Institute of Geochemistry, Mineralogy and Mineral Resources
Faculty of Science, Charles University
Selected publications related to exobiology
Edwards, H.G.M.; Jehlička, J., Němečková, K. and Culka, A. (2023): Scytonin in gypsum endolithic colonisation: First Raman spectroscopic detection of a new spectral biosignature for terrestrial astrobiological analogues and for exobiological mission database extension. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 292, 122406. DOI: 10.1016/j.saa.2023.122406
Jehlička, J.; Culka, A.; Němečková, K. and Mareš J. (2023): Using Raman spectroscopy to detect scytonemin of epiliths and endoliths from marble, serpentinite and gypsum. Journal of Raman Spectroscopy Early, view. DOI: 10.1002/jrs.6514
Košek, F.; Culka, A.; Rousaki, A.; Vandenabeele, P. and Jehlička, J. (2022): Raman spectroscopy of anhydrous and hydrated aluminum sulfates: Experience from burning coal heaps. Journal of Raman Spectroscopy 53, 1959-1973. DOI: 10.1002/jrs.6420
Jehlička, J. and Culka, A. (2022): Critical evaluation of portable Raman spectrometers: From rock outcrops and planetary analogs to cultural heritage – A review. Analytica Chimica Acta 1209, 339027. DOI: 10.1016/j.aca.2021.339027
Culka, A.; Jehlička, J.; Oren, A.; Rousaki, A. and Vandenabeele, P. (2022): Fast outdoor screening and discrimination of carotenoids of halophilic microorganisms using miniaturized Raman spectrometers. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 276, 121156. DOI: 10.1016/j.saa.2022.121156
Jehlička, J.; Edwards, H.G.M. and Oren, A. (2022): Analysis of brown, violet and blue pigments of microorganisms by Raman spectroscopy. TRAC: Trends in Analytical Chemistry 146, 116501. DOI: 10.1016/j.trac.2021.116501
Košek, F.; Culka, A.; Rousaki, A.; Vandenabeele, P. and Jehlička, J. (2021): Evaluation of miniaturized Raman spectrometers for planetary exploration: From aromatics to amino acids. Icarus 366, 114533. DOI: 10.1016/j.icarus.2021.114533
Němečková, K.; Culka, A.; Němec. I.; Edwards, H.G.M.; Mareš, J. and Jehlička, J. (2021): Raman spectroscopic search for scytonemin and gloeocapsin in endolithic colonizations in large gypsum crystals. Journal of Raman Spectroscopy 52, 2633-2647. DOI: 10.1002/jrs.6186
Němečková, K.; Culka, A. and Jehlička, J. (2021): Detecting pigments from gypsum endoliths using Raman spectroscopy: From field prospection to laboratory studies. Journal of Raman Spectroscopy 53, 630-644. DOI: 10.1002/jrs.6144
Němečková, K.; Jehlička, J. and Culka, A. (2020): Fast screening of carotenoids of gypsum endoliths using portable Raman spectrometer (Messinian gypsum, Sicily). Journal of Raman Spectroscopy 51, 1127-1137. DOI: 10.1002/jrs.5891
Jehlička, J.; Culka, A. and Mareš, J. (2020): Raman spectroscopic screening of cyanobacterial chasmoliths from crystalline gypsum-The Messinian crisis sediments from Southern Sicily. Journal of Raman Spectroscopy 51, 1802-1812. DOI: 10.1002/jrs.5671
Culka, A.; Košek, F.; Oren, A.; Mana, L. and Jehlička, J. (2019): Detection of carotenoids of halophilic prokaryotes in solid inclusions inside laboratory-grown chloride and sulfate crystals using a portable Raman spectrometer: applications for Mars exploration. FEMS Microbiology Letters 366, fnz239. DOI: 10.1093/femsle/fnz239
Jehlička, J.; Culka, A.; Mana, L. and Oren, A. (2019): Comparison of Miniaturized Raman Spectrometers for Discrimination of Carotenoids of Halophilic Microorganisms. Frontiers in Microbiology 10, 1155. DOI: 10.3389/fmicb.2019.01155
Osterrothová, K.; Culka, A.; Němečková, K.; Kaftan, D.; Nedbalová, L.; Procházková, L. and Jehlička, J. (2019): Analyzing carotenoids of snow algae by Raman microspectroscopy and high-performance liquid chromatography. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 212, 262-271. DOI: 10.1016/j.saa.2019.01.013
Jehlička, J.; Culka, A.; Mana, L. and Oren, A. (2018): Using a portable Raman spectrometer to detect carotenoids of halophilic prokaryotes in synthetic inclusions in NaCl, KCl, and sulfates. Analytical and Bioanalytical Chemistry 410, 4437-4443. DOI: 10.1007/s00216-018-1098-3
