauseoftheirpeculiarmultipleXchromosomesystems.Ychromosomeswereconsideredrareinthisgrouparisingafterneo-sexchromosomeformationbyXchromosome-autosomerearrangements.HoweverrecentfindingssuggestthatYchromosomesaremorecommoninspidersthanpreviouslythought.Besidesneo-sexchromosomestheyarealsoinvolvedintheancientX1X2Ysystemofhaplogynespiderswhoseoriginisunknown.FurthermorespidersseemtoexhibitobligatorilyoneortwopairsofcryptichomomorphicXYchromosomesfurthercrypticsexchromosomepairsCSCPswhichcouldrepresenttheancestralspidersexchromosomes.HereweanalysethemoleculardifferentiationofparticulartypesofspiderYchromosomesinarepresentativesetoftenspeciesbycomparativegenomichybridisationCGH.WefoundahighYchromosomedifferentiationinhaplogynespecieswithX1X2YsystemexceptforLoxoscelesspp.CSCPchromosomesexhibitedgenerallylowdifferentiation.Possiblemechanismsandfactorsbehindtheobservedpatternsarediscussed.Thepresenceofautosomalregionsmarkedpredominantlyorexclusivelywiththemaleorfemaleprobewasalsorecorded.WeattributethispatterntointraspecificvariabilityinthecopynumberanddistributionofcertainrepetitiveDNAsinspidergenomespointingthustothelimitsofCGHinthisarachnidgroup.InadditionweconfirmednonrandomassociationofchromosomesbelongingtoparticularCSCPsatspermatogonialmitosisandspermatocytemeiosisandtheirassociationwithmultipleXsthroughoutmeiosis.TakentogetherourdatasuggestdiverseevolutionarypathwaysofmoleculardifferentiationindifferenttypesofspiderYchromosomes.date2020sectionpartNumberpartTitleDOI10.3390genes11080849citationKeyurlPMIDPMCIDISSN2073-4425languagecollectionsDKRJQ5CPdateModified2025-03-19T115835ZkeyTZCGYR3Blibraryid5891878metacreatorSummaryStu00e1hlavskyetal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtStxE1hlavskyF.FormanM.JustP.DenicF.HaddadC.R.OpatovaV.ltbgtCytogeneticsofEntelegyneSpidersArachnidaAraneaefromSouthernAfricaltbgt.ltigtCOMPARATIVECYTOGENETICSltigtltbgt2020ltbgtltigt14ltigt1107x2013138.ltaclass039zp-DOIURL039href039httpsdoi.org10.3897CompCytogen.v14i1.48667039gthttpsdoi.org10.3897CompCytogen.v14i1.48667ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleCytogeneticsofentelegynespidersArachnidaAraneaefromsouthernAfricacreatorscreatorTypeauthorfirstNameF.lastNameStu00e1hlavskycreatorTypeauthorfirstNameM.lastNameFormancreatorTypeauthorfirstNameP.lastNameJustcreatorTypeauthorfirstNameF.lastNameDeniccreatorTypeauthorfirstNameC.R.lastNameHaddadcreatorTypeauthorfirstNameV.lastNameOpatovaabstractNoteSpidersrepresentoneofthemoststudiedarachnidorders.Theyareparticularlyintriguingfromacytogeneticpointofviewduetotheircomplexanddynamicsexchromosomedeterminationsystems.DespiteintensiveresearchonthisgroupcytogeneticdatafromAfricanspidersarestillmostlylacking.Inthisstudywedescribethekaryotypesof38speciesofspidersbelongingto16entelegynefamiliesfromSouthAfricaandNamibia.Inthemajorityofanalysedfamiliestheobservedchromosomenumbersandmorphologymainlyacrocentricdidnotdeviatefromthefamily-levelcytogeneticcharacteristicsbasedonmaterialfromothercontinentsTetragnathidae2nmale24CtenidaeandOxyopidae2nmale28Sparassidae2nmale42GnaphosidaeTrachelidaeandTrochanteriidae2nmale22andSalticidae2nmale28.OntheotherhandweidentifiedinterspecificvariabilitywithinHersiliidae2nmale33and35Oecobiidae2nmale19and25Selenopidae2nmale26and29andTheridiidae2nmale21and22.WeexaminedthekaryotypesofAmmoxenidaeandGallieniellidaeforthefirsttime.Theirdiploidcounts2nmale22correspondtothesuperfamilyGnaphosoideaandsupporttheirplacementinthislineage.OntheotherhandthekaryotypesofProdidominae2nmale28and29contrastwithallotherGnaphosoidea.SimilarlytheunusuallyhighdiploidnumberinBorboropactussp.2nmale28withintheotherwisecytogeneticallyuniformfamilyThomisidaemainly2nmale21-24supportsmoleculardatasuggestingabasalpositionofthegenusinthefamily.TheimplementationofFISHmethodsforvisualisationofrDNAclustersfacilitatedthedetectionofcomplexdynamicsofnumbersoftheseloci.Weidentifieduptofivelociofthe18SrDNAclustersinoursamples.ThreedifferentsexchromosomesystemsX0X1X20andX1X2X30werealsodetectedamongthestudiedtaxa.date2020sectionpartNumberpartTitleDOI10.3897CompCytogen.v14i1.48667citationKeyurlPMIDPMCIDISSN1993-0771languagecollectionsDKRJQ5CPdateModified2025-03-19T115835ZkeyHDIWPW8Tlibraryid5891878metacreatorSummaryStu00e1hlavskyetal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtStxE1hlavskyF.NguyenP.SadxEDlekD.StundlovxE1J.JustP.HaddadC.R.KoxE7H.RanawanaK.B.StockmannM.YagmurE.A.KovarxEDkF.ltbgtEvolutionaryDynamicsofrDNAClustersonChromosomesofButhidScorpionsChelicerataArachnidaltbgt.ltigtBIOLOGICALJOURNALOFTHELINNEANSOCIETYltigtltbgt2020ltbgtltigt131ltigt3547x2013565.ltaclass039zp-DOIURL039href039httpsdoi.org10.1093biolinneanblaa118039gthttpsdoi.org10.1093biolinneanblaa118ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleEvolutionarydynamicsofrDNAclustersonchromosomesofbuthidscorpionsChelicerataArachnidacreatorscreatorTypeauthorfirstNameF.lastNameStu00e1hlavskycreatorTypeauthorfirstNameP.lastNameNguyencreatorTypeauthorfirstNameD.lastNameSadu00edlekcreatorTypeauthorfirstNameJ.lastNameStundlovu00e1creatorTypeauthorfirstNameP.lastNameJustcreatorTypeauthorfirstNameC.R.lastNameHaddadcreatorTypeauthorfirstNameH.lastNameKou00e7creatorTypeauthorfirstNameK.B.lastNameRanawanacreatorTypeauthorfirstNameM.lastNameStockmanncreatorTypeauthorfirstNameE.A.lastNameYagmurcreatorTypeauthorfirstNameF.lastNameKovaru00edkabstractNoteWeexaminedthedistributionofgenesformajorribosomalRNAsrDNAonholokineticchromosomesof74speciesbelongingto19generaofscorpionsfromthefamilyButhidaeusingfluorescenceinsituhybridizationFISH.Ouranalysisrevealeddifferencesbetweenthetwomainevolutionarylineageswithinthefamily.ThegenerabelongingtotheButhusgroup039withaproposedLaurasianoriginpossessonepairofrDNAmainlyinaninterstitialpositionwiththeonlyexceptionsbeingtheterminallocationfoundinsomeHottentottaandButhacusspeciespossiblyasaresultofchromosomefissions.Alltheremainingbuthid039groups039possessrDNAfoundstrictlyinaterminalposition.HoweverthenumberofsignalsmayincreasefromanancestralstateofonepairofrDNAlocitouptosevensignalsinReddyanusceylonensisKovariketal.2016.DespitethedifferencesinevolutionarydynamicsoftherDNAclustersbetweenthe039Buthusgroup039andotherlineagesinvestigatedwefoundahighincidenceofreciprocaltranslocationsandpresenceofmultivalentassociationsduringmeiosisinthemajorityofthegenerastudied.ThesephenomenaseemtobetypicalforthewholefamilyButhidae.date2020sectionpartNumberpartTitleDOI10.1093biolinneanblaa118citationKeyurlPMIDPMCIDISSN0024-4066languagecollectionsDKRJQ5CPdateModified2025-03-19T115835ZkeyTSUF8GHLlibraryid5891878metacreatorSummaryCifrovu00e1etal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtCifrovxE1P.OulehlovxE1D.KollxE1rovxE1E.MartinekJ.RoseroA.ZxE1rskyV.SchwarzerovxE1K.CvrckovxE1F.ltbgtDivisionofLaborBetweenTwoActinNucleators-theForminFH1andtheARP23Complex-inltigtArabidopsisltigtEpidermalCellMorphogenesisltbgt.ltigtFRONTIERSINPLANTSCIENCEltigtltbgt2020ltbgtltigt11ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.3389fpls.2020.00148039gthttpsdoi.org10.3389fpls.2020.00148ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDivisionofLaborBetweenTwoActinNucleators-theForminFH1andtheARP23Complex-iniArabidopsisiEpidermalCellMorphogenesiscreatorscreatorTypeauthorfirstNameP.lastNameCifrovu00e1creatorTypeauthorfirstNameD.lastNameOulehlovu00e1creatorTypeauthorfirstNameE.lastNameKollu00e1rovu00e1creatorTypeauthorfirstNameJ.lastNameMartinekcreatorTypeauthorfirstNameA.lastNameRoserocreatorTypeauthorfirstNameV.lastNameZu00e1rskycreatorTypeauthorfirstNameK.lastNameSchwarzerovu00e1creatorTypeauthorfirstNameF.lastNameCvrckovu00e1abstractNoteTheARP23complexandforminsaretheonlyknownplantactinnucleators.Besidestheiractin-relatedfunctionsbothsystemsalsomodulatemicrotubuleorganizationanddynamics.LossofthemainhousekeepingArabidopsisthalianaClassImembrane-targetedforminFH1At3g25500isknowntoincreasecotyledonpavementcelllobingwhilemutationsaffectingARP23subunitsexhibitanoppositeeffect.HereweexaminetheroleofFH1andtheARP23complexsubunitARPC5At4g01710inepidermalcellmorphogenesiswithfocusonpavementcellsandtrichomesusingamodelsystemofsinglefh1andarpc5aswellasdoublefh1arpc5mutants.Whilecotyledonpavementcellshapeindoublemutantsmostlyresembledsinglearpc5mutantsanalysisoftrueleafepidermalmorphologyaswellasactinandmicrotubuleorganizationanddynamicsrevealedamorecomplexrelationshipbetweenthetwosystemsandsimilarratherthanantagonisticeffectsonsomeparameters.Bothfh1andarpc5mutationsincreasedactinnetworkdensityandincreasedcellshapecomplexityinpavementcellsandtrichomesoffirsttrueleavesincontrasttocotyledons.Thuswhilethetwoactinnucleationsystemshavecomplementaryrolesinsomeaspectsofcellmorphogenesisincotyledonpavementcellstheymayactinparallelinothercelltypesanddevelopmentalstages.date2020sectionpartNumberpartTitleDOI10.3389fpls.2020.00148citationKeyurlPMIDPMCIDISSN1664-462XlanguagecollectionsDKRJQ5CPdateModified2025-03-19T115833ZkeyGP48QHZ4libraryid5891878metacreatorSummaryFrolikovaetal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtFrolikovaM.OtcenaskovaT.ValaskovxE1E.PostlerovaP.StopkovaR.StopkaP.KomrskovaK.ltbgtTheRoleofTasteReceptormTAS1R3inChemicalCommunicationofGametesltbgt.ltigtINTERNATIONALJOURNALOFMOLECULARSCIENCESltigtltbgt2020ltbgtltigt21ltigt7.ltaclass039zp-DOIURL039href039httpsdoi.org10.3390ijms21072651039gthttpsdoi.org10.3390ijms21072651ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTheRoleofTasteReceptormTAS1R3inChemicalCommunicationofGametescreatorscreatorTypeauthorfirstNameM.lastNameFrolikovacreatorTypeauthorfirstNameT.lastNameOtcenaskovacreatorTypeauthorfirstNameE.lastNameValaskovu00e1creatorTypeauthorfirstNameP.lastNamePostlerovacreatorTypeauthorfirstNameR.lastNameStopkovacreatorTypeauthorfirstNameP.lastNameStopkacreatorTypeauthorfirstNameK.lastNameKomrskovaabstractNoteFertilizationisamultiplestepprocessleadingtothefusionoffemaleandmalegametesandtheformationofazygote.Besidesdirectgametemembraneinteractionviabindingreceptorslocalizedonbothoocyteandspermsurfacefertilizationalsoinvolvesgametecommunicationviachemicalmoleculestriggeringvarioussignalingpathways.ThisworkfocusesonamousetastereceptormTAS1R3encodedbytheTas1r3geneasapotentialreceptormediatingchemicalcommunicationbetweengametesusingtheC57BL6Jlabmousestrain.InordertospecifytheroleofmTAS1R3weaimedtocharacterizeitspreciselocalizationintestisandspermusingsuperresolutionmicroscopy.Thetestiscryo-sectionacrosome-intactspermreleasedfromcaudaepididymisandspermwhichunderwenttheacrosomereactionARwereevaluated.ThemTAS1R3receptorwasdetectedinlatespermatidswheretheacrosomewasbeingformedandintheacrosomalcapofacrosomeintactsperm.ARistriggeredinmiceduringspermmaturationinthefemalereproductivetractandbypassingthroughtheeggsurroundingssuchascumulusoophoruscells.ThisARonsetisindependentoftheextracellularmatrixoftheoocytecalledzonapellucida.AfterARtherelocationofmTAS1R3totheequatorialsegmentwasobservedandthereceptorremainedexposedtotheoutersurroundingsofthefemalereproductivetractwhereitsphysiologicalligandtheaminoacidL-glutamatenaturallyoccurs.ThereforewetargetedthepossibleinteractioninvitrobetweenthemTAS1R3andL-glutamateasapartofchemicalcommunicationbetweenspermandeggandusedananti-mTAS1R3-specificantibodytoblockit.WedetectedthattheacrosomereactedspermatozoashowedachemotacticresponseinthepresenceofL-glutamateduringandaftertheARanditislikelythatmTAS1R3actedasitsmediator.date2020sectionpartNumberpartTitleDOI10.3390ijms21072651citationKeyurlPMIDPMCIDISSN1422-0067languagecollectionsDKRJQ5CPdateModified2025-03-19T115833Zkey65FX8KCSlibraryid5891878metacreatorSummaryNajdrovu00e1etal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtNajdrovxE1V.StairsC.W.VinopalovxE1M.VolemanL.DolezalP.ltbgtTheEvolutionofthePufSuperfamilyofProteinsacrosstheTreeofEukaryotesltbgt.ltigtBMCBIOLOGYltigtltbgt2020ltbgtltigt18ltigt1.ltaclass039zp-DOIURL039href039httpsdoi.org10.1186s12915-020-00814-3039gthttpsdoi.org10.1186s12915-020-00814-3ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTheevolutionofthePufsuperfamilyofproteinsacrossthetreeofeukaryotescreatorscreatorTypeauthorfirstNameV.lastNameNajdrovu00e1creatorTypeauthorfirstNameC.W.lastNameStairscreatorTypeauthorfirstNameM.lastNameVinopalovu00e1creatorTypeauthorfirstNameL.lastNameVolemancreatorTypeauthorfirstNameP.lastNameDolezalabstractNoteBackgroundEukaryoticgeneexpressioniscontrolledbyanumberofRNA-bindingproteinsRBPsuchastheproteinsfromthePufPumilioandFBFsuperfamilyPufSF.TheseproteinsbindtoRNAviamultiplePufrepeatdomainseachofwhichspecificallyrecognizesasingleRNAbase.RecentlythreediversifiedPufSFproteinshavebeendescribedinmodelorganismseachofwhichisresponsibleforthematurationofribosomalRNAorthetranslationalregulationofmRNAshoweverlessisknownabouttheroleoftheseproteinsacrosseukaryoticdiversity.ResultsHereweinvestigatedthedistributionandfunctionofPufSFRBPsinthetreeofeukaryotes.WedeterminedthatthefollowingPufSFproteinsareuniversallyconservedacrosseukaryotesandcanbebroadlyclassifiedintothreegroupsiNop9orthologueswhichparticipateinthenucleolarprocessingofimmature18SrRNAii039classical039PufswhichcontrolthetranslationofmRNAandiiiPUM3orthologueswhichareinvolvedinthematurationof7SrRNA.InnearlyalleukaryotestherRNAmaturationproteinsNop9andPUM3areretainedasasinglecopywhilemRNAeffectors039classical039Pufsunderwentmultiplelineage-specificexpansions.Weproposethatthevariationinnumberof039classical039PufsrelatestothesizeofthetranscriptomeandthusthepotentialmRNAtargets.WefurtherdistinguishedfullsetofPufSFproteinsindivergentmetamonadGiardiaintestinalisandinitiatedtheircellularandbiochemicalcharacterization.ConclusionsOurdatasuggestthatthelasteukaryoticcommonancestorLECAalreadycontainedallthreetypesofPufSFproteinsandthat039classical039Pufsthenunderwentlineage-specificexpansions.date2020sectionpartNumberpartTitleDOI10.1186s12915-020-00814-3citationKeyurlPMIDPMCIDISSN1741-7007languagecollectionsDKRJQ5CPdateModified2025-03-19T115833ZkeyR7Z2H7BZlibraryid5891878metacreatorSummaryTibenskaetal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtTibenskaV.BenesovaA.VebrP.LiptakovaA.HejnovxE1L.ElsnicovxE1B.DrahotaZ.HornikovaD.GalatxEDkF.KolarD.VybiralS.AlxE1novxE1P.NovotnyJ.KolarF.NovakovaO.ZurmanovaJ.M.ltbgtGradualColdAcclimationInducesCardioprotectionwithoutAffectingAdrenergicx3B2-Receptor-MediatedAdenylylCyclaseSignalingltbgt.ltigtJOURNALOFAPPLIEDPHYSIOLOGYltigtltbgt2020ltbgtltigt128ltigt41023x20131032.ltaclass039zp-DOIURL039href039httpsdoi.org10.1152japplphysiol.00511.2019039gthttpsdoi.org10.1152japplphysiol.00511.2019ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleGradualcoldacclimationinducescardioprotectionwithoutaffectingadrenergicu03b2-receptor-mediatedadenylylcyclasesignalingcreatorscreatorTypeauthorfirstNameV.lastNameTibenskacreatorTypeauthorfirstNameA.lastNameBenesovacreatorTypeauthorfirstNameP.lastNameVebrcreatorTypeauthorfirstNameA.lastNameLiptakovacreatorTypeauthorfirstNameL.lastNameHejnovu00e1creatorTypeauthorfirstNameB.lastNameElsnicovu00e1creatorTypeauthorfirstNameZ.lastNameDrahotacreatorTypeauthorfirstNameD.lastNameHornikovacreatorTypeauthorfirstNameF.lastNameGalatu00edkcreatorTypeauthorfirstNameD.lastNameKolarcreatorTypeauthorfirstNameS.lastNameVybiralcreatorTypeauthorfirstNameP.lastNameAlu00e1novu00e1creatorTypeauthorfirstNameJ.lastNameNovotnycreatorTypeauthorfirstNameF.lastNameKolarcreatorTypeauthorfirstNameO.lastNameNovakovacreatorTypeauthorfirstNameJ.M.lastNameZurmanovaabstractNoteNovelstrategiesareneededthatcanstimulateendogenoussignalingpathwaystoprotecttheheartfrommyocardialinfarction.ThepresentstudytestedthehypothesisthatappropriateregimenofcoldacclimationCAmayprovideapromisingapproachforimprovingmyocardialresistancetoischemiareperfusionURinjurywithoutnegativesideeffects.WeevaluatedmyocardialURinjurymitochondrialswellingandbeta-adrenergicreceptorbeta-AR-adenylylcyclase-mediatedsignaling.MaleWistarratswereexposedtoCA8degreesC8hdayforaweekfollowedby4wkat8degreesCfor24hdaywhiletherecoverygroupCARwaskeptat24degreesCforanadditional2wk.Themyocardialinfarctioninducedbycoronaryocclusionfor20minfollowedby3-hreperfusionwasreducedfrom56incontrolsto30and23afterCAandCAR.respectively.Inlinetherateofmitochondrialswellingat200muMCa2wasdecreasedinbothgroups.AcuteadministrationofmetoprololdecreasedinfarctionincontrolgroupanddidnotaffecttheCA-elicitedcardiprotection.Accordinglyneitherbeta1-AR-Gsalpha-adenyly-1-cyclasesignaling.stimulatedwithspecificligandsnorp-PKAPICAratioswereaffectedafterCAorCAR.Importantly.Westernblotandimmunofluorescenceanalysesrevealedbeta2-andbeta3-ARproteinenrichmentinmembranesinbothexperimentalgroups.WeconcludethatgradualcoldacclimationresultsinapersistingincreaseofmyocardialresistancetoIRinjurywithouthypertensionandhypertrophy.ThecardioprotectivephenotypeisassociatedwithunalteredadenylylcyclasesignalingandincreasedmitochondrialresistancetoCa2-overload.Thepotentialroleofupregulatedbeta2beta3-ARpathwaysremainstobeelucidated.NEWampNOTEWORTHYWepresentanewmodelofmildgradualcoldacclimationincreasingtolerancetomyocardialischemiareperfusioninjurywithouthypertensionandhypertrophy.CardioprotectivephenotypeisaccompaniedbyunalteredadenylylcyclasesignalingandincreasedmitochondrialresistancetoCa2-overload.Thepotentialroleofupregulatedbeta2beta3-adrenoreceptoractivationisconsidered.Thesefindingsmaystimulatethedevelopmentofnovelpreventiveandtherapeuticstrategiesagainstmyocardialischemiareperfusioninjury.date2020sectionpartNumberpartTitleDOI10.1152japplphysiol.00511.2019citationKeyurlPMIDPMCIDISSN8750-7587languagecollectionsDKRJQ5CPdateModified2025-03-19T115833ZkeyIZIZB2KClibraryid5891878metacreatorSummaryMarkovicetal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMarkovicV.CvrckovxE1F.PotockyM.KulichI.PejcharP.KollxE1rovxE1E.SynekL.ZxE1rskyV.ltbgtEXO70A2IsCriticalforExocystComplexFunctioninPollenDevelopmentltbgt.ltigtPLANTPHYSIOLOGYltigtltbgt2020ltbgtltigt184ltigt41823x20131839.ltaclass039zp-DOIURL039href039httpsdoi.org10.1104pp.19.01340039gthttpsdoi.org10.1104pp.19.01340ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleEXO70A2IsCriticalforExocystComplexFunctioninPollenDevelopmentcreatorscreatorTypeauthorfirstNameV.lastNameMarkoviccreatorTypeauthorfirstNameF.lastNameCvrckovu00e1creatorTypeauthorfirstNameM.lastNamePotockycreatorTypeauthorfirstNameI.lastNameKulichcreatorTypeauthorfirstNameP.lastNamePejcharcreatorTypeauthorfirstNameE.lastNameKollu00e1rovu00e1creatorTypeauthorfirstNameL.lastNameSynekcreatorTypeauthorfirstNameV.lastNameZu00e1rskyabstractNoteApollen-specificcomponentoftheexocystaproteincomplexregulatingcellularsecretionplaysanimportantroleinpollendevelopmentandfunctioninArabidopsis.Pollendevelopmentpollengraingerminationandpollentubeelongationarecrucialbiologicalprocessesinangiospermplantsthatneedpreciseregulationtodeliverspermcellstoovulesforfertilization.Highlypolarizedsecretionatagrowingpollentubetiprequirestheexocysttetheringcomplexresponsibleforspecifictargetingofsecretoryvesiclestotheplasmamembrane.HerewedemonstratethatArabidopsisArabidopsisthalianaEXO70A2At5g52340isthemainexocystEXO70isoforminthemalegametophytegoverningtheconventionalsecretoryfunctionoftheexocystanalogoustoEXO70A1At5g03540inthesporophyte.OuranalysisofaCRISPR-generatedexo70a2mutantrevealedthatEXO70A2isessentialforefficientpollenmaturationpollengraingerminationandpollentubegrowth.GFPEXO70A2waslocalizedtothenucleusandcytoplasmindevelopingpollengrainsandlatertotheapicaldomainingrowingpollentubetipscharacterizedbyintensiveexocytosis.MoreoverEXO70A2couldsubstituteforEXO70A1functioninthesporophytebutnotviceversaindicatingpartialfunctionalredundancyofthesetwocloselyrelatedisoformsandhigherspecificityofEXO70A2forpollendevelopment-relatedprocesses.PhylogeneticanalysisrevealedthattheancientduplicationofEXO70Aoneofwhichisalwayshighlyexpressedinpollenoccurredindependentlyinmonocotsanddicots.InsummaryEXO70A2isacrucialcomponentoftheexocystcomplexinArabidopsispollenthatisrequiredforefficientplantsexualreproduction.date2020sectionpartNumberpartTitleDOI10.1104pp.19.01340citationKeyurlPMIDPMCIDISSN0032-0889languagecollectionsDKRJQ5CPdateModified2025-03-19T115833ZkeyRLAXHA3Rlibraryid5891878metacreatorSummaryJankovicovaetal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtJankovicovaJ.FrolikovaM.PalenikovaV.ValaskovaE.CernyJ.SecovaP.BartokovaM.HorovskaL.Manaskova-PostlerovaP.AntalikovaJ.KomrskovaK.ltbgtExpressionandDistributionofCD151asaPartnerofAlpha6IntegrininMaleGermCellsltbgt.ltigtSCIENTIFICREPORTSltigtltbgt2020ltbgtltigt10ltigt1.ltaclass039zp-DOIURL039href039httpsdoi.org10.1038s41598-020-61334-2039gthttpsdoi.org10.1038s41598-020-61334-2ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleExpressionanddistributionofCD151asapartnerofalpha6integrininmalegermcellscreatorscreatorTypeauthorfirstNameJ.lastNameJankovicovacreatorTypeauthorfirstNameM.lastNameFrolikovacreatorTypeauthorfirstNameV.lastNamePalenikovacreatorTypeauthorfirstNameE.lastNameValaskovacreatorTypeauthorfirstNameJ.lastNameCernycreatorTypeauthorfirstNameP.lastNameSecovacreatorTypeauthorfirstNameM.lastNameBartokovacreatorTypeauthorfirstNameL.lastNameHorovskacreatorTypeauthorfirstNameP.lastNameManaskova-PostlerovacreatorTypeauthorfirstNameJ.lastNameAntalikovacreatorTypeauthorfirstNameK.lastNameKomrskovaabstractNoteThephysiologicalimportanceofCD151tetraspaninisknownfromsomaticcellsanditsoutside-insignallingthroughintegrinswasdescribed.InmalegermcellstwotetraspaninsCD9andCD81areinvolvedinsperm-eggmembranefusionandsimilarlytointegrinstheyoccupycharacteristicregions.WereporthereonanewlydiscoveredpresenceofCD151inspermandpresentitsexpressionanddistributionduringspermatogenesisandspermtransitionduringtheacrosomereaction.WetracedCD151geneandproteinexpressionintesticularcellsubpopulationswithstrongenrichmentinspermatogoniaandspermatids.ThetesticularandepididymallocalizationpatternisdesignatedtothespermheadprimaryfusionsitecalledtheequatorialsegmentandwhencomparedtotheacrosomevesiclestatusCD151waslocatedintotheinneracrosomalmembraneoverlyingthenucleus.MoreoverweshowCD151interactionwithalpha6integrinsubunitwhichformsadimerwithbeta4asapartofcisproteininteractionswithinspermpriortogametefusion.Weusedmammalianspecieswithdistinctspermmorphologyandspermmaturationsuchasmouseandbullandcomparedtheresultswithhuman.InconclusionthedeliveredfindingscharacteriseCD151asanovelspermtetraspaninnetworkmemberandprovideknowledgeonitsphysiologyinmalegermcells.date2020sectionpartNumberpartTitleDOI10.1038s41598-020-61334-2citationKeyurlPMIDPMCIDISSN2045-2322languagecollectionsDKRJQ5CPdateModified2025-03-19T115833Zkey8LCTTR9Alibraryid5891878metacreatorSummaryBarcyteetal.parsedDate2020numChildren3bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtBarcyteD.PilatovaJ.MojzesP.NedbalovaL.ltbgtTheArcticltigtCylindrocystisltigtZygnematophyceaeStreptophytaGreenAlgaeAreGeneticallyandMorphologicallyDiverseandExhibitEffectiveAccumulationofPolyphosphateltbgt.ltigtJOURNALOFPHYCOLOGYltigtltbgt2020ltbgtltigt56ltigt1217x2013232.ltaclass039zp-DOIURL039href039httpsdoi.org10.1111jpy.12931039gthttpsdoi.org10.1111jpy.12931ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTheArcticiCylindrocystisiZygnematophyceaeStreptophytaGreenAlgaeareGeneticallyandMorphologicallyDiverseandExhibitEffectiveAccumulationofPolyphosphatecreatorscreatorTypeauthorfirstNameD.lastNameBarcytecreatorTypeauthorfirstNameJ.lastNamePilatovacreatorTypeauthorfirstNameP.lastNameMojzescreatorTypeauthorfirstNameL.lastNameNedbalovaabstractNoteThegreenalgalgenusCylindrocystisiswidespreadinvarioustypesofenvironmentsincludingextremehabitats.Howeververylittleisknownaboutitsdiversityespeciallyinpolarregions.InthepresentstudyweisolatedsevennewCylindrocystis-likestrainsfromterrestrialandfreshwaterhabitatsinSvalbardHighArctic.WeaimedtocomparethenewisolatesonamolecularrbcLand18SrDNAmorphologicallightandconfocallaserscanningmicroscopyandcytologicalRamanmicroscopybasis.OurresultsdemonstratedthattheArcticCylindrocystiswerenotofamonophyleticoriginandthatthestudiedstrainsclusteredwithintwocladestentativelynamedthesoilandfreshwaterglaciercladesandfourseparatelineages.Morphologicaldatacellsizeshapeandchloroplastmorphologysupportedthepresenceofseveraldistincttaxaamongthenewisolates.MoreovertheresultsshowedthattheArcticCylindrocystisstrainswerecloselyrelatedtostrainsoriginatingfromthetemperatezoneindicatinghighecologicalversatilityandsuccessfullong-distancedispersalofthegenus.LargeamountsofinorganicpolyphosphatepolyPgrainsweredetectedwithinthechloroplastsoftheculturedArcticCylindrocystisstrainssuggestingeffectiveluxuryuptakeofphosphorus.AdditionallyvariousintracellularstructureswereidentifiedusingRamanmicroscopyandcytochemicalandfluorescentstaining.ThisstudyrepresentsthefirstattempttocombinemolecularmorphologicalecologicalandbiogeographicaldataforArcticCylindrocystis.OurnovelcytologicalobservationspartiallyexplainthesuccessofCylindrocystis-likemicroalgaeinpolarregions.date2020sectionpartNumberpartTitleDOI10.1111jpy.12931citationKeyurlPMIDPMCIDISSN0022-3646languagecollectionsDKRJQ5CPdateModified2025-03-07T114047ZkeyJT3PH8UJlibraryid5891878metacreatorSummaryMelkesetal.parsedDate2020numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMelkesB.MarkovaV.HejnovaL.MarekA.NovotnyJ.ltbgtNaloxoneIsaPotentialBindingLigandandActivatoroftheCapsaicinReceptorTRPV1ltbgt.ltigtBiologicalandPharmaceuticalBulletinltigtltbgt2020ltbgtltigt43ltigt5908x2013912.ltaclass039zp-DOIURL039href039httpsdoi.org10.1248bpb.b19-00806039gthttpsdoi.org10.1248bpb.b19-00806ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleNaloxoneIsaPotentialBindingLigandandActivatoroftheCapsaicinReceptorTRPV1creatorscreatorTypeauthorfirstNameBarboralastNameMelkescreatorTypeauthorfirstNameVendulalastNameMarkovacreatorTypeauthorfirstNameLucielastNameHejnovacreatorTypeauthorfirstNameAleslastNameMarekcreatorTypeauthorfirstNameJirilastNameNovotnyabstractNoteThereceptorchanneltransientreceptorpotentialvanilloid1TRPV1functionsasasensorofnoxiousheatandvariouschemicals.ThereisincreasingevidenceforacrosstalkbetweenTRPV1andopioidreceptors.HereweinvestigatedtheeffectoftheprototypicalTRPV1agonistcapsaicinandselectedopioidligandsonTRPV1movementintheplasmamembraneandintracellularcalciumlevelsinHEK293cellsexpressingTRPV1taggedwithcyanfluorescentproteinCFP.WeobservedthatlateralmobilityofTRPV1increasedaftertreatmentofcellswithcapsaicinornaloxoneanonselectiveopioidreceptorantagonistbutnotwithDAMGOau03bc-opioidreceptoragonist.InterestinglybothcapsaicinandnaloxoneunlikeDAMGOelicitedintracellularcalciumresponses.TheincreasedTRPV1movementandcalciuminfluxinducedbycapsaicinandnaloxonewereblockedbytheTRPV1antagonistcapsazepine.TheabilityofnaloxonetodirectlyinteractwithTRPV1wasfurthercorroboratedby3H-naloxonebinding.InconclusionourdatasuggestthatbesidesactingasanopioidreceptorantagonistnaloxonemayfunctionasapotentialTRPV1agonist.nntnntGraphicalAbstractntFullsizeImagedate2020sectionpartNumberpartTitleDOI10.1248bpb.b19-00806citationKeyurlPMIDPMCIDISSNlanguagecollectionsDKRJQ5CPdateModified2025-03-07T101302Z
1.
Rotterova, J.; Salomaki, E.; Panek, T.; Bourland, W.; Zihala, D.; Taborsky, P.; Edgcomb, V. P.; Beinart, R. A.; Kolisko, M.; Cepicka, I. Genomics of New Ciliate Lineages Provides Insight into the Evolution of Obligate Anaerobiosis. Curr. Biol. 2020, 30 (11), 2037-+. https://doi.org/10.1016/j.cub.2020.03.064.
1.
Sipkova, H.; Ruzicka, J. Larval Morphology of Nicrophorus (Nicrophorus) Nepalensis Hope (Coleoptera: Silphidae: Nicrophorinae). Zootaxa 2020, 4743 (2), 167–180. https://doi.org/10.11646/zootaxa.4743.2.2.
1.
Melkes, B.; Markova, V.; Hejnova, L.; Novotny, J. β-Arrestin 2 and ERK1/2 Are Important Mediators Engaged in Close Cooperation between TRPV1 and µ-Opioid Receptors in the Plasma Membrane. International Journal of Molecular Sciences 2020, 21 (13), 4626. https://doi.org/10.3390/ijms21134626.
1.
Skála, V.; Walker, A. J.; Horák, P. Snail Defence Responses to Parasite Infection: The Lymnaea Stagnalis-Trichobilharzia Szidati Model. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 2020, 102. https://doi.org/10.1016/j.dci.2019.103464.
1.
García-González, J.; Kebrlová, S.; Semerák, M.; Lacek, J.; Baby, I. K.; Petrásek, J.; Schwarzerová, K. Arp2/3 Complex Is Required for Auxin-Driven Cell Expansion Through Regulation of Auxin Transporter Homeostasis. FRONTIERS IN PLANT SCIENCE 2020, 11. https://doi.org/10.3389/fpls.2020.00486.
1.
Hirman, M.; Mohagan, A.; Stááhlavsky, F. Unexpectedly High Number of 18S rRNA Gene Clusters in Miopsalis Dillyi (Opiliones: Cyphophthalmi: Stylocellidae) from Mindanao, Philippines. JOURNAL OF ARACHNOLOGY 2020, 48 (3), 322–328. https://doi.org/10.1636/JoA-S-20-028.
1.
Novak, J.; Zamostna, B.; Vopalensky, V.; Buryskova, M.; Burysek, L.; Doleckova, D.; Pospisek, M. Interleukin-1α Associates with the Tumor Suppressor P53 Following DNA Damage. SCIENTIFIC REPORTS 2020, 10 (1). https://doi.org/10.1038/s41598-020-63779-x.
1.
Novák, J.; Vopálensky, V.; Pospísek, M.; Vedeler, A. Co-Localization of Interleukin-1α and Annexin A2 at the Plasma Membrane in Response to Oxidative Stress. CYTOKINE 2020, 133. https://doi.org/10.1016/j.cyto.2020.155141.
1.
Doubravská, L.; Dostál, V.; Knop, F.; Libusová, L.; Macurková, M. Human Myotubularin-Related Protein 9 Regulates ER-to-Golgi Trafficking and Modulates WNT3A Secretion. EXPERIMENTAL CELL RESEARCH 2020, 386 (1). https://doi.org/10.1016/j.yexcr.2019.111709.
1.
Jindrová, H.; Hirman, M.; Sadílek, D.; Bezdecka, P.; Stáhlavsky, F. Distribution of 18S rDNA Clusters in Central European Harvestmen of the Suborder Eupnoi (Arachnida: Opiliones). EUROPEAN JOURNAL OF ENTOMOLOGY 2020, 117, 282–288. https://doi.org/10.14411/eje.2020.032.
1.
Pramanik, G.; Keprova, A.; Valenta, J.; Bocan, V.; Kvaková, K.; Libusova, L.; Cigler, P. Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS 2020, No. 157. https://doi.org/10.3791/60388.
1.
Sember, A.; Pappová, M.; Forman, M.; Nguyen, P.; Marec, F.; Dalíková, M.; Divisová, K.; Dolezálková-Kastánková, M.; Zrzavá, M.; Sadílek, D.; Hrubá, B.; Král, J. Patterns of Sex Chromosome Differentiation in Spiders: Insights from Comparative Genomic Hybridisation. GENES 2020, 11 (8). https://doi.org/10.3390/genes11080849.
1.
Stáhlavsky, F.; Forman, M.; Just, P.; Denic, F.; Haddad, C. R.; Opatova, V. Cytogenetics of Entelegyne Spiders (Arachnida, Araneae) from Southern Africa. COMPARATIVE CYTOGENETICS 2020, 14 (1), 107–138. https://doi.org/10.3897/CompCytogen.v14i1.48667.
1.
Stáhlavsky, F.; Nguyen, P.; Sadílek, D.; Stundlová, J.; Just, P.; Haddad, C. R.; Koç, H.; Ranawana, K. B.; Stockmann, M.; Yagmur, E. A.; Kovarík, F. Evolutionary Dynamics of rDNA Clusters on Chromosomes of Buthid Scorpions (Chelicerata: Arachnida). BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY 2020, 131 (3), 547–565. https://doi.org/10.1093/biolinnean/blaa118.
1.
Cifrová, P.; Oulehlová, D.; Kollárová, E.; Martinek, J.; Rosero, A.; Zársky, V.; Schwarzerová, K.; Cvrcková, F. Division of Labor Between Two Actin Nucleators-the Formin FH1 and the ARP2/3 Complex-in Arabidopsis Epidermal Cell Morphogenesis. FRONTIERS IN PLANT SCIENCE 2020, 11. https://doi.org/10.3389/fpls.2020.00148.
1.
Frolikova, M.; Otcenaskova, T.; Valasková, E.; Postlerova, P.; Stopkova, R.; Stopka, P.; Komrskova, K. The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2020, 21 (7). https://doi.org/10.3390/ijms21072651.
1.
Najdrová, V.; Stairs, C. W.; Vinopalová, M.; Voleman, L.; Dolezal, P. The Evolution of the Puf Superfamily of Proteins across the Tree of Eukaryotes. BMC BIOLOGY 2020, 18 (1). https://doi.org/10.1186/s12915-020-00814-3.
1.
Tibenska, V.; Benesova, A.; Vebr, P.; Liptakova, A.; Hejnová, L.; Elsnicová, B.; Drahota, Z.; Hornikova, D.; Galatík, F.; Kolar, D.; Vybiral, S.; Alánová, P.; Novotny, J.; Kolar, F.; Novakova, O.; Zurmanova, J. M. Gradual Cold Acclimation Induces Cardioprotection without Affecting Adrenergic β-Receptor-Mediated Adenylyl Cyclase Signaling. JOURNAL OF APPLIED PHYSIOLOGY 2020, 128 (4), 1023–1032. https://doi.org/10.1152/japplphysiol.00511.2019.
1.
Markovic, V.; Cvrcková, F.; Potocky, M.; Kulich, I.; Pejchar, P.; Kollárová, E.; Synek, L.; Zársky, V. EXO70A2 Is Critical for Exocyst Complex Function in Pollen Development. PLANT PHYSIOLOGY 2020, 184 (4), 1823–1839. https://doi.org/10.1104/pp.19.01340.
1.
Jankovicova, J.; Frolikova, M.; Palenikova, V.; Valaskova, E.; Cerny, J.; Secova, P.; Bartokova, M.; Horovska, L.; Manaskova-Postlerova, P.; Antalikova, J.; Komrskova, K. Expression and Distribution of CD151 as a Partner of Alpha6 Integrin in Male Germ Cells. SCIENTIFIC REPORTS 2020, 10 (1). https://doi.org/10.1038/s41598-020-61334-2.
1.
Barcyte, D.; Pilatova, J.; Mojzes, P.; Nedbalova, L. The Arctic Cylindrocystis (Zygnematophyceae, Streptophyta) Green Algae Are Genetically and Morphologically Diverse and Exhibit Effective Accumulation of Polyphosphate. JOURNAL OF PHYCOLOGY 2020, 56 (1), 217–232. https://doi.org/10.1111/jpy.12931.
1.
Melkes, B.; Markova, V.; Hejnova, L.; Marek, A.; Novotny, J. Naloxone Is a Potential Binding Ligand and Activator of the Capsaicin Receptor TRPV1. Biological and Pharmaceutical Bulletin 2020, 43 (5), 908–912. https://doi.org/10.1248/bpb.b19-00806.
