RNDr. Tomáš Křížek, Ph.D.
Department of Analytical Chemistry, Door #118
tel. +420 221 951 229
e-mail: tomas.krizek@natur.cuni.cz
consultation hours: flexible, after previous agreement via e-mail.
TEACHING
Advances in Separation Science
Analytical Chemistry I (a)
Analytical Chemistry II (a)
Electromigration methods
Laboratory Course on Separation Methods
Chemical Calculations
Presenting and Public Speaking
MIGHT BE INTERESTING FOR YOU
Toastmasters - club focused on public speaking and development of communication and leadership skills in English.
RESEARCH INTERESTS
Capillary electrophoresis in investigation of intermolecular interactions, enzyme reactions, and controlled drug release studies.
Application of separation techniques in combination with electrochemistry for stability and forced degradation studies of pharmaceutical substances.
Applications of capillary electrophoresis for biochemical, biological, and medical research.
THESES CURRENTLY IN PROGRESS
Dissertations:
Capillary electrophoresis study of interactions of chosen compounds and particles with liposomes - Mgr. Alice Tomniková
Electromigration separations of glycopeptides and glycans - Mgr. Kateřina Čokrtová
Study of stability and degradation of pharmaceutically significant compounds - Mgr. Filip Vymyslický
Diploma theses:
Electrokinetic chromatography in non-aqueous and mixed solvents - Bc. Katarína Hamráková
Analysis and characterisation of liposomes using capillary electrophoresis - Bc. Andrea Orgoníková
Electrochemical oxidation of pharmaceutically important compounds - Bc. Markéta Bolíková
Comparison of detection techniques for saccharide analysis in capillary electrophoresis - Bc. Nikol Vlčková
Study of stability and degradation of pharmaceutically significant compounds - Bc. Kateřina Hrubcová
Bachelor theses:
Electrophoretic determination of proteinogenic amino avids and glutathione in plant material - Eva Durychová
Online derivatization of saccharides in capillary electrophoresis - Vít Mareš
Study of oxidative degradation of pharmaceutically significant compounds - Michaela Tůmová
SUCCESFULLY DEFENDED THESES
Dissertations:
Determination of proteinogenic amino acids by high-performance separation techniques - RNDr. Ondřej Hodek, Ph.D.
Chromatographic characterization of polyaniline-coated stationary phases - RNDr. Lukáš Taraba, Ph.D.
Diploma theses:
Electrophoretic determination of rivaroxaban - Mgr. Ondřej Petrák
HPLC determination of tryptophan, serotonin and melatonin in plant materials - Mgr. Věra Pavlů
Electrophoretic determination of tantalum in recycled coolants - Mgr. Semen Gogulin
Development of electrophoretic method for determination of phosphates and borates in coolants - Mgr. Iryna Listakhava
Determination of glucosinolates in plant materials - Bc. Veronika Holá
Application of capillary electrophoresis in analysis of saccharide component of glycopeptides - Mgr. Alice Šimonová
Forced degradation study of canagliflozin using HPLC - Mgr. Jitka Máchalová
Determination of low-molecular-mass heparin using affinity capillary electrophoresis - Mgr. Katarína Molnárová
Separation of liquid crystal forming substances using non-aqueous capillary electrokinetic chromatography - Mgr. Kateřina Čokrtová
Utilization of statistical approach in development of HPLC methods - Mgr. Filip Vymyslický
Preparation and characterization of polyaniline-coated stationary phases doped with silver - Mgr. Viktória Pátereková
Indirect determination of heparin using capillary electrophoresis - Mgr. Barbora Filounová
Study of interaction between protamine and heparin and its applicability in capillary electrophoresis - Mgr. Eva Martínková
Study of complexation of vortioxetin with cyclodextrins and its determination by capillary electrophoresis - Mgr. Žofie Počtová
Determination of protamines by capillary zone electrophoresis - Mgr. Michal Malý, Ph.D.
Characterization self-organizing molecules and their application in capillary electrophoresis - RNDr. Ondřej Hodek, Ph.D.
Electrophoretic determination of organic acids in industrial solutions - RNDr. Lukáš Taraba, Ph.D.