Košek, F., Culka, A. and Jehlička, J. (2018): Raman spectroscopic study of six synthetic anhydrous sulfates relevant to the mineralogy of fumaroles. Journal of Raman Spectroscopy 49, 1205-1216. DOI: 10.1002/jrs.5363
Barcytė, D; Nedbalová, L.; Culka, A.; Košek, F. and Jehlička, J. (2018): Burning coal spoil heaps as a new habitat for the extremophilic red alga Galdieria sulphuraria. Fottea 18, 19-29. DOI: 10.5507/fot.2017.015
Košek, F.; Culka, A.; Drahota, P. and Jehlička, J. (2017): Applying portable Raman spectrometers for field discrimination of sulfates: Training for successful extraterrestrial detection. Journal of Raman Spectroscopy 48, 1085-1093. DOI: 10.1002/jrs.5174
Košek, F.; Culka, A. and Jehlička, J. (2017): Field identification of minerals at burning coal dumps using miniature Raman spectrometers. Journal of Raman Spectroscopy 48, 1494-1502. DOI: 10.1002/jrs.5161
Culka, A.; Jehlička, J.; Ascaso, C.; Artieda, O.; Casero, C.M. and Wierzchos, J. (2017): Raman microspectrometric study of pigments in melanized fungi from the hyperarid Atacama desert gypsum crust. Journal of Raman Spectroscopy 48, 1487-1493. DOI: 10.1002/jrs.5137
Jehlička, J.; Culka, A. and Košek, F. (2017): Obtaining Raman spectra of minerals and carbonaceous matter using a portable sequentially shifted excitation Raman spectrometer - a few examples. Journal of Raman Spectroscopy 48, 1583-1589. DOI: 10.1002/jrs.5105
Malherbe, C.; Hutchinson, I.B.; McHugh, M.; Ingley, R.; Jehlička, J. and Edwards, H.G.M. (2017): Accurate differentiation of carotenoid pigments using flight representative Raman spectrometers. Astrobiology 17, 351-362. DOI: 10.1089/ast.2016.1547
Jehlička, J.; Culka, A. and Nedbalová, N. (2016): Colonization of snow by microorganisms as revealed using miniature Raman spectrometers—Possibilities for detecting carotenoids of psychrophiles on Mars?. Astrobiology 16, 913-924. DOI: 10.1089/ast.2016.1487
Jehlička, J.; Němec, I.; Varnali, T.; Culka, A.; Svatoš, A.; Frank, O.; Oren, A. and Edwards, H.G.H. (2016): The pink pigment prodigiosin: Vibrational spectroscopy and DFT calculations. Dyes and Pigments 134, 234-243. DOI: 10.1016/j.dyepig.2016.07.018
Culka, A.; Kindlová, H.; Drahota, P. and Jehlička, J. (2016): Raman spectroscopic identification of arsenate minerals in situ at outcrops with handheld (532 nm, 785 nm) instruments. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 154, 193-199. DOI: 10.1016/j.saa.2015.10.025
Jehlička, J.; Edwards, H.G.M.; Němec, I. and Oren, A. (2015): Raman spectroscopic study of the Chromobacterium violaceum pigment violacein using multiwavelength excitation and DFT calculations. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 151, 459-467. DOI: 10.1016/j.saa.2015.06.051
Coccato, A.; Jehlička, J.; Moens, L. and Vandenabeele, P. (2015): Raman spectroscopy for the investigation of carbon-based black pigments. Journal of Raman Spectroscopy 46, 1003-1015. DOI: 10.1002/jrs.4715
Oren, A.; Mana, L. and Jehlička, J. (2015): Probing single cells of purple sulfur bacteria with Raman spectroscopy: carotenoids and elemental sulfur. FEMS Microbiology Letters 362, 1-7. DOI: 10.1093/femsle/fnv021
Jehlička, J.; Edwards, H.G.M.; Osterrothová, K.; Novotná, J.; Nedbalová, L.; Kopecký, J.; Němec, I. and Oren, A. (2014): Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences 372, 20140199. DOI: 10.1098/Rsta.2014.0199
Culka, A.; Osterrothová, K.; Hutchinson, I.; Ingley, R.; McHugh, M.; Oren, A.; Edwards, H.G.M. and Jehlička, J. (2014): Detection of pigments of halophilic endoliths from gypsum: Raman portable instrument and European Space Agency's prototype analysis. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences 372, 20140203. DOI: 10.1098/Rsta.2014.0203
Vandenabeele, P. and Jehlička, J. (2014): Mobile Raman spectroscopy in astrobiology research. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences 372, 20140202. DOI: 10.1098/Rsta.2014.0202
