Tomáš Křížek
Department of Analytical Chemistry, Door #118
tel. +420 221 951 229
e-mail: tomas.krizek@natur.cuni.cz
consultation hours: flexible, after previous agreement via e-mail.
TEACHING
Advances in Separation Science
Analytical Chemistry I (a)
Analytical Chemistry II (a)
Electromigration methods
Laboratory Course on Separation Methods
Chemical Calculations
Presenting and Public Speaking
STUDY MATERIALS
Advances in separation science
Capillary electrophoresis enzyme assays
Sample stacking techniques in capillary electrophoresis
Comprehensive gas chromatography
MIGHT BE INTERESTING FOR YOU
Toastmasters - club focused on public speaking and development of communication and leadership skills in English.
RESEARCH INTERESTS
Capillary electrophoresis in investigation of intermolecular interactions, enzyme reactions, and controlled drug release studies.
Application of separation techniques in combination with electrochemistry for stability and forced degradation studies of pharmaceutical substances.
Applications of capillary electrophoresis for biochemical, biological, and medical research.
PAPERS
ResearcherID L-8349-2017 , ORCID 0000-0002-1623-8844, Scopus Author ID 54936071100, Publons
Šimonová A., Balouch M., Štěpánek F., Křížek T.:
Investigating drug-liposome interactions using liposomal electrokinetic chromatography.
Analytical and Bioanalytical Chemistry 417 (2025) 2029-2038. DOI: 10.1007/s00216-025-05783-6
Frejková M., Behalová K., Rubanová D., De Sanctis J. B., Kubala L., Chytil P., Šimonová A., Křížek T., Randárová E., Gunár K., Etrych T.:
Polymer nanotherapeutics with the controlled release of acetylsalicylic acid and its derivatives inhibiting cyclooxygenase isoforms and reducing the production of pro-inflammatory mediators.
International Journal of Pharmaceutics 665 (2024) 124742. DOI: 10.1016/j.ijpharm.2024.124742
Dushkov A., Vosáhlová Z., Tzintzarov A., Kalíková K., Křížek T., Ugrinova I:
Analysis of the Ibotenic Acid, Muscimol, and Ergosterol Content of an Amanita Muscaria Hydroalcoholic Extract with an Evaluation of Its Cytotoxic Effect against a Panel of Lung Cell Lines In Vitro.
Molecules 28 (2023) 6824. DOI: 10.3390/molecules28196824
Hýsková V., Jákl M., Dytrtová J.J., Zeljkovic S. C., Vrobel O., Bělonožníková K., Kavan D., Křížek T., Šimonová A., Vašková M., Kovac I., Zufic A. R., Ryšlavá H.:
Triazoles as a Potential Threat to the Nutritional Quality of Tomato Fruits.
Metabolites 13 (2023) 988. DOI: 10.3390/metabo13090988
Libánská A., Špringer T., Peštová L, Kotalík K., Konefal R., Šimonová A., Křížek T., Homola J., Randárová E., Etrych T.:
Using surface plasmon resonance, capillary electrophoresis and diffusion-ordered NMR spectroscopy to study drug release kinetics.
Communications Chemistry 6 (2023) 180. DOI: 10.1038/s42004-023-00992-5
Vlčková N., Šimonová A., Duriš M., Čokrtová K., Almquist S, Křížek T.:
Detection techniques for carbohydrates in capillary electrophoresis - a comparative study.
Monatshefte für Chemie - Chemical Monthly 154 (2023) 967-975. DOI: 10.1007/s00706-023-03109-9
Bělonožníková K., Černý M., Hýsková V., Synková H., Valcke R., Hodek O., Křížek T., Kavan D., Vaňková R., Dobrev P., Haisel D., Ryšlavá H:
Casein as protein and hydrolysate: Biostimulant or nitrogen source for Nicotiana tabacum plants grown in vitro?
