Avian parasites study group
Jana Rádrová Thesis: Bloodsucking diptera as hosts and vectors of infectious diseases
Alumni: Lenka Zídková, Jan Votýpka, Petr Synek, Ondřej Černý, Jana Szabová
in cooperation with
Ivan Čepička (Department of Zoology) WWW
Petr Voříšek (Czech Society for Ornithology)
Karel Weidinger (Palacky University, Olomouc)
We study vector-parasite-host interactions in bird populations.
The particular aims are:
* Identification of blood parasites, their prevalences and modes of transmission
* Seasonal dynamics of ornithophilic Diptera
* Trypanosome host specificity, life cycle in the vector and experimental transmission
* Biodiversity of avian trypanosomes and haemosporidia
Ongoing project: Avian trypanosomes: Biodiversity, vectors, and factors affecting prevalence
We have microscopically examined ornithophilic bloodsucking Diptera (Culicidae, Simuliidae, Hippoboscidae) for the presence of trypanosomatids in their guts. We dissected 3270 specimens, namely Culex pipiens (n=898), C. modestus (136), Simulium vernum (1455), S. angustipes (221), and Ornithomyia avicularia (524). All insect species have been found infected with trypanosomatids, and the prevalence usually ranged between 4 to 8% but reached 60% in S. vernum. Blackflies and hippoboscids harboured exclusively trypanosomes (both T. cf. avium s.s. ; T.corvi/culicavium group in hippoboscids). Mosquitoes were infected with T. culicavium and T. avium s. l. but also with monoxenous parasites. Only 4% of the isolated parasite strains were monoxenous while the majority were avian trypanosomes, confirming the vectorial status of the studied insects (Svobodová et al., Medical and Veterinary Entomology, accepted).
We examined the role of shared vectors and parasite phylogenetic relationships on the occurrence of haemoparasites in the nesting populations of raptors in two different areas of the Czech Republic. Altogether, 788 nestlings and 258 adult Eurasian sparrowhawks (Accipiter nisus) and 321 nestlings and 86 adult common buzzards (Buteo buteo) were screened. We conclude that phylogenetically unrelated parasites that share the same vectors tend to have similar distributions within the host populations of two different raptor species. (Svobodová et al. 2015).
While studying isolates from birds and vectors, we have found that at least 12 lineages of avian trypanosomes exist, which split into 3 different groups according to the SSU rRNA gene and RAPD analysis. Each group was further subdivided into several lineages, some of them representing already described or putatively distinct species of avian trypanosomes. Only some lineages included isolates from both avian and vector hosts while many consisted only from bird or insect host isolates (Zídková et al. 2012)
We have described a new trypanosome species, T. culicavium, isolated from culicines. Its transmission was achieved using laboratory-bred Culex quinquefasciatus and canaries. Although the occurrence of trypanosomes on the stomodeal valve suggested transmission by bite (Volf et al. 2004), we have demonstrated that it occurs by ingestion. Natural avian host was found among mosquito-eating passerines (Votýpka et al. 2012).
Different height preferences of ornithophilic vectors were demonstrated using CDC traps baited with chickens.Culicine mosquitoes occur at ground while blackflies in canopy level (Černý et al. 2011). This probably influences potential host spectra of transmitted trypanosomes (T. culicavium, T. cf. avium), and is in accordance with respective modes of transmission, namely ingestion by mosquito-eating birds in T. culicavium, and prediuresis into conjunctiva in T. cf. avium from blackflies to raptors.
Ornithophilic insects have been studied in nestboxes. While midges were caught readily, blackflies and mosquitoes were almost absent (Votýpka et al. 2008). Trypanosomes previously reported in nestlings of cavity breeders thus should not belong to T. cf avium, since it is transmitted by blackflies, but rather to T. bennetti, T. culicavium or other species of avian trypanosomes.
By comparing SSU rDNA sequences of raptor and bloodsucking insects isolates, we have confirmed that T. cf. avium is transmitted by blackflies (Votýpka et al. 2002). Infective forms isolated directly from blackflies (Eusimulim spp.) caught in the wild were used to show that transmission occurs by ingestion or prediuresis (Svobodová & Votýpka 2004).
We have isolated several monoxenous kinetoplastids from bloodsucking Diptera. Three new species of monoxenous kinetoplastids were described from biting midges: Herpetomonas ztiplika, H. trimorpha, and Sergeia podlipaevi (Podlipaev et al. 2004, Zídková et al. 2010, Svobodová et al. 2007). Their transmission to laboratory-bred biting midges was possible from sugar.