Vítek, P.; Jehlička, J.; Edwards, H.G.M.; Hutchinson, I.; Ascaso, C. and Wierzchos, J. (2014): Miniaturized Raman instrumentation detects carotenoids in Mars-analogue rocks from the Mojave and Atacama deserts. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences 372, 20140196. DOI: 10.1098/Rsta.2014.0196
Jehlička, J. and Edwards, H.G.M. (2014): Raman spectroscopy meets extremophiles on Earth and Mars: studies for successful search of life. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences 372, 20140207. DOI: 10.1098/Rsta.2014.0207
Edwards, H.G.M.; Hutchinson, I.B.; Ingley, R. and Jehlička, J. (2014): Biomarkers and their Raman spectroscopic signatures: a spectral challenge for analytical astrobiology. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences 372, 20140193. DOI: 10.1098/Rsta.2014.0193
Vandenabeele, P.; Edwards, H.G.M. and Jehlička, J. (2014): The role of mobile instrumentation in novel applications of Raman spectroscopy: archaeometry, geosciences, and forensics. Chemical Society Reviews 43, 2628-2649. DOI: 10.1039/C3cs60263j
Culka, A.; Košek, F.; Drahota, P. and Jehlička, J. (2014): Use of miniaturized Raman spectrometer for detection of sulfates of different hydration states - Significance for Mars studies. Icarus 243, 440-453. DOI: 10.1016/j.icarus.2014.08.017
Vítek, P.; Jehlička, J.; Ascaso, C.; Masek, V.; Gomez-Silva, B.; Olivares, H. and Wierzchos, J. (2014): Distribution of scytonemin in endolithic microbial communities from halite crusts in the hyperarid zone of the Atacama Desert, Chile. Fems Microbiology Ecology 90, 351-366. DOI: 10.1111/1574-6941.12387
Jehlička, J.; Edwards, H.G.M. and Oren, A. (2014): Raman spectroscopy of microbial pigments. Applied and Environmental Microbiology 80, 3286-3295. DOI: 10.1128/Aem.00699-14
Hutchinson, I.B.; Parnell, J.; Edwards, H.G.M.; Jehlička, J.; Marshall, C.P.; Harris, L.V. and Ingley, R. (2014): Potential for analysis of carbonaceous matter on Mars using Raman spectroscopy. Planetary and Space Science 103, 184-190. DOI: 10.1016/j.pss.2014.07.006
Marshall, A.O.; Jehlička, J.; Rouzaud, J.N. and Marshall, C.P. (2014): Multiple generations of carbonaceous material deposited in Apex chert by basin-scale pervasive hydrothermal fluid flow. Gondwana Research 25, 284-289. DOI: 10.1016/J.Gr.2013.04.006
Culka, A.; Jehlička, J. and Capoun, T. (2014): Outdoor use of mobile Raman spectrometers to study the solutions and ices of amino acids. Journal of Raman Spectroscopy 45, 179-187. DOI: 10.1002/Jrs.4432
Vítek, P.; Jehlička, J. and Edwards, H.G.M. (2013): Practical considerations for the field application of miniaturized portable Raman instrumentation for the identification of minerals. Applied Spectroscopy 67, 767-778. DOI: 10.1366/12-06774
Vítek, P.; Camara-Gallego, B.; Edwards, H.G.M.; Jehlička, J.; Ascaso, C. and Wierzchos, J. (2013): Phototrophic community in gypsum crust from the Atacama Desert studied by Raman spectroscopy and microscopic imaging. Geomicrobiology Journal 30, 399-410. DOI: 10.1080/01490451.2012.697976
Oren, A.; Bardavid, R.E.; Kandel, N.; Aizenshtat, Z. and Jehlička, J. (2013): Glycine betaine is the main organic osmotic solute in a stratified microbial community in a hypersaline evaporitic gypsum crust. Extremophiles 17, 445-451. DOI: 10.1007/s00792-013-0522-z
Jehlička, J.; Osterrothová, K.; Oren, A. and Edwards, H.G.M. (2013): Raman spectrometric discrimination of flexirubin pigments from two genera of Bacteroidetes. Fems Microbiology Letters 348, 97-102. DOI: 10.1111/1574-6968.12243
Jehlička, J. and Oren, A. (2013): Use of a handheld Raman spectrometer for fast screening of microbial pigments in cultures of halophilic microorganisms and in microbial communities in hypersaline environments in nature. Journal of Raman Spectroscopy 44, 1285-1291. DOI: 10.1002/Jrs.4362
Jehlička, J.; Edwards, H.G.M. and Oren, A. (2013): Bacterioruberin and salinixanthin carotenoids of extremely halophilic Archaea and Bacteria: A Raman spectroscopic study. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 106, 99-103. DOI: 10.1016/j.saa.2012.12.081