2019
5891878
Z57M64S6
1
https://raw.githubusercontent.com/Schebique/vmcf-konfmi/refs/heads/main/vmcf-web-style.csl
50
date
desc
4983
https://web.natur.cuni.cz/sekce-bi/VMCF/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%228F8SXT57%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Remias%20et%20al.%22%2C%22parsedDate%22%3A%222020-02%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BRemias%2C%20D.%3B%20Prochazkova%2C%20L.%3B%20Nedbalova%2C%20L.%3B%20Andersen%2C%20R.%20A.%20%26lt%3Bb%26gt%3BTwo%20New%20Kremastochrysopsis%20Species%2C%20K.%20Austriaca%20Sp.%20Nov.%20and%20K.%20Americana%20Sp.%20Nov.%20%28Chrysophyceae%291%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BJ.%20Phycol.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2020%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B56%26lt%3B%5C%2Fi%26gt%3B%20%281%29%2C%20135%26%23x2013%3B145.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fjpy.12937%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fjpy.12937%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Two%20New%20Kremastochrysopsis%20species%2C%20K.%20austriaca%20sp.%20nov.%20and%20K.%20americana%20sp.%20nov.%20%28Chrysophyceae%291%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%22%2C%22lastName%22%3A%22Remias%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lenka%22%2C%22lastName%22%3A%22Prochazkova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Linda%22%2C%22lastName%22%3A%22Nedbalova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%20A.%22%2C%22lastName%22%3A%22Andersen%22%7D%5D%2C%22abstractNote%22%3A%22Melting%20summer%20snow%20in%20the%20Austrian%20Alps%20exhibited%20a%20yellowish%20bloom%20that%20was%20mainly%20comprised%20of%20an%20unidentified%20unicellular%20chrysophyte.%20Molecular%20data%20%2818S%20rRNA%20and%20rbcL%20genes%29%20showed%20a%20close%20relationship%20to%20published%20sequences%20from%20an%20American%20pond%20alga%20formerly%20identified%20as%20Kremastochrysis%20sp.%20The%20genera%20Kremastochrysis%20and%20Kremastochrysopsis%20are%20morphologically%20distinguished%20by%20the%20number%20of%20flagella%20observed%20with%20the%20light%20microscope%2C%20and%20therefore%20we%20assigned%20the%20Austrian%20snow%20alga%20and%20an%20American%20pond%20alga%20to%20the%20genus%20Kremastochrysopsis.%20Transmission%20and%20scanning%20electron%20microscopy%20revealed%20that%20swimming%20cells%20had%20two%20flagella%20oriented%20in%20opposite%20directions%2C%20typical%20for%20the%20Hibberdiales.%20Molecular%20phylogenetic%20analyses%20showed%20that%20both%20new%20species%20were%20closely%20related%20to%20Hibberdia.%20Kremastochrysopsis%20ocellata%2C%20the%20type%20species%20and%20only%20known%20species%2C%20has%20two%20chloroplasts%20per%20cell%20and%20the%20zoospores%20have%20red%20eyespots.%20Our%20two%20organisms%20had%20only%20a%20single%20chloroplast%20and%20no%20zoospore%20eyespot%2C%20but%20their%20gene%20sequences%20differed%20substantially.%20Therefore%2C%20we%20described%20two%20new%20species%2C%20Kremastochrysopsis%20austriaca%20sp.%20nov%20and%20Kremstochrysopsis%20americana%20sp.%20nov.%20When%20grown%20in%20culture%2C%20both%20taxa%20showed%20a%20characteristic%20hyponeustonic%20growth%20%28hanging%20below%20the%20water%20surface%29%2C%20whereas%20older%20immotile%20cells%20grew%20at%20the%20bottom%20of%20the%20culture%20vessel.%20Ecologically%2C%20Kremastochrysopsis%20austriaca%20sp.%20nov.%2C%20which%20caused%20snow%20discolorations%2C%20had%20no%20close%20phylogenetic%20relationships%20to%20other%20psychrophilic%20chrysophytes%2C%20for%20example%2C%20Chromulina%20chionophilia%2C%20Hydrurus%20sp.%2C%20and%20Ochromonas-like%20flagellates.%22%2C%22date%22%3A%22FEB%202020%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2Fjpy.12937%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220022-3646%2C%201529-8817%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A26%3A44Z%22%7D%7D%2C%7B%22key%22%3A%22HI4NP2UI%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Kaderka%20et%20al.%22%2C%22parsedDate%22%3A%222019-11-11%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BKaderka%2C%20R.%3B%20Bulantov%26%23xE1%3B%2C%20J.%3B%20Heneberg%2C%20P.%3B%20%26%23x158%3Bez%26%23xE1%3B%26%23x10D%3B%2C%20M.%20%26lt%3Bb%26gt%3BUrticating%20Setae%20of%20Tarantulas%20%28Araneae%3A%20Theraphosidae%29%3A%20Morphology%2C%20Revision%20of%20Typology%20and%20Terminology%20and%20Implications%20for%20Taxonomy%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BPLOS%20ONE%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B14%26lt%3B%5C%2Fi%26gt%3B%20%2811%29%2C%20e0224384.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0224384%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0224384%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Urticating%20setae%20of%20tarantulas%20%28Araneae%3A%20Theraphosidae%29%3A%20Morphology%2C%20revision%20of%20typology%20and%20terminology%20and%20implications%20for%20taxonomy%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Radan%22%2C%22lastName%22%3A%22Kaderka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jana%22%2C%22lastName%22%3A%22Bulantov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petr%22%2C%22lastName%22%3A%22Heneberg%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Milan%22%2C%22lastName%22%3A%22%5Cu0158ez%5Cu00e1%5Cu010d%22%7D%5D%2C%22abstractNote%22%3A%22Tarantula%20urticating%20setae%20are%20modified%20setae%20located%20on%20the%20abdomen%20or%20pedipalps%2C%20which%20represent%20an%20effective%20defensive%20mechanism%20against%20vertebrate%20or%20invertebrate%20predators%20and%20intruders.%20They%20are%20also%20useful%20taxonomic%20tools%20as%20morphological%20characters%20facilitating%20the%20classification%20of%20New%20World%20theraphosid%20spiders.%20In%20the%20present%20study%2C%20the%20morphology%20of%20urticating%20setae%20was%20studied%20on%20144%20taxa%20of%20New%20World%20theraphosids%2C%20including%20ontogenetic%20stages%20in%20chosen%20species%2C%20except%20for%20species%20with%20urticating%20setae%20of%20type%20VII.%20The%20typology%20of%20urticating%20setae%20was%20revised%2C%20and%20types%20I%2C%20III%20and%20IV%20were%20redescribed.%20The%20urticating%20setae%20in%20spiders%20with%20type%20I%20setae%2C%20which%20were%20originally%20among%20type%20III%20or%20were%20considered%20setae%20of%20intermediate%20morphology%20between%20types%20I%20and%20III%2C%20are%20newly%20considered%20to%20be%20ontogenetic%20derivatives%20of%20type%20I%20and%20are%20described%20as%20subtypes.%20Setae%20of%20intermediate%20morphology%20between%20that%20of%20body%20setae%20and%20type%20II%20urticating%20setae%20that%20were%20found%20in%20Iridopelma%20hirsutum%20and%20Antillena%20rickwesti%20may%20provide%20another%20evidence%20that%20type%20II%20urticating%20setae%20evolved%20from%20body%20setae.%20It%20is%20supposed%20that%20the%20fusion%20of%20barbs%20with%20the%20shaft%20may%20lead%20to%20the%20morphology%20of%20type%20II%20setae.%20As%20the%20type%20II%20setae%20of%20Aviculariinae%20evolved%20independently%20to%20the%20UrS%20of%20Theraphosinae%20and%20both%20subfamilies%20represent%20two%20non-sister%20groups%2C%20this%20should%20explain%20the%20differences%20in%20the%20morphology%20of%20body%20setae%20in%20Aviculariinae%20and%20Theraphosinae.%20The%20terminology%20of%20%5Cu201cbarbs%5Cu201d%20and%20%5Cu201creversed%20barbs%5Cu201d%20was%20revised%20and%20redefined%2C%20newly%20emphasizing%20the%20real%20direction%20of%20barbs.%22%2C%22date%22%3A%22Nov%2011%2C%202019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.pone.0224384%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.plos.org%5C%2Fplosone%5C%2Farticle%3Fid%3D10.1371%5C%2Fjournal.pone.0224384%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221932-6203%22%2C%22language%22%3A%22en%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A34Z%22%7D%7D%2C%7B%22key%22%3A%22VS24TDUY%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Oulehlov%5Cu00e1%20et%20al.%22%2C%22parsedDate%22%3A%222019-08-01%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BOulehlov%26%23xE1%3B%2C%20D.%3B%20Koll%26%23xE1%3Brov%26%23xE1%3B%2C%20E.%3B%20Cifrov%26%23xE1%3B%2C%20P.%3B%20Pejchar%2C%20P.%3B%20%26%23x17D%3B%26%23xE1%3Brsk%26%23xFD%3B%2C%20V.%3B%20Cvr%26%23x10D%3Bkov%26%23xE1%3B%2C%20F.%20%26lt%3Bb%26gt%3BArabidopsis%20Class%20I%20Formin%20FH1%20Relocates%20between%20Membrane%20Compartments%20during%20Root%20Cell%20Ontogeny%20and%20Associates%20with%20Plasmodesmata%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BPlant%20Cell%20Physiol%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B60%26lt%3B%5C%2Fi%26gt%3B%20%288%29%2C%201855%26%23x2013%3B1870.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-ItemURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fpcp%5C%2Fpcz102%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fpcp%5C%2Fpcz102%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Arabidopsis%20Class%20I%20Formin%20FH1%20Relocates%20between%20Membrane%20Compartments%20during%20Root%20Cell%20Ontogeny%20and%20Associates%20with%20Plasmodesmata%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Denisa%22%2C%22lastName%22%3A%22Oulehlov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eva%22%2C%22lastName%22%3A%22Koll%5Cu00e1rov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petra%22%2C%22lastName%22%3A%22Cifrov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P%5Cu0159emysl%22%2C%22lastName%22%3A%22Pejchar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Viktor%22%2C%22lastName%22%3A%22%5Cu017d%5Cu00e1rsk%5Cu00fd%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fatima%22%2C%22lastName%22%3A%22Cvr%5Cu010dkov%5Cu00e1%22%7D%5D%2C%22abstractNote%22%3A%22Formins%20are%20evolutionarily%20conserved%20eukaryotic%20proteins%20engaged%20in%20actin%20nucleation%20and%20other%20aspects%20of%20cytoskeletal%20organization.%20Angiosperms%20have%20two%20formin%20clades%20with%20multiple%20paralogs%3B%20typical%20plant%20Class%20I%20formins%20are%20integral%20membrane%20proteins%20that%20can%20anchor%20cytoskeletal%20structures%20to%20membranes.%20For%20the%20main%20Arabidopsis%20housekeeping%20Class%20I%20formin%2C%20FH1%20%28At3g25500%29%2C%20plasmalemma%20localization%20was%20documented%20in%20heterologous%20expression%20and%20overexpression%20studies.%20We%20previously%20showed%20that%20loss%20of%20FH1%20function%20increases%20cotyledon%20epidermal%20pavement%20cell%20shape%20complexity%20via%20modification%20of%20actin%20and%20microtubule%20organization%20and%20dynamics.%20Here%2C%20we%20employ%20transgenic%20Arabidopsis%20expressing%20green%20fluorescent%20protein-tagged%20FH1%20%28FH1-GFP%29%20from%20its%20native%20promoter%20to%20investigate%20in%20vivo%20behavior%20of%20this%20formin%20using%20advanced%20microscopy%20techniques.%20The%20fusion%20protein%20is%20functional%2C%20since%20its%20expression%20complements%20the%20fh1%20loss-of-function%20mutant%20phenotype.%20Accidental%20overexpression%20of%20FH1-GFP%20results%20in%20a%20decrease%20in%20trichome%20branch%20number%2C%20while%20fh1%20mutation%20has%20the%20opposite%20effect%2C%20indicating%20a%20general%20role%20of%20this%20formin%20in%20controlling%20cell%20shape%20complexity.%20Consistent%20with%20previous%20reports%2C%20FH1-GFP%20associates%20with%20membranes.%20However%2C%20the%20protein%20exhibits%20surprising%20actin-%20and%20secretory%20pathway-dependent%20dynamic%20localization%20and%20relocates%20between%20cellular%20endomembranes%20and%20the%20plasmalemma%20during%20cell%20division%20and%20differentiation%20in%20root%20tissues%2C%20with%20transient%20tonoplast%20localization%20at%20the%20transition%5C%2Felongation%20zones%20border.%20FH1-GFP%20also%20accumulates%20in%20actin-rich%20regions%20of%20cortical%20cytoplasm%20and%20associates%20with%20plasmodesmata%20in%20both%20the%20cotyledon%20epidermis%20and%20root%20tissues.%20Together%20with%20previous%20reports%20from%20metazoan%20systems%2C%20this%20suggests%20that%20formins%20might%20have%20a%20shared%20%28ancestral%20or%20convergent%29%20role%20at%20cell%5Cu2013cell%20junctions.%22%2C%22date%22%3A%222019-08-01%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fpcp%5C%2Fpcz102%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fpcp%5C%2Fpcz102%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220032-0781%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-09-06T09%3A09%3A47Z%22%7D%7D%2C%7B%22key%22%3A%22R423DAU6%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Lukes%20and%20Hylis%22%2C%22parsedDate%22%3A%222019-06%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BLukes%2C%20J.%3B%20Hylis%2C%20M.%20%26lt%3Bb%26gt%3BIn%20Love%20with%20Microsporidia%20for%2060%2BYears%3A%20Jiri%20Vavra%20Passed%20Away%20IN%20MEMORIAM%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BJ.%20Eukaryot.%20Microbiol.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B66%26lt%3B%5C%2Fi%26gt%3B%20%283%29%2C%20533%26%23x2013%3B534.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fjeu.12721%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fjeu.12721%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22In%20Love%20with%20Microsporidia%20for%2060%2BYears%3A%20Jiri%20Vavra%20Passed%20Away%20IN%20MEMORIAM%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julius%22%2C%22lastName%22%3A%22Lukes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Miroslav%22%2C%22lastName%22%3A%22Hylis%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%22MAY-JUN%202019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2Fjeu.12721%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221066-5234%2C%201550-7408%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A26%3A45Z%22%7D%7D%2C%7B%22key%22%3A%22QZQ8KA78%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Nemcova%20and%20Kapustin%22%2C%22parsedDate%22%3A%222019-05%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BNemcova%2C%20Y.%3B%20Kapustin%2C%20D.%20%26lt%3Bb%26gt%3BMallomonas%20Teres%2C%20Sp.%20Nov.%20%28Chrysophyceae%29%2C%20Simultaneously%20Revealed%20in%20Two%20Distant%20European%20Peat-Bog%20Regions%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BCryptogam.%20Algol.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B40%26lt%3B%5C%2Fi%26gt%3B%20%284%29%2C%2035%26%23x2013%3B39.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.5252%5C%2Fcryptogamie-algologe2019v40a4%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.5252%5C%2Fcryptogamie-algologe2019v40a4%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Mallomonas%20teres%2C%20sp.%20nov.%20%28Chrysophyceae%29%2C%20simultaneously%20revealed%20in%20two%20distant%20European%20peat-bog%20regions%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yvonne%22%2C%22lastName%22%3A%22Nemcova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dmitry%22%2C%22lastName%22%3A%22Kapustin%22%7D%5D%2C%22abstractNote%22%3A%22We%20present%20the%20description%20of%20Mallomonas%20teres%2C%20sp.%20nov.%2C%20from%20three%20localities%2C%20two%20peat-bogs%20in%20Northern%20Sweden%20and%20a%20peat-bog%20pool%20in%20the%20Polessian%20Nature%20Reserve%2C%20Northern%20Ukraine.%20The%20sites%20ranged%20from%20acidic%20to%20neutral%20pH%2C%20were%20low%20in%20conductivity%2C%20and%20contained%20other%20pH-indifferent%20or%20low-pH%20chrysophyte%20taxa.%20According%20to%20the%20scale%20structure%20M.%20teres%2C%20sp.%20nov.%2C%20belongs%20to%20the%20section%20Heterospinae%20within%20the%20genus.%20The%20most%20distinctive%20scale%20character%20discerning%20M.%20teres%2C%20sp.%20nov.%2C%20from%20the%20other%20species%20of%20the%20section%20Heterospinae%20is%20the%20distal%20part%20of%20the%20scale%2C%20which%20has%20a%20smooth%20dome%20and%20numerous%20parallel%20curved%20ribs.%20Mallomonas%20teres%2C%20sp.%20nov.%2C%20is%20probably%20distributed%20at%20least%20in%20temperate%2C%20acidic%20European%20localities.%20Its%20rare%20occurrence%20could%20be%20a%20result%20of%20a%20poor%20dispersal%20capacity%20combined%20with%20a%20low%20growth%20rate.%22%2C%22date%22%3A%22MAY%202019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.5252%5C%2Fcryptogamie-algologe2019v40a4%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220181-1568%2C%201776-0984%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A26%3A45Z%22%7D%7D%2C%7B%22key%22%3A%22H53B2NJE%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Palma-Onetto%20et%20al.%22%2C%22parsedDate%22%3A%222019-03%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BPalma-Onetto%2C%20V.%3B%20Pflegerova%2C%20J.%3B%20Plarre%2C%20R.%3B%20Synek%2C%20J.%3B%20Cvacka%2C%20J.%3B%20Sillam-Dusses%2C%20D.%3B%20Sobotnik%2C%20J.%20%26lt%3Bb%26gt%3BThe%20Labral%20Gland%20in%20Termites%3A%20Evolution%20and%20Function%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BBiol.%20J.%20Linnean%20Soc.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B126%26lt%3B%5C%2Fi%26gt%3B%20%283%29%2C%20587%26%23x2013%3B597.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fbiolinnean%5C%2Fbly212%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fbiolinnean%5C%2Fbly212%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20labral%20gland%20in%20termites%3A%20evolution%20and%20function%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Valeria%22%2C%22lastName%22%3A%22Palma-Onetto%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jitka%22%2C%22lastName%22%3A%22Pflegerova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rudy%22%2C%22lastName%22%3A%22Plarre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jiri%22%2C%22lastName%22%3A%22Synek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Josef%22%2C%22lastName%22%3A%22Cvacka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Sillam-Dusses%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jan%22%2C%22lastName%22%3A%22Sobotnik%22%7D%5D%2C%22abstractNote%22%3A%22Termites%20are%20important%20contributors%20to%20ecosystem%20functioning.%20They%20are%20highly%20abundant%20in%20tropical%20and%20sub-tropical%20habitats%2C%20and%20represent%20an%20important%20resource%20for%20a%20wide%20range%20of%20predators.%20Their%20evolutionary%20success%20is%20driven%20largely%20by%20a%20life%20in%20populous%20colonies%20with%20a%20complex%20communication%20system%20controlled%20by%20a%20rich%20set%20of%20exocrine%20glands%20whose%20secretions%20are%20involved%20in%20many%20aspects%20of%20termite%20life.%20As%20many%20as%2020%20different%20exocrine%20organs%20are%20known%20to%20occur%20in%20termites.%20Among%20them%2C%20the%20labral%20gland%20has%20been%20largely%20understudied.%20Here%20we%20examine%20the%20structure%20of%20the%20labral%20gland%20in%20workers%20of%2028%20species%20and%20imagoes%20of%2033%20species%20across%20all%20termite%20taxa%2C%20and%20in%20the%20Cryptocercus%20wood%20roach.%20The%20labral%20gland%20is%20present%20in%20all%20species%2C%20and%20comprises%20two%20secretory%20regions%20located%20on%20the%20ventral%20side%20of%20the%20labrum%20and%20the%20dorso-apical%20part%20of%20the%20hypopharynx%2C%20respectively.%20The%20epithelium%20of%20the%20gland%20consists%20of%20class%201%20secretory%20cells%20with%20an%20abundance%20of%20smooth%20endoplasmic%20reticulum%2C%20and%20long%20microvilli%20with%20a%20channel%20inside%2C%20which%20releases%20secretion%20through%20a%20modified%20cuticle.%20Our%20observations%20suggest%20that%20the%20labral%20gland%20is%20involved%20in%20defensive%20communication%20after%20encounter%20with%20a%20non-nestmate.%22%2C%22date%22%3A%22MAR%202019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fbiolinnean%5C%2Fbly212%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220024-4066%2C%201095-8312%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A26%3A45Z%22%7D%7D%2C%7B%22key%22%3A%22PIGGPYUG%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Leontovy%5Cu010d%20et%20al.%22%2C%22parsedDate%22%3A%222019-02-04%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BLeontovy%26%23x10D%3B%2C%20R.%3B%20Young%2C%20N.%20D.%3B%20Korhonen%2C%20P.%20K.%3B%20Hall%2C%20R.%20S.%3B%20Bulantov%26%23xE1%3B%2C%20J.%3B%20Je%26%23x159%3B%26%23xE1%3Bbkov%26%23xE1%3B%2C%20V.%3B%20Ka%26%23x161%3Bn%26%23xFD%3B%2C%20M.%3B%20Gasser%2C%20R.%20B.%3B%20Hor%26%23xE1%3Bk%2C%20P.%20%26lt%3Bb%26gt%3BMolecular%20Evidence%20for%20Distinct%20Modes%20of%20Nutrient%20Acquisition%20between%20Visceral%20and%20Neurotropic%20Schistosomes%20of%20Birds%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BSci%20Rep%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B9%26lt%3B%5C%2Fi%26gt%3B%20%281%29%2C%201347.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-018-37669-2%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-018-37669-2%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Molecular%20evidence%20for%20distinct%20modes%20of%20nutrient%20acquisition%20between%20visceral%20and%20neurotropic%20schistosomes%20of%20birds%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Roman%22%2C%22lastName%22%3A%22Leontovy%5Cu010d%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Neil%20D.%22%2C%22lastName%22%3A%22Young%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pasi%20K.%22%2C%22lastName%22%3A%22Korhonen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ross%20S.%22%2C%22lastName%22%3A%22Hall%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jana%22%2C%22lastName%22%3A%22Bulantov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Veronika%22%2C%22lastName%22%3A%22Je%5Cu0159%5Cu00e1bkov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22Ka%5Cu0161n%5Cu00fd%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robin%20B.%22%2C%22lastName%22%3A%22Gasser%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petr%22%2C%22lastName%22%3A%22Hor%5Cu00e1k%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Trichobilharzia%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20species%20are%20parasitic%20flatworms%20%28called%20schistosomes%20or%20flukes%29%20that%20cause%20important%20diseases%20in%20birds%20and%20humans%2C%20but%20very%20little%20is%20known%20about%20their%20molecular%20biology.%20Here%2C%20using%20a%20transcriptomics-bioinformatics-based%20approach%2C%20we%20explored%20molecular%20aspects%20pertaining%20to%20the%20nutritional%20requirements%20of%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Trichobilharzia%20szidati%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%28%5Cu2018visceral%20fluke%5Cu2019%29%20and%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20T%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20regenti%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%28%5Cu2018neurotropic%20fluke%5Cu2019%29%20in%20their%20avian%20host.%20We%20studied%20the%20larvae%20of%20each%20species%20before%20they%20enter%20%28cercariae%29%20and%20as%20they%20migrate%20%28schistosomules%29%20through%20distinct%20tissues%20in%20their%20avian%20%28duck%29%20host.%20Cercariae%20of%20both%20species%20were%20enriched%20for%20pathways%20or%20molecules%20associated%20predominantly%20with%20carbohydrate%20metabolism%2C%20oxidative%20phosphorylation%20and%20translation%20of%20proteins%20linked%20to%20ribosome%20biogenesis%2C%20exosome%20production%20and%5C%2For%20lipid%20biogenesis.%20Schistosomules%20of%20both%20species%20were%20enriched%20for%20pathways%20or%20molecules%20associated%20with%20processes%20including%20signal%20transduction%2C%20cell%20turnover%20and%20motility%2C%20DNA%20replication%20and%20repair%2C%20molecular%20transport%20and%5C%2For%20catabolism.%20Comparative%20informatic%20analyses%20identified%20molecular%20repertoires%20%28within%2C%20e.g.%2C%20peptidases%20and%20secretory%20proteins%29%20in%20schistosomules%20that%20can%20broadly%20degrade%20macromolecules%20in%20both%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20T.%20szidati%20and%20T.%20regenti%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%2C%20and%20others%20that%20are%20tailored%20to%20each%20species%20to%20selectively%20acquire%20nutrients%20from%20particular%20tissues%20through%20which%20it%20migrates.%20Thus%2C%20this%20study%20provides%20molecular%20evidence%20for%20distinct%20modes%20of%20nutrient%20acquisition%20between%20the%20visceral%20and%20neurotropic%20flukes%20of%20birds.%22%2C%22date%22%3A%222019-02-04%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-018-37669-2%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-018-37669-2%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%222045-2322%22%2C%22language%22%3A%22en%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-10T08%3A14%3A42Z%22%7D%7D%2C%7B%22key%22%3A%22XNKJIWI3%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Musilova%20et%20al.%22%2C%22parsedDate%22%3A%222019%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BMusilova%2C%20Z.%3B%20Indermaur%2C%20A.%3B%20Bitja-Nyom%2C%20A.%20R.%3B%20Omelchenko%2C%20D.%3B%20K%26%23x142%3Bodawska%2C%20M.%3B%20Albergati%2C%20L.%3B%20Remi%26%23x161%3Bov%26%23xE1%3B%2C%20K.%3B%20Salzburger%2C%20W.%20%26lt%3Bb%26gt%3BEvolution%20of%20the%20Visual%20Sensory%20System%20in%20Cichlid%20Fishes%20from%20Crater%20Lake%20Barombi%20Mbo%20in%20Cameroon%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BMolecular%20Ecology%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B28%26lt%3B%5C%2Fi%26gt%3B%20%2823%29%2C%205010%26%23x2013%3B5031.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fmec.15217%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fmec.15217%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Evolution%20of%20the%20visual%20sensory%20system%20in%20cichlid%20fishes%20from%20crater%20lake%20Barombi%20Mbo%20in%20Cameroon%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zuzana%22%2C%22lastName%22%3A%22Musilova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adrian%22%2C%22lastName%22%3A%22Indermaur%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Arnold%20Roger%22%2C%22lastName%22%3A%22Bitja-Nyom%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dmytro%22%2C%22lastName%22%3A%22Omelchenko%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Monika%22%2C%22lastName%22%3A%22K%5Cu0142odawska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lia%22%2C%22lastName%22%3A%22Albergati%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kate%5Cu0159ina%22%2C%22lastName%22%3A%22Remi%5Cu0161ov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Walter%22%2C%22lastName%22%3A%22Salzburger%22%7D%5D%2C%22abstractNote%22%3A%22In%20deep-water%20animals%2C%20the%20visual%20sensory%20system%20is%20often%20challenged%20by%20the%20dim-light%20environment.%20Here%2C%20we%20focus%20on%20the%20molecular%20mechanisms%20involved%20in%20rapid%20deep-water%20adaptations.%20We%20examined%20visual%20system%20evolution%20in%20a%20small-scale%20yet%20phenotypically%20and%20ecologically%20diverse%20adaptive%20radiation%2C%20the%20species%20flock%20of%20cichlid%20fishes%20in%20deep%20crater%20lake%20Barombi%20Mbo%20in%20Cameroon%2C%20West%20Africa.%20We%20show%20that%20rapid%20adaptations%20of%20the%20visual%20system%20to%20the%20novel%20deep-water%20habitat%20primarily%20occurred%20at%20the%20level%20of%20gene%20expression%20changes%20rather%20than%20through%20nucleotide%20mutations%2C%20which%20is%20compatible%20with%20the%20young%20age%20of%20the%20radiation.%20Based%20on%20retinal%20bulk%20RNA%20sequencing%20of%20all%20eleven%20species%2C%20we%20found%20that%20the%20opsin%20gene%20expression%20pattern%20was%20substantially%20different%20for%20the%20deep-water%20species.%20The%20nine%20shallow-water%20species%20feature%20an%20opsin%20palette%20dominated%20by%20the%20red-sensitive%20%28LWS%29%20opsin%2C%20whereas%20the%20two%20unrelated%20deep-water%20species%20lack%20expression%20of%20LWS%20and%20the%20violet-sensitive%20%28SWS2B%29%20opsin%2C%20thereby%20shifting%20the%20cone%20sensitivity%20to%20the%20centre%20of%20the%20light%20spectrum.%20Deep-water%20species%20further%20predominantly%20express%20the%20green-sensitive%20RH2A%5Cu03b1%20over%20RH2A%5Cu03b2.%20We%20identified%20one%20amino%20acid%20substitution%20in%20the%20RH2A%5Cu03b1%20opsin%20specific%20to%20the%20deep-water%20species.%20We%20finally%20performed%20a%20comparative%20gene%20expression%20analysis%20in%20retinal%20tissue%20of%20deep-%20vs.%20shallow-water%20species.%20We%20thus%20identified%2046%20differentially%20expressed%20genes%2C%20many%20of%20which%20are%20associated%20with%20functions%20in%20vision%2C%20hypoxia%20management%20or%20circadian%20clock%20regulation%2C%20with%20some%20of%20them%20being%20associated%20with%20human%20eye%20diseases.%22%2C%22date%22%3A%222019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2Fmec.15217%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fonlinelibrary.wiley.com%5C%2Fdoi%5C%2Fabs%5C%2F10.1111%5C%2Fmec.15217%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221365-294X%22%2C%22language%22%3A%22en%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-09-06T09%3A06%3A59Z%22%7D%7D%2C%7B%22key%22%3A%223RWYHDF8%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Kohutova%20et%20al.%22%2C%22parsedDate%22%3A%222019%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BKohutova%2C%20J.%3B%20Elsnicova%2C%20B.%3B%20Holzerova%2C%20K.%3B%20Neckar%2C%20J.%3B%20Sebesta%2C%20O.%3B%20Jezkova%2C%20J.%3B%20Vecka%2C%20M.%3B%20Vebr%2C%20P.%3B%20Hornikova%2C%20D.%3B%20Bacova%2C%20B.%20S.%3B%20Benova%2C%20T.%20E.%3B%20Hlavackova%2C%20M.%3B%20Tribulova%2C%20N.%3B%20Kolar%2C%20F.%3B%20Novakoval%2C%20O.%3B%20Zurmanova%2C%20J.%20M.%20%26lt%3Bb%26gt%3BAnti-Arrhythmic%20Cardiac%20Phenotype%20Elicited%20by%20Chronic%20Intermittent%20Hypoxia%20Is%20Associated%20With%20Alterations%20in%20Connexin-43%20Expression%2C%20Phosphorylation%2C%20and%20Distribution%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BFRONTIERS%20IN%20ENDOCRINOLOGY%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B9%26lt%3B%5C%2Fi%26gt%3B.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffendo.2018.00789%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffendo.2018.00789%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Anti-arrhythmic%20Cardiac%20Phenotype%20Elicited%20by%20Chronic%20Intermittent%20Hypoxia%20Is%20Associated%20With%20Alterations%20in%20Connexin-43%20Expression%2C%20Phosphorylation%2C%20and%20Distribution%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Kohutova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%22%2C%22lastName%22%3A%22Elsnicova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Holzerova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Neckar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Sebesta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Jezkova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Vecka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Vebr%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Hornikova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20S.%22%2C%22lastName%22%3A%22Bacova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%20E.%22%2C%22lastName%22%3A%22Benova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Hlavackova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22N.%22%2C%22lastName%22%3A%22Tribulova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22Kolar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Novakoval%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20M.%22%2C%22lastName%22%3A%22Zurmanova%22%7D%5D%2C%22abstractNote%22%3A%22Remodeling%20of%20the%20cellular%20distribution%20of%20gap%20junctions%20formed%20mainly%20by%20connexin-43%20%28Cx43%29%20can%20be%20related%20to%20the%20increased%20incidence%20of%20cardiac%20arrhythmias.%20It%20has%20been%20shown%20that%20adaptation%20to%20chronic%20intermittent%20hypobaric%20hypoxia%20%28IHH%29%20attenuates%20the%20incidence%20and%20severity%20of%20ischemic%20and%20reperfusion%20ventricular%20arrhythmias%20and%20increases%20the%20proportion%20of%20anti-arrhythmic%20n-3%20polyunsaturated%20fatty%20acids%20%28n-3%20PUFA%29%20in%20heart%20phospholipids.%20Wistar%20rats%20were%20exposed%20to%20simulated%20IHH%20%287%2C000%20m%2C%208-h%5C%2Fday%2C%2035%20exposures%29%20and%20compared%20with%20normoxic%20controls%20%28N%29.%20Cx43%20expression%2C%20phosphorylation%2C%20localization%20and%20n-3%20PUFA%20proportion%20were%20analyzed%20in%20left%20ventricular%20myocardium.%20Compared%20to%20N%2C%20IHH%20led%20to%20higher%20expression%20of%20total%20Cx43%2C%20its%20variant%20phosphorylated%20at%20Ser368%20%5Bp-Cx43%28Ser368%29%5D%2C%20which%20maintains%20%26quot%3Bend%20to%20end%26quot%3B%20communication%2C%20as%20well%20as%20p-Cx43%28Ser364%5C%2F365%29%2C%20which%20facilitates%20conductivity.%20By%20contrast%2C%20expression%20of%20non-phosphorylated%20Cx43%20and%20p-Cx43%28Ser278%5C%2F289%29%2C%20attenuating%20intercellular%20communication%2C%20was%20lower%20in%20IHH%20than%20in%20N.%20IHH%20also%20resulted%20in%20increased%20expression%20of%20protein%20kinase%20A%20and%20protein%20kinase%20G%20while%20casein%20kinase%201%20did%20not%20change%20compared%20to%20N.%20In%20IHH%20group%2C%20which%20exhibited%20reduced%20incidence%20of%20ischemic%20ventricular%20arrhythmias%2C%20Cx43%20and%20p-Cx43%28Ser368%29%20were%20more%20abundant%20at%20%26quot%3Bend%20to%20end%26quot%3B%20gap%20junctions%20than%20in%20N%20group%20and%20this%20difference%20was%20preserved%20after%20acute%20regional%20ischemia%20%2810%20min%29.%20We%20further%20confirmed%20higher%20n-3%20PUFA%20proportion%20in%20heart%20phospholipids%20after%20adaptation%20to%20IHH%2C%20which%20was%20even%20further%20increased%20by%20ischemia.%20Our%20results%20suggest%20that%20adaptation%20to%20IHH%20alters%20expression%2C%20phosphorylation%20and%20distribution%20of%20Cx43%20as%20well%20as%20cardioprotective%20n-3PUFA%20proportion%20suggesting%20that%20the%20anti-arrhythmic%20phenotype%20elicited%20by%20IHH%20can%20be%20at%20least%20partly%20related%20to%20the%20stabilization%20of%20the%20%26quot%3Bend%20to%20end%26quot%3B%20conductivity%20between%20cardiomyocytes%20during%20brief%20ischemia.%22%2C%22date%22%3A%222019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3389%5C%2Ffendo.2018.00789%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221664-2392%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A36Z%22%7D%7D%2C%7B%22key%22%3A%22FI4BPEMB%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Kub%5Cu00e1tov%5Cu00e1%20et%20al.%22%2C%22parsedDate%22%3A%222019%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BKub%26%23xE1%3Btov%26%23xE1%3B%2C%20Z.%3B%20Pejchar%2C%20P.%3B%20Potocky%2C%20M.%3B%20Sekeres%2C%20J.%3B%20Z%26%23xE1%3Brsky%2C%20V.%3B%20Kulich%2C%20I.%20%26lt%3Bb%26gt%3BArabidopsis%20Trichome%20Contains%20Two%20Plasma%20Membrane%20Domains%20with%20Different%20Lipid%20Compositions%20Which%20Attract%20Distinct%20EXO70%20Subunits%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BINTERNATIONAL%20JOURNAL%20OF%20MOLECULAR%20SCIENCES%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B20%26lt%3B%5C%2Fi%26gt%3B%20%2815%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms20153803%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms20153803%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Arabidopsis%20Trichome%20Contains%20Two%20Plasma%20Membrane%20Domains%20with%20Different%20Lipid%20Compositions%20Which%20Attract%20Distinct%20EXO70%20Subunits%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Z.%22%2C%22lastName%22%3A%22Kub%5Cu00e1tov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Pejchar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Potocky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Sekeres%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Z%5Cu00e1rsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22I.%22%2C%22lastName%22%3A%22Kulich%22%7D%5D%2C%22abstractNote%22%3A%22Plasma%20membrane%20%28PM%29%20lipid%20composition%20and%20domain%20organization%20are%20modulated%20by%20polarized%20exocytosis.%20Conversely%2C%20targeting%20of%20secretory%20vesicles%20at%20specific%20domains%20in%20the%20PM%20is%20carried%20out%20by%20exocyst%20complexes%2C%20which%20contain%20EXO70%20subunits%20that%20play%20a%20significant%20role%20in%20the%20final%20recognition%20of%20the%20target%20membrane.%20As%20we%20have%20shown%20previously%2C%20a%20mature%20Arabidopsis%20trichome%20contains%20a%20basal%20domain%20with%20a%20thin%20cell%20wall%20and%20an%20apical%20domain%20with%20a%20thick%20secondary%20cell%20wall%2C%20which%20is%20developed%20in%20an%20EXO70H4-dependent%20manner.%20These%20domains%20are%20separated%20by%20a%20cell%20wall%20structure%20named%20the%20Ortmannian%20ring.%20Using%20phospholipid%20markers%2C%20we%20demonstrate%20that%20there%20are%20two%20distinct%20PM%20domains%20corresponding%20to%20these%20cell%20wall%20domains.%20The%20apical%20domain%20is%20enriched%20in%20phosphatidic%20acid%20%28PA%29%20and%20phosphatidylserine%2C%20with%20an%20undetectable%20amount%20of%20phosphatidylinositol%204%2C5-bisphosphate%20%28PIP2%29%2C%20whereas%20the%20basal%20domain%20is%20PIP2-rich.%20While%20the%20apical%20domain%20recruits%20EXO70H4%2C%20the%20basal%20domain%20recruits%20EXO70A1%2C%20which%20corresponds%20to%20the%20lipid-binding%20capacities%20of%20these%20two%20paralogs.%20Loss%20of%20EXO70H4%20results%20in%20a%20loss%20of%20the%20Ortmannian%20ring%20border%20and%20decreased%20apical%20PA%20accumulation%2C%20which%20causes%20the%20PA%20and%20PIP2%20domains%20to%20merge%20together.%20Using%20transmission%20electron%20microscopy%2C%20we%20describe%20these%20accumulations%20as%20a%20unique%20anatomical%20feature%20of%20the%20apical%20cell%20wall-radially%20distributed%20rod-shaped%20membranous%20pockets%2C%20where%20both%20EXO70H4%20and%20lipid%20markers%20are%20immobilized.%22%2C%22date%22%3A%222019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3390%5C%2Fijms20153803%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221422-0067%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A36Z%22%7D%7D%2C%7B%22key%22%3A%22SUWBZZPS%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Schwarzerov%5Cu00e1%20et%20al.%22%2C%22parsedDate%22%3A%222019%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BSchwarzerov%26%23xE1%3B%2C%20K.%3B%20Bellinvia%2C%20E.%3B%20Martinek%2C%20J.%3B%20Sikorov%26%23xE1%3B%2C%20L.%3B%20Dostal%2C%20V.%3B%20Libusov%26%23xE1%3B%2C%20L.%3B%20Bokvaj%2C%20P.%3B%20Fischer%2C%20L.%3B%20Schmit%2C%20A.%20C.%3B%20Nick%2C%20P.%20%26lt%3Bb%26gt%3BTubulin%20Is%20Actively%20Exported%20from%20the%20Nucleus%20through%20the%20Exportin1%5C%2FCRM1%20Pathway%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BSCIENTIFIC%20REPORTS%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B9%26lt%3B%5C%2Fi%26gt%3B.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-019-42056-6%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-019-42056-6%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Tubulin%20is%20actively%20exported%20from%20the%20nucleus%20through%20the%20Exportin1%5C%2FCRM1%20pathway%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Schwarzerov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Bellinvia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Martinek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Sikorov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Dostal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Libusov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Bokvaj%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Fischer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20C.%22%2C%22lastName%22%3A%22Schmit%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Nick%22%7D%5D%2C%22abstractNote%22%3A%22Microtubules%20of%20all%20eukaryotic%20cells%20are%20formed%20by%20alpha-and%20beta-tubulin%20heterodimers.%20In%20addition%20to%20the%20well%20known%20cytoplasmic%20tubulins%2C%20a%20subpopulation%20of%20tubulin%20can%20occur%20in%20the%20nucleus.%20So%20far%2C%20the%20potential%20function%20of%20nuclear%20tubulin%20has%20remained%20elusive.%20In%20this%20work%2C%20we%20show%20that%20alpha-and%20beta-tubulins%20of%20various%20organisms%20contain%20multiple%20conserved%20nuclear%20export%20sequences%2C%20which%20are%20potential%20targets%20of%20the%20Exportin%201%5C%2FCRM1%20pathway.%20We%20demonstrate%20exemplarily%20that%20these%20NES%20motifs%20are%20sufficient%20to%20mediate%20export%20of%20GFP%20as%20model%20cargo%20and%20that%20this%20export%20can%20be%20inhibited%20by%20leptomycin%20B%2C%20an%20inhibitor%20of%20the%20Exportin%201%5C%2FCRM1%20pathway.%20Likewise%2C%20leptomycin%20B%20causes%20accumulation%20of%20GFP-tagged%20tubulin%20in%20interphase%20nuclei%2C%20in%20both%20plant%20and%20animal%20model%20cells.%20Our%20analysis%20of%20nuclear%20tubulin%20content%20supports%20the%20hypothesis%20that%20an%20important%20function%20of%20nuclear%20tubulin%20export%20is%20the%20exclusion%20of%20tubulin%20from%20interphase%20nuclei%2C%20after%20being%20trapped%20by%20nuclear%20envelope%20reassembly%20during%20telophase.%22%2C%22date%22%3A%222019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-019-42056-6%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%222045-2322%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A36Z%22%7D%7D%2C%7B%22key%22%3A%22NEBUVSGF%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Princov%5Cu00e1%20et%20al.%22%2C%22parsedDate%22%3A%222019%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BPrincov%26%23xE1%3B%2C%20J.%3B%20Sch%26%23xE4%3Btz%2C%20M.%3B%20Tupa%2C%20O.%3B%20Prevorovsky%2C%20M.%20%26lt%3Bb%26gt%3BAnalysis%20of%20Lipid%20Droplet%20Content%20in%20Fission%20and%20Budding%20Yeasts%20Using%20Automated%20Image%20Processing%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BJOVE-JOURNAL%20OF%20VISUALIZED%20EXPERIMENTS%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20No.%20149.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3791%5C%2F59889%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3791%5C%2F59889%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Analysis%20of%20Lipid%20Droplet%20Content%20in%20Fission%20and%20Budding%20Yeasts%20using%20Automated%20Image%20Processing%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Princov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Sch%5Cu00e4tz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Tupa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Prevorovsky%22%7D%5D%2C%22abstractNote%22%3A%22Lipid%20metabolism%20and%20its%20regulation%20are%20of%20interest%20to%20both%20basic%20and%20applied%20life%20sciences%20and%20biotechnology.%20In%20this%20regard%2C%20various%20yeast%20species%20are%20used%20as%20models%20in%20lipid%20metabolic%20research%20or%20for%20industrial%20lipid%20production.%20Lipid%20droplets%20are%20highly%20dynamic%20storage%20bodies%20and%20their%20cellular%20content%20represents%20a%20convenient%20readout%20of%20the%20lipid%20metabolic%20state.%20Fluorescence%20microscopy%20is%20a%20method%20of%20choice%20for%20quantitative%20analysis%20of%20cellular%20lipid%20droplets%2C%20as%20it%20relies%20on%20widely%20available%20equipment%20and%20allows%20analysis%20of%20individual%20lipid%20droplets.%20Furthermore%2C%20microscopic%20image%20analysis%20can%20be%20automated%2C%20greatly%20increasing%20overall%20analysis%20throughput.%20Here%2C%20we%20describe%20an%20experimental%20and%20analytical%20workflow%20for%20automated%20detection%20and%20quantitative%20description%20of%20individual%20lipid%20droplets%20in%20three%20different%20model%20yeast%20species%3A%20the%20fission%20yeasts%20Schizosaccharomyces%20pombe%20and%20Schizosaccharomyces%20japonicus%2C%20and%20the%20budding%20yeast%20Saccharomyces%20cerevisiae.%20Lipid%20droplets%20are%20visualized%20with%20BODIPY%20493%5C%2F503%2C%20and%20cell-impermeable%20fluorescent%20dextran%20is%20added%20to%20the%20culture%20media%20to%20help%20identify%20cell%20boundaries.%20Cells%20are%20subjected%20to%203D%20epifluorescence%20microscopy%20in%20green%20and%20blue%20channels%20and%20the%20resulting%20z-stack%20images%20are%20processed%20automatically%20by%20a%20MATLAB%20pipeline.%20The%20procedure%20outputs%20rich%20quantitative%20data%20on%20cellular%20lipid%20droplet%20content%20and%20individual%20lipid%20droplet%20characteristics%20in%20a%20tabular%20format%20suitable%20for%20downstream%20analyses%20in%20major%20spreadsheet%20or%20statistical%20packages.%20We%20provide%20example%20analyses%20of%20lipid%20droplet%20content%20under%20various%20conditions%20that%20affect%20cellular%20lipid%20metabolism.%22%2C%22date%22%3A%222019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3791%5C%2F59889%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221940-087X%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A36Z%22%7D%7D%2C%7B%22key%22%3A%22ZUFJJ9R3%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Fawley%20et%20al.%22%2C%22parsedDate%22%3A%222019%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BFawley%2C%20M.%20W.%3B%20Nemcova%2C%20Y.%3B%20Fawley%2C%20K.%20P.%20%26lt%3Bb%26gt%3BPhylogeny%20and%20Characterization%20of%20%26lt%3Bi%26gt%3BParaeustigmatos%20Columelliferus%26lt%3B%5C%2Fi%26gt%3B%2C%20Gen.%20et%20Sp.%20Nov.%2C%20a%20Member%20of%20the%20Eustigmatophyceae%20That%20May%20Represent%20a%20Basal%20Group%20within%20the%20Eustigmatales%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BFOTTEA%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B19%26lt%3B%5C%2Fi%26gt%3B%20%282%29%2C%20107%26%23x2013%3B114.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.5507%5C%2Ffot.2019.002%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.5507%5C%2Ffot.2019.002%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Phylogeny%20and%20characterization%20of%20%3Ci%3EParaeustigmatos%20columelliferus%3C%5C%2Fi%3E%2C%20gen.%20et%20sp.%20nov.%2C%20a%20member%20of%20the%20Eustigmatophyceae%20that%20may%20represent%20a%20basal%20group%20within%20the%20Eustigmatales%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20W.%22%2C%22lastName%22%3A%22Fawley%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Y.%22%2C%22lastName%22%3A%22Nemcova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20P.%22%2C%22lastName%22%3A%22Fawley%22%7D%5D%2C%22abstractNote%22%3A%22The%20new%20taxon%2C%20Paraeustigmatos%20columelliferus%2C%20was%20isolated%20from%20a%20mat%20of%20the%20filamentous%20alga%20Zygnema%20on%20the%20shore%20of%20Lake%20Monticello%20in%20Arkansas%2C%20USA.