Svobodová M, Weidinger K, Peške L, Volf P, Votýpka J, Voříšek P. (2014) Trypanosomes and haemosporidia in the buzzard (Buteo buteo) and sparrowhawk (Accipiter nisus): factors affecting the prevalence of parasites. PARASITOLOGY RESEARCH 114: 551-560 PDF
Kristofik J; Darolova A; Griggio M; Majtan J; Okuliarova M ; Zeman M ; Zidkova L; Hoi H. (2013) Does egg colouration signal female and egg quality in reed warbler (Acrocephalus scirpaceus)? (2013) ETHOLOGY ECOLOGY & EVOLUTION 25: 129-143 PDF
Synek P; Albrecht T; Vinkler M; Schnitzer J; Votypka J; Munclinger P (2013) Haemosporidian parasites of a European passerine wintering in South Asia: diversity, mixed infections and effect on host condition. PARASITOLOGY RESEARCH 112: 1667-1677 PDF
Synek P; Munclinger P; Albrecht T ; Votypka J (2013) Avian haemosporidians in haematophagous insects in the Czech Republic. PARASITOLOGY RESEARCH: 839-845 PDF
Zídková l., Szabová J., Čepička I., Svobodová M. (2012) Biodiversity of avian trypanosomes. Infection, Genetics and Evolution 12: 102-112 PDF
Valera F, Zídková L. Reproductive anatomy and fecundity estimation of the haematophagous ectoparasite Carnus hemapterus. Parasitol Research (2012) 110: 1733-1739 PDF
Votýpka J. , Szabová J. , Rádrová J., Zídková L. , Svobodová M. (2011) Trypanosoma culicavium sp. nov., an avian trypanosome transmitted by Culex mosquitoes. International Journal of Systematic and Evolutionary Microbiology 62: 745-754 PDF
Černý O., Votýpka J., Svobodová M. (2011) Spatial feeding preferences of ornithophilic mosquitoes, blackflies and biting midges. Medical and Veterinary Entomology 25: 104-108. PDF
Vinkler M., Schnitzer J., Munclinger P., Votýpka J., Albrecht T. (2010) Haematological health assessment in a passerine with extremely high proportion of basophils in peripheral blood. Journal of Ornithology 151: 841-849
Zídková L., Čepička I., Votýpka J., Svobodová M. (2010) Herpetomonas trimorpha sp. nov. (Trypanosomatidae, Kinetoplastida), a parasite of the biting midge Culicoides truncorum (Ceratopogonidae, Diptera). International Journal of Systematic and Evolutionary Microbiology 60: 2236-2246. PDF
Votýpka J., Synek P. & Svobodová M. (2009) Endophagy of biting midges attacking cavity-nesting birds. Medical and Veterinary Entomology 23: 277–280. PDF
Svobodová M, Zídková L, Čepicka I, Oborník M, Lukeš J, Votýpka J. (2007) Sergeia podlipaevi gen. nov., sp. nov. (Trypanosomatidae, Kinetoplastida): a parasite of the biting midges (Ceratopogonidae, Diptera). International Journal of Systematic and Evolutionary Microbiology 57:423-32. PDF
Volf P., Hajmová M., Sádlová J. ,Votýpka J. (2004) Blocked stomodeal valve of the insect vector: similar mechanism of transmission in two trypanosomatid models. International Journal for Parasitology 34: 1221—1227 PDF
Votýpka J., Svobodová M. (2004) Trypanosoma avium: experimental transmission from black flies to canaries. Parasitology Research 92: 147-151 PDF
Votýpka j., Lukeš J., Oborník M. (2004) Phylogenetic relationship of Trypanosoma corvi with other avian trypanosomes. Acta Protozoologica 43: 225-231 PDF
Modrý D., Votýpka J., Svobodová M. (2004) Note on the taxonomy of Frenkelia microti (Findlay & Midleton, 1934) (Apicomplexa: Sarcocystidae) Systematic Parasitology 58: 185-187 PDF
Svobodová M., Voříšek P., Votýpka J., Weidinger K. (2004) Heteroxenous coccidia (Apicomplexa: Sarcocystidae) in the populations of their final and intermediate hosts: European buzzard and small mammals. Acta Protozoologica 43: 251-260 PDF
Votýpka J., Šimek J., Tryjanowski P. (2003) Blood parasites, reproduction and sexual selection in the red-backed shrike (Lanius collurio). Annales Zoologici Fennici 40: 431-439 PDF
Votýpka J., Oborník M., Volf P., Svobodová M., Lukeš J. (2002) Trypanosoma avium of raptors (Falconiformes): phylogeny and identification of vectors. Parasitology 125: 253-263 PDF
Lukeš J., Votýpka J. (2000) Trypanosoma avium: Novel features of the kinetoplast structure. Experimental Parasitology 96: 178-181 PDF
Mugridge N.B., Morrison
D.A., Johnson A.M., Luton K., Dubey J.P., Votýpka J., Tenter A.M. (1999) Phylogenetic
relationships of the genus Frenkelia: a review of its history and new knowledge
gained from comparison of large subunit ribosomal ribonucleic acid gene sequences.
