Laboratory of Regulatory RNA

Open PhD position:

We are offering a PhD position! Please contact us as soon as possible, ideally by April 16th. (The Charles University application deadline for enrollment in the PhD study program is at the end of April). The topic focuses on RNAs associated with RNA polymerase and their role(s) in the regulation of bacterial transcription.

Do your master thesis in our team:

The function of sigma B in mycobacteria

Thesis description:
Sigma factors help bacterial RNA polymerase recognize promoters and initiate transcription. One of the sigma factors in mycobacteria, sigma B, is capable of forming large complexes with RNA polymerase in vitro. However, RNA polymerase in these complexes is inactive and unable to participate in transcription. The aim of this study is to determine whether these RNA polymerase complexes are present in bacteria and how their assembly is regulated. Recently, we discovered an RNA molecule that binds to sigma B and could regulate the assembly of these complexes. As a model organism, we use the non-pathogenic Mycobacterium smegmatis.
Literature:
https://www.nature.com/articles/s41467-023-36113-y
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae081/7606967?login=false

Do your bachelor thesis in our team:

Regulation of transcription in bacteria

For the specific topic of bachelor thesis, please contact us directly.