Physiologia Plantarum 175 (2023) e13973. DOI: 10.1111/ppl.13973
Kožich V., Schwahn B. C., Sokolová J., Křížková M., Ditroi T., Krijt J., Khalil Y., Křížek T., Vaculíková-Fantlová T., Stibůrková B., Mills P., Clayton P., Barvíková K., Blessing H., Sykut-Cegielska J., Dionisi-Vici C., Gasperini S., García-Cazorla A., Haack T. B., Honzík T., Ješina P., Kuster A., Laugwitz L., Martinelli D., Porta F., Santer R., Schwart G., Nagy P.:
Human ultrarare genetic disorders of sulfur metabolism demonstrate redundancies in H2S homeostasis.
Redox Biology 58 (2022) 102517. DOI: 10.1016/j.redox.2022.102517
Tomnikova A., Kozlík P., Křížek T.:
Monosaccharide profiling of glycoproteins by capillary electrophoresis with contactless conductivity detection.
Electrophoresis 43 (2022) 1963-1970. DOI: 10.1002/elps.202200033
Tomnikova A., Orgoníková A., Křížek T.:
Liposomes: preparation and characterization with a special focus on the application of capillary electrophoresis.
Monatshefte für Chemie - Chemical Monthly 153 (2022) 687-695. DOI: 10.1007/s00706-022-02966-0
Do Pham D. D., Mojr V., Helusová M., Mikušová G., Pohl R., Dávidová E., Šanderová H., Vítovská D., Bogdanová K., Večeřová R., Sedláková M. H., Fišer R., Sudzinová P., Galandáková A., Kolár M., Krásný L., Rejman D.:
LEGO-Lipophosphonoxins: A Novel Approach in Designing Membrane Targeting Antimicrobials.
Journal of Medicinal Chemistry 65 (2022) 10045-10078. DOI: 10.1021/acs.jmedchem.2c00684
Bělonožníková K., Hýsková V., Vašková M., Křížek T., Čokrtová K., Vanět T., Halířová L., Chudý M., Zufic A., Ryšlavá H.:
Seed Protection of Solanum lycopersicum with Pythium oligandrum against Alternaria brassicicola and Verticillium albo-atrum.
Microorganisms 10 (2022) 1348. DOI: 10.3390/microorganisms10071348
Čokrtová K., Mareš V., Křížek T.:
On-capillary fluorescent labeling of saccharides for capillary electrophoresis.
Electrophoresis 44 (2022) 35-43. DOI: 10.1002/elps.202200136
Čokrtová K., Křížek T.:
Liquid crystals purity assay using nonaqueous capillary electrokinetic chromatography.
Electrophoresis 43 (2022) 1638-1646. DOI: 10.1002/elps.202200053
Vymyslický F., Křížek T., Kozlík, P., Kubíčková A., Heřt, J., Bartosińska E.:
Alternative method for canagliflozin oxidation analysis using an electrochemical flow cell - Comparative study. Journal of Pharmaceutical and Biomedical Analysis (2022) 114341. DOI: 10.1016/j.jpba.2021.114341
Krijt J., Sokolová J., Šilhavý J., Mlejnek P., Kubovciak J., Liška F., Malínská H., Hüttl M., Marková I., Křížková M., Stipanuk M. H., Křížek T., Ditroi T., Nagy P., Kožich V., Pravenec M.:
High Cysteine Diet Reduces Insulin Resistance in SHR-CRP Rats.
Physiological Research 70 (2021) 687.
