The Leica TCS SP8 is a high-end inverted confocal microscope designed for advanced fluorescence imaging of fixed and live biological samples. It offers outstanding sensitivity, resolution, and versatility suitable for various applications, including cell biology, developmental biology, neurobiology, plant sciences, and more.
Manufacturer: Leica Microsystems
Model: Leica TCS SP8 (inverted)
Location: room S39
Phone: +420 22195 1943
Technical Specifications
Imaging Technology
- Microscope Type: Inverted laser scanning confocal microscope
- Microscope Stand: Leica DMi-8
- Lasers Available:
- Multiple excitation lasers (405 nm, Argon laser 458, 488, 514 nm, 561 nm, 633 nm)
- Detectors:
- One sensitive Hybrid (HyD) detector for high-quality imaging
- One standard Photomultiplier Tube (PMT) detector
- Objectives Available:
- 10x/0.3 WD 11000 μm (air)
- 20x/0.75 WD 660 μm (multi-immersion options: Oil, Glycerol, Water)
- 63x/1.2 WD 140 μm (high NA water immersion)
- 63x/1.4 WD 300 μm (high NA Oil immersion)
- Autofocus:
- HW – Adaptive Focus Control (AFC)
- SF – Best Focus
Advanced Imaging Capabilities
- Lambda scanning (spectral imaging)
- Multi-channel simultaneous imaging
- Z-stacks and 3D reconstruction
- Time-lapse imaging and real-time observations
- Multiposition, Tile, and Regions scanning
- FRAP (Fluorescence Recovery After Photobleaching)
- FRET (Förster Resonance Energy Transfer)
- MATRIX software module for automated object tracking (e.g., growing roots, oocytes)
Live-Cell Imaging Capabilities
- PECON environmental chamber:
- Temperature control
- CO₂ concentration control for maintaining physiological conditions
- OKO-LAB Bold Line environmental chamber (optionally):
- Temperature control (-5°C – 60°C)
- O2 (0-20%) / CO₂ (1-95%) concentration control for maintaining physiological conditions
User Information
Access Requirements
- Mandatory user registration
- Facility-provided training is required before independent use
- Users must understand and demonstrate the correct setup of Köhler illumination for independent microscope operation
Booking and Usage Policies
- Reservation via online booking system after completing registration and training
- Booking guidelines and facility usage rules provided during training
Price
- Pricing for individual services is determined by the current price list. Should you have specific questions regarding the pricing of your project, please feel free to contact us.