Capillary electrophoretic enzyme assay of cellobiase - Mgr. Romana Velvarská
Bachelor theses:
Electrophoretic determination of saccharides in blossom nectar - Bc. Nikola Hurychová
Development of UHPLC method for determination of abirateron acetate and its oxidative degradation - Bc. Nikol Vlčková
Degradation studies of pharmaceutically important compounds - Markéta Bolíková
Development of electrophoretic method for determination of ethanolamines in antifreeze mixtures - Bc. Katarína Hamráková
Monitoring of enzyme activity of myrosinase using capillary electrophoresis in off-line and on-line setup - Bc. Andrea Orgoníková
Extraction of tryptophan and its metabolites from plant material and its separation by micellar electrokinetic chromatography - Bc. Semen Gogulin
Development of electrophoretic method for determination of sulphate in blood plasma - Bc. Lucie Hnízdilová
Study of interaction of heparin and tetraarginine using capillary electrophoresis - Bc. Věra Pavlů
Effect of separation electrolyte ions on baseline stability in capillary electrophoresis - Bc. Tereza Kolečkářová
Characterization of protamine influence on indirect electrophoretic determination of heparin - Ondřej Petrák
Determination of tryptamine and tryptophan in biological materia using capillary electrophoresis - Bc. Alice Šimonová
Development of a UHPLC method for stability study of venetoclax - Bc. Jitka Máchalová
Optimization of electrophoretic determination of protamine and insulin - Bc. Katarína Molnárová
Optimization of background eletrolyte for electrophoretic determination of proteinogennic amino acids - Bc. Adéla Pravdová
Effect of backgroung electrolyte anions on electroosmotic flow markers in capillary electrophoresis - Bc. Kateřina Čokrtová
Mobilization of electroosmotic flow markers in capillary electrophoresis - Mgr. Eva Martínková
Comparison of sensitivity of contactless conductivity and direct UV detection for electrophoretic determination of N-acetylaminosaccharides - Mgr. Michal Malý
Capillary electrophoretic separation and determination of possible products of 4-nitrofenyl-N,N'-diacetyl-beta-D-chitobioside enzyme cleavage - Mgr. Romana Velvarská
PAPERS
ResearcherID L-8349-2017 , ORCID 0000-0002-1623-8844, Scopus Author ID 54936071100, Publons
Šimonová A., Balouch M., Štěpánek F., Křížek T.:
Investigating drug-liposome interactions using liposomal electrokinetic chromatography.
Analytical and Bioanalytical Chemistry 417 (2025) 2029-2038. DOI: 10.1007/s00216-025-05783-6
Brzobohatá H., Dugic M., Mojr V., Sahattsapan N., Kósiová I., Křížek T., Dolejšová T., Lišková P., Cwiklik L., Rejman D., Fišer R., Mikušová G:
LEGO-Lipophosphonoxin membrane activity is enhanced by presence of phosphatidylethanolamine but hindered by outer membrane.
Scientific Reports 15 (2025) 1206. DOI: 10.1038/s41598-024-83205-w
Dolejšová T., Lišková P., Sahattsapan N., Mojr V., Pohl R., Brzobohatá H., Dugic M., Křížek T., Cwiklik L., Mikušová G., Rejman D., Fišer R:
Naphthylated LEGO-lipophosphonoxin antibiotics used as a fluorescent tool for the observation of target membrane perturbations preceding its disruption. JMethods and Applications in Fluorescence (2025) 015001. DOI: 10.1088/2050-6120/ad8abf
Frejková M., Behalová K., Rubanová D., De Sanctis J. B., Kubala L., Chytil P., Šimonová A., Křížek T., Randárová E., Gunár K., Etrych T.:
Polymer nanotherapeutics with the controlled release of acetylsalicylic acid and its derivatives inhibiting cyclooxygenase isoforms and reducing the production of pro-inflammatory mediators.
International Journal of Pharmaceutics 665 (2024) 124742. DOI: 10.1016/j.ijpharm.2024.124742
Šimonová A., Píplová R., Balouch M., Štěpánek F., Křížek T.:
A comprehensive study on capillary surface modifications for electrophoretic separations of liposomes.
Monatshefte für Chemie - Chemical Monthly 155 (2024) 835-844. DOI: 10.1007/s00706-024-03235-y
Merdita S., Šíma M., Dvořák J., Matějů M., Richter I., Kozlík P., Křížek T., Královičová J., Bosák J., Petruželka L., Slanař O.:
Evaluation of adherence to abiraterone therapy in prostate cancer patients based on a population pharmacokinetic model.