Čokrtová K., Křížek T., Kubíčková A., Coufal P.:
Effect of background electrolyte anions on markers of electroosmotic flow in capillary electrophoresis. Monatshefte für Chemie 152 (2021) 1061-1065. DOI:10.1007/s00706-021-02810-x
Martínková E., Křížek T., Kubíčková A., Coufal P.:
Mobilization of electroosmotic flow markers in capillary zone
electrophoresis. Electrophoresis 42 (2021) 932-938. DOI:10.1002/elps.202000301
Bartosińska E., Kozlík P., Kubíčková A., Heřt J., Fischer J., Křížek T.:
Comparison of static and dynamic mode in the electrochemical oxidation of fesoterodine with the use of experimental design approach. Talanta 226 (2021) 122141. DOI: 10.1016/j.talanta.2021.122141
Křížek T., Molnárová K., Pavlů V., Filounová B., Martínková E.:
Interaction of heparin and tetraarginine in capillary electrophoresis: implication for analytical applications. Electrophoresis 41 (2020) 1826-1831, DOI: 10.1002/elps.202000011
Březina K., Duboué-Dijon E., Palivec V., Jiráček J., Křížek T., Viola C. M., Ganderton T. R., Brzozowski A. M., Jungwirth P.:
Can Arginine Inhibit Insulin Aggregation? A Combined Protein Crystallography, Capillary Electrophoresis and Molecular Simulation Study. Journal of Physical Chemistry B 122 (2018) 10069-10076.
Taraba L., Křížek T., Kozlík P., Hodek O., Coufal P.:
Protonation of polyaniline-coated silica stationary phase affects the retention behavior of neutral hydrophobic solutes in reverse-phased capillary liquid chromatography. Journal of Separation Science (2018) 2865-3008.
Křížek T., Bursová M., Horsley R., Kuchař M., Tůma P., Čabala R., Hložek T.:
Menthol-based hydrophobic deep eutectic solvents: Towards greener and efficient extraction of phytocannabinoids. Journal of Cleaner Production 193 (2018) 391-396.
Hodek O., Křížek T., Coufal P., Ryšlavá H.:
Online screening of alpha-amylase inhibitors by capillary electrophoresis. Analytical and Bioanalytical Chemistry B (2018) 4213-4218.
Duboué-Dijon E., Delcroix P., Martinez-Seara H., Hladílková J., Coufal P., Křížek T., Jungwirth P.:
Binding of divalent cations to insulin: Capillary electrophoresis and molecular simulations. Journal of Physical Chemistry B 122 (2018) 5640-5648.
Hložek T., Křížek T., Tůma P., Bursová M., Coufal P., Čabala R.:
Quantification of paracetamol and 5-oxoproline in serum by capillary electrophoresis: Implication for clinica toxicology. Journal of Pharmaceutical and Biomedical Analysis 145 (2017) 616-620.
Seydlová G., Pohl R., Zborníková E., Ehn M., Šimák O., Panova N., Kolář M., Bogdanová K., Večeřová R., Fišer R., Šanderová H., Vítovská D., Sudzinová P., Pospíšil J., Benada O., Křížek T., Sedlák D., Bartůněk P., Krásný L., Rejman D.:
Lipophosphonoxins II: Design, Synthesis, and Properties of Novel Broad Spectrum Antibacterial Agents. Journal of Medicinal Chemistry 60 (2017) 6098-6118.
Hodek O., Křížek T., Coufal P., Ryšlavá H.:
Design of experiments for amino acid extraction from tobacco leaves and their subsequent determination by capillary zone electrophoresis. Analytical and Bioanalytical Chemistry 409 (2017) 2383-2391.
Taraba L., Křížek T., Kubíčková A., Coufal P.:
Sample pretreatment for the capillary electrophoretic determination of organic acids in chromium(III) plating baths. Journal of Separation Science 38 (2016) 4255-4261.
Martínková E., Křížek T., Coufal P.:
Determination of nitrites and nitrates in drinking water using capillary electrophoresis. Chemical Papers 68 (2014) 1008-1014.
Křížek T., Kubíčková A., Hladílková J., Coufal P., Heyda J., Jungwirth P.:
Electrophoretic mobilities of neutral analytes and electroosmotic flow markers in aqueous solutions of Hofmeister salts. Electrophoresis 35 (2014) 617-624.
Křížek T., Doubnerová V., Ryšlavá H., Coufal P., Bosáková Z.:
Offline and online capillary electrophoresis enzyme assays of beta-N-acetylhexosaminidase. Analytical and Bioanalytical Chemistry, 405 (2013) 2425-2434.
