The Zeiss LSM 880 NLO is an advanced confocal microscope featuring single-photon and multiphoton excitation capabilities. Ideal for high-resolution, deep-tissue imaging, it provides outstanding sensitivity and spectral flexibility, supporting extensive applications in life sciences, particularly neurobiology, developmental biology, and cell biology.
Manufacturer: Carl Zeiss AG
Model: LSM 880 NLO (Inverted, Multiphoton)
Location: room S39
Technical Specifications
Imaging Technology
- Microscope Type: Inverted laser scanning confocal microscope with multiphoton and Fluorescence Lifetime (FLIM) capabilities.
- Lasers Available:
- Multiple excitation lasers (405 nm, Argon laser 458, 488, 514 nm, 561 nm, 633 nm)
- Tunable femtosecond Chameleon Ultra II Ti:Sa laser (690–1080 nm) for multiphoton excitation
- Detectors:
- One high-sensitivity 32-channel spectral GaAsP detector
- Two standard PMT detectors
- Two NDD detectors
- B&H FLIM detector
- Objectives Available:
- 5x/0.16 (air)
- 10x/0.45 (air)
- 25x/0.8 (multi-immersion options: Oil, Silicone, Glycerol, Water)
- 40x/1.2 (high NA water immersion)
- 63x/1.2 (high NA water immersion)
- 63x/1.4 (high NA Oil immersion)
Advanced Imaging Capabilities
- Lambda scanning (spectral imaging)
- Multi-channel simultaneous imaging
- Z-stacks and 3D reconstruction
- Time-lapse imaging and real-time observations
- Tile scan, multi positions, and regions
- FRAP (Fluorescence Recovery After Photobleaching)
- Photoactivation experiments
- FRET (Förster Resonance Energy Transfer)
- Two-Photon excitation
- FLIM (Fluorescence Lifetime Imaging Microscopy)
- FLIM-FRET
- FCS & FCCS (Fluorescence Correlation and Cross-correlation Spectroscopy)
- RICS (Rastered Image Correlation Spectroscopy)
- SHG (Second Harmonic Generation)
Live-Cell Imaging Capabilities
- Zeiss environmental chamber (Pecon):
- Temperature control
- CO₂ concentration control for maintaining physiological conditions
- OKO-LAB Bold Line environmental chamber (optionally):
- Temperature control (-5°C – 60°C)
- O2 (0-20%) / CO₂ (0-95%) concentration control for maintaining physiological conditions
User Information
Access Requirements
- Mandatory user registration
- Facility-provided training is required before independent use
- Users must understand and demonstrate the correct setup of Köhler illumination for independent microscope operation
Booking and Usage Policies
- Reservation via online booking system after completing registration and training
- Booking guidelines and facility usage rules provided during training
Price
- Pricing for individual services is determined by the current price list. Should you have specific questions regarding the pricing of your project, please feel free to contact us.