Laboratory of bacterial physiology
Group leader: Radovan Fišer
Dept. of Genetics and Microbiology, Charles University
Pinkas D., Fišer R., Kozlík P., Dolejšová T., Hryzáková K., Konopásek I., Mikušová G.
Bacillus subtilis cardiolipin protects its own membrane against surfactin-induced permeabilization
BBA - Biomembranes (2020)
Anna Sukova, Ladislav Bumba, Pavel Srb, Vaclav Veverka, Ondrej Stanek, Jana Holubova, Josef Chmelik, Radovan Fiser, Peter Sebo, Jiri Masin
Negative charge of the AC-to-Hly linking segment modulates calcium-dependent membrane activities of Bordetella adenylate cyclase toxin
BBA - Biomembranes (2020)
Tereza Dolejšová, Albert Sokol, Juraj Bosák, David Šmajs, Ivo Konopásek, Gabriela Mikušová, Radovan Fišer
Colicin U from Shigella boydii forms voltage-dependent pores
Journal of Bacteriology (2019)
Petra Lišková, Ivo Konopásek, Radovan Fišer
Simple way to detect Trp to Tb3+ resonance energy transfer in calcium‑binding peptides using excitation spectrum
Journal of Fluorescence (2018)
Gabriela Seydlová, Albert Sokol, Petra Lišková, Ivo Konopásek, Radovan Fišer
Daptomycin pore formation and stoichiometry depends on membrane potential of target membrane
Antimicrobial Agents and Chemotherapy (2018)
Budil J., Matyska Lišková P., Artemenko A., Ukraintsev E., Gordeev I., Beranová J., Konopásek I., Kromka A.
Anti-adhesive properties of nanocrystalline diamond films against Escherichia coli bacterium: Influence of surface termination and cultivation medium.
Diamond and Related Materials (2018)
Lišková, Petra; Beranová, Jana; Ukraintsev, Egor; et al.
Diamond nanoparticles suppress lateral growth of bacterial colonies
Colloids and Surfaces B (2018)
Vanousova, Katerina; Beranova, Jana; Fiser, Radovan; et al.
Membrane fluidization by alcohols inhibits DesK-DesR signalling in Bacillus subtilis
Biochimica Et Biophysica Acta-Biomembranes (2018)
Seydlová G., Pohl R., Zborníková E., Ehn M., Šimák O., et al.
Lipophosphonoxins II: Design, Synthesis and Properties of Novel Broad Spectrum Antibacterial Agents
Journal of Medicinal Chemistry (2017)
Seydlová G., Beranová J., Bíbová I., Dienstbier A., Držmíšek J., Mašín J., Fišer R., Konopásek I., Večerek B.
The extent of the temperature-induced membrane remodeling in two closely related Bordetella species reflects their adaptation to diverse environmental niches
Journal of Biological Chemistry (2017)
Uttlová P., Pinkas D., Bechyňková O., Fišer R., Svobodová J., Seydlová G.
Bacillus subtilis alters the proportion of major membrane phospholipids in response to surfactin exposure
Biochim Biophys Acta – Biomembranes (2016)
Matyska-Lišková P., Fišer R., Macek P., Chmelík J., Sýkora J., Bednářová L., Konopásek I., Bumba L.
Probing the Ca2+-assisted π-π interaction during Ca2+-dependent protein folding
Soft Matter (2015)
Beranová J, Seydlová G, Kozak H, Benada O, Fišer R, Artemenko A, Konopásek I, Kromka A.
Sensitivity of bacteria to diamond nanoparticles of various size differs in gram-positive and gram-negative cells
FEMS Microbiol Lett. (2014)
Mašín J., Fišer R., Linhartová I., Osička R., Bumba L., Hewlett E.L., Benz R., Šebo P.
Differences in Purinergic Amplification of Osmotic Cell Lysis by the Pore-Forming RTX Toxins Bordetella pertussis CyaA and Actinobacillus pleuropneumoniae ApxIA: The Role of Pore Size
Infection and Immunity (2013)
Seydlová G., Fišer R., Čabala R., Kozlík P., Svobodová J., Pátek M.
Surfactin Production Enhances the Level of Cardiolipin in the Cytoplasmic Membrane of Bacillus subtilis
Biochim Biophys Acta – Biomembranes (2013)
Beranová J., Seydlová G., Kozak H., Potocký Š., Konopásek I., Kromka A.
Antibacterial behavior of diamond nanoparticles against Escherichia coli
Physica Status Solidi B (2012) 1-4
Fišer R., Mašín J., Bumba L., Pospíšilová E., Fayolle C., Basler M., Sadílková L., Adkins I., Kamanová J., Černý J., Konopásek I., Osička R., Leclerc C., Šebo P.
Calcium influx rescues adenylate cyclase-hemolysin from rapid cell membrane removal and enables phagocyte permeabilization by toxin pores
PLoS Pathogens (2012) 8(4): e1002580
Seydlová G., Halada P., Fišer R., Toman O., Ulrych A., Svobodová J.