%20The%20results%20of%20the%20phylogenetic%20analysis%20of%2018S%20rDNA%20and%20rbcL%20sequence%20data%20suggest%20that%20this%20alga%20is%20likely%20allied%20with%20the%20Eustigmatales%3B%20alternately%2C%20it%20may%20represent%20a%20new%20third%20linage%20of%20the%20class.%20Light%20and%20electron%20microscopy%20reveal%20cellular%20features%20similar%20to%20other%20members%20of%20the%20Eustigmatophyceae%3B%20however%2C%20the%20cell%20wall%20bears%20unusual%20structures%20that%20resemble%20the%20columellae%20of%20the%20walls%20of%20pollen%20grains.%22%2C%22date%22%3A%222019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.5507%5C%2Ffot.2019.002%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221802-5439%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%22AHFJLCYP%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Stundlov%5Cu00e1%20et%20al.%22%2C%22parsedDate%22%3A%222019%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BStundlov%26%23xE1%3B%2C%20J.%3B%20Sm%26%23xED%3Bd%2C%20J.%3B%20Nguyen%2C%20P.%3B%20St%26%23xE1%3Bhlavsky%2C%20F.%20%26lt%3Bb%26gt%3BCryptic%20Diversity%20and%20Dynamic%20Chromosome%20Evolution%20in%20Alpine%20Scorpions%20%28Euscorpiidae%26lt%3Bi%26gt%3B%3A%20Euscorpius%26lt%3B%5C%2Fi%26gt%3B%29%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BMOLECULAR%20PHYLOGENETICS%20AND%20EVOLUTION%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B134%26lt%3B%5C%2Fi%26gt%3B%2C%20152%26%23x2013%3B163.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.ympev.2019.02.002%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.ympev.2019.02.002%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Cryptic%20diversity%20and%20dynamic%20chromosome%20evolution%20in%20Alpine%20scorpions%20%28Euscorpiidae%3Ci%3E%3A%20Euscorpius%3C%5C%2Fi%3E%29%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Stundlov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Sm%5Cu00edd%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Nguyen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22St%5Cu00e1hlavsky%22%7D%5D%2C%22abstractNote%22%3A%22Over%20time%2C%20mountain%20biota%20has%20undergone%20complex%20evolutionary%20histories%20that%20have%20left%20imprints%20on%20its%20genomic%20arrangement%2C%20geographical%20distribution%20and%20diversity%20of%20contemporary%20lineages.%20Knowledge%20on%20these%20biogeographical%20aspects%20still%20lags%20behind%20for%20invertebrates%20inhabiting%20the%20Alpine%20region.%20In%20the%20present%20study%2C%20we%20examined%20three%20scorpion%20species%20of%20the%20subgenus%20Euscorpius%20%28Alpiscorpius%29%20from%20the%20European%20Alps%20using%20cytogenetic%20and%20molecular%20phylogenetic%20approaches%20to%20determine%20the%20variation%20and%20population%20structure%20of%20extant%20lineages%20at%20both%20chromosome%20and%20genetic%20level%2C%20and%20to%20provide%20an%20insight%20into%20the%20species%20diversification%20histories.%20We%20detected%20considerable%20intraspecific%20variability%20in%20chromosome%20complements%20and%20localization%20of%20the%2018S%20rDNA%20loci%20in%20all%20studied%20species.%20Such%20chromosome%20differences%20were%20noticeable%20as%20the%20existence%20of%20three%20%5Bin%20E.%20%28A.%29%20alpha%20and%20E.%20%28A.%29%20germanus%5D%20or%20four%20%5Bin%20E.%20%28A.%29%20gamma%5D%20range-restricted%20karyotypic%20races.%20These%20races%20differed%20from%20one%20another%20either%20by%202n%20%5Bin%20E.%20%28A.%29%20alpha%202n%20%3D%2054%2C%2060%2C%2090%3B%20in%20E.%20%28A.%29%20gamma%202n%20%3D%2058%2C%2060%2C%2088%2C%2086-92%5D%2C%20or%20by%20the%20karyotypic%20formula%20%5Bin%20E.%20%28A.%29%20germanus%202n%20%3D%2034m%20%2B%2012sm%3B%2036m%20%2B%201%20Osm%3B%2042m%20%2B%204sm%5D.%20Using%20mitochondrial%20%2816S%20rRNA%2C%20COI%29%20and%20nuclear%20%2828S%20rDNA%29%20genetic%20markers%2C%20we%20examined%20genetic%20variation%20and%20reconstructed%20phylogenetic%20relationships%20among%20the%20karyotypic%20races.%20Both%20approaches%20provided%20evidence%20for%20the%20existence%20of%20ten%20deeply%20divergent%20lineages%20exhibiting%20the%20features%20of%20local%20endemics%20and%20indicating%20the%20presence%20of%20cryptic%20species.%20Molecular%20dating%20analyses%20suggest%20that%20these%20lineages%20diversified%20during%20the%20Plio-Pleistocene%20and%20this%20process%20was%20presumably%20accompanied%20by%20dynamic%20structural%20changes%20in%20the%20genome%20organization.%22%2C%22date%22%3A%222019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.ympev.2019.02.002%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221055-7903%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%22Q87H5AEI%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hernychova%20et%20al.%22%2C%22parsedDate%22%3A%222019%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BHernychova%2C%20L.%3B%20Rosulek%2C%20M.%3B%20K%26%23xE1%3Bdek%2C%20A.%3B%20Mareska%2C%20V.%3B%20Chmel%26%23xED%3Bk%2C%20J.%3B%20Ad%26%23xE1%3Bmkov%26%23xE1%3B%2C%20L.%3B%20Grob%26%23xE1%3Brov%26%23xE1%3B%2C%20V.%3B%20Sebesta%2C%20O.%3B%20Kukacka%2C%20Z.%3B%20Sk%26%23xE1%3Bla%2C%20K.%3B%20Spiwok%2C%20V.%3B%20Cerny%2C%20J.%3B%20Nov%26%23xE1%3Bk%2C%20P.%20%26lt%3Bb%26gt%3BThe%20C-Type%20Lectin-like%20Receptor%20Nkrp1b%3A%20Structural%20Proteomics%20Reveals%20Features%20Affecting%20Protein%20Conformation%20and%20Interactions%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BJOURNAL%20OF%20PROTEOMICS%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B196%26lt%3B%5C%2Fi%26gt%3B%2C%20162%26%23x2013%3B172.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jprot.2018.11.007%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jprot.2018.11.007%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20C-type%20lectin-like%20receptor%20Nkrp1b%3A%20Structural%20proteomics%20reveals%20features%20affecting%20protein%20conformation%20and%20interactions%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Hernychova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Rosulek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22K%5Cu00e1dek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Mareska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Chmel%5Cu00edk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Ad%5Cu00e1mkov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Grob%5Cu00e1rov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Sebesta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Z.%22%2C%22lastName%22%3A%22Kukacka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Sk%5Cu00e1la%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Spiwok%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Cerny%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Nov%5Cu00e1k%22%7D%5D%2C%22abstractNote%22%3A%22The%20cytotoxicity%20of%20mouse%20natural%20killer%20%28NK%29%20cells%20in%20response%20to%20pathological%20changes%20in%20target%20cells%20is%20regulated%20via%20the%20Nkrp1b%20receptor.%20Here%2C%20we%20characterized%20the%20Nkrp1b%20structure%20and%20structural%20features%20%28stalk%2C%20loop%2C%20and%20oligomerization%20state%29%20that%20affect%20its%20interactions.%20To%20study%20the%20Nkrp1b%20protein%20structure%20and%20the%20functional%20importance%20of%20its%20stalk%2C%20two%20Nkrp1b%20protein%20variants%20differing%20by%20the%20presence%20of%20the%20stalk%20were%20prepared.%20These%20variants%20were%20studied%20using%20a%20combination%20of%20structural%20mass%20spectrometry%20approaches%20with%20computational%20modeling%20to%20derive%20structural%20models.%20In%20addition%2C%20information%20about%20biological%20activity%20and%20localization%20in%20mammalian%20cells%20was%20acquired%20using%20scanning%20microscopy%20techniques%20and%20western%20blotting.%20Based%20on%20these%20methods%2C%20we%20obtained%20the%20structure%20of%20Nkrp1b%20ectodomain%20in%20its%20monomeric%20and%20dimeric%20conformations%2C%20identified%20the%20dimerization%20interface%2C%20and%20determined%20disulfide%20connections%20within%20the%20molecule.%20We%20found%20that%20Nkrp1b%20occurs%20as%20a%20mixture%20of%20monomers%20and%20homodimers%2C%20both%20in%20vitro%20and%20in%20vivo.%20Significance%3A%20Despite%20the%20long-standing%20assumption%20that%20Nkrp1%20proteins%20are%20homodimers%20connected%20by%20disulfide%20bonds%20in%20the%20stalk%20region%2C%20our%20data%20showed%20that%20both%20Nkrp1b%20protein%20variants%20form%20monomers%20and%20homodimers%20irrespective%20of%20the%20presence%20of%20the%20stalk.%20We%20demonstrated%20that%20the%20stalk%20is%20not%20crucial%20for%20protein%20dimerization%20or%20ligand%20binding%20and%20that%20Nkrp1b%20interacts%20with%20its%20natural%20ligands%20only%20in%20its%20monomeric%20conformation%3B%20therefore%2C%20dimers%20may%20have%20another%20regulatory%20function.%20Using%20a%20unique%20combination%20of%20computational%2C%20biochemical%2C%20and%20biological%20methods%2C%20we%20revealed%20the%20structural%20conformation%20and%20behavior%20of%20Nkrp1b%20in%20its%20native%20state.%20In%20addition%2C%20it%20is%20a%20first%20report%20utilizing%20the%20intermolecular%20chemical%20cross-linking%20of%20light-%20and%20heavy-labeled%20protein%20chains%20together%20with%20ion%20mobility-mass%20spectrometry%20to%20design%20the%20structural%20models%20of%20protein%20homodimers.%22%2C%22date%22%3A%222019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.jprot.2018.11.007%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221874-3919%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A35Z%22%7D%7D%2C%7B%22key%22%3A%22UDHD7VUZ%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Frolikova%20et%20al.%22%2C%22parsedDate%22%3A%222019%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BFrolikova%2C%20M.%3B%20Valaskova%2C%20E.%3B%20Cerny%2C%20J.%3B%20Lumeau%2C%20A.%3B%20Sebkova%2C%20N.%3B%20Palenikova%2C%20V.%3B%20Sanchez-Hernandez%2C%20N.%3B%20Pohlova%2C%20A.%3B%20Manaskova-Postlerova%2C%20P.%3B%20Dvorakova-Hortova%2C%20K.%20%26lt%3Bb%26gt%3BAddressing%20the%20Compartmentalization%20of%20Specific%20Integrin%20Heterodimers%20in%20Mouse%20Sperm%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BINTERNATIONAL%20JOURNAL%20OF%20MOLECULAR%20SCIENCES%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B20%26lt%3B%5C%2Fi%26gt%3B%20%285%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms20051004%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms20051004%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Addressing%20the%20Compartmentalization%20of%20Specific%20Integrin%20Heterodimers%20in%20Mouse%20Sperm%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michaela%22%2C%22lastName%22%3A%22Frolikova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eliska%22%2C%22lastName%22%3A%22Valaskova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jiri%22%2C%22lastName%22%3A%22Cerny%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Audrey%22%2C%22lastName%22%3A%22Lumeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Natasa%22%2C%22lastName%22%3A%22Sebkova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Veronika%22%2C%22lastName%22%3A%22Palenikova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Noemi%22%2C%22lastName%22%3A%22Sanchez-Hernandez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alzbeta%22%2C%22lastName%22%3A%22Pohlova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pavla%22%2C%22lastName%22%3A%22Manaskova-Postlerova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katerina%22%2C%22lastName%22%3A%22Dvorakova-Hortova%22%7D%5D%2C%22abstractNote%22%3A%22Integrins%20are%20transmembrane%20cell%20receptors%20involved%20in%20two%20crucial%20mechanisms%20for%20successful%20fertilization%2C%20namely%2C%20mammalian%20intracellular%20signaling%20and%20cell%20adhesion.%20Integrins%2064%2C%2031%20and%2061%20are%20three%20major%20laminin%20receptors%20expressed%20on%20the%20surface%20of%20mammalian%20cells%20including%20gametes%2C%20and%20the%20presence%20of%20individual%20integrin%20subunits%203%2C%206%2C%201%20and%204%20has%20been%20previously%20detected%20in%20mammalian%20sperm.%20However%2C%20to%20date%2C%20proof%20of%20the%20existence%20of%20individual%20heterodimer%20pairs%20in%20sperm%20and%20their%20detailed%20localization%20is%20missing.%20The%20major%20conclusion%20of%20this%20study%20is%20evidence%20that%20the%204%20integrin%20subunit%20is%20expressed%20in%20mouse%20sperm%20and%20that%20it%20pairs%20with%20subunit%206%3B%20additionally%2C%20there%20is%20a%20detailed%20identification%20of%20integrin%20heterodimer%20pairs%20across%20individual%20membranes%20in%20an%20intact%20mouse%20sperm%20head.%20We%20also%20demonstrate%20the%20existence%20of%204%20integrin%20mRNAs%20in%20round%20spermatids%20and%20spermatogonia%20by%20q-RT-PCR%2C%20which%20was%20further%20supported%20by%20sequencing%20the%20PCR%20products.%20Using%20super-resolution%20microscopy%20accompanied%20by%20colocalization%20analysis%2C%20we%20located%20integrin%20subunits%20as%20follows%3A%206%5C%2F4-inner%20apical%20acrosomal%20membrane%20and%20equatorial%20segment%3B%203%2C%206%5C%2F1%2C%204-plasma%20membrane%20overlaying%20the%20apical%20acrosome%3B%20and%203%5C%2F1-outer%20acrosomal%20membrane.%20The%20existence%20of%2064%2C%2031%20and%2061%20heterodimers%20was%20further%20confirmed%20by%20proximity%20ligation%20assay%20%28PLA%29.%20In%20conclusion%2C%20we%20delivered%20detailed%20characterization%20of%203%2C%206%2C%201%20and%204%20integrin%20subunits%2C%20showing%20their%20presence%20in%20distinct%20compartments%20of%20the%20intact%20mouse%20sperm%20head.%20Moreover%2C%20we%20identified%20sperm-specific%20localization%20for%20heterodimers%2064%2C%2031%20and%2061%2C%20and%20their%20membrane%20compartmentalization%20and%20the%20presented%20data%20show%20a%20complexity%20of%20membranes%20overlaying%20specialized%20microdomain%20structures%20in%20the%20sperm%20head.%20Their%20different%20protein%20compositions%20of%20these%20individual%20membrane%20rafts%20may%20play%20a%20specialized%20role%2C%20based%20on%20their%20involvement%20in%20sperm-epithelium%20and%20sperm-egg%20interaction.%22%2C%22date%22%3A%222019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3390%5C%2Fijms20051004%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221422-0067%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A33Z%22%7D%7D%2C%7B%22key%22%3A%22ZV2PB5AU%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Batelka%20et%20al.%22%2C%22parsedDate%22%3A%222019%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BBatelka%2C%20J.%3B%20Prokop%2C%20J.%3B%20Pohl%2C%20H.%3B%20Bai%2C%20M.%3B%20Zhang%2C%20W.%3B%20Beutel%2C%20R.%20G.%20%26lt%3Bb%26gt%3BHighly%20Specialized%20Cretaceous%20Beetle%20Parasitoids%20%28Ripiphoridae%29%20Identified%20with%20Optimized%20Visualization%20of%20Microstructures%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BSYSTEMATIC%20ENTOMOLOGY%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B44%26lt%3B%5C%2Fi%26gt%3B%20%282%29%2C%20396%26%23x2013%3B407.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fsyen.12331%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fsyen.12331%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Highly%20specialized%20Cretaceous%20beetle%20parasitoids%20%28Ripiphoridae%29%20identified%20with%20optimized%20visualization%20of%20microstructures%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jan%22%2C%22lastName%22%3A%22Batelka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jakub%22%2C%22lastName%22%3A%22Prokop%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hans%22%2C%22lastName%22%3A%22Pohl%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ming%22%2C%22lastName%22%3A%22Bai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Weiwei%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rolf%20G.%22%2C%22lastName%22%3A%22Beutel%22%7D%5D%2C%22abstractNote%22%3A%22Extremely%20miniaturized%20longipedes%20insects%20%28body%20length%20c.%200.3%20mm%29%20embedded%20in%20two%20pieces%20of%20Cretaceous%20amber%20from%20Myanmar%20are%20described%20and%20interpreted.%20Using%20inverted%20fluorescence%20and%20light%20microscopy%20for%20detailed%20analysis%20of%20microstructures%2C%20the%20inclusions%20were%20identified%20as%20primary%20larvae%20of%20the%20beetle%20family%20Ripiphoridae%2C%20subfamily%20Ripidiinae.%20While%20the%20structure%20of%20thoracic%20and%20abdominal%20segments%20including%20appendages%20corresponds%20well%20with%20the%20groundplan%20known%20in%20recent%20members%20of%20Ripidiinae%2C%20a%20curved%20prosternal%20ridge%20with%20prominent%20spines%20%28each%20c.%205%20mu%20m%29%2C%20the%20reduced%20condition%20of%20stemmata%20and%20antennae%20and%20the%20lack%20of%20sharp%20mandibles%20are%20unique%20features%20within%20the%20entire%20family%2C%20apparently%20apomorphies%20of%20the%20longipedes%20larvae.%20A%20sinuate%20prosternal%20edge%20with%20a%20dense%20row%20of%20spines%20%28prosternoctenidium%29%20might%20be%20homologous%20with%20%60head%20ctenidia%26%23039%3B%20in%20some%20previously%20described%20miniaturized%20conicocephalate%20larvae%2C%20but%20further%20investigation%20is%20needed.%20The%20morphological%20differences%20between%20the%20head%20of%20longipedes%20larvae%20and%20extant%20Ripidiinae%20are%20interpreted%20as%20adaptations%20to%20different%20groups%20of%20hosts%20and%20life%20strategies.%20Palaeoethology%20of%20the%20longipedes%20larvae%20is%20briefly%20discussed.%20In%20addition%2C%20the%20systematic%20placement%20of%20conicocephalate%20larvae%20from%20Canadian%2C%20Myanmar%20and%20Russian%20Cretaceous%20ambers%2C%20already%20interpreted%20by%20various%20authors%20as%20primary%20instars%20within%20Coleopterida%20%28assigned%20to%20either%20Strepsiptera%20or%20to%20the%20coleopteran%20Tenebrionoidea%3A%20Ripiphoridae%29%2C%20is%20discussed.%22%2C%22date%22%3A%222019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2Fsyen.12331%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220307-6970%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A33Z%22%7D%7D%2C%7B%22key%22%3A%2273A5JGDY%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Nedvedova%20et%20al.%22%2C%22parsedDate%22%3A%222019%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BNedvedova%2C%20I.%3B%20Kolar%2C%20D.%3B%20Neckar%2C%20J.%3B%20Kalous%2C%20M.%3B%20Pravenec%2C%20M.%3B%20Silhavil%2C%20J.%3B%20Korenkova%2C%20W.%3B%20Kolar%2C%20F.%3B%20Zurmanova%2C%20J.%20M.%20%26lt%3Bb%26gt%3BCardioprotective%20Regimen%20of%20Adaptation%20to%20Chronic%20Hypoxia%20Diversely%20Alters%20Myocardial%20Gene%20Expression%20in%20SHR%20and%20SHR-Mt%28BN%29%20Conplastic%20Rat%20Strains%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BFRONTIERS%20IN%20ENDOCRINOLOGY%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B9%26lt%3B%5C%2Fi%26gt%3B.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffendo.2018.00809%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffendo.2018.00809%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Cardioprotective%20Regimen%20of%20Adaptation%20to%20Chronic%20Hypoxia%20Diversely%20Alters%20Myocardial%20Gene%20Expression%20in%20SHR%20and%20SHR-mt%28BN%29%20Conplastic%20Rat%20Strains%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Iveta%22%2C%22lastName%22%3A%22Nedvedova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Kolar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jan%22%2C%22lastName%22%3A%22Neckar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22Kalous%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michel%22%2C%22lastName%22%3A%22Pravenec%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jan%22%2C%22lastName%22%3A%22Silhavil%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Waste%22%2C%22lastName%22%3A%22Korenkova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Frantisek%22%2C%22lastName%22%3A%22Kolar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jitka%20M.%22%2C%22lastName%22%3A%22Zurmanova%22%7D%5D%2C%22abstractNote%22%3A%22Adaptation%20to%20continuous%20normobaric%20hypoxia%20%28CNH%29%20protects%20the%20heart%20against%20acute%20ischemia%5C%2Freperfusion%20injury.%20Recently%2C%20we%20have%20demonstrated%20the%20infarct%20size-limiting%20effect%20of%20CNH%20also%20in%20hearts%20of%20spontaneously%20hypertensive%20rats%20%28SHR%29%20and%20in%20conplastic%20SHR-mt%28BN%29%20strain%20characterized%20by%20the%20selective%20replacement%20of%20the%20mitochondrial%20genome%20of%20SHR%20with%20that%20of%20more%20ischemia-resistant%20Brown%20Norway%20rats.%20Importantly%2C%20cardioprotective%20effect%20of%20CNH%20was%20more%20pronounced%20in%20SHR-mt%28BN%29%20than%20in%20SHR.%20Thus%2C%20here%20we%20aimed%20to%20identify%20candidate%20genes%20which%20may%20contribute%20to%20this%20difference%20between%20the%20strains.%20Rats%20were%20adapted%20to%20CNH%20%28FiO%282%29%200.1%29%20for%203%20weeks%20or%20kept%20at%20room%20air%20as%20normoxic%20controls.%20Screening%20of%2045%20transcripts%20was%20performed%20in%20left%20ventricles%20using%20Biomark%20Chip.%20Significant%20differences%20between%20the%20groups%20were%20analyzed%20by%20univariate%20analysis%20%28ANOVA%29%20and%20the%20genes%20contributing%20to%20the%20differences%20between%20the%20strains%20unmasked%20by%20CNH%20were%20identified%20by%20multivariate%20analyses%20%28PCA%2C%20SOM%29.%20ANOVA%20with%20Bonferroni%20correction%20revealed%20that%20transcripts%20differently%20affected%20by%20CNH%20in%20SHR%20and%20SHR-mt%28BN%29%20belong%20predominantly%20to%20lipid%20metabolism%20and%20antioxidant%20defense.%20PCA%20divided%20four%20experimental%20groups%20into%20two%20main%20clusters%20corresponding%20to%20chronically%20hypoxic%20and%20normoxic%20groups%2C%20and%20differences%20between%20the%20strains%20were%20more%20pronounced%20after%20CNH.%20Subsequently%2C%20the%20following%2014%20candidate%20transcripts%20were%20selected%20by%20PCA%2C%20and%20confirmed%20by%20SOM%20analyses%2C%20that%20can%20contribute%20to%20the%20strain%20differences%20in%20cardioprotective%20phenotype%20afforded%20by%20CNH%3A%20Alkaline%20ceramidase%202%20%28Acer2%29%2C%20Fatty%20acid%20translocase%20%28Cd36%29%2C%20Aconitase%201%20%28Aco1%29%2C%20Peroxisome%20proliferator%20activated%20receptor%20gamma%20%28Pparg%29%2C%20Hemoxygenase%202%20%28Hmox2%29%2C%20Phospholipase%20A2%20group%20IIA%20%28Ppla2g2a%29%2C%20Dynarnin-related%20protein%20%28Drp%29%2C%20Protein%20kinase%20C%20epsilon%20%28Pkce%29%2C%20Hexokinase%202%20%28Hk2%29%2C%20Sphingomyelin%20synthase%202%20%28Sgms2%29%2C%20Caspase%203%20%28Casp3%29%2C%20Mitofussin%201%20%28Mfn1%29%2C%20Phospholipase%20A2%20group%20V%20%28Pla2g5%29%2C%20and%20Catalase%20%28Cat%29.%20Our%20data%20suggest%20that%20the%20stronger%20cardioprotective%20phenotype%20of%20conplastic%20SHR-mt%28BN%29%20strain%20afforded%20by%20CNH%20is%20associated%20with%20either%20preventing%20the%20drop%20or%20increasing%20the%20expression%20of%20transcripts%20related%20to%20energy%20metabolism%2C%20antioxidant%20response%20and%20mitochondrial%20dynamics.%22%2C%22date%22%3A%222019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.3389%5C%2Ffendo.2018.00809%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221664-2392%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A33Z%22%7D%7D%2C%7B%22key%22%3A%22VJBAWRAG%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Figura%20et%20al.%22%2C%22parsedDate%22%3A%222019%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BFigura%2C%20T.%3B%20Tylov%26%23xE1%3B%2C%20E.%3B%20Soch%2C%20J.%3B%20Selosse%2C%20M.%20A.%3B%20Ponert%2C%20J.%20%26lt%3Bb%26gt%3B%26lt%3Bi%26gt%3BIn%20Vitro%26lt%3B%5C%2Fi%26gt%3B%20Axenic%20Germination%20and%20Cultivation%20of%20Mixotrophic%20Pyroloideae%20%28Ericaceae%29%20and%20Their%20Post-Germination%20Ontogenetic%20Development%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BANNALS%20OF%20BOTANY%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2019%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B123%26lt%3B%5C%2Fi%26gt%3B%20%284%29%2C%20625%26%23x2013%3B639.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Faob%5C%2Fmcy195%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Faob%5C%2Fmcy195%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22%3Ci%3EIn%20vitro%3C%5C%2Fi%3E%20axenic%20germination%20and%20cultivation%20of%20mixotrophic%20Pyroloideae%20%28Ericaceae%29%20and%20their%20post-germination%20ontogenetic%20development%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%22%2C%22lastName%22%3A%22Figura%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Tylov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Soch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20A.%22%2C%22lastName%22%3A%22Selosse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Ponert%22%7D%5D%2C%22abstractNote%22%3A%22Background%20and%20Aims%20Pyroloids%2C%20forest%20sub-shrubs%20of%20the%20Ericaceae%20family%2C%20are%20an%20important%20model%20for%20their%20mixotrophic%20nutrition%2C%20which%20mixes%20carbon%20from%20photosynthesis%20and%20from%20their%20mycorrhizal%20fungi.%20They%20have%20medical%20uses%20but%20are%20difficult%20to%20cultivate%20ex%20situ%3B%20in%20particular%2C%20their%20dust%20seeds%20contain%20undifferentiated%2C%20few-celled%20embryos%2C%20whose%20germination%20is%20normally%20fully%20supported%20by%20fungal%20partners.%20Their%20germination%20and%20early%20ontogenesis%20thus%20remain%20elusive.%20Methods%20An%20optimized%20in%20vitro%20cultivation%20system%20of%20five%20representatives%20from%20the%20subfamily%20Pyroloideae%20was%20developed%20to%20study%20the%20strength%20of%20seed%20dormancy%20and%20the%20effect%20of%20different%20media%20and%20conditions%20%28including%20light%2C%20gibberellins%20and%20soluble%20saccharides%29%20on%20germination.%20The%20obtained%20plants%20were%20analysed%20for%20morphological%2C%20anatomical%20and%20histochemical%20development.%20Key%20Results%20Thanks%20to%20this%20novel%20cultivation%20method%2C%20which%20breaks%20dormancy%20and%20achieved%20up%20to%20100%20%25%20germination%2C%20leafy%20shoots%20were%20obtained%20in%20vitro%20for%20representatives%20of%20all%20pyroloid%20genera%20%28Moneses%2C%20Orthilia%2C%20Pyrola%20and%20Chimaphila%29.%20In%20all%20cases%2C%20the%20first%20post-germination%20stage%20is%20an%20undifferentiated%20structure%2C%20from%20which%20a%20root%20meristem%20later%20emerges%2C%20well%20before%20formation%20of%20an%20adventive%20shoot.%20Conclusions%20This%20cultivation%20method%20can%20be%20used%20for%20further%20research%20or%20for%20ex%20situ%20conservation%20of%20pyroloid%20species.%20After%20strong%20seed%20dormancy%20is%20broken%2C%20the%20tiny%20globular%20embryo%20of%20pyroloids%20germinates%20into%20an%20intermediary%20zone%2C%20which%20is%20functionally%20convergent%20with%20the%20protocorm%20of%20other%20plants%20with%20dust%20seeds%20such%20as%20orchids.%20Like%20the%20orchid%20protocorm%2C%20this%20intermediary%20zone%20produces%20a%20single%20meristem%3A%20however%2C%20unlike%20orchids%2C%20which%20produce%20a%20shoot%20meristem%2C%20pyroloids%20first%20generate%20a%20root%20meristem.%22%2C%22date%22%3A%222019%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Faob%5C%2Fmcy195%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220305-7364%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22Z57M64S6%22%5D%2C%22dateModified%22%3A%222025-03-07T09%3A33%3A27Z%22%7D%7D%5D%7D
1.
Remias, D.; Prochazkova, L.; Nedbalova, L.; Andersen, R. A. Two New Kremastochrysopsis Species, K. Austriaca Sp. Nov. and K. Americana Sp. Nov. (Chrysophyceae)1. J. Phycol. 2020, 56 (1), 135–145. https://doi.org/10.1111/jpy.12937.
1.
Kaderka, R.; Bulantová, J.; Heneberg, P.; Řezáč, M. Urticating Setae of Tarantulas (Araneae: Theraphosidae): Morphology, Revision of Typology and Terminology and Implications for Taxonomy. PLOS ONE 2019, 14 (11), e0224384. https://doi.org/10.1371/journal.pone.0224384.
1.
Oulehlová, D.; Kollárová, E.; Cifrová, P.; Pejchar, P.; Žárský, V.; Cvrčková, F. Arabidopsis Class I Formin FH1 Relocates between Membrane Compartments during Root Cell Ontogeny and Associates with Plasmodesmata. Plant Cell Physiol 2019, 60 (8), 1855–1870. https://doi.org/10.1093/pcp/pcz102.
1.
Lukes, J.; Hylis, M. In Love with Microsporidia for 60+Years: Jiri Vavra Passed Away IN MEMORIAM. J. Eukaryot. Microbiol. 2019, 66 (3), 533–534. https://doi.org/10.1111/jeu.12721.
1.
Nemcova, Y.; Kapustin, D. Mallomonas Teres, Sp. Nov. (Chrysophyceae), Simultaneously Revealed in Two Distant European Peat-Bog Regions. Cryptogam. Algol. 2019, 40 (4), 35–39. https://doi.org/10.5252/cryptogamie-algologe2019v40a4.
1.
Palma-Onetto, V.; Pflegerova, J.; Plarre, R.; Synek, J.; Cvacka, J.; Sillam-Dusses, D.; Sobotnik, J. The Labral Gland in Termites: Evolution and Function. Biol. J. Linnean Soc. 2019, 126 (3), 587–597. https://doi.org/10.1093/biolinnean/bly212.
1.
Leontovyč, R.; Young, N. D.; Korhonen, P. K.; Hall, R. S.; Bulantová, J.; Jeřábková, V.; Kašný, M.; Gasser, R. B.; Horák, P. Molecular Evidence for Distinct Modes of Nutrient Acquisition between Visceral and Neurotropic Schistosomes of Birds. Sci Rep 2019, 9 (1), 1347. https://doi.org/10.1038/s41598-018-37669-2.
1.
Musilova, Z.; Indermaur, A.; Bitja-Nyom, A. R.; Omelchenko, D.; Kłodawska, M.; Albergati, L.; Remišová, K.; Salzburger, W. Evolution of the Visual Sensory System in Cichlid Fishes from Crater Lake Barombi Mbo in Cameroon. Molecular Ecology 2019, 28 (23), 5010–5031. https://doi.org/10.1111/mec.15217.
1.
Kohutova, J.; Elsnicova, B.; Holzerova, K.; Neckar, J.; Sebesta, O.; Jezkova, J.; Vecka, M.; Vebr, P.; Hornikova, D.; Bacova, B. S.; Benova, T. E.; Hlavackova, M.; Tribulova, N.; Kolar, F.; Novakoval, O.; Zurmanova, J. M. Anti-Arrhythmic Cardiac Phenotype Elicited by Chronic Intermittent Hypoxia Is Associated With Alterations in Connexin-43 Expression, Phosphorylation, and Distribution. FRONTIERS IN ENDOCRINOLOGY 2019, 9. https://doi.org/10.3389/fendo.2018.00789.
1.
Kubátová, Z.; Pejchar, P.; Potocky, M.; Sekeres, J.; Zársky, V.; Kulich, I. Arabidopsis Trichome Contains Two Plasma Membrane Domains with Different Lipid Compositions Which Attract Distinct EXO70 Subunits. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2019, 20 (15). https://doi.org/10.3390/ijms20153803.
1.
Schwarzerová, K.; Bellinvia, E.; Martinek, J.; Sikorová, L.; Dostal, V.; Libusová, L.; Bokvaj, P.; Fischer, L.; Schmit, A. C.; Nick, P. Tubulin Is Actively Exported from the Nucleus through the Exportin1/CRM1 Pathway. SCIENTIFIC REPORTS 2019, 9. https://doi.org/10.1038/s41598-019-42056-6.
1.
Princová, J.; Schätz, M.; Tupa, O.; Prevorovsky, M. Analysis of Lipid Droplet Content in Fission and Budding Yeasts Using Automated Image Processing. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS 2019, No. 149. https://doi.org/10.3791/59889.
1.
Fawley, M. W.; Nemcova, Y.; Fawley, K. P. Phylogeny and Characterization of Paraeustigmatos Columelliferus, Gen. et Sp. Nov., a Member of the Eustigmatophyceae That May Represent a Basal Group within the Eustigmatales. FOTTEA 2019, 19 (2), 107–114. https://doi.org/10.5507/fot.2019.002.
1.
Stundlová, J.; Smíd, J.; Nguyen, P.; Stáhlavsky, F. Cryptic Diversity and Dynamic Chromosome Evolution in Alpine Scorpions (Euscorpiidae: Euscorpius). MOLECULAR PHYLOGENETICS AND EVOLUTION 2019, 134, 152–163. https://doi.org/10.1016/j.ympev.2019.02.002.
1.
Hernychova, L.; Rosulek, M.; Kádek, A.; Mareska, V.; Chmelík, J.; Adámková, L.; Grobárová, V.; Sebesta, O.; Kukacka, Z.; Skála, K.; Spiwok, V.; Cerny, J.; Novák, P. The C-Type Lectin-like Receptor Nkrp1b: Structural Proteomics Reveals Features Affecting Protein Conformation and Interactions. JOURNAL OF PROTEOMICS 2019, 196, 162–172. https://doi.org/10.1016/j.jprot.2018.11.007.
1.
Frolikova, M.; Valaskova, E.; Cerny, J.; Lumeau, A.; Sebkova, N.; Palenikova, V.; Sanchez-Hernandez, N.; Pohlova, A.; Manaskova-Postlerova, P.; Dvorakova-Hortova, K. Addressing the Compartmentalization of Specific Integrin Heterodimers in Mouse Sperm. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2019, 20 (5). https://doi.org/10.3390/ijms20051004.
1.
Batelka, J.; Prokop, J.; Pohl, H.; Bai, M.; Zhang, W.; Beutel, R. G. Highly Specialized Cretaceous Beetle Parasitoids (Ripiphoridae) Identified with Optimized Visualization of Microstructures. SYSTEMATIC ENTOMOLOGY 2019, 44 (2), 396–407. https://doi.org/10.1111/syen.12331.
1.
Nedvedova, I.; Kolar, D.; Neckar, J.; Kalous, M.; Pravenec, M.; Silhavil, J.; Korenkova, W.; Kolar, F.; Zurmanova, J. M. Cardioprotective Regimen of Adaptation to Chronic Hypoxia Diversely Alters Myocardial Gene Expression in SHR and SHR-Mt(BN) Conplastic Rat Strains. FRONTIERS IN ENDOCRINOLOGY 2019, 9. https://doi.org/10.3389/fendo.2018.00809.
1.
Figura, T.; Tylová, E.; Soch, J.; Selosse, M. A.; Ponert, J. In Vitro Axenic Germination and Cultivation of Mixotrophic Pyroloideae (Ericaceae) and Their Post-Germination Ontogenetic Development. ANNALS OF BOTANY 2019, 123 (4), 625–639. https://doi.org/10.1093/aob/mcy195.
2018
5891878
SIJXXBIS
1
https://raw.githubusercontent.com/Schebique/vmcf-konfmi/refs/heads/main/vmcf-web-style.csl
50
date
desc
4983
https://web.natur.cuni.cz/sekce-bi/VMCF/wp-content/plugins/zotpress/
statussuccessupdateneededfalseinstancefalsemetarequest_last0request_next0used_cachetruedatakeyUVW3CWZ6libraryid5891878metacreatorSummaryHubkaetal.parsedDate2018-12numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHubkaV.BarrsV.DudovaZ.SklenarF.KubatovaA.MatsuzawaT.YaguchiT.HorieY.NovakovaA.FrisvadJ.C.TalbotJ.J.KolarikM.ltbgtUnravellingSpeciesBoundariesintheAspergillusViridinutansComplexSectionFumigatiOpportunisticHumanandAnimalPathogensCapableofInterspecificHybridizationltbgt.ltigtPersoonialtigtltbgt2018ltbgtltigt41ltigt142x2013174.ltaclass039zp-DOIURL039href039httpsdoi.org10.3767persoonia.2018.41.08039gthttpsdoi.org10.3767persoonia.2018.41.08ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleUnravellingspeciesboundariesintheAspergillusviridinutanscomplexsectionFumigatiopportunistichumanandanimalpathogenscapableofinterspecifichybridizationcreatorscreatorTypeauthorfirstNameV.lastNameHubkacreatorTypeauthorfirstNameV.lastNameBarrscreatorTypeauthorfirstNameZ.lastNameDudovacreatorTypeauthorfirstNameF.lastNameSklenarcreatorTypeauthorfirstNameA.lastNameKubatovacreatorTypeauthorfirstNameT.lastNameMatsuzawacreatorTypeauthorfirstNameT.lastNameYaguchicreatorTypeauthorfirstNameY.lastNameHoriecreatorTypeauthorfirstNameA.lastNameNovakovacreatorTypeauthorfirstNameJ.C.lastNameFrisvadcreatorTypeauthorfirstNameJ.J.lastNameTalbotcreatorTypeauthorfirstNameM.lastNameKolarikabstractNoteAlthoughAspergiliusfumigatusisthemajoragentofinvasiveaspergillosisanincreasingnumberofinfectionsarecausedbyitscrypticspeciesespeciallyA.lentulusandtheA.viridinutansspeciescomplexAVSC.Theiridentificationisclinicallyrelevantbecauseofantifungaldrugresistanceandrefractoryinfections.SpeciesboundariesintheAVSCareunresolvedsincemostspecieshaveuniformmorphologyandproduceinterspecifichybridsinvitro.Clinicalandenvironmentalstrainsfromsixcontinentsn110werecharacterizedbyDNAsequencingoffourtosixloci.Biologicalcompatibilitiesweretestedwithinandbetweenmajorphylogeneticcladesandascosporemorphologywascharacterised.SpeciesdelimitationmethodsbasedonthemultispeciescoalescentmodelMSCsupportedrecognitionoftenspeciesincludingonenewspecies.FourspeciesareconfirmedopportunisticpathogensA.udagawaefollowedbyA.felisandA.pseudoviridinutansareknownfromopportunistichumaninfectionswhileA.felisfollowedbyA.udagawaeandA.wyomingensisareagentsoffelinesino-orbitalaspergillosis.Recentlydescribedhuman-pathogenicspeciesA.parafelisandA.pseudofelisaresynonymizedwithA.felisandanepitypeisdesignatedforA.udagawae.Intraspecificmatingassayshowedthatonlyafewoftheheterothallicspeciescanreadilygeneratesexualmorphsinvitro.Interspecificmatingassaysrevealedthatfivedifferentspeciescombinationswerebiologicallycompatible.Hybridascosporeshadatypicalsurfaceornamentationandsignificantlydifferentdimensionscomparedtoparentalspecies.ThissuggeststhatspecieslimitsintheAVSCaremaintainedbybothpre-andpost-zygoticbarriersandthesespeciesdisplayagreatpotentialforrapidadaptationandmodulationofvirulence.Thisstudyhighlightsthatasufficientnumberofstrainsrepresentinggeneticdiversitywithinaspeciesisessentialformeaningfulspeciesboundariesdelimitationincrypticspeciescomplexes.MSC-baseddelimitationmethodsarerobustandsuitabletoolsforevaluationofboundariesbetweenthesespecies.dateDEC2018sectionpartNumberpartTitleDOI10.3767persoonia.2018.41.08citationKeyurlPMIDPMCIDISSN0031-5850languageEnglishcollectionsSIJXXBISdateModified2025-11-07T092718ZkeyAEGERVP2libraryid5891878metacreatorSummaryVavraetal.parsedDate2018-11numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtVavraJ.FialaI.KrylovaP.PetrusekA.HylisM.ltbgtMolecularandStructuralAssessmentofMicrosporidiaInfectingDaphnidsThex201CObtusa-likex201DMicrosporidiaaBranchoftheMonophyleticAgglomeratidaeGladewiththeEstablishmentofaNewGenusConglomerataltbgt.ltigtJ.Invertebr.Pathol.ltigtltbgt2018ltbgtltigt159ltigt95x2013104.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.jip.2018.10.003039gthttpsdoi.org10.1016j.jip.2018.10.003ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMolecularandstructuralassessmentofmicrosporidiainfectingdaphnidsTheobtusa-likemicrosporidiaabranchofthemonophyleticAgglomeratidaeGladewiththeestablishmentofanewgenusConglomeratacreatorscreatorTypeauthorfirstNameJirilastNameVavracreatorTypeauthorfirstNameIvanlastNameFialacreatorTypeauthorfirstNamePavialastNameKrylovacreatorTypeauthorfirstNameAdamlastNamePetrusekcreatorTypeauthorfirstNameMiroslavlastNameHylisabstractNoteMicrosporidiaOpisthosporidiaMicrosporidiaarefrequentparasitesofplanktoniccladoceransincludingDaphniaCrustaceaBranchiopoda.AnalysisofavailablemoleculardataITSregionandpartialssuandlsurDNAoftheseparasitesindicatesthatmanymicrosporidiainfectingdaphnidshaveacommonancestorandrepresentalargeGladewhichsplitsduringevolutionintoanumberofwellsupportedsubclades.ThesesubcladesarecytologicallydifferentbutmaybemostconvenientlycharacterisedbytheirspecificITSbarcode.Wehaveanalysedoneofthesesubcladesandwedescribeanewmicrosporidiangenusandspeciescombinationandassemblealargegroupofstructurallyindistinguishablemicrosporidianparasitesthatinfectadiposecellsoftheirhostsandformpyriformsporesofacertaintypequotobtusesporesquot.Obtusesporesarenon-infectiousbyfeedingtotheircrustaceanhostsanditisplausiblethatmicrosporidiaformingthemactuallyareparasitesofinsectswithaquaticlarvalstageswithanobligatetwo-hostlifecycleanalogoustotheAmblyosporalifecycleinvolvingcopepodsandmosquitoes.dateNOV2018sectionpartNumberpartTitleDOI10.1016j.jip.2018.10.003citationKeyurlPMIDPMCIDISSN0022-20111096-0805languageEnglishcollectionsSIJXXBISdateModified2025-11-07T092718ZkeyUWXZREZQlibraryid5891878metacreatorSummarySahietal.parsedDate2018-10-05numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtSahiV.P.CifrovaP.Garcia-GonzalezJ.BabyI.K.MouilleG.GineauE.MuellerK.BaluskaF.SoukupA.PetrasekJ.SchwarzerovaK.ltbgtltigtArabidopsisThalianaltigtPlantsLackingtheARP23ComplexShowDefectsinCellWallAssemblyandAuxinDistributionltbgt.ltigtAnn.Bot.ltigtltbgt2018ltbgtltigt122ltigt5777x2013789.ltaclass039zp-DOIURL039href039httpsdoi.org10.1093aobmcx178039gthttpsdoi.org10.1093aobmcx178ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleiArabidopsisthalianaiplantslackingtheARP23complexshowdefectsincellwallassemblyandauxindistributioncreatorscreatorTypeauthorfirstNameVaiduryaPrataplastNameSahicreatorTypeauthorfirstNamePetralastNameCifrovacreatorTypeauthorfirstNameJudithlastNameGarcia-GonzalezcreatorTypeauthorfirstNameInnuKotannallastNameBabycreatorTypeauthorfirstNameGregorylastNameMouillecreatorTypeauthorfirstNameEmilielastNameGineaucreatorTypeauthorfirstNameKarellastNameMuellercreatorTypeauthorfirstNameFrantiseklastNameBaluskacreatorTypeauthorfirstNameAleslastNameSoukupcreatorTypeauthorfirstNameJanlastNamePetrasekcreatorTypeauthorfirstNameKaterinalastNameSchwarzerovaabstractNoteBackgroundandAimThecytoskeletonplaysanimportantroleinthesynthesisofplantcellwalls.Bothmicrotubulesandactincytoskeletonareknowntobeinvolvedinthemorphogenesisofplantcellsthroughtheirroleincellwallbuilding.TheroleofARP23-nucleatedactincytoskeletoninthemorphogenesisofcotyledonpavementcellshasbeendescribedbefore.SeedlingsofArabidopsismutantslackingafunctionalARP23complexdisplayspecificcellwall-associateddefects.MethodsInthreeindependentArabidopsismutantlineslackingsubunitsoftheARP23complex.phenotypesassociatedwiththelossofthecomplexwereanalysedthroughoutplantdevelopment.Organsizeandanatomycellwallcomposition.andauxindistributionwereinvestigated.KeyResultsARP23-relatedphenotypeisassociatedwithchangesincellwallcompositionandthephenotypeismanifestedespeciallyinmaturetissues.Cellwallsofmatureplantscontainlesscelluloseandahigheramountofhomogalacturonananddisplaychangesincellwalllignification.VascularbundlesofmutantinflorescencestemsshowachangedpatternofAUX1-YFPexpression.PlantslackingafunctionalARP23complexhavedecreasedbasipetalauxintransport.ConclusionsTheresultssuggestthattheARP23complexhasamorphogeneticfunctionrelatedtocellwallsynthesisandauxintransport.dateOCT52018sectionpartNumberpartTitleDOI10.1093aobmcx178citationKeyurlPMIDPMCIDISSN0305-73641095-8290languageEnglishcollectionsSIJXXBISdateModified2025-03-07T093324ZkeySZ4H49LElibraryid5891878metacreatorSummaryRotterovaetal.parsedDate2018-07numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtRotterovaJ.BourlandW.CepickaI.ltbgtTropidoatractidaeFam.Nov.aDeepBranchingLineageofMetopidaArmophoreaCiliophoraFoundinDiverseHabitatsandPossessingProkaryoticSymbiontsltbgt.ltigtProtistltigtltbgt2018ltbgtltigt169ltigt3362x2013405.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.protis.2018.04.003039gthttpsdoi.org10.1016j.protis.2018.04.003ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTropidoatractidaefam.nov.aDeepBranchingLineageofMetopidaArmophoreaCiliophoraFoundinDiverseHabitatsandPossessingProkaryoticSymbiontscreatorscreatorTypeauthorfirstNameJohanalastNameRotterovacreatorTypeauthorfirstNameWilliamlastNameBourlandcreatorTypeauthorfirstNameIvanlastNameCepickaabstractNoteWereportadiscoveryofanovelfamilyofanaerobicciliatesTropidoatractidaefam.nov.Phylogeneticanalysesbasedonthe18SrRNAgeneshowthatthefamilyTropidoatractidaecorrespondstothepreviouslyreportedGladeofenvironmentalsequencescloselyrelatedtothelineageconsistingofordersMetopidaandClevelandellida.ThefamilycomprisestwogeneraTropidoatractusandPalmarellaandfivespeciestwoofwhicharenewlydescribedherein.TropidoatractidaearecosmopolitanMetopidawithsparsesomaticandoralciliaturedeepcup-likebuccalcavityandhyalinecortexwithinterkinetalridges.Moreoverallspeciesoccurintwomorphotypesslenderandstout.Theyinhabitmicrooxicoranoxicfreshwaterbrackishandmarinesedimentsandpossessanaerobicmitochondrion-relatedorganellesandvariousprokaryoticsymbionts.ThediscoveryofTropidoatractidaeprovidesvaluableinformationabouttheevolutionofArmophoreaandgivesusinsightstothediversityandecologicalpreferencesofanaerobicciliatesingeneral.C2018ElsevierGmbH.Allrightsreserved.dateJUL2018sectionpartNumberpartTitleDOI10.1016j.protis.2018.04.003citationKeyurlPMIDPMCIDISSN1434-4610languageEnglishcollectionsSIJXXBISdateModified2025-11-07T092718Zkey2EPMIGW8libraryid5891878metacreatorSummaryVilimovaetal.parsedDate2018-05numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtVilimovaJ.KrizkovaP.JanstaP.McPhersonJ.E.ltbgtSexualDimorphisminExternalScentEfferentSystemofMetathoracicScentGlandsinAradidaeFirstEvidencewithinHeteropteraltbgt.ltigtZool.Anz.ltigtltbgt2018ltbgtltigt274ltigt115x2013122.