British Journal of Clinical Pharmacology 90 (2024) 2652-2662. DOI: 10.1111/bcp.16155
Bosák J., Šíma M., Krejčí T., Obadalová I., Šmardová J., Kozlík P., Křížek T., Beránek J., Hauser T., Slanař O.:
Development of immediate-release formulation with reliable absorption of rivaroxaban in various meal regimes. CTS-Clinical and Traslational Science 17 (2024) E13820. DOI: 10.1111/cts.13820
Molnárová K., Křížek T., Kozlík P.:
The potential of polyaniline-coated stationary phase in hydrophilic interaction liquid chromatography-based solid-phase extraction for glycopeptide enrichment.
Journal of Chromatography B 1237 (2024) 124099. DOI: 10.1016/j.jchromb.2024.124099
Pozniak J, Ryšánek P., Smrčka D., Kozlík P., Křížek T., Šmardová J., Nováková A., Das D., Bobek D., Arora M., Hofmann J., Doušová T., Šíma M., Slanař O.:
Ivacaftor pharmacokinetics and lymphatic transport after enteral administration in rats.
Frontiers in Pharmacology 15 (2024) 1331637. DOI: 10.3389/fphar.2024.1331637
Hýsková V., Jákl M., Dytrtová J. J., Zeljkovic S. C., Vrobel O., Bělonožníková K., Kavan D., Křížek T., Šimonová A., Vašková M., Kovac I., Zufic A. R., Ryšlavá H.:
Antifungal triazoles affect key non-target metabolic pathways in Solanum lycopersicum L. plants
Ecotoxicology and Environmental Safety 268 (2023) 115729. DOI: 10.1016/j.ecoenv.2023.115729
Dushkov A., Vosáhlová Z., Tzintzarov A., Kalíková K., Křížek T., Ugrinova I:
Analysis of the Ibotenic Acid, Muscimol, and Ergosterol Content of an Amanita Muscaria Hydroalcoholic Extract with an Evaluation of Its Cytotoxic Effect against a Panel of Lung Cell Lines In Vitro.
Molecules 28 (2023) 6824. DOI: 10.3390/molecules28196824
Hýsková V., Jákl M., Dytrtová J.J., Zeljkovic S. C., Vrobel O., Bělonožníková K., Kavan D., Křížek T., Šimonová A., Vašková M., Kovac I., Zufic A. R., Ryšlavá H.:
Triazoles as a Potential Threat to the Nutritional Quality of Tomato Fruits.
Metabolites 13 (2023) 988. DOI: 10.3390/metabo13090988
Libánská A., Špringer T., Peštová L, Kotalík K., Konefal R., Šimonová A., Křížek T., Homola J., Randárová E., Etrych T.:
Using surface plasmon resonance, capillary electrophoresis and diffusion-ordered NMR spectroscopy to study drug release kinetics.
Communications Chemistry 6 (2023) 180. DOI: 10.1038/s42004-023-00992-5
Vlčková N., Šimonová A., Duriš M., Čokrtová K., Almquist S, Křížek T.:
Detection techniques for carbohydrates in capillary electrophoresis - a comparative study.
Monatshefte für Chemie - Chemical Monthly 154 (2023) 967-975. DOI: 10.1007/s00706-023-03109-9
Bělonožníková K., Černý M., Hýsková V., Synková H., Valcke R., Hodek O., Křížek T., Kavan D., Vaňková R., Dobrev P., Haisel D., Ryšlavá H:
Casein as protein and hydrolysate: Biostimulant or nitrogen source for Nicotiana tabacum plants grown in vitro?
Physiologia Plantarum 175 (2023) e13973. DOI: 10.1111/ppl.13973
Šalamúnová P., Krejčí T., Ryšánek P., Salon I., Kroupová J., Hubatová-Vacková A., Petřík J., Grus T., Lukáč P., Kozlík P., Křížek T., Dammer O., Beránek J., Šíma M., Slanař O., Štěpánek F.:
Serum and lymph pharmacokinetics of nilotinib delivered by yeast glucan particles per os.
