A new paper has been published in cooperation with an Italian laboratory of prof. Alba Silipo!

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Our PhD student Celeste Abreu is the first author of a new paper published in the International Journal of Biological Macromolecules titled:
“Insights into stability, dimerisation, and ligand binding properties of Siglec-7: Isotope labelling in HEK293 cells for protein characterisation by NMR spectroscopy”

3D model of disialylated pentaose bound to Siglec-7_CRD (PDB 2HRL)

The study reports that the extracellular domain of Siglec-7 forms a noncovalent dimer, which may impact ligand binding and signalling. It also describes a method for producing double isotopically labelled Siglec-7 in HEK293 cells, suitable for NMR-based structural studies. This work provides new insights into Siglec-7 biology and a protocol applicable to other glycoproteins.

Abreu, C., Di Carluccio, C., Ječmen, T., Skořepa, O., Bláha, J., Marchetti, R., Silipo, A., Vaněk, O. (2025): Insights into stability, dimerisation, and ligand binding properties of Siglec-7: Isotope labelling in HEK293 cells for protein characterisation by NMR spectroscopy. Int. J. Biol. Macromol. 309, 142672. IF 7.7, DOI: 10.1016/j.ijbiomac.2025.142672