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.jcz.2018.02.001039gthttpsdoi.org10.1016j.jcz.2018.02.001ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleSexualdimorphisminexternalscentefferentsystemofmetathoracicscentglandsinAradidaeFirstevidencewithinHeteropteracreatorscreatorTypeauthorfirstNameJitkalastNameVilimovacreatorTypeauthorfirstNamePetralastNameKrizkovacreatorTypeauthorfirstNamePetrlastNameJanstacreatorTypeauthorfirstNameJ.E.lastNameMcPhersonabstractNoteThemorphologyoftheexternalscentefferentsystemofthemetathoracicscentglandwasstudiedindetailinthreeAradusspeciesA.betulaeLinnaeus1758A.cinnamomeusPanzer1806andA.ribautiWagner1956HeteropteraAradidaeusingscanningelectronmicroscopy.Theostioleisslit-shapedandpoorlydevelopedtheperitremeisnotpresentandtheevaporatoriumconsistsofridgesandgroovesthataredirectedradiallyfromtheostiole.ThisinconspicuousexternalscentefferentsystemcouldrepresentanancestralconditionintheAradidae.Themicrosculptureofthecuticleoftheevaporatoriumishighlyspecies-specific.InA.betulaetheridgesandgroovesaremoredistinctinmalesthaninfemalesthemicrosculpturepatternoftheevaporatoriumismorehighlydevelopedinmalesthanfemalesandthemostdistinctsculptureispresentontheostiolemargininfemalesinA.cinnamomeustherearefewerdistinctdifferencesbetweenmalesandfemalesinthesizesoftheridgesandshapeoftheostiolemarginandthemicrosculpturepatternoftheevaporatoriumisidenticalinbothsexesandinA.ribautithesizeoftheridgesandgroovesareidenticalinbothsexesandthereareonlysmalldifferencesinthemicrosculpturepattern.ThesexualdimorphismintheexternalscentefferentsystemofthemetathoracicscentglandsinthesethreespeciesrepresentsthefirstreportofthisdimorphismintheHeteroptera.C2018ElsevierGmbH.Allrightsreserved.dateMAY2018sectionpartNumberpartTitleDOI10.1016j.jcz.2018.02.001citationKeyurlPMIDPMCIDISSN0044-5231languageEnglishcollectionsSIJXXBISdateModified2025-11-07T092718ZkeyA3CXUEERlibraryid5891878metacreatorSummaryAgarwaletal.parsedDate2018-03-21numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtAgarwalK.MachxE1x148R.PrasadD.K.ltbgtNon-HeuristicAutomaticTechniquesforOvercomingLowSignal-to-Noise-RatioBiasofLocalizationMicroscopyandMultipleSignalClassificationAlgorithmltbgt.ltigtSciRepltigtltbgt2018ltbgtltigt8ltigt14988.ltaclass039zp-DOIURL039href039httpsdoi.org10.1038s41598-018-23374-7039gthttpsdoi.org10.1038s41598-018-23374-7ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleNon-heuristicautomatictechniquesforovercominglowsignal-to-noise-ratiobiasoflocalizationmicroscopyandmultiplesignalclassificationalgorithmcreatorscreatorTypeauthorfirstNameKrishnalastNameAgarwalcreatorTypeauthorfirstNameRadeklastNameMachu00e1u0148creatorTypeauthorfirstNameDilipK.lastNamePrasadabstractNoteLocalizationmicroscopyandmultiplesignalclassificationalgorithmusetemporalstackofimageframesofsparseemissionsfromfluorophorestoprovidesuper-resolutionimages.Localizationmicroscopylocalizesemissionsineachimageindependentlyandlatercollatesthelocalizationsinalltheframesgivingsameweighttoeachframeirrespectiveofitssignal-to-noiseratio.Thisresultsinabiastowardsframeswithlowsignal-to-noiseratioandcausesclutteredbackgroundinthesuper-resolvedimage.User-definedheuristiccomputationalfiltersareemployedtoremoveasetoflocalizationsinanattempttoovercomethisbias.Multiplesignalclassificationperformseigen-decompositionoftheentirestackirrespectiveoftherelativesignal-to-noiseratiosoftheframesandusesathresholdtoclassifyeigenimagesintosignalandnullsubspaces.Thisresultsinunder-representationofframeswithlowsignal-to-noiseratiointhesignalspaceandover-representationinthenullspace.Thusmultiplesignalclassificationalgorithmsisbiasedagainstframeswithlowsignal-to-noiseratioresultingintosuppressionofthecorrespondingfluorophores.Thispaperpresentstechniquestoautomaticallydebiaslocalizationmicroscopyandmultiplesignalclassificationalgorithmofthesebiaseswithoutcompromisingtheirresolutionandwithoutemployingheuristicsuser-definedcriteria.Theeffectofdebiasingisdemonstratedthroughfivedatasetsofinvitroandfixedcellsamples.date2018-03-21sectionpartNumberpartTitleDOI10.1038s41598-018-23374-7citationKeyurlhttpswww.nature.comarticless41598-018-23374-7PMIDPMCIDISSN2045-2322languageencollectionsSIJXXBISdateModified2025-09-06T091229ZkeyZAJZU56Hlibraryid5891878metacreatorSummaryKlimaetal.parsedDate2018numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKlimaP.LankovaM.VandenbusscheF.StraetenD.V.D.PetrasekJ.ltbgtSilverIonsIncreasePlasmaMembranePermeabilitythroughModulationofIntracellularCalciumLevelsinTobaccoBY-2Cellsltbgt.ltigtPLANTCELLREPORTSltigtltbgt2018ltbgtltigt37ltigt5809x2013818.ltaclass039zp-DOIURL039href039httpsdoi.org10.1007s00299-018-2269-6039gthttpsdoi.org10.1007s00299-018-2269-6ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleSilverionsincreaseplasmamembranepermeabilitythroughmodulationofintracellularcalciumlevelsintobaccoBY-2cellscreatorscreatorTypeauthorfirstNamePetrlastNameKlimacreatorTypeauthorfirstNameMartinalastNameLankovacreatorTypeauthorfirstNameFiliplastNameVandenbusschecreatorTypeauthorfirstNameDominiqueVanDerlastNameStraetencreatorTypeauthorfirstNameJanlastNamePetrasekabstractNoteSilverionsincreaseplasmamembranepermeabilityforwaterandsmallorganiccompoundsthroughtheirstimulatoryeffectonplasmamembranecalciumchannelswithsubsequentmodulationofintracellularcalciumlevelsandionhomeostasis.Theactionofsilverionsattheplantplasmamembraneislargelyconnectedwiththeinhibitionofethylenesignallingthankstotheabilityofsilveriontoreplacethecoppercofactorintheethylenereceptor.Alinkcouplingtheactionofsilverionsandcellularauxineffluxhasbeensuggestedearlierbytheirpossibledirectinteractionwithauxineffluxcarriersorbyinfluencingplasmamembranepermeability.UsingtobaccoBY-2cellswedemonstrateherethatbesidesadramaticincreaseofeffluxofsyntheticauxins24-dichlorophenoxyaceticacid24-Dand1-naphthaleneaceticacidNAAtreatmentwithAgNO3resultedinenhancedeffluxofthecytokinintrans-zeatintZaswellastheauxinstructuralanaloguestryptophanTrpandbenzoicacidBA.TheapplicationofAgNO3wasaccompaniedbygradualwaterlossandplasmolysis.TheobservedeffectsweredependentontheavailabilityofextracellularcalciumionsCa2asshownbycomparisonoftransportassaysinCa2-richandCa2-freebuffersandupontreatmentwithinhibitorsofplasmamembraneCa2-permeablechannelsAl3andrutheniumredbothabolishingtheeffectofAgNO3.ConfocalmicroscopyofCa2-sensitivefluorescenceindicatorFluo-4FFacetoxymethylAMestersuggestedthattheextracellularCa2availabilityisnecessarytotriggertheresponsetosilverionsandthattheintracellularCa2poolaloneisnotsufficientforthiseffect.AltogetherourdatasuggestthatinplantcellstheeffectsofsilverionsoriginatefromtheprimalmodificationoftheinternalcalciumlevelspossiblybytheirinteractionwithCa2-permeablechannelsattheplasmamembrane.date2018sectionpartNumberpartTitleDOI10.1007s00299-018-2269-6citationKeyurlPMIDPMCIDISSN0721-7714languagecollectionsSIJXXBISdateModified2025-03-19T115834Zkey2FCYQJWZlibraryid5891878metacreatorSummaryAngelinietal.parsedDate2018numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtAngeliniJ.VosolsobeS.SkupaP.HoA.Y.Y.BellinviaE.ValentovaO.MarcJ.ltbgtPhospholipaseDDeltaAssiststoCorticalMicrotubuleRecoveryafterSaltStressltbgt.ltigtPROTOPLASMAltigtltbgt2018ltbgtltigt255ltigt41195x20131204.ltaclass039zp-DOIURL039href039httpsdoi.org10.1007s00709-018-1204-6039gthttpsdoi.org10.1007s00709-018-1204-6ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitlePhospholipaseDdeltaassiststocorticalmicrotubulerecoveryaftersaltstresscreatorscreatorTypeauthorfirstNameJindriskalastNameAngelinicreatorTypeauthorfirstNameStanislavlastNameVosolsobecreatorTypeauthorfirstNamePetrlastNameSkupacreatorTypeauthorfirstNameAngelaYeuanYenlastNameHocreatorTypeauthorfirstNameEricalastNameBellinviacreatorTypeauthorfirstNameOlgalastNameValentovacreatorTypeauthorfirstNameJanlastNameMarcabstractNoteThedynamicmicrotubulecytoskeletonplaysfundamentalrolesinthegrowthanddevelopmentofplantsincludingregulationoftheirresponsestoenvironmentalstress.Plantsexposedtohyper-osmoticstresscommonlyacclimateacquiringtolerancetovariablestresslevels.Theunderlyingcellularmechanismsarelargelyunknown.HereweshowforthefirsttimebyinvivoimagingapproachthatlinearpatternsofphospholipaseDdeltamatchthelocalizationofmicrotubulesinvariousbiologicalsystemsvalidatingpreviouslypredictedconnectionbetweenphospholipaseDdeltaandmicrotubules.BoththemicrotubuleandlinearphospholipaseDdeltastructuresweredisintegratedinafewminutesaftertreatmentwithoryzalinorsalt.MoreoverbyusingimmunofluorescenceconfocalmicroscopyofthecellsintherootelongationzoneofArabidopsiswehaveshownthatthecorticalmicrotubulesrapidlydepolymerizedwithin30minoftreatmentwith150or200mMNaCl.Within5hoftreatmentthedensityofmicrotubulearrayswaspartiallyrestored.AT-DNAinsertionalmutantlackingphospholipaseDdeltashowedpoorrecoveryofmicrotubulearraysfollowingsaltexposition.TherestorationofmicrotubuleswassignificantlyretardedaswellastherateofrootgrowthbutrootsofoverexpressorGFP-PLDdeltapreparedinourlabhavegrownslightlybettercomparedtowild-typeplants.OurresultsindicatethatphospholipaseDdeltaisinvolvedinsaltstresstolerancepossiblybydirectanchoringandstabilizationofdenovoemergingmicrotubulestotheplasmamembraneprovidingnovelinsightintocommonmolecularmechanismduringvariousstressevents.date2018sectionpartNumberpartTitleDOI10.1007s00709-018-1204-6citationKeyurlPMIDPMCIDISSN0033-183XlanguagecollectionsSIJXXBISdateModified2025-03-19T115834ZkeyFZZBPM9Hlibraryid5891878metacreatorSummarySvobodovaetal.parsedDate2018numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtSvobodovaI.BhattaracharyaA.IveticM.BendovaZ.ZemkovaH.ltbgtCircadianATPReleaseinOrganotypicCulturesoftheRatSuprachiasmaticNucleusIsDependentonP2X7andP2YReceptorsltbgt.ltigtFRONTIERSINPHARMACOLOGYltigtltbgt2018ltbgtltigt9ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.3389fphar.2018.00192039gthttpsdoi.org10.3389fphar.2018.00192ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleCircadianATPReleaseinOrganotypicCulturesoftheRatSuprachiasmaticNucleusIsDependentonP2X7andP2YReceptorscreatorscreatorTypeauthorfirstNameI.lastNameSvobodovacreatorTypeauthorfirstNameA.lastNameBhattaracharyacreatorTypeauthorfirstNameM.lastNameIveticcreatorTypeauthorfirstNameZ.lastNameBendovacreatorTypeauthorfirstNameH.lastNameZemkovaabstractNoteThecircadianrhythmsinphysiologicalandbehavioralfunctionsaredrivenbyapacemakerlocatedinthesuprachiasmaticnucleusSCN.Therhythmscontinueinconstantdarknessanddependoncell-cellcommunicationbetweenneuronsandglia.TheSCNastrocytesgeneratealsoacircadianrhythminextracellularadenosine5039-triphosphateATPaccumulationbutmolecularmechanismsthatregulateATPreleasearepoorlyunderstood.HerewetestedthehypothesisthatATPisreleasedviatheplasmamembranepurinergicP2X7receptorsP2X7RsandP2YreceptorsP2YRswhichhavebeenpreviouslyshowntobeexpressedintheSCNtissueattranscriptionallevel.WehaveinvestigatedthishypothesisusingSCNorganotypicculturesprimaryculturesofSCNastrocytesATPbioluminescentassaysimmunohistochemistrypatch-clampingandcalciumimaging.WefoundthatextracellularATPaccumulationinorganotypicculturesfollowedacircadianrhythmwithapeakbetween2400and0400handthetroughatsimilarto1200h.ATPrhythmwasinhibitedbyapplicationofAZ10606120A438079andBBGspecificblockersofP2X7RandpotentiatedbyGW791343apositiveallostericmodulatorofthisreceptor.Double-immunohistochemicalstainingrevealedhighexpressionoftheP2X7RproteininastrocytesofSCNslices.PPADSanon-specificP2antagonistandMRS2179specificP2Y1RantagonistalsoabolishedATPrhythmwhereasthespecificP2X4Rblocker5-BDBDwasnoteffective.Thepannexin-1hemichannelblockercarbenoxolonedisplayedapartialinhibitoryeffect.TheP2Y1RagonistMRS2365andtheP2Y2RagonistMRS2768potentiatedATPreleaseinorganotypicculturesandincreaseintracellularCa2levelinculturedastrocytes.ThusSCNutilizesmultiplepurinergicreceptorsystemsandpannexin-1hemichannelstoreleaseATP.date2018sectionpartNumberpartTitleDOI10.3389fphar.2018.00192citationKeyurlPMIDPMCIDISSN1663-9812languagecollectionsSIJXXBISdateModified2025-03-19T115836ZkeyHJUJFJFVlibraryid5891878metacreatorSummaryMerweetal.parsedDate2018numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMerweI.vanderLukxE1tsA.BlxE1hovxE1V.OosthuizenM.K.BennettN.C.NemecP.ltbgtTheTopographyofRodsConesandIntrinsicallyPhotosensitiveRetinalGanglionCellsintheRetinasofaNocturnalltigtMicaelamysNamaquensisltigtandaDiurnalltigtRhabdomysPumilioltigtRodentltbgt.ltigtPLOSONEltigtltbgt2018ltbgtltigt13ltigt8.ltaclass039zp-DOIURL039href039httpsdoi.org10.1371journal.pone.0202106039gthttpsdoi.org10.1371journal.pone.0202106ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleThetopographyofrodsconesandintrinsicallyphotosensitiveretinalganglioncellsintheretinasofanocturnaliMicaelamysnamaquensisiandadiurnaliRhabdomyspumilioirodentcreatorscreatorTypeauthorfirstNameI.vanderlastNameMerwecreatorTypeauthorfirstNameA.lastNameLuku00e1tscreatorTypeauthorfirstNameV.lastNameBlu00e1hovu00e1creatorTypeauthorfirstNameM.K.lastNameOosthuizencreatorTypeauthorfirstNameN.C.lastNameBennettcreatorTypeauthorfirstNameP.lastNameNemecabstractNoteWeusedimmunocytochemistrytodeterminethepresenceandtopographicaldensitydistributionsofrodsconesandintrinsicallyphotosensitiveretinalganglioncellsipRGCsinthefour-stripedfieldmouseRhabdomyspumilioandtheNamaquarockmouseMicaelamysnamaquensis.Bothspeciespossessedduplexretinasthatwereroddominated.InR.pumiliothedensityofbothconesandrodswerehighconetorodratio11.23andreflectedthespecies039fundamentallydiurnalbutlargelycrepuscularlifestyle.SimilarlytheratioofconestorodsinM.namaquensis112.4reflecteditsnocturnallifestyle.SimilarroddensitypeakswereobservedR.pumiliosimilarto84467mm2M.namaquensissimilarto81088mm2butadensitygradientyieldedhighervaluesinthecentralsimilarto56618mm2ratherthanintheperipheralretinalregionsimilarto32689mm2inR.pumilio.TwoseparateconetypesS-conesandML-coneswereidentifiedimplyingdichromaticcolorvisioninthestudyspecies.InM.namaquensisbothconepopulationsshowedacentro-peripheraldensitygradientandaconsistentS-toML-coneratiosimilarto17.8.InR.pumilioSconesshowedacentro-peripheralgradientS-toML-coneratiocentral17.8peripheral16.8whichappearedtoformavisualstreakandaspecializedareaofML-conesS-toML-coneratio115wasobservedinferiortotheopticnerve.Thenumberofphotoreceptorsperlineardegreeofvisualangleestimatedfrompeakphotoreceptordensitiesandeyesizewerefourconesand15rodsperdegreeinM.namaquensisand11conesand12rodsperdegreeinR.pumilio.ThusinnocturnalM.namaquensisrodsprovidemuchfinerimagesamplingthanconeswhereasindiurnalcrepuscularR.pumiliobothphotoreceptortypesprovidefineimagesampling.IpRGCswerecomparablysparseinR.pumiliototal1012andM.namaquensistotal862butwerehomogeneouslydistributedinM.namaquensisanddensestinthedorso-nasalquadrantinR.pumilio.Theadaptivesignificanceofthelatterneedsfurtherinvestigation.date2018sectionpartNumberpartTitleDOI10.1371journal.pone.0202106citationKeyurlPMIDPMCIDISSN1932-6203languagecollectionsSIJXXBISdateModified2025-03-19T115836ZkeyG7LG6CXQlibraryid5891878metacreatorSummaryKulichetal.parsedDate2018numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKulichI.VojtxEDkovxE1Z.SabolP.OrtmannovxE1J.NedelaV.TihlarikovxE1E.ZxE1rskyV.ltbgtExocystSubunitEXO70H4HasaSpecificRoleinCalloseSynthaseSecretionandSilicaAccumulationltbgt.ltigtPLANTPHYSIOLOGYltigtltbgt2018ltbgtltigt176ltigt32040x20132051.ltaclass039zp-DOIURL039href039httpsdoi.org10.1104pp.17.01693039gthttpsdoi.org10.1104pp.17.01693ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleExocystSubunitEXO70H4HasaSpecificRoleinCalloseSynthaseSecretionandSilicaAccumulationcreatorscreatorTypeauthorfirstNameI.lastNameKulichcreatorTypeauthorfirstNameZ.lastNameVojtu00edkovu00e1creatorTypeauthorfirstNameP.lastNameSabolcreatorTypeauthorfirstNameJ.lastNameOrtmannovu00e1creatorTypeauthorfirstNameV.lastNameNedelacreatorTypeauthorfirstNameE.lastNameTihlarikovu00e1creatorTypeauthorfirstNameV.lastNameZu00e1rskyabstractNoteBiogenesisoftheplantsecondarycellwallinvolvesmanyimportantaspectssuchasphenoliccompounddepositionandoftensilicaencrustation.PreviouslywedemonstratedtheimportanceoftheexocystsubunitEXO70H4forbiogenesisofthetrichomesecondarycellwallnamelyfordepositionoftheautofluorescentandcallose-richcellwalllayer.HerewerevealthatEXO70H4drivencellwallbiogenesisisconstitutivelyactiveinthematuretrichomebutalsocanbeactivatedelsewhereuponpathogenattackgivingthisstudyabroadersignificancewithanoverlapintophytopathology.ToaddressthespecificityofEXO70H4amongtheEXO70familywecomplementedtheexo70H4-1mutantby18differentArabidopsisArabidopsisthalianaEXO70paralogssubclonedundertheEXO70H4promoter.OnlyEXO70H4hadthecapacitytorescuetheexo70H4-1trichomephenotype.Callosedepositionphenotypeofexo70H4-1mutantiscausedbyimpairedsecretionofPMR4acallosesynthaseresponsibleforthesynthesisofcalloseinthetrichome.PMR4colocalizeswithEXO70H4onplasmamembranemicrodomainsthatdonotdevelopintheexo70H4-1mutant.Usingenergy-dispersivex-raymicroanalysisweshowthatbothEXO70H4-andPMR4-dependentcallosedepositioninthetrichomeareessentialforcellwallsilicification.date2018sectionpartNumberpartTitleDOI10.1104pp.17.01693citationKeyurlPMIDPMCIDISSN0032-0889languagecollectionsSIJXXBISdateModified2025-03-19T115836ZkeyZIE65TAZlibraryid5891878metacreatorSummaryPichrtovu00e1etal.parsedDate2018numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtPichrtovxE1M.HolzingerA.KulichovxE1J.RysxE1nekD.SoljakovxE1T.TrumhovxE1K.NemcovaY.ltbgtMolecularandMorphologicalDiversityofltigtZygnemaltigtandltigtZygnemopsisltigtZygnematophyceaeStreptophytafromSvalbardHighArcticltbgt.ltigtEUROPEANJOURNALOFPHYCOLOGYltigtltbgt2018ltbgtltigt53ltigt4492x2013508.ltaclass039zp-DOIURL039href039httpsdoi.org10.108009670262.2018.1476920039gthttpsdoi.org10.108009670262.2018.1476920ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMolecularandmorphologicaldiversityofiZygnemaiandiZygnemopsisiZygnematophyceaeStreptophytafromSvalbardHighArcticcreatorscreatorTypeauthorfirstNameM.lastNamePichrtovu00e1creatorTypeauthorfirstNameA.lastNameHolzingercreatorTypeauthorfirstNameJ.lastNameKulichovu00e1creatorTypeauthorfirstNameD.lastNameRysu00e1nekcreatorTypeauthorfirstNameT.lastNameSoljakovu00e1creatorTypeauthorfirstNameK.lastNameTrumhovu00e1creatorTypeauthorfirstNameY.lastNameNemcovaabstractNoteFilamentousconjugatinggreenmicroalgaeZygnematophyceaeStreptophytabelongtothemostcommonprimaryproducersinpolarhydro-terrestrialenvironmentssuchasmeltwaterstreamletsandshallowpools.ThematsformedbytheseorganismsaremostlycomposedofsterilefilamentswithZygnemamorphologybuttheextentoftheirdiversityremainsunknown.Traditionaltaxonomyofthisgroupisbasedonreproductivemorphologybutsexualreproductionconjugationandformationofresistantzygosporesisveryrareinextremeconditions.InthepresentstudywegavethefirstrecordofzygosporeformationinSvalbardfieldsamplesandidentifiedconjugatingfilamentsasZygnemopsislamellataandZygnemacf.calosporum.WeappliedmolecularphylogenytostudygeneticdiversityofsterileZygnemafilamentsfromSvalbardintheHighArctic.Basedonanalysisof143rbcLsequenceswerevealedasurprisinglyhighmoleculardiversity12ArcticZygnemagenotypesandoneZygnemopsisgenotypewerefound.InadditionwecharacterizedindividualArcticgenotypesbasedoncellwidthandchloroplastmorphologyusinglightandconfocallaserscanningmicroscopy.OurfindingshighlighttheimportanceofamolecularapproachwhenworkingwithsterilefilamentousZygnematophyceaeashiddendiversitymightbeverybeneficialforadaptationtoharshenvironmentalconditionsandexperimentalresultscouldbemisinterpretedwhenhiddendiversityisneglected.date2018sectionpartNumberpartTitleDOI10.108009670262.2018.1476920citationKeyurlPMIDPMCIDISSN0967-0262languagecollectionsSIJXXBISdateModified2025-03-19T115836ZkeyWQUFRGSAlibraryid5891878metacreatorSummaryStu00e1hlavskyetal.parsedDate2018numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtStxE1hlavskyF.OpatovaV.JustP.LotzL.N.HaddadC.R.ltbgtMolecularTechniqueRevealsHighVariabilityof18SrDNADistributioninHarvestmenOpilionesPhalangiidaefromSouthAfricaltbgt.ltigtCOMPARATIVECYTOGENETICSltigtltbgt2018ltbgtltigt12ltigt141x201359.ltaclass039zp-DOIURL039href039httpsdoi.org10.3897CompCytogen.v12i1.21744039gthttpsdoi.org10.3897CompCytogen.v12i1.21744ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMoleculartechniquerevealshighvariabilityof18SrDNAdistributioninharvestmenOpilionesPhalangiidaefromSouthAfricacreatorscreatorTypeauthorfirstNameF.lastNameStu00e1hlavskycreatorTypeauthorfirstNameV.lastNameOpatovacreatorTypeauthorfirstNameP.lastNameJustcreatorTypeauthorfirstNameL.N.lastNameLotzcreatorTypeauthorfirstNameC.R.lastNameHaddadabstractNoteTheknowledgeofcytogeneticsintheharvestmenfamilyPhalangiidaehasbeenbasedontaxafromtheNorthernHemisphere.WeperformedcytogeneticanalysisonGuruiaafricanaKarsch18782n24andfourspeciesofthegenusRhampsinitusSimon18792n242634fromSouthAfrica.Fluorescenceinsituhybridizationwithan18SrDNAprobewasusedtoanalyzethenumberandthedistributionofthisclusterinthefamilyPhalangiidaeforthefirsttime.Theresultssupportthecytogeneticcharacteristicstypicalforthemajorityofharvestmentaxai.e.thepredominanceofsmallbiarmedchromosomesandtheabsenceofmorphologicallywell-differentiatedsexchromosomesasanancestralstate.Weidentifiedthenumberof18SrDNAsitesrangingfromtwoinR.qachasnekiKauri1962toseveninonepopulationofR.leighiPocock1903.Moreoverwefounddifferencesinthenumberandlocalizationof18SrDNAsitesinR.leighibetweenpopulationsfromtwolocalitiesandbetweensexesofR.capensisLoman1898.Theheterozygousstatesofthe18SrDNAsitesinthesespeciesmayindicatethepresenceofXXXYandZZZWsexchromosomesandthepossibleexistenceofthesesystemsinharvestmenisdiscussed.Thevariabilityofthe18SrDNAsitesindicatesintensivechromosomalchangesduringthedifferentiationofthekaryotypeswhichisincontrasttotheusualuniformityinchromosomalmorphologyknownfromharvestmensofar.date2018sectionpartNumberpartTitleDOI10.3897CompCytogen.v12i1.21744citationKeyurlPMIDPMCIDISSN1993-0771languagecollectionsSIJXXBISdateModified2025-03-19T115835Zkey8JYTXK25libraryid5891878metacreatorSummaryStu00e1hlavskyetal.parsedDate2018numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtStxE1hlavskyF.StundlovxE1J.LoweG.StockmannM.KovarxEDkF.ltbgtApplicationofCytogeneticMarkersintheTaxonomyofFlatRockScorpionsScorpionesHormuridaewiththeDescriptionofltigtHadogenesWeygoldtiltigtSpn.ltbgtltigtZOOLOGISCHERANZEIGERltigtltbgt2018ltbgtltigt273ltigt173x2013182.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.jcz.2018.01.007039gthttpsdoi.org10.1016j.jcz.2018.01.007ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleApplicationofcytogeneticmarkersinthetaxonomyofflatrockscorpionsScorpionesHormuridaewiththedescriptionofiHadogenesweygoldtiispn.creatorscreatorTypeauthorfirstNameF.lastNameStu00e1hlavskycreatorTypeauthorfirstNameJ.lastNameStundlovu00e1creatorTypeauthorfirstNameG.lastNameLowecreatorTypeauthorfirstNameM.lastNameStockmanncreatorTypeauthorfirstNameF.lastNameKovaru00edkabstractNoteInthepresentstudyweperformedthefirstcomparativecytogeneticstudyinHadogenesspeciesusingbothstandardandmolecularcytogeneticapproaches.Informationaboutthediploidsetnumberanddistributionof18SrDNAandtelomericsequenceswasobtainedfromthreeSouthAfricanspeciesHadogenestrichiurusGervais1843H.zuluanusLawrence1937andH.weygoldtisp.n..AllspeciesanalyseddifferconsiderablyinthenumberofchromosomesH.trichiurus2n48H.zuluanus2n80H.weygoldtisp.n.2n113.Incontrastthenumberof18SrDNAclustersanddistributionoftelomericsequencesrepresentratherstablecytogeneticcharactersinHadogenes.Withinallkaryotypesweidentifiedonepairof18SrDNAclusters.Thetelomericsignalswereexclusivelyontheterminalchromosomalregions.Interestinglythechromosomallocationof18SrDNAclustersvariedfromterminaltointerstitialinspecieskaryotypesindicatingthepresenceofhiddenstructuralchromosomalchanges.AdditionallythepresentcomparativestudyiscomplementedbythedescriptionofanewspeciesH.weygoldtisp.n.basedonspecifickaryotypefeaturesandmorphologicalcharacters.FinallyourcytogeneticresultsarecomparedwithknownchromosomaldataofotherHadogenesspeciesandtheuseofcytogeneticapproachesinthetaxonomyofscorpionsisdiscussed.C2018ElsevierGmbH.Allrightsreserved.date2018sectionpartNumberpartTitleDOI10.1016j.jcz.2018.01.007citationKeyurlPMIDPMCIDISSN0044-5231languagecollectionsSIJXXBISdateModified2025-03-19T115835Zkey4DVVDTZFlibraryid5891878metacreatorSummaryHirmanetal.parsedDate2018numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHirmanM.KotykM.VaradinovxE1Z.K.StxE1hlavskyF.ltbgtFIRSTCYTOGENETICSTUDYOFAMEMBEROFTHEHARVESTMANFAMILYNEOGOVEIDAEOPILIONESCYPHOPHTHALMISTERNOPHTHALMIFROMCAMEROONWITHDESCRIPTIONOFANEWSPECIESltigtPAROGOVIAPARASIRONOIDESltigtSP.NOV.ltbgtltigtANNALESZOOLOGICIltigtltbgt2018ltbgtltigt68ltigt4867x2013877.ltaclass039zp-DOIURL039href039httpsdoi.org10.316100034541ANZ2018.68.4.010039gthttpsdoi.org10.316100034541ANZ2018.68.4.010ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleFIRSTCYTOGENETICSTUDYOFAMEMBEROFTHEHARVESTMANFAMILYNEOGOVEIDAEOPILIONESCYPHOPHTHALMISTERNOPHTHALMIFROMCAMEROONWITHDESCRIPTIONOFANEWSPECIESiPAROGOVIAPARASIRONOIDESiSP.NOV.creatorscreatorTypeauthorfirstNameM.lastNameHirmancreatorTypeauthorfirstNameM.lastNameKotykcreatorTypeauthorfirstNameZ.K.lastNameVaradinovu00e1creatorTypeauthorfirstNameF.lastNameStu00e1hlavskyabstractNoteTheharvestmanfamilyNeogoveidaesuborderCyphophthalmiisdistributedacrossNeotropicssouth-easternUSAandWesternandCentralAfrica.NeverthelessthecoreofitsknowndiversityliesinAmericas.ThesolegenusknownfromAfricaisParogoviawithonlythreespeciesdescribedsofar.HerewedescribeanewspeciesParogoviaparasironoidessp.nov.fromCameroonwhichismorphologicallyclosetoP.sironoides.Inadditiontotheanalysesofexternalmorphologyspermatopositorandovipositorwealsodescribedthekaryotype2n32acourseofthemalemitosisandmeiosisandthedistributionofmajorribosomalRNAgenesusingfluorescenceinsituhybridizationFISHwith18SrDNAprobe.Inthekaryotypetheone-armedchromosomespredominatedandwedetectedonepairofthe18SrDNAclusterclosetocentromereregiononlongarmsofsubtelocentricchromosomes.OurstudyrepresentsthefirstcytogeneticdataininfraorderSternophthalmiandplaysanimportantroleinthereconstructionofthekaryotypeevolutionofCyphophthalmi.date2018sectionpartNumberpartTitleDOI10.316100034541ANZ2018.68.4.010citationKeyurlPMIDPMCIDISSN0003-4541languagecollectionsSIJXXBISdateModified2025-03-19T115834ZkeyLH5X2FX9libraryid5891878metacreatorSummaryNedvedovaetal.parsedDate2018numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtNedvedovaI.KolarD.ElsnicovaB.HornikovaD.NovotnyJ.KalousM.PravenecM.NeckarJ.KolarF.ZurmanovaJ.M.ltbgtMitochondrialGenomeModulatesMyocardialAktGlutHKSalvagePathwayinSpontaneouslyHypertensiveRatsAdaptedtoChronicHypoxialtbgt.ltigtPHYSIOLOGICALGENOMICSltigtltbgt2018ltbgtltigt50ltigt7532x2013541.ltaclass039zp-DOIURL039href039httpsdoi.org10.1152physiolgenomics.00040.2017039gthttpsdoi.org10.1152physiolgenomics.00040.2017ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMitochondrialgenomemodulatesmyocardialAktGlutHKsalvagepathwayinspontaneouslyhypertensiveratsadaptedtochronichypoxiacreatorscreatorTypeauthorfirstNameIvetalastNameNedvedovacreatorTypeauthorfirstNameDavidlastNameKolarcreatorTypeauthorfirstNameBarbaralastNameElsnicovacreatorTypeauthorfirstNameDanielalastNameHornikovacreatorTypeauthorfirstNameJirilastNameNovotnycreatorTypeauthorfirstNameMartinlastNameKalouscreatorTypeauthorfirstNameMichallastNamePraveneccreatorTypeauthorfirstNameJanlastNameNeckarcreatorTypeauthorfirstNameFrantiseklastNameKolarcreatorTypeauthorfirstNameJitkaM.lastNameZurmanovaabstractNoteRecentlywehaveshownthatadaptationtocontinuousnormobarichypoxiaCNHdecreasesmyocardialischemiareperfusioninjuryinspontaneouslyhypertensiveratsSHRandinaconplasticstrainSHR-mtBN.TheprotectiveeffectwasstrongerinthelattergroupcharacterizedbyaselectivereplacementoftheSHRmitochondrialgenomewiththatofamoreischemia-resistantBrownNorwaystrain.TheaimofthepresentstudywastoexaminethepossibleinvolvementofthehypoxiainduciblefactorHIF-dependentpathwayoftheproteinkinaseBglucosetransportershexokinaseAktGLUTHKinthismitochondrialgenome-relateddifferenceofthecardioprotectivephenotype.Adultmaleratswereexposedfor3wktoCNHFIO20.1.TheexpressionofdominantisoformsofAkt.GunandHKinleftventricularmyocardiumwasdeterminedbyreal-timeRT-PCRandWesternblotting.SubcellularlocalizationofGLUTswasassessedbyquantitativeimmunofluorescence.WhereasadaptationtohypoxiamarkedlyupregulatedproteinexpressionofHK2GLUT1andGLUT4inbothratstrainsAkt2proteinlevelwassignificantlyincreasedinSHR-mtBNonly.Interestingly.ahighercontentofHK2wasrevealedinthesarcoplasmicreticulum-enrichedfractioninSHR-mtBNafterCNH.TheincreasedactivityofHKdeterminedinthemitochondrialfractionafterCNHinbothstrainssuggestedanincreaseofHKassociationwithmitochondria.InterestinglyHIF1amRNAincreasedandHIF2amRNAdecreasedafterCNHtheformereffectbeingmorepronouncedinSHR-mtBNthaninSHR.PleiotropiceffectsofupregulatedAkt2alongwithHKtranslocationtomitochondriaandmitochondria-associatedmembranescanpotentiallycontributetoastrongerCNH-affordedcardioprotectioninSHR-mtBNcomparedwithprogenitorSHR.date2018sectionpartNumberpartTitleDOI10.1152physiolgenomics.00040.2017citationKeyurlPMIDPMCIDISSN1094-8341languagecollectionsSIJXXBISdateModified2025-03-19T115834ZkeyFW3736J2libraryid5891878metacreatorSummarySladeketal.parsedDate2018numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtSladekV.SabolovaV.SebestaO.ZikmundT.KaiserJ.CerevkovaS.ltbgtEffectofDerivingPeriostealandEndostealContoursfrommicroCTScansonComputationofCross-SectionalPropertiesinNon-AdultsTheFemurltbgt.ltigtJOURNALOFANATOMYltigtltbgt2018ltbgtltigt233ltigt3381x2013393.ltaclass039zp-DOIURL039href039httpsdoi.org10.1111joa.12835039gthttpsdoi.org10.1111joa.12835ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleEffectofderivingperiostealandendostealcontoursfrommicroCTscansoncomputationofcross-sectionalpropertiesinnon-adultsthefemurcreatorscreatorTypeauthorfirstNameVladimirlastNameSladekcreatorTypeauthorfirstNameVeronikalastNameSabolovacreatorTypeauthorfirstNameOndrejlastNameSebestacreatorTypeauthorfirstNameTomaslastNameZikmundcreatorTypeauthorfirstNameJozeflastNameKaisercreatorTypeauthorfirstNameSimonalastNameCerevkovaabstractNoteDerivationofperiostealandendostealcontourstakenfromtransversallongbonecross-sectionslimitstheaccuracyofcalculatedbiomechanicalproperties.Althoughseveraltechniquesareavailableforderivingbothcontourstheeffectofthesetechniquesonaccuracyofcalculatedcross-sectionalpropertiesinnon-adultsisunknown.Weexamineasampleof86non-adultfemorafrombirthto12yearsofagetoestimatetheeffectoferrorinderivingperiostealandendostealcontoursoncross-sectionalproperties.Midshaftcross-sectionsweretakenfrommicroCTscansandcontourswerederivedusingmanualfullyautomaticsplineandellipsetechniques.AgreementbetweentechniqueswasassessedagainstmanuallytracedperiostealandendostealcontoursusingpercentpredictionerrorPEreducedmajoraxisanalysisandlimitsofagreement.ThePEswerehighestinthemedullaryareaandlowestinthetotalarea.MeanPEsweresufficientlybelowthe5levelofacceptableerrorexceptformedullaryareasbutindividualvaluescangreatlyexceedthis5boundarygiventhehighstandarddeviationofPEmeansandwideminimum-maximumrangeofPEs.Automaticprocessingproducesgreatererrorsthandoescombinationwithmanualsplineandellipseprocessing.Althoughperiostealcontourisestimatedwithstrongeragreementcomparedwithendostealcontourerrorinderivingperiostealcontourhasasubstantiallygreatereffectoncalculatedsectionmodulithandoeserrorinderivingendostealcontours.Weobservednosizeeffectontheresultingbias.Neverthelesscross-sectionalpropertiesinayoungeragecategorymaybeestimatedwithgreatererrorcomparedwithinanolderagecategory.Weconcludethatnon-adultmidshaftcross-sectionalpropertiescanbederivedfrommicroCTscansoffemoraldiaphyseswithmeanerroroflt5andthatderivationofendostealcontourcanbesimplifiedbytheellipsetechniquebecausefullyautomaticderivationofendostealcontourmayincreasetheresultingerrorespeciallyinsmallsamples.date2018sectionpartNumberpartTitleDOI10.1111joa.12835citationKeyurlPMIDPMCIDISSN0021-8782languagecollectionsSIJXXBISdateModified2025-03-19T115833ZkeyLXGGVCUBlibraryid5891878metacreatorSummaryPohletal.parsedDate2018numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtPohlH.BatelkaJ.ProkopJ.MuellerP.YavorskayaM.I.BeutelR.G.ltbgtANeedleinaHaystackMesozoicOriginofParasitisminStrepsipteraRevealedbyFirstDefiniteCretaceousPrimaryLarvaInsectaltbgt.ltigtPEERJltigtltbgt2018ltbgtltigt6ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.7717peerj.5943039gthttpsdoi.org10.7717peerj.5943ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleAneedleinahaystackMesozoicoriginofparasitisminStrepsipterarevealedbyfirstdefiniteCretaceousprimarylarvaInsectacreatorscreatorTypeauthorfirstNameHanslastNamePohlcreatorTypeauthorfirstNameJanlastNameBatelkacreatorTypeauthorfirstNameJakublastNameProkopcreatorTypeauthorfirstNamePatricklastNameMuellercreatorTypeauthorfirstNameMargaritaI.lastNameYavorskayacreatorTypeauthorfirstNameRolfG.lastNameBeutelabstractNoteTwistedwingedinsectsStrepsipteraareahighlyspecializedsmallorderofparasiticinsects.Whetherparasitismdevelopedatanearlyorlatestageintheevolutionofthegroupwasunknown.HerewerecordanddescribethefirstdefiniteMesozoicstrepsipteranprimarylarvaembeddedinBurmeseambersimilarto99millionyearsago.Thisextendstheoriginofparasitismbackbyatleastsimilarto50millionyearsandrevealsthatthisspecializedlifestylehasevolvedintheMesozoicorevenearlierinthegroup.TheextremelysmallfirstinstardisplaysalldiagnosticcharactersofstrepsipteranimmaturesofthisstageandisnearlyidenticalwiththoseofMengenillidaeoneofthemostu201cancestralu201dextantstrepsipterantaxa.Thisdemonstratesaremarkableevolutionarystasisover100millionyears.ThenewfindingstronglyweakensthecaseofsmalllarvaeembeddedinCretaceousamberinterpretedasstrepsipteranimmatures.Theydifferinmanystructuralfeaturesfromextantstrepsipteranprimarylarvaeandareverylikelyparasiticbeetlelarvae.date2018sectionpartNumberpartTitleDOI10.7717peerj.5943citationKeyurlPMIDPMCIDISSN2167-8359languagecollectionsSIJXXBISdateModified2025-03-19T115833ZkeyC795QNTElibraryid5891878metacreatorSummaryHernychovaetal.parsedDate2018numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHernychovaL.RosulekM.KadekA.MareskaV.ChmelikJ.AdamkovaL.GrobarovaV.SebestaO.KukackaZ.SkalaK.SpiwokV.CernyJ.NovakP.ltbgtTheC-TypeLectin-likeReceptorNkrp1bStructuralFeaturesAffectingProteinConformationandInteractionsltbgt.ltigtFEBSOPENBIOltigtltbgt2018ltbgtltigt8ltigt1418.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTheC-typelectin-likereceptorNkrp1bstructuralfeaturesaffectingproteinconformationandinteractionscreatorscreatorTypeauthorfirstNameL.lastNameHernychovacreatorTypeauthorfirstNameM.lastNameRosulekcreatorTypeauthorfirstNameA.lastNameKadekcreatorTypeauthorfirstNameV.lastNameMareskacreatorTypeauthorfirstNameJ.lastNameChmelikcreatorTypeauthorfirstNameL.lastNameAdamkovacreatorTypeauthorfirstNameV.lastNameGrobarovacreatorTypeauthorfirstNameO.lastNameSebestacreatorTypeauthorfirstNameZ.lastNameKukackacreatorTypeauthorfirstNameK.lastNameSkalacreatorTypeauthorfirstNameV.lastNameSpiwokcreatorTypeauthorfirstNameJ.lastNameCernycreatorTypeauthorfirstNameP.lastNameNovakabstractNotedate2018sectionpartNumberpartTitleDOIcitationKeyurlPMIDPMCIDISSN2211-5463languagecollectionsSIJXXBISdateModified2025-03-19T115833ZkeyJM2ZR8JElibraryid5891878metacreatorSummaryFrolikovaetal.parsedDate2018numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtFrolikovaM.Manaskova-PostlerovaP.CernyJ.JankovicovaJ.SimonikO.PohlovaA.SecovaP.AntalikovaJ.Dvorakova-HortovaK.ltbgtCD9andCD81InteractionsandTheirStructuralModellinginSpermPriortoFertilizationltbgt.ltigtINTERNATIONALJOURNALOFMOLECULARSCIENCESltigtltbgt2018ltbgtltigt19ltigt4.ltaclass039zp-DOIURL039href039httpsdoi.org10.3390ijms19041236039gthttpsdoi.org10.3390ijms19041236ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleCD9andCD81InteractionsandTheirStructuralModellinginSpermPriortoFertilizationcreatorscreatorTypeauthorfirstNameMichaelalastNameFrolikovacreatorTypeauthorfirstNamePavlalastNameManaskova-PostlerovacreatorTypeauthorfirstNameJirilastNameCernycreatorTypeauthorfirstNameJanalastNameJankovicovacreatorTypeauthorfirstNameOndrejlastNameSimonikcreatorTypeauthorfirstNameAlzbetalastNamePohlovacreatorTypeauthorfirstNamePetralastNameSecovacreatorTypeauthorfirstNameJanalastNameAntalikovacreatorTypeauthorfirstNameKaterinalastNameDvorakova-HortovaabstractNoteProteinsCD9andCD81aremembersofthetetraspaninsuperfamilyandweredetectedinmammalianspermwheretheyaresuspectedtoformanactivetetraspaninwebandtoparticipateinsperm-eggmembranefusion.TheimportanceofthesetwoproteinsduringtheearlystagesoffertilizationissupportedbythecompletesterilityofCD9CD81doublenullfemalemice.InthisstudytheputativemechanismofCD9CD81involvementintetraspaninwebformationinspermanditsactivitypriortofertilizationwasaddressed.ConfocalmicroscopyandcolocalizationassaywasusedtodetermineamutualCD9CD81localizationvisualisedindetailbysuper-resolutionmicroscopyandtheirinteractionwasaddressbyco-immunoprecipitation.Thespecies-specifictraitsinCD9andCD81distributionduringspermmaturationwerecomparedbetweenmiceandhumans.AmutualpositionofCD9CD81isshowninhumanspermatozoaintheacrosomalcaphoweverinmiceCD9andCD81occupyadistinctarea.DuringtheacrosomereactioninhumanspermonlyCD9isrelocatedcomparedtotherelocationofbothproteinsinmice.ThestructuralmodellingofCD9andCD81homologousandpossiblyheterologousnetworkformationwasusedtoproposetheirlateralCisaswellasTransinteractionswithinthespermmembraneandduringsperm-eggmembranefusion.date2018sectionpartNumberpartTitleDOI10.3390ijms19041236citationKeyurlPMIDPMCIDISSN1422-0067languagecollectionsSIJXXBISdateModified2025-03-19T115833ZkeyJBXDBEA4libraryid5891878metacreatorSummaryStafkovaetal.parsedDate2018numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtStafkovaJ.RadaP.MeloniD.ZarskyV.SmutnaT.ZimmannN.HarantK.PompachP.HrdyI.TachezyJ.ltbgtDynamicSecretomeofTrichomonasVaginalisCaseStudyofBeta-Amylasesltbgt.ltigtMOLECULARampCELLULARPROTEOMICSltigtltbgt2018ltbgtltigt17ltigt2304x2013320.ltaclass039zp-DOIURL039href039httpsdoi.org10.1074mcp.RA117.000434039gthttpsdoi.org10.1074mcp.RA117.000434ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDynamicsecretomeofTrichomonasvaginalisCasestudyofbeta-amylasescreatorscreatorTypeauthorfirstNameJitkalastNameStafkovacreatorTypeauthorfirstNamePetrlastNameRadacreatorTypeauthorfirstNameDionigialastNameMelonicreatorTypeauthorfirstNameVojtechlastNameZarskycreatorTypeauthorfirstNameTamaralastNameSmutnacreatorTypeauthorfirstNameNadinelastNameZimmanncreatorTypeauthorfirstNameKarellastNameHarantcreatorTypeauthorfirstNamePetrlastNamePompachcreatorTypeauthorfirstNameIvanlastNameHrdycreatorTypeauthorfirstNameJanlastNameTachezyabstractNoteThesecretionofvirulencefactorsbyparasiticprotistsintothehostenvironmentplaysafundamentalroleinmultifactorialhost-parasiteinteractions.SeveraleffectorproteinsareknowntobesecretedbyTrichomonasvaginalisahumanparasiteoftheurogenitaltract.HoweveracomprehensiveprofilingoftheT.vaginalissecretomeremainselusiveasdothemechanismsofproteinsecretion.Inthisstudyweusedhigh-resolutionlabel-freequantitativeMStoanalyzetheT.vaginalissecretomeconsideringthatsecretionisatime-andtemperature-dependentprocesstodefinethecutoffforsecretedproteins.Intotalweidentified2072extracellularproteins89ofwhichdisplayedsignificantquantitativeincreasesovertimeat37degreesC.These89bonafidesecretedproteinsweresortedinto13functionalcategories.Approximatelyhalfofthesecretedproteinswerepredictedtopossesstransmembranehelixes.Theseproteinsmainlyincludeputativeadhesinsandleishmaniolysin-likemetallopeptidases.TheotherhalfofthesolubleproteinsincludeseveralnovelpotentialvirulencefactorssuchasDNaseIIpore-formingproteinsandbeta-amylases.Interestinglycurrentbioinformatictoolspredictedthesecretorysignalinonly18oftheidentifiedT.vaginalis-secretedproteins.Thereforeweusedbeta-amylasesasamodeltoinvestigatetheT.vaginalissecretorypathway.Wedemonstratedthattwobeta-amylasesBA1andBA2aretransportedviatheclassicalendoplasmicreticulum-to-GolgipathwaysandinthecaseofBA1weshowedthattheproteinisglycosylatedwithmultipleN-linkedglycansofHex5HexNAc2structure.ThesecretionwasinhibitedbybrefeldinAbutnotbyFLI-06.Anothertwobeta-amylasesBA3andBA4whichareencodedintheT.vaginalisgenomebutabsentfromthesecretomeweretargetedtothelysosomalcompartment.Collectivelyunderdefinedinvitroconditionsouranalysisprovidesacomprehensivesetofconstitutivelysecretedproteinsthatcanserveasareferenceforfuturecomparativestudiesanditprovidesthefirstinformationabouttheclassicalsecretorypathwayinthisparasite.date2018sectionpartNumberpartTitleDOI10.1074mcp.RA117.000434citationKeyurlPMIDPMCIDISSN1535-9476languagecollectionsSIJXXBISdateModified2025-03-19T115833ZkeyJ42SDBGXlibraryid5891878metacreatorSummaryMoravcovaetal.parsedDate2018numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMoravcovaR.MelkesB.NovotnyJ.ltbgtTRHReceptorMobilityinthePlasmaMembraneIsStronglyAffectedbyAgonistBindingandbyInteractionwithSomeCognateSignalingProteinsltbgt.ltigtJOURNALOFRECEPTORSANDSIGNALTRANSDUCTIONltigtltbgt2018ltbgtltigt38ltigt120x201326.ltaclass039zp-DOIURL039href039httpsdoi.org10.108010799893.2017.1398756039gthttpsdoi.org10.108010799893.2017.1398756ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTRHreceptormobilityintheplasmamembraneisstronglyaffectedbyagonistbindingandbyinteractionwithsomecognatesignalingproteinscreatorscreatorTypeauthorfirstNameRadkalastNameMoravcovacreatorTypeauthorfirstNameBarboralastNameMelkescreatorTypeauthorfirstNameJirilastNameNovotnyabstractNoteObjectivesExtensiveresearchhasbeendedicatedtoelucidatingthemechanismsofsignaltransductionthroughdifferentGprotein-coupledreceptorsGPCRs.Howeverrelativelylittleisknownabouttheregulationofreceptormovementwithinthecellmembraneuponligandbinding.Inthisstudywefocusedourattentiononthethyrotropin-releasinghormoneTRHreceptorthattypicallycouplestoGq11proteins.MethodsWemonitoredreceptordiffusionintheplasmamembraneofHEK293cellsstablyexpressingyellowfluorescentproteinYFP-taggedTRHreceptorTRHR-YFPbyfluorescencerecoveryafterphotobleachingFRAP.ResultsFRAPanalysisindicatedthatthelateralmovementoftheTRHreceptorwasmarkedlyreduceduponTRHbindingasthevalueofitsdiffusioncoefficientfelldownby55.ThiseffectwaspreventedbytheadditionoftheTRHreceptorantagonistmidazolam.WealsofoundthatsiRNA-mediatedknock-downofGq11alphaGbetabeta-arrestin2andphospholipaseCbeta1butnotofGialpha1beta-arrestin1orGprotein-coupledreceptorkinase2resultedinasignificantdecreaseintherateofTRHR-YFPdiffusionindicatingtheinvolvementoftheformerproteinsintheregulationofTRHreceptorbehavior.TheobservedpartialreductionoftheTRHR-YFPmobilefractioncausedbydown-regulationofGialpha1andbeta-arrestin1suggeststhattheseproteinsmayalsoplaydistinctrolesinTHRreceptor-mediatedsignaling.ConclusionTheseresultsdemonstrateforthefirsttimethatnotonlyagonistbindingbutalsoabundanceofsomesignalingproteinsmaystronglyaffectTRHreceptordynamicsintheplasmamembrane.date2018sectionpartNumberpartTitleDOI10.108010799893.2017.1398756citationKeyurlPMIDPMCIDISSN1079-9893languagecollectionsSIJXXBISdateModified2025-03-19T115833Z
1.
Hubka, V.; Barrs, V.; Dudova, Z.; Sklenar, F.; Kubatova, A.; Matsuzawa, T.; Yaguchi, T.; Horie, Y.; Novakova, A.; Frisvad, J. C.; Talbot, J. J.; Kolarik, M. Unravelling Species Boundaries in the Aspergillus Viridinutans Complex (Section Fumigati): Opportunistic Human and Animal Pathogens Capable of Interspecific Hybridization. Persoonia 2018, 41, 142–174. https://doi.org/10.3767/persoonia.2018.41.08.
1.
Vavra, J.; Fiala, I.; Krylova, P.; Petrusek, A.; Hylis, M. Molecular and Structural Assessment of Microsporidia Infecting Daphnids: The “Obtusa-like” Microsporidia, a Branch of the Monophyletic Agglomeratidae Glade, with the Establishment of a New Genus Conglomerata. J. Invertebr. Pathol. 2018, 159, 95–104. https://doi.org/10.1016/j.jip.2018.10.003.
1.
Sahi, V. P.; Cifrova, P.; Garcia-Gonzalez, J.; Baby, I. K.; Mouille, G.; Gineau, E.; Mueller, K.; Baluska, F.; Soukup, A.; Petrasek, J.; Schwarzerova, K. Arabidopsis Thaliana Plants Lacking the ARP2/3 Complex Show Defects in Cell Wall Assembly and Auxin Distribution. Ann. Bot. 2018, 122 (5), 777–789. https://doi.org/10.1093/aob/mcx178.
1.
Rotterova, J.; Bourland, W.; Cepicka, I. Tropidoatractidae Fam. Nov., a Deep Branching Lineage of Metopida (Armophorea, Ciliophora) Found in Diverse Habitats and Possessing Prokaryotic Symbionts. Protist 2018, 169 (3), 362–405. https://doi.org/10.1016/j.protis.2018.04.003.
1.
Vilimova, J.; Krizkova, P.; Jansta, P.; McPherson, J. E. Sexual Dimorphism in External Scent Efferent System of Metathoracic Scent Glands in Aradidae: First Evidence within Heteroptera. Zool. Anz. 2018, 274, 115–122. https://doi.org/10.1016/j.jcz.2018.02.001.
1.
Agarwal, K.; Macháň, R.; Prasad, D. K. Non-Heuristic Automatic Techniques for Overcoming Low Signal-to-Noise-Ratio Bias of Localization Microscopy and Multiple Signal Classification Algorithm. Sci Rep 2018, 8 (1), 4988. https://doi.org/10.1038/s41598-018-23374-7.
1.
Klima, P.; Lankova, M.; Vandenbussche, F.; Straeten, D. V. D.; Petrasek, J. Silver Ions Increase Plasma Membrane Permeability through Modulation of Intracellular Calcium Levels in Tobacco BY-2 Cells. PLANT CELL REPORTS 2018, 37 (5), 809–818. https://doi.org/10.1007/s00299-018-2269-6.
1.
Angelini, J.; Vosolsobe, S.; Skupa, P.; Ho, A. Y. Y.; Bellinvia, E.; Valentova, O.; Marc, J. Phospholipase D Delta Assists to Cortical Microtubule Recovery after Salt Stress. PROTOPLASMA 2018, 255 (4), 1195–1204. https://doi.org/10.1007/s00709-018-1204-6.
1.
Svobodova, I.; Bhattaracharya, A.; Ivetic, M.; Bendova, Z.; Zemkova, H. Circadian ATP Release in Organotypic Cultures of the Rat Suprachiasmatic Nucleus Is Dependent on P2X7 and P2Y Receptors. FRONTIERS IN PHARMACOLOGY 2018, 9. https://doi.org/10.3389/fphar.2018.00192.
1.
Merwe, I. van der; Lukáts, A.; Bláhová, V.; Oosthuizen, M. K.; Bennett, N. C.; Nemec, P. The Topography of Rods, Cones and Intrinsically Photosensitive Retinal Ganglion Cells in the Retinas of a Nocturnal (Micaelamys Namaquensis) and a Diurnal (Rhabdomys Pumilio) Rodent. PLOS ONE 2018, 13 (8). https://doi.org/10.1371/journal.pone.0202106.
1.
Kulich, I.; Vojtíková, Z.; Sabol, P.; Ortmannová, J.; Nedela, V.; Tihlariková, E.; Zársky, V. Exocyst Subunit EXO70H4 Has a Specific Role in Callose Synthase Secretion and Silica Accumulation. PLANT PHYSIOLOGY 2018, 176 (3), 2040–2051. https://doi.org/10.1104/pp.17.01693.
1.
Pichrtová, M.; Holzinger, A.; Kulichová, J.; Rysánek, D.; Soljaková, T.; Trumhová, K.; Nemcova, Y. Molecular and Morphological Diversity of Zygnema a Zygnemopsis (Zygnematophyceae, Streptophyta) from Svalbard (High Arctic). EUROPEAN JOURNAL OF PHYCOLOGY 2018, 53 (4), 492–508. https://doi.org/10.1080/09670262.2018.1476920.
1.
Stáhlavsky, F.; Opatova, V.; Just, P.; Lotz, L. N.; Haddad, C. R. Molecular Technique Reveals High Variability of 18S rDNA Distribution in Harvestmen (Opiliones, Phalangiidae) from South Africa. COMPARATIVE CYTOGENETICS 2018, 12 (1), 41–59. https://doi.org/10.3897/CompCytogen.v12i1.21744.
1.
Stáhlavsky, F.; Stundlová, J.; Lowe, G.; Stockmann, M.; Kovarík, F. Application of Cytogenetic Markers in the Taxonomy of Flat Rock Scorpions (Scorpiones: Hormuridae), with the Description of Hadogenes Weygoldti Sp n. ZOOLOGISCHER ANZEIGER 2018, 273, 173–182. https://doi.org/10.1016/j.jcz.2018.01.007.
1.
Hirman, M.; Kotyk, M.; Varadinová, Z. K.; Stáhlavsky, F. FIRST CYTOGENETIC STUDY OF A MEMBER OF THE HARVESTMAN FAMILY NEOGOVEIDAE (OPILIONES: CYPHOPHTHALMI: STERNOPHTHALMI) FROM CAMEROON WITH DESCRIPTION OF A NEW SPECIES PAROGOVIA PARASIRONOIDES SP. NOV. ANNALES ZOOLOGICI 2018, 68 (4), 867–877. https://doi.org/10.3161/00034541ANZ2018.68.4.010.
1.
Nedvedova, I.; Kolar, D.; Elsnicova, B.; Hornikova, D.; Novotny, J.; Kalous, M.; Pravenec, M.; Neckar, J.; Kolar, F.; Zurmanova, J. M. Mitochondrial Genome Modulates Myocardial Akt/Glut/HK Salvage Pathway in Spontaneously Hypertensive Rats Adapted to Chronic Hypoxia. PHYSIOLOGICAL GENOMICS 2018, 50 (7), 532–541. https://doi.org/10.1152/physiolgenomics.00040.2017.
1.
Sladek, V.; Sabolova, V.; Sebesta, O.; Zikmund, T.; Kaiser, J.; Cerevkova, S. Effect of Deriving Periosteal and Endosteal Contours from microCT Scans on Computation of Cross-Sectional Properties in Non-Adults: The Femur. JOURNAL OF ANATOMY 2018, 233 (3), 381–393. https://doi.org/10.1111/joa.12835.
1.
Pohl, H.; Batelka, J.; Prokop, J.; Mueller, P.; Yavorskaya, M. I.; Beutel, R. G. A Needle in a Haystack: Mesozoic Origin of Parasitism in Strepsiptera Revealed by First Definite Cretaceous Primary Larva (Insecta). PEERJ 2018, 6. https://doi.org/10.7717/peerj.5943.
1.
Hernychova, L.; Rosulek, M.; Kadek, A.; Mareska, V.; Chmelik, J.; Adamkova, L.; Grobarova, V.; Sebesta, O.; Kukacka, Z.; Skala, K.; Spiwok, V.; Cerny, J.; Novak, P. The C-Type Lectin-like Receptor Nkrp1b: Structural Features Affecting Protein Conformation and Interactions. FEBS OPEN BIO 2018, 8 (1), 418.
1.
Frolikova, M.; Manaskova-Postlerova, P.; Cerny, J.; Jankovicova, J.; Simonik, O.; Pohlova, A.; Secova, P.; Antalikova, J.; Dvorakova-Hortova, K. CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2018, 19 (4). https://doi.org/10.3390/ijms19041236.
1.
Stafkova, J.; Rada, P.; Meloni, D.; Zarsky, V.; Smutna, T.; Zimmann, N.; Harant, K.; Pompach, P.; Hrdy, I.; Tachezy, J. Dynamic Secretome of Trichomonas Vaginalis: Case Study of Beta-Amylases. MOLECULAR & CELLULAR PROTEOMICS 2018, 17 (2), 304–320. https://doi.org/10.1074/mcp.RA117.000434.
1.
Moravcova, R.; Melkes, B.; Novotny, J. TRH Receptor Mobility in the Plasma Membrane Is Strongly Affected by Agonist Binding and by Interaction with Some Cognate Signaling Proteins. JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION 2018, 38 (1), 20–26. https://doi.org/10.1080/10799893.2017.1398756.