International Journal of Pharmaceutics 634 (2023) 122627. DOI: 10.1016/j.ijpharm.2023.122627
Jelínek P., Roušarová J., Ryšánek P., Ježková M., Havlujová T., Pozniak J., Kozlík P., Křížek T., Kučera T., Šíma M., Slanař O., Šoóš M.:
Application of Oil-in-Water Cannabidiol Emulsion for the Treatment of Rheumatoid Arthritis.
Cannabis and Cannabinoid Research 9 (2022) 147-159. DOI: 10.1089/can.2022.0176
Kožich V., Schwahn B. C., Sokolová J., Křížková M., Ditroi T., Krijt J., Khalil Y., Křížek T., Vaculíková-Fantlová T., Stibůrková B., Mills P., Clayton P., Barvíková K., Blessing H., Sykut-Cegielska J., Dionisi-Vici C., Gasperini S., García-Cazorla A., Haack T. B., Honzík T., Ješina P., Kuster A., Laugwitz L., Martinelli D., Porta F., Santer R., Schwart G., Nagy P.:
Human ultrarare genetic disorders of sulfur metabolism demonstrate redundancies in H2S homeostasis.
Redox Biology 58 (2022) 102517. DOI: 10.1016/j.redox.2022.102517
Petřík J., Rychecký O, Krejčí T., Becherová L., Trunov D., Prachár M., Navrátil O., Žvátora P., Krejčík L., Dammer O., Beránek J., Kozlík P., Křížek T., Šoóš M., Heřt J., Bissola S., Berto S., Štěpánek F.:
Pharmaceutical Product Characterization and Manufacturability of Surfactant-Enriched Oil Marbles with Abiraterone Acetate.
AAPS Pharmscitech 23 (2022) 274. DOI: 10.1208/s12249-022-02430-6
Tomnikova A., Kozlík P., Křížek T.:
Monosaccharide profiling of glycoproteins by capillary electrophoresis with contactless conductivity detection.
Electrophoresis 43 (2022) 1963-1970. DOI: 10.1002/elps.202200033
Tomnikova A., Orgoníková A., Křížek T.:
Liposomes: preparation and characterization with a special focus on the application of capillary electrophoresis.
Monatshefte für Chemie - Chemical Monthly 153 (2022) 687-695. DOI: 10.1007/s00706-022-02966-0
Do Pham D. D., Mojr V., Helusová M., Mikušová G., Pohl R., Dávidová E., Šanderová H., Vítovská D., Bogdanová K., Večeřová R., Sedláková M. H., Fišer R., Sudzinová P., Galandáková A., Kolár M., Krásný L., Rejman D.:
LEGO-Lipophosphonoxins: A Novel Approach in Designing Membrane Targeting Antimicrobials.
Journal of Medicinal Chemistry 65 (2022) 10045-10078. DOI: 10.1021/acs.jmedchem.2c00684
Musilová A., Vojta J., Křížek T., Kubíčková A., Coufal P.:
Capillary monolithic columns based on poly(styrene-divinylbenzene-methacrylic acid) copolymer for liquid chromatography and electrochromatography.
Monatshefte für Chemie - Chemical Monthly 153 (2022) 759-765. DOI: 10.1007/s00706-022-02944-6
Bělonožníková K., Hýsková V., Vašková M., Křížek T., Čokrtová K., Vanět T., Halířová L., Chudý M., Zufic A., Ryšlavá H.:
Seed Protection of Solanum lycopersicum with Pythium oligandrum against Alternaria brassicicola and Verticillium albo-atrum.
Microorganisms 10 (2022) 1348. DOI: 10.3390/microorganisms10071348
Čokrtová K., Mareš V., Křížek T.:
On-capillary fluorescent labeling of saccharides for capillary electrophoresis.
Electrophoresis 44 (2022) 35-43. DOI: 10.1002/elps.202200136
Molnárová K., Čokrtová K., Tomnikova A., Křížek T., Kozlík P.:
Liquid chromatography and capillary electrophoresis in glycomic and glycoproteomic analysis.
Monatshefte für Chemie - Chemical Monthly 153 (2022) 659-686. DOI: 10.1007/s00706-022-02938-4
Čokrtová K., Křížek T.:
Liquid crystals purity assay using nonaqueous capillary electrokinetic chromatography.
