OBSIL GROUP
Biophysical Chemistry of Protein Complexes

Author: Year: Journal:


Selected publications
Smidova A, Alblova M, Kalabova D, Psenakova K, Rosulek M, Herman P, Obsil T, Obsilova V
14-3-3 protein masks the nuclear localization sequence of caspase-2
FEBS J. 2018 Nov;285(22):4196-4213.
IF = 4.53

Kylarova S, Psenakova K, Herman P, Obsilova V, Obsil T.
CaMKK2 kinase domain interacts with the autoinhibitory region through the N-terminal lobe including the RP insert.
Biochim Biophys Acta. 2018 Jul 24;1862(10):2304-2313.
IF = 3.679

Psenakova K, Petrvalska O, Kylarova S, Santo DL, Kalabova D, Herman P, Obsilova V, Obsil T.
14-3-3 protein directly interacts with the kinase domain of calcium/calmodulin-dependent protein kinase kinase (CaMKK2).
Biochim Biophys Acta-General Subjects. 2018, 1862(7):1612-1625
IF = 3.679

Alblova M, Smidova A, Docekal V, Vesely J, Herman P, Obsilova V, Obsil T
Molecular basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1.
Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):E9811-E9820.
IF = 9.66

Kacirova M, Novacek J, Man P, Obsilova V, Obsil T
Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function.
Biophys J. 2017 Apr 11;112(7):1339-1349
IF = 3.632

Publications by year

2018


Smidova A, Alblova M, Kalabova D, Psenakova K, Rosulek M, Herman P, Obsil T, Obsilova V
14-3-3 protein masks the nuclear localization sequence of caspase-2
FEBS J. 2018 Nov;285(22):4196-4213.
IF = 4.53

Kylarova S, Psenakova K, Herman P, Obsilova V, Obsil T.
CaMKK2 kinase domain interacts with the autoinhibitory region through the N-terminal lobe including the RP insert.
Biochim Biophys Acta. 2018 Jul 24;1862(10):2304-2313.
IF = 3.679

Psenakova K, Petrvalska O, Kylarova S, Santo DL, Kalabova D, Herman P, Obsilova V, Obsil T.
14-3-3 protein directly interacts with the kinase domain of calcium/calmodulin-dependent protein kinase kinase (CaMKK2).
Biochim Biophys Acta-General Subjects. 2018, 1862(7):1612-1625
IF = 3.679

2017


Alblova M, Smidova A, Docekal V, Vesely J, Herman P, Obsilova V, Obsil T
Molecular basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1.
Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):E9811-E9820.
IF = 9.66

Obsil T, Obsilova V
Structural aspects of protein kinase ASK1 regulation.
Adv Biol Regul. 2017 Oct 16. pii: S2212-4926(17)30163-X.
IF = 0

Stevers LM, Sijbesma E, Botta M, MacKintosh C, Obsil T, Landrieu I, Cau Y, Wilson AJ, Karawajczyk A, Eickhoff J, Davis J, Hann MM, O
Modulators of 14-3-3 Protein-Protein Interactions.
J Med Chem. 2017 Oct 19. doi: 10.1021/acs.jmedchem.7b00574.
IF = 6.259

Kalabova D, Smidova A, Petrvalska O, Alblova M, Kosek D, Man P, Obsil T, Obsilova V
Human procaspase-2 phosphorylation at both S139 and S164 is required for 14-3-3 binding.
Biochem Biophys Res Commun. 2017 Nov 18;493(2):940-945.
IF = 2.466

Kacirova M, Novacek J, Man P, Obsilova V, Obsil T
Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function.
Biophys J. 2017 Apr 11;112(7):1339-1349
IF = 3.632

2016


Kylarova S, Kosek D, Petrvalska O, Psenakova K, Man P, Vecer J, Herman P, Obsilova V, Obsil T
Cysteine residues mediate high-affinity binding of thioredoxin to ASK1.
FEBS J. 2016 Oct;283(20):3821-3838
IF = 4.237

Petrvalska O, Kosek D, Kukacka Z, Tosner Z, Man P, Vecer J, Herman P, Obsilova V and Obsil T
Structural Insight into the 14-3-3 Protein-Dependent Inhibition of Protein Kinase ASK1
J Biol Chem 2016 Sep 23;291(39):20753-65
IF = 4.258

2015


Zhao H, Ghirlando R, ..., Kosek D, ..., Obsil T, ..., Schuck P
A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.
PLoS One. 2015 May 21;10(5):e0126420
IF = 3.057

Kacirova M, Kosek D, Kadek A, Man P, Vecer J, Herman P, Obsilova V, Obsil T
Structural Characterization of Phosducin and its Complex with the 14-3-3 Protein
J Biol Chem 2015 Jun 26;290(26):16246-60.
IF = 4.573

2014


Kosek D, Kylarova S, PsenakovaK, Rezabkova L, Herman P, Vecer J, Obsilova V, Obsil T
Biophysical and Structural Characterization of the Thioredoxin-Binding Domain of Protein Kinase ASK1 and its Interaction with Reduced Thioredoxin
J Biol Chem 2014 Aug 29; 289(35):24463-24474
IF = 4.6

