RNDr. Tomáš Křížek, Ph.D.      
  Department of Analytical Chemistry, Door #118
  tel. +420 221 951 229
  e-mail: tomas.krizek@natur.cuni.cz
  consultation hours: flexible, after previous agreement via e-mail.

Analytical chemistry I (a)
Analytical chemistry II (a)
Laboratory exercise in separation techniques
Presenting and public speaking
Chemical calculations
Advances in separation science
Laboratory exercise in basic analytical chemistry
Theoretical basis of analytical chemistry

Famelab - contest for young scientists focused on popularization of science.
Toastmasters - club focused on public speaking and development of communication and leadership skills in English.

Monitoring of interactions of biomolecules using capillary electrophoresis and their analytical applications.
Application of capillary electrophoresis in analysis of biological and industrial materials.
Stability and degradation study of pharmaceutically significant compounds.

Capillary electrophoresis study of interactions of chosen compounds and particles with liposomes - Mgr. Alice Tomniková
Electromigration separations of glycopeptides and glycans - Mgr. Kateřina Čokrtová
Study of stability and degradation of pharmaceutically significant compounds - Mgr. Filip Vymyslický

Diploma theses:
Electrokinetic chromatography in non-aqueous and mixed solvents - Bc. Katarína Hamráková
Analysis and characterisation of liposomes using capillary electrophoresis - Bc. Andrea Orgoníková
Electrochemical oxidation of pharmaceutically important compounds - Bc. Markéta Bolíková
Comparison of detection techniques for saccharide analysis in capillary electrophoresis - Bc. Nikol Vlčková
Study of stability and degradation of pharmaceutically significant compounds - Bc. Kateřina Hrubcová

Bachelor theses:
Electrophoretic determination of proteinogenic amino avids and glutathione in plant material - Eva Durychová
Online derivatization of saccharides in capillary electrophoresis - Vít Mareš
Study of oxidative degradation of pharmaceutically significant compounds - Michaela Tůmová

Determination of proteinogenic amino acids by high-performance separation techniques - RNDr. Ondřej Hodek, Ph.D.
Chromatographic characterization of polyaniline-coated stationary phases - RNDr. Lukáš Taraba, Ph.D.

Diploma theses:
Electrophoretic determination of rivaroxaban - Mgr. Ondřej Petrák
HPLC determination of tryptophan, serotonin and melatonin in plant materials - Mgr. Věra Pavlů
Electrophoretic determination of tantalum in recycled coolants - Mgr. Semen Gogulin
Development of electrophoretic method for determination of phosphates and borates in coolants - Mgr. Iryna Listakhava
Determination of glucosinolates in plant materials - Bc. Veronika Holá
Application of capillary electrophoresis in analysis of saccharide component of glycopeptides - Mgr. Alice Šimonová
Forced degradation study of canagliflozin using HPLC - Mgr. Jitka Máchalová
Determination of low-molecular-mass heparin using affinity capillary electrophoresis - Mgr. Katarína Molnárová
Separation of liquid crystal forming substances using non-aqueous capillary electrokinetic chromatography - Mgr. Kateřina Čokrtová
Utilization of statistical approach in development of HPLC methods - Mgr. Filip Vymyslický
Preparation and characterization of polyaniline-coated stationary phases doped with silver - Mgr. Viktória Pátereková
Indirect determination of heparin using capillary electrophoresis - Mgr. Barbora Filounová
Study of interaction between protamine and heparin and its applicability in capillary electrophoresis - Mgr. Eva Martínková
Study of complexation of vortioxetin with cyclodextrins and its determination by capillary electrophoresis - Mgr. Žofie Počtová
Determination of protamines by capillary zone electrophoresis - Mgr. Michal Malý, Ph.D.
Characterization self-organizing molecules and their application in capillary electrophoresis - RNDr. Ondřej Hodek, Ph.D.
Electrophoretic determination of organic acids in industrial solutions - RNDr. Lukáš Taraba, Ph.D.
Capillary electrophoretic enzyme assay of cellobiase - Mgr. Romana Velvarská