Edwards, H.G.M.; Hutchinson, I.B.; Ingley, R.; Parnell, J.; Vítek, P. and Jehlička, J. (2013): Raman spectroscopic analysis of geological and biogeological specimens of relevance to the ExoMars mission. Astrobiology 13, 543-549. DOI: 10.1089/ast.2012.0872
Vítek, P.; Jehlička, J.; Edwards, H.G.M.; Hutchinson, I.; Ascaso, C. and Wierzchos, J. (2012): The miniaturized Raman system and detection of traces of life in halite from the Atacama Desert: Some considerations for the search for life signatures on Mars. Astrobiology 12, 1095-1099. DOI: 10.1089/ast.2012.0879
Vítek, P.; Ali, E.M.A.; Edwards, H.G.M.; Jehlička, J.; Cox, R. and Page, K. (2012): Evaluation of portable Raman spectrometer with 1064 nm excitation for geological and forensic applications. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 86, 320-327. DOI: 10.1016/j.saa.2011.10.043
Vandenabeele, P.; Jehlička, J.; Vítek, P. and Edwards, H.G.M. (2012): On the definition of Raman spectroscopic detection limits for the analysis of biomarkers in solid matrices. Planetary and Space Science 62, 48-54. DOI: 10.1016/j.pss.2011.12.006
Jehlička, J.; Oren, A. and Vítek, P. (2012): Use of Raman spectroscopy for identification of compatible solutes in halophilic bacteria. Extremophiles 16, 507-514. DOI: 10.1007/s00792-012-0450-3
Jehlička, J.; Oren, A. and Edwards, H.G.M. (2012): Raman spectra of osmotic solutes of halophiles. Journal of Raman Spectroscopy 43, 1134-1140. DOI: 10.1002/Jrs.3136
Culka, A.; Jehlička, J. and Strnad, L. (2012): Testing a portable Raman instrument: The detection of biomarkers in gypsum powdered matrix under gypsum crystals. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 86, 347-350. DOI: 10.1016/j.saa.2011.10.047
Vítek, P.; Edwards, H.G.M.; Jehlička, J. and Cox, R. (2011): Evaluation of portable Raman instrumentation for identification of beta-carotene and mellitic acid in two-component mixtures with halite. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 80, 32-35. DOI: 10.1016/j.saa.2011.01.003
Osterrothová, K. and Jehlička, J. (2011): Feasibility of Raman microspectroscopic identification of biomarkers through gypsum crystals. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 80, 96-101. DOI: 10.1016/j.saa.2010.12.085
Osterrothová, K. and Jehlička, J. (2011): Investigation of biomolecules trapped in fluid inclusions inside halite crystals by Raman spectroscopy. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 83, 288-296. DOI: 10.1016/j.saa.2011.08.032
Jehlička, J.; Culka, A.; Vandenabeele, P. and Edwards, H.G.M. (2011): Critical evaluation of a handheld Raman spectrometer with near infrared (785 nm) excitation for field identification of minerals. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 80, 36-40. DOI: 10.1016/j.saa.2011.01.005
Culka, A.; Jehlička, J.; Vandenabeele, P. and Edwards, H.G.M. (2011): The detection of biomarkers in evaporite matrices using a portable Raman instrument under Alpine conditions. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 80, 8-13. DOI: 10.1016/j.saa.2010.12.020
Vítek, P.; Edwards, H.G.M.; Jehlička, J.; Ascaso, C.; De los Rios, A.; Valea, S.; Jorge-Villar, S.E.; Davila, A.F. and Wierzchos, J. (2010): Microbial colonization of halite from the hyper-arid Atacama Desert studied by Raman spectroscopy. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences 368, 3205-3221. DOI: 10.1098/rsta.2010.0059
Osterrothová, K. and Jehlička, J. (2010): Raman spectroscopic identification of phthalic and mellitic acids in mineral matrices. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 77, 1092-1098. DOI: 10.1016/j.saa.2010.08.081
Marshall, C.P.; Edwards, H.G.M. and Jehlička, J. (2010): Understanding the application of Raman spectroscopy to the detection of traces of life. Astrobiology 10, 229-243. DOI: 10.1089/ast.2009.0344