Electrophoresis 43 (2022) 1638-1646. DOI: 10.1002/elps.202200053
Kozlík P., Molnárová K., Ječmen T., Křížek T., Bosáková Z.:
Prediction of Intact N-Glycopeptide Retention Time Windows in Hydrophilic Interaction Liquid Chromatography.
Molecules 27 (2022) 3723. DOI: 10.3390/molecules27123723
Hrinová E., Skořepová E., Čerňa I., Královicová J., Kozlík P., Křížek T., Roušarová J., Ryšánek P., Šíma M., Slanař O., Šoóš M.:
Explaining dissolution properties of rivaroxaban cocrystals.
International Journal of Pharmaceutics 622 (2022) 121854. DOI: 10.1016/j.ijpharm.2022.121854
Královicová J., Bartůněk A., Hofmann J., Ryšánek P., Šíma M., Slanař O.:
Pharmacokinetic Variability in Pre-Clinical Studies: Sample Study with Abiraterone in Rats and Implications for Short-Term Comparative Pharmacokinetic Study Designs.
Pharmaceutics 14 (2022) 643. DOI: 10.3390/pharmaceutics14030643
Roušarová J., Šíma M., Kozlík P., Křížek T., Slanař O.:
Changes in Rosuvastatin Pharmacokinetics During Postnatal Ontogenesis in Rats.
Journal of Pharmacy and Pharmaceutical Sciences 25 (2022) 1-8.
Vymyslický F., Křížek T., Kozlík, P., Kubíčková A., Heřt, J., Bartosińska E.:
Alternative method for canagliflozin oxidation analysis using an electrochemical flow cell - Comparative study. Journal of Pharmaceutical and Biomedical Analysis (2022) 114341. DOI: 10.1016/j.jpba.2021.114341
Krijt J., Sokolová J., Šilhavý J., Mlejnek P., Kubovciak J., Liška F., Malínská H., Hüttl M., Marková I., Křížková M., Stipanuk M. H., Křížek T., Ditroi T., Nagy P., Kožich V., Pravenec M.:
High Cysteine Diet Reduces Insulin Resistance in SHR-CRP Rats.
Physiological Research 70 (2021) 687.
Ryšánek P., Grus T., Lukáš P., Kozlík P., Křížek T., Pozniak J., Roušarová J., Kralovicová J., Canová N. K., Boleslavská T., Bosák J., Štěpánek F., Šíma M., Slanař O.:
Validity of cycloheximide chylomicron flow blocking method for the evaluation of lymphatic transport of drugs. British Journal of Pharmacology 178 (2021) 4663-4674. DOI: 10.1111/bph.15644
Čokrtová K., Křížek T., Kubíčková A., Coufal P.:
Effect of background electrolyte anions on markers of electroosmotic flow in capillary electrophoresis. Monatshefte für Chemie 152 (2021) 1061-1065. DOI:10.1007/s00706-021-02810-x
Martínková E., Křížek T., Kubíčková A., Coufal P.:
Mobilization of electroosmotic flow markers in capillary zone electrophoresis. Electrophoresis 42 (2021) 932-938. DOI:10.1002/elps.202000301
Bartosińska E., Kozlík P., Kubíčková A., Heřt J., Fischer J., Křížek T.:
Comparison of static and dynamic mode in the electrochemical oxidation of fesoterodine with the use of experimental design approach. Talanta 226 (2021) 122141. DOI: 10.1016/j.talanta.2021.122141
Hořínková J., Kozlík P., Křížek T., Michaličková D., Šíma M., Slanař O.:
Post-mortem redistribution of alprazolam in rats. Prague Medical Report 121 (2020) 244-253. DOI: 10.14712/23362936.2020.21
Boleslavská T., Rychecký O., Krov M., Žvátora P., Dammer O., Beránek O., Kozlík P., Křížek T., Hořínková J., Ryšánek P., Roušavá J., Kutinová Canová N., Šíma M., Slanař O., Štěpánek F.:
Bioavailability enhancement and food effect elimination of abiraterone acetate by encapsulation in surfactant-enriched oil marbles. The AAPS Journal 22 (2020) 122. DOI: 10.1208/s12248-020-00505-5
Bělonožníková B., Vaverová K., Vaněk T., Kolařík M., Hýsková V., Vaňková R., Dobrev P., Křížek T., Hodek O., Čokrtová K., Štípek A., Ryšlavá H.:
Novel insights into the efect of Phythium strains on rapeseed metabolism. Microorganisms 8 (2020) 1472. DOI: 10.3390/microorganisms8101472
