The inverted confocal Raman microscope WITec Alpha300 Ri is equipped with a
532 nm laser and a highly sensitive Andor detection system, designed for confocal micro-Raman spectroscopy in the visible spectral range. Signal detection is based on a cooled CCD camera optimized for the
UV–VIS region, providing very high detection efficiency already around 400 nm, which enables reliable acquisition of even very weak Raman signals.
The microscope was co-financed as part of the project “Development of Infrastructure for Doctoral Programs at Charles University” with registration number CZ.02.01.01/00/22_012/0005514 and is intended for teaching and student training in modern optical and spectroscopic methods.
Manufacturer: OXFORD Instruments
Model: WITec Alpha300 Ri (inverted)
Location: room S47
Phone: +420 22195 1061
Technical Specifications
Imaging Technology
- Microscope Type: Inverted confocal Raman microscope
- Microscope Stand: Nikon ECLIPSE Ti2 (Manufacturer: Nikon Instruments Inc.)
- Lasers Available:
- 532 nm / 30 mW laser for confocal micro-Raman spectroscopy in the visible spectral range
- Detectors:
- Cooled ImagingSource WOI1-33GX178 CMOS camera optimized for the UV–VIS region (high efficiency from ~400 nm).
- The WITec UHTS 300 VIS is a high-throughput (f/4 aperture) lens-based spectrometer with a 300 mm focal length, designed for ultra-fast, high-sensitivity Raman imaging and spectroscopy paired with an Andor iDus CCD camera
- Objectives Available:
- TU PLAN FLUOR 5x/0.15 WD 2350 μm (air)
- PLAN FLUOR 40x/0.75 WD 660 μm (air)
- TU PLAN FLUOR 50x/0.8 WD 1000 μm (air)
- TU PLAN FLUOR 100x/0.9 WD 1000 μm (air)
Advanced Imaging Capabilities
- Raman Mapping: Rapid determination of chemical composition for each pixel in the image
- Spectral Imaging: Pixel-wise acquisition of fluorescence spectra
- Multi-modal Correlation: Direct correlation of reflectance and absorbance measurements with Raman chemical maps
- High-Resolution Analysis: Label-free chemical analysis with spatial resolution at the diffraction limit of light microscopy
- Confocal Z-sampling: High-quality confocal design allows Raman signal collection from a precisely defined sample volume only
- Sample Protection: Optimized for low laser power to reduce local heating, photobleaching, or damage to biological samples
- Wide-field Navigation: Wide field of view (25 mm FOV) and overview imaging for efficient sample navigation and selection of measurement regions
- Fluorescence: Basic fluorescence for DAPI, FITC, TRITC imaging
Sample preparation
under construction
Live-Cell Imaging Capabilities (optionally, not tested)
- OKO-LAB Bold Line environmental chamber (optionally, not tested):
- Temperature control (-5°C – 60°C)
- O2 (0-20%) / CO₂ (1-95%) concentration control for maintaining physiological conditions
User Information
Access Requirements
- Mandatory user registration
- Facility-provided training is required before independent use
- Users must understand and demonstrate the correct setup of Köhler illumination for independent microscope operation
Booking and Usage Policies
- Reservation via the online booking system after completing registration
- The system can be used only under the operator’s supervision.
- Booking guidelines and facility usage rules were provided during training
Price
- Pricing for individual services is determined by the current price list. Should you have specific questions regarding the pricing of your project, please feel free to contact us.