2017
5891878
V9I2F6IG
1
https://raw.githubusercontent.com/Schebique/vmcf-konfmi/refs/heads/main/vmcf-web-style.csl
50
date
desc
4983
https://web.natur.cuni.cz/sekce-bi/VMCF/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%227KW3E7AP%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Bourland%20et%20al.%22%2C%22parsedDate%22%3A%222017-10%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BBourland%2C%20W.%3B%20Rotterova%2C%20J.%3B%20Cepicka%2C%20I.%20%26lt%3Bb%26gt%3BMorphologic%20and%20Molecular%20Characterization%20of%20Seven%20Species%20of%20the%20Remarkably%20Diverse%20and%20Widely%20Distributed%20Metopid%20Genus%20Urostomides%20Jankowski%2C%201964%20%28Armophorea%2C%20Ciliophora%29%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BEur.%20J.%20Protistol.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2017%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B61%26lt%3B%5C%2Fi%26gt%3B%2C%20194%26%23x2013%3B232.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.ejop.2017.07.003%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.ejop.2017.07.003%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Morphologic%20and%20molecular%20characterization%20of%20seven%20species%20of%20the%20remarkably%20diverse%20and%20widely%20distributed%20metopid%20genus%20Urostomides%20Jankowski%2C%201964%20%28Armophorea%2C%20Ciliophora%29%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22William%22%2C%22lastName%22%3A%22Bourland%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Johana%22%2C%22lastName%22%3A%22Rotterova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ivan%22%2C%22lastName%22%3A%22Cepicka%22%7D%5D%2C%22abstractNote%22%3A%22The%20free-living%20ciliates%20of%20the%20order%20Metopida%20Jankowski%2C%201980%20are%20pivotal%20players%20in%20the%20microbial%20food%20web%20of%20the%20sulfuretum%2C%20acting%20as%20hosts%20to%20prokaryotic%20endo-%20and%20ectosymbionts.%20They%20are%20also%20of%20interest%20in%20the%20study%20of%20the%20function%20and%20evolution%20of%20their%20mitochondrion-related%20organelle%2C%20the%20hydrogenosome.%20The%20taxonomy%20and%20phylogeny%20of%20this%20group%20remains%20confused%2C%20due%2C%20in%20large%20part%2C%20to%20the%20fact%20that%20most%20of%20its%20taxa%20have%20not%20been%20characterized%20by%20modern%20methods%20including%20molecular%20sequencing.%20In%20this%20report%20we%20provide%20morphologic%20and%20molecular%20characterization%20of%20seven%20taxa%20from%20the%20poorly-known%20resurrected%20genus%20Urostomides%20obtained%20in%20the%20course%20of%20broad%20geographic%20sampling.%20Foissner%20%282016%29%20established%20the%20family%20Apometopidae%20to%20include%20Apometopus%20%28a%20junior%20synonym%20of%20Urostomides%29%20and%20Cirranter%20Jankowski%2C%201964.%20These%20two%20genera%20differ%20from%20all%20other%20metopid%20genera%20in%20having%20a%20four-rowed%20perizonal%20ciliary%20stripe%2C%20the%20only%20currently%20recognizable%20morphologic%20synapomorphy%20for%20the%20family.%20The%20members%20of%20Urostomides%20show%20remarkable%20morphologic%20diversity.%20The%20genus%20has%20a%20broad%20geographic%20distribution%2C%20occurring%20on%20six%20continents.%20Urostomides%20species%20form%20a%20strongly%20supported%20clade%20in%20phylogenetic%20analyses.%20Relationships%20within%20the%20genus%20itself%20are%20less%20clearly%20resolved.%20The%20diagnoses%20of%20Apometopidae%20and%20Urostomides%20are%20emended.%20%28C%29%202017%20Elsevier%20GmbH.%20All%20rights%20reserved.%22%2C%22date%22%3A%22OCT%202017%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.ejop.2017.07.003%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220932-4739%2C%201618-0429%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%22V9I2F6IG%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A32%3A18Z%22%7D%7D%2C%7B%22key%22%3A%22VMYQ49YH%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Vavra%20et%20al.%22%2C%22parsedDate%22%3A%222017-10%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BVavra%2C%20J.%3B%20Hylis%2C%20M.%3B%20Fiala%2C%20I.%3B%20Sacherova%2C%20V.%3B%20Vossbrinck%2C%20C.%20R.%20%26lt%3Bb%26gt%3BMicrosporidian%20Genus%20Berwaldia%20%28Opisthosporidia%2C%20Microsporidia%29%2C%20Infecting%20Daphnids%20%28Crustacea%2C%20Branchiopoda%29%3A%20Biology%2C%20Structure%2C%20Molecular%20Phylogeny%20and%20Description%20of%20Two%20New%20Species%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BEur.%20J.%20Protistol.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2017%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B61%26lt%3B%5C%2Fi%26gt%3B%2C%201%26%23x2013%3B12.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.ejop.2017.07.005%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.ejop.2017.07.005%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Microsporidian%20genus%20Berwaldia%20%28Opisthosporidia%2C%20Microsporidia%29%2C%20infecting%20daphnids%20%28Crustacea%2C%20Branchiopoda%29%3A%20Biology%2C%20structure%2C%20molecular%20phylogeny%20and%20description%20of%20two%20new%20species%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jiri%22%2C%22lastName%22%3A%22Vavra%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Miroslav%22%2C%22lastName%22%3A%22Hylis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ivan%22%2C%22lastName%22%3A%22Fiala%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Veronika%22%2C%22lastName%22%3A%22Sacherova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charles%20R.%22%2C%22lastName%22%3A%22Vossbrinck%22%7D%5D%2C%22abstractNote%22%3A%22Structural%2C%20molecular%20and%20life%20cycle%20data%20are%20presented%20for%20two%20microsporidian%20species%20of%20the%20genus%20Berwaldia%20B.%20singtllaris%20Larsson%2C%201981%20%28type%20species%20of%20the%20genus%29%20and%20B.%20schaefernai%20Vavra%20and%20Larsson%2C%201994%2C%20parasites%20of%20Daphnia%20pulex%20Leydig%2C%201860%20and%20Daphnia%20galeata%20Sars%2C%201863%2C%20respectively%2C%20Analysis%20of%20the%20SSU%20rDNA%20gene%20confirmed%20the%20species%20status%20of%20both%20species%20and%20showed%20that%20the%20GenBank%20sequence%20data%20submitted%20previously%20in%20GenBank%20for%20the%20genus%20Berwaldia%2C%20are%20from%20microsporidia%20that%20are%20not%20Berwaldia.%20Correct%20SSU%20rDNA%20gene%20sequences%20for%20B.%20schaefernai%20and%20B.%20singularis%20are%20now%20deposited%20in%20GenBank%2C%20The%20life%20cycle%20of%20these%20two%20species%20appears%20incomplete%20as%20the%20spores%20collected%20from%20their%20respective%20infected%20hosts%20will%20not%20infect%20the%20same%20host%20when%20fed%20per%20os%2C%20B.%20schaefernai%20appears%20as%20a%20frequent%20parasite%20of%20Daphnia%20longispina%5C%2Fgaleata%20complex%20daphnids%2C%20influencing%20the%20behaviour%20of%20the%20infected%20host.%20In%20addition%2C%20two%20new%20species%2C%20of%20Berwaldia%2C%20one%20infecting%20fat%20body%20tissues%20of%20Daphnia%20longispinalgaleata%20complex%2C%20and%20the%20other%2C%20infecting%20hypodermis%20and%20fat%20cells%20of%20Simocephalus%20vetulus%20%28O.%20F.%20Muller%2C%201776%29%20are%20described.%20%28C%29%202017%20Elsevier%20GmbH.%20All%20rights%20reserved.%22%2C%22date%22%3A%22OCT%202017%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.ejop.2017.07.005%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.webofscience.com%5C%2Fapi%5C%2Fgateway%3FGWVersion%3D2%26SrcAuth%3DGetFTR%26SrcApp%3DWOS%26DestURL%3Dhttps%253A%252F%252Fct.prod.getft.io%252FY2xhcml2YXRlLGVsc2V2aWVyLGh0dHBzOi8vd3d3LnNjaWVuY2VkaXJlY3QuY29tL3NjaWVuY2UvYXJ0aWNsZS9waWkvUzA5MzI0NzM5MTczMDEwNTA_cGVzPXZvcg._jpddeO1siQImthuG6MCL01crwHAyIFke5r2e4otv0g%26DestApp%3DGetFTR%26SrcItemId%3DWOS%3A000416534000001%26SrcAppSID%3DEUW1ED0AD5l35ynCpYiKbmzlvxhzS%26HMAC%3DqP9KQx7OoW23yI7GeUFQL%252FtfPxBdpEgapltio4jvHIc%253D%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220932-4739%2C%201618-0429%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%22V9I2F6IG%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A31%3A36Z%22%7D%7D%2C%7B%22key%22%3A%22WIGDRRJS%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Sklenar%20et%20al.%22%2C%22parsedDate%22%3A%222017-09%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BSklenar%2C%20F.%3B%20Jurjevic%2C%20Z.%3B%20Zalar%2C%20P.%3B%20Frisvad%2C%20J.%20C.%3B%20Visagie%2C%20C.%20M.%3B%20Kolarik%2C%20M.%3B%20Houbraken%2C%20J.%3B%20Chen%2C%20A.%20J.%3B%20Yilmaz%2C%20N.%3B%20Seifert%2C%20K.%20A.%3B%20Coton%2C%20M.%3B%20Deniel%2C%20F.%3B%20Gunde-Cimerman%2C%20N.%3B%20Samson%2C%20R.%20A.%3B%20Peterson%2C%20S.%20W.%3B%20Hubka%2C%20V.%20%26lt%3Bb%26gt%3BPhylogeny%20of%20Xerophilic%20Aspergilli%20%28Subgenus%20Aspergillus%29%20and%20Taxonomic%20Revision%20of%20Section%20Restricti%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BStud.%20Mycol.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2017%26lt%3B%5C%2Fb%26gt%3B%2C%20No.%2088%2C%20161%26%23x2013%3B236.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.simyco.2017.09.002%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.simyco.2017.09.002%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Phylogeny%20of%20xerophilic%20aspergilli%20%28subgenus%20Aspergillus%29%20and%20taxonomic%20revision%20of%20section%20Restricti%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22Sklenar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Z.%22%2C%22lastName%22%3A%22Jurjevic%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Zalar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20C.%22%2C%22lastName%22%3A%22Frisvad%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20M.%22%2C%22lastName%22%3A%22Visagie%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Kolarik%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Houbraken%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20J.%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22N.%22%2C%22lastName%22%3A%22Yilmaz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Seifert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Coton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22Deniel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22N.%22%2C%22lastName%22%3A%22Gunde-Cimerman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20A.%22%2C%22lastName%22%3A%22Samson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%20W.%22%2C%22lastName%22%3A%22Peterson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Hubka%22%7D%5D%2C%22abstractNote%22%3A%22Aspergillus%20section%20Restricti%20together%20with%20sister%20section%20Aspergillus%20%28formerly%20Eurotium%29%20comprises%20xerophilic%20species%2C%20that%20are%20able%20to%20grow%20on%20substrates%20with%20low%20water%20activity%20and%20in%20extreme%20environments.%20We%20adressed%20the%20monophyly%20of%20both%20sections%20within%20subgenus%20Aspergillus%20and%20applied%20a%20multidisciplinary%20approach%20for%20definition%20of%20species%20boundaries%20in%20sect.%20Restricti.%20The%20monophyly%20of%20sections%20Aspergillus%20and%20Restricti%20was%20tested%20on%20a%20set%20of%20102%20isolates%20comprising%20all%20currently%20accepted%20species%20and%20was%20strongly%20supported%20by%20Maximum%20likelihood%20%28ML%29%20and%20Bayesian%20inferrence%20%28BI%29%20analysis%20based%20on%20beta-tubulin%20%28benA%29%2C%20calmodulin%20%28CaM%29%20and%20RNA%20polymerase%20II%20second%20largest%20subunit%20%28RPB2%29%20loci.%20More%20than%20300%20strains%20belonging%20to%20sect.%20Restricti%20from%20various%20isolation%20sources%20and%20four%20continents%20were%20characterized%20by%20DNA%20sequencing%2C%20and%20193%20isolates%20were%20selected%20for%20phylogenetic%20analyses%20and%20phenotypic%20studies.%20Species%20delimitation%20methods%20based%20on%20multispecies%20coalescent%20model%20were%20employed%20on%20DNA%20sequences%20from%20four%20loci%2C%20i.e.%2C%20ID%20region%20of%20rDNA%20%28ITS%20%2B%2028S%29%2C%20CaM%2C%20benA%20and%20RPB2%2C%20and%20supported%20recognition%20of%2021%20species%2C%20including%2014%20new.%20All%20these%20species%20were%20also%20strongly%20supported%20in%20ML%20and%20BI%20analyses.%20All%20recognised%20species%20can%20be%20reliably%20identified%20by%20all%20four%20examined%20genetic%20loci.%20Phenotype%20analysis%20was%20performed%20to%20support%20the%20delimitation%20of%20new%20species%20and%20includes%20colony%20characteristics%20on%20seven%20cultivation%20media%20incubated%20at%20several%20temperatures%2C%20growth%20on%20an%20osmotic%20gradient%20%28six%20media%20with%20NaCl%20concentration%20from%200%20to%2025%20%25%29%20and%20analysis%20of%20morphology%20including%20scanning%20electron%20microscopy.%20The%20micromorphology%20of%20conidial%20heads%2C%20vesicle%20dimensions%2C%20temperature%20profiles%20and%20growth%20parameters%20in%20osmotic%20gradient%20were%20useful%20criteria%20for%20species%20identification.%20The%20vast%20majority%20of%20species%20in%20sect.%20Restricti%20produce%20asperglaucide%2C%20asperphenamate%20or%20both%20in%20contrast%20to%20species%20in%20sect.%20Aspergillus.%20Mycophenolic%20acid%20was%20detected%20for%20the%20first%20time%20in%20at%20least%20six%20members%20of%20the%20section.%20The%20ascomata%20of%20A.%20halophilicus%20do%20not%20contain%20auroglaucin%2C%20epiheveadride%20or%20flavoglaucin%20which%20are%20common%20in%20sect.%20Aspergillus%2C%20but%20shares%20the%20echinulins%20with%20sect.%20Aspergillus.%22%2C%22date%22%3A%22SEP%202017%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.simyco.2017.09.002%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220166-0616%2C%201872-9797%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%22V9I2F6IG%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A31%3A53Z%22%7D%7D%2C%7B%22key%22%3A%2283C3UQ6E%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Chen%20et%20al.%22%2C%22parsedDate%22%3A%222017-09%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BChen%2C%20A.%20J.%3B%20Hubka%2C%20V.%3B%20Frisvad%2C%20J.%20C.%3B%20Visagie%2C%20C.%20M.%3B%20Houbraken%2C%20J.%3B%20Meijer%2C%20M.%3B%20Varga%2C%20J.%3B%20Demirel%2C%20R.%3B%20Jurjevic%2C%20Z.%3B%20Kubatova%2C%20A.%3B%20Sklenar%2C%20F.%3B%20Zhou%2C%20Y.%20G.%3B%20Samson%2C%20R.%20A.%20%26lt%3Bb%26gt%3BPolyphasic%20Taxonomy%20of%20Aspergillus%20Section%20Aspergillus%20%28Formerly%20Eurotium%29%2C%20and%20Its%20Occurrence%20in%20Indoor%20Environments%20and%20Food%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BStud.%20Mycol.%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2017%26lt%3B%5C%2Fb%26gt%3B%2C%20No.%2088%2C%2037%26%23x2013%3B135.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.simyco.2017.07.001%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.simyco.2017.07.001%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Polyphasic%20taxonomy%20of%20Aspergillus%20section%20Aspergillus%20%28formerly%20Eurotium%29%2C%20and%20its%20occurrence%20in%20indoor%20environments%20and%20food%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20J.%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%22%2C%22lastName%22%3A%22Hubka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20C.%22%2C%22lastName%22%3A%22Frisvad%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20M.%22%2C%22lastName%22%3A%22Visagie%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Houbraken%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Meijer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Varga%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Demirel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Z.%22%2C%22lastName%22%3A%22Jurjevic%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Kubatova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22Sklenar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Y.%20G.%22%2C%22lastName%22%3A%22Zhou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20A.%22%2C%22lastName%22%3A%22Samson%22%7D%5D%2C%22abstractNote%22%3A%22Aspergillus%20section%20Aspergillus%20%28formerly%20the%20genus%20Eurotium%29%20includes%20xerophilic%20species%20with%20uniseriate%20conidiophores%2C%20globose%20to%20subglobose%20vesicles%2C%20green%20conidia%20and%20yellow%2C%20thin%20walled%20eurotium-like%20ascomata%20with%20hyaline%2C%20lenticular%20ascospores.%20In%20the%20present%20study%2C%20a%20polyphasic%20approach%20using%20morphological%20characters%2C%20extrolites%2C%20physiological%20characters%20and%20phylogeny%20was%20applied%20to%20investigate%20the%20taxonomy%20of%20this%20section.%20Over%20500%20strains%20from%20various%20culture%20collections%20and%20new%20isolates%20obtained%20from%20indoor%20environments%20and%20a%20wide%20range%20of%20substrates%20all%20over%20the%20world%20were%20identified%20using%20calmodulin%20gene%20sequencing.%20Of%20these%2C%20163%20isolates%20were%20subjected%20to%20molecular%20phylogenetic%20analyses%20using%20sequences%20of%20ITS%20rDNA%2C%20partial%20beta-tubulin%20%28BenA%29%2C%20calmodulin%20%28CaM%29%20and%20RNA%20polymerase%20II%20second%20largest%20subunit%20%28RPB2%29%20genes.%20Colony%20characteristics%20were%20documented%20on%20eight%20cultivation%20media%2C%20growth%20parameters%20at%20three%20incubation%20temperatures%20were%20recorded%20and%20micromorphology%20was%20examined%20using%20light%20microscopy%20as%20well%20as%20scanning%20electron%20microscopy%20to%20illustrate%20and%20characterize%20each%20species.%20Many%20specific%20extrolites%20were%20extracted%20and%20identified%20from%20cultures%2C%20including%20echinulins%2C%20epiheveadrides%2C%20auroglaucins%20and%20anthraquinone%20bisanthrons%2C%20and%20to%20be%20consistent%20in%20strains%20of%20nearly%20all%20species.%20Other%20extrolites%20are%20species-specific%2C%20and%20thus%20valuable%20for%20identification.%20Several%20extrolites%20show%20antioxidant%20effects%2C%20which%20may%20be%20nutritionally%20beneficial%20in%20food%20and%20beverages.%20Important%20mycotoxins%20in%20the%20strict%20sense%2C%20such%20as%20sterigmatocystin%2C%20aflatoxins%2C%20ochratoxins%2C%20citrinin%20were%20not%20detected%20despite%20previous%20reports%20on%20their%20production%20in%20this%20section.%20Adopting%20a%20polyphasic%20approach%2C%2031%20species%20are%20recognized%2C%20including%20nine%20new%20species.%20ITS%20is%20highly%20conserved%20in%20this%20section%20and%20does%20not%20distinguish%20species.%20All%20species%20can%20be%20differentiated%20using%20CaM%20or%20RPB2%20sequences.%20For%20BenA%2C%20Aspergillus%20brunneus%20and%20A.%20niveoglaucus%20share%20identical%20sequences.%20Ascospores%20and%20conidia%20morphology%2C%20growth%20rates%20at%20different%20temperatures%20are%20most%20useful%20characters%20for%20phenotypic%20species%20identification.%22%2C%22date%22%3A%22SEP%202017%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.simyco.2017.07.001%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.webofscience.com%5C%2Fapi%5C%2Fgateway%3FGWVersion%3D2%26SrcAuth%3DGetFTR%26SrcApp%3DWOS%26DestURL%3Dhttps%253A%252F%252Fct.prod.getft.io%252FY2xhcml2YXRlLHVuZGVmaW5lZCxodHRwOi8vZHguZG9pLm9yZy8xMC4xMDE2L2ouc2lteWNvLjIwMTcuMDcuMDAx.YvH7N4rCsmffzRv4CKq5nmIzyPFlhUX2MrkJsKr0t6c%26DestApp%3DGetFTR%26SrcItemId%3DWOS%3A000418560600002%26SrcAppSID%3DEUW1ED0AD5l35ynCpYiKbmzlvxhzS%26HMAC%3DwTHlEYRePcH6nhNGiLFATifEolp2MV96wAmJLzCjVhE%253D%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220166-0616%2C%201872-9797%22%2C%22language%22%3A%22English%22%2C%22collections%22%3A%5B%22V9I2F6IG%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A31%3A45Z%22%7D%7D%2C%7B%22key%22%3A%22M4LSYJXP%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Pilarska%20et%20al.%22%2C%22parsedDate%22%3A%222017-01-20%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BPilarska%2C%20D.%3B%20Takov%2C%20D.%3B%20Hyli%26%23x161%3B%2C%20M.%3B%20Radek%2C%20R.%3B%20Fiala%2C%20I.%3B%20Solter%2C%20L.%3B%20Linde%2C%20A.%20%26lt%3Bb%26gt%3BNatural%20Occurrence%20of%20Microsporidia%20Infecting%20Lepidoptera%20in%20Bulgaria%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BActa%20Parasitologica%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2017%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B62%26lt%3B%5C%2Fi%26gt%3B%20%284%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1515%5C%2Fap-2017-0104%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1515%5C%2Fap-2017-0104%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Natural%20occurrence%20of%20microsporidia%20infecting%20Lepidoptera%20in%20Bulgaria%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniela%22%2C%22lastName%22%3A%22Pilarska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Danail%22%2C%22lastName%22%3A%22Takov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Miroslav%22%2C%22lastName%22%3A%22Hyli%5Cu0161%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Renate%22%2C%22lastName%22%3A%22Radek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ivan%22%2C%22lastName%22%3A%22Fiala%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Leellen%22%2C%22lastName%22%3A%22Solter%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andreas%22%2C%22lastName%22%3A%22Linde%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%5Cn%20%20%20%20%20%20%20%20%20%20%20%20We%20examined%2034%20lepidopteran%20species%20belonging%20to%2012%20families%20to%20determine%20presence%20and%20prevalence%20of%20microsporidian%20pathogens.%20The%20insects%20were%20collected%20from%20May%202009%20to%20July%202012%20from%2044%20sites%20in%20Bulgaria.%22%2C%22date%22%3A%222017-01-20%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1515%5C%2Fap-2017-0104%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.degruyter.com%5C%2Fdoi%5C%2F10.1515%5C%2Fap-2017-0104%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221896-1851%2C%201230-2821%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22V9I2F6IG%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A32%3A48Z%22%7D%7D%2C%7B%22key%22%3A%22PSTSSMFW%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Rimnacova%20et%20al.%22%2C%22parsedDate%22%3A%222017%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BRimnacova%2C%20J.%3B%20Mikes%2C%20L.%3B%20Turjanicova%2C%20L.%3B%20Bulantova%2C%20J.%3B%20Horak%2C%20P.%20%26lt%3Bb%26gt%3BChanges%20in%20Surface%20Glycosylation%20and%20Glycocalyx%20Shedding%20in%20Trichobilharzia%20Regenti%20%28Schistosomatidae%29%20during%20the%20Transformation%20of%20Cercaria%20to%20Schistosomulum%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BPLOS%20ONE%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2017%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B12%26lt%3B%5C%2Fi%26gt%3B%20%283%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0173217%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0173217%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Changes%20in%20surface%20glycosylation%20and%20glycocalyx%20shedding%20in%20Trichobilharzia%20regenti%20%28Schistosomatidae%29%20during%20the%20transformation%20of%20cercaria%20to%20schistosomulum%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jana%22%2C%22lastName%22%3A%22Rimnacova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Libor%22%2C%22lastName%22%3A%22Mikes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Libuse%22%2C%22lastName%22%3A%22Turjanicova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jana%22%2C%22lastName%22%3A%22Bulantova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petr%22%2C%22lastName%22%3A%22Horak%22%7D%5D%2C%22abstractNote%22%3A%22The%20invasive%20larvae%20%28cercariae%29%20of%20schistosomes%20penetrate%20the%20skin%20of%20their%20definitive%20hosts.%20During%20the%20invasion%2C%20they%20undergo%20dramatic%20ultrastructural%20and%20physiological%20transitions.%20These%20changes%20result%20in%20the%20development%20of%20the%20subsequent%20stage%2C%20schistosomulum%2C%20which%20migrates%20through%20host%20tissues%20in%20close%20contact%20with%20host%26%23039%3Bs%20immune%20system.%20One%20of%20the%20striking%20changes%20in%20the%20transforming%20cercariae%20is%20the%20shedding%20of%20their%20thick%20tegumental%20glycocalyx%2C%20which%20represents%20an%20immunoattractive%20structure%3B%20therefore%20its%20removal%20helps%20cercariae%20to%20avoid%20immune%20attack.%20A%20set%20of%20commercial%20fluorescently%20labeled%20lectin%20probes%2C%20their%20saccharide%20inhibitors%20and%20monoclonal%20antibodies%20against%20the%20trisaccharide%20Lewis-X%20antigen%20%28Le%28X%29%2C%20CD15%29%20were%20used%20to%20characterize%20changes%20in%20the%20surface%20saccharide%20composition%20of%20the%20neuropathogenic%20avian%20schistosome%20Trichobilharzia%20regenti%20during%20the%20transformation%20of%20cercariae%20to%20schistosomula%2C%20both%20in%20vitro%20and%20in%20vivo.%20The%20effect%20of%20various%20lectins%20on%20glycocalyx%20shedding%20was%20evaluated%20microscopically.%20The%20involvement%20of%20peptidases%20and%20their%20inhibitors%20on%20the%20shedding%20of%20glycocalyx%20was%20investigated%20using%20T.%20regenti%20recombinant%20cathepsin%20B2%20and%20a%20set%20of%20peptidase%20inhibitors.%20The%20surface%20glycocalyx%20of%20T.%20regenti%20cercariae%20was%20rich%20in%20fucose%20and%20mannose%5C%2Fglucose%20residues.%20After%20the%20transformation%20of%20cercariae%20in%20vitro%20or%20in%20vivo%20within%20their%20specific%20duck%20host%2C%20reduction%20and%20vanishing%20of%20these%20epitopes%20was%20observed%2C%20and%20galactose%5C%2FN-acetylgalactosamine%20emerged.%20The%20presence%20of%20Le%28X%29%20was%20not%20observed%20on%20the%20cercariae%2C%20but%20the%20antigen%20was%20gradually%20expressed%20from%20the%20anterior%20part%20of%20the%20body%20in%20the%20developing%20schistosomula.%20Some%20lectins%20which%20bind%20to%20the%20cercarial%20surface%20also%20induced%20secretion%20from%20the%20acetabular%20penetration%20glands.%20Seven%20lectins%20induced%20the%20shedding%20of%20glycocalyx%20by%20cercariae%2C%20among%20which%20five%20bound%20strongly%20to%20cercarial%20surface%3B%20the%20effect%20could%20be%20blocked%20by%20saccharide%20inhibitors.%20Mannose-binding%20protein%2C%20part%20of%20the%20lectin%20pathway%20of%20the%20complement%20system%2C%20also%20bound%20to%20cercariae%20and%20schistosomula%2C%20but%20had%20little%20effect%20on%20glycocalyx%20shedding.%20Our%20study%20did%20not%20confirm%20the%20involvement%20of%20proteolysis%20in%20glycocalyx%20shedding.%22%2C%22date%22%3A%222017%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.pone.0173217%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221932-6203%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22V9I2F6IG%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A34Z%22%7D%7D%2C%7B%22key%22%3A%227E34WM8U%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Rawat%20et%20al.%22%2C%22parsedDate%22%3A%222017%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BRawat%2C%20A.%3B%20Brejskova%2C%20L.%3B%20Hala%2C%20M.%3B%20Cvrckova%2C%20F.%3B%20Zarsky%2C%20V.%20%26lt%3Bb%26gt%3BThe%20Physcomitrella%20Patens%20Exocyst%20Subunit%20EXO70.3d%20Has%20Distinct%20Roles%20in%20Growth%20and%20Development%2C%20and%20Is%20Essential%20for%20Completion%20of%20the%20Moss%20Life%20Cycle%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BNEW%20PHYTOLOGIST%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2017%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B216%26lt%3B%5C%2Fi%26gt%3B%20%282%2C%20SI%29%2C%20438%26%23x2013%3B454.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fnph.14548%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fnph.14548%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20Physcomitrella%20patens%20exocyst%20subunit%20EXO70.3d%20has%20distinct%20roles%20in%20growth%20and%20development%2C%20and%20is%20essential%20for%20completion%20of%20the%20moss%20life%20cycle%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anamika%22%2C%22lastName%22%3A%22Rawat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucie%22%2C%22lastName%22%3A%22Brejskova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michal%22%2C%22lastName%22%3A%22Hala%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fatima%22%2C%22lastName%22%3A%22Cvrckova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Viktor%22%2C%22lastName%22%3A%22Zarsky%22%7D%5D%2C%22abstractNote%22%3A%22The%20exocyst%2C%20an%20evolutionarily%20conserved%20secretory%20vesicle-tethering%20complex%2C%20spatially%20controls%20exocytosis%20and%20membrane%20turnover%20in%20fungi%2C%20metazoans%20and%20plants.%20The%20exocyst%20subunit%20EXO70%20exists%20in%20multiple%20paralogs%20in%20land%20plants%2C%20forming%20three%20conserved%20clades%20with%20assumed%20distinct%20roles.%20Here%20we%20report%20functional%20analysis%20of%20the%20first%20moss%20exocyst%20subunit%20to%20be%20studied%2C%20Physcomitrella%20patens%20PpEXO70.3d%20%28Pp1s97_91V6%29%2C%20from%20the%2C%20as%20yet%2C%20poorly%20characterized%20EXO70.3%20clade.%20Following%20phylogenetic%20analysis%20to%20confirm%20the%20presence%20of%20three%20ancestral%20land%20plant%20EXO70%20clades%20outside%20angiosperms%2C%20we%20prepared%20and%20phenotypically%20characterized%20loss-of-function%20Ppexo70.3d%20mutants%20and%20localized%20PpEXO70.3d%20in%20vivo%20using%20green%20fluorescent%20protein-tagged%20protein%20expression.%20Disruption%20of%20PpEXO70.3d%20caused%20pleiotropic%20cell%20elongation%20and%20differentiation%20defects%20in%20protonemata%2C%20altered%20response%20towards%20exogenous%20auxin%2C%20increased%20endogenous%20IAA%20concentrations%2C%20along%20with%20defects%20in%20bud%20and%20gametophore%20development.%20During%20mid-archegonia%20development%2C%20an%20abnormal%20egg%20cell%20is%20formed%20and%20subsequently%20collapses%2C%20resulting%20in%20mutant%20sterility.%20Mutants%20exhibited%20altered%20cell%20wall%20and%20cuticle%20deposition%2C%20as%20well%20as%20compromised%20cytokinesis%2C%20consistent%20with%20the%20protein%20localization%20to%20the%20cell%20plate.%20Despite%20some%20functional%20redundancy%20allowing%20survival%20of%20moss%20lacking%20PpEXO70.3d%2C%20this%20subunit%20has%20an%20essential%20role%20in%20the%20moss%20life%20cycle%2C%20indicating%20sub-functionalization%20within%20the%20moss%20EXO70%20family.%22%2C%22date%22%3A%222017%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2Fnph.14548%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220028-646X%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22V9I2F6IG%22%5D%2C%22dateModified%22%3A%222025-11-07T09%3A33%3A01Z%22%7D%7D%2C%7B%22key%22%3A%22YY4V4XKP%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hodek%20et%20al.%22%2C%22parsedDate%22%3A%222017%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BHodek%2C%20O.%3B%20Krizek%2C%20T.%3B%20Coufal%2C%20P.%3B%20Ryslava%2C%20H.%20%26lt%3Bb%26gt%3BDesign%20of%20Experiments%20for%20Amino%20Acid%20Extraction%20from%20Tobacco%20Leaves%20and%20Their%20Subsequent%20Determination%20by%20Capillary%20Zone%20Electrophoresis%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BANALYTICAL%20AND%20BIOANALYTICAL%20CHEMISTRY%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2017%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B409%26lt%3B%5C%2Fi%26gt%3B%20%289%29%2C%202383%26%23x2013%3B2391.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs00216-017-0184-2%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs00216-017-0184-2%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Design%20of%20experiments%20for%20amino%20acid%20extraction%20from%20tobacco%20leaves%20and%20their%20subsequent%20determination%20by%20capillary%20zone%20electrophoresis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ondrej%22%2C%22lastName%22%3A%22Hodek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tomas%22%2C%22lastName%22%3A%22Krizek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pavel%22%2C%22lastName%22%3A%22Coufal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Helena%22%2C%22lastName%22%3A%22Ryslava%22%7D%5D%2C%22abstractNote%22%3A%22In%20this%20study%2C%20we%20optimized%20a%20method%20for%20the%20determination%20of%20free%20amino%20acids%20in%20Nicotiana%20tabacum%20leaves.%20Capillary%20electrophoresis%20with%20contactless%20conductivity%20detector%20was%20used%20for%20the%20separation%20of%2020%20proteinogenic%20amino%20acids%20in%20acidic%20background%20electrolyte.%20Subsequently%2C%20the%20conditions%20of%20extraction%20with%20HCl%20were%20optimized%20for%20the%20highest%20extraction%20yield%20of%20the%20amino%20acids%20because%20sample%20treatment%20of%20plant%20materials%20brings%20some%20specific%20challenges.%20Central%20composite%20face-centered%20design%20with%20fractional%20factorial%20design%20was%20used%20in%20order%20to%20evaluate%20the%20significance%20of%20selected%20factors%20%28HCl%20volume%2C%20HCl%20concentration%2C%20sonication%2C%20shaking%29%20on%20the%20extraction%20process.%20In%20addition%2C%20the%20composite%20design%20helped%20us%20to%20find%20the%20optimal%20values%20for%20each%20factor%20using%20the%20response%20surface%20method.%20The%20limits%20of%20detection%20and%20limits%20of%20quantification%20for%20the%2020%20proteinogenic%20amino%20acids%20were%20found%20to%20be%20in%20the%20order%20of%2010%28-5%29%20and%2010%28-4%29%20mol%20l%28-1%29%2C%20respectively.%20Addition%20of%20acetonitrile%20to%20the%20sample%20was%20tested%20as%20a%20method%20commonly%20used%20to%20decrease%20limits%20of%20detection.%20Ambiguous%20results%20of%20this%20experiment%20pointed%20out%20some%20features%20of%20plant%20extract%20samples%2C%20which%20often%20required%20specific%20approaches.%20Suitability%20of%20the%20method%20for%20metabolomic%20studies%20was%20tested%20by%20analysis%20of%20a%20real%20sample%2C%20in%20which%20all%20amino%20acids%2C%20except%20for%20L-methionine%20and%20L-cysteine%2C%20were%20successfully%20detected.%20The%20optimized%20extraction%20process%20together%20with%20the%20capillary%20electrophoresis%20method%20can%20be%20used%20for%20the%20determination%20of%20proteinogenic%20amino%20acids%20in%20plant%20materials.%20The%20resulting%20inexpensive%2C%20simple%2C%20and%20robust%20method%20is%20well%20suited%20for%20various%20metabolomic%20studies%20in%20plants.%20As%20such%2C%20the%20method%20represents%20a%20valuable%20tool%20for%20research%20and%20practical%20application%20in%20the%20fields%20of%20biology%2C%20biochemistry%2C%20and%20agriculture.%22%2C%22date%22%3A%222017%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1007%5C%2Fs00216-017-0184-2%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221618-2642%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22V9I2F6IG%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A34Z%22%7D%7D%2C%7B%22key%22%3A%22NVD3ULRS%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Sabol%20et%20al.%22%2C%22parsedDate%22%3A%222017%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BSabol%2C%20P.%3B%20Kulich%2C%20I.%3B%20Zarsky%2C%20V.%20%26lt%3Bb%26gt%3BRIN4%20Recruits%20the%20Exocyst%20Subunit%20EXO70B1%20to%20the%20Plasma%20Membrane%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BJOURNAL%20OF%20EXPERIMENTAL%20BOTANY%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2017%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B68%26lt%3B%5C%2Fi%26gt%3B%20%2812%2C%20SI%29%2C%203253%26%23x2013%3B3265.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fjxb%5C%2Ferx007%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fjxb%5C%2Ferx007%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22RIN4%20recruits%20the%20exocyst%20subunit%20EXO70B1%20to%20the%20plasma%20membrane%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Sabol%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ivan%22%2C%22lastName%22%3A%22Kulich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Viktor%22%2C%22lastName%22%3A%22Zarsky%22%7D%5D%2C%22abstractNote%22%3A%22The%20exocyst%20is%20a%20conserved%20vesicle-tethering%20complex%20with%20principal%20roles%20in%20cell%20polarity%20and%20morphogenesis.%20Several%20studies%20point%20to%20its%20involvement%20in%20polarized%20secretion%20during%20microbial%20pathogen%20defense.%20In%20this%20context%2C%20we%20have%20found%20an%20interaction%20between%20the%20Arabidopsis%20EXO70B1%20exocyst%20subunit%2C%20a%20protein%20which%20was%20previously%20associated%20with%20both%20the%20defense%20response%20and%20autophagy%2C%20and%20RPM1%20INTERACTING%20PROTEIN%204%20%28%20RIN4%29%2C%20the%20best%20studied%20member%20of%20the%20NOI%20protein%20family%20and%20a%20known%20regulator%20of%20plant%20defense%20pathways.%20Interestingly%2C%20fragments%20of%20RIN4%20mimicking%20the%20cleavage%20caused%20by%20the%20Pseudomonas%20syringae%20effector%20protease%2C%20AvrRpt2%2C%20fail%20to%20interact%20strongly%20with%20EXO70B1.%20We%20observed%20that%20transiently%20expressed%20RIN4%2C%20but%20not%20the%20plasma%20membrane%20%28%20PM%29%20protein%20aquaporin%20PIP2%2C%20recruits%20EXO70B1%20to%20the%20PM.%20Unlike%20EXO70B1%2C%20RIN4%20does%20not%20recruit%20the%20core%20exocyst%20subunit%20SEC6%20to%20the%20PM%20under%20these%20conditions.%20Furthermore%2C%20the%20AvrRpt2%20effector%20protease%20delivered%20by%20P.%20syringae%20is%20able%20to%20release%20both%20RIN4%20and%20EXO70B1%20to%20the%20cytoplasm.%20We%20present%20a%20model%20for%20how%20RIN4%20might%20regulate%20the%20localization%20and%20putative%20function%20of%20EXO70B1%20and%20speculate%20on%20the%20role%20the%20AvrRpt2%20protease%20might%20have%20in%20the%20regulation%20of%20this%20defense%20response.%22%2C%22date%22%3A%222017%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fjxb%5C%2Ferx007%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220022-0957%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22V9I2F6IG%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A34Z%22%7D%7D%2C%7B%22key%22%3A%22MFK66P9D%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Bauerova%20et%20al.%22%2C%22parsedDate%22%3A%222017%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BBauerova%2C%20P.%3B%20Vinklerova%2C%20J.%3B%20Hranicek%2C%20J.%3B%20Corba%2C%20V.%3B%20Vojtek%2C%20L.%3B%20Svobodova%2C%20J.%3B%20Vinkler%2C%20M.%20%26lt%3Bb%26gt%3BAssociations%20of%20Urban%20Environmental%20Pollution%20with%20Health-Related%20Physiological%20Traits%20in%20a%20Free-Living%20Bird%20Species%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BSCIENCE%20OF%20THE%20TOTAL%20ENVIRONMENT%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2017%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B601%26lt%3B%5C%2Fi%26gt%3B%2C%201556%26%23x2013%3B1565.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.scitotenv.2017.05.276%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.scitotenv.2017.05.276%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Associations%20of%20urban%20environmental%20pollution%20with%20health-related%20physiological%20traits%20in%20a%20free-living%20bird%20species%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petra%22%2C%22lastName%22%3A%22Bauerova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jitka%22%2C%22lastName%22%3A%22Vinklerova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jakub%22%2C%22lastName%22%3A%22Hranicek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vojtech%22%2C%22lastName%22%3A%22Corba%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Libor%22%2C%22lastName%22%3A%22Vojtek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jana%22%2C%22lastName%22%3A%22Svobodova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michal%22%2C%22lastName%22%3A%22Vinkler%22%7D%5D%2C%22abstractNote%22%3A%22Urban%20environmental%20pollution%20results%20in%20contamination%20of%20the%20tissues%20of%20synanthropic%20organisms%20by%20toxic%20trace%20elements%20with%20potential%20impacts%20on%20human%20health.%20Passerine%20birds%20may%20serve%20as%20convenient%20indicators%20of%20such%20contamination.%20In%20this%20study%20we%20investigated%20the%20effect%20of%20blood%20and%20plumage%20contamination%20with%20heavy%20metals%20%28lead%20Pb%2C%20cadmium%20Cd%2C%20copper%20Cu%2C%20chromium%20Cr%29%20and%20arsenic%20metalloid%20%28As%29%20on%20condition%2C%20health%20and%20ornamental%20colour%20in%20free-living%20great%20tit%20%28Parus%20major%29%20males%20from%2013%20cities%20across%20the%20Czech%20Republic%20%28EU%29%2C%20mist%20netted%20during%20the%20early%20breading%20season%20%28April-May%29.%20Our%20results%20showed%20a%20significant%20association%20of%20heavy%20metal%20tissue%20contamination%20with%20immune%20function%2C%20namely%20leukocyte%20composition%20in%20the%20avian%20blood%20circulation.%20High%20heavy%20metal%20contamination%20in%20bird%20feathers%20was%20linked%20to%20a%20high%20heterophil%5C%2Flymphocyte%20%28H%5C%2FL%29%20ratio%2C%20indicating%20long-term%20stress%20in%20individuals%20inhabiting%20heavily%20polluted%20environments.%20In%20contrast%2C%20males%20with%20higher%20concentrations%20of%20heavy%20metals%20in%20blood%20had%20a%20lower%20H%5C%2FL%20ratio%2C%20assumingly%20due%20to%20the%20direct%20toxicity%20of%20heavy%20metals%20in%20certain%20cell%20types.%20This%20is%20also%20supported%20by%20traits%20indicative%20of%20anaemia-like%20haemolytic%20conditions%20%28decreased%20absolute%20erythrocyte%20count%29%20and%20increased%20haematopoiesis%20%28a%20tendency%20for%20increased%20frequencies%20of%20immature%20erythrocytes%29.%20We%20did%20not%20find%20any%20association%20of%20heavy%20metal%20contamination%20with%20the%20bacteriolytic%20activity%20of%20plasma%20complement%2C%20feather%20growth%20or%20ornamentation%20%28black%20breast%20stripe%20area%20and%20yellow%20colouration%29.%20There%20was%20no%20significant%20relationship%20between%20heavy%20metal%20contamination%20in%20blood%20or%20feathers%20and%20PM10%20pollution%20at%20the%20study%20sites.%20Our%20correlational%20study%20is%20the%20first%20to%20show%20on%20a%20large%20geographic%20scale%20that%20despite%20strict%20European%20air%20pollution%20regulations%20and%20regular%20monitoring%20that%20have%20allowed%20general%20improvements%20in%20atmospheric%20contamination%2C%20non-degradable%20heavy%20metals%20persistently%20contaminate%20animal%20blood%20and%20feathers%20in%20anthropogenic%20environments%20at%20levels%20thatmay%20have%20subclinical%20yet%20physiological%20effects%20with%20varied%20influence%20on%20health.%20%28C%29%202017%20Elsevier%20B.V.%20All%20rights%20reserved.%22%2C%22date%22%3A%222017%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.scitotenv.2017.05.276%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220048-9697%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22V9I2F6IG%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A34Z%22%7D%7D%2C%7B%22key%22%3A%22JYZ9ZA9H%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Kolar%20et%20al.%22%2C%22parsedDate%22%3A%222017%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BKolar%2C%20D.%3B%20Gresikova%2C%20M.%3B%20Waskova-Arnostova%2C%20P.%3B%20Elsnicova%2C%20B.%3B%20Kohutova%2C%20J.%3B%20Hornikova%2C%20D.%3B%20Vebr%2C%20P.%3B%20Neckar%2C%20J.%3B%20Blahova%2C%20T.%3B%20Kasparova%2C%20D.%3B%20Novotny%2C%20J.%3B%20Kolar%2C%20F.%3B%20Novakova%2C%20O.%3B%20Zurmanova%2C%20J.%20M.%20%26lt%3Bb%26gt%3BAdaptation%20to%20Chronic%20Continuous%20Hypoxia%20Potentiates%20Akt%5C%2FHK2%20Anti-Apoptotic%20Pathway%20during%20Brief%20Myocardial%20Ischemia%5C%2FReperfusion%20Insult%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BMOLECULAR%20AND%20CELLULAR%20BIOCHEMISTRY%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2017%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B432%26lt%3B%5C%2Fi%26gt%3B%20%281%26%23x2013%3B2%29%2C%2099%26%23x2013%3B108.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs11010-017-3001-5%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs11010-017-3001-5%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Adaptation%20to%20chronic%20continuous%20hypoxia%20potentiates%20Akt%5C%2FHK2%20anti-apoptotic%20pathway%20during%20brief%20myocardial%20ischemia%5C%2Freperfusion%20insult%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Kolar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Milada%22%2C%22lastName%22%3A%22Gresikova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petra%22%2C%22lastName%22%3A%22Waskova-Arnostova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Barbara%22%2C%22lastName%22%3A%22Elsnicova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jana%22%2C%22lastName%22%3A%22Kohutova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniela%22%2C%22lastName%22%3A%22Hornikova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pavel%22%2C%22lastName%22%3A%22Vebr%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jan%22%2C%22lastName%22%3A%22Neckar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tereza%22%2C%22lastName%22%3A%22Blahova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dita%22%2C%22lastName%22%3A%22Kasparova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jiri%22%2C%22lastName%22%3A%22Novotny%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Frantisek%22%2C%22lastName%22%3A%22Kolar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olga%22%2C%22lastName%22%3A%22Novakova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jitka%20M.%22%2C%22lastName%22%3A%22Zurmanova%22%7D%5D%2C%22abstractNote%22%3A%22Adaptation%20to%20chronic%20hypoxia%20represents%20a%20potential%20cardioprotective%20intervention%20reducing%20the%20extent%20of%20acute%20ischemia%5C%2Freperfusion%20%28I%5C%2FR%29%20injury%2C%20which%20is%20a%20major%20cause%20of%20death%20worldwide.%20The%20main%20objective%20of%20this%20study%20was%20to%20investigate%20the%20anti-apoptotic%20Akt%5C%2Fhexokinase%202%20%28HK2%29%20pathway%20in%20hypoxic%20hearts%20subjected%20to%20I%5C%2FR%20insult.%20Hearts%20isolated%20from%20male%20Wistar%20rats%20exposed%20either%20to%20continuous%20normobaric%20hypoxia%20%28CNH%3B%2010%25%20O-2%29%20or%20to%20room%20air%20for%203%20weeks%20were%20perfused%20according%20to%20Langendorff%20and%20subjected%20to%2010%20min%20of%20no-flow%20ischemia%20and%2010%20min%20of%20reperfusion.%20The%20hearts%20were%20collected%20either%20after%20ischemia%20or%20after%20reperfusion%20and%20used%20for%20protein%20analyses%20and%20quantitative%20fluorescence%20microscopy.%20The%20CNH%20resulted%20in%20increased%20levels%20of%20HK1%20and%20HK2%20proteins%20and%20the%20total%20HK%20activity%20after%20ischemia%20compared%20to%20corresponding%20normoxic%20group.%20Similarly%2C%20CNH%20hearts%20exhibited%20increased%20ischemic%20level%20of%20Akt%20protein%20phosphorylated%20on%20Ser%28473%29.%20The%20CNH%20also%20strengthened%20the%20interaction%20of%20HK2%20with%20mitochondria%20and%20prevented%20downregulation%20of%20mitochondrial%20creatine%20kinase%20after%20reperfusion.%20The%20Bax%5C%2FBcl-2%20ratio%20was%20significantly%20lower%20after%20I%5C%2FR%20in%20CNH%20hearts%20than%20in%20normoxic%20ones%2C%20suggesting%20a%20lower%20probability%20of%20apoptosis.%20In%20conclusion%2C%20the%20Akt%5C%2FHK2%20pathway%20is%20likely%20to%20play%20a%20role%20in%20the%20development%20of%20a%20cardioprotective%20phenotype%20of%20CNH%20by%20preventing%20the%20detachment%20of%20HK2%20from%20mitochondria%20at%20reperfusion%20period%20and%20decreases%20the%20Bax%5C%2FBcl-2%20ratio%20during%20I%5C%2FR%20insult%2C%20thereby%20lowering%20the%20probability%20of%20apoptosis%20activation%20in%20the%20mitochondrial%20compartment.%22%2C%22date%22%3A%222017%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1007%5C%2Fs11010-017-3001-5%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%220300-8177%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22V9I2F6IG%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A34Z%22%7D%7D%2C%7B%22key%22%3A%22QGBKL5EZ%22%2C%22library%22%3A%7B%22id%22%3A5891878%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Suchanova%20et%20al.%22%2C%22parsedDate%22%3A%222017%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%20style%3D%26quot%3Bclear%3A%20left%3B%20%26quot%3B%26gt%3B%5Cn%20%20%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-left-margin%26quot%3B%20style%3D%26quot%3Bfloat%3A%20left%3B%20padding-right%3A%200.5em%3B%20text-align%3A%20right%3B%20width%3A%201em%3B%26quot%3B%26gt%3B1.%26lt%3B%5C%2Fdiv%26gt%3B%26lt%3Bdiv%20class%3D%26quot%3Bcsl-right-inline%26quot%3B%20style%3D%26quot%3Bmargin%3A%200%20.4em%200%201.5em%3B%26quot%3B%26gt%3BSuchanova%2C%20J.%20Z.%3B%20Neburkova%2C%20J.%3B%20Spanielova%2C%20H.%3B%20Forstova%2C%20J.%3B%20Cigler%2C%20P.%20%26lt%3Bb%26gt%3BRetargeting%20Polyomavirus-Like%20Particles%20to%20Cancer%20Cells%20by%20Chemical%20Modification%20of%20Capsid%20Surface%26lt%3B%5C%2Fb%26gt%3B.%20%26lt%3Bi%26gt%3BBIOCONJUGATE%20CHEMISTRY%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bb%26gt%3B2017%26lt%3B%5C%2Fb%26gt%3B%2C%20%26lt%3Bi%26gt%3B28%26lt%3B%5C%2Fi%26gt%3B%20%282%29%2C%20307%26%23x2013%3B313.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.bioconjchem.6b00622%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.bioconjchem.6b00622%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%20%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Retargeting%20Polyomavirus-Like%20Particles%20to%20Cancer%20Cells%20by%20Chemical%20Modification%20of%20Capsid%20Surface%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jirina%20Zackova%22%2C%22lastName%22%3A%22Suchanova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jitka%22%2C%22lastName%22%3A%22Neburkova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hana%22%2C%22lastName%22%3A%22Spanielova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jitka%22%2C%22lastName%22%3A%22Forstova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petr%22%2C%22lastName%22%3A%22Cigler%22%7D%5D%2C%22abstractNote%22%3A%22Virus-like%20particles%20based%20on%20polyomaviruses%20%28PVLPs%29%20are%20promising%20delivery%20devices%20for%20various%20cargoes%2C%20including%20nucleic%20acids%2C%20imaging%20probes%2C%20and%20therapeutic%20agents.%20In%20biological%20environments%2C%20the%20major%20coat%20protein%20VP1%20interacts%20with%20ubiquitously%20distributed%20sialic%20acid%20residues%2C%20and%20therefore%20PVLPs%20show%20a%20broad%20tropism.%20For%20selective%20targeting%2C%20appropriate%20engineering%20of%20the%20PVLP%20surface%20is%20needed.%20Here%2C%20we%20describe%20a%20chemical%20approach%20to%20retarget%20PVLPs%20to%20cancer%20cells%20displaying%20abnormally%20high%20levels%20of%20transferrin%20receptor.%20We%20created%20an%20array%20of%20transferrin%20molecules%20on%20the%20surface%20of%20PVLPs%20by%20combining%20a%20high-yielding%20bioconjugation%20approach%20with%20specific%20point%20modification%20of%20transferrin.%20This%20artificial%20surface%20protein%20architecture%20enables%20%28i%29%20suppression%20of%20natural%20VP1-specific%20interactions%20by%20blocking%20the%20surface%20conformational%20epitope%20on%20the%20VP1%20protein%2C%20%28ii%29%20unusually%20high%20cellular%20uptake%20efficiency%2C%20and%20%28iii%29%20selective%20retargeting%20of%20PVLPs%20to%20osteosarcoma%20%28U2OS%29%20and%20lymphoblastoid%20leukemia%20%28CCRF-CEM%29%20cells.%22%2C%22date%22%3A%222017%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1021%5C%2Facs.bioconjchem.6b00622%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%22%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221043-1802%22%2C%22language%22%3A%22%22%2C%22collections%22%3A%5B%22V9I2F6IG%22%5D%2C%22dateModified%22%3A%222025-03-19T11%3A58%3A34Z%22%7D%7D%5D%7D
1.