Křížek T., Müllerová R.:
Sucrose hydrolysis during the preparation of “dandelion honey”. Monatshefte für Chemie 151 (2020) 1231-1234, DOI: 10.1007/s00706-020-02636-z
Kozlík P., Molnárová K., Ječmen T., Křížek T., Goldman R.:
Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC. Journal of Chromatography B 1150 (2020) 122196. DOI: 10.1016/j.jchromb.2020.122196
Křížek T., Kozlík P., Hodek O., Štěpánová E., Nesměrák K.:
Lidocaine adsorption to ethylene-vinyl acetate infusion bags decreases its availability in del Nido cardioplegia solution. Monatshefte für Chemie 151 (2020) 1217-1223, DOI: 10.100/s00706-020-02602-9
Boleslavská T., Světlík S., Žvátora P., Bosák J., Dammer O., Beránek J., Kozlík P., Křížek T., Kutinová Canová N., Šíma M., Slanař O., Štěpánek F.:
Preclinical evaluation of new formulation concepts for abiraterone acetate bioavailability enhancement based on the inhibition of pH-induced precipitation. European Journal of Pharmaceutics and Biopharmaceutics 151 (2020) 81-90. DOI: 10.1016/j.ejpb.2020.04.005
Křížek T., Molnárová K., Pavlů V., Filounová B., Martínková E.:
Interaction of heparin and tetraarginine in capillary electrophoresis: implication for analytical applications. Electrophoresis 41 (2020) 1826-1831, DOI: 10.1002/elps.202000011
Hodek O., Křížek T.:
Determination of short-chain fatty acids in feces by capillary electrophoresis with indirect UV-VIS detection. Analytical Methods 11 (2019) 4575-4579.
Březina K., Duboué-Dijon E., Palivec V., Jiráček J., Křížek T., Viola C. M., Ganderton T. R., Brzozowski A. M., Jungwirth P.:
Can Arginine Inhibit Insulin Aggregation? A Combined Protein Crystallography, Capillary Electrophoresis and Molecular Simulation Study. Journal of Physical Chemistry B 122 (2018) 10069-10076.
Hodek O., Jans U., Yang L. J., Křížek T.:
Chlorpyrifos-methyl oxon hydrolysis and its monitoring by HPLC-MS/MS. Monatshefte für Chemie - Chemical Monthly 149 (2018) 1515-1519.
Taraba L., Křížek T., Kozlík P., Hodek O., Coufal P.:
Protonation of polyaniline-coated silica stationary phase affects the retention behavior of neutral hydrophobic solutes in reverse-phased capillary liquid chromatography. Journal of Separation Science (2018) 2865-3008.
Křížek T., Bursová M., Horsley R., Kuchař M., Tůma P., Čabala R., Hložek T.:
Menthol-based hydrophobic deep eutectic solvents: Towards greener and efficient extraction of phytocannabinoids. Journal of Cleaner Production 193 (2018) 391-396.
Hodek O., Křížek T., Coufal P., Ryšlavá H.:
Online screening of alpha-amylase inhibitors by capillary electrophoresis. Analytical and Bioanalytical Chemistry B (2018) 4213-4218.
Duboué-Dijon E., Delcroix P., Martinez-Seara H., Hladílková J., Coufal P., Křížek T., Jungwirth P.:
Binding of divalent cations to insulin: Capillary electrophoresis and molecular simulations. Journal of Physical Chemistry B 122 (2018) 5640-5648.
Hložek T., Křížek T., Tůma P., Bursová M., Coufal P., Čabala R.:
Quantification of paracetamol and 5-oxoproline in serum by capillary electrophoresis: Implication for clinica toxicology. Journal of Pharmaceutical and Biomedical Analysis 145 (2017) 616-620.
Seydlová G., Pohl R., Zborníková E., Ehn M., Šimák O., Panova N., Kolář M., Bogdanová K., Večeřová R., Fišer R., Šanderová H., Vítovská D., Sudzinová P., Pospíšil J., Benada O., Křížek T., Sedlák D., Bartůněk P., Krásný L., Rejman D.:
Lipophosphonoxins II: Design, Synthesis, and Properties of Novel Broad Spectrum Antibacterial Agents. Journal of Medicinal Chemistry 60 (2017) 6098-6118.
