KONFMI – Services

Laboratory Equipment

Confocal microscopes

High-end confocal microscope Leica TCS SP8 with one standard PMT and one sensitive HyD detector. Allows acquisition of fixed and living samples. The environmental chamber PECON with the CO2 regulation provides a suitable atmosphere for living cells experiments. The microscope offers several modes including lambda scanning, FRET and FRAP measurements, and is also equipped with a MATRIX module for object tracking during acquisition (e.g. growing oocytes or roots)

Wide-field microscopes

There is also a great wide-field system Olympus Scan^R available. It is equipped with a stabilized light source, high sensitive Hamamatsu camera, motorized stage, filter, and objective turrets and shutters. It is possible to do high-quality imaging as well as high throughput imaging and mosaic imaging. A high-quality stage chamber with the regulation of temperature (-5 to 65 °C) and gas level (CO2, O2) can maintain also multiwell dishes and allow high throughput imaging under different environmental conditions.

Super-resolution microscopes

Microscope Zeiss Elyra PS.1 uses technologies of structured illumination (SIM) and precise localization (PALM/STORM) to overcome the so-called diffraction barrier, which limits us to the resolution of around 200 nm in conventional light microscopy. Nanoscopy allows us to observe structures with 2-3x higher resolution with SIM and up to 5 nm with localization microscopy. The microscope can use a technology of TIRF (Total Internal Reflection Fluorescence) to increase the signal-to-noise ratio in the space near to cover glass. A set of high-quality lasers and cameras help us to make great super-resolution images.

Ligthsheet microscopes

Zeiss Lightsheet Z.1 microscope is great for rapid acquisition of fixed, living, or cleared samples in selective plane illumination. There are two pairs of 5x and 10x illumination objectives. Detection objective of 5x, 10x, 20x, and 40x and two objectives 20x for clearing  RI 1.38 and 1.45. The chamber size is roughly 2x2x2 cm. The two-channel acquisition is driven by two cooled PCO cameras.