Bourland, W.; Rotterova, J.; Cepicka, I. Morphologic and Molecular Characterization of Seven Species of the Remarkably Diverse and Widely Distributed Metopid Genus Urostomides Jankowski, 1964 (Armophorea, Ciliophora). Eur. J. Protistol. 2017, 61, 194–232. https://doi.org/10.1016/j.ejop.2017.07.003.
1.
Vavra, J.; Hylis, M.; Fiala, I.; Sacherova, V.; Vossbrinck, C. R. Microsporidian Genus Berwaldia (Opisthosporidia, Microsporidia), Infecting Daphnids (Crustacea, Branchiopoda): Biology, Structure, Molecular Phylogeny and Description of Two New Species. Eur. J. Protistol. 2017, 61, 1–12. https://doi.org/10.1016/j.ejop.2017.07.005.
1.
Sklenar, F.; Jurjevic, Z.; Zalar, P.; Frisvad, J. C.; Visagie, C. M.; Kolarik, M.; Houbraken, J.; Chen, A. J.; Yilmaz, N.; Seifert, K. A.; Coton, M.; Deniel, F.; Gunde-Cimerman, N.; Samson, R. A.; Peterson, S. W.; Hubka, V. Phylogeny of Xerophilic Aspergilli (Subgenus Aspergillus) and Taxonomic Revision of Section Restricti. Stud. Mycol. 2017, No. 88, 161–236. https://doi.org/10.1016/j.simyco.2017.09.002.
1.
Chen, A. J.; Hubka, V.; Frisvad, J. C.; Visagie, C. M.; Houbraken, J.; Meijer, M.; Varga, J.; Demirel, R.; Jurjevic, Z.; Kubatova, A.; Sklenar, F.; Zhou, Y. G.; Samson, R. A. Polyphasic Taxonomy of Aspergillus Section Aspergillus (Formerly Eurotium), and Its Occurrence in Indoor Environments and Food. Stud. Mycol. 2017, No. 88, 37–135. https://doi.org/10.1016/j.simyco.2017.07.001.
1.
Pilarska, D.; Takov, D.; Hyliš, M.; Radek, R.; Fiala, I.; Solter, L.; Linde, A. Natural Occurrence of Microsporidia Infecting Lepidoptera in Bulgaria. Acta Parasitologica 2017, 62 (4). https://doi.org/10.1515/ap-2017-0104.
1.
Rimnacova, J.; Mikes, L.; Turjanicova, L.; Bulantova, J.; Horak, P. Changes in Surface Glycosylation and Glycocalyx Shedding in Trichobilharzia Regenti (Schistosomatidae) during the Transformation of Cercaria to Schistosomulum. PLOS ONE 2017, 12 (3). https://doi.org/10.1371/journal.pone.0173217.
1.
Rawat, A.; Brejskova, L.; Hala, M.; Cvrckova, F.; Zarsky, V. The Physcomitrella Patens Exocyst Subunit EXO70.3d Has Distinct Roles in Growth and Development, and Is Essential for Completion of the Moss Life Cycle. NEW PHYTOLOGIST 2017, 216 (2, SI), 438–454. https://doi.org/10.1111/nph.14548.
1.
Hodek, O.; Krizek, T.; Coufal, P.; Ryslava, H. Design of Experiments for Amino Acid Extraction from Tobacco Leaves and Their Subsequent Determination by Capillary Zone Electrophoresis. ANALYTICAL AND BIOANALYTICAL CHEMISTRY 2017, 409 (9), 2383–2391. https://doi.org/10.1007/s00216-017-0184-2.
1.
Sabol, P.; Kulich, I.; Zarsky, V. RIN4 Recruits the Exocyst Subunit EXO70B1 to the Plasma Membrane. JOURNAL OF EXPERIMENTAL BOTANY 2017, 68 (12, SI), 3253–3265. https://doi.org/10.1093/jxb/erx007.
1.
Bauerova, P.; Vinklerova, J.; Hranicek, J.; Corba, V.; Vojtek, L.; Svobodova, J.; Vinkler, M. Associations of Urban Environmental Pollution with Health-Related Physiological Traits in a Free-Living Bird Species. SCIENCE OF THE TOTAL ENVIRONMENT 2017, 601, 1556–1565. https://doi.org/10.1016/j.scitotenv.2017.05.276.
1.
Kolar, D.; Gresikova, M.; Waskova-Arnostova, P.; Elsnicova, B.; Kohutova, J.; Hornikova, D.; Vebr, P.; Neckar, J.; Blahova, T.; Kasparova, D.; Novotny, J.; Kolar, F.; Novakova, O.; Zurmanova, J. M. Adaptation to Chronic Continuous Hypoxia Potentiates Akt/HK2 Anti-Apoptotic Pathway during Brief Myocardial Ischemia/Reperfusion Insult. MOLECULAR AND CELLULAR BIOCHEMISTRY 2017, 432 (1–2), 99–108. https://doi.org/10.1007/s11010-017-3001-5.
1.
Suchanova, J. Z.; Neburkova, J.; Spanielova, H.; Forstova, J.; Cigler, P. Retargeting Polyomavirus-Like Particles to Cancer Cells by Chemical Modification of Capsid Surface. BIOCONJUGATE CHEMISTRY 2017, 28 (2), 307–313. https://doi.org/10.1021/acs.bioconjchem.6b00622.
2006 – 2016
5891878
GE86BF5K
1
https://raw.githubusercontent.com/Schebique/vmcf-konfmi/refs/heads/main/vmcf-web-style.csl
50
date
desc
4983
https://web.natur.cuni.cz/sekce-bi/VMCF/wp-content/plugins/zotpress/
statussuccessupdateneededfalseinstancefalsemetarequest_last50request_next50used_cachetruedatakeyV26AU6VDlibraryid5891878metacreatorSummaryVavraetal.parsedDate2016-03numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtVavraJ.HylisM.FialaI.NebesarovaJ.ltbgtGlobulisporaMitoportansn.g.n.Sp.OpisthosporidiaMicrosporidiaaMicrosporidianParasiteofDaphnidswithUnusualSporeOrganizationandProminentMitosome-likeVesiclesltbgt.ltigtJ.Invertebr.Pathol.ltigtltbgt2016ltbgtltigt135ltigt43x201352.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.jip.2016.02.003039gthttpsdoi.org10.1016j.jip.2016.02.003ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleGlobulisporamitoportansn.g.n.sp.OpisthosporidiaMicrosporidiaamicrosporidianparasiteofdaphnidswithunusualsporeorganizationandprominentmitosome-likevesiclescreatorscreatorTypeauthorfirstNameJirilastNameVavracreatorTypeauthorfirstNameMiroslavlastNameHyliscreatorTypeauthorfirstNameIvanlastNameFialacreatorTypeauthorfirstNameJanalastNameNebesarovaabstractNoteThemicrosporidianparasiteGlobulisporamitoportansn.g.n.sp.infectstheintestinalepitheliumoftwospeciesofdaphnidsCrustaceaCladocera.Maturesporesarethin-walledandpossessanoveltypeofpolaroplastwithaconspicuouspartconsistingofglobulesthatoccupiesalargepartofthesporevolume.Bothdevelopmentalstagesandthesporespossesslargeelectron-lucentvesiclesenvelopedbyadoublemembraneandfilledwithaninternalweboffilamentousmaterialcorrespondingstructurallytomicrosporidianmitosomes.TheSSUrRNAphylogenyplacesGlobulisporaintoaspecificquotEnterocytospora-likequotGladepartofalargequotnon-enterocytozoonidaequotGladegroupingaheterogenousassemblageofmicrosporidiainfectingalmostexclusivelyinsectsandcrustacea.C2016ElsevierInc.Allrightsreserved.dateMAR2016sectionpartNumberpartTitleDOI10.1016j.jip.2016.02.003citationKeyurlPMIDPMCIDISSN0022-20111096-0805languageEnglishcollectionsGE86BF5KdateModified2025-11-07T094522Zkey2TBKE9EJlibraryid5891878metacreatorSummaryVavraetal.parsedDate2016numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtVavraJ.HylisM.FialaI.RefardtD.LarssonJ.I.R.ltbgtMicrosporidiainaWoodlandPoolI.LanatosporaCostataSpn.OpisthosporidiaMicrosporidiaParasiteofMegacyclopsViridisCrustaceaCopepodaFineStructureandMolecularPhylogenyltbgt.ltigtActaProtozool.ltigtltbgt2016ltbgtltigt55ltigt4269x2013280.ltaclass039zp-DOIURL039href039httpsdoi.org10.446716890027AP.16.023.6010039gthttpsdoi.org10.446716890027AP.16.023.6010ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMicrosporidiainaWoodlandPoolI.Lanatosporacostataspn.OpisthosporidiaMicrosporidiaParasiteofMegacyclopsviridisCrustaceaCopepodaFineStructureandMolecularPhylogenycreatorscreatorTypeauthorfirstNameJirilastNameVavracreatorTypeauthorfirstNameMiroslavlastNameHyliscreatorTypeauthorfirstNameIvanlastNameFialacreatorTypeauthorfirstNameDominiklastNameRefardtcreatorTypeauthorfirstNameJ.I.RonnylastNameLarssonabstractNoteLanatosporacostatasp.n.anewmicrosporidianparasiteoftheadiposeandconnectivetissuecellsofMegacyclopsviridisJurine1820CopepodaCyclopidaeisdescribed.Itwasisolatedfromawoodlandpoolwitharichanddiversefaunaofmicrosporidia-infectedsmallcrustaceansandinsectlarvae.TheparasiteisstructurallysimilartothegenusLanatosporaVoronin1989characterizedbyacomplexdecoratedexospore.Inthedescribedspeciestheexosporeisarmouredbyaconspicuouslayerofinterwovenribsforminglabyrinthonthesporesurface.SSUrRNAphylogenyplacestheorganisminthequotaquaticoutgroupquotofmicrosporidiaVossbrincketal.2004whichincludesanumberofmicrosporidiafromaquaticmicrocrustaceaoftheclassesCopepodaandCladocera.TheincreasingnumberofavailableSSUrRNAgenesequencesofthesemicrosporidiaallowsamoredetailedinterpretationoftheirdevelopmentalhistories.date2016sectionpartNumberpartTitleDOI10.446716890027AP.16.023.6010citationKeyurlPMIDPMCIDISSN0065-15831689-0027languageEnglishcollectionsGE86BF5KdateModified2025-11-07T093417ZkeyGZU93E96libraryid5891878metacreatorSummaryRohanovaetal.parsedDate2016numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtRohanovaM.SchaeferC.W.KrizkovaP.VilimovaJ.ltbgtScentEfferentSystemofDorsalAbdominalScentGlandsinNymphsofRhopalidaeHemipteraHeteropteraPentatomomorphaandItsComparisonwithOtherPentatomomorphaltbgt.ltigtZool.Anz.ltigtltbgt2016ltbgtltigt260ltigt1x201310.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.jcz.2015.11.001039gthttpsdoi.org10.1016j.jcz.2015.11.001ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleScentefferentsystemofdorsalabdominalscentglandsinnymphsofRhopalidaeHemipteraHeteropteraPentatomomorphaanditscomparisonwithotherPentatomomorphacreatorscreatorTypeauthorfirstNameMarketalastNameRohanovacreatorTypeauthorfirstNameCarlW.lastNameSchaefercreatorTypeauthorfirstNamePetralastNameKrizkovacreatorTypeauthorfirstNameJitkalastNameVilimovaabstractNoteThescentefferentsystemofthenymphalabdominalscentglandsoftheRhopalidaeRhopalinaeHeteropterafromtwotribesRhopaliniRhopalusAeschyntelusmaculatusFieber1837andChorosominiChorosomaschillingiSchilling1829weredescribedforthefirsttimeusingSEM.Twounpairedostiolesonmedialscleritesareconnectedbyoneevaporativeareabearingtwopatternsofmicrosculpturetooth-shapedandcone-shaped.AlsoforthefirsttimethescentefferentsystemofdorsalabdominalscentglandsofCoreusmarginatusLinnaeus1758HeteropteraCoreidaeCoreinaenymphswasdescribed.ThesystemwascomparedwithinthePentatomoideaLygaeoideaCoreidaeandRhopalidaethestateinRhopalidaeisintermediatebetweenLygaeoideaandCoreidae.C2015ElsevierGmbH.Allrightsreserved.date2016sectionpartNumberpartTitleDOI10.1016j.jcz.2015.11.001citationKeyurlPMIDPMCIDISSN0044-5231languageEnglishcollectionsGE86BF5KdateModified2025-11-07T093417ZkeyEFTMWSWClibraryid5891878metacreatorSummaryLankovaetal.parsedDate2016numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtLankovaM.HumpolickovaJ.VosolsobeS.CitZ.LacekJ.CovanM.CovanovaM.HofM.PetrasekJ.ltbgtDeterminationofDynamicsofPlantPlasmaMembraneProteinswithFluorescenceRecoveryandRasterImageCorrelationSpectroscopyltbgt.ltigtMICROSCOPYANDMICROANALYSISltigtltbgt2016ltbgtltigt22ltigt2290x2013299.ltaclass039zp-DOIURL039href039httpsdoi.org10.1017S1431927616000568039gthttpsdoi.org10.1017S1431927616000568ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDeterminationofDynamicsofPlantPlasmaMembraneProteinswithFluorescenceRecoveryandRasterImageCorrelationSpectroscopycreatorscreatorTypeauthorfirstNameMartinalastNameLankovacreatorTypeauthorfirstNameJanalastNameHumpolickovacreatorTypeauthorfirstNameStanislavlastNameVosolsobecreatorTypeauthorfirstNameZdeneklastNameCitcreatorTypeauthorfirstNameJozeflastNameLacekcreatorTypeauthorfirstNameMartinlastNameCovancreatorTypeauthorfirstNameMiladalastNameCovanovacreatorTypeauthorfirstNameMartinlastNameHofcreatorTypeauthorfirstNameJanlastNamePetrasekabstractNoteAnumberoffluorescencemicroscopytechniquesaredescribedtostudydynamicsoffluorescentlylabeledproteinslipidsnucleicacidsandwholeorganelles.HoweverforstudiesofplantplasmamembranePMproteinsthenumberofthesetechniquesisstilllimitedbecauseofthehighcomplexityofprocessesthatdeterminethedynamicsofPMproteinsandtheexistenceofcellwall.HerewereportontheusageofrasterimagecorrelationspectroscopyRICSforstudiesofintegralPMproteinsinsuspension-culturedtobaccocellsandshowitspotentialincomparisonwiththemorewidelyusedfluorescencerecoveryafterphotobleachingmethod.ForRICSasetofmicroscopyimagesisobtainedbysingle-photonconfocallaserscanningmicroscopyCLSM.Fluorescencefluctuationsaresubsequentlycorrelatedbetweenindividualpixelsandtheinformationonproteinmobilityareextractedusingamodelthatconsidersprocessesgeneratingthefluctuationssuchasdiffusionandchemicalbindingreactions.AsweshowhereusinganexampleoftwointegralPMtransportersoftheplanthormoneauxinRICSuncoveredtheirdistinctshort-distancelateralmobilitywithinthePMthatisdependentoncytoskeletonandsterolcompositionofthePM.RICSwhichisroutinelyaccessibleonmodernCLSMinstrumentsthusrepresentsavaluableapproachforstudiesofdynamicsofPMproteinsinplants.date2016sectionpartNumberpartTitleDOI10.1017S1431927616000568citationKeyurlPMIDPMCIDISSN1431-9276languagecollectionsGE86BF5KdateModified2025-03-19T115834ZkeyF6BW8AQKlibraryid5891878metacreatorSummarySmrzetal.parsedDate2016numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtSmrzJ.SoukalovaH.CatskaV.HubertJ.ltbgtFeedingPatternsofTyrophagusPutrescentiaeSarcoptiformesAcaridaeIndicateThatMycophagyIsNotaSingleandHomogeneousCategoryofNutritionalBiologyltbgt.ltigtJOURNALOFINSECTSCIENCEltigtltbgt2016ltbgtltigt16ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1093jisesaiew070039gthttpsdoi.org10.1093jisesaiew070ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleFeedingPatternsofTyrophagusputrescentiaeSarcoptiformesAcaridaeIndicateThatMycophagyIsNotaSingleandHomogeneousCategoryofNutritionalBiologycreatorscreatorTypeauthorfirstNameJaroslavlastNameSmrzcreatorTypeauthorfirstNameHanalastNameSoukalovacreatorTypeauthorfirstNameVlastalastNameCatskacreatorTypeauthorfirstNameJanlastNameHubertabstractNoteMycophagyshouldnotbeconsideredasasingleandhomogeneouscategoryofnutritionalbiologyduetothespecificsymbioticchitinolyticbacteriaassociatedwithmitesandfungi.TotestinteractionamongmitesfungiandchitinolyticbacteriaexperimentswereconductedonthemodelspeciesTyrophagusputrescentiaeSchrank.MucorspAlternariaalternataPenicilliumclaviformeP.griseofulvumandVerticilliumsp.wereplatedontomaltagarandofferedtoT.putrescentiaeinthelaboratory.MiteswereevaluatedutilizingmicroanatomicalexaminationbasedonhistologyexcrementanalysisusingfluorescencemicroscopybacterialplatingimpactofmitehomogenateonfungiinPetridishesreproductionofmitesfeedinguponeachfungusandisolationofassociatedbacteriainsidemites.Therewerecleardifferencesregardingthedigestedsporesofdifferentfungipassingthroughthegutandsubsequentlyinthefeces.Abundancesofbacterialcellsinexcrementalsocorrespondedtothefungioffered.Theextractsfrommiteshadchitinolyticactivityandtheplatedbacteriaareknowntoproduceexochitinases.Thevariousfeedingpatternsobservedwerecausedbydifferencesinthecellwallstructuresofthetestedfungi.Thestudyillustratesthatmycophagyinsaprophagousmitesdoesnotconsistofasinglepatternbutratherthatitcanbeclassifiedintoseveralsub-patternsdependinguponthedigestedfungalspeciesanditsparts.Theresultspointtoanearlysymbioticrelationshipbetweenchitinolyticbacteriaanddigestedfungiinmycophagousmicroarthropods.date2016sectionpartNumberpartTitleDOI10.1093jisesaiew070citationKeyurlPMIDPMCIDISSN1536-2442languagecollectionsGE86BF5KdateModified2025-03-19T115834Zkey448YVYCUlibraryid5891878metacreatorSummaryMicovaetal.parsedDate2016numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMicovaP.HahnovaK.HlavackovaM.ElsnicovaB.ChytilovaA.HolzerovaK.ZurmanovaJ.NeckarJ.KolarF.NovakovaO.NovotnyJ.ltbgtChronicIntermittentHypoxiaAffectstheCytosolicPhospholipaseA2AlphaCyclooxygenase2PathwayviaBeta2-Adrenoceptor-MediatedERKP38Stimulationltbgt.ltigtMOLECULARANDCELLULARBIOCHEMISTRYltigtltbgt2016ltbgtltigt423ltigt1x20132151x2013163.ltaclass039zp-DOIURL039href039httpsdoi.org10.1007s11010-016-2833-8039gthttpsdoi.org10.1007s11010-016-2833-8ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleChronicintermittenthypoxiaaffectsthecytosolicphospholipaseA2alphacyclooxygenase2pathwayviabeta2-adrenoceptor-mediatedERKp38stimulationcreatorscreatorTypeauthorfirstNamePetralastNameMicovacreatorTypeauthorfirstNameKlaralastNameHahnovacreatorTypeauthorfirstNameMarketalastNameHlavackovacreatorTypeauthorfirstNameBarbaralastNameElsnicovacreatorTypeauthorfirstNameAnnalastNameChytilovacreatorTypeauthorfirstNameKristynalastNameHolzerovacreatorTypeauthorfirstNameJitkalastNameZurmanovacreatorTypeauthorfirstNameJanlastNameNeckarcreatorTypeauthorfirstNameFrantiseklastNameKolarcreatorTypeauthorfirstNameOlgalastNameNovakovacreatorTypeauthorfirstNameJirilastNameNovotnyabstractNoteCardiacresistanceagainstacuteischemiareperfusionIRinjurycanbeenhancedbyadaptationtochronicintermittenthypoxiaCIHbutthechangesatthemolecularlevelassociatedwiththisadaptationarestillnotfullyexplored.PhospholipaseA2PLA2playsanimportantroleinphospholipidmetabolismandmaycontributetomembranedestructionunderconditionsofenergydeprivationduringIR.TheaimofthisstudywastodeterminetheeffectofCIH7000m8hday5weeksontheexpressionofcytosolicPLA2alphacPLA2alphaanditsphosphorylatedformp-cPLA2alphaaswellasotherrelatedsignalingproteinsintheleftventricularmyocardiumofadultmaleWistarrats.AdaptationtoCIHincreasedthetotalcontentofcPLA2alphaby14inmyocardialhomogenateandenhancedtheassociationofp-cPLA2alphawiththenuclearmembraneby85.Thetotalnumberofbeta-adrenoceptorsbeta-ARsdidnotchangebutthebeta2beta1ratiomarkedlyincreasedduetotheelevationofbeta2-ARsanddropinbeta1-ARs.Inparalleltheamountofadenylylcyclasedecreasedby49andGialphaproteinsincreasedbyabout50.Besidesthatcyclooxygenase2COX-2andprostaglandinE-2PGE2increasedby36and84respectively.InparallelwedetectedincreasedphosphorylationofproteinkinaseCalphaERK12andp38by1248and19respectively.ThesedatasuggestthatadaptivechangesinducedinthemyocardiumbyCIHmayincludeactivationofcPLA2alphaandCOX-2viabeta2-ARGi-mediatedstimulationoftheERKp38pathway.date2016sectionpartNumberpartTitleDOI10.1007s11010-016-2833-8citationKeyurlPMIDPMCIDISSN0300-8177languagecollectionsGE86BF5KdateModified2025-03-19T115834ZkeyDEL6WQVGlibraryid5891878metacreatorSummaryHahnovaetal.parsedDate2016numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHahnovaK.PacesovaD.VolfovaB.CervenaK.KasparovaD.ZurmanovaJ.BendovaZ.ltbgtCircadianDexras1inRatsDevelopmentLocationandResponsivenesstoLightltbgt.ltigtCHRONOBIOLOGYINTERNATIONALltigtltbgt2016ltbgtltigt33ltigt2141x2013150.ltaclass039zp-DOIURL039href039httpsdoi.org10.310907420528.2015.1120741039gthttpsdoi.org10.310907420528.2015.1120741ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleCircadianDexras1inratsDevelopmentlocationandresponsivenesstolightcreatorscreatorTypeauthorfirstNameKlaralastNameHahnovacreatorTypeauthorfirstNameDominikalastNamePacesovacreatorTypeauthorfirstNameBarboralastNameVolfovacreatorTypeauthorfirstNameKaterinalastNameCervenacreatorTypeauthorfirstNameDitalastNameKasparovacreatorTypeauthorfirstNameJitkalastNameZurmanovacreatorTypeauthorfirstNameZdenkalastNameBendovaabstractNoteDexras1hasbeenshowntoexhibitclock-dependentrhythminmicesuprachiasmaticnucleusSCNanditsgeneticdeletionmodulatescircadianresponsestophoticandnonphoticcues.WeshowthattherhythmicexpressionofDexras1mRNAandproteininratSCNalreadyoscillateswithlowamplitudeatpostnatalday3andcanbedetectedasearlyasembryonicday20.Incontrastitsexpressioninperipheraltissuesisnotrhythmicinadultratseither.TheDexras1proteinisexpressedpredominantlyinthedorsomedialpartoftheSCNandthelightpulsehasonlyalimitedeffectonitsexpression.OurdataprovidethedescriptivebasisforspeculationabouttheDexras1involvementintheratcircadianphysiology.date2016sectionpartNumberpartTitleDOI10.310907420528.2015.1120741citationKeyurlPMIDPMCIDISSN0742-0528languagecollectionsGE86BF5KdateModified2025-03-19T115834ZkeyC7MD5G9Ylibraryid5891878metacreatorSummaryFliegeretal.parsedDate2016numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtFliegerM.BandouchovaH.CernyJ.ChudickovaM.KolarikM.KovacovaV.MartinkovaN.NovakP.SebestaO.StodulkovaE.PikulaJ.ltbgtVitaminB-2asaVirulenceFactorinPseudogymnoascusDestructansSkinInfectionltbgt.ltigtSCIENTIFICREPORTSltigtltbgt2016ltbgtltigt6ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.1038srep33200039gthttpsdoi.org10.1038srep33200ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleVitaminB-2asavirulencefactorinPseudogymnoascusdestructansskininfectioncreatorscreatorTypeauthorfirstNameMiroslavlastNameFliegercreatorTypeauthorfirstNameHanalastNameBandouchovacreatorTypeauthorfirstNameJanlastNameCernycreatorTypeauthorfirstNameMiladalastNameChudickovacreatorTypeauthorfirstNameMiroslavlastNameKolarikcreatorTypeauthorfirstNameVeronikalastNameKovacovacreatorTypeauthorfirstNameNatalialastNameMartinkovacreatorTypeauthorfirstNamePetrlastNameNovakcreatorTypeauthorfirstNameOndrejlastNameSebestacreatorTypeauthorfirstNameEvalastNameStodulkovacreatorTypeauthorfirstNameJirilastNamePikulaabstractNotePathogenicandnon-pathogenicrelatedmicroorganismsdifferinsecondarymetaboliteproduction.Hereweshowthatriboflavinoverproductionbyafungalpathogenanditshyperaccumulationinaffectedhosttissueexacerbatesaskininfectiontonecrosis.Inwhite-nosesyndromeWNSskinlesionscausedbyPseudogymnoascusdestructansmaximumriboflavinconcentrationsreachedupto815mugml1indicatingbioaccumulationandlackofexcretion.Wefoundthathighriboflavinconcentrationsarecytotoxicunderconditionsspecificforhibernationaffectbats039primaryfibroblastsandinducecelldetachmentlossofmitochondrialmembranepotentialpolymerizationofcorticalactinandcellnecrosis.OurresultsexplainmolecularpathologyofWNSwhereaskininfectionbecomesfatal.HyperaccumulationofvitaminB-2coupledwithreducedmetabolismandlowtissueoxygensaturationduringhibernationpreventsremovalofexcessriboflavinininfectedbats.Uponreperfusionoxygenreactswithriboflavinresultingindramaticpathologyafterarousal.Whilemultiplemoleculesenableinvasiveinfectionriboflavin-associatedextensivenecrosislikelycontributestopathophysiologyandalteredarousalpatternininfectedbats.Bioaccumulationofavitaminundernaturalinfectionrepresentsanovelconditioninacomplexhost-pathogeninterplay.date2016sectionpartNumberpartTitleDOI10.1038srep33200citationKeyurlPMIDPMCIDISSN2045-2322languagecollectionsGE86BF5KdateModified2025-03-19T115834ZkeyVGINYCJPlibraryid5891878metacreatorSummaryMelkesetal.parsedDate2016numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMelkesB.HejnovaL.NovotnyJ.ltbgtBiasedMu-OpioidReceptorAgonistsDiverselyRegulateLateralMobilityandFunctionalCouplingoftheReceptortoItsCognateGProteinsltbgt.ltigtNAUNYN-SCHMIEDEBERGSARCHIVESOFPHARMACOLOGYltigtltbgt2016ltbgtltigt389ltigt121289x20131300.ltaclass039zp-DOIURL039href039httpsdoi.org10.1007s00210-016-1293-8039gthttpsdoi.org10.1007s00210-016-1293-8ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleBiasedmu-opioidreceptoragonistsdiverselyregulatelateralmobilityandfunctionalcouplingofthereceptortoitscognateGproteinscreatorscreatorTypeauthorfirstNameBarboralastNameMelkescreatorTypeauthorfirstNameLucielastNameHejnovacreatorTypeauthorfirstNameJirilastNameNovotnyabstractNoteTherearesomeindicationsthatbiasedmu-opioidligandsmaydiverselyaffectmu-opioidreceptorMORproperties.HereweusedconfocalfluorescencerecoveryafterphotobleachingFRAPtostudytheregulationbydifferentMORagonistsofreceptormovementwithintheplasmamembraneofHEK293cellsstablyexpressingafunctionalyellowfluorescentproteinYFP-taggedmu-opioidreceptorMOR-YFP.WefoundthatthelateralmobilityofMOR-YFPwasincreasedbyD-Ala2N-MePhe4Gly5-ol-enkephalinDAMGOandtoalesserextentalsobymorphinebutdecreasedbyendomorphin-2.InterestinglycholesteroldepletionstronglyenhancedtheabilityofmorphinetoelevatereceptormobilitybutsignificantlyreducedoreveneliminatedtheeffectofDAMGOandendomorphin-2respectively.MoreovertheabilityofDAMGOandendomorphin-2toinfluenceMOR-YFPmovementwasdiminishedbypertussistoxintreatment.Theresultsobtainedbyagonist-stimulatedS-35GTPgammaSbindingassaysindicatedthatDAMGOexhibitedhigherefficacythanmorphineandendomorphin-2didandthattheefficacyofDAMGOcontrarytothelatteragonistswasenhancedbycholesteroldepletion.OverallourstudyprovidesclearevidencethatbiasedMORagonistsdiverselyaffectreceptormobilityinplasmamembranesaswellasMORGproteincouplingandthattheregulatoryeffectofdifferentligandsdependsonthemembranecholesterolcontent.ThesefindingshelptodelineatethefundamentalpropertiesofMORregardingtheirinteractionwithbiasedMORligandsandcognateGproteins.date2016sectionpartNumberpartTitleDOI10.1007s00210-016-1293-8citationKeyurlPMIDPMCIDISSN0028-1298languagecollectionsGE86BF5KdateModified2025-03-19T115834Zkey74IE7DVXlibraryid5891878metacreatorSummaryJurjevicetal.parsedDate2015-12numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtJurjevicZ.KubatovaA.KolarikM.HubkaV.ltbgtTaxonomyofAspergillusSectionPetersoniiSect.NovEncompassingIndoorandSoil-BorneSpecieswithPredominantTropicalDistributionltbgt.ltigtPlantSyst.Evol.ltigtltbgt2015ltbgtltigt301ltigt102441x20132462.ltaclass039zp-DOIURL039href039httpsdoi.org10.1007s00606-015-1248-4039gthttpsdoi.org10.1007s00606-015-1248-4ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTaxonomyofAspergillussectionPetersoniisect.novencompassingindoorandsoil-bornespecieswithpredominanttropicaldistributioncreatorscreatorTypeauthorfirstNameZeljkolastNameJurjeviccreatorTypeauthorfirstNameAlenalastNameKubatovacreatorTypeauthorfirstNameMiroslavlastNameKolarikcreatorTypeauthorfirstNameVitlastNameHubkaabstractNoteDuringmouldsurveysanumberofAspergillusstrainswereisolatedfromenvironmentalairwhichdidnotfitanyknownspeciesofthegenus.TheyshowedphenotypicaswellasmoleculargeneticsimilaritywithA.arenariusA.arenarioidesandA.peyroneliithreespecieswithoutclearphylogeneticposition.Multi-genephylogeneticanalysiscomprisingtaxaacrossthesubgenusCircumdatishowedthatthesespeciesclusterintoawell-supportedcladesistertosect.Candidi.Weproposethestatusofanewsectionforthiscladesect.Petersoniisect.nov.Thephenotypicdescriptionsafter14dayson8variousagarmediawereprovidedformembersofsect.PetersoniiwhichtogetherwithmaximumgrowthtemperatureandmoleculargeneticdatafromfourlociITSrDNAbeta-tubulincalmodulinandRPB2supportedtherecognitionoffourspecies.TwospeciesarenewlydescribedhereasA.asclerogenussp.nov.andA.petersoniisp.nov.AspergillusarenariusisreducedtosynonymywithA.peyroneliiaspeciesrevivedandtypifiedinthisstudy.Adichotomouskeybasedonthecombinationofmorphologyandphysiologyisprovidedforallrecognizedspeciesofsect.Petersonii.Inadditionotherspeciesfromsubg.CircumdatiwithambiguousphylogeneticpositionbasedonpreviousstudieswerealsoincludedinouranalysisresultingintheproposalofsectionsRobustiandTanneri.Allnewlyproposedsectionsalsohavestrongphenotypicsupport.dateDEC2015sectionpartNumberpartTitleDOI10.1007s00606-015-1248-4citationKeyurlPMIDPMCIDISSN0378-26971615-6110languageEnglishcollectionsGE86BF5KdateModified2025-11-07T093417ZkeyZJTL8DAWlibraryid5891878metacreatorSummaryHubkaetal.parsedDate2015-11numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHubkaV.NissenC.V.JensenR.H.ArendrupM.C.CmokovaA.KubatovaA.SkorepovaM.KolarikM.ltbgtDiscoveryofaSexualStageinTrichophytonOnychocolaaPresumedGeophilicDermatophyteIsolatedfromToenailsofPatientswithaHistoryofT.RubrumOnychomycosisltbgt.ltigtMed.Mycol.ltigtltbgt2015ltbgtltigt53ltigt8798x2013809.ltaclass039zp-DOIURL039href039httpsdoi.org10.1093mmymyv044039gthttpsdoi.org10.1093mmymyv044ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDiscoveryofasexualstageinTrichophytononychocolaapresumedgeophilicdermatophyteisolatedfromtoenailsofpatientswithahistoryofT.rubrumonychomycosiscreatorscreatorTypeauthorfirstNameVitlastNameHubkacreatorTypeauthorfirstNameChristofferV.lastNameNissencreatorTypeauthorfirstNameRasmusHarelastNameJensencreatorTypeauthorfirstNameMaikenC.lastNameArendrupcreatorTypeauthorfirstNameAdelalastNameCmokovacreatorTypeauthorfirstNameAlenalastNameKubatovacreatorTypeauthorfirstNameMagdalenalastNameSkorepovacreatorTypeauthorfirstNameMiroslavlastNameKolarikabstractNoteTrichophytononychocolaisarecentlydescribedgeophilicdermatophytethathasbeenisolatedfromatoenailofCzechpatientwithahistoryofonychomycosisduetoT.rubrumandclinicalsuspicionofrelapse.InthisstudywereportasimilarcasefromDenmarkinanotherwisehealthy56-year-oldman.ThepatienthadahistoryofgreattoenailinfectioncausedbyT.rubrumin2004andpresentedwithsuspectedrelapsein2011and2013.Trichophytononychocolawastheonlymicrobialagentisolatedatthesecondvisitin2013andtheidentificationwasconfirmedbyDNAsequencing.DirectmicroscopicnailexaminationwaspositiveforhyphaehowevertheetiologicalsignificanceofT.onychocolawasnotsupportedbyrepeatedisolationofthefungus.ThisnewspeciesmaybeanoverlookedgeophilicspeciesduetotheresemblancetosomecommonspeciesforexamplezoophilicT.interdigitaleorsomespeciesofgeophilicdermatophytes.Weincludeddifferentialdiagnosiswithphenotypicallysimilarspecieshoweveritisrecommendedthatmolecularmethodsareusedforcorrectidentification.TheMATlocusofDanishstrainwasofoppositematingtypethaninthepreviouslyisolatedCzechstrainandthetwoisolatesweresuccessfullymated.ThematingexperimentswithrelatedheterothallicspeciesT.thuringienseandArthrodermameliswerenegative.Thesexualstateshowedalltypicalsignsofarthroderma-morphandisdescribedbyusingopticalaswellasscanningelectronmicroscopy.Thesexualstatewasinducedonasetofagarmediahoweverlowcultivationtemperatureandthepresenceofkeratinsourcewerecrucialforthesuccessratherthanformulationofmedium.dateNOV2015sectionpartNumberpartTitleDOI10.1093mmymyv044citationKeyurlPMIDPMCIDISSN1369-37861460-2709languageEnglishcollectionsGE86BF5KdateModified2025-11-07T094032Zkey7DYBVYDDlibraryid5891878metacreatorSummaryNemcovaetal.parsedDate2015-08-14numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtNemcovaY.PichrtovaM.ZeisekV.ltbgtMallomonasAlpestrinaSp.Nov.SynuralesChrysophyceaeStramenopilesandItsSpinelessRelativesx2014MallomonasAlataGroupltbgt.ltigtPhytotaxaltigtltbgt2015ltbgtltigt222ltigt2111.ltaclass039zp-DOIURL039href039httpsdoi.org10.11646phytotaxa.222.2.3039gthttpsdoi.org10.11646phytotaxa.222.2.3ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMallomonasalpestrinasp.nov.SynuralesChrysophyceaeStramenopilesanditsspinelessrelativesu2014MallomonasalatagroupcreatorscreatorTypeauthorfirstNameYvonnelastNameNemcovacreatorTypeauthorfirstNameMartinalastNamePichrtovacreatorTypeauthorfirstNameVojtechlastNameZeisekabstractNoteInthispaperwedefinetheMallomonasalatagroupanddescribeM.alpestrinasp.nov.fromanoligotrophichighmountainglaciallakeontheslopesofHabaXueShanHabaSnowMountainChina.TheMallomonasalatagroupisexcludedfromtheM.pumiliogroupprimarilybasedontheapproximatelytriangularshapeofthecollarscalesthesmallhook-likeprotrudeddomeandoneconsiderablybroaderanteriorflangeofthebodyscale.WeextendpreviousresearchonsmallspeciesfromthesectionTorquataewithreticulatedscale-shieldpattern.MoleculargeneticdatafortheMallomonasalatagroupspeciesarenotcurrentlyavailablethereforeweprovidedetailedinformationonscaleandscale-casemorphologyenvironmentalrequirementsandgeographicaldistributionofthesespecies.date2015-08-14sectionpartNumberpartTitleDOI10.11646phytotaxa.222.2.3citationKeyurlhttpsbiotaxa.orgPhytotaxaarticleviewphytotaxa.222.2.3PMIDPMCIDISSN1179-31631179-3155languagecollectionsGE86BF5KdateModified2025-11-07T094545ZkeyKSJX4FR7libraryid5891878metacreatorSummaryHernychovaetal.parsedDate2015-07numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHernychovaL.MrazekH.GrobarovaV.KukackaZ.SebestaO.CernyJ.NovakP.ltbgtStructuralandFunctionalCharacterizationoftheMouseInhibitoryC-TypeLectin-likeReceptorltbgt.ltigtFEBSJ.ltigtltbgt2015ltbgtltigt282ltigt341x2013342.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleStructuralandfunctionalcharacterizationofthemouseinhibitoryC-typelectin-likereceptorcreatorscreatorTypeauthorfirstNameL.lastNameHernychovacreatorTypeauthorfirstNameH.lastNameMrazekcreatorTypeauthorfirstNameV.lastNameGrobarovacreatorTypeauthorfirstNameZ.lastNameKukackacreatorTypeauthorfirstNameO.lastNameSebestacreatorTypeauthorfirstNameJ.lastNameCernycreatorTypeauthorfirstNameP.lastNameNovakabstractNotedateJUL2015sectionpartNumberpartTitleDOIcitationKeyurlPMIDPMCIDISSN1742-464X1742-4658languageEnglishcollectionsGE86BF5KdateModified2025-03-19T115834ZkeyMI27H2RNlibraryid5891878metacreatorSummaryDolejsetal.parsedDate2014-09numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtDolejsP.BucharJ.KubcovaL.SmrzJ.ltbgtDevelopmentalChangesintheSpinningApparatusovertheLifeCycleofWolfSpidersAraneaeLycosidaeltbgt.ltigtInvertebr.Biol.ltigtltbgt2014ltbgtltigt133ltigt3281x2013297.ltaclass039zp-DOIURL039href039httpsdoi.org10.1111ivb.12055039gthttpsdoi.org10.1111ivb.12055ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDevelopmentalchangesinthespinningapparatusoverthelifecycleofwolfspidersAraneaeLycosidaecreatorscreatorTypeauthorfirstNamePetrlastNameDolejscreatorTypeauthorfirstNameJanlastNameBucharcreatorTypeauthorfirstNameLenkalastNameKubcovacreatorTypeauthorfirstNameJaroslavlastNameSmrzabstractNoteSpidersarecharacterizedbytheirspinningactivity.Muchofthecurrentknowledgeofthespinningapparatuscomesfromstudiesonorbwebspidersandtheirrelativeswhereaswolfspidershavebeenmoreorlessneglectedinthisrespect.ThereforewestudieddevelopmentalchangesinthespinningapparatusoffourwolfspidersTriccalutetianaArctosaalpigenalampertiPardosaamentataandXerolycosanemoralisthroughouttheirlifecycles.Eachoftheselycosidshasastenochronouslifecyclebutofvariedlengthfrom1to3yearsandnumberofinstarsfromseventoten.Useofthespinningapparatusbeginsinthefirstinstarafterleavingtheeggsac.Secondaryampullateallpiriformandallbutfouraciniformglandsaretartipore-accommodated.Thetartiporescollaredopeningsthroughwhichsilkglandductspassduringproecdysisappearonthespinningfieldstartingwiththesecondinstar.Tartipore-accommodatedglandscanfunctionduringproecdysisandtheirevolutioncorrespondswiththewayspiderssecurethemselveswhenmolting.Wesuggestthatthefunctionofaciniformsilkinjuvenilewolfspidersistoserveasanancillaryquotscaffoldquotsupportingthespider039sbodyduringecdysis.dateSEP2014sectionpartNumberpartTitleDOI10.1111ivb.12055citationKeyurlhttpswww.webofscience.comapigatewayGWVersion2SrcAuthGetFTRSrcAppWOSDestURLhttps3A2F2Fct.prod.getft.io2FY2xhcml2YXRlLHdpbGV5LGh0dHBzOi8vb25saW5lbGlicmFyeS53aWxleS5jb20vZG9pL3BkZi8xMC4xMTExL2l2Yi4xMjA1NQ.nMMUZUPccwegYdAbhhDOnsXvlqKP1V-k3ukIZB115ZMDestAppGetFTRSrcItemIdWOS000341179600008SrcAppSIDEUW1ED0AD5l35ynCpYiKbmzlvxhzSHMACo3rbkT0kBGGLOhBVUI1btNWbbqY3RcaJsusOX2FHe4YE3DPMIDPMCIDISSN1077-83061744-7410languageEnglishcollectionsGE86BF5KdateModified2025-11-07T094029ZkeyQ5RM6NENlibraryid5891878metacreatorSummaryZilaetal.parsedDate2014-05-08numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtZilaV.DifatoF.KlimovaL.HuerfanoS.ForstovaJ.ltbgtInvolvementofMicrotubularNetworkandItsMotorsinProductiveEndocyticTraffickingofMousePolyomavirusltbgt.ltigtPLOSONEltigtltbgt2014ltbgtltigt9ltigt5e96922.ltaclass039zp-DOIURL039href039httpsdoi.org10.1371journal.pone.0096922039gthttpsdoi.org10.1371journal.pone.0096922ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleInvolvementofMicrotubularNetworkandItsMotorsinProductiveEndocyticTraffickingofMousePolyomaviruscreatorscreatorTypeauthorfirstNameVojtechlastNameZilacreatorTypeauthorfirstNameFrancescolastNameDifatocreatorTypeauthorfirstNameLucielastNameKlimovacreatorTypeauthorfirstNameSandralastNameHuerfanocreatorTypeauthorfirstNameJitkalastNameForstovaabstractNoteInfectionofnon-envelopedpolyomavirusesdependsonanintactmicrotubularnetwork.HerewefocusonmousepolyomavirusMPyV.WeshowthatthedynamicsofMPyVcytoplasmictransportreflectsthecharacteristicsofmicrotubularmotor-driventransportwithbi-directionalsaltatorymovements.Incellstreatedwithmicrotubule-disruptingagentslocalizationofMPyVwassignificantlyperturbedtheviruswasretainedatthecellperipherymostlywithinmembranestructuresresemblingmulticaveolarcomplexesandatlatertimespost-infectiononlyafractionoftheviruswasfoundinRab7-positiveendosomesandmultivesicularbodies.Inhibitionofcytoplasmicdynein-basedmotilitybyoverexpressionofdynamitinaffectedperinucleartranslocationofthevirusdeliveryofvirionstotheERandsubstantiallyreducedthenumbersofinfectedcellswhileoverexpressionofdominant-negativeformofkinesin-1orkinesin-2hadnosignificantimpactonviruslocalizationandinfectivity.WealsofoundthattransportalongmicrotubuleswasimportantforMPyV-containingendosomesequentialacquisitionofRab5Rab7andRab11GTPases.Howeverincontrasttodominant-negativemutantofRab7T22Noverexpressionofdominant-negativemutantRab11S25Ndidnotaffectthevirusinfectivity.AltogetherourstudyrevealedthatMPyVcytoplasmictraffickingleadingtoproductiveinfectionbypassesrecyclingendosomesdoesnotrequirethefunctionofkinesin-1andkinesin-2butdependsonfunctionaldynein-mediatedtransportalongmicrotubulesfortranslocationofthevirionsfromperipheraloftencaveolin-positivecompartmentstolateendosomesandERu2013aprerequisiteforefficientdeliveryoftheviralgenometothenucleus.dateMay82014sectionpartNumberpartTitleDOI10.1371journal.pone.0096922citationKeyurlhttpsjournals.plos.orgplosonearticleid10.1371journal.pone.0096922PMIDPMCIDISSN1932-6203languageencollectionsGE86BF5KdateModified2025-03-07T102151ZkeyN36XEM6Tlibraryid5891878metacreatorSummaryBartosiketal.parsedDate2014numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtBartosikA.A.GlabskiK.JeczP.LasockiK.MikosaM.PlochockaD.ThomasC.M.Jagura-BurdzyG.ltbgtDissectionoftheRegionofltigtPseudomonasAeruginosaltigtParAThatIsImportantforDimerizationandInteractionswithItsPartnerParBltbgt.ltigtMICROBIOLOGY-SGMltigtltbgt2014ltbgtltigt160ltigt2406x20132420.ltaclass039zp-DOIURL039href039httpsdoi.org10.1099mic.0.081216-0039gthttpsdoi.org10.1099mic.0.081216-0ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDissectionoftheregionofiPseudomonasaeruginosaiParAthatisimportantfordimerizationandinteractionswithitspartnerParBcreatorscreatorTypeauthorfirstNameA.A.lastNameBartosikcreatorTypeauthorfirstNameK.lastNameGlabskicreatorTypeauthorfirstNameP.lastNameJeczcreatorTypeauthorfirstNameK.lastNameLasockicreatorTypeauthorfirstNameM.lastNameMikosacreatorTypeauthorfirstNameD.lastNamePlochockacreatorTypeauthorfirstNameC.M.lastNameThomascreatorTypeauthorfirstNameG.lastNameJagura-BurdzyabstractNotePseudomonasaeruginosaParAbelongstoalargesubfamilyofWalker-typeATPasesactingaspartitioningproteinsinbacteria.ParAhastheabilitytobothself-associateandinteractwithitspartnerParB.AnalysisofthedeletionmutantsdefinedthepartoftheproteininvolvedindimerizationandinteractionswithParB.HereasetofParAalaninesubstitutionmutantsintheregionbetweenE67andL85wascreatedandanalysedinvivoandinvitro.AllmutantsimpairedindimerizationsubstitutionsatpositionsM74H79Y82andL84werealsodefectiveininteractionswithParBsuggestingthatParAParBinteractionsdependontheabilityofParAtodimerize.MutantswithalaninesubstitutionsatpositionsE67C68L70E72F76083andL85werenotimpairedindimerizationbutweredefectiveininteractionswithParB.Thedimerizationinterfacepartlyoverlappedthepseudo-hairpininvolvedininteractionswithParB.ParAmutantderivativestestedinvitroshowednodefectsinATPaseactivity.TwoparAallelesparA84whoseproductcanneitherself-interactnorinteractwithParBandparA67whoseproductisimpairedininteractionswithParBbutnotindimerizationwereintroducedintotheP.aeruginosachromosomebyhomologousgeneexchange.BothmutantsshoweddefectiveseparationofParBfocibuttodifferentextents.OnlyPAO1161parA84wasvisiblyimpairedintermsofchromosomesegregationgrowthrateandmotilitysimilartoaparA-nullmutant.date2014sectionpartNumberpartTitleDOI10.1099mic.0.081216-0citationKeyurlPMIDPMCIDISSN1350-0872languagecollectionsGE86BF5KdateModified2025-03-19T115836Zkey4TBKI424libraryid5891878metacreatorSummaryPurkartovaetal.parsedDate2014numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtPurkartovaZ.TumaJ.PestaM.KuldaV.HajkovaL.SebestaO.VozehF.CendelinJ.ltbgtMorphologicalAnalysisofEmbryonicCerebellarGraftsinSCA2Miceltbgt.ltigtNEUROSCIENCELETTERSltigtltbgt2014ltbgtltigt558ltigt154x2013158.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.neulet.2013.11.020039gthttpsdoi.org10.1016j.neulet.2013.11.020ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMorphologicalanalysisofembryoniccerebellargraftsinSCA2micecreatorscreatorTypeauthorfirstNameZdenkalastNamePurkartovacreatorTypeauthorfirstNameJanlastNameTumacreatorTypeauthorfirstNameMartinlastNamePestacreatorTypeauthorfirstNameVlastimillastNameKuldacreatorTypeauthorfirstNameLucielastNameHajkovacreatorTypeauthorfirstNameOndrejlastNameSebestacreatorTypeauthorfirstNameFrantiseklastNameVozehcreatorTypeauthorfirstNameJanlastNameCendelinabstractNoteSCA2transgenicmicearethoughttobeausefulmodelofhumanspinocerebellarataxiatype2.Thereisnoeffectivetherapyforcerebellardegenerativedisordersthereforeneurotransplantationcouldofferhope.TheaimofthisworkwastoassessthesurvivalandmorphologyofembryoniccerebellargraftstransplantedintothecerebellumofadultSCA2mice.Fourmonth-oldhomozygousSCA2andnegativecontrolmiceweretreatedwithbilateralintracerebellarinjectionsofanenhancedgreenfluorescentprotein-positiveembryoniccerebellarcellsuspension.Graftsurvivalandmorphologywereexaminedthreemonthslater.Graft-derivedPurkinjecellsandthepresenceofastrocytesinthegraftweredetectedimmunohistochemically.Nisslandhematoxylineosintechniqueswereusedtovisualizethehistologicalstructureofthegraftandsurroundinghosttissue.GraftssurvivedinallexperimentalmicenodifferencesingraftstructurebetweenSCA2homozygousandnegativemicewerefound.ThegraftscontainednumerousPurkinjecellsbutlongdistancegraft-to-hostaxonalconnectionstothedeepcerebellarnucleiwererarelyseen.Relativelyfewastrocyteswerefoundinthecenterofthegraft.Nosignsofinflammationortissuedestructionwereseenintheareaaroundthegrafts.Despitegoodgraftsurvivalandthepresenceofgraft-derivedPurkinjecellsthestructureofthegraftdidnotseemtopromiseanysignificantspecificfunctionaleffects.Wehaveshownthatthegraftisavailableforlong-termexperiments.Neverthelessitwouldbebeneficialtosearchforwaysofenhancementofconnectionsbetweenthegraftandhost.C2013ElsevierIrelandLtd.Allrightsreserved.date2014sectionpartNumberpartTitleDOI10.1016j.neulet.2013.11.020citationKeyurlPMIDPMCIDISSN0304-3940languagecollectionsGE86BF5KdateModified2025-03-19T115834ZkeyT2UT33K8libraryid5891878metacreatorSummaryHylisetal.parsedDate2013-09numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHylisM.ObornikM.NebesarovaJ.VavraJ.ltbgtDescriptionandPhylogenyofZelenkaiaTrichopteraeGen.etSpNovMicrosporidiaanAquaticMicrosporidianParasiteofCaddisfliesTrichopteraFormingSporeDoubletsltbgt.ltigtJ.Invertebr.Pathol.ltigtltbgt2013ltbgtltigt114ltigt111x201321.