Taraba L., Křížek T.:
Study of polyaniline-coated silica gel as a stationary phase in different modes of capillary liquid chromatography. Monatshefte für Chemie - Chemical Monthly 148 (2017) 1605-1611.
Hodek O., Křížek T., Coufal P., Ryšlavá H.:
Design of experiments for amino acid extraction from tobacco leaves and their subsequent determination by capillary zone electrophoresis. Analytical and Bioanalytical Chemistry 409 (2017) 2383-2391.
Taraba L., Křížek T., Hodek O., Kalíková K., Coufal P.:
Characterization of polyaniline-coated stationary phases by using the linear solvation energy relationships in the hydrophilic interaction liquid chromatography mode using capillary liquid chromatography. Journal of Separation Science 40 (2017) 677-687.
Malý M., Křížek T.:
Determination of protamine and insulin using short-end injection capillary electrophoresis. Chromatographia 79 (2016) 1643-1648.
Taraba L., Křížek T., Kubíčková A., Coufal P.:
Sample pretreatment for the capillary electrophoretic determination of organic acids in chromium(III) plating baths. Journal of Separation Science 38 (2016) 4255-4261.
Gawron E. L., Křížek T., Kowalik M. A., Josowicz M., Janata J.:
Preparation of a carbon-platinum-polyaniline support for atomic metal deposition. Journal of The Electrochemical Society 162 (2015) H432-H427.
Ryšlavá H., Valenta R., Hýsková V., Křížek T., Liberda J., Coufal P.:
Purification and enzymatic characterization of tobacco leaf beta-N-acetylhexosaminidase. Biochimie 107 B (2014) 263-269.
Martínková E., Křížek T., Coufal P.:
Determination of nitrites and nitrates in drinking water using capillary electrophoresis. Chemical Papers 68 (2014) 1008-1014.
Křížek T., Kubíčková A., Hladílková J., Coufal P., Heyda J., Jungwirth P.:
Electrophoretic mobilities of neutral analytes and electroosmotic flow markers in aqueous solutions of Hofmeister salts. Electrophoresis 35 (2014) 617-624.
Křížek T., Doubnerová V., Ryšlavá H., Coufal P., Bosáková Z.:
Offline and online capillary electrophoresis enzyme assays of beta-N-acetylhexosaminidase. Analytical and Bioanalytical Chemistry, 405 (2013) 2425-2434.
Křížek T., Kubíčková A.:
Microscale separation methods for enzyme kinetics assays. Analytical and Bioanalytical Chemistry, 403 (2012) 2185-2195.
Kubíčková A., Křížek T., Coufal P., Vazdar M., Wernersson E., Heyda J., Jungwirth P.:
Overcharging in biological systems: Reversal of electrophoretic mobility of aqueous Polyaspartate by mutlivalent cations. Physical Review Letters, 18 (2012) Article number 18610.
Wernersson E., Heyda J., Kubíčková A., Křížek T., Coufal P., Jungwirth P.:
Counterion condensation in short cationic peptides: Limiting mobilities beyond the Onsager-Fuoss theory. Electrophoresis, 33 (2012) 981-989.
Kubíčková A., Křížek T., Coufal P., Wernersson E., Heyda J., Jungwirth P.:
Guanidinium cations pair with positively charged arginine side chains in water. Journal of Physical Chemistry Letters, 2 (2011) 1387-1389.
Svobodová A., Křížek T., Širc J., Šálek P., Tesařová E., Coufal P., Štulík K.:
Monolithic columns based on a poly(styrene-divinylbenzene-methacrylic acid) copolymer for capillary liquid chromatography of small organic molecules. Journal of Chromatography A, 1218 (2011) 1544-1547.
Wernersson E., Heyda J., Kubíčková A., Křížek T., Coufal P., Jungwirth P.:
Effect of association with sulfate on the electrophoretic mobility of polyarginine and polylysine. Journal of Physical Chemistry B, 114 (2010) 11934-11941.
Křížek T., Coufal P., Tesařová E., Sobotníková J., Bosáková Z.:
Pluronic F-127 as the buffer additive in capillary entangled polymer electrophoresis: Some fundamental aspects. Journal of Separation Science, 33 (2010) 2458-2464.