Kopecka M, Kosek D, Kukacka Z, Rezabkova L, Man P, Novak P, Obsil T, Obsilova V
Role of the EF-hand like motif in the 14-3-3 protein-mediated activation of yeast neutral trehalase Nth1
J Biol Chem 2014 May 16; 289(20):13948-13961
IF = 4.6

Obsilova V, Kopecka M, Kosek D, Kacirova M, Kylarova S, Rezabkova L, Obsil T.
Mechanisms of the 14-3-3 protein function: regulation of protein function through conformational modulation.
Physiol Res. 2014 Feb 24;63 Suppl 1:S155-64.
IF = 1.531

2013


Vacha P, Zuskova I, Bumba L, Herman P, Vecer J, Obsilova V, Obsil T.
Detailed kinetic analysis of the interaction between the FOXO4–DNA-binding domain and DNA.
Biophysical Chemistry 2013 Sep 15;184:68-78.
IF = 2.283

Macakova E, Kopecka M, Kukacka Z, Veisova D, Novak P, Man P, Obsil T, Obsilova V
Structural basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1
Biochimica Et Biophysica Acta-General Subjects 2013 May;1830(10):4491-4499.
IF = 3.848

2012


Rezabkova L, Kacirova M, Sulc M, Herman P, Vecer J, Stepanek M, Obsilova V, Obsil T
Structural modulation of phosducin by the phosphorylation and the 14-3-3 protein binding
Biophysical Journal 2012 Nov;103(9):1960–1969.
IF = 3.668

Haladova K, Mrazek H, Jecmen T, Halada P, Man P, Novak P, Chmelik J, Obsil T, Sulc M.
The combination of hydrogen/deuterium exchange or chemical cross-linking techniques with mass spectrometry: mapping of human 14-3-3ζ homodimer interface.
J Struct Biol. 2012 Jul;179(1):10-7
IF = 3.361

Veisova D, Macakova E, Rezabkova L, Sulc M, Vacha P, Sychrova H, Obsil T, Obsilova V.
Role of individual phosphorylation sites for the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1.
Biochem J. 2012 May 1;443(3):663-70.
IF = 4.654

2011


Rezabkova L, Man P, Novak P, Herman P, Vecer J, Obsilova V, Obsil T.
Structural basis for the 14-3-3 protein-dependent inhibition of the regulator of G-Protein signaling 3 (RGS3) function.
J Biol Chem. 2011 Dec 16;286(50):43527-36.
IF = 5.328

Obsil T, Obsilova V.
Structural basis of 14-3-3 protein functions.
Semin Cell Dev Biol. 2011 Sep;22(7):663-72.
IF = 6.342

Obsil T, Obsilova V.
Structural basis for DNA recognition by FOXO proteins.
Biochim Biophys Acta. 2011 Nov;1813(11):1946-53.
IF = 4.374

Coddou C, Yan Z, Obsil T, Huidobro-Toro JP, Stojilkovic SS.
Activation and regulation of purinergic P2X receptor channels.
Pharmacol Rev. 2011 Sep;63(3):641-83.
IF = 18.861

Valis K, Prochazka L, Boura E, Chladova J, Obsil T, Rohlena J, Truksa J, Dong LF, Ralph SJ, Neuzil J.
Hippo/Mst1 stimulates transcription of the proapoptotic mediator NOXA in a FoxO1-dependent manner.
Cancer Res. 2011 Feb 1;71(3):946-54.
IF = 7.543

2010


Stojilkovic SS, Yan Z, Obsil T, Zemkova H.
Structural insights into the function of P2X4: an ATP-gated cation channel of neuroendocrine cells.
Cell Mol Neurobiol. 2010 Nov;30(8):1251-8.
IF = 3.226

Veisova, D., Rezabkova, L., Stepanek, M., Novotna, P., Herman, P., Vecer, J., Obsil, T., Obsilova, V.
C-terminal segment of yeast BMH proteins exhibits different structure compared to other 14-3-3 protein isoforms.
Biochemistry 49(18), 3853-3861, 2010
IF = 3.226

Rezabkova, L., Boura, E., Herman, P., Vecer, J., Bourova, L., Sulc, M., Svoboda, P., Obsilova, V., Obsil
14-3-3 protein interacts with and affects the structure of RGS domain of regulator of G-protein signaling 3 (RGS3).
J. Struct. Biol. 170(3), 451-461, 2010
IF = 3.673

Boura E, Rezabkova L, Brynda J, Obsilova V, Obsil T.
Crystal structure of human FOXO4-DBD/DNA complex at 1.9 A resolution reveals new details on FOXO binding to the DNA
Acta Crystallogr D Biol Crystallogr. 2010 Dec;66(Pt 12):1351-7.
IF = 14.103

Friedlova E, Grycova L, Holakovska B, Silhan J, Janouskova H, Sulc M, Obsilova V, Obsil T, Teisinger J.
The interactions of the C-terminal region of the TRPC6 channel with calmodulin.
Neurochem Int. 2010 Jan;56(2):363-6.
IF = 3.541