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.jip.2013.04.010039gthttpsdoi.org10.1016j.jip.2013.04.010ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDescriptionandphylogenyofZelenkaiatrichopteraegen.etspnovMicrosporidiaanaquaticmicrosporidianparasiteofcaddisfliesTrichopteraformingsporedoubletscreatorscreatorTypeauthorfirstNameMiroslavlastNameHyliscreatorTypeauthorfirstNameMiroslavlastNameObornikcreatorTypeauthorfirstNameJanalastNameNebesarovacreatorTypeauthorfirstNameJirilastNameVavraabstractNoteTwonovelmicrosporidiainfectingthefatbodytissuesinlarvaeoftwohostsHalesusdigitatusandMicropternasequaxTrichopteraLimnephilidaewereinvestigatedusinglightandelectronmicroscopyandrDNAsequenceanalyses.ThemolecularandmorphologicalcharactersoftheseisolateswarrantcreationofanewmicrosporidiangenusZelenkaiagen.n.withtwospeciesonenamedherein.DevelopmentalstagesofZelenkaiaspp.havesinglenuclei.Insporogonyaplasmodiumwithfournucleigivesrisebyrosette-likebuddingtotwopairsofuninucleatesporoblastseachwithinathin-walledsubpersistentsporophorousvesicle.Sporoblastsandmaturesporesadheretemporarytogetherformingdoubletsorientedinparallelwithinthesporophorousvesicle.Sporesarelong-ovalanduninucleateandthoseofthetypespecies1trichopteraemeasure10.3x3.5mumandhave24-25polarfilamentcoils.PhylogeneticanalysisbasedonrDNAplacesZelenkaiaspp.withintheaquaticcladeofmicrosporidiaandmorespecificallyinthecladecontainingsomemicroporidiafromamphipodhosts.C2013ElsevierInc.Allrightsreserved.dateSEP2013sectionpartNumberpartTitleDOI10.1016j.jip.2013.04.010citationKeyurlPMIDPMCIDISSN0022-20111096-0805languageEnglishcollectionsGE86BF5KdateModified2025-11-07T094027ZkeyCLRYLYISlibraryid5891878metacreatorSummaryHubkaetal.parsedDate2013-08numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHubkaV.DobiasovaS.LyskovaP.MallatovaN.ChlebkovaJ.SkorepovaM.KubatovaA.DobiasR.ChudickovaM.KolarikM.ltbgtAuxarthronOstravienseSpNov.andA.UmbrinumAssociatedwithNon-DermatophyticOnychomycosisltbgt.ltigtMed.Mycol.ltigtltbgt2013ltbgtltigt51ltigt6614x2013624.ltaclass039zp-DOIURL039href039httpsdoi.org10.310913693786.2013.770608039gthttpsdoi.org10.310913693786.2013.770608ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleAuxarthronostraviensespnov.andA.umbrinumassociatedwithnon-dermatophyticonychomycosiscreatorscreatorTypeauthorfirstNameVitlastNameHubkacreatorTypeauthorfirstNameStanislavalastNameDobiasovacreatorTypeauthorfirstNamePavlinalastNameLyskovacreatorTypeauthorfirstNameNadalastNameMallatovacreatorTypeauthorfirstNameJanalastNameChlebkovacreatorTypeauthorfirstNameMagdalenalastNameSkorepovacreatorTypeauthorfirstNameAlenalastNameKubatovacreatorTypeauthorfirstNameRadimlastNameDobiascreatorTypeauthorfirstNameMiladalastNameChudickovacreatorTypeauthorfirstNameMiroslavlastNameKolarikabstractNoteAuxarthronisagenuswithintheOnygenalesencompassingkeratinophilicspecieswithtypicalascomatagymnotheciaconsistingofanastomosingnetworkofthick-walledhyphaeandsmallgloboseoroblateascospores.Noassociationofthisgenuswithclinicallyrelevantcasesofhumanoranimalinfectionhasbeenreported.ThispaperdescribestheisolationofanundescribedAuxarthronspeciesasanagentofprovenonychomycosisaffectingalmostallfingernailsinamanwithpsoriasis.Thecausalityoftheisolatedfunguswasverifiedbyrepeatedsamplinganddirectmicroscopyrevealingirregularseptatehyphae.Basedonmicro-andmacromorphologicalfeaturesanduniquesequencedataITSregionbenAandRPB2genetheisolatedfungusisproposedasthenewspeciesA.ostraviense.ThesiblingspeciesofA.ostravienseA.umbrinumwasisolatedfromthreepatientswithsuspectedonychomycosisandadetailedclinicalhistoryisprovidedforoneofthesepatients.Allfourisolatesweretestedforsusceptibilitytoselectedantifungalagents.Terbinafineandclotrimazoleappeartobeeffectiveinvitro.ThemorphologicalidentificationofAuxarthronspp.isnon-trivialtime-consumingandrequirescultivationmediaotherthanSabouraudglucoseagarwhichisroutinelyusedindermatomycology.dateAUG2013sectionpartNumberpartTitleDOI10.310913693786.2013.770608citationKeyurlPMIDPMCIDISSN1369-37861460-2709languageEnglishcollectionsGE86BF5KdateModified2025-11-07T094022ZkeyRWCPJCYQlibraryid5891878metacreatorSummaryKutalovaetal.parsedDate2013-07numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKutalovaK.BourguignonT.Sillam-DussesD.HanusR.RoisinY.SobotnikJ.ltbgtArmedReproductivesEvolutionoftheFrontalGlandinImagoesofTermitidaeltbgt.ltigtArthropodStruct.Dev.ltigtltbgt2013ltbgtltigt42ltigt4339x2013348.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.asd.2013.04.001039gthttpsdoi.org10.1016j.asd.2013.04.001ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleArmedreproductivesEvolutionofthefrontalglandinimagoesofTermitidaecreatorscreatorTypeauthorfirstNameKatetinalastNameKutalovacreatorTypeauthorfirstNameThomaslastNameBourguignoncreatorTypeauthorfirstNameDavidlastNameSillam-DussescreatorTypeauthorfirstNameRobertlastNameHanuscreatorTypeauthorfirstNameYveslastNameRoisincreatorTypeauthorfirstNameJanlastNameSobotnikabstractNoteThefrontalglandoftermitesisastructurewithoutanyequivalentamongotheranimals.Althoughthisglandiswellknowninsoldiersitreceivedalmostnoattentioninothercastes.RecentlywedescribeditinimagoesofRhinotermitidaeandSerritermitidae.Inordertoprovideacompletepictureoftheevolutionofthisglandintermiteimagoeswestudieditinadditional34speciesofTermitidaerepresenting7ofthe8subfamilies.Thefrontalglandofthesespeciesisformedbyclass1secretorycellsonlyandoccursintwobasicshapesepithelialwithreservoirinForaminitermitinaeandMacrotermitinaeandepithelialwithoutreservoirinallothersubfamilies.ThesizevariabilityoftheglandishighnotonlyamongTermitidaesubfamiliesbutalsowithinsubfamilies.OurdatasuggestthattheancestralformofthefrontalglandisepithelialwithreservoirasfoundinRhinotermitidaeSerritermitidaeandbasalTermitidae.ThereductionofthereservoiroccurredatleasttwotimesandtheglandwaslosttwotimesindependentlyinProtermessp.andinMicrotermestoumodiensisbothMacrotermitinae.C2013ElsevierLtd.Allrightsreserved.dateJUL2013sectionpartNumberpartTitleDOI10.1016j.asd.2013.04.001citationKeyurlPMIDPMCIDISSN1467-80391873-5495languageEnglishcollectionsGE86BF5KdateModified2025-11-07T094017ZkeyL3CHRFJTlibraryid5891878metacreatorSummaryFormanetal.parsedDate2013-05-22numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtFormanM.NguyenP.HulaV.KrxE1lJ.ltbgtSexChromosomePairingandExtensiveNORPolymorphisminWadicosaFidelisAraneaeLycosidaeltbgt.ltigtCytogeneticandGenomeResearchltigtltbgt2013ltbgtltigt141ltigt143x201349.ltaclass039zp-ItemURL039href039httpsdoi.org10.1159000351041039gthttpsdoi.org10.1159000351041ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleSexChromosomePairingandExtensiveNORPolymorphisminWadicosafidelisAraneaeLycosidaecreatorscreatorTypeauthorfirstNameM.lastNameFormancreatorTypeauthorfirstNameP.lastNameNguyencreatorTypeauthorfirstNameV.lastNameHulacreatorTypeauthorfirstNameJ.lastNameKru00e1labstractNoteIntermsofcytogeneticsentelegynearaneomorphsarethebeststudiedcladeofspiders.Thetypicalkaryotypeofentelegynemalesconsistsofacrocentricchromosomesincluding2non-homologousXchromosomes.ThepresentstudyisfocusedonthekaryotypenucleolusorganisingregionsNORsandsexchromosomebehaviourduringmeiosisoftheentelegyneWadicosafidelisLycosidae.PreparationsstainedbyGiemsawereusedtostudykaryotypeandmeiosis.NORswerevisualisedbysilverstainingandfluorescenceinsituhybridisationwith18SrDNAprobe.Themalekaryotypeconsistsof28acrocentricelementsincluding2Xchromosomes.Incontrasttothemajorityofotherspidersthemalesexchromosomespairduringthemajorpartofmeiosis.FollowinganinitialperiodofparallelpairingtheattachmentofmalesexchromosomesisrestrictedtocentromericareasandcontinuesuntilmetaphaseII.OurstudyrevealedanenormousnumberofNORsinthepopulationfromGalileeandindicatesaconsiderablevariabilityofNORnumbersinthispopulation.Thedistalregionsof9or10autosomalpairscontainNORs.TheobtaineddataindicatetherapidspreadofNORsinthekaryotypeofW.fideliswhichwaspresumablycausedbyectopicrecombinationsandsubsequenthybridisationsofindividualswithdifferentNORgenotypesthatproducedheterozygotes.date2013-05-22sectionpartNumberpartTitleDOI10.1159000351041citationKeyurlhttpsdoi.org10.1159000351041PMIDPMCIDISSN1424-8581languagecollectionsGE86BF5KdateModified2025-03-07T100455Zkey439AF75Rlibraryid5891878metacreatorSummaryHubkaetal.parsedDate2013-02numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHubkaV.PetersonS.W.FrisvadJ.C.YaguchiT.KubatovaA.KolarikM.ltbgtAspergillusWaksmaniiSpNovandAspergillusMarvanovaeSpNov.TwoCloselyRelatedSpeciesinSectionFumigatiltbgt.ltigtInt.J.Syst.Evol.Microbiol.ltigtltbgt2013ltbgtltigt63ltigt783x2013789.ltaclass039zp-DOIURL039href039httpsdoi.org10.1099ijs.0.047076-0039gthttpsdoi.org10.1099ijs.0.047076-0ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleAspergilluswaksmaniispnovandAspergillusmarvanovaespnov.twocloselyrelatedspeciesinsectionFumigaticreatorscreatorTypeauthorfirstNameVitlastNameHubkacreatorTypeauthorfirstNameStephenW.lastNamePetersoncreatorTypeauthorfirstNameJensC.lastNameFrisvadcreatorTypeauthorfirstNameTakashilastNameYaguchicreatorTypeauthorfirstNameAlenalastNameKubatovacreatorTypeauthorfirstNameMiroslavlastNameKolarikabstractNoteTwonewandphylogeneticallycloselyrelatedspeciesinAspergillussectionFumigatiaredescribedandillustrated.HomothallicAspergilluswaksmaniisp.nov.wasisolatedfromNewJerseysoilUSAandisrepresentedbytheex-typeisolateNRRL179TCCF4266TThom4138.HS2TIBT31900T.Aspergillusmarvanovaesp.nov.wasisolatedfromwaterwithhighboracicacidanionscontentinDukovanynuclearpowerstationCzechRepublic.ThesexualstageofthisspeciesisunknownbuttheMAT1-1locuswassuccessfullyamplifiedsuggestingthatthespeciesisprobablyheterothallicandteleomorphicbutisrepresentedbyonlytheex-typeisolateCCM8003TCCF4037TNRRL62486TIBT31279TIFM60873T.BothspeciescanbedistinguishedfromallpreviouslydescribedspeciesinsectionFumigatibasedonmorphologymaximumgrowthtemperaturesequencedatafromfiveunlinkedlocianduniquesecondarymetabolitesprofiles.dateFEB2013sectionpartNumberpartTitleDOI10.1099ijs.0.047076-0citationKeyurlPMIDPMCIDISSN1466-50261466-5034languageEnglishcollectionsGE86BF5KdateModified2025-11-07T094019ZkeyZHT6RYARlibraryid5891878metacreatorSummaryVu00e1vraetal.parsedDate2013-01-01numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtVxE1vraJ.Hylix161M.ObornxEDkM.VossbrinckC.R.ltbgtMicrosporidiainAquaticMicrocrustaceaTheCopepodMicrosporidiumMarssoniellaElegansLemmermann1900Revisitedltbgt.ltigtFoliaParasitologicaltigtltbgt2013ltbgtltigt52ltigt1x20132163x2013172.ltaclass039zp-ItemURL039href039httpsdoi.org10.14411fp.2005.021039gthttpsdoi.org10.14411fp.2005.021ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMicrosporidiainaquaticmicrocrustaceathecopepodmicrosporidiumMarssoniellaelegansLemmermann1900revisitedcreatorscreatorTypeauthorfirstNameJiu0159u00edlastNameVu00e1vracreatorTypeauthorfirstNameMiroslavlastNameHyliu0161creatorTypeauthorfirstNameMiroslavlastNameObornu00edkcreatorTypeauthorfirstNameCharlesR.lastNameVossbrinckabstractNoteJiu0159u00edVu00e1vraMiroslavHyliu0161MiroslavObornu00edkCharlesR.Vossbrinckdate201311sectionpartNumberpartTitleDOI10.14411fp.2005.021citationKeyurlhttpsdoi.org10.14411fp.2005.021PMIDPMCIDISSN0015568318036465languageencollectionsGE86BF5KdateModified2025-11-07T094629ZkeySP5RMVEZlibraryid5891878metacreatorSummaryHuerfanoetal.parsedDate2013numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHuerfanoS.RyabchenkoB.ForstovxE1J.ltbgtNucleofectionofExpressionVectorsInducesaRobustInterferonResponseandInhibitionofCellProliferationltbgt.ltigtDNAandCellBiologyltigtltbgt2013ltbgtltigt32ltigt8467x2013479.ltaclass039zp-DOIURL039href039httpsdoi.org10.1089dna.2012.1950039gthttpsdoi.org10.1089dna.2012.1950ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleNucleofectionofExpressionVectorsInducesaRobustInterferonResponseandInhibitionofCellProliferationcreatorscreatorTypeauthorfirstNameSandralastNameHuerfanocreatorTypeauthorfirstNameBorislastNameRyabchenkocreatorTypeauthorfirstNameJitkalastNameForstovu00e1abstractNotedate082013sectionpartNumberpartTitleDOI10.1089dna.2012.1950citationKeyurlhttpwww.liebertpub.comdoi10.1089dna.2012.1950PMIDPMCIDISSN1044-54981557-7430languageencollectionsGE86BF5KdateModified2025-03-07T114039ZkeyXRDCC9B9libraryid5891878metacreatorSummaryWaskova-Arnostovaetal.parsedDate2013numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtWaskova-ArnostovaP.ElsnicovaB.KasparovaD.SebestaO.NovotnyJ.NeckarJ.KolarF.ZurmanovaJ.ltbgtRight-To-LeftVentricularDifferencesintheExpressionofMitochondrialHexokinaseandPhosphorylationofAktltbgt.ltigtCELLULARPHYSIOLOGYANDBIOCHEMISTRYltigtltbgt2013ltbgtltigt31ltigt166x201379.ltaclass039zp-DOIURL039href039httpsdoi.org10.1159000343350039gthttpsdoi.org10.1159000343350ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleRight-To-LeftVentricularDifferencesintheExpressionofMitochondrialHexokinaseandPhosphorylationofAktcreatorscreatorTypeauthorfirstNamePetralastNameWaskova-ArnostovacreatorTypeauthorfirstNameBarbaralastNameElsnicovacreatorTypeauthorfirstNameDitalastNameKasparovacreatorTypeauthorfirstNameOndrejlastNameSebestacreatorTypeauthorfirstNameJirilastNameNovotnycreatorTypeauthorfirstNameJanlastNameNeckarcreatorTypeauthorfirstNameFrantiseklastNameKolarcreatorTypeauthorfirstNameJitkalastNameZurmanovaabstractNoteBackgroundAimsHexokinaseHKisakeyglycolyticenzymewhichpromotesthemaintenanceofglucosehomeostasisincardiomyocytes.HK1isoformispredominantlyboundtotheoutermitochondrialmembraneandhighlysupportsoxidativephosphorylationbyincreasingtheavailabilityofADPforcomplexVoftherespiratorychain.HK2isoformisunderphysiologicalconditionspredominantlylocalizedinthecytosolanduponstimulationofPI3KAktpathwayassociateswithmitochondriaandthuscanpreventapoptosis.ThepurposeofthisstudywastoinvestigateexpressionandsubcellularlocalizationofbothHKisoformsinleftLVandrightRVheartventriclesofadultmaleWistarrats.MethodsReal-TimeRT-PCRWesternblottingandquantitativeimmunofluorescencemicroscopywereused.ResultsOurresultsshowedasignificantlyhigherexpressionofbothHK1andHK2atmRNAandproteinlevelsintheRVcomparedtotheLV.ThesefindingswerecorroboratedbyimmunofluorescencestainingwhichrevealedsubstantiallyhigherfluorescencesignalsofbothHKsintheRVthanintheLV.Theratiosofphospho-Ser473-Aktnon-phospho-Aktandphospho-Thr308-Aktnon-phospho-AktwerealsomarkedlyhigherintheRVthanintheLV.ConclusionTheseresultssuggestthattheRVhasahigheractivityofaerobicglycolyticmetabolismandmaybeabletorespondfasterandmorepowerfullytostressfulstimulithantheLV.CopyrightC2013S.KargerAGBaseldate2013sectionpartNumberpartTitleDOI10.1159000343350citationKeyurlPMIDPMCIDISSN1015-8987languagecollectionsGE86BF5KdateModified2025-03-19T115834ZkeyYPNRZHILlibraryid5891878metacreatorSummaryJindrovu00e1etal.parsedDate2012-11numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtJindrovxE1A.Tx16FmaJ.SlxE1dekV.ltbgtIntra-ObserverErrorofMouseLongBoneCrossSectionDigitizationltbgt.ltigtfozoltigtltbgt2012ltbgtltigt61ltigt3x20134340x2013349.ltaclass039zp-DOIURL039href039httpsdoi.org10.25225fozo.v61.i3.a1.2012039gthttpsdoi.org10.25225fozo.v61.i3.a1.2012ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleIntra-observererrorofmouselongbonecrosssectiondigitizationcreatorscreatorTypeauthorfirstNameAlenalastNameJindrovu00e1creatorTypeauthorfirstNameJanlastNameTu016fmacreatorTypeauthorfirstNameVladimu00edrlastNameSlu00e1dekabstractNoteDigitizationofperiostealandendostealcontoursofbonecrosssectionisoneofseveralmethodsusedforcalculatinglongbonecross-sectionalgeometryCSG.Inthisstudyinvasivelyobtainedbonehistologicalsampleswereusedtocalculateintra-observermeasurementerrorforCSGparameters.Intra-observererrorwasmeasuredbaseduponrepeatedmeasurementsofcross-sectionalareastotalareaTAcorticalareaCAandmomentsofareaImaxIminImaxIminJinB6CBAmicen17.Crosssectionswerecutat50ofthebiomechanicallengthofthelefttibiaandthesampleswerefurtherprocessedforCSGandhistologicalanalysis.Intra-observererrorwasmeasuredtoestimatetheaccuracyofthedigitizationmethod.AccuracyofthetesteddigitizationmethodwasexpressedbymeandifferenceMDmeanabsolutedifferenceMADandlimitsofagreementLA.Theresultsconfirmourassumptionthatintra-observererrordecreaseswiththenumberofrepeatedmeasurementevents.Thustheerrorcanbeminimizedbyacquiringexperienceinthesectiondigitizing.OurresultsalsoshowthatTACAandpolarmomentofareaJaremoresusceptibletointra-observererrorthanareImaxIminandImaxImin.date201211sectionpartNumberpartTitleDOI10.25225fozo.v61.i3.a1.2012citationKeyurlhttpsbioone.orgjournalsfolia-zoologicavolume-61issue-3e280934fozo.v61.i3.a1.2012Intra-observer-error-of-mouse-long-bone-cross-section-digitization10.25225fozo.v61.i3.a1.2012.fullPMIDPMCIDISSN0139-78931573-1189languagecollectionsGE86BF5KdateModified2025-03-07T103044ZkeyWMXE9BU2libraryid5891878metacreatorSummaryVilu00edmovu00e1andKutalovu00e1parsedDate2012numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtVilxEDmovxE1J.KutalovxE1K.ltbgtOccurrenceofCertainCuticularStructuresConfirmsFunctionalityofDorsalAbdominalScentGlandsinAcanthosomatidaeHeteropteraPentatomoidealtbgt.ltigtBull.Entomol.Res.ltigtltbgt2012ltbgtltigt102ltigt129x201342.ltaclass039zp-DOIURL039href039httpsdoi.org10.1017S0007485311000344039gthttpsdoi.org10.1017S0007485311000344ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleOccurrenceofcertaincuticularstructuresconfirmsfunctionalityofdorsalabdominalscentglandsinAcanthosomatidaeHeteropteraPentatomoideacreatorscreatorTypeauthorfirstNameJ.lastNameVilu00edmovu00e1creatorTypeauthorfirstNameK.lastNameKutalovu00e1abstractNoteAbstractnnElasmuchaferrugatanFabricius1787HeteropteraAcanthosomatidaeprovidesmaternalcareofeggsandlarvae.AdultsofbothsexeshavefunctionalanteriordorsalabdominalscentglandsDAGs.StudyofallinternalandexternalcuticularstructuresofDAGsrevealedthatnoDAGsarefullyfunctionalinthe1stlarvalinstarandveryprobablytheyareonlyslightlyfunctionalinthe2ndinstar.MedianandposteriorDAGsareundoubtedlynotfunctionalinadults.Thereexistssexualdimorphisminthenumberofmulticellularglandularunitsinanteriorglandsinadults.Theoccurrenceofcuticularductulesoftheseunitsprovesthesetobefunctionalglands.Thisisbestconsideredincombinationwiththefindingsofawell-developedevaporatorium.Developedcuticularintimaoftheglandreservoirandorthenearlyclosedostioleorostiolarscarbearsnoinformationaboutthefunctionalityofthegland.date022012sectionpartNumberpartTitleDOI10.1017S0007485311000344citationKeyurlhttpswww.cambridge.orgcoreproductidentifierS0007485311000344typejournal_articlePMIDPMCIDISSN0007-48531475-2670languageencollectionsGE86BF5KdateModified2025-11-07T094829ZkeyUNQYG2WMlibraryid5891878metacreatorSummaryPau017eoutovu00e1etal.parsedDate2012numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtPax17EoutovxE1S.x160rx16FtkaP.Holux161aJ.ChudxEDx10DkovxE1M.KubxE1tovxE1A.Kolax159xEDkM.ltbgtltigtLiberomycesltigtGen.Nov.withTwoNewSpeciesofEndophyticCoelomycetesfromBroadleafTreesltbgt.ltigtMycologialtigtltbgt2012ltbgtltigt104ltigt1198x2013210.ltaclass039zp-DOIURL039href039httpsdoi.org10.385211-081039gthttpsdoi.org10.385211-081ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleiLiberomycesigen.nov.withtwonewspeciesofendophyticcoelomycetesfrombroadleaftreescreatorscreatorTypeauthorfirstNameSylvielastNamePau017eoutovu00e1creatorTypeauthorfirstNamePetrlastNameu0160ru016ftkacreatorTypeauthorfirstNameJaroslavlastNameHoluu0161acreatorTypeauthorfirstNameMiladalastNameChudu00edu010dkovu00e1creatorTypeauthorfirstNameAlenalastNameKubu00e1tovu00e1creatorTypeauthorfirstNameMiroslavlastNameKolau0159u00edkabstractNotedate012012sectionpartNumberpartTitleDOI10.385211-081citationKeyurlhttpswww.tandfonline.comdoifull10.385211-081PMIDPMCIDISSN0027-55141557-2536languageencollectionsGE86BF5KdateModified2025-11-07T094251ZkeyPHT6JRL5libraryid5891878metacreatorSummaryDejmkovaetal.parsedDate2012numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtDejmkovaH.ZimaJ.BarekJ.MikaJ.ltbgtBehaviorofGlassyCarbonPasteElectrodeinFlowingMethanolicSolutionsltbgt.ltigtELECTROANALYSISltigtltbgt2012ltbgtltigt24ltigt81766x20131770.ltaclass039zp-DOIURL039href039httpsdoi.org10.1002elan.201100598039gthttpsdoi.org10.1002elan.201100598ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleBehaviorofGlassyCarbonPasteElectrodeinFlowingMethanolicSolutionscreatorscreatorTypeauthorfirstNameH.lastNameDejmkovacreatorTypeauthorfirstNameJ.lastNameZimacreatorTypeauthorfirstNameJ.lastNameBarekcreatorTypeauthorfirstNameJ.lastNameMikaabstractNoteCarbonpasteelectrodesareoftenusedforelectrochemicaldeterminationsbuttheirapplicationinmediawithhighcontentoforganicsolventsparticularlyinHPLCislimitedbytheirlowstability.Theworkpresentedhereexaminestheeffectofamovingmethanol-containingsolutiononaglassycarbonpasteelectrodebystudyingitselectrochemicalbehaviorinrelationtotheperiodofcontactandmethanolcontentinthesolution.Incombinationwithmicroscopicobservationofthepastesurfaceitcanbeconcludedthattheelectrodeisaffectedbythemethanolicsolutionandthesurfaceroughnessincreasesresultinginthestablestatecompatiblewithmeasurementsinhighlymethanolicsolutions.date2012sectionpartNumberpartTitleDOI10.1002elan.201100598citationKeyurlPMIDPMCIDISSN1040-0397languagecollectionsGE86BF5KdateModified2025-03-19T115836Zkey98VRC4WAlibraryid5891878metacreatorSummaryHornu00edkovu00e1etal.parsedDate2011-12-01numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHornxEDkovxE1L.ManP.ForstovxE1J.ltbgtBlueNativeProteinElectrophoresisforStudiesofMousePolyomavirusMorphogenesisandInteractionsbetweentheMajorCapsidProteinVP1andCellularProteinsltbgt.ltigtJournalofVirologicalMethodsltigtltbgt2011ltbgtltigt178ltigt1229x2013234.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.jviromet.2011.08.019039gthttpsdoi.org10.1016j.jviromet.2011.08.019ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleBluenativeproteinelectrophoresisforstudiesofmousepolyomavirusmorphogenesisandinteractionsbetweenthemajorcapsidproteinVP1andcellularproteinscreatorscreatorTypeauthorfirstNameLenkalastNameHornu00edkovu00e1creatorTypeauthorfirstNamePetrlastNameMancreatorTypeauthorfirstNameJitkalastNameForstovu00e1abstractNoteMorphogenesisofthemousepolyomavirusvirionisacomplexandnotyetwellunderstoodprocess.NuclearlysatesofinfectedcellsandcellstransientlyproducingthemajorcapsidproteinVP1ofthemousepolyomavirusandwhole-celllysateswereseparatedbybluenativepolyacrylamidegelelectrophoresisBN-PAGEtocharacterizetheparticipationofcellularproteinsinvirionprecursorcomplexes.SeveralVP1-specificcomplexeswerefoundbyimmunostainingwiththeanti-VP1antibody.Someofthesecomplexescontainedproteinsfromtheheatshockprotein70family.TheBN-PAGEwasfoundtobeausefultoolfortheidentificationofproteincomplexesbyimmunostainingofseparatedcelllysates.Howeverwhole-celllysatesandlysatesofisolatednucleiofcellsinfectedwithpolyomavirusappearedtobetoocomplexforBN-PAGEseparationfollowedbymassspectrometry.NodistinctbandsspecificforcellsinfectedwithpolyomavirusweredetectedbyCoomassiebluestainedgelshencethismethodisnotsuitableforthediscoveryofnewcellularproteinsparticipatinginvirionassembly.NeverthelessBN-PAGEcanbevaluablefortheanalysesofdifferenttypesofcomplexesformedbyproteinsaftertheirenrichmentorisolationbyaffinitychromatography.date2011-12-01sectionpartNumberpartTitleDOI10.1016j.jviromet.2011.08.019citationKeyurlhttpswww.sciencedirect.comsciencearticlepiiS0166093411003533PMIDPMCIDISSN0166-0934languagecollectionsGE86BF5KdateModified2025-03-07T102549Zkey4AUWE3A2libraryid5891878metacreatorSummaryBulantovu00e1etal.parsedDate2011-01-01numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtBulantovxE1J.ChanovxE1M.Houx17Evix10DkovxE1L.HorxE1kP.ltbgtltigtTrichobilharziaRegentiltigtDigeneaSchistosomatidaeChangesofBodyWallMusculatureduringtheDevelopmentfromMiracidiumtoAdultWormltbgt.ltigtMicronltigtltbgt2011ltbgtltigt42ltigt147x201354.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.micron.2010.08.003039gthttpsdoi.org10.1016j.micron.2010.08.003ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleiTrichobilharziaregentiiDigeneaSchistosomatidaeChangesofbodywallmusculatureduringthedevelopmentfrommiracidiumtoadultwormcreatorscreatorTypeauthorfirstNameJanalastNameBulantovu00e1creatorTypeauthorfirstNameMartalastNameChanovu00e1creatorTypeauthorfirstNameLenkalastNameHouu017eviu010dkovu00e1creatorTypeauthorfirstNamePetrlastNameHoru00e1kabstractNoteTrichobilharziaregentiSchistosomatidaeDigeneaaparasiteofbirdsexhibitsauniquestrategyamongschistosomeshavingaffinitytothenervoussystemofvertebratehosts.Migrationofparasiticstageswithinhostsandorswimmingofnon-parasiticlarvaeinwaterenvironmentdependontheactionofbodywallmuscleswhichwerestudiedwithconfocalandelectronmicroscopy.Inallstagesbodywallmusculatureiscomprisedofdifferentlyorganizedcircularandlongitudinalmuscles.Duringthedevelopmentanextensivechangeofmusculaturecharacteristicsandorformationofnewmusclestructureswererecordedcercariaeschistosomulaandadultwormsproduceadditionalunderlyingdiagonalmusclefibersandinnerplexusofradialmusculature.Substantialchangesoftheouterenvironmentduringpenetrationofahostosmoticvaluesofwatervs.hosttissuesareaccompaniedbysurfacetransformationofmiracidiamothersporocystsandcercariaeschistosomula.Contrarytothatchangesofbodymusculatureinthesestagesarecharacterizedonlybygrowthandre-organizationofexistingstructuresandneverbyformationofnewcomponentsofbodymusculature.Futurestudiesinthisfieldmaycontributetoabetterknowledgeofmorphologyandfunctionoftrematodemusclesincludingthoseofschistosomesthatareimportantpathogensofhumansandanimals.date2011-01-01sectionpartNumberpartTitleDOI10.1016j.micron.2010.08.003citationKeyurlhttpswww.sciencedirect.comsciencearticlepiiS0968432810001848PMIDPMCIDISSN0968-4328languagecollectionsGE86BF5KdateModified2025-03-10T080441ZkeyJMSINDVWlibraryid5891878metacreatorSummaryConradetal.parsedDate2011-01-01numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtConradM.ZubacovaZ.DunnL.A.UpcroftJ.SullivanS.A.TachezyJ.CarltonJ.M.ltbgtMicrosatellitePolymorphismintheSexuallyTransmittedHumanPathogenltigtTrichomonasVaginalisltigtIndicatesaGeneticallyDiverseParasiteltbgt.ltigtMolecularandBiochemicalParasitologyltigtltbgt2011ltbgtltigt175ltigt130x201338.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.molbiopara.2010.08.006039gthttpsdoi.org10.1016j.molbiopara.2010.08.006ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMicrosatellitepolymorphisminthesexuallytransmittedhumanpathogeniTrichomonasvaginalisiindicatesageneticallydiverseparasitecreatorscreatorTypeauthorfirstNameMelissalastNameConradcreatorTypeauthorfirstNameZuzanalastNameZubacovacreatorTypeauthorfirstNameLindaA.lastNameDunncreatorTypeauthorfirstNameJacquilastNameUpcroftcreatorTypeauthorfirstNameStevenA.lastNameSullivancreatorTypeauthorfirstNameJanlastNameTachezycreatorTypeauthorfirstNameJaneM.lastNameCarltonabstractNoteGiventhegrowingappreciationofserioushealthsequelaefromwidespreadTrichomonasvaginalisinfectionnewtoolsareneededtostudytheparasite039sgeneticdiversity.Tothisendwehaveidentifiedandcharacterizedapanelof21microsatellitesandsixsingle-copygenesfromtheT.vaginalisgenomeusingsevenlaboratorystrainsofdiverseorigin.Wehave1adaptedourmicrosatellitetypingmethodtoincorporateaffordablefluorescentlabeling2determinedthatthemicrosatellitelociremainstableinparasitescontinuouslyculturedforupto17monthsand3evaluatedmicrosatellitemarkercoverageofthesixchromosomesthatcomprisetheT.vaginalisgenomeusingfluorescentinsituhybridizationFISH.WehaveusedthemarkerstoshowthatT.vaginalisisageneticallydiverseparasiteinapopulationofcommonlyusedlaboratorystrains.Inadditionwehaveusedphylogeneticmethodstoinferevolutionaryrelationshipsfromourmarkersinordertovalidatetheirutilityinfuturepopulationanalyses.OurpanelisthefirstseriesofrobustpolymorphicgeneticmarkersforT.vaginalisthatcanbeusedtoclassifyandmonitorlabstrainsaswellasprovideameanstomeasurethegeneticdiversityandpopulationstructureofextantandfutureT.vaginalisisolates.date2011-01-01sectionpartNumberpartTitleDOI10.1016j.molbiopara.2010.08.006citationKeyurlhttpswww.sciencedirect.comsciencearticlepiiS016668511000229XPMIDPMCIDISSN0166-6851languagecollectionsGE86BF5KdateModified2025-03-07T094416ZkeyFMPZLFLJlibraryid5891878metacreatorSummaryKepkovaetal.parsedDate2011numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtKepkovaK.V.VodickaP.ToralovaT.LopatarovaM.CechS.DolezelR.HavlicekV.BesenfelderU.KuzmanyA.SirardM.A.LaurincikJ.KankaJ.ltbgtTranscriptomicAnalysisofltigtinVivoltigtandltigtinVitroltigtProducedBovineEmbryosRevealedaDevelopmentalChangeinCullin1ExpressionduringMaternal-to-EmbryonicTransitionltbgt.ltigtTHERIOGENOLOGYltigtltbgt2011ltbgtltigt75ltigt91582x20131595.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.theriogenology.2010.12.019039gthttpsdoi.org10.1016j.theriogenology.2010.12.019ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTranscriptomicanalysisofiinvivoiandiinvitroiproducedbovineembryosrevealedadevelopmentalchangeincullin1expressionduringmaternal-to-embryonictransitioncreatorscreatorTypeauthorfirstNameK.V.lastNameKepkovacreatorTypeauthorfirstNameP.lastNameVodickacreatorTypeauthorfirstNameT.lastNameToralovacreatorTypeauthorfirstNameM.lastNameLopatarovacreatorTypeauthorfirstNameS.lastNameCechcreatorTypeauthorfirstNameR.lastNameDolezelcreatorTypeauthorfirstNameV.lastNameHavlicekcreatorTypeauthorfirstNameU.lastNameBesenfeldercreatorTypeauthorfirstNameA.lastNameKuzmanycreatorTypeauthorfirstNameM.A.lastNameSirardcreatorTypeauthorfirstNameJ.lastNameLaurincikcreatorTypeauthorfirstNameJ.lastNameKankaabstractNotePre-implantationembryosderivedbyinvitrofertilizationdifferintheirdevelopmentalpotentialfromembryosobtainedinvivo.Inordertocharacterizechangesingeneexpressionprofilescausedbyinvitrocultureenvironmentweemployedmicroarrayconstructedfrombovineoocyteandpreimplantationembryo-specificcDNAsBlueChipUniversiteLavalQuebec.Theanalysisrevealedchangesinthelevelof134transcriptsbetweeninvitroderivedculturedinCOOKBVCBVBmediaandinvivoderived4-cellstageembryosand97transcriptsweredifferentiallyexpressedbetween8-cellstageinvitroandinvivoembryos.Theexpressionprofilesof7selectedtranscriptsBUB3CUL1FBLNOLC1PCAFGABPAandCNOT4werestudiedindetail.WehaveidentifiedaswitchfromCullin1-liketranscriptvariant1toCullin1transcriptvariant3UniGeneIDsBT.36789andBT.6490respectivelyexpressionsaroundthetimeofbovinemajorgeneactivation8-cellstage.Newfibrillarinproteinwasdetectedbyimmunofluorescencealreadyinearly8-cellstageandthisdetectioncorrelatedwithincreasedleveloffibrillarinmRNA.TheqRT-PCRanalysisrevealedsignificantdifferencesinthelevelofBUB3NOLC1PCAFGABPAandCNOT4genetranscriptsbetweeninvivoderivedIVDandinvitroproducedIVPembryosinlate8-cellstage.ThecombinationofthesegenesrepresentsasuitabletoolforaddressingquestionsconcerningnormalIVDembryodevelopmentandcanbepotentiallyusefulasamarkerofembryoqualityinfutureattemptstooptimizeinvitrocultureconditions.C2011ElsevierInc.Allrightsreserved.date2011sectionpartNumberpartTitleDOI10.1016j.theriogenology.2010.12.019citationKeyurlPMIDPMCIDISSN0093-691XlanguagecollectionsGE86BF5KdateModified2025-03-19T115836Zkey5Y79WZF6libraryid5891878metacreatorSummaryLigasovaetal.parsedDate2011numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtLigasovaA.BulantovaJ.SebestaO.KasnyM.KobernaK.MikesL.ltbgtSecretoryGlandsinCercariaoftheNeuropathogenicSchistosomeTrichobilharziaRegenti-UltrastructuralCharacterization3-DModellingVolumeandpHEstimationsltbgt.ltigtPARASITESampVECTORSltigtltbgt2011ltbgtltigt4ltigt.ltaclass039zp-DOIURL039href039httpsdoi.org10.11861756-3305-4-162039gthttpsdoi.org10.11861756-3305-4-162ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleSecretoryglandsincercariaoftheneuropathogenicschistosomeTrichobilharziaregenti-ultrastructuralcharacterization3-DmodellingvolumeandpHestimationscreatorscreatorTypeauthorfirstNameAnnalastNameLigasovacreatorTypeauthorfirstNameJanalastNameBulantovacreatorTypeauthorfirstNameOndrejlastNameSebestacreatorTypeauthorfirstNameMartinlastNameKasnycreatorTypeauthorfirstNameKarellastNameKobernacreatorTypeauthorfirstNameLiborlastNameMikesabstractNoteBackgroundCercariaeofschistosomesemploybioactivemoleculesforpenetrationintotheirhosts.Thesearereleasedfromspecializedunicellularglandsuponstimulifromhostskin.Theglandswerepreviouslywell-describedinthehumanpathogenSchistosomamansoni.AsbirdschistosomescanalsopenetratehumanskinandcausecercarialdermatitisouraimwastocharacterizethearchitectureandultrastructureofglandsintheneurotropicbirdschistosomeTrichobilharziaregentiandcompareitwithS.mansoni.InthecontextofdifferenthistolyticenzymesusedbythesetwospecieswefocusedalsoontheestimationsofglandvolumesandpHinT.regenti.ResultsThearchitectureand3-Dmodelsoftwotypesofacetabularpenetrationglandstheirductsandoftheheadglandareshownhere.WecharacterizedsecretoryvesiclesinallthreeglandtypesbymeansofTEMandconfirmedaccuracyofthemodelsobtainedbyconfocalmicroscopy.Theresultsoftwoindependentapproachesshowedthattheglandsoccupyca.onethirdofcercarialbodyvolumepostacetabularglandsca.15circumacetabular12andheadgland6.TheinnerenvironmentwithinthetwotypesofacetabularglandsdifferedsignificantlyasevidencedbydissimilarabilitytobindfluorescentmarkersandbypHvaluewhichwashigherincircumacetabular7.44thaninpostacetabular7.08glands.ConclusionsAsfarasweknowthisisthefirstpresentationofa3-Dmodelofcercarialglandsandthefirstexactestimationofthevolumesofthethreeglandtypesinschistosomes.OurcomparisonsbetweenT.regentiandS.mansoniimpliedthatthearchitectureandultrastructureoftheglandsismostlikelyconservedwithinthefamily.Onlyminorvariationswerefoundbetweenthetwospecies.ItseemsthatthedifferencesinmolecularcompositionhavenoeffectongeneralappearanceofthesecretorycellsinTEM.Fluorescentmarkersemployedinthisstudydistinguishingbetweensecretoryvesiclesandglandtypescanbeusefulinfurtherstudiesofmechanismsusedbycercariaeforhostinvasion.ResultsofthefirstattemptstoestimatepHwithinschistosomeglandsmayhelpfurtherunderstandingofregulationofenzymaticactivitiespresentwithintheglands.date2011sectionpartNumberpartTitleDOI10.11861756-3305-4-162citationKeyurlPMIDPMCIDISSN1756-3305languagecollectionsGE86BF5KdateModified2025-03-19T115834ZkeySIAEVBIMlibraryid5891878metacreatorSummaryDoubravskaetal.parsedDate2011numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtDoubravskaL.KrausovaM.GradlD.VojtechovaM.TumovaL.LukasJ.ValentaT.PospichalovaV.FafilekB.PlachyJ.SebestaO.KorinekV.ltbgtFattyAcidModificationofWnt1andWnt3aatSerineIsPrerequisiteforLipidationatCysteineandIsEssentialforWntSignallingltbgt.ltigtCELLULARSIGNALLINGltigtltbgt2011ltbgtltigt23ltigt5837x2013848.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.cellsig.2011.01.007039gthttpsdoi.org10.1016j.cellsig.2011.01.007ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleFattyacidmodificationofWnt1andWnt3aatserineisprerequisiteforlipidationatcysteineandisessentialforWntsignallingcreatorscreatorTypeauthorfirstNameLenkalastNameDoubravskacreatorTypeauthorfirstNameMichaelalastNameKrausovacreatorTypeauthorfirstNameDietmarlastNameGradlcreatorTypeauthorfirstNameMartinalastNameVojtechovacreatorTypeauthorfirstNameLucielastNameTumovacreatorTypeauthorfirstNameJanlastNameLukascreatorTypeauthorfirstNameTomaslastNameValentacreatorTypeauthorfirstNameVendulalastNamePospichalovacreatorTypeauthorfirstNameBohumillastNameFafilekcreatorTypeauthorfirstNameJirilastNamePlachycreatorTypeauthorfirstNameOndrejlastNameSebestacreatorTypeauthorfirstNameVladimirlastNameKorinekabstractNoteTheWntfamilyofproteinsisagroupofextracellularsignallingmoleculesthatregulatecell-fatedecisionsindevelopingandadulttissues.Itispresumedthatall19mammalianWntfamilymemberscontaintwotypesofpost-translationalmodificationthecovalentattachmentoffattyacidsattwodistinctpositionsandtheN-glycosylationofmultipleasparagines.WeexaminedhowthesemodificationscontributetothesecretionextracellularmovementandsignallingactivityofmouseWnt1andWnt3aligands.WerevealedthatO-linkedacylationofserineisrequiredforthesubsequentS-palmitoylationofcysteine.Assuchmutantproteinsthatlackthecrucialserineresiduearenotlipidated.Interestinglyalthoughdouble-acylationofWnt1wasindispensableforsignallinginmammaliancellsinXenopusembryostheS-palmitoyl-deficientformretainedthesignallingactivity.InthecaseofWnt3athefunctionaldualityoftheattachedacylswaslessprominentsincetheligandlackingS-linkedpalmitatewasstillcapableofsignallinginvariouscellularcontexts.FinallyweshowthatthesignallingcompetencyofbothWnt1andWnt3aisrelatedtotheirabilitytoassociatewiththeextracellularmatrix.C2011ElsevierInc.Allrightsreserved.date2011sectionpartNumberpartTitleDOI10.1016j.cellsig.2011.01.007citationKeyurlPMIDPMCIDISSN0898-6568languagecollectionsGE86BF5KdateModified2025-03-19T115834ZkeyJ87IB4L2libraryid5891878metacreatorSummaryJurackaetal.parsedDate2010-12-09numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtJurackaP.J.KorinekV.PetrusekA.ltbgtANewCentralEuropeanSpeciesoftheDaphniaCurvirostrisComplexDaphniaHrbacekiSpNovCladoceraAnomopodaDaphniidaeltbgt.ltigtZootaxaltigtltbgt2010ltbgtNo.27181x201322.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleAnewCentralEuropeanspeciesoftheDaphniacurvirostriscomplexDaphniahrbacekispnovCladoceraAnomopodaDaphniidaecreatorscreatorTypeauthorfirstNamePetrJanlastNameJurackacreatorTypeauthorfirstNameVladimirlastNameKorinekcreatorTypeauthorfirstNameAdamlastNamePetrusekabstractNoteAlthoughsystematicsofthecladocerangenusDaphniaCladoceraDaphniidaehasbeenintensivelyinvestigatedfordecadesusingbothmorphologicalandgeneticapproachesnewlineagesarebeingdiscoveredonallcontinentsincludinginwell-studiedregions.AmongHolarcticdaphnidsDaphniacurvirostrisEylmann1887heldaninterestingpositionsharingsomemorphologicalcharactersofboththeD.pulexandD.longispinagroups.RecentlyadditionalspeciesoftheD.curvirostriscomplexhavebeendiscoveredintheEasternPalaearctic.HerewedescribeanewspeciesinthiscomplexfromCentralEuropeD.hrbacekisp.nov.ItwasdiscoveredinsmallnewlycreatedfishlesspoolsintheCzechRepublicandanadditionalsampleofapparentlythesametaxonwascollectedin1951inSlovakia.D.hrbacekiistheclosestyetknownrelativeofD.curvirostrisbutremainsgeneticallydivergentfromallmembersofthecomplexbasedonthesequencesofthreemitochondrialgenes12SCOIandND2.IngeneraladultfemalesofthisspeciesaremorphologicallyverysimilartoD.curvirostris.UnlikethelatterspeciesD.hrbacekimaydevelopaspecifichump-shapeddorsaloutlineofthecarapacepresumablyaninducibledefenceagainstinvertebratepredators.Juvenilesofthenewspeciesoccasionallyformneckteethwhichmayalsoberetainedinadultindividuals.ThespeciesalsoshowssubstantialvariationinthesizeofspinesinthemiddlepectenofthepostabdominalclawsimilarlyasintheJapanesememberofthespeciescomplexD.tanakaiIshidaKotovampTaylor2006.Thisvariablecharacterofspinesizeinthepostabdominalmiddlepectenatransitionfromthepulextothelongispinagroupcharacteraswellasabentandheavilysetulatedterminalsetaonthemale2ndendopoditeconsideredasthepulexgroupcharacteraretypicalforthenewspecies.D.hrbacekialsodiffersfromD.curvirostrisaswellasothermembersofthecomplexintheephippialsurfaceultrastructure.OurstudydemonstratestheutilityofsuchultrastructuralcharactersinDaphniataxonomicalstudies.dateDEC92010sectionpartNumberpartTitleDOIcitationKeyurlPMIDPMCIDISSN1175-53261175-5334languageEnglishcollectionsGE86BF5KdateModified2025-11-07T094008ZkeyJTBU7D4Qlibraryid5891878metacreatorSummaryVrkoslavetal.parsedDate2010-02-01numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtVrkoslavV.MuckA.Cvax10DkaJ.Svatox161A.ltbgtMALDIImagingofNeutralCuticularLipidsinInsectsandPlantsltbgt.ltigtJ.Am.Soc.MassSpectrom.ltigtltbgt2010ltbgtltigt21ltigt2220x2013231.