Franc M., Křížek T., Coufal P., Štulík K.:
Differentiation among various kinds of cheese by identification of casein using HPLC-chip/MS/MS. Journal of Separation Science, 33 (2010) 2515-2519.
Hamplová A., Křížek T., Kubíček V., Bosáková Z., Coufal P.:
Comparison of HPLC and CZE methods for analysis of DOTA-like esters - reaction intermediates in synthesis of magnetic resonance constrast agents. Journal of Separation Science, 33 (2010) 658-663.
Křížek T., Breitbach Z. S., Armstrong D. W., Tesařová E., Coufal P.:
Separation of inorganic and small organic anions by CE using phosphonium-based mono- and dicationic reagents. Electrophoresis, 30 (2009) 3955-3963.
Křížek T., Coufal P., Bosáková Z., Tesařová E., Sobotníková-Suchánková J.:
Fyzikální gely v kapilární gelové elektroforéze a jejich uplatnění v analýze bílkovin. Chemické listy, 103 (2009) 130-135.
INTERNSHIPS
September - December 2008: University of Texas at Arlington, USA (prof. Daniel W. Armstrong)
Application of mono- and dicationic phosphonium additives to background electrolyte for separation of inorganicand small organic anions using capillary electrophoresis.
February 2012: Institut für Pharmazeutische Wissenschaften, Karl-Franzens-Universität, Graz, Austria (prof. Martin Schmid).
Derivatization of cyclofructan 6 and testing the applicability of the derivatives for chiral separations in capillary electrophoresis.
August 2014 - August 2015: Georgia Institute of Technology, School of Chemistry and Biochemistry, USA (prof. Jiří Janata)
Design and bulk preparation of atomic metal clusters.
AWARDS
2011: XV. Meeting of biochemists and molecular biologists - young scientists section - 3rd place.
2012: XV. Shimadzu Prize - 2nd place.
2014: Famelab 2014 - competing in the national final.
2020: Dean's award for young teachers/researchers.
2021: Students' giant salamander award for the best out-of-section subject (Presenting and public speaking)
GRANTS
2006 - 2007: Team member in project with Agilent Technologies: HPLC-chip/MS for analysis of protein digests.
2007 - 2009: Team member in project of Czech Science Foundation GACR 203/07/0329: Study of multidimensional capillary electrophoresis systems for separation of chosen biologically active compounds.
2010 - 2012: Principal investigator in project GAUK 710: Development of efficient and sensitive electromigration methods for determination of substrates and products of glycosidases.
2013 - 2014: Team member in project GACR 13-01440S: Chiral molecular polymers based on derivatized cyclodextrins as a new environment for capillary separation techniques.
2014 - 2015: Fulbright-Masaryk Scholarship.
2017 - 2019: Team member in project TACR TJ01000170: Control and optimization of recycling process of industrial antifreeze mixtures.
2018 - 2019: Team member in project TACR TJ01000451: Industrial research of versatile treatment of seeds and seedlings that protects from fungal, bacterial and other parasites.
2019 - 2021: Team member in project GACR 19-18005Y: Retention mechanism of intact glycopeptides in hydrophilic interaction liquid chromatography.
2020 - 2022: Team member in project TACR TJ4000540: Analysis of specific components of waste and recyclates of coolants.
2022 - 2025: Team member in project AZV NU22-08-00346: Combination of new cannabinoids and advanced formulation methods for treatment of rheumatoid arthritis.
2023 - 2026: Team member in project TAČR STA02023SS060: The most important components of organic detritus for the survival and development of young freshwater pearl mussels.
2025 - 2027: Team member in project Lead Agency/GAČR 25-14463L: Innovative sp3 carbon-based electrode materials and electrochemical reactors for degradation of mixtures of environmental pollutants.
OTHER
2010 - 2012: Supervisor in the "Open Science II" project of the Czech Science Foundation. Topic: Comparison of quality of bottled and tap driking water: Electrophoretic determination of nitrites and nitrates.
2012 - 2014: Supervisor in the "Open Science III" project of the Czech Science Foundation. Topic: Monitoring of benzoic acid content in food using capillary electrophoresis.
- Lector of secondary-school student projects.
- CEEPUS (Central European Exchange Programme for University Studies)
Network RO-0010 Teaching and Learning Bioanalysis – faculty coordinator.