International Journal for Parasitology 29: 957-972 PDF
Votýpka J., Hypša V., Jirků M., Flegr J., Vávra J., Lukeš J. (1998) Molecular phylogenetic of Frenkelia spp. (Protozoa, Apicomplexa) to Sarcocystis falcatula Stiles 1893: Is the genus Sarcocystis paraphyletic? Journal of Eucaryotic Microbiology 45: 137-141 PDF
Voříšek P., Votýpka J., Zvára K., Svobodová M. (1998) Heteroxenous coccidia increase the predation risk of parasitized rodents. Parasitology 117: 521-524 PDF
Svobodová M., Votýpka
J. (1998) [Occurence of blood protists in raptors (Falconiformes)]. Buteo 10:
Svobodová M. (1997) [Heteroxenous coccidia of raptors and owls from the Czech and Slovak Republics] Buteo 9: 35-42 PDF
Svobodová M. (1996) Sarcocystis from goshawk (Accipiter gentilis) with great tit (Parus major) as intermediate host. Acta Protozoologica 35: 223-226 PDF
Svobodová M., Cibulková M. (1995) Isospora sp. (Apicomplexa: Eimeriidae) of Icterine Warbler (Hippolais icterina, Passeriformes, Sylviidae): The possibility of parents to nestlings transmission. Acta Protozoologica 34: 233-235 PDF
Svobodová M. (1994) Isospora, Caryospora and Eimeria (Apicomplexa: Eimeriidae) of passeriform birds from Czech Republic. Acta Protozoologica 33: 101-108 PDF
Zídková L., Votýpka J., Čepička I., Svobodová M. (2005) How many trypanosome species are there in birds? Alauda 73: 245
Černý O., Votýpka J., Svobodová M. (2005) Passerine trypanosomes: Morphological heterogeneity and spatial distribution of vectors. Alauda 73: 275-6
Votýpka J., Svobodová M., Volf P. (2002) Development of avian trypanosomes in mosquitoes. Journal of Eukaryotic Microbiology, http://www.jeukmic.org/abstr/int/c4/c410.html
Votýpka J., Svobodová M., Volf P., Lukeš J. (2001) Species composition and vectors of avian trypanosomes - molecular evidence. Journal of Eukaryotic Microbiology , www.jeukmic.org/abstr/int/c3/c308.html
Votýpka J., Svobodová M., Voříšek P., Peške L., Lacina D., Volf P. (2000) Blood parasites and haematophagous insects of raptors (Falconiformes) in the Czech Republic. Journal of Eukaryotic Microbiology 47(2): A http://www.jeukmic.org/abstr/int/c2/c208.html
Voříšek P., Diviš T., Svobodová M.: Distribution of Frenkelia parasites in the buzzard andits rodent prey. The Ornithological Notebook of the XXI International Ornithological Congress, Vienna, August 20-25, 1994
Svobodová M., Votýpka
J., Voříšek P. (1998): Frenkelia, buzzards and rodents: multiple mechanisms
of parasite survival strategy. Bulletin of the Scandinavian Society for Parasitology
Votýpka J., Svobodová M., Voříšek P., Peške L., Lacina D., Volf P. (1998): Blood parasites and haematophagous insects of raptors (Falconiformes) in the Czech Republic. Bulletin of the Scandinavian Society for Parasitology 8: 87
Svobodová M., Votýpka J., Voříšek P. (1995): Frenkelia spp. in the populations of their final and intermediate hosts. European J. Protistol. 31: 467. Abstract from the 2nd Eur. Congress of Protistology and 8th Eur. Conference on Ciliate Biology, 21-26 July, Clermont-Ferrand, France