Bachelor theses:
Electrophoretic determination of saccharides in blossom nectar - Bc. Nikola Hurychová
Development of UHPLC method for determination of abirateron acetate and its oxidative degradation - Bc. Nikol Vlčková
Degradation studies of pharmaceutically important compounds - Markéta Bolíková
Development of electrophoretic method for determination of ethanolamines in antifreeze mixtures - Bc. Katarína Hamráková
Monitoring of enzyme activity of myrosinase using capillary electrophoresis in off-line and on-line setup - Bc. Andrea Orgoníková
Extraction of tryptophan and its metabolites from plant material and its separation by micellar electrokinetic chromatography - Bc. Semen Gogulin
Development of electrophoretic method for determination of sulphate in blood plasma - Bc. Lucie Hnízdilová
Study of interaction of heparin and tetraarginine using capillary electrophoresis - Bc. Věra Pavlů
Effect of separation electrolyte ions on baseline stability in capillary electrophoresis - Bc. Tereza Kolečkářová
Characterization of protamine influence on indirect electrophoretic determination of heparin - Ondřej Petrák
Determination of tryptamine and tryptophan in biological materia using capillary electrophoresis - Bc. Alice Šimonová
Development of a UHPLC method for stability study of venetoclax - Bc. Jitka Máchalová
Optimization of electrophoretic determination of protamine and insulin - Bc. Katarína Molnárová
Optimization of background eletrolyte for electrophoretic determination of proteinogennic amino acids - Bc. Adéla Pravdová
Effect of backgroung electrolyte anions on electroosmotic flow markers in capillary electrophoresis - Bc. Kateřina Čokrtová
Mobilization of electroosmotic flow markers in capillary electrophoresis - Mgr. Eva Martínková
Comparison of sensitivity of contactless conductivity and direct UV detection for electrophoretic determination of N-acetylaminosaccharides - Mgr. Michal Malý
Capillary electrophoretic separation and determination of possible products of 4-nitrofenyl-N,N'-diacetyl-beta-D-chitobioside enzyme cleavage - Mgr. Romana Velvarská

ResearcherID L-8349-2017 , ORCID 0000-0002-1623-8844, Scopus Author ID 54936071100, Publons

Vymyslický F., Křížek T., Kozlík, P., Kubíčková A., Heřt, J., Bartosińska E.:
Alternative method for canagliflozin oxidation analysis using an electrochemical flow cell - Comparative study. Journal of Pharmaceutical and Biomedical Analysis (2022) 114341. DOI: 10.1016/j.jpba.2021.114341

Validity of cycloheximide chylomicron flow blocking method for the evaluation of lymphatic transport of drugs. British Journal of Pharmacology 178 (2021) 4663-4674. DOI: 10.1111/bph.15644

Čokrtová K., Křížek T., Kubíčková A., Coufal P.:
Effect of background electrolyte anions on markers of electroosmotic flow in capillary electrophoresis. Monatshefte für Chemie 152 (2021) 1061-1065. DOI:10.1007/s00706-021-02810-x

Martínková E., Křížek T., Kubíčková A., Coufal P.:
Mobilization of electroosmotic flow markers in capillary zone electrophoresis. Electrophoresis 42 (2021) 932-938. DOI:10.1002/elps.202000301

Bartosińska E., Kozlík P., Kubíčková A., Heřt J., Fischer J., Křížek T.:
Comparison of static and dynamic mode in the electrochemical oxidation of fesoterodine with the use of experimental design approach. Talanta 226 (2021) 122141. DOI: 10.1016/j.talanta.2021.122141

Hořínková J., Kozlík P., Křížek T., Michaličková D., Šíma M., Slanař O.:
Post-mortem redistribution of alprazolam in rats. Prague Medical Report 121 (2020) 244-253. DOI: 10.14712/23362936.2020.21

Boleslavská T., Rychecký O., Krov M., Žvátora P., Dammer O., Beránek O., Kozlík P., Křížek T., Hořínková J., Ryšánek P., Roušavá J., Kutinová Canová N., Šíma M., Slanař O., Štěpánek F.:
Bioavailability enhancement and food effect elimination of abiraterone acetate by encapsulation in surfactant-enriched oil marbles. The AAPS Journal 22 (2020) 122. DOI: 10.1208/s12248-020-00505-5