Kubíčková A., Křížek T., Coufal P., Vazdar M., Wernersson E., Heyda J., Jungwirth P.:
Overcharging in biological systems: Reversal of electrophoretic mobility of aqueous Polyaspartate by mutlivalent cations. Physical Review Letters, 18 (2012) Article number 18610.
Křížek T., Breitbach Z. S., Armstrong D. W., Tesařová E., Coufal P.:
Separation of inorganic and small organic anions by CE using phosphonium-based mono- and dicationic reagents. Electrophoresis, 30 (2009) 3955-3963.
INTERNSHIPS
September - December 2008: University of Texas at Arlington, USA (prof. Daniel W. Armstrong)
Application of mono- and dicationic phosphonium additives to background electrolyte for separation of inorganicand small organic anions using capillary electrophoresis.
February 2012: Institut für Pharmazeutische Wissenschaften, Karl-Franzens-Universität, Graz, Austria (prof. Martin Schmid).
Derivatization of cyclofructan 6 and testing the applicability of the derivatives for chiral separations in capillary electrophoresis.
August 2014 - August 2015: Georgia Institute of Technology, School of Chemistry and Biochemistry, USA (prof. Jiří Janata)
Design and bulk preparation of atomic metal clusters.
AWARDS
2011: XV. Meeting of biochemists and molecular biologists - young scientists section - 3rd place.
2012: XV. Shimadzu Prize - 2nd place.
2014: Famelab 2014 - competing in the national final.
2020: Dean's award for young teachers/researchers.
2021: Students' giant salamander award for the best out-of-section subject (Presenting and public speaking)
GRANTS
2006 - 2007: Team member in project with Agilent Technologies: HPLC-chip/MS for analysis of protein digests.
2007 - 2009: Team member in project of Czech Science Foundation GACR 203/07/0329: Study of multidimensional capillary electrophoresis systems for separation of chosen biologically active compounds.
2010 - 2012: Principal investigator in project GAUK 710: Development of efficient and sensitive electromigration methods for determination of substrates and products of glycosidases.
2013 - 2014: Team member in project GACR 13-01440S: Chiral molecular polymers based on derivatized cyclodextrins as a new environment for capillary separation techniques.
2014 - 2015: Fulbright-Masaryk Scholarship.
2017 - 2019: Team member in project TACR TJ01000170: Control and optimization of recycling process of industrial antifreeze mixtures.
2018 - 2019: Team member in project TACR TJ01000451: Industrial research of versatile treatment of seeds and seedlings that protects from fungal, bacterial and other parasites.
2019 - 2021: Team member in project GACR 19-18005Y: Retention mechanism of intact glycopeptides in hydrophilic interaction liquid chromatography.
2020 - 2022: Team member in project TACR TJ4000540: Analysis of specific components of waste and recyclates of coolants.
2022 - 2025: Team member in project AZV NU22-08-00346: Combination of new cannabinoids and advanced formulation methods for treatment of rheumatoid arthritis.
2023 - 2026: Team member in project TAČR STA02023SS060: The most important components of organic detritus for the survival and development of young freshwater pearl mussels.
2025 - 2027: Team member in project Lead Agency/GAČR 25-14463L: Innovative sp3 carbon-based electrode materials and electrochemical reactors for degradation of mixtures of environmental pollutants.
OTHER
2010 - 2012: Supervisor in the "Open Science II" project of the Czech Science Foundation. Topic: Comparison of quality of bottled and tap driking water: Electrophoretic determination of nitrites and nitrates.
2012 - 2014: Supervisor in the "Open Science III" project of the Czech Science Foundation. Topic: Monitoring of benzoic acid content in food using capillary electrophoresis.
- Lector of secondary-school student projects.
- CEEPUS (Central European Exchange Programme for University Studies)
Network RO-0010 Teaching and Learning Bioanalysis – faculty coordinator.