Jehlička, J.; Vítek, P. and Edwards, H.G.M. (2010): Raman spectra of organic acids obtained using a portable instrument at -5 degrees C in a mountain area at 2000 m above sea level. Journal of Raman Spectroscopy 41, 440-444. DOI: 10.1002/Jrs.2450
Jehlička, J.; Vandenabeele, P.; Edwards, H.G.M.; Culka, A. and Čapoun, T. (2010): Raman spectra of pure biomolecules obtained using a handheld instrument under cold high-altitude conditions. Analytical and Bioanalytical Chemistry 397, 2753-2760. DOI: 10.1007/s00216-010-3849-7
Jehlička, J.; Edwards, H.G.M. and Culka, A. (2010): Using portable Raman spectrometers for the identification of organic compounds at low temperatures and high altitudes: exobiological applications. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences 368, 3109-3125. DOI: 10.1098/rsta.2010.0075
Jehlička, J.; Culka, A. and Edwards, H.G.M. (2010): Raman spectra of nitrogen-containing organic compounds obtained in high altitude sites using a portable spectrometer: Possible application for remote robotic Titan studies. Planetary and Space Science 58, 875-881. DOI: 10.1016/j.pss.2010.01.013
Jehlička, J. and Culka, A. (2010): Raman spectra of nitrogen-containing organic compounds obtained using a portable instrument at -15 degrees C at 2860 m above sea level. Journal of Raman Spectroscopy 41, 537-542. DOI: 10.1002/Jrs.2410
Edwards, H.G.M.; Sadooni, F.; Vítek, P. and Jehlička, J. (2010): Raman spectroscopy of the Dukhan sabkha: identification of geological and biogeological molecules in an extreme environment. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences 368, 3099-3107. DOI: 10.1098/rsta.2010.0101
Culka, A.; Jehlička, J. and Edwards, H.G.M. (2010): Acquisition of Raman spectra of amino acids using portable instruments: Outdoor measurements and comparison. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 77, 978-983. DOI: 10.1016/j.saa.2010.08.034
Culka, A. and Jehlička, J. (2010): Raman microspectrometric investigation of urea in calcite and gypsum powder matrices. Journal of Raman Spectroscopy 41, 1743-1747. DOI: 10.1002/Jrs.2643
Vítek, P.; Osterrothová, K. and Jehlička, J. (2009): Beta-carotene-A possible biomarker in the Martian evaporitic environment: Raman micro-spectroscopic study. Planetary and Space Science 57, 454-459. DOI: 10.1016/j.pss.2008.06.001
Vítek, P.; Jehlička, J.; Edwards, H.G.M. and Osterrothová, K. (2009): Identification of beta-carotene in an evaporitic matrix-evaluation of Raman spectroscopic analysis for astrobiological research on Mars. Analytical and Bioanalytical Chemistry 393, 1967-1975. DOI: 10.1007/s00216-009-2677-0
Osterrothová, K. and Jehlička, J. (2009): Raman spectroscopic identification of usnic acid in hydrothermal minerals as a potential Martian analogue. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 73, 576-580. DOI: 10.1016/j.saa.2008.09.005
Jehlička, J.; Vítek, P.; Edwards, H.G.M.; Hargreaves, M.D. and Čapoun, T. (2009): Fast detection of sulphate minerals (gypsum, anglesite, baryte) by a portable Raman spectrometer. Journal of Raman Spectroscopy 40, 1082-1086. DOI: 10.1002/Jrs.2246
Jehlička, J.; Vítek, P.; Edwards, H.G.M.; Hargreaves, M. and Čapoun, T. (2009): Application of portable Raman instruments for fast and non-destructive detection of minerals on outcrops. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 73, 410-419. DOI: 10.1016/j.saa.2008.09.004
Jehlička, J.; Vítek, P.; Edwards, H.G.M.; Hargreaves, M. and Čapoun, T. (2009): Rapid outdoor non-destructive detection of organic minerals using a portable Raman spectrometer. Journal of Raman Spectroscopy 40, 1645-1651. DOI: 10.1002/Jrs.2313
Jehlička, J.; Edwards, H.G.M. and Vítek, P. (2009): Assessment of Raman spectroscopy as a tool for the non-destructive identification of organic minerals and biomolecules for Mars studies. Planetary and Space Science 57, 606-613. DOI: 10.1016/j.pss.2008.05.005
Jehlička, J. and Edwards, H.G.M. (2008): Raman spectroscopy as a tool for the non-destructive identification of organic minerals in the geological record. Organic Geochemistry 39, 371-386. DOI: 10.1016/j.orggeochem.2008.01.005