Bělonožníková B., Vaverová K., Vaněk T., Kolařík M., Hýsková V., Vaňková R., Dobrev P., Křížek T., Hodek O., Čokrtová K., Štípek A., Ryšlavá H.:
Novel insights into the efect of Phythium strains on rapeseed metabolism. Microorganisms 8 (2020) 1472. DOI: 10.3390/microorganisms8101472

Křížek T., Müllerová R.:
Sucrose hydrolysis during the preparation of “dandelion honey”. Monatshefte für Chemie 151 (2020) 1231-1234, DOI: 10.1007/s00706-020-02636-z

Kozlík P., Molnárová K., Ječmen T., Křížek T., Goldman R.:
Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC. Journal of Chromatography B 1150 (2020) 122196. DOI: 10.1016/j.jchromb.2020.122196

Křížek T., Kozlík P., Hodek O., Štěpánová E., Nesměrák K.:
Lidocaine adsorption to ethylene-vinyl acetate infusion bags decreases its availability in del Nido cardioplegia solution. Monatshefte für Chemie 151 (2020) 1217-1223, DOI: 10.100/s00706-020-02602-9

Boleslavská T., Světlík S., Žvátora P., Bosák J., Dammer O., Beránek J., Kozlík P., Křížek T., Kutinová Canová N., Šíma M., Slanař O., Štěpánek F.:
Preclinical evaluation of new formulation concepts for abiraterone acetate bioavailability enhancement based on the inhibition of pH-induced precipitation. European Journal of Pharmaceutics and Biopharmaceutics 151 (2020) 81-90. DOI: 10.1016/j.ejpb.2020.04.005

Křížek T., Molnárová K., Pavlů V., Filounová B., Martínková E.:
Interaction of heparin and tetraarginine in capillary electrophoresis: implication for analytical applications. Electrophoresis 41 (2020) 1826-1831, DOI: 10.1002/elps.202000011

Hodek O., Křížek T.:
Determination of short-chain fatty acids in feces by capillary electrophoresis with indirect UV-VIS detection. Analytical Methods 11 (2019) 4575-4579.

Březina K., Duboué-Dijon E., Palivec V., Jiráček J., Křížek T., Viola C. M., Ganderton T. R., Brzozowski A. M., Jungwirth P.:
Can Arginine Inhibit Insulin Aggregation? A Combined Protein Crystallography, Capillary Electrophoresis and Molecular Simulation Study. Journal of Physical Chemistry B 122 (2018) 10069-10076.

Hodek O., Jans U., Yang L. J., Křížek T.:
Chlorpyrifos-methyl oxon hydrolysis and its monitoring by HPLC-MS/MS. Monatshefte für Chemie - Chemical Monthly 149 (2018) 1515-1519.

Taraba L., Křížek T., Kozlík P., Hodek O., Coufal P.:
Protonation of polyaniline-coated silica stationary phase affects the retention behavior of neutral hydrophobic solutes in reverse-phased capillary liquid chromatography. Journal of Separation Science (2018) 2865-3008.

Křížek T., Bursová M., Horsley R., Kuchař M., Tůma P., Čabala R., Hložek T.:
Menthol-based hydrophobic deep eutectic solvents: Towards greener and efficient extraction of phytocannabinoids. Journal of Cleaner Production 193 (2018) 391-396.

Hodek O., Křížek T., Coufal P., Ryšlavá H.:
Online screening of alpha-amylase inhibitors by capillary electrophoresis. Analytical and Bioanalytical Chemistry B (2018) 4213-4218.

Duboué-Dijon E., Delcroix P., Martinez-Seara H., Hladílková J., Coufal P., Křížek T., Jungwirth P.:
Binding of divalent cations to insulin: Capillary electrophoresis and molecular simulations. Journal of Physical Chemistry B 122 (2018) 5640-5648.

Hložek T., Křížek T., Tůma P., Bursová M., Coufal P., Čabala R.:
Quantification of paracetamol and 5-oxoproline in serum by capillary electrophoresis: Implication for clinica toxicology. Journal of Pharmaceutical and Biomedical Analysis 145 (2017) 616-620.