DnaK and GroEL chaperones are recruited to the Bacillus subtilis membrane after short-term ethanol stress
J. Appl. Microbiol. (2012) 112(4):765-774
Beranová J, Mansilla MC, de Mendoza D, Elhottová D, Konopásek I.
Differences in cold adaptation of Bacillus subtilis under anaerobic and aerobic conditions
J Bacteriol (2010)
Bumba L., Mašín J., Fišer R., Šebo P.
Bordetella adenylate cyclase toxin mobilizes its β2 integrin receptor into lipid rafts to accomplish translocation across target cell membrane in two steps
PLoS Pathogens (2010) 6(5): e1000901
Fišer R., Konopásek I.
Different modes of membrane permeabilization by two RTX toxins: HlyA from Escherichia coli and CyaA from Bordetella pertussis.
Biochim Biophys Acta – Biomembranes (2009)
Beranová J., Jemiola-Rzeminska M., Elhottová D., Strzalka K., Konopásek I.
Metabolic control of the membrane fluidity in Bacillus subtilis during cold adaptation
Biochim Biophys Acta – Biomembranes (2008) 1778(2):445-53
Fišer R., Mašín J., Basler M., Krůšek J., Špuláková V., Konopásek I., Šebo P
Third Activity of Bordetella Adenylate Cyclase (AC) Toxin-Hemolysin: membrane translocation of ac domain polypeptide promotes calcium influx into cd11B+ monocytes independently of the catalytic and hemolytic activities.
J. Biol. Chem. (2007) 282 2808-2820
Mašín J., Basler M., Knapp O., El-Azami-El-Idrissi M., Maier E., Konopásek I., Benz R., Leclerc C., Šebo P.
Acylaton of Lysine 860 Allows Tight Binding and Cytotoxicity of Bordetella Adenylate Cyclase on CD11b-Expressing Cells.
Biochemistry (2005) 44 12759-12766
Martín C, M.-Asuncion-Requero M, Mašín J, Konopásek I, Goni F M , Šebo P, and Ostolaza H
Membrane Restructuring by Bordetella pertussis Adenylate Cyclase Toxin, a Member of the RTX Toxin Family.
J. Bacteriol (2004) 176 3760-3765
Konopásek I., Jaroslav Večeř J, Strzalka, K., Amler E
Short-lived fluorescence component of DPH reports on lipid–water interface of biological membranes.
Chemistry and Physics of Lipids (2004) 130 135–144
Mašín J.,Ivo Konopásek, I., Jaroslava Svobodová, J. and Šebo, P.
Different Structural Requirements for Adenylate Cyclase Toxin Interactions with Erythrocyte and Liposome Membranes.
Biochim Biophys Acta (2004) 1660 144-154
Saint-Pierre-Chazalet M, Plášek J, Konopásek I
A monolayer study of properties of isolated membrane phospholipids of Bacillus subtilis.
Colloids and Surfaces B-Biointerfaces (2002) 23 43-49
Konopásek I, Strzalka K, Svobodová J
Cold shock in Bacillus subtilis: different effect of benzyl alcohol and ethanol on the membrane organization and cell adaptation.
Biochim Biophys Acta (2000) 1464 18-26
Smýkal P, Mašín J, Hrdý I, Konopásek I, Žárský V
Chaperone activity of tobacco HSP18, a small heat-shock protein, is inhibited by ATP.
Plant J (2000) 23 703-713
Konopásek I, Kvasnička P, Heřman P, Linnertz H, Obšil T, Večeř J, Svobodová J, Strzalka K, Mazzanti L, Amler E
The origin of the diphenylhexatriene short lifetime component in membranes and solvents.
Chem Phys Lett (1998) 293 429-435
Opekarová M, Urbanová P, Konopásek I, Kvasnička P, Strzalka K, Sigler K, Amler E
Possible nystatin-protein interaction in yeast plasma membrane vesicles in the presence of ergosterol. A Forster energy transfer study.
Febs Lett (1996) 386 181-184
Konopásek I, Kvasnička P, Amler E, Kotyk A, Curatola G
The transmembrane gradient of the dielectric-constant influences the DPH lifetime distribution.
Febs Lett (1995) 374 338-340
Heřman P, Konopásek I, Plášek J, Svobodová J
Time-resolved polarized fluorescence studies of the temperature adaptation in Bacillus-subtilis using DPH and TMA-DPH fluorescent probes.
Biochim Biophys Acta-Biomembranes (1994) 1190 1-8
Toptygin DD, Svobodová J, Konopásek I, Brand L
Fluorescence decay and depolarization of probes in membranes.
SPIE (1992) 1640 Time resolved spectroscopy in biochemistry III 739 - 751
Toptygin DD, Svobodová J, Konopásek I, Brand L
Fluorescence decay and depolarization in membranes.
J Chem Phys (1992) 96 7919-7930