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.jasms.2009.10.003039gthttpsdoi.org10.1016j.jasms.2009.10.003ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMALDIimagingofneutralcuticularlipidsininsectsandplantscreatorscreatorTypeauthorfirstNameVladimu00edrlastNameVrkoslavcreatorTypeauthorfirstNameAlexanderlastNameMuckcreatorTypeauthorfirstNameJoseflastNameCvau010dkacreatorTypeauthorfirstNameAleu0161lastNameSvatou0161abstractNotedate2010-02-01sectionpartNumberpartTitleDOI10.1016j.jasms.2009.10.003citationKeyurlhttpspubs.acs.orgdoi10.1016j.jasms.2009.10.003PMIDPMCIDISSN1044-0305languageencollectionsGE86BF5KdateModified2025-11-07T094313ZkeyFFQKTFE7libraryid5891878metacreatorSummaryDolejsetal.parsedDate2010numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtDolejsP.KubcovaL.BucharJ.ltbgtCourtshipMatingandCocoonMaintenanceofTriccaLutetianaAraneaeLycosidaeltbgt.ltigtJ.Arachnol.ltigtltbgt2010ltbgtltigt38ltigt3504x2013510.ltaclass039zp-DOIURL039href039httpsdoi.org10.1636Hi09-29.1039gthttpsdoi.org10.1636Hi09-29.1ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleCourtshipmatingandcocoonmaintenanceofTriccalutetianaAraneaeLycosidaecreatorscreatorTypeauthorfirstNamePetrlastNameDolejscreatorTypeauthorfirstNameLenkalastNameKubcovacreatorTypeauthorfirstNameJanlastNameBucharabstractNoteTriccahaetianaSimon1876Lycosidaeliveshiddenundergroundandthusisnotwellknown.Ourobjectivewastodocumentmorefullybasicinformationonreproductionparticularlycopulationinthisspecies.Weobtainedandobservedinthelaboratory86individualsfromthewildbetween2006and2008.Vibratoryandtactilecommunicationisanimportantmediumduringsexualcommunication.Wedescribeduniquemovementsofthematingmale039slegsduringcopulationforthefirsttimeinthefamilyLycosidae.Adultfemaleslivefortwoyearsandintheirundergroundburrowstheyproduceonecocoonperseason.TheycarrythecocoonmostlyusinglegsIVandlookafteritforonemonthuntiltheoffspringleave.Maternalcareforspiderlingslastsoneweekfollowingthespiderlings039emergence.date2010sectionpartNumberpartTitleDOI10.1636Hi09-29.1citationKeyurlPMIDPMCIDISSN0161-82021937-2396languageEnglishcollectionsGE86BF5KdateModified2025-11-07T094024ZkeyFFX7XDSSlibraryid5891878metacreatorSummaryMourekandMikoparsedDate2010numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMourekJ.MikoL.ltbgtOntogenyoftheFamulusinSelectedMembersofDamaeidaeAcariOribatidaandItsSuitabilityasaPhylogeneticMarkerltbgt.InltigtTrendsinAcarologyltigtSabelisM.W.BruinJ.Eds.SpringerNetherlandsDordrecht2010pp31x201336.ltaclass039zp-DOIURL039href039httpsdoi.org10.1007978-90-481-9837-5_5039gthttpsdoi.org10.1007978-90-481-9837-5_5ltagt.ltdivgtnltdivgtnltdivgtdataitemTypeconferencePapertitleOntogenyofthefamulusinselectedmembersofDamaeidaeAcariOribatidaanditssuitabilityasaphylogeneticmarkercreatorscreatorTypeauthorfirstNameJanlastNameMourekcreatorTypeauthorfirstNameLadislavlastNameMikocreatorTypeeditorfirstNameMauriceW.lastNameSabeliscreatorTypeeditorfirstNameJanlastNameBruinabstractNoteThefamulusisaspecialisedsetaonthedorsalsideofthetarsusofthefirstpairoflegsinacariformmites.Ithasvariousshapesbutastablelocationwithinthewholegroup.IntheoribatidfamilyDamaeidaetwostatesoffamulusareknownemergentandsunken.Theemergentfamulusisasimpleshortsetawithfullyemergentinsertion.ItispresentintheadultofallknownDamaeidaeandinimmaturestasesofmanydamaeidgenera.Thesunkenfamulusisreducedinsizeandsubmergedinafoveawithanelevatedrimsothatonlyitstipisvisible.InthisstudytheontogeneticdevelopmentofthefamulusinselectedCentralEuropeandamaeidspeciesnamelyDamaeusAdamaeusonustusD.ParadamaeusclavipesEpidamaeustatricusSpatiodamaeusverticillipesKunstidamaeuslengersdorfiandBelbacomptawasstudiedwithlightandscanningelectronmicroscopyandcomparedwiththatofGymnodamaeusbicostatusGymnodamaeidae.LiteraturedataontheontogenyofthefamulusinDamaeidaearesummarizedandthesignificanceofthefamulusasaphylogeneticmarkerisdiscussed.InagreementwithpreviousstudiesadultsofallstudiedmembersofDamaeidaehadanemergentfamulus.TheimmaturesofallstudiedmembersofDamaeussensulatoexceptforE.tatricushadasunkenfamuluswhereastheimmaturesofB.comptahadanemergentfamulus.ImmaturesaswellasadultsofG.bicostatushadasunkenfamulus.IncontrasttoNortonu2019sphylogenetichypothesesallimmaturestasesofE.tatricuspossessedanemergentfamulussimilartoB.compta.ThereforeeitherthemonophylyofEpidamaeusisquestionableormorelikelyreversaltoaplesiomorphicstateoccurredinE.tatricus.proceedingsTitleTrendsinAcarologyconferenceNamedate2010eventPlaceDOI10.1007978-90-481-9837-5_5ISBN978-90-481-9837-5citationKeyurlISSNlanguageencollectionsGE86BF5KdateModified2025-11-07T094851ZkeyT9HPTPMRlibraryid5891878metacreatorSummaryCmejlaetal.parsedDate2010numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtCmejlaR.PtackovaP.PetrakJ.SavvulidiF.CernyJ.SebestaO.VyoralD.ltbgtHumanMRCKAlphaIsRegulatedbyCellularIronLevelsandInterfereswithTransferrinIronUptakeltbgt.ltigtBIOCHEMICALANDBIOPHYSICALRESEARCHCOMMUNICATIONSltigtltbgt2010ltbgtltigt395ltigt2163x2013167.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.bbrc.2010.02.148039gthttpsdoi.org10.1016j.bbrc.2010.02.148ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleHumanMRCKalphaisregulatedbycellularironlevelsandinterfereswithtransferrinironuptakecreatorscreatorTypeauthorfirstNameRadeklastNameCmejlacreatorTypeauthorfirstNamePavlinalastNamePtackovacreatorTypeauthorfirstNameJirilastNamePetrakcreatorTypeauthorfirstNameFilipplastNameSavvulidicreatorTypeauthorfirstNameJanlastNameCernycreatorTypeauthorfirstNameOndrejlastNameSebestacreatorTypeauthorfirstNameDaniellastNameVyoralabstractNoteMyotonicdystrophykinase-relatedCdc42-bindingkinasealphaMRCKalphaformallyknownasCDC42BPAisaserinethreoninekinasethatcanregulateactinmyosinassemblyandactivity.RecentlyithasbeenshownthatitpossessesafunctionalironresponsiveelementIREinthe3039-untranslatedregionUTRofitsmRNAsuggestingthatitmaybeinvolvedinironmetabolism.HerewereportthatMRCKalphaproteinexpressionisalsoregulatedbyironlevelsMRCKalphacolocalizeswithtransferrinTO-loadedtransferrinreceptorsTfRandattenuationofMRCKalphaexpressionbyashorthairpinRNAsilencingconstructleadstoasignificantdecreaseinTf-mediatedironuptake.OurresultsthusindicatethatMRCKalphatakespartinIf-ironuptakeprobablyviaregulationofTf-TfRendocytosisendosometraffickingthatisdependentonthecellularcytoskeleton.RegulationoftheMRCKalphaactivitybyintracellularironlevelscouldthusrepresentanothermolecularfeedbackmechanismcellscouldusetofinelytuneironuptaketoactualneeds.C2010ElsevierInc.Allrightsreserved.date2010sectionpartNumberpartTitleDOI10.1016j.bbrc.2010.02.148citationKeyurlPMIDPMCIDISSN0006-291XlanguagecollectionsGE86BF5KdateModified2025-03-19T115834Zkey64CI3YPClibraryid5891878metacreatorSummaryHuerfanoetal.parsedDate2010numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHuerfanoS.x17DxEDlaV.Boux159aE.x160panielovxE1H.x160tokrovxE1J.ForstovxE1J.ltbgtMinorCapsidProteinsofMousePolyomavirusAreInducersofApoptosisWhenProducedIndividuallybutAreOnlyModerateContributorstoCellDeathduringtheLatePhaseofViralInfectionltbgt.ltigtTheFEBSJournalltigtltbgt2010ltbgtltigt277ltigt51270x20131283.ltaclass039zp-DOIURL039href039httpsdoi.org10.1111j.1742-4658.2010.07558.x039gthttpsdoi.org10.1111j.1742-4658.2010.07558.xltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMinorcapsidproteinsofmousepolyomavirusareinducersofapoptosiswhenproducedindividuallybutareonlymoderatecontributorstocelldeathduringthelatephaseofviralinfectioncreatorscreatorTypeauthorfirstNameSandralastNameHuerfanocreatorTypeauthorfirstNameVojtu011bchlastNameu017du00edlacreatorTypeauthorfirstNameEvu017eenlastNameBouu0159acreatorTypeauthorfirstNameHanalastNameu0160panielovu00e1creatorTypeauthorfirstNameJitkalastNameu0160tokrovu00e1creatorTypeauthorfirstNameJitkalastNameForstovu00e1abstractNoteMinorstructuralproteinsofmousepolyomavirusMPyVareessentialforvirusinfection.TostudytheirpropertiesandpossiblecontributionstocelldeathinductionfusionvariantsoftheseproteinscreatedbylinkingenhancedgreenfluorescentproteinEGFPtotheirC-orN-terminiwerepreparedandtestedintheabsenceofotherMPyVgeneproductsnamelythetumorantigensandthemajorcapsidproteinVP1.TheminorproteinslinkedtoEGFPattheirC-terminusVP2u2013EGFPVP3u2013EGFPwerefoundtodisplaypropertiessimilartotheirnonfusedwild-typeversionstheykilledmouse3T3cellsquicklywhenexpressedindividually.CarryingnuclearlocalizationsignalsattheircommonC-terminustheminorcapsidproteinsweredetectedinthenucleus.HoweverasubstantialsubpopulationofbothVP2andVP3proteinsaswellasofthefusionproteinsVP2u2013EGFPandVP3u2013EGFPwasdetectedinthecytoplasmco-localizingwithintracellularmembranes.TruncatedVP3proteincomposedof103C-terminalaminoacidsexhibitedreducedaffinityforintracellularmembranesandcytotoxicity.Biochemicalstudiesprovedeachoftheminorproteinstobeaverypotentinducerofapoptosiswhichwasdependentoncaspaseactivation.Immuno-electronmicroscopyshowedtheminorproteinstobeassociatedwithdamagedmembranesoftheendoplasmicreticulumnuclearenvelopeandmitochondriaassoonas5hpost-transfection.Analysisofapoptoticmarkersandcelldeathkineticsincellstransfectedwiththewild-typeMPyVgenomeandthegenomemutatedinbothVP2andVP3translationstartcodonsrevealedthattheminorproteinscontributemoderatelytoapoptoticprocessesinthelatephaseofinfectionandbotharedispensableforcelldestructionattheendofthevirusreplicationcycle.Structureddigitalabstractu2022MINT-7386399MINT-7386463MINT-7386515VP3uniprotkbP03096-2andGRP94uniprotkbP08113colocalizeMI0403byfluorescencemicroscopyMI0416u2022MINT-7386328MINT-7386434MINT-7386493VP2uniprotkbP03096-1andGRP94uniprotkbP08113colocalizeMI0403byfluorescencemicroscopyMI0416u2022MINT-7386294MINT-7386413MINT-7386482VP2uniprotkbP03096-1andLamin-BuniprotkbP14733colocalizeMI0403byfluorescencemicroscopyMI0416u2022MINT-7386354MINT-7386450MINT-7386504VP3uniprotkbP12908-2andLamin-BuniprotkbP14733colocalizeMI0403byfluorescencemicroscopyMI0416date2010sectionpartNumberpartTitleDOI10.1111j.1742-4658.2010.07558.xcitationKeyurlhttpsonlinelibrary.wiley.comdoiabs10.1111j.1742-4658.2010.07558.xPMIDPMCIDISSN1742-4658languageencollectionsGE86BF5KdateModified2025-03-07T102510ZkeyYI25LGW3libraryid5891878metacreatorSummaryChanovu00e1etal.parsedDate2009-05-01numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtChanovxE1M.BulantovxE1J.MxE1sloP.HorxE1kP.ltbgtInVitroCultivationofEarlySchistosomulaofNasalandVisceralBirdSchistosomesTrichobilharziaSpp.Schistosomatidaeltbgt.ltigtParasitolResltigtltbgt2009ltbgtltigt104ltigt61445x20131452.ltaclass039zp-ItemURL039href039httpsdoi.org10.1007s00436-009-1343-y039gthttpsdoi.org10.1007s00436-009-1343-yltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleInvitrocultivationofearlyschistosomulaofnasalandvisceralbirdschistosomesTrichobilharziaspp.SchistosomatidaecreatorscreatorTypeauthorfirstNameMartalastNameChanovu00e1creatorTypeauthorfirstNameJanalastNameBulantovu00e1creatorTypeauthorfirstNamePetrlastNameMu00e1slocreatorTypeauthorfirstNamePetrlastNameHoru00e1kabstractNoteCercariaeofbirdschistosomesTrichobilharziaszidatiandTrichobilharziaregentiweremechanicallystimulatedtotransformtoschistosomulaandkeptindifferentcultivationmediasupplementedwithduckredbloodcellsandorhomogenizednervoustissue.Thedevelopmentunderinvitroconditionswascomparedwiththatinvivousingthefollowingcharactersemptyingofpenetrationglandssurfacechangesfooduptakeandgrowthofearlyschistosomula.Theresultsshowthatthecultivationmediumroutinelyusedforhumanschistosomesisalsosuitableformassproductionofearlyschistosomulaofbirdschistosomesincludingtheuniquenasalspeciesu2014T.regenti.Thechangesobservedresemblethosepresentinwormsdevelopinginvivothereforetheinvitroproducedearlyschistosomulamightbeusedforfurtherstudiesofhostu2013parasiteinteractions.date2009-05-01sectionpartNumberpartTitleDOI10.1007s00436-009-1343-ycitationKeyurlhttpsdoi.org10.1007s00436-009-1343-yPMIDPMCIDISSN1432-1955languageencollectionsGE86BF5KdateModified2025-03-10T080252ZkeyPSLUMF3Elibraryid5891878metacreatorSummaryMalenovskyandBurckhardtparsedDate2009-04-29numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMalenovskyI.BurckhardtD.ltbgtAReviewoftheAfrotropicalJumpingPlant-LiceofthePhacopteronidaeHemipteraPsylloidealtbgt.ltigtZootaxaltigtltbgt2009ltbgtNo.20861x201374.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleAreviewoftheAfrotropicaljumpingplant-liceofthePhacopteronidaeHemipteraPsylloideacreatorscreatorTypeauthorfirstNameIgorlastNameMalenovskycreatorTypeauthorfirstNameDaniellastNameBurckhardtabstractNoteTheAfrotropicaljumpingplant-liceofthefamilyPhacopteronidaearerevised.Thirty-onespeciesarerecognized18ofwhicharedescribedasnewPseudophacopteronarcuatumsp.nov.P.aulmannisp.nov.P.benguelensesp.nov.P.bicolorsp.nov.P.carapaesp.nov.P.geminumsp.nov.P.hankaesp.nov.P.hollisisp.nov.P.khayaesp.nov.P.lautererisp.nov.P.magnumsp.nov.P.marmoratumsp.nov.P.nigritulumsp.nov.P.serrifersp.nov.P.sodalissp.nov.P.stigmatumsp.nov.P.tamesseisp.nov.andP.wagnerisp.nov.PseudophacopteroncaffrarienseCapener1973P.pretorienseCapener1973andP.zimmermanniAulmann1912areredescribed.AlectotypeisdesignatedforP.zimmermanni.Allspeciesareillustratedandkeysfortheidentificationofadultsandfifthinstarlarvaeareprovided.Informationisgivenondistributionhostplantsandbiology.Twenty-sixspeciesareassociatedwithplantsoftheorderSapindalesRutalesonespecieshasbeencollectedonTabernaemontanastapfianaGentianalesApocynaceaeandfourspecieslackreliablehostdata.SympatricoccurrencesofseveralPseudophacopteronspeciesonparticularhostspeciesarerecordedonCommiphoraafricanafromKenyaDacryodesedulisfromCameroonEkebergiabenguelensisfromAngolaKhayasenegalensisfromNigeriaandVeprisnobilisfromKenyaandUganda.TwolargeprobablymonophyleticgroupsarerecognizedwithintheAfrotropicalPhacopteronidae.dateAPR292009sectionpartNumberpartTitleDOIcitationKeyurlPMIDPMCIDISSN1175-53261175-5334languageEnglishcollectionsGE86BF5KdateModified2025-11-07T094012Zkey2KXJ3NIBlibraryid5891878metacreatorSummaryHylisetal.parsedDate2007-08numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHylisM.ObornikM.NebesarovaJ.VavraJ.ltbgtAquaticTetrasporoblasticMicrosporidiafromCaddisFliesInsectaTrichopteraCharacterisationPhylogenyandTaxonomicReevaluationoftheGeneraEpiseptumLarsson1986PyrothecaHesse1935andCougourdellaHesse1935ltbgt.ltigtEur.J.Protistol.ltigtltbgt2007ltbgtltigt43ltigt3205x2013224.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.ejop.2007.03.004039gthttpsdoi.org10.1016j.ejop.2007.03.004ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleAquatictetrasporoblasticmicrosporidiafromcaddisfliesInsectaTrichopteraCharacterisationphylogenyandtaxonomicreevaluationofthegeneraEpiseptumLarsson1986PyrothecaHesse1935andCougourdellaHesse1935creatorscreatorTypeauthorfirstNameMiroslavlastNameHyliscreatorTypeauthorfirstNameMiroslavlastNameObornikcreatorTypeauthorfirstNameJanalastNameNebesarovacreatorTypeauthorfirstNameJirilastNameVavraabstractNoteSevenmicrosporidianspeciesinfectingcaddisflylarvaecorrespondingtoconventionalgeneraEpiseptumPyrothecaandCougourdellawerestudiedusinglightandelectronmicroscopy.PartsoftheirsmallsubunitITSandlargesubunitribosomalRNAgenesweresequencedandcomparedwithsequencesofrDNAobtainedfromsyntypeslidesofCougourdellapolycentropiWeiser1965andPyrothecasp.fromHydropsychepellucidula.Allstudiedcaddisflymicrosporidiaformacloselyrelatedgroup.Theirdevelopmentalstagesintrichopteranhostsarerestrictedtofatbodycellsandoenocytesandhaveisolatednuclei.Inlatemerogonyuninucleatemerontsandbinucleateplasmodiaareformed.Insporogonyasporogonialplasmodiumwithfournucleigivesrisebyrosette-likebuddingtofoursporoblastswithinanon-persistentsporophorousvesicle.Sporoblastsmatureintopyriformtolageniformspores.Theshapeandsizeofsporesthenumberofpolarfilamentcoilsthestructureofthepolaroplastandoftheexosporetogetherwithmorphometriccharacterspresentasetofmarkersuniqueforrespectivespecies.Fournewspeciesareestablished.ThenewgenusParaepiseptumisproposedtoreplacethetetrasporoblasticPyrothecaandCougourdellaspeciesfromcaddisflies.ThegenusEpiseptumisredefined.Fieldandlaboratoryexaminationsaswellasthephylogeneticpositionwithintheaquaticcladeofmicrosporidiasuggestthatthelifecycleoftrichopteranmicrosporidiaprobablyinvolvesanalternatecopepodhostandortransovarialtransmission.c2007ElsevierGmbH.Allrightsreserved.dateAUG2007sectionpartNumberpartTitleDOI10.1016j.ejop.2007.03.004citationKeyurlPMIDPMCIDISSN0932-47391618-0429languageEnglishcollectionsGE86BF5KdateModified2025-11-07T094014ZkeyJDN94GMRlibraryid5891878metacreatorSummaryLiebletal.parsedDate2006-05numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtLieblD.DifatoF.HornxEDkovxE1L.MannovxE1P.x160tokrovxE1J.ForstovxE1J.ltbgtMousePolyomavirusEntersEarlyEndosomesRequiresTheirAcidicpHforProductiveInfectionandMeetsTransferrinCargoinRab11-PositiveEndosomesltbgt.ltigtJournalofVirologyltigtltbgt2006ltbgtltigt80ltigt94610x20134622.ltaclass039zp-DOIURL039href039httpsdoi.org10.1128jvi.80.9.4610-4622.2006039gthttpsdoi.org10.1128jvi.80.9.4610-4622.2006ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleMousePolyomavirusEntersEarlyEndosomesRequiresTheirAcidicpHforProductiveInfectionandMeetsTransferrinCargoinRab11-PositiveEndosomescreatorscreatorTypeauthorfirstNameDavidlastNameLieblcreatorTypeauthorfirstNameFrancescolastNameDifatocreatorTypeauthorfirstNameLenkalastNameHornu00edkovu00e1creatorTypeauthorfirstNamePetralastNameMannovu00e1creatorTypeauthorfirstNameJitkalastNameu0160tokrovu00e1creatorTypeauthorfirstNameJitkalastNameForstovu00e1abstractNoteMousepolyomavirusPyVvirionsentercellsbyinternalizationintosmoothmonopinocyticvesicleswhichfuseunderthecellmembranewithlargerendosomes.Caveolin-1wasdetectedonmonopinocyticvesiclescarryingPyVparticlesinmousefibroblastsandepithelialcells33.HereweshowthatPyVcanbeefficientlyinternalizedbyJurkatcellswhichdonotexpresscaveolin-1andlackcaveolaeandthatoverexpressionofacaveolin-1dominant-negativemutantinmouseepithelialcellsdoesnotpreventtheirproductiveinfection.StrongcolocalizationofVP1withearlyendosomeantigen1EEA1andofEEA1withcaveolin-1inmousefibroblastsandepithelialcellssuggeststhatthemonopinocyticvesiclescarryingthevirusandvesiclescontainingcaveolin-1fusewithEEA1-positiveearlyendosomes.IncontrasttoSV40PyVinfectionisdependentontheacidicpHofendosomes.BafilomycinA1abolishedPyVinfectionandanincreaseinendosomalpHbyNH4Clmarkedlyreduceditsefficiencywhendrugswereappliedduringviriontransporttowardsthecellnucleus.Theblockofacidificationresultedintheretentionofafractionofvirionsinearlyendosomes.TomonitorfurthertraffickingofPyVweusedfluorescentresonanceenergytransferFRETtodeterminemutuallocalizationofPyVVP1withtransferrinandRab11GTPaseata2-to10-nmresolution.PositiveFRETbetweenPyVVP1andtransferrincargoandbetweenPyVVP1andRab11suggeststhatduringlatertimespostinfection1.5to3hthevirusmeetsupwithtransferrinintheRab11-positiverecyclingendosome.Theseresultspointtoaconvergenceofthevirusandthecargointernalizedbydifferentpathwaysincommontransitionalcompartments.date2006-05sectionpartNumberpartTitleDOI10.1128jvi.80.9.4610-4622.2006citationKeyurlhttpsjournals.asm.orgdoi10.1128jvi.80.9.4610-4622.2006PMIDPMCIDISSNlanguagecollectionsGE86BF5KdateModified2025-03-07T102608ZkeyR24Z6BK2libraryid5891878metacreatorSummaryHyliu0161etal.parsedDate2006numChildren0bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHylix161M.PilarskaD.K.ObornxEDkM.VxE1vraJ.SolterL.F.WeiserJ.LindeA.McManusM.L.ltbgtNosemaChrysorrhoeaen.Sp.MicrosporidiaIsolatedfromBrowntailMothEuproctisChrysorrhoeaL.LepidopteraLymantriidaeinBulgariaCharacterizationandPhylogeneticRelationshipsltbgt.ltigtJournalofInvertebratePathologyltigtltbgt2006ltbgtltigt91ltigt2105x2013114.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.jip.2005.11.006039gthttpsdoi.org10.1016j.jip.2005.11.006ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleNosemachrysorrhoeaen.sp.MicrosporidiaisolatedfrombrowntailmothEuproctischrysorrhoeaL.LepidopteraLymantriidaeinBulgariaCharacterizationandphylogeneticrelationshipscreatorscreatorTypeauthorfirstNameMiroslavlastNameHyliu0161creatorTypeauthorfirstNameDanielaK.lastNamePilarskacreatorTypeauthorfirstNameMiroslavlastNameObornu00edkcreatorTypeauthorfirstNameJiu0159u00edlastNameVu00e1vracreatorTypeauthorfirstNameLeellenF.lastNameSoltercreatorTypeauthorfirstNameJaroslavlastNameWeisercreatorTypeauthorfirstNameAndreaslastNameLindecreatorTypeauthorfirstNameMichaelL.lastNameMcManusabstractNotedate22006sectionpartNumberpartTitleDOI10.1016j.jip.2005.11.006citationKeyurlhttpslinkinghub.elsevier.comretrievepiiS0022201105002430PMIDPMCIDISSN00222011languageencollectionsGE86BF5KdateModified2025-11-07T094704ZkeyYPH6WDKClibraryid5891878metacreatorSummaryMraceketal.parsedDate2006numChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtMracekT.PecinaP.VojtiskovaA.KalousM.SebestaO.HoustekJ.ltbgtTwoComponentsinPathogenicMechanismofMitochondrialATPaseDeficiencyEnergyDeprivationandROSProductionltbgt.ltigtEXPERIMENTALGERONTOLOGYltigtltbgt2006ltbgtltigt41ltigt7683x2013687.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.exger.2006.02.009039gthttpsdoi.org10.1016j.exger.2006.02.009ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleTwocomponentsinpathogenicmechanismofmitochondrialATPasedeficiencyEnergydeprivationandROSproductioncreatorscreatorTypeauthorfirstNameTomaslastNameMracekcreatorTypeauthorfirstNamePetrlastNamePecinacreatorTypeauthorfirstNameAlenalastNameVojtiskovacreatorTypeauthorfirstNameMartinlastNameKalouscreatorTypeauthorfirstNameOndrejlastNameSebestacreatorTypeauthorfirstNameJoseflastNameHoustekabstractNoteIsolateddefectsofmitochondrialATPaseduetodiminishedbiosynthesisoftheenzymerepresentnewclassofseveremitochondrialdiseasesofnuclearorigin.TheprimarycauseofdecreasedcellularcontentofATPaseappearstobeaprobleminassemblyoftheF-1catalyticpartoftheenzyme.WiththeaimtoelucidatehowthelowATPasecontentaffectsmitochondrialenergyprovisionandROSproductionwehaveinvestigatedfibroblastsfrompatientswithATPasedecreaseto10-30.MeasurementsOfcellularrespirationshowedpronounceddecreaseinATPasecapacityforbasalrespirationmitochondrialATPsynthesiswasdecreasedto26-33.CytofluorometricanalysisusingTMRMrevealedaltereddischargeofmitochondrialmembranepotentialDeltapsiminpatientcellswhichwas20mVincreasedatstate3-ADP.AnalysisofROSproductionbyCM-H2DCFDAdemonstrated2-foldincreaseinROSproductioninpatientcellscomparedtocontrols.ROSproductionratewassensitivetouncouplerFCCPandthusapparentlyrelatedtoincreasedDeltapsim.OurstudiesclearlydemonstratethatlowATPasecontentanddecreasedrnitochondrialATPproductionleadtohighvaluesofDeltapsimandareassociatedwithactivationofROSgenerationbythemitochondrialrespiratorychain.InconclusionboththeenergeticdeprivationandincreasedoxidativestressareimportantcomponentsofthepathogenicmechanismofATPasedisorders.c2006ElsevierInc.Allrightsreserved.date2006sectionpartNumberpartTitleDOI10.1016j.exger.2006.02.009citationKeyurlPMIDPMCIDISSN0531-5565languagecollectionsGE86BF5KdateModified2025-03-19T115833ZkeyZZNQ8C7Rlibraryid5891878metacreatorSummaryHyliu0161etal.parsedDate2005-03-01numChildren2bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtHylix161M.WeiserJ.ObornxEDkM.VxE1vraJ.ltbgtDNAIsolationfromMuseumandTypeCollectionSlidesofMicrosporidialtbgt.ltigtJournalofInvertebratePathologyltigtltbgt2005ltbgtltigt88ltigt3257x2013260.ltaclass039zp-DOIURL039href039httpsdoi.org10.1016j.jip.2005.02.004039gthttpsdoi.org10.1016j.jip.2005.02.004ltagt.ltdivgtnltdivgtnltdivgtdataitemTypejournalArticletitleDNAisolationfrommuseumandtypecollectionslidesofmicrosporidiacreatorscreatorTypeauthorfirstNameMiroslavlastNameHyliu0161creatorTypeauthorfirstNameJaroslavlastNameWeisercreatorTypeauthorfirstNameMiroslavlastNameObornu00edkcreatorTypeauthorfirstNameJiu0159u00edlastNameVu00e1vraabstractNoteDNAfrom19speciesofmicrosporidiawasisolatedandamplifiedfrominfectedhosttissuethatwereoriginallypreparedbetweentheyears1946and1996.ThesmearsonglassmicroscopeslideswereeitherGiemsa-stainedorunstained.Methanol-fixedGiemsa-stainedsmearsprovedtobesuitableforDNAisolationDNAwasamplifiedfromonlytwoof14unstainedslides.TheisolatedDNAwassuccessfullyamplifiedinPCRsusingsmallsubunitandlargesubunitrDNAprimersandsequenced.ThehighefficiencyofDNAisolationdemonstratestheusefulnessofarchivalandtypecollectionslidesforsomemolecularbiologyandmoleculartaxonomystudies.date2005-03-01sectionpartNumberpartTitleDOI10.1016j.jip.2005.02.004citationKeyurlhttpswww.sciencedirect.comsciencearticlepiiS0022201105000479PMIDPMCIDISSN0022-2011languagecollectionsGE86BF5KdateModified2025-11-07T094510ZkeyGAPFLKR5libraryid5891878metanumChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtltigtNosemaraphidiaesp.n.MicrosporidaNosematidaeAMicrosporidianPathogenofthePredatorySnake-flyRaphidiaophiopsisRaphidiopteraRaphidiidae-WebofScienceCoreCollectionltigt.ltaclass039zp-ItemURL039href039httpswww.webofscience.comwoswosccfull-recordWOS000274737300007039gthttpswww.webofscience.comwoswosccfull-recordWOS000274737300007ltagtaccessed2025-11-07.ltdivgtnltdivgtnltdivgtdataitemTypewebpagetitleNosemaraphidiaesp.n.MicrosporidaNosematidaeAMicrosporidianPathogenofthePredatorySnake-flyRaphidiaophiopsisRaphidiopteraRaphidiidae-WebofScienceCoreCollectioncreatorsabstractNotedateDOIcitationKeyurlhttpswww.webofscience.comwoswosccfull-recordWOS000274737300007languagecollectionsGE86BF5KdateModified2025-11-07T094710ZkeyKNTLVFIGlibraryid5891878metanumChildren1bibltdivclassquotcsl-bib-bodyquotstylequotline-height1.35quotgtnltdivclassquotcsl-entryquotstylequotclearleftquotgtnltdivclassquotcsl-left-marginquotstylequotfloatleftpadding-right0.5emtext-alignrightwidth1emquotgt1.ltdivgtltdivclassquotcsl-right-inlinequotstylequotmargin0.4em01.5emquotgtltigtNewrecordsandothernotesforOregonColeoptera-WebofScienceCoreCollectionltigt.ltaclass039zp-ItemURL039href039httpswww.webofscience.comwoswosccfull-recordWOS000235856600001039gthttpswww.webofscience.comwoswosccfull-recordWOS000235856600001ltagtaccessed2025-11-07.ltdivgtnltdivgtnltdivgtdataitemTypewebpagetitleNewrecordsandothernotesforOregonColeoptera-WebofScienceCoreCollectioncreatorsabstractNotedateDOIcitationKeyurlhttpswww.webofscience.comwoswosccfull-recordWOS000235856600001languagecollectionsGE86BF5KdateModified2025-11-07T094654Z
1.
Vavra, J.; Hylis, M.; Fiala, I.; Nebesarova, J. Globulispora Mitoportans n. g., n. Sp., (Opisthosporidia: Microsporidia) a Microsporidian Parasite of Daphnids with Unusual Spore Organization and Prominent Mitosome-like Vesicles. J. Invertebr. Pathol. 2016, 135, 43–52. https://doi.org/10.1016/j.jip.2016.02.003.
1.
Vavra, J.; Hylis, M.; Fiala, I.; Refardt, D.; Larsson, J. I. R. Microsporidia in a Woodland Pool I. Lanatospora Costata Sp n. (Opisthosporidia, Microsporidia), Parasite of Megacyclops Viridis (Crustacea, Copepoda): Fine Structure and Molecular Phylogeny. Acta Protozool. 2016, 55 (4), 269–280. https://doi.org/10.4467/16890027AP.16.023.6010.
1.
Rohanova, M.; Schaefer, C. W.; Krizkova, P.; Vilimova, J. Scent Efferent System of Dorsal Abdominal Scent Glands in Nymphs of Rhopalidae (Hemiptera: Heteroptera: Pentatomomorpha) and Its Comparison with Other Pentatomomorpha. Zool. Anz. 2016, 260, 1–10. https://doi.org/10.1016/j.jcz.2015.11.001.
1.
Lankova, M.; Humpolickova, J.; Vosolsobe, S.; Cit, Z.; Lacek, J.; Covan, M.; Covanova, M.; Hof, M.; Petrasek, J. Determination of Dynamics of Plant Plasma Membrane Proteins with Fluorescence Recovery and Raster Image Correlation Spectroscopy. MICROSCOPY AND MICROANALYSIS 2016, 22 (2), 290–299. https://doi.org/10.1017/S1431927616000568.
1.
Smrz, J.; Soukalova, H.; Catska, V.; Hubert, J. Feeding Patterns of Tyrophagus Putrescentiae (Sarcoptiformes: Acaridae) Indicate That Mycophagy Is Not a Single and Homogeneous Category of Nutritional Biology. JOURNAL OF INSECT SCIENCE 2016, 16. https://doi.org/10.1093/jisesa/iew070.
1.
Micova, P.; Hahnova, K.; Hlavackova, M.; Elsnicova, B.; Chytilova, A.; Holzerova, K.; Zurmanova, J.; Neckar, J.; Kolar, F.; Novakova, O.; Novotny, J. Chronic Intermittent Hypoxia Affects the Cytosolic Phospholipase A(2)Alpha/Cyclooxygenase 2 Pathway via Beta(2)-Adrenoceptor-Mediated ERK/P38 Stimulation. MOLECULAR AND CELLULAR BIOCHEMISTRY 2016, 423 (1–2), 151–163. https://doi.org/10.1007/s11010-016-2833-8.
1.
Hahnova, K.; Pacesova, D.; Volfova, B.; Cervena, K.; Kasparova, D.; Zurmanova, J.; Bendova, Z. Circadian Dexras 1 in Rats: Development, Location and Responsiveness to Light. CHRONOBIOLOGY INTERNATIONAL 2016, 33 (2), 141–150. https://doi.org/10.3109/07420528.2015.1120741.
1.
Flieger, M.; Bandouchova, H.; Cerny, J.; Chudickova, M.; Kolarik, M.; Kovacova, V.; Martinkova, N.; Novak, P.; Sebesta, O.; Stodulkova, E.; Pikula, J. Vitamin B-2 as a Virulence Factor in Pseudogymnoascus Destructans Skin Infection. SCIENTIFIC REPORTS 2016, 6. https://doi.org/10.1038/srep33200.
1.
Melkes, B.; Hejnova, L.; Novotny, J. Biased Mu-Opioid Receptor Agonists Diversely Regulate Lateral Mobility and Functional Coupling of the Receptor to Its Cognate G Proteins. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY 2016, 389 (12), 1289–1300. https://doi.org/10.1007/s00210-016-1293-8.
1.
Jurjevic, Z.; Kubatova, A.; Kolarik, M.; Hubka, V. Taxonomy of Aspergillus Section Petersonii Sect. Nov Encompassing Indoor and Soil-Borne Species with Predominant Tropical Distribution. Plant Syst. Evol. 2015, 301 (10), 2441–2462. https://doi.org/10.1007/s00606-015-1248-4.
1.
Hubka, V.; Nissen, C. V.; Jensen, R. H.; Arendrup, M. C.; Cmokova, A.; Kubatova, A.; Skorepova, M.; Kolarik, M. Discovery of a Sexual Stage in Trichophyton Onychocola, a Presumed Geophilic Dermatophyte Isolated from Toenails of Patients with a History of T. Rubrum Onychomycosis. Med. Mycol. 2015, 53 (8), 798–809. https://doi.org/10.1093/mmy/myv044.
1.
Nemcova, Y.; Pichrtova, M.; Zeisek, V. Mallomonas Alpestrina Sp. Nov. (Synurales, Chrysophyceae, Stramenopiles) and Its Spineless Relatives—Mallomonas Alata Group. Phytotaxa 2015, 222 (2), 111. https://doi.org/10.11646/phytotaxa.222.2.3.
1.
Hernychova, L.; Mrazek, H.; Grobarova, V.; Kukacka, Z.; Sebesta, O.; Cerny, J.; Novak, P. Structural and Functional Characterization of the Mouse Inhibitory C-Type Lectin-like Receptor. FEBS J. 2015, 282, 341–342.
1.
Dolejs, P.; Buchar, J.; Kubcova, L.; Smrz, J. Developmental Changes in the Spinning Apparatus over the Life Cycle of Wolf Spiders (Araneae: Lycosidae). Invertebr. Biol. 2014, 133 (3), 281–297. https://doi.org/10.1111/ivb.12055.
1.
Zila, V.; Difato, F.; Klimova, L.; Huerfano, S.; Forstova, J. Involvement of Microtubular Network and Its Motors in Productive Endocytic Trafficking of Mouse Polyomavirus. PLOS ONE 2014, 9 (5), e96922. https://doi.org/10.1371/journal.pone.0096922.
1.
Bartosik, A. A.; Glabski, K.; Jecz, P.; Lasocki, K.; Mikosa, M.; Plochocka, D.; Thomas, C. M.; Jagura-Burdzy, G. Dissection of the Region of Pseudomonas Aeruginosa ParA That Is Important for Dimerization and Interactions with Its Partner ParB. MICROBIOLOGY-SGM 2014, 160, 2406–2420. https://doi.org/10.1099/mic.0.081216-0.
1.
Purkartova, Z.; Tuma, J.; Pesta, M.; Kulda, V.; Hajkova, L.; Sebesta, O.; Vozeh, F.; Cendelin, J. Morphological Analysis of Embryonic Cerebellar Grafts in SCA2 Mice. NEUROSCIENCE LETTERS 2014, 558, 154–158. https://doi.org/10.1016/j.neulet.2013.11.020.
1.
Hylis, M.; Obornik, M.; Nebesarova, J.; Vavra, J. Description and Phylogeny of Zelenkaia Trichopterae Gen. et Sp Nov (Microsporidia), an Aquatic Microsporidian Parasite of Caddisflies (Trichoptera) Forming Spore Doublets. J. Invertebr. Pathol. 2013, 114 (1), 11–21. https://doi.org/10.1016/j.jip.2013.04.010.
1.
Hubka, V.; Dobiasova, S.; Lyskova, P.; Mallatova, N.; Chlebkova, J.; Skorepova, M.; Kubatova, A.; Dobias, R.; Chudickova, M.; Kolarik, M. Auxarthron Ostraviense Sp Nov., and A. Umbrinum Associated with Non-Dermatophytic Onychomycosis. Med. Mycol. 2013, 51 (6), 614–624. https://doi.org/10.3109/13693786.2013.770608.
1.
Kutalova, K.; Bourguignon, T.; Sillam-Dusses, D.; Hanus, R.; Roisin, Y.; Sobotnik, J. Armed Reproductives: Evolution of the Frontal Gland in Imagoes of Termitidae. Arthropod Struct. Dev. 2013, 42 (4), 339–348. https://doi.org/10.1016/j.asd.2013.04.001.
1.
Forman, M.; Nguyen, P.; Hula, V.; Král, J. Sex Chromosome Pairing and Extensive NOR Polymorphism in Wadicosa Fidelis (Araneae: Lycosidae). Cytogenetic and Genome Research 2013, 141 (1), 43–49. https://doi.org/10.1159/000351041.
1.
Hubka, V.; Peterson, S. W.; Frisvad, J. C.; Yaguchi, T.; Kubatova, A.; Kolarik, M. Aspergillus Waksmanii Sp Nov and Aspergillus Marvanovae Sp Nov., Two Closely Related Species in Section Fumigati. Int. J. Syst. Evol. Microbiol. 2013, 63, 783–789. https://doi.org/10.1099/ijs.0.047076-0.
1.
Vávra, J.; Hyliš, M.; Oborník, M.; Vossbrinck, C. R. Microsporidia in Aquatic Microcrustacea: The Copepod Microsporidium Marssoniella Elegans Lemmermann, 1900 Revisited. Folia Parasitologica 2013, 52 (1–2), 163–172. https://doi.org/10.14411/fp.2005.021.
1.
Huerfano, S.; Ryabchenko, B.; Forstová, J. Nucleofection of Expression Vectors Induces a Robust Interferon Response and Inhibition of Cell Proliferation. DNA and Cell Biology 2013, 32 (8), 467–479. https://doi.org/10.1089/dna.2012.1950.
1.
Waskova-Arnostova, P.; Elsnicova, B.; Kasparova, D.; Sebesta, O.; Novotny, J.; Neckar, J.; Kolar, F.; Zurmanova, J. Right-To-Left Ventricular Differences in the Expression of Mitochondrial Hexokinase and Phosphorylation of Akt. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 2013, 31 (1), 66–79. https://doi.org/10.1159/000343350.
1.
Jindrová, A.; Tůma, J.; Sládek, V. Intra-Observer Error of Mouse Long Bone Cross Section Digitization. fozo 2012, 61 (3–4), 340–349. https://doi.org/10.25225/fozo.v61.i3.a1.2012.
1.
Vilímová, J.; Kutalová, K. Occurrence of Certain Cuticular Structures Confirms Functionality of Dorsal Abdominal Scent Glands in Acanthosomatidae (Heteroptera: Pentatomoidea). Bull. Entomol. Res. 2012, 102 (1), 29–42. https://doi.org/10.1017/S0007485311000344.
1.
Pažoutová, S.; Šrůtka, P.; Holuša, J.; Chudíčková, M.; Kubátová, A.; Kolařík, M. Liberomyces Gen. Nov. with Two New Species of Endophytic Coelomycetes from Broadleaf Trees. Mycologia 2012, 104 (1), 198–210. https://doi.org/10.3852/11-081.
1.
Dejmkova, H.; Zima, J.; Barek, J.; Mika, J. Behavior of Glassy Carbon Paste Electrode in Flowing Methanolic Solutions. ELECTROANALYSIS 2012, 24 (8), 1766–1770. https://doi.org/10.1002/elan.201100598.
1.
Horníková, L.; Man, P.; Forstová, J. Blue Native Protein Electrophoresis for Studies of Mouse Polyomavirus Morphogenesis and Interactions between the Major Capsid Protein VP1 and Cellular Proteins. Journal of Virological Methods 2011, 178 (1), 229–234. https://doi.org/10.1016/j.jviromet.2011.08.019.
1.
Bulantová, J.; Chanová, M.; Houžvičková, L.; Horák, P. Trichobilharzia Regenti (Digenea: Schistosomatidae): Changes of Body Wall Musculature during the Development from Miracidium to Adult Worm. Micron 2011, 42 (1), 47–54. https://doi.org/10.1016/j.micron.2010.08.003.
1.
Conrad, M.; Zubacova, Z.; Dunn, L. A.; Upcroft, J.; Sullivan, S. A.; Tachezy, J.; Carlton, J. M. Microsatellite Polymorphism in the Sexually Transmitted Human Pathogen Trichomonas Vaginalis Indicates a Genetically Diverse Parasite. Molecular and Biochemical Parasitology 2011, 175 (1), 30–38. https://doi.org/10.1016/j.molbiopara.2010.08.006.
1.
Kepkova, K. V.; Vodicka, P.; Toralova, T.; Lopatarova, M.; Cech, S.; Dolezel, R.; Havlicek, V.; Besenfelder, U.; Kuzmany, A.; Sirard, M. A.; Laurincik, J.; Kanka, J. Transcriptomic Analysis of in Vivo a in Vitro Produced Bovine Embryos Revealed a Developmental Change in Cullin 1 Expression during Maternal-to-Embryonic Transition. THERIOGENOLOGY 2011, 75 (9), 1582–1595. https://doi.org/10.1016/j.theriogenology.2010.12.019.
1.
Ligasova, A.; Bulantova, J.; Sebesta, O.; Kasny, M.; Koberna, K.; Mikes, L. Secretory Glands in Cercaria of the Neuropathogenic Schistosome Trichobilharzia Regenti - Ultrastructural Characterization, 3-D Modelling, Volume and pH Estimations. PARASITES & VECTORS 2011, 4. https://doi.org/10.1186/1756-3305-4-162.
1.
Doubravska, L.; Krausova, M.; Gradl, D.; Vojtechova, M.; Tumova, L.; Lukas, J.; Valenta, T.; Pospichalova, V.; Fafilek, B.; Plachy, J.; Sebesta, O.; Korinek, V. Fatty Acid Modification of Wnt1 and Wnt3a at Serine Is Prerequisite for Lipidation at Cysteine and Is Essential for Wnt Signalling. CELLULAR SIGNALLING 2011, 23 (5), 837–848. https://doi.org/10.1016/j.cellsig.2011.01.007.
1.
Juracka, P. J.; Korinek, V.; Petrusek, A. A New Central European Species of the Daphnia Curvirostris Complex, Daphnia Hrbaceki Sp Nov (Cladocera, Anomopoda, Daphniidae). Zootaxa 2010, No. 2718, 1–22.
1.
Vrkoslav, V.; Muck, A.; Cvačka, J.; Svatoš, A. MALDI Imaging of Neutral Cuticular Lipids in Insects and Plants. J. Am. Soc. Mass Spectrom. 2010, 21 (2), 220–231. https://doi.org/10.1016/j.jasms.2009.10.003.
1.
Dolejs, P.; Kubcova, L.; Buchar, J. Courtship, Mating, and Cocoon Maintenance of Tricca Lutetiana (Araneae: Lycosidae). J. Arachnol. 2010, 38 (3), 504–510. https://doi.org/10.1636/Hi09-29.1.
1.
Mourek, J.; Miko, L. Ontogeny of the Famulus in Selected Members of Damaeidae (Acari: Oribatida) and Its Suitability as a Phylogenetic Marker. In Trends in Acarology; Sabelis, M. W., Bruin, J., Eds.; Springer Netherlands: Dordrecht, 2010; pp 31–36. https://doi.org/10.1007/978-90-481-9837-5_5.
1.
Cmejla, R.; Ptackova, P.; Petrak, J.; Savvulidi, F.; Cerny, J.; Sebesta, O.; Vyoral, D. Human MRCK Alpha Is Regulated by Cellular Iron Levels and Interferes with Transferrin Iron Uptake. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2010, 395 (2), 163–167. https://doi.org/10.1016/j.bbrc.2010.02.148.
1.
Huerfano, S.; Žíla, V.; Bouřa, E.; Španielová, H.; Štokrová, J.; Forstová, J. Minor Capsid Proteins of Mouse Polyomavirus Are Inducers of Apoptosis When Produced Individually but Are Only Moderate Contributors to Cell Death during the Late Phase of Viral Infection. The FEBS Journal 2010, 277 (5), 1270–1283. https://doi.org/10.1111/j.1742-4658.2010.07558.x.
1.
Chanová, M.; Bulantová, J.; Máslo, P.; Horák, P. In Vitro Cultivation of Early Schistosomula of Nasal and Visceral Bird Schistosomes (Trichobilharzia Spp., Schistosomatidae). Parasitol Res 2009, 104 (6), 1445–1452. https://doi.org/10.1007/s00436-009-1343-y.
1.
Malenovsky, I.; Burckhardt, D. A Review of the Afrotropical Jumping Plant-Lice of the Phacopteronidae (Hemiptera: Psylloidea). Zootaxa 2009, No. 2086, 1–74.
1.
Hylis, M.; Obornik, M.; Nebesarova, J.; Vavra, J. Aquatic Tetrasporoblastic Microsporidia from Caddis Flies (Insecta, Trichoptera):: Characterisation, Phylogeny and Taxonomic Reevaluation of the Genera Episeptum Larsson, 1986, Pyrotheca Hesse, 1935 and Cougourdella Hesse, 1935. Eur. J. Protistol. 2007, 43 (3), 205–224. https://doi.org/10.1016/j.ejop.2007.03.004.
1.
Liebl, D.; Difato, F.; Horníková, L.; Mannová, P.; Štokrová, J.; Forstová, J. Mouse Polyomavirus Enters Early Endosomes, Requires Their Acidic pH for Productive Infection, and Meets Transferrin Cargo in Rab11-Positive Endosomes. Journal of Virology 2006, 80 (9), 4610–4622. https://doi.org/10.1128/jvi.80.9.4610-4622.2006.
1.
Hyliš, M.; Pilarska, D. K.; Oborník, M.; Vávra, J.; Solter, L. F.; Weiser, J.; Linde, A.; McManus, M. L. Nosema Chrysorrhoeae n. Sp. (Microsporidia), Isolated from Browntail Moth (Euproctis Chrysorrhoea L.) (Lepidoptera, Lymantriidae) in Bulgaria: Characterization and Phylogenetic Relationships. Journal of Invertebrate Pathology 2006, 91 (2), 105–114. https://doi.org/10.1016/j.jip.2005.11.006.
1.
Mracek, T.; Pecina, P.; Vojtiskova, A.; Kalous, M.; Sebesta, O.; Houstek, J. Two Components in Pathogenic Mechanism of Mitochondrial ATPase Deficiency: Energy Deprivation and ROS Production. EXPERIMENTAL GERONTOLOGY 2006, 41 (7), 683–687. https://doi.org/10.1016/j.exger.2006.02.009.
1.
Hyliš, M.; Weiser, J.; Oborník, M.; Vávra, J. DNA Isolation from Museum and Type Collection Slides of Microsporidia. Journal of Invertebrate Pathology 2005, 88 (3), 257–260. https://doi.org/10.1016/j.jip.2005.02.004.
1.
Nosema raphidiae sp.n. (Microsporida, Nosematidae): A Microsporidian Pathogen of the Predatory Snake-fly Raphidia ophiopsis (Raphidioptera: Raphidiidae)-Web of Science Core Collection. https://www.webofscience.com/wos/woscc/full-record/WOS:000274737300007 (accessed 2025-11-07).
1.
New records and other notes for Oregon Coleoptera-Web of Science Core Collection. https://www.webofscience.com/wos/woscc/full-record/WOS:000235856600001 (accessed 2025-11-07).