Laboratory Equipment

Confocal microscopes

The Leica TCS SP8 is a high-end inverted confocal microscope designed for the advanced fluorescence imaging of fixed and live biological samples. It offers outstanding sensitivity, resolution, and versatility suitable for various applications, including cell biology, developmental biology, neurobiology, plant sciences, and more.

Manufacturer: Leica Microsystems
Model: Leica TCS SP8 (inverted)
Location: room S39

The Zeiss LSM 880 NLO is an advanced confocal microscope featuring single-photon and multiphoton excitation capabilities. Ideal for high-resolution, deep-tissue imaging, it provides outstanding sensitivity and spectral flexibility, supporting extensive applications in life sciences, particularly neurobiology, developmental biology, and cell biology.

Manufacturer: Carl Zeiss AG
Model: LSM 880 NLO (Inverted, Multiphoton)
Location: room S39

Wide-field microscopes

The Nikon ECLIPSE Ti2 is an advanced inverted wide-field microscope renowned for its exceptional optical performance, expansive 25mm field of view (FOV), and versatility in accommodating a wide range of imaging applications, from routine cell culture observations to complex live-cell imaging studies using advanced JOBs module.

Manufacturer: Nikon Instruments Inc.
Model: Leica TCS SP8 (inverted)
Location: room S39

Stereo microscopes

Lightsheet microscopes

Super-resolution microscopes

Microscope Zeiss Elyra PS.1 uses technologies of structured illumination (SIM) and precise localization (PALM/STORM) to overcome the so-called diffraction barrier, which limits us to the resolution of around 200 nm in conventional light microscopy. Nanoscopy allows us to observe structures with 2-3x higher resolution with SIM and up to 5 nm with localization microscopy. The microscope can use a technology of TIRF (Total Internal Reflection Fluorescence) to increase the signal-to-noise ratio in the space near to cover glass. A set of high-quality lasers and cameras help us to make great super-resolution images.

Other advanced equipment

“Microscopy was performed in the Laboratory of Confocal and Fluorescence Microscopy co-financed by the European Regional Development Fund and the state budget of the Czech Republic, projects no. CZ.1.05/4.1.00/16.0347 and CZ.2.16/3.1.00/21515, and supported by the Czech-BioImaging large RI project LM2018129. Computational resources were supplied by the project “e-Infrastruktura CZ” (e-INFRA LM2018140) provided within the program Projects of Large Research, Development and Innovations Infrastructures”