2009


Silhan J. - Vacha P. - Strnadova P. - Vecer J. - Herman P. - Sulc M. - Teisinger J. - Obsilova V. - Obsil T.
14-3-3 protein masks the DNA binding interface of forkhead transcription factor FOXO4.
Journal of Biological Chemistry, 284(29), 19349-19360, 2009
IF = 5.52

Jindrichova M. - Vavra V. - Obsil T. - Stojilkovic S. S. - Zemkova H.
Functional relevance of aromatic residues in the first transmembrane domain of P2X receptors.
Journal of Neurochemistry, 109(3), 923-934, 2009
IF = 4.5

2008


Grycova L. - Lansky Z. - Friedlova E. - Obsilova V. - Janouskova H. - Obsil T. - Teisinger J.
Ionic interactions are essential for TRPV1 C-terminus binding to calmodulin.
Biochemical and Biophysical Research Communications, 375(4), 680-683, 2008
IF = 2.648

Obsilova V. - Nedbalkova E. - Silhan J. - Boura E. - Herman P. - Vecer J. - Sulc M. - Teisinger J. - Dyda F. - Obsil T.
The 14-3-3 protein affects the conformation of the regulatory domain of human tyrosine hydroxylase.
Biochemistry, 47(6), 1768-1777, 2008
IF = 3.379

Mazna P. - Grycova L. - Balik A. - Zemkova H. - Friedlova E. - Obsilova V. - Obsil T. - Teisinger J.
The role of proline residues in the structure and function of human MT2 melatonin receptor
Journal of Pineal Research, 45(4), 361-372, 2008
IF = 5.056

Obsil T. - Obsilova V.
Structure/function relationships underlying regulation of FOXO transcription factors.
Oncogene, 27(16), 2263-2275, 2008
IF = 7.216

Jelinkova I. - Vavra V. - Jindrichova M. - Obsil T. - Zemkova H.W. - Zemkova H. - Stojilkovic S. S.
Identification of P2X(4) receptor transmembrane residues contributing to channel gating and interaction with ivermectin.
Pflügers Archiv, 465(5), 939-950, 2008
IF = 3.526

Obsilova V, Silhan J, Boura E, Teisinger J, Obsil T.
14-3-3 proteins: a family of versatile molecular regulators.
Physiol Res. 57 Suppl 3:S11-21, 2008. Review.
IF = 1.653

2007


Grycova L. - Lansky Z. - Friedlova E. - Vlachova V. - Kubala M. - Obsilova V. - Obsil T. - Teisinger J.
ATP binding site on the C-terminus of the vanilloid receptor.
Archives of Biochemistry and Biophysics, 465(2), 389-398, 2007
IF = 2.578

Boura E. - Silhan J. - Herman P. - Vecer J. - Teisinger J. - Obsilova V. - Obsil T.
Both the N-terminal loop and wing W2 of the forkhead domain of transcription factor Foxo4 are important for DNA binding.
Journal of Biological Chemistry, 282(11), 8265-8275, 2007
IF = 5.581

2005


Mazna P. - Berka K. - Jelinkova I. - Balik A. - Svoboda P. - Obsilova V. - Obsil T. - Teisinger J.
Ligand binding to the human MT2 melatonin receptor: the role of residues in transmembrane domains 3, 6, and 7.
Biochemical and Biophysical Research Communications, 332(3), 726-734, 2005
IF = 3

Obsilova V. - Vecer J. - Herman P. - Pabianova A. - Sulc M. - Teisinger J. - Boura E. - Obsil T.
14-3-3 Protein interacts with nuclear localization sequence of forkhead transcription factor FoxO4.
Biochemistry, 44(34), 11608-11617, 2005
IF = 3.848

2004


Obsilova V. - Herman P. - Vecer J. - Sulc M. - Teisinger J. - Obsil T.
14-3-3zeta C-terminal stretch changes its conformation upon ligand binding and phosphorylation at Thr232.
Journal of Biological Chemistry, 279(6), 4531-4540, 2004
IF = 6.355

Silhan J. - Obsilova V. - Vecer J. - Herman P. - Sulc M. - Teisinger J. - Obsil T.
14-3-3 protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding.
Journal of Biological Chemistry, 279(47), 49113-49119, 2004
IF = 6.355

Mazna P. - Obsilova V. - Jelinkova I. - Balik A. - Berka K. - Sovova Z. - Ettrich R. - Svoboda P. - Obsil T. - Teisinger J.
Molecular modeling of human MT2 melatonin receptor: the role of Val204, Leu272 and Tyr298 in ligand binding.
Journal of Neurochemistry, 91(4), 836-842, 2004
IF = 4.824

Kubala M. - Obsil T. - Obsilova V. - Lansky Z. - Amler E.
Protein modeling combined with spectroscopic techniques: an attractive quick alternative to obtain structural information.
Physiological Research, 53(Suppl. 1), S187-S197, 2004
IF = 1.14

© 2017 Cenek Albl