Seydlová G., Pohl R., Zborníková E., Ehn M., Šimák O., Panova N., Kolář M., Bogdanová K., Večeřová R., Fišer R., Šanderová H., Vítovská D., Sudzinová P., Pospíšil J., Benada O., Křížek T., Sedlák D., Bartůněk P., Krásný L., Rejman D.:
Lipophosphonoxins II: Design, Synthesis, and Properties of Novel Broad Spectrum Antibacterial Agents. Journal of Medicinal Chemistry 60 (2017) 6098-6118.

Taraba L., Křížek T.:
Study of polyaniline-coated silica gel as a stationary phase in different modes of capillary liquid chromatography. Monatshefte für Chemie - Chemical Monthly 148 (2017) 1605-1611.

Hodek O., Křížek T., Coufal P., Ryšlavá H.:
Design of experiments for amino acid extraction from tobacco leaves and their subsequent determination by capillary zone electrophoresis. Analytical and Bioanalytical Chemistry 409 (2017) 2383-2391.

Taraba L., Křížek T., Hodek O., Kalíková K., Coufal P.:
Characterization of polyaniline-coated stationary phases by using the linear solvation energy relationships in the hydrophilic interaction liquid chromatography mode using capillary liquid chromatography. Journal of Separation Science 40 (2017) 677-687.

Malý M., Křížek T.:
Determination of protamine and insulin using short-end injection capillary electrophoresis. Chromatographia 79 (2016) 1643-1648.

Taraba L., Křížek T., Kubíčková A., Coufal P.:
Sample pretreatment for the capillary electrophoretic determination of organic acids in chromium(III) plating baths. Journal of Separation Science 38 (2016) 4255-4261.

Gawron E. L., Křížek T., Kowalik M. A., Josowicz M., Janata J.:
Preparation of a carbon-platinum-polyaniline support for atomic metal deposition. Journal of The Electrochemical Society 162 (2015) H432-H427.

Ryšlavá H., Valenta R., Hýsková V., Křížek T., Liberda J., Coufal P.:
Purification and enzymatic characterization of tobacco leaf beta-N-acetylhexosaminidase. Biochimie 107 B (2014) 263-269.

Martínková E., Křížek T., Coufal P.:
Determination of nitrites and nitrates in drinking water using capillary electrophoresis. Chemical Papers 68 (2014) 1008-1014.

Křížek T., Kubíčková A., Hladílková J., Coufal P., Heyda J., Jungwirth P.:
Electrophoretic mobilities of neutral analytes and electroosmotic flow markers in aqueous solutions of Hofmeister salts. Electrophoresis 35 (2014) 617-624.

Křížek T., Doubnerová V., Ryšlavá H., Coufal P., Bosáková Z.:
Offline and online capillary electrophoresis enzyme assays of beta-N-acetylhexosaminidase. Analytical and Bioanalytical Chemistry, 405 (2013) 2425-2434.

Křížek T., Kubíčková A.:
Microscale separation methods for enzyme kinetics assays. Analytical and Bioanalytical Chemistry, 403 (2012) 2185-2195.

Kubíčková A., Křížek T., Coufal P., Vazdar M., Wernersson E., Heyda J., Jungwirth P.:
Overcharging in biological systems: Reversal of electrophoretic mobility of aqueous Polyaspartate by mutlivalent cations. Physical Review Letters, 18 (2012) Article number 18610.

Wernersson E., Heyda J., Kubíčková A., Křížek T., Coufal P., Jungwirth P.:
Counterion condensation in short cationic peptides: Limiting mobilities beyond the Onsager-Fuoss theory. Electrophoresis, 33 (2012) 981-989.

Kubíčková A., Křížek T., Coufal P., Wernersson E., Heyda J., Jungwirth P.:
Guanidinium cations pair with positively charged arginine side chains in water. Journal of Physical Chemistry Letters, 2 (2011) 1387-1389.

Svobodová A., Křížek T., Širc J., Šálek P., Tesařová E., Coufal P., Štulík K.:
Monolithic columns based on a poly(styrene-divinylbenzene-methacrylic acid) copolymer for capillary liquid chromatography of small organic molecules. Journal of Chromatography A, 1218 (2011) 1544-1547.

Wernersson E., Heyda J., Kubíčková A., Křížek T., Coufal P., Jungwirth P.:
Effect of association with sulfate on the electrophoretic mobility of polyarginine and polylysine. Journal of Physical Chemistry B, 114 (2010) 11934-11941.

Křížek T., Coufal P., Tesařová E., Sobotníková J., Bosáková Z.:
Pluronic F-127 as the buffer additive in capillary entangled polymer electrophoresis: Some fundamental aspects. Journal of Separation Science, 33 (2010) 2458-2464.

Franc M., Křížek T., Coufal P., Štulík K.:
Differentiation among various kinds of cheese by identification of casein using HPLC-chip/MS/MS. Journal of Separation Science, 33 (2010) 2515-2519.

Hamplová A., Křížek T., Kubíček V., Bosáková Z., Coufal P.:
Comparison of HPLC and CZE methods for analysis of DOTA-like esters - reaction intermediates in synthesis of magnetic resonance constrast agents. Journal of Separation Science, 33 (2010) 658-663.

Křížek T., Breitbach Z. S., Armstrong D. W., Tesařová E., Coufal P.:
Separation of inorganic and small organic anions by CE using phosphonium-based mono- and dicationic reagents. Electrophoresis, 30 (2009) 3955-3963.

Křížek T., Coufal P., Bosáková Z., Tesařová E., Sobotníková-Suchánková J.:
Fyzikální gely v kapilární gelové elektroforéze a jejich uplatnění v analýze bílkovin. Chemické listy, 103 (2009) 130-135.

September - December 2008: University of Texas at Arlington, USA (prof. Daniel W. Armstrong)
Application of mono- and dicationic phosphonium additives to background electrolyte for separation of inorganicand small organic anions using capillary electrophoresis.

February 2012: Institut für Pharmazeutische Wissenschaften, Karl-Franzens-Universität, Graz, Austria (prof. Martin Schmid).
Derivatization of cyclofructan 6 and testing the applicability of the derivatives for chiral separations in capillary electrophoresis.

August 2014 - August 2015: Georgia Institute of Technology, School of Chemistry and Biochemistry, USA (prof. Jiří Janata)
Design and bulk preparation of atomic metal clusters.

2011: XV. Meeting of biochemists and molecular biologists - young scientists section - 3rd place.

2012: XV. Shimadzu Prize - 2nd place.

2014: Famelab 2014 - competing in the national final.

2020: Dean's award for young teachers/researchers.

2021: Students' giant salamander award for the best out-of-section subject (Presenting and public speaking)

2006 - 2007: Team member in project with Agilent Technologies: HPLC-chip/MS for analysis of protein digests.

2007 - 2009: Team member in project of Czech Science Foundation GACR 203/07/0329: Study of multidimensional capillary electrophoresis systems for separation of chosen biologically active compounds.

2010 - 2012: Principal investigator in project GAUK 710: Development of efficient and sensitive electromigration methods for determination of substrates and products of glycosidases.

2013 - 2014: Team member in project GACR 13-01440S: Chiral molecular polymers based on derivatized cyclodextrins as a new environment for capillary separation techniques.

2014 - 2015: Fulbright-Masaryk Scholarship.

2017 - 2019: Team member in project TACR TJ01000170: Control and optimization of recycling process of industrial antifreeze mixtures.

2018 - 2019: Team member in project TACR TJ01000451: Industrial research of versatile treatment of seeds and seedlings that protects from fungal, bacterial and other parasites.

2019 - present: Team member in project GACR 19-18005Y: Retention mechanism of intact glycopeptides in hydrophilic interaction liquid chromatography.

2020 - present: Team member in project TACR TJ4000540: Analysis of specific components of waste and recyclates of coolants.

2010 - 2012: Supervisor in the "Open Science II" project of the Czech Science Foundation. Topic: Comparison of quality of bottled and tap driking water: Electrophoretic determination of nitrites and nitrates.

2012 - 2014: Supervisor in the "Open Science III" project of the Czech Science Foundation. Topic: Monitoring of benzoic acid content in food using capillary electrophoresis